45results about How to "Promote proliferation" patented technology

This invention belongs to the field of plant extract technology, specifically relating to a plant extract composition for enhancing immunity. By weight, it is composed of the following raw materials: 3-5 parts Astragalus membranaceus extract, 2.5-4 parts Echinacea purpurea extract, and 1.5-3.5 parts Rhodiola rosea extract. This plant extract composition, through the scientific formulation of Astragalus membranaceus, Echinacea purpurea, and Rhodiola rosea, and an enzymatic extraction process, enhances the immunomodulatory effect. The combination of the three extracts increases the secretion of TNF-α and IL-6 by macrophages. Furthermore, the spleen lymphocyte stimulation index (SI) of the three extracts reaches 5.57, and the lymphocyte transformation rate increases from 191% to 457%. These results indicate that this plant extract composition effectively enhances the body's non-specific and cellular immune functions by activating macrophages and promoting T lymphocyte proliferation through multiple pathways, providing more comprehensive immune protection.

The application belongs to the technical field of biological medicine, and discloses an in-situ vaccine type mRNA-tLNP preparation for solid tumor treatment and preparation and application thereof. The preparation is designed by double targets, so that CAR-T kills more tumor cells to overcome tumor heterogeneity, changes the tumor immune microenvironment through autocrine fusion proteins (anti-PD-1 scFv and TGFbetaRII, IL-15 and Flt3L, CD40L), promotes T cell proliferation, and recruits and activates dendritic cells, cross-presents new antigens in the tumor local part, and produces in-situ vaccination effects; circular RNA encoding the above proteins is prepared, and the circular RNA is wrapped by lipid nanoparticles modified by CD3 antibodies, so as to be directly delivered to the body, and a significant solid tumor treatment effect is produced.

The application relates to the technical field of cell culture, in particular to application of Gremlin1 protein in promoting proliferation of adipose-derived cells. The application also provides a culture medium containing the Gremlin1 protein, which is used for culturing the adipose-derived cells, can significantly promote proliferation of the adipose-derived cells, and is significantly superior to a commercial special culture medium.

This invention provides an oyster peptide and its application in enhancing immunity. The oyster peptide has a novel amino acid structure. The oyster peptide can enhance the activity of immune cells, promote the proliferation of immune cells, promote the development and maturation of immune organs, regulate the in vivo immune environment, promote the secretion of immunoglobulins, and improve humoral immunity.

The application discloses a kind of low morbidity feed for California bass antibiotic-free breeding and preparation method thereof, belong to aquatic feed field.The feed is composed of 60~80 parts of basic feed component, 10~20 parts of fermented plant protein, 2~6 parts of functional compound additives.Fermented plant protein is prepared by compound fermentation of butyric acid clostridium and bacillus subtilis from soybean meal, bran, rice bran, brown sugar and the like.Functional compound additives include pre-embedded material and microcapsule coated sodium butyrate, and the pre-embedded material is prepared by embedding treatment of astaxanthin, lutein and rosemary extract with β-cyclodextrin and gum arabic.The application significantly reduces the incidence of intestinal inflammation of California bass through the synergistic effect of fermented plant protein, pre-embedded antioxidant combination and microcapsule sodium butyrate, enhances antioxidant capacity, improves body color, improves the stability of active ingredients and feeding rate, and realizes low morbidity under antibiotic-free breeding conditions.

The present application belongs to the field of foot and ankle surgical implant devices and biomedical material surface engineering technology, and discloses a tibial component of ankle prosthesis with a composite coating, which comprises: a metal substrate which is a bionic trabecular three-dimensional interconnected porous titanium alloy structure, the porosity is 50-80%, the average pore size is 400-900 mu m, and the connected porosity is greater than or equal to 85%, so that the elastic modulus is controlled in the range of 5-20 GPa, and is matched with the cancellous bone of the tibia; a composite functional coating is in situ grown on the surface of the metal substrate, and the composite functional coating comprises a bioactive layer and a functional ion doped layer. The tibial component of the present application comprises a porous titanium alloy metal substrate and a composite functional coating constructed in situ on the surface thereof, the porous titanium alloy substrate is designed in a bionic trabecular structure to form a three-dimensional interconnected porous structure, so as to provide an elastic modulus matched with the cancellous bone of the tibia and promote the growth of bone tissue.

This invention provides a composition comprising milk thistle extract and Akkella myxophilus. This composition can rapidly increase the abundance of Akkella myxophilus in the intestinal environment, promote its proliferation in the intestine, increase the variety and abundance of beneficial intestinal bacteria, colonize beneficial bacteria, and eliminate harmful bacteria. It has the effects of improving intestinal microecology, relieving fatigue, protecting the liver, reducing fat, relieving hangovers, anti-inflammatory, antioxidant, anti-aging, and beautifying the skin.

This invention belongs to the field of saline-alkali land improvement technology, specifically relating to a compound microbial agent and its application in saline-alkali land improvement. This invention prepares a compound microbial agent for improving saline-alkali land using microorganisms such as Chaetomium coccidioides, Bacillus subtilis, Bacillus licheniformis, Bacillus belye, and humic acid. When applied to saline-alkali land improvement, this agent not only significantly improves the soil's physical and chemical properties but also significantly reduces the number of stunted cotton seedlings, increases cotton seed yield, and improves fiber quality.

The application belongs to the field of treating blood vessel-bone coupling imbalance diseases in traditional Chinese medicine, and particularly relates to a composition for treating blood vessel-bone coupling imbalance diseases, a pharmaceutical preparation and application. Geniposide alone or in combination with crocin I can effectively reduce endothelial cell damage under pathological stimulation conditions, reduce the level of cell apoptosis and the degree of oxidative stress, improve the vascular endothelial function state, and can also significantly inhibit osteoclast differentiation, reduce the number of mature osteoclasts, and reduce bone resorption activity, thereby being conducive to restoring the dynamic balance between bone formation and bone resorption.

The application provides an ultrasonic response piezoelectric ionic hydrogel and application thereof, and the hydrogel is formed by cross-linking and solidification of a precursor solution under ultraviolet irradiation; components of the precursor solution include a photosensitive polymer, an ionic polymer, a photoinitiator and a solvent; the photosensitive polymer includes any one or a combination of at least two of polyether F127 diacrylate, polyhydroxyethyl methacrylate, polyethylene glycol dimethyl acrylate, methacrylated gelatin or methacrylated collagen; and the ionic polymer includes any one or a combination of at least two of epsilon-polylysine, polyethyleneimine hydrochloride, polyallylamine hydrochloride, polyquaternary ammonium salt-10 or cationic guar gum. The application uses ultrasonic waves as an external remote driving signal to non-invasively and painlessly activate the piezoelectric ionic effect of the hydrogel, generate detectable periodic voltage and current signals in the hydrogel, and has excellent treatment effect on acne and infected wound repair.

The present application relates to the technical field of medical preparations or cosmetics, in particular to the technical field of preparation of active ingredients of plant origin in medical preparations or cosmetics, and discloses a preparation process of centella asiatica extract, which comprises the following steps: adopting enzyme-assisted hot ethanol extraction method, combining with resin purification technology to extract and obtain a centella asiatica concentrated product from centella asiatica; preparing a centella asiatica extract molecular imprinting polymer resin and filling and loading the resin into a solid-phase extraction empty column to prepare a resin column, and purifying the centella asiatica concentrated product through the resin column to prepare the centella asiatica extract with a purity of up to 99%. The prepared centella asiatica extract has the effects of repair and soothing, can be used as an active ingredient in medical preparations or cosmetics, and is used for preparing a gel preparation for treating skin damage, or is added into a repair skin care product to promote skin barrier repair and soothe damaged parts, so that the medical-grade skin care effect and daily care are combined.

The present invention discloses a medicated and edible homologous formula food for improving qi deficiency constitution and its preparation process, belonging to the technical field of functional foods. This formula food uses multiple medicated and edible homologous raw materials such as Ganoderma lucidum, Codonopsis pilosula, Astragalus membranaceus, Lentinula edodes, Poria cocos, Dolichos lablab, etc., and adds prebiotic components such as fructo-oligosaccharide, xylo-oligosaccharide, inulin, etc. It strictly follows the compatibility principle of monarch, minister, assistant and guide, and scientifically formulates the prescription in combination with the law of generation, restriction, control and transformation of zang-fu organs, qi and blood. The present invention solves the problems that existing foods for improving qi deficiency constitution have weak qi-tonifying power, incomplete conditioning, and do not consider the secondary blood deficiency, blood stasis and qi stagnation caused by qi deficiency. It realizes qi-tonifying while taking into account soothing the liver and regulating qi, nourishing blood and harmonizing blood, promoting digestion and relieving stagnation, and regulates the intestinal flora through prebiotics to promote the generation of primordial qi in the lung and the generation and transformation of qi and blood. The preparation process adopts water decoction extraction, concentration and drying, and then compounding with prebiotics, and can be made into beverages or solid beverages. The process is simple and suitable for industrial production. The raw materials are all both medicinal and edible products, and long-term consumption has a significant effect on improving qi deficiency constitution.

This invention belongs to the field of probiotic screening and application technology, specifically relating to a strain of *Lactobacillus paracasei* and its application in the prevention and treatment of colonic polyps and colon cancer. The *Lactobacillus paracasei*, named *Lactobacillus paracasei* V85, was deposited on January 15, 2026, at the China Center for Type Culture Collection (CCTCC), Wuhan University, China, with accession number CCTCC NO: M2026124. The *Lactobacillus paracasei* V85 provided by this invention is sensitive to common antibiotics and has good biosafety; it possesses strong antioxidant function and a strong inhibitory effect on hemolytic streptococci; it can produce a strong agglutination effect with hemolytic streptococci, which is beneficial for inhibiting the colonization of hemolytic streptococci in the intestine, reducing the risk of colonic polyps and colon cancer caused by hemolytic streptococci, and can promote the proliferation of *Akkermansia myxophilus*. It can be used to prevent and treat colonic polyps and colon cancer caused by hemolytic streptococci, and has broad application prospects.

The application discloses application of a PI3K-gamma-delta signal path inhibitor in preparation of a product for treating central nervous system leukemia and the product, and solves the technical problem that the prior art lacks a molecular targeting product for acute lymphoblastic leukemia with CNSL which has better effect. The application comprises: application of the PI3K-gamma-delta signal path inhibitor in preparation of a product for treating acute lymphoblastic leukemia with central nervous system leukemia, and application of the PI3K-gamma-delta signal path inhibitor in preparation of a product for inhibiting proliferation, invasion and migration of acute lymphoblastic leukemia with central nervous system leukemia. The application also comprises a product of the PI3K-gamma-delta signal path inhibitor. The application discloses that central nervous system leukemia is closely related to an expression level of MSLN, and the PI3K-gamma-delta signal path inhibitor can inhibit the infiltration degree of acute lymphoblastic leukemia with central nervous system leukemia by reducing the expression level of MSLN, and prolong the survival time.

The present application relates to the technical field of bioactive glass, in particular to a preparation method and application of strontium-magnesium-doped bioactive glass, on the basis of selecting suitable raw materials, the co-precipitation method is adopted, strontium element is doped, and magnesium oxide is compoundedly added, so that the prepared bioactive glass has regular properties, good dispersible spherical particles, faster and stronger hydroxyapatite formation capacity, faster ion release rate, and can release calcium, phosphorus and silicon ions in simulated body fluid, form more hydroxyapatite, promote the proliferation and stability of bone cells, and stimulate new bone formation. The strontium-magnesium-doped bioactive glass is used in cooperation with mineral trioxide aggregate (MTA), so that cell adhesion, expression of bioactive enzymes, activity of fibroblasts can be promoted, and the strontium-magnesium-doped bioactive glass can be applied to the repair of human bone and dental pulp tissue.

The invention discloses a preparation method and application of a probiotic-entrapped collagen composite nano-carrier. The preparation method is characterized by comprising the following steps: mixing a fish gelatin protein solution and a polysaccharide solution, adjusting the pH value to be alkaline, heating to carry out glycosylation reaction, and purifying to obtain glycosylated fish gelatin protein. Mixing and emulsifying the glycosylated fish gelatin protein solution serving as a water phase and the oil phase by adopting a reversed-phase emulsification method to form a W / O type emulsion; adding a probiotic bacterial suspension, uniformly stirring, adding a cross-linking agent, carrying out a cross-linking reaction, carrying out solid-liquid separation, washing, and drying, so as to obtain the probiotic-entrapped collagen composite nano-carrier; the preparation method has the advantages that precise and slow-release release in intestinal tracts is realized, and the survival rate and bioavailability of probiotics are remarkably improved.

The application discloses an antibody of lncRNA UPK1A-AS1 and a preparation method and application thereof, and relates to the technical field of genetic engineering. The immunogen comprises a polypeptide with an amino acid sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 2. The antibody prepared by immunizing animals with the immunogen can specifically recognize a polypeptide coded by lncRNA UPK1A-AS1, and can also be used for preventing and treating liver cancer.

The application discloses ALDH1A1 The application discloses a gene and a protein in application of promoting hair follicle cell proliferation, and belongs to the technical field of functional gene application. ALDH1A1 The application discloses that overexpression of the gene can significantly promote hair follicle cell proliferation, and the ALDH1A1 protein is expected to be used for preparing a product for promoting hair follicle cell proliferation or damage repair, thereby providing a new and safe candidate preparation for improving the problem of hair loss. ALDH1A1 The application discloses that the expression amount of the gene in skin tissue is significantly higher than that in other tissues; compared with hair follicles in a regression period and a resting period, the expression amount of the gene in hair follicles in a growth period is the highest; and the structural and composition analysis results of the ALDH1A1 protein are provided.

The present application relates to the technical field of genetic engineering, and particularly relates to application of miR-27b-3p in preparation of products for regulating cell proliferation and testosterone synthesis. The present application constructs Inhibitor and Mimics sequences with miR-27b-3p gene as a target, and for the first time proposes that overexpression of miR-27b-3p gene can significantly inhibit proliferation and testosterone synthesis of goat testicular interstitial cells, and inhibition of miR-27b-3p gene can significantly promote proliferation and testosterone synthesis of goat testicular interstitial cells. The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating proliferation and testosterone synthesis of goat testicular interstitial cells with miR-27b-3p gene as a target.

This invention discloses an extracellular polysaccharide DSP-1 from the shell of a deep-sea fungus, *Subseptospora*, and its applications. Didymella keratinophila The fermentation product of SCAU273 was isolated and purified to obtain an extracellular polysaccharide DSP-1. This polysaccharide has a molecular weight of 4116 Da, a bibranched structure, and is mainly composed of glucose polymers. Further studies showed that DSP-1 can enhance the activity and promote the proliferation of RAW 264.7 macrophages over a wide concentration range (25–400 μg / mL). Furthermore, at certain concentrations, it dose-dependently promoted the secretion of NO, TNF-α, IL-6, and IL-1β by RAW 264.7 macrophages, exhibiting good immunomodulatory activity against RAW 264.7 macrophages. It shows broad prospects for the production and widespread application of environmentally friendly marine biological agents.

This invention discloses a method and its application for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites by adding carrageenan to a semi-solid culture medium. The method uses *Propionibacterium f. reudenreichii* subsp. *shermanii* NBRC No. 12426 as the experimental strain and *Pichia anomala* SICC 2.894 as the indicator strain. It discloses a method for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites against the indicator strain *Pichia anomala* by adding carrageenan to the culture medium. Based on the culture conditions and method of *Propionibacterium f. reudenreichii* subsp. *shermanii* in this invention, the optimal amount of carrageenan added is selected as 0.6%, which significantly enhances the antibacterial activity of the *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites.

This invention discloses a core-shell microneedle loaded with silver nanoparticles and growth factors, its preparation method, and its application, belonging to the field of biomedical technology. The core-shell microneedle includes a backing layer and an array of needle tips disposed on the backing layer; the needle tips in the needle tip array have a core-shell structure, with the shell layer of the needle tip loaded with protein-Ag2O2 composite nanoparticles and the core layer of the needle tip loaded with growth factors. The preparation method of the protein-Ag2O2 composite nanoparticles is as follows: a protein aqueous solution is mixed with a silver nitrate solution, and under alkaline conditions, it is reacted with a hydrogen peroxide solution to obtain the protein-Ag2O2 composite nanoparticles. The core-shell microneedles prepared by this invention, through hierarchical loading of silver peroxide nanoparticles and growth factors, respond to the microenvironment of infected skin wounds and perform programmed release, effectively preventing bacterial infection, promoting cell proliferation and angiogenesis, and driving tissue repair, effectively solving the problem of stagnant healing of infected wounds.

This invention discloses a neutral homogeneous polysaccharide from Rehmannia glutinosa, its preparation method, and its applications, belonging to the field of traditional Chinese medicine preparation technology. The polysaccharide is mainly composed of galactose, glucose, and fructose in a molar ratio of 2.72:1.17:1.11, with a weight-average molecular weight of 2152 Da. The neutral polysaccharide from Rehmannia glutinosa can exert a therapeutic effect on osteoporosis by increasing bone mineralization and bone density; it can also promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), showing significant application effects in the preparation of stem cell therapy drugs for bone defects. The neutral homogeneous polysaccharide from Rehmannia glutinosa shows great application potential in the preparation of drugs for treating osteoporosis and as a stem cell therapy (especially bone regeneration therapy based on bone marrow mesenchymal stem cells). This provides new candidate substances and research directions for developing efficient and safe treatment strategies for bone metabolic diseases derived from traditional Chinese medicine.

This invention belongs to the field of functional probiotic screening and application technology, specifically involving a novel strain of *Lactobacillus plantarum* (…). Lactiplantibacillus plantarum The *Lactobacillus plantarum* strain was deposited on January 15, 2026, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M202026133. This strain exhibits strong antioxidant capacity, significant inhibitory and flocculating effects against hemolytic streptococci, and can significantly reduce the levels of pro-inflammatory cytokines IL-6 and IL-1β induced by hemolytic streptococci while increasing the level of the anti-inflammatory cytokine IL-22. It has no toxic effects on the body, good safety profile, and can be used to prevent colonic polyps and colon cancer caused by hemolytic streptococci, showing broad application prospects.

This invention provides a non-contact, adjustable alternating magnetic field-eddy current coupled laser shock strengthening device and method for improving hydrogen embrittlement resistance. The device includes a laser shock strengthening unit, a magnetic field generating unit, a driving unit, and a position adjustment unit. The magnetic field generating unit has alternating N and S pole permanent magnets, which are driven to rotate by the driving unit. This induces an alternating magnetic field and closed eddy currents within the metal workpiece. The magnetic field strength, eddy current density, and temperature rise are synergistically controlled by adjusting the air gap and rotation speed. This invention couples a multi-directional alternating electromagnetic field during laser shock, promoting the activation of multiple slip systems and dislocation dispinning, improving the surface microstructure uniformity and residual compressive stress distribution, effectively inhibiting hydrogen intrusion and enrichment, delaying the initiation and propagation of hydrogen-induced cracks, and improving the workpiece's resistance to hydrogen embrittlement. The device has no contact electrodes and is suitable for complex curved surfaces and large-area workpieces.

This invention discloses a collagen-based anti-corrosion coating with tissue repair properties, its preparation method, and its applications, belonging to the field of medical coating technology. This invention designs a novel self-healing anti-corrosion coating and loads collagen onto the coating using excipients, achieving a deep integration of anti-corrosion protection and biological function. The prepared collagen-based anti-corrosion coating is firmly bonded to a magnesium alloy substrate via covalent bonds. After 4 weeks of in vivo implantation, no cracking or detachment was observed, the stent structure maintained good integrity, and it provides continuous radial support, offering sufficient time for vascular repair. This provides a feasible solution for the clinical translation of next-generation biodegradable magnesium-based vascular stents.

The application provides a glycosyl-modified fusion protein, a nucleic acid molecule, an expression vector, a host cell and application. The first aspect of the application provides a glycosyl-modified fusion protein, comprising a murine Fc variant and a hepatitis B virus antigen, wherein the murine Fc variant is obtained by amino acid mutation and non-mammalian glycosylation modification on a murine Fc fragment; the murine Fc variant comprises at least one of alanine at position 223, alanine at position 228, alanine at position 230, leucine at position 330 and glutamic acid at position 332; the glycosylation modification does not comprise sialic acid modification; and the position numbering is according to the EU numbering system. The fusion protein can bind to DC cells, activate DC cells and promote the proliferation and activation of specific T cells.

The application discloses a cucumber seed oil extract, a preparation method and application thereof, cosmetics and medicines. The method comprises the following steps: providing dried cucumber seeds; seed oil extraction, comprising squeezing the dried cucumber seeds or performing supercritical carbon dioxide extraction after crushing to obtain crude cucumber seed oil; seed oil purification, comprising removing water-soluble organic acids in the crude cucumber seed oil to obtain an oil phase liquid; deodorization, comprising deodorizing the purified oil phase liquid to obtain deodorized crude cucumber seed oil; degreasing, comprising removing solid esters in the deodorized crude cucumber seed oil to obtain degreased crude cucumber seed oil; and impurity removal and drying of the degreased crude cucumber seed oil to obtain the cucumber seed oil extract. The preparation method of the cucumber seed oil extract is capable of obtaining the cucumber seed oil extract through drying, seed oil extraction, seed oil purification, deodorization, degreasing, impurity removal and drying in sequence, and has the advantages of simple operation, high yield, low cost, stable product quality, green and environment-friendly process and suitability for large-scale production.

The invention relates to the technical field of biological medicine and gene therapy, in particular to a nucleic acid delivery system for targeting pancreatic beta cells and application of the nucleic acid delivery system. The composition comprises a nano-composite formed by a cationic polymer modified by a targeting ligand and siRNA (small interfering Ribonucleic Acid), the targeting ligand is a GLP-1 receptor targeting peptide; the cationic polymer is a PEI-PFA polymer; and the siRNA is the siRNA of the target DYRK1A (DYRK1A). The invention provides a brand-new pancreatic beta cell targeted delivery platform for gene therapy of type 2 diabetes mellitus. The platform realizes beta cell function regeneration by accurately regulating and controlling DYRK1A gene expression, has targeting property, safety and high efficiency, and provides a new strategy with wide clinical application prospect for diabetes treatment.