1298results about How to "Promote proliferation" patented technology

The invention relates to a high-activity recombinant XVII type collagen as well as a rapid preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 1, and the nucleotide sequence of the high-activity recombinant XVII type collagen is as shown in SEQ ID NO. 2. The invention also provides a preparation method and application of the recombinant XVII type collagen. The high-activity recombinant XVII type collagen has good biological activity, has good effects of promoting cell adhesion, cell proliferation and cell migration, and can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines. The high-activity recombinant XVII type collagen gene is integrated to different positions of a yeast genome through three different vectors, and the copy number of a target gene is increased, so that the expression quantity of the recombinant XVII type collagen is remarkably increased, and the yield of the high-activity recombinant XVII type collagen is effectively increased.

The invention belongs to the technical field of optimized coding genes, and discloses a recombinant human I-type collagen as well as a preparation method and application thereof, and the amino acid sequence of the recombinant human I-type collagen is shown as SEQ ID No.1. The recombinant I-type collagen provided by the invention is relatively high in expression quantity, has relatively good cell migration promoting, cell proliferation promoting and cell adhesion promoting properties, is high in safety, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention relates to the field of microorganisms, in particular to bifidobacterium adolescentis YG3912, inactivated thalli, an extract, a culture, a culture supernatant, a fermentation product and / or a fermentation supernatant of the bifidobacterium adolescentis YG3912, an application of the bifidobacterium adolescentis YG3912 in preparation of a composition for promoting skin repair, reducing skin inflammation or allergy, improving skin quality and improving skin quality. The invention also relates to application of the composition in products for resisting skin aging and enhancing skin immune function. The bifidobacterium adolescentis YG3912 and the related products thereof have the advantages that the bifidobacterium adolescentis YG3912 and the related products of the bifidobacterium adolescentis YG3912 are high in skin cell repairing capacity, skin inflammation relieving, inhibiting or eliminating capacity and remarkable in skin aging resisting capacity, so that the bifidobacterium adolescentis YG3912 and the related products of the bifidobacterium adolescentis YG3912 are expected to be used for skin health care such as skin repairing, inflammation relieving and light aging resisting, and the bifidobacterium adolescentis YG3912 and the related products thereof have remarkable application value and market potential.

The invention discloses a recombinant humanized XVII type collagen as well as an expression vector and a genetically engineered bacterium thereof. Four functional fragments with high activity are obtained through screening, one of the fragments is optimized and then combined according to a certain sequence to obtain recombinant humanized XVII type collagen JYC1703, or one of the fragments is optimized and then combined according to a certain sequence, and GPP sequences are fused at an amino terminal and a carboxyl terminal respectively to obtain recombinant humanized XVII type collagen JYC1701 and JYC1702, and the recombinant humanized XVII type collagen JYC1701 and JYC1702 are combined according to a certain sequence to obtain the recombinant humanized XVII type collagen JYC1701 and JYC1702. After codon optimization, amplifying and cloning into an expression vector, transforming into pichia pastoris competent cells after enzyme digestion linearization, and screening high-copy recombinants through G418 resistance gradient, so as to obtain pichia pastoris genetically engineered bacteria for expressing recombinant humanized XVII type collagen. The recombinant humanized XVII type collagen JYC1701, JYC1702 and JYC1703 disclosed by the invention show excellent functions of promoting cell adhesion, cell proliferation and cell migration, and are suitable for preparing products such as skin care products or medical instruments and the like.

The invention provides a tripeptide for promoting bone growth, the tripeptide is VPF, the amino acid sequence of the tripeptide is Val-Pro-Phe, the molecular weight of the tripeptide is 361.44 Da, and the tripeptide belongs to the technical field of functional bioactive peptides. The tripeptide can be prepared from egg yolk protein through enzymolysis separation and purification, can also be obtained through chemical solid-phase synthesis, has a good effect of promoting bone growth, can remarkably improve the growth and proliferation level of osteoblasts in a body, promotes synthesis and secretion of bone matrixes, and has a good application prospect. Meanwhile, the secretion of insulin-like growth factors (IGF-1), transforming growth factors (TGF-beta1) and I-type procollagen amino-terminal peptide (PINP) in the body is efficiently promoted, and good market prospects are achieved.

The invention belongs to the technical field of preparation of microorganisms and cosmetics, and particularly relates to a strain composition, radix paeoniae rubra fermentation liquor prepared from the strain composition and application of the strain composition in cosmetics. Specifically, Pseudoalteromonas sp. SE9 and Schizoscharomyces pombe JM2-16 are utilized to perform fermentation treatment on radix paeoniae rubra root powder, and the finally obtained radix paeoniae rubra fermentation liquor has multiple effects of resisting oxidation, promoting proliferation and migration of keratinocytes, promoting skin injury repair, resisting allergy, resisting inflammation, relieving and the like, is high in safety, can be used as a cosmetic component, and can be used as a cosmetic preparation. Therefore, the method has good practical application value.

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

The invention belongs to the technical field of metal organic frameworks, and particularly relates to a high-performance photocatalytic metal organic framework antibacterial material as well as a preparation method and application thereof. The preparation method comprises the following steps of: growing a metal organic framework UiO 66 which takes a cerium element as a metal node on a single-layer graphene oxide UiO66 (at) GO in situ, loading arginine small molecules in the MOF framework to obtain a high-performance photocatalytic MOF antibacterial material UiO66 (at) GO (at) arg, forming a nano hydrogel with a stable three-dimensional structure with chitosan, and performing antibacterial treatment by using the nano hydrogel as a wound dressing. The Ce-based MOF composite QCS / GO-UiO 66-coated arg / PNIPAm hydrogel prepared by the preparation method disclosed by the invention has excellent properties such as elasticity, adhesive power, water absorption and biocompatibility; and under illumination, the photocatalyst has an excellent photocatalytic sterilization effect, and has a good killing effect on escherichia coli and staphylococcus aureus.

The invention provides a large-scale culture method of NK cells and an adopted culture medium, and belongs to the technical field of NK cell culture. And adding fetal calf serum, penicillin, streptomycin and the stimulation amplification promotion magnetic microspheres into the ACTM culture medium, and uniformly stirring and mixing to obtain the culture medium. The large-scale culture method of the NK cells can efficiently activate and amplify the NK cells, is free of exogenous serum and autoserum, safe and effective, can well promote proliferation of the NK cells and prolong the activity of the NK cells, is safe and free of side effects, and has a wide application prospect.

The invention provides a preparation method of a mixed pilatory of active peptide and stem cell exosome, and belongs to the technical field of pilatory preparation. The preparation method comprises the following steps: extracting the stem cell exosome; extracting an active extract; preparing an exosome entrapped active extract; preparing the liposome loaded active peptide; preparing the liposome loaded active peptide modified by the gallic acid modified chitosan; and preparing the mixed hair restorer. The prepared mixed hair restorer can provide a suitable growth environment for hair follicles and promote hair growth, the synergistic effect of the active peptide and the exosome can provide abundant signal molecules and enhance the stimulation effect on hair follicle cells, and the active peptide and the exosome can promote angiogenesis and increase the blood flow volume of scalp, so that the hair follicle can be effectively promoted. More nutrition and oxygen are provided for hair follicles, and the growth of hair is effectively promoted.

The invention discloses a preparation method of a metal-based nano-enzyme and application of the metal-based nano-enzyme in treatment of periodontitis, the nano-enzyme combines two metal bases, and the nano-enzyme exerts anti-inflammatory, vascularization and osteogenesis effects through advantage complementation to treat periodontitis. The nano-enzyme for treating periodontitis has excellent anti-oxidation / anti-inflammatory performance, exerts an ROS elimination function in a specific environment, responds to a periodontitis pathology microenvironment to release metal ions, exerts a growth factor-like function of the metal ions and realizes repair of blood vessels and bone tissues. The multifunctional nano material has the advantages of simple process, low cost, high activity and the like, and has a good treatment effect on periodontitis.

The invention relates to the technical field of recombinant protein expression, in particular to recombinant human-derived III-type collagen as well as a preparation method and application thereof. The invention provides a recombinant human source III type collagen. The amino acid sequence of the recombinant human source III type collagen is shown as SEQ ID NO. 1. The recombinant human-derived III-type collagen prepared by the invention can be better applied to a human body, better exerts biological functions of the collagen, avoids repulsive interaction, is high in safety, can effectively promote cell proliferation and oxidation resistance, and is high in expression yield, stable in batches and good in quality; the recombinant human III type collagen prepared by the invention has the potential of being applied to preparation and production of biological medicines and cosmetics.

The invention discloses a milk-derived polypeptide sequence for promoting skeletal muscle cell proliferation and application of the milk-derived polypeptide sequence, and belongs to the field of bioactive peptides. The milk-derived bioactive polypeptide RHPHPHLSF provided by the invention has the effect of promoting muscle cell proliferation, and is expected to be used for preparing medicines for promoting muscle cell proliferation and preventing sarcopenia. The milk-derived bioactive polypeptide RHPHPHLSF is convenient to synthesize, can be industrially produced, and has a good application prospect in the fields of foods, medicines, cosmetics and the like.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention provides a tetrapeptide for promoting bone growth, the amino acid sequence of the tetrapeptide is Glu-Pro-Gly-Phe (EPGF), the molecular weight of the tetrapeptide is 448.48 Da, the tetrapeptide can be prepared from egg yolk protein through protease enzymolysis, separation and purification and can also be obtained through chemical solid-phase synthesis, and the tetrapeptide belongs to the technical field of functional bioactive peptides. The tetrapeptide prepared by the invention can promote the growth of osteoblasts in a body and improve the secretion level of insulin-like growth factor-1 (IGF-1), transforming growth factor-beta1 (TGF-beta1) and I-type procollagen amino terminal peptide (PINP) in cells, so that the effect of promoting bone growth is achieved, and a new functional component is provided for a product for improving bone growth.

The invention relates to the technical field of skull repair, and discloses a personalized 3D printing porous composite hydroxyapatite coating skull repair body which comprises a truss structure, a plurality of porous areas are distributed on the truss structure, bionic bone trabecula structures are integrally formed in the porous areas, the truss structure is composed of hollow pipes communicating with one another, and the hollow pipes communicate with one another. A plurality of small holes and micro holes which are communicated with the interior of the hollow pipe are formed in the hollow pipe, and a composite coating of a hydroxyapatite coating and titanium is sprayed on the porous area; the invention provides a personalized 3D printed skull restoration with a porous composite hydroxyapatite coating. The problem that the biocompatibility, the bone induction capability and the bone ingrowth effect of an existing skull restoration need to be improved is solved.

The invention relates to the field of cell biology, and particularly discloses an exosome as well as a preparation method and application thereof. The method for preparing the exosome comprises the following steps: (1) introducing nucleotide sequences for coding a signal peptide TPA and an epidermal growth factor into an AAVS1 region of a host cell, such as a sequence shown in SEQ ID No.9 and a sequence shown in SEQ ID No.10, so as to obtain a modified host cell; and (2) collecting the exosome secreted by the modified host cell. The expression level of EGF mRNA in the exosome can be remarkably improved, so that the obtained exosome has a better effect in skin wound treatment.

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The invention discloses a preparation method of a zinc-dihydromyricetin nano-enzyme and application of the zinc-dihydromyricetin nano-enzyme in preparation of drugs for promoting healing of diabetic wounds, and the zinc-dihydromyricetin metal polyphenol nano-enzyme (Zn-DHM) is obtained by coordinating a metal element zinc (Zn) and a natural product dihydromyricetin (DHM) through a one-step method by taking PVP as a surfactant. The Zn-DHM nano-enzyme prepared by the invention has antioxidant property and cell proliferation and migration promoting ability, and can maintain metabolism and immune homeostasis of diabetic wounds, so that the Zn-DHM nano-enzyme can accelerate early healing of the diabetic wounds.

The invention relates to a culture medium and a culture method of persistent DC-CIK cells and application of the culture medium and the culture method, and belongs to the technical field of cell culture. The invention provides a culture medium for persistent DC-CIK cells, which takes a serum-free culture medium as a solvent and comprises a recombinant human granulocyte macrophage colony stimulating factor, a recombinant human interleukin-12, a recombinant human tumor necrosis factor-alpha, a recombinant human interferon-gamma, L-cysteine, glutathione, ginseng polysaccharide and streptomycin sulfate. According to the culture medium and the culture method of the persistent DC-CIK cells, proliferation and growth of the DC-CIK cells can be promoted, and very high tumor killing activity of the DC-CIK cells can be maintained for a long time.

The invention provides a nanoparticle compound for inhibiting bone and soft tissue infection as well as a preparation method and application thereof, and belongs to the technical field of medical biological materials. The nanoparticle compound is a gentiopicroside, sericin, hyaluronic acid and endolysin liposome nanoparticle, and a gentiopicroside, sericin, hyaluronic acid and endolysin co-delivery nano drug delivery system targeting the S100A4 site is designed based on the target S100A4 closely related to bone infection through Ai molecular docking and nano drug delivery technologies. The nanoparticle compound provided by the invention has better stability and bioavailability, and can be used for improving and relieving inflammatory response and increasing wound healing effect.

The invention discloses a livestock composite biological feed prepared from probiotics and a method thereof, and relates to the technical field of livestock feeds. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus niger BNCC147472, enterococcus faecalis BNCC186075, yarrowia lipolytica BNCC193899, nutrient substances, mineral trace elements, vitamins, amino acids and prebiotics. The compound biological feed for the livestock and poultry is prepared from the following raw materials: lactobacillus plantarum BNCC138404, bacillus coagulans BNCC132591, bacillus velezensis BNCC352076, bifidobacterium bifidum BNCC186493, aspergillus The compound biological feed fermented by adopting various probiotics can improve the antioxidant level of the broiler chicken, improve the health condition of intestinal tracts, increase the villus height and the villus-implicit ratio of the jejunum of the broiler chicken and reduce the occurrence of intestinal inflammation, so that the growth performance of the broiler chicken is promoted, and the immune performance of the body is improved.

The invention discloses multifunctional hydrogel for promoting healing of diabetic wounds and a preparation method of the multifunctional hydrogel. The multifunctional hydrogel is prepared from Ti3C2Tx-Ag, paeoniflorin (PF), a gel matrix oxidized konjac glucomannan (OKGM) solution and carboxymethyl chitosan (CMCS). The method comprises the following steps: mixing Ti3C2Tx powder with AgNO3 in deionized water, heating to boiling, adding a proper amount of a sodium citrate solution, reacting to obtain Ti3C2Tx-Ag (TA) powder, and mixing the powder with a paeoniflorin (PF) and oxidized konjac glucomannan (OKGM) solution and a carboxymethyl chitosan (CMCS) solution to prepare the KC-PF-TA hydrogel. The multifunctional hydrogel has excellent photo-thermal antibacterial performance, anti-inflammatory performance and angiogenesis promoting performance, shows a remarkable effect of promoting healing of diabetic wounds through the synergistic effect of Ti3C2Tx-Ag and paeoniflorin, and has good biocompatibility; the multifunctional nano-composite hydrogel disclosed by the invention has great application prospect and value in the field of wound repair materials.

The invention discloses bioactive polypeptide hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedicine. The antioxidant peptide MMP12 synthesized by the invention is used for removing ROS (reactive oxygen species) in an MI region, so that myocardial cells are protected; the invention relates to an angiogenic peptide KRX, which is used for treating myocardial ischemia during myocardial infarction. Experimental results show that the bioactive polypeptide hydrogel prepared by the invention not only has the functions of resisting oxidation and promoting angiogenesis, but also has obvious effects of resisting apoptosis, resisting inflammation and inhibiting myocardial fibrosis through the combination of the two polypeptides, and the effects can obviously improve the cardiac function, promote the repair of damaged myocardial tissues and improve the cardiac function. The ventricular wall remodeling is inhibited, and a feasible treatment scheme is provided for myocardial infarction treatment.

The invention discloses riboflavin photodynamic antibacterial hydrogel for a diabetes wound dressing as well as preparation and application of the riboflavin photodynamic antibacterial hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is prepared from carbomer 941, rhizoma bletillae polysaccharide and carboxymethyl chitosan through physical and chemical bi-crosslinking, glutaraldehyde and carboxymethyl chitosan are added to form dynamic imine bonds, and the stability and adaptability of the hydrogel are enhanced. Meanwhile, the photosensitizer riboflavin is introduced, so that the hydrogel is endowed with photodynamic sterilization characteristics, efficient antibiosis is realized, and rapid healing of diabetic wounds is promoted. The hydrogel has excellent wound environment adaptability and adhesion performance, and is suitable for complicated wound surfaces of diabetes mellitus. The successful research and development of the invention provide reference for the development of novel photodynamic wound dressings, have wide application prospects, provide better treatment choices for diabetics, and are helpful for accelerating wound healing, reducing infection risks and improving the life quality of patients.

The invention provides application of a PDLIM4 gene in preparation of a medicine for inhibiting non-small cell lung cancer proliferation, belongs to the technical field of cancer suppressor gene research, and finds that the PDLIM4 gene is highly expressed in non-small cell lung cancer tumors, and the prognosis of a patient with the highly expressed PDLIM4 gene is good; the proliferation of the non-small cell lung cancer cells overexpressed with the PDLIM4 gene is inhibited, and the proliferation of the non-small cell lung cancer cells knocked down with the PDLIM4 gene is accelerated, so that the reagent capable of realizing the overexpression of the PDLIM4 gene is confirmed to be of great significance for inhibiting the proliferation of the non-small cell lung cancer cells. The overexpression of the PDLIM4 gene in the non-small cell lung cancer cells can enhance the chemotherapy effect of the chemotherapy drug adriamycin, and can be used as a chemotherapy sensitizer. The BRD4 is an upstream regulatory factor for increasing the expression of the PDLIM4 gene in the non-small cell lung cancer, and can be used for promoting the expression of the PDLIM4 gene.

The invention belongs to the technical field of biological medicines, and particularly relates to an application of a Pucurbita pepo polypeptide Nacrein-LP20 in preparation of a medicine for promoting wound healing and a preparation method of the Pucurbita pepo polypeptide Nacrein-LP20. The amino acid sequence of the Hepu pearl polypeptide Nacrein-LP20 is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the present invention, the Pucurbita pepo active polypeptide Nacrein-LP20 has characteristics of strong DPPH free radical and hydroxyl free radical removing ability and excellent antioxidant activity, can significantly promote HaCaT cell proliferation in the high glucose environment, and can promote migration and repair of HaCaT cells in the high glucose environment; the traditional Chinese medicine composition has a remarkable healing promoting effect on full-cortical resection wound surfaces of normal rats and diabetic rats.

The invention provides an application of a recombinant XVII type humanized collagen or a composition containing the recombinant XVII type humanized collagen in preparation of a preparation for preventing and / or treating lupus erythematosus, which can overcome the defect that more adverse reactions are generated in treatment in the existing scheme.

The invention discloses an artificial antigen presenting cell for efficiently preparing a chimeric antigen receptor T cell (CAR-T) and a construction method of the artificial antigen presenting cell, and belongs to the field of cell therapy and immunotherapy, the artificial antigen presenting cell takes a K562 cell as a platform, an anti-CD3 single-chain antibody, an anti-CD28 single-chain antibody, a membrane binding type cell factor and a costimulatory ligand are stably expressed through genetic engineering, and the chimeric antigen receptor T cell (CAR-T) is obtained. And the local concentration of the anti-CD3 antibody is improved by adopting an HOTag3 tool. The multi-signal integration strategy obviously enhances the activation and proliferation capacity of the T cells, promotes the CAR-T cells to maintain a low differentiation state, and endows the CAR-T cells with excellent anti-tumor activity and durability. The artificial antigen presenting cell provides a novel efficient tool for the field of cellular immunotherapy, and is expected to promote development of more efficient and safer CAR-T therapy to cope with challenges of various malignant tumors.

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.