214 results about "Skin cell" patented technology

The invention discloses a recombinant XVII type collagen engineering bacterium as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering, and the recombinant XVII type collagen engineering bacterium is realized through three steps of designing and optimizing collagen, improving translation through codon optimization and selecting a vector for cloning. By optimizing the amino acid sequence and the codon, the expression efficiency and the stability of the recombinant XVII type collagen in engineering bacteria are improved. The optimization not only improves the production efficiency of the protein, but also reduces the production cost. The recombinant XVII type collagen has potential in the medical field, especially in the fields of skin repair, wound healing, aging resistance and the like. Meanwhile, in the fields of beauty and biological materials, collagen is widely applied to products such as masks and skin care products as a basic component, and can promote skin cell regeneration and enhance skin elasticity and repair capacity.

The invention discloses a plant lactobacillus capable of metabolizing dendrobium officinale components to generate various active small molecules and enhancing collagen synthesis promotion of skin cells, and belongs to the technical field of microorganisms. The lactobacillus plantarum (Lactobacillus plantarum) CCFM1496 obtained by screening can be used for fermenting the dendrobium officinale to generate a plurality of active substances and regulating and controlling the synthesis of skin collagen factors, and LOR (Lyclin) shows that the fermentation liquor of the dendrobium officinale fermented by the lactobacillus plantarum (Lactobacillus plantarum) CCFM1496 has a huge application prospect in the preparation of products for preventing or treating skin quality.

The present invention relates to a functional cosmetic composition comprising plant exosomes derived from ginseng as an active ingredient and a functional cosmetic containing the same. Ginseng exosomes have a bilipid layer structure and are rich in ginsenosides Rb1 and Rg1, which influence skin improvement and whitening. It has been proven that these powerful functional materials, ginsenosides Rb1 and Rg1, are absorbed in large quantities through ginseng exosomes into skin cells, enhance the synthesis of collagen and hyaluronic acid in skin cells, and have the efficacy of inhibiting melanin synthesis. Therefore, the ginseng exosomes rich in ginsenosides Rb1 and Rg1, presented in the present invention, can be beneficially used in functional cosmetic compositions for skin aging prevention, skin whitening, wrinkle improvement and skin cell regeneration.

The invention discloses a PDRN nano-liposome preparation method, which comprises: S1, taking hydrogenated lecithin, polyglycerol-10 myristate, stearoyl glutamate and glycerin, heating, and uniformly mixing to obtain an oil phase liquid; s2, adding macromolecular PDRN, micromolecular PDRN and hydrolyzed sodium hyaluronate into water, heating and uniformly mixing to obtain a water-phase liquid; s3, adding the water-phase liquid into the oil-phase liquid in a stirring state, and continuously stirring to obtain crude lipidosome; s4, carrying out microjet treatment on the crude liposome under the pressure of less than or equal to 1000bar to obtain the PDRN nano-liposome; wherein the step S1 and the step S2 do not have sequence requirements. The invention also provides the prepared PDRN nano-liposome. The invention further provides the PDRN nano-liposome. The PDRN nano-liposome provided by the invention is high in stability, can rapidly permeate, synergistically enhances the skin cell repairing and anti-aging effects, and is simple in preparation process.

The invention discloses a poly-deoxyribonucleotide salt and a preparation method and application thereof, the content of the poly-deoxyribonucleotide salt is greater than 95%, the protein content is less than 1.0%, and after high-pressure moist heat sterilization, the dynamic viscosity reduction amount does not exceed 25%, and the elastic index G'reduction amount does not exceed 30%. The polydeoxyribonucleotide salt disclosed by the invention is simple in preparation process, short in production period, low in cost and suitable for large-scale industrial production; and the preparation process is mild, few impurities are introduced, the product structure is less damaged, and the preparation method is environment-friendly. The polydeoxyribonucleotide salt disclosed by the invention has good physical support performance, has the effects of promoting skin cell repair, resisting skin sensitivity, whitening, resisting inflammation, preventing alopecia and growing hair, and has a wide market prospect.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention provides a preparation method of human collagen and an application of the human collagen in anti-aging. The amino acid sequence of the human collagen is as shown in SEQ ID NO: 1; based on a natural collagen structure, a fusion sequence structure is provided, and the proportion of individual amino acids is adjusted; the collagen can promote skin cell growth and hyaluronic acid secretion; skin aging caused by ultraviolet radiation is resisted, inflammatory factor expression is inhibited, and oxidative stress is resisted.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

A skin care composition includes a plurality of agents that include at least one retinol derivative that may comprise, for example, hydroxypinacolone retinoate, and at least one bark extract that may comprise, for example, Eperua falcata bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may also include hydroxy acid, for example, comprising lactic acid. The composition may confer one or a combination of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

Microfluidic assay devices that support multi-layer cellular matrices (e.g., ex-vivo skin cell matrices) and associated methods of fabrication and use are provided. A holder including a microfluidic chamber includes multiple sidewall ports disposed at different levels, multiple inlet ports in communication with different sidewall ports, and a liquid-permeable membrane bounding a chamber bottom. Stratified layers of cell-containing material may be formed in the chamber by sequential steps of introducing cell-hydrogel precursor material through a sidewall port and crosslinking the same, then repeating the steps for each sidewall port, to form cell-containing material layers in contact with one another. A cover with distribution channel(s) is provided below the liquid-permeable membrane. A continuous upper extension of the chamber may be open from above to provide interface(s) for receiving analytes and / or for environmental interaction. A holder may removably receive multiple assay devices.

The present application relates to the use of recombinant collagen in the preparation of a preparation for increasing the expression of skin anti-wrinkle related genes, wherein the amino acid sequence of the recombinant collagen comprises the sequence shown in SEQ ID NO. 1. The present application detects the expression of a series of skin anti-wrinkle related genes by transcriptomics, and proves that the recombinant collagen provided by the present application can promote the expression of skin cell anti-wrinkle genes, and can be used for the development and application of various cosmetic products, and endows the cosmetic products with the efficacy and effect of promoting skin anti-wrinkle ability. The above-mentioned genes can also be used as a reference for evaluating the skin anti-wrinkle ability of other raw materials or cosmetics in the future.

The invention provides a marker for diagnosing and treating radiodermatitis and application of the marker. The relation between SENP5 and radiodermatitis is researched for the first time, it is found that SENP5 is in positive correlation with occurrence and development of radiodermatitis, an SENP5 overexpression group shows better radiation resistance, skin cells are protected, and damage caused by radiation is relieved. The SENP5 is mainly used for promoting DNA damage repair by mediating an HR repair pathway. Besides, the SENP5 knocked-down cell line is successfully constructed by designing shRNA for specifically knocking down the SENP5 expression level, and it is proved that after SENP5 knocked-down skin cells are irradiated by 60Co rays, the cell survival level is lowered, the homologous recombination repair pathway is obviously inhibited, the NF-kappa B pathway is obviously activated, and the inflammation level is improved. Therefore, SENP5 is represented as an anti-radiation gene in skin cells, and SENP5 can be used as a biomarker of radiodermatitis for early diagnosis of radiodermatitis and targeted preparation of drugs for radiodermatitis.

A skin care composition includes a plurality of agents that include at least one retinol derivative and at least one bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may include at least one retinol derivative comprising hydroxypinacolone retinoate and at least one bark extract which may include at least one bark extract comprising Eperua falcata bark extract. The plurality of agents may also include at least one hydroxy acid, for example, comprising lactic acid. The composition confers one or more of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, or a combination thereof. Application the plurality of agents confers to skin cells and tissues an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

The present application relates to a kind of long stem ilex ginseng new use in skin care product and active composition and skin care product, product is emulsion, cream, water agent, spray, gel and other skin care products, the components of emulsion include: active composition, humectant, emollient, emulsifier, thickening agent and deionized water, the mass percentage content of active composition in skin care product is 0.005%-0.1%, active composition includes long stem ilex ginseng and wujin ginseng, the mass ratio of long stem ilex ginseng and wujin ginseng is 5:1-1:1, long stem ilex ginseng has at least one of the following effects: (1) UVB light damage pre-protection to skin;(2) after UVB light damage repair to skin;(3) up-regulate the trichohyalin gene expression in skin cell.The long stem ilex ginseng of the present application has the effects of UVB light damage pre-protection to skin, after UVB light damage repair to skin and up-regulating the trichohyalin gene expression in skin cell to repair skin barrier.

The invention relates to a saccharomyces cerevisiae cloned strain and application thereof. Specifically, the invention provides a saccharomyces cerevisiae clone strain A154 clone, a culture, a lysate or an extract and a preparation thereof, a yeast active substance derivative prepared from the saccharomyces cerevisiae clone strain A154 clone, and application of the saccharomyces cerevisiae clone strain A154 clone in preparation of a product for improving the irradiation resistance of skin cells.

This invention discloses an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in the indicated mass percentages: 22%~28% helichrysum essential oil, 22%~28% sweet basil essential oil, 22%~28% eucalyptol rosemary essential oil, and 22%~28% true lavender essential oil. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientifically formulated compound. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products.

This invention relates to a skin cell dispersion for autologous treatment of skin wounds. Specifically, this invention provides a skin cell dispersion containing skin cells, epidermal growth factor, and transforming growth factor-β1. The skin cell dispersion of this invention exhibits excellent therapeutic effects on skin wounds.

This invention discloses the application of homoserine as an aging regulator in the preparation of anti-aging skin compositions. As a multidimensional regulator of skin aging, homoserine integrates skin cell lifespan regulation, telomere protection, and oxidative protection, providing a multi-target synergistic mechanism that can comprehensively and effectively delay skin aging, reduce wrinkles and sagging, and enhance vitality. The application of homoserine in cosmetics or skin care products meets consumers' demand for precise anti-aging skincare.

The present application relates to a kind of anti-ultraviolet damage composition and its application in sunscreen product, the anti-ultraviolet damage composition includes the following raw materials by weight parts: 5-8 parts of daphne giraldii leaf extract, 3-7 parts of green algae extract, 1-4 parts of dogwood extract, 8-10 parts of sea buckthorn extract, 2.5-4 parts of avocado butter and 5-8 parts of tea seed oil, each raw material has synergistic effect, by neutralizing the free radical induced by ultraviolet, reduce oxidative stress, protect skin cells from damage;Absorb ultraviolet, reduce the penetration of ultraviolet through skin, thereby reducing the direct damage of ultraviolet to skin;Regulate tyrosinase activity or block the melanin synthesis pathway, reduce the accumulation of melanin, help to prevent and improve the efficacy of anti-ultraviolet damage such as pigmentation, dull and other ways.

The invention relates to a preparation method of a small nucleic acid combined microneedle and application of the small nucleic acid combined microneedle to skin light injury repair, and particularly discloses a microneedle composition which comprises a compound of SNAP25 specific siRNA and nano-selenium and hyaluronic acid, the nucleotide sequence of a positive-sense strand of the siRNA is shown as SEQ ID NO.1, and the nucleotide sequence of an antisense strand of the siRNA is shown as SEQ ID NO.2. The invention further discloses a preparation method of the microneedle composition. The microneedle composition provided by the invention can realize stable delivery of siRNA, inhibit UV-induced skin photoaging related gene expression, increase repair of skin extracellular matrix and significantly improve skin light damage, and is suitable for clinical transformation application.

The present application relates to a kind of anti-aging composition for promoting intercellular communication of skin cell and its application, the composition includes the following components: camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside, the weight ratio of the camellia japonica flower extract, bacillus fermentation product, european linden extract and safflower glucoside is (1-10):(1-8):(1-6):(0.1-1).The anti-aging composition of the present application can promote the activity of skin communication cell, strengthen intercellular communication and interaction, fully exert the function of cell biology, achieve the effect of anti-aging;And inhibit the damage of cortisol to skin barrier, achieve the effect of repair and emotional stress relief, synergistic effect between components, can achieve the best wrinkle, firming, smoothing and repair effect.

The invention discloses an application of an engineered mRNA (messenger Ribonucleic Acid) medicine for coding COL3A1 protein in resisting skin photoaging. According to the method, accurate rational design and optimization are carried out on the 5'untranslated region (UTR) of the hCOL3A1 mRNA, so that the translation efficiency and the protein yield of the hCOL3A1 mRNA are greatly improved, the expression level of the hCOL3A1 target protein far beyond the conventional sequence in vivo and in vitro is realized, and the type III collagen is efficiently and endogenously synthesized in skin cells. Experimental results show that the engineered mRNA can effectively reduce the skin oxidative stress level, inhibit cell aging and promote endogenous synthesis and deposition of corium layer collagen, so that the barrier function of photoaged skin is remarkably improved, the dermis thickness is increased, the tissue structure is repaired, and systemic toxicity or immune side effects are not observed. The invention provides a novel strategy with high efficiency and safety for resisting skin photoaging.

Disclosed are a cell sampling unit and a cell sampling tool, the cell sampling unit comprising: a plurality of microcolumns for contacting a sample and obtaining cells from a surface of the sample by friction; and a base layer comprising a front side for supporting the plurality of microcolumns and acquired cells, in which one end of each microcolumn is fixed to or extends from the front side of the base layer, and the other end is a free end comprising a contact surface having a frictional force; wherein each microcolumn has a diameter of about 60-140 [mu] m and a height of about 60-140 [mu] m; wherein the plurality of microcolumns are configured in an array arrangement; the microcolumns are distributed at a density between about 1000 to 1400 microcolumns / cm2. Other example embodiments are described herein. In certain embodiments, the cell sampling unit is an easy to use, convenient and reliable skin cell sampling for male HPV screening.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging cell metabolism accelerating cosmetic and a preparation method thereof, and the technical scheme is that the anti-aging cell metabolism accelerating cosmetic comprises the following components: 0.0001%-0.005% of a cell growth factor compound, 0.0001%-0.003% of a small molecule peptide compound, 0.1%-5% of a plant stem cell extract, 1%-10% of grease, and the balance of water. 1%-10% of a surfactant, 1%-15% of a humectant, 0.1%-1% of an antioxidant, 0.1%-3% of a pH regulator, 0.05%-0.2% of a preservative and the balance of deionized water; epidermal and dermal layer cell proliferation is promoted through the cell growth factor, cell metabolism is regulated and controlled and collagen synthesis is promoted through the small molecule peptide, the plant stem cell extract is rich in polyphenol active ingredients and can activate the activity of skin endogenous stem cells, and the three components have a synergistic effect through different action pathways; the metabolism rate of skin cells and the anti-aging effect are obviously improved.

The present invention relates to a composition for improving skin condition, the composition comprising 15-HEPE, 5,15-HETE, or a combination thereof. The composition comprising 15-HEPE, 5,15-HETE, or a combination thereof according to one aspect alleviates skin inflammation or irritation, and exhibits skin barrier-strengthening, wrinkle-reducing, moisturizing, and skin cell-regenerating effects, and thus may be used as a cosmetic or a skin external preparation and the like for improving skin condition.

The application discloses a tremella aurantialba polysaccharide degradation product, which is prepared by degrading tremella aurantialba polysaccharide by a method of combining H2O2 and vitamin C and selecting degradation products with a molecular weight greater than 100 k Da and a molecular weight of 1 k Da to 100 k Da. Effective degradation of the tremella aurantialba polysaccharide can greatly improve the bioavailability of the tremella aurantialba polysaccharide, and the tremella aurantialba polysaccharide degradation product I (E-TAP I) and the tremella aurantialba polysaccharide degradation product II (E-TAP II) have good application prospects in skin thermal injury repair.

This invention belongs to the field of skin-like tissue preparation technology, and relates to a method for constructing ordered skin-like tissue with magnetic field assistance. The method involves directional cryo-casting to prepare a layered ordered hydrogel, providing skin-like seed cells, and culturing and expanding these cells in large quantities using a complete culture medium to obtain skin-like cells. The cultured skin-like cells are then co-cultured with iron oxide nanoparticles to prepare magnetized skin-like cells. These magnetized skin-like cells are then co-cultured with the layered ordered hydrogel, and magnetic fields are applied to both sides to induce the magnetized skin-like cells to enter the layered ordered hydrogel, resulting in a hydrogel scaffold. After washing the obtained hydrogel scaffold, it is transferred to a complete culture medium, and keratinocytes are seeded on the surface of the hydrogel scaffold and cultured to prepare an ordered skin-like tissue. This invention can effectively regulate the migration and arrangement of fibroblasts, providing a physical basis for the arrangement of the extracellular matrix, especially collagen.

The application discloses a postbiotic prepared from paracasei CCFM1294 and having the function of improving the HA content of skin cells, and belongs to the technical field of microorganisms. The postbiotic prepared from paracasei CCFM1294 has the function of promoting the synthesis of HA of a host: (1) significantly up-regulating the UGDH mRNA expression of skin cells; (2) significantly up-regulating the HAS1 mRNA expression of skin cells; (3) significantly up-regulating the HAS2 mRNA expression of skin cells; (4) significantly up-regulating the HAS3 mRNA expression of skin cells; (5) increasing the HA content of skin cells; therefore, the postbiotic prepared from paracasei CCFM1294 has the function of promoting the synthesis of HA of a host and preventing and / or repairing the symptoms, such as dry skin and aging, and has a great application prospect.

The utility model relates to the technical field of cell culture, and discloses a biological reaction vessel for in-vitro culture of tissue engineering skin cells, which comprises a liquid storage tank, the top of the liquid storage tank is fixedly connected with a culture bin through supporting legs, and the culture bin is used for culturing cell samples. A plurality of placing grooves are formed in a supporting ring arranged at the top of a culture bin to place polycarbonate films, so that multiple groups of cells can be conveniently cultured by adopting the same culture solution, and floating rods are arranged on the outer walls of the polycarbonate films, so that the polycarbonate films can float on the liquid surface, and the culture efficiency is improved. In order to facilitate the culture mode of skin cells on a polycarbonate film, gas-liquid interface culture is mainly adopted, the gas-liquid interface culture method can realize the multi-layer growth of the cells, the cells in contact with the liquid level of a culture medium have growth and reproduction capabilities in the culture process, and only the cells in contact with air are keratinized, so that the actual growth environment of the skin cells can be simulated, and the skin cells can be effectively cultured. And the cultured data is more referential.