39 results about "Skin cell" patented technology

This invention discloses the application of homoserine as an aging regulator in the preparation of anti-aging skin compositions. As a multidimensional regulator of skin aging, homoserine integrates skin cell lifespan regulation, telomere protection, and oxidative protection, providing a multi-target synergistic mechanism that can comprehensively and effectively delay skin aging, reduce wrinkles and sagging, and enhance vitality. The application of homoserine in cosmetics or skin care products meets consumers' demand for precise anti-aging skincare.

The application discloses a postbiotic prepared from paracasei CCFM1294 and having the function of improving the HA content of skin cells, and belongs to the technical field of microorganisms. The postbiotic prepared from paracasei CCFM1294 has the function of promoting the synthesis of HA of a host: (1) significantly up-regulating the UGDH mRNA expression of skin cells; (2) significantly up-regulating the HAS1 mRNA expression of skin cells; (3) significantly up-regulating the HAS2 mRNA expression of skin cells; (4) significantly up-regulating the HAS3 mRNA expression of skin cells; (5) increasing the HA content of skin cells; therefore, the postbiotic prepared from paracasei CCFM1294 has the function of promoting the synthesis of HA of a host and preventing and / or repairing the symptoms, such as dry skin and aging, and has a great application prospect.

The present application provides a peptide having the formula (I) R1-Ser-Pro-X1-Gln-R2 or a cosmetically or pharmaceutically acceptable salt thereof, or a cosmetic or pharmaceutical composition thereof, and their use in the preparation of a cosmetic or pharmaceutical composition for treating, preventing or repairing skin aging or photoaging, promoting collagen production, increasing skin elasticity and / or skin firmness, promoting PER1 protein expression, regulating skin circadian rhythm and / or repairing damaged skin cells. The peptide of formula (I) regulates the expression of skin circadian cycle regulation genes and related proteins, regulates skin circadian rhythm and resynchronizes skin cell biological clock, enhances cell vitality, repairs damaged cells, thereby alleviating signs of skin aging, and can be used in the field of cosmetics or medicine to resist skin aging, and can also be used to prepare a PER1 protein activator.

This application relates to the field of cosmetic technology, specifically disclosing an anti-photoaging skincare composition suitable for sensitive skin and its application. This application uses camellia flower extract, butcher's broom root extract, saffron extract, and lactobacillus fermentation products to form an anti-photoaging skincare composition. These components work together and inextricably on the coherent biological axis of skin microecological regulation – enhanced bioelectrical signals – activation of mitochondrial function. By restoring the core energy metabolism of skin cells, it fundamentally reverses the photoaging process and simultaneously achieves comprehensive rejuvenation effects such as anti-wrinkle, firming, and brightening.

The application provides a synthesis method and application of silk fibroin-based melanin-like nanoparticles, and belongs to the technical field of nanoparticle preparations for biomedical use. After mixing silk fibroin nanoparticles, tyrosine and tyrosinase solution, silk fibroin-based melanin-like nanoparticles are obtained through one-pot copolymerization, and the enzyme concentration in the tyrosinase solution is 200-250 U / mL. The synthesis method has the advantages of simple operation, green environmental protection and the like, and the synthesized silk fibroin-based melanin-like nanoparticles have excellent antioxidant activity, ultraviolet protection and significant anti-skin cell photoaging.

The present invention relates to a composition comprising a combination of at least two cosmetic active agents, said combination of active agents preventing or reducing the loss of proteostasis, preventing or reducing micro-inflammatory processes, preventing cellular senescence, and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism in skin cells The invention also relates to the cosmetic use of such a composition for increasing the longevity of skin cells and skin tissues, for preventing or reducing the loss of proteostasis, preventing or reducing micro-inflammatory processes, preventing cellular senescence and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism, in skin cells. Finally, the invention relates to a nontherapeutic cosmetic process for caring for keratin materials comprising the topical application of such a composition.

The present application relates to the use of a substance that regulates BCL11A gene and / or its encoded product in the preparation of a drug for reversing skin photoaging, wherein the skin photoaging includes skin cell aging caused by sunlight, light, etc., and the skin cells include keratinocytes, dermal fibroblasts, melanocytes. The regulation is to up-regulate the expression of BCL11A gene and / or promote the production of its encoded product. Typical clinical manifestations of the skin photoaging include wrinkles, dry skin, loose skin, dilated capillaries, uneven skin pigmentation, and skin itching. The substance that regulates BCL11A gene and / or its encoded product includes a substance that regulates BCL11A gene DNA molecules and a substance that regulates the expression of BCL11A translation product protein molecules. The reversal of skin photoaging is manifested in reducing the proportion of cells positive for aging-related beta-galactosidase staining in skin cells and reducing the expression of aging-related protein p16 and DNA damage protein gammaH2AX.

This invention discloses lotus-derived LEA protein and its application in improving skin's resistance to dryness damage, belonging to the field of biotechnology and its application in skin care technology. The amino acid sequence of the LEA protein is shown in SEQ ID NO:1. This invention successfully constructed a recombinant expression vector containing a nucleic acid molecule encoding the LEA protein using genetic engineering methods, and transformed it into *E. coli* for efficient expression. After purification, the target protein was obtained. This LEA protein can significantly protect human immortalized keratinocytes against dryness damage. At a concentration of 100-200 μg / mL, it can restore cell viability under dryness stress to over 85%, with effects comparable to hyaluronic acid. This LEA protein has good biocompatibility and significant anti-dryness activity, and can be used to prepare skin care products that protect skin cells against dryness damage, moisturize, repair the skin barrier, or have anti-aging effects, showing broad application prospects.

The application belongs to the technical field of stem cell application, and provides a stem cell cytokine-containing composition, which comprises the following raw materials in mass percentage: stem cell cytokine concentrate 72-84%, trehalose 2-5%, mannitol 10-15%, antioxidant 3-5%, whitening component 1-7%, and the balance is water. The composition is safe to the skin and has no adverse reactions; can increase skin cell activity, promote skin cell metabolism, improve skin elasticity, and increase skin water content, so as to improve skin balance, achieve the effects of moisturizing, whitening, anti-aging and skin color lightening.

The present invention relates to the use, in particular cosmetic use, of azelaic acid, an organic or inorganic base salt thereof, a solvate thereof, or mixtures thereof, to increase the longevity of skin cells.

The application discloses an anti-wrinkle firming soothing efficacy component combination based on skin cell energy and application, and belongs to the technical field of cosmetics. The composition comprises EPHMER mitochondrial marine protective factors, adenosine silanol, DPHP ligament repair factors and AQUXYL five-dimensional barrier repair factors in specific proportions. The application takes mitochondrial energy metabolism as the core, constructs a "protection-energy supply-repair-barrier" multi-pathway synergistic system, and breaks through the single target limitation of traditional anti-aging components. The composition can specifically target mitochondria, significantly increase the ATP content of cells, efficiently promote collagen synthesis, strengthen the skin barrier and reduce irritation, and is especially suitable for sensitive skin anti-aging. Through cell, 3D skin model and human body test verification, the composition shows significant and mild effects in terms of anti-wrinkle, firming and soothing. The composition provided by the application can be widely applied to cosmetics such as essence and cream.

This invention discloses a bioactive composition with synergistic effects. The composition comprises a highly active stem cell extract prepared by a sequential pretreatment of hypoxia combined with heat shock, and a high-affinity monoclonal antibody P3F6 targeting human PTEN protein. The stem cell extract is rich in heat shock proteins and various growth factors; the monoclonal antibody P3F6 possesses specific light and heavy chain variable region sequences. Experiments have demonstrated that this composition exhibits significant synergistic effects in promoting skin cell proliferation, inhibiting apoptosis, enhancing skin barrier function, and promoting collagen synthesis, with effects far exceeding those of single components. This invention also provides the application of the composition in the preparation of skin repair, anti-aging cosmetics, and medical wound healing dressings.

This application provides a method for rejuvenating a population of cells, for example, skin cells, comprising introducing an effective amount of a recombinant reprogramming factor without liquid-liquid phase separation (LLPS) into the population of cells, for example, skin cells.

The application belongs to the technical field of cosmetic raw material preparation, and particularly relates to a tuberose flower wax as well as a preparation method and application thereof. The tuberose flower wax is obtained through a n-hexane extraction combined with a plant oil assisted extraction and a low-temperature freezing separation process, has natural light yellow color and rich characteristic fragrance, is high in safety, and has no toxic effect on skin cells in a relatively wide concentration range. Meanwhile, the flower wax has excellent antioxidant activity, can effectively scavenge DPPH free radicals, and significantly reduce the intracellular malondialdehyde level and oxidative damage. In addition, the flower wax prepared by the application also has high anti-inflammatory activity, and can inhibit the secretion of inflammatory factors. The process condition of the application is mild, the solvent can be recycled and used, and the product safety, natural activity reservation and industrial production feasibility are considered, and the prepared tuberose flower wax provides unique rheological property and touch feeling due to the wax structure and physical and chemical properties, and can be directly applied to cosmetics, skin care products and related functional products.

The present application relates to a kind of mRNA delivery system based on liposome with antioxidant efficacy, and functional cosmetic composition containing the system as active ingredient.The mRNA delivery system based on liposome and cosmetic composition of the present application can directly induce the production of antioxidant enzyme in skin cells, thereby providing sustained and effective antioxidant effect.This enables the mRNA delivery system to penetrate into the deep layer of skin, exerting antioxidant effect in epidermis and dermis, thereby enhancing the antioxidant system of skin cells themselves without relying on externally supplied antioxidants.In addition, it can also effectively prevent and improve the problems of skin aging, inflammation, pigmentation and the like caused by oxidative stress, and by applying mRNA technology to the field of cosmetics, it provides an innovative approach to lead a new paradigm for the cosmetic industry.

The present application relates to a plant extract-based liposoluble antioxidant and skin lightening composition and its skin care product. The plant extract-based liposoluble antioxidant and skin lightening composition comprises the following components by mass fraction: 5-50 of deep sea fish oil, 0.05-2 of tomato fruit extract & jojoba seed oil & squalane compound, and 0.05-1 of jojoba seed extract & olive fruit oil compound. The present application improves the skin lipid content through the synergistic effect of deep sea fish oil, tomato fruit extract & jojoba seed oil & squalane compound, and jojoba seed extract & olive fruit oil compound, resists oxidation in multiple dimensions, removes free radicals, reduces collagen loss, inhibits melanin production, increases skin hyaluronic acid content, enhances the ability of cells to resist oxidation, improves the glossiness and elasticity level of skin cells, and ultimately achieves the effects of antioxidant and overall whitening and skin lightening.

This application relates to a cyanobacteria extract with photothermal damage repair effects, its preparation method, and its application, belonging to the field of cosmetic raw material technology. The cyanobacteria extract provided in this application can be used to balance the inflammatory and repair signaling pathways of skin cells after photothermal damage, and can be used to prepare skin care products with soothing, anti-inflammatory, and barrier-repair-promoting effects. It can synergistically balance the pro-inflammatory damage signaling pathway TLR4 / NF-κB axis and the survival-promoting repair signaling pathway HSP90 / PI3K-Akt axis simultaneously activated in skin cells after photothermal damage, targeting multiple points. This can more effectively control excessive inflammation, reduce secondary damage, and enhance the cell's self-repair ability. It is suitable for various scenarios such as sunburn, post-laser surgery, and thermal environment damage, and has significant technical advantages compared to existing technologies, with broad application prospects and huge market value.

The present invention relates to a liposome-based mRNA delivery system having an effect of improving skin elasticity and improving wrinkles, and use thereof. The liposome-based mRNA delivery system according to the present invention provides an excellent effect in improving skin elasticity and wrinkles, and can maximize the efficacy of preventing skin aging and regeneration. In addition, the system of the present invention stably encapsulates and effectively delivers mRNA to skin cells, providing higher efficiency and safety than existing methods. In other words, in the present invention, synthetic mRNA is wrapped in a liposome-based nanoparticle and stably delivered into skin cells, thereby inducing collagen synthesis in skin fibroblasts and improving skin structure.

Cosmetic composition for skin cell longevity The present invention relates to a composition comprising a combination of at least two cosmetic active agents, said combination of active agents preventing epigenetic modifications, preventing or reducing the loss of proteostasis, preventing or reducing micro- inflammatory processes, and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism in skin cells The invention also relates to the cosmetic use of such a composition for increasing the longevity of skin cells and skin tissues, preventing epigenetic modifications, preventing or reducing the loss of proteostasis, preventing or reducing micro-inflammatory processes, and preventing or reducing mitochondrial dysfunction and / or stimulating mitochondrial energy metabolism, in skin cells. Finally, the invention relates to a nontherapeutic cosmetic process for caring for keratin materials comprising the topical application of such a composition.

This invention relates to the field of biotechnology and discloses a stem cell co-expressing miR-449a-5p and miR-30c-5p. The stem cell is constructed by cloning a biomimetic polycistronic expression cassette containing miR-449a-5p and miR-30c-5p into a lentiviral vector and infecting the stem cell, enabling stable and synergistic expression of these two miRNAs. The exosomes (miRNAs-Exos) secreted by these cells specifically enrich miR-449a-5p and miR-30c-5p; after local administration, the exosomes are taken up by skin cells, and the two miRNAs synergistically inhibit the expression of the aging-associated secretory phenotype (SASP) core factors CCL2 and PAI-1, downregulate matrix metalloproteinases MMP-1 / MMP-3, reduce collagen degradation, improve the dermal inflammatory microenvironment, and thereby promote collagen synthesis and skin barrier repair.

This invention belongs to the field of cosmetic composition technology, specifically relating to an essential oil composition with repairing and anti-aging effects and its application. The essential oil composition consists of carrot seed oil, white chamomile flower oil, and unsaponifiables from shea butter. This composition effectively promotes the release of endorphins from skin cells, helping to relieve skin stress; simultaneously, it significantly inhibits excessive cortisol secretion under stress, alleviating skin stress responses at their source. This composition exhibits excellent barrier repair capabilities, promoting keratinocyte migration and scratch healing, upregulating the expression of barrier-related genes, and effectively scavenging UV-induced reactive oxygen species, reducing oxidative damage. Furthermore, this composition significantly promotes the expression of type I and type III collagen, exerting repairing and anti-aging effects from multiple perspectives.

This invention discloses a wearable colorimetric ultraviolet (UV) dose sensor based on gold nanoparticles. It utilizes 2-hydroxy-2-methyl-1-phenyl-1-propanone (HMPP) as a photosensitizer, which generates free radicals under UV irradiation, thereby inducing the aggregation of gold nanoparticles (AuNPs). This causes the solution color to change from red to purple, and the degree of color change exhibits a strong linear relationship with the UV radiation dose. By injecting the mixed reaction solution into a flexible, transparent polydimethylsiloxane (PDMS) substrate, a wearable colorimetric UV dose sensor patch that can be attached to the skin is prepared. Combined with RGB red channel analysis of a smartphone, real-time quantitative monitoring of UV dose can be achieved. A bio-effect colorimetric card constructed using an in vitro cell model can also visually assess the potential damage of UV radiation to skin cells. This sensor is low-cost, portable, and visually appealing, making it suitable for personal health protection, outdoor activities, and occupational safety.

This invention discloses a single-domain antibody targeting phosphatidylserine, an engineered exosome based on this antibody, its preparation method, and its application in the preparation of skin repair products. The single-domain antibody has the amino acid sequence shown in SEQ ID NO:1. This invention further provides an engineered exosome whose surface is covalently linked to the single-domain antibody, and whose interior is enriched with mRNA encoding GPX4. This exosome is derived from human placental mesenchymal stem cells treated with a specific sequential induction protocol that can mimic the damaged microenvironment and upregulate the expression of specific functional molecules. The constructed engineered exosome can actively recognize and target the delivery of therapeutic molecules to damaged skin cells with everted phosphatidylserine, thereby effectively inhibiting ferroptosis, repairing UV-induced photoaging damage, and enhancing skin barrier function, providing a new technical solution to address the problem of insufficient targeting in existing exosome therapies.

The application discloses an anti-aging multi-type collagen composition and a preparation method and application thereof, and belongs to the field of food technology. The technical problem to be solved is that the expression level of human skin cell MMP-1 and MMP-9 genes is increased, the generation of glycation end products AGEs is increased, and the activity of superoxide dismutase SOD is decreased due to ultraviolet irradiation. The technical solution point is that the anti-aging multi-type collagen composition comprises 50-500 parts of type I collagen peptide, 10-200 parts of type II collagen peptide and 10-300 parts of type III collagen peptide in terms of weight.

The present invention relates to a composition comprising a combination of at least 2 cosmetic active agents, said combination of active agents directly or indirectly protecting the stability of DNA, preventing epigenetic modifications, in particular those affecting the expression of the genes involved in the maintenance of skin integrity, preventing or reducing microinflammatory processes, and preventing or reducing mitochondrial dysfunctions and / or stimulating mitochondrial energy metabolism, in the cells of the skin It also relates to the cosmetic use of such a composition for increasing the longevity of skin cells and skin tissues, for directly or indirectly protecting the stability of DNA, preventing epigenetic modifications, preventing or reducing microinflammatory processes, and preventing or reducing mitochondrial dysfunctions and / or stimulating mitochondrial energy metabolism, in the cells of the skin. Lastly, it relates to a non-therapeutic cosmetic process for caring for keratin materials, comprising the topical application of such a composition.

Provided is a three-dimensional culture of immortalized skin cells, comprising a plate-like substrate and a culture layered product of immortalized skin cells adhering to the surface of the plate-like substrate, in which the superficial layer of the culture layered product is composed of a stratum corneum. The three-dimensional culture can be produced as follows: immortalized skin cells are seeded on a top surface of a culture medium-impregnated plate-like substrate, and three-dimensional culture is performed while applying stress on a superficial layer of an immortalized skin cell layer formed by layering of the immortalized skin cells. The three-dimensional culture can be used to evaluate the sensitization of a test substance.

A skin treatment system that uses ultrasound devices, and more particularly, handheld devices with an ultrasound transducer used in combination with a continuous flow of serum carrying acoustically responsive media wherein acoustic energy transmitted from the transducer causes dimensional changes in the acoustically responsive media to create acoustic pressure peaks capable of loosening paracellular junctions in the skin to enhance penetration of serum into the skin.

The application relates to a PDRN-loaded collagen microgel, a preparation method and application thereof, and belongs to the technical field of biomaterials and preparations. In order to solve the problems of low transdermal efficiency and poor preparation stability of recombinant collagen in skin repair applications, a PDRN-loaded collagen microgel is provided, a microgel carrier is constructed based on an enzymatic crosslinking strategy, a deep eutectic solvent / buffer system is combined with ultrasonic treatment to dissolve rhCol III and CMG, the solubility and mixing uniformity of the two are significantly enhanced, papain catalytic crosslinking is used to construct a stable covalent network and load PDRN, the microgel is high in safety, has good slow-release performance, is excellent in stability, can effectively promote skin cell migration, proliferation and barrier repair-related gene expression, and can significantly improve wound healing effect, and provides an innovative solution for developing a new type of efficient and safe cosmetic or external active ingredient delivery system for skin repair.

This invention discloses a 3D cell sphere for treating skin defects, its preparation method, and its applications, belonging to the field of biomedical materials. The 3D cell sphere is formed by the self-assembly of keratinocytes (KCs) and cells capable of secreting extracellular matrix, possessing a biomimetic hierarchical structure similar to natural skin, filled with extracellular matrix, and the KCs can form functional epidermal-associated structures. The preparation method involves obtaining EKCs through directed induction differentiation of pluripotent stem cells, which are then spontaneously assembled with cells capable of secreting extracellular matrix to form 3D cell spheres. The 3D cell sphere exhibits excellent biocompatibility, a simple preparation process, and can be mass-produced, with convenient operation. It is suitable for repairing various acute and chronic skin defects such as large-area thermal burns, diabetic foot ulcers, and pressure ulcers, effectively accelerating wound healing and improving the quality of epidermal regeneration. It addresses the pain points of traditional treatments, such as scarcity of skin cells, complex procedures, and poor efficacy, providing a safe and efficient novel cell therapy for skin defect repair.