293 results about "Skin cell" patented technology

The invention discloses a recombinant XVII type collagen engineering bacterium as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering, and the recombinant XVII type collagen engineering bacterium is realized through three steps of designing and optimizing collagen, improving translation through codon optimization and selecting a vector for cloning. By optimizing the amino acid sequence and the codon, the expression efficiency and the stability of the recombinant XVII type collagen in engineering bacteria are improved. The optimization not only improves the production efficiency of the protein, but also reduces the production cost. The recombinant XVII type collagen has potential in the medical field, especially in the fields of skin repair, wound healing, aging resistance and the like. Meanwhile, in the fields of beauty and biological materials, collagen is widely applied to products such as masks and skin care products as a basic component, and can promote skin cell regeneration and enhance skin elasticity and repair capacity.

The invention discloses a plant lactobacillus capable of metabolizing dendrobium officinale components to generate various active small molecules and enhancing collagen synthesis promotion of skin cells, and belongs to the technical field of microorganisms. The lactobacillus plantarum (Lactobacillus plantarum) CCFM1496 obtained by screening can be used for fermenting the dendrobium officinale to generate a plurality of active substances and regulating and controlling the synthesis of skin collagen factors, and LOR (Lyclin) shows that the fermentation liquor of the dendrobium officinale fermented by the lactobacillus plantarum (Lactobacillus plantarum) CCFM1496 has a huge application prospect in the preparation of products for preventing or treating skin quality.

The invention relates to an anti-ultraviolet-damage composition and application thereof in sunscreen products, and the anti-ultraviolet-damage composition is prepared from the following raw materials in parts by weight: 5-8 parts of oxhide tea leaf extract, 3-7 parts of green alga extract, 1-4 parts of dogwood extract, 8-10 parts of sea-buckthorn extract, 2.5-4 parts of butyrospermum parkii butter and 5-8 parts of camellia oleosa seed oil. All the raw materials have a synergistic effect, and free radicals generated by ultraviolet induction are neutralized, so that oxidative stress is relieved, and skin cells are protected from being damaged; ultraviolet rays are absorbed and are prevented from penetrating through the skin, so that direct damage of the ultraviolet rays to the skin is reduced; the activity of tyrosinase is regulated or a melanin synthesis pathway is blocked, the accumulation of melanin is reduced, and the effects of preventing and improving stains, darkness and the like are achieved.

The present invention relates to a functional cosmetic composition comprising plant exosomes derived from ginseng as an active ingredient and a functional cosmetic containing the same. Ginseng exosomes have a bilipid layer structure and are rich in ginsenosides Rb1 and Rg1, which influence skin improvement and whitening. It has been proven that these powerful functional materials, ginsenosides Rb1 and Rg1, are absorbed in large quantities through ginseng exosomes into skin cells, enhance the synthesis of collagen and hyaluronic acid in skin cells, and have the efficacy of inhibiting melanin synthesis. Therefore, the ginseng exosomes rich in ginsenosides Rb1 and Rg1, presented in the present invention, can be beneficially used in functional cosmetic compositions for skin aging prevention, skin whitening, wrinkle improvement and skin cell regeneration.

The invention relates to an anti-aging composition for promoting skin intercellular communication and application thereof, and the composition comprises the following components: a camellia nitidissima flower extract, a bacillus fermentation product, an European basswood extract and safflower glucoside, the weight ratio of the golden camellia flower extract to the bacillus fermentation product to the European basswood extract to the safflower glucoside is (1-10): (1-8): (1-6): (0.1-1). The anti-aging composition provided by the invention can promote the activity of skin communication cells, enhance intercellular communication and interaction, give full play to the biological function of cells, and achieve the anti-aging effect; the composition has the effects of removing wrinkles, tightening, smoothing and repairing, can inhibit damage of cortisol to skin barriers, achieves the effects of repairing and relieving mood pressure, and can achieve the optimal effects of removing wrinkles, tightening, smoothing and repairing due to mutual synergism of the components.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

The invention discloses a PDRN nano-liposome preparation method, which comprises: S1, taking hydrogenated lecithin, polyglycerol-10 myristate, stearoyl glutamate and glycerin, heating, and uniformly mixing to obtain an oil phase liquid; s2, adding macromolecular PDRN, micromolecular PDRN and hydrolyzed sodium hyaluronate into water, heating and uniformly mixing to obtain a water-phase liquid; s3, adding the water-phase liquid into the oil-phase liquid in a stirring state, and continuously stirring to obtain crude lipidosome; s4, carrying out microjet treatment on the crude liposome under the pressure of less than or equal to 1000bar to obtain the PDRN nano-liposome; wherein the step S1 and the step S2 do not have sequence requirements. The invention also provides the prepared PDRN nano-liposome. The invention further provides the PDRN nano-liposome. The PDRN nano-liposome provided by the invention is high in stability, can rapidly permeate, synergistically enhances the skin cell repairing and anti-aging effects, and is simple in preparation process.

The invention discloses a poly-deoxyribonucleotide salt and a preparation method and application thereof, the content of the poly-deoxyribonucleotide salt is greater than 95%, the protein content is less than 1.0%, and after high-pressure moist heat sterilization, the dynamic viscosity reduction amount does not exceed 25%, and the elastic index G'reduction amount does not exceed 30%. The polydeoxyribonucleotide salt disclosed by the invention is simple in preparation process, short in production period, low in cost and suitable for large-scale industrial production; and the preparation process is mild, few impurities are introduced, the product structure is less damaged, and the preparation method is environment-friendly. The polydeoxyribonucleotide salt disclosed by the invention has good physical support performance, has the effects of promoting skin cell repair, resisting skin sensitivity, whitening, resisting inflammation, preventing alopecia and growing hair, and has a wide market prospect.

The invention discloses a composition for reversing skin cell aging and application thereof, and relates to the technical field of cosmetics. The invention provides a composition. The composition comprises a component A and a component B, the component A comprises a camellia nitidissima extract, a camellia yunnanensis flower extract and a catechu extract; and the component B is oat beta-glucan. When the camellia nitidissima extract, the camellia yunnanensis flower extract and the catechu extract cooperate with the oat beta-glucan, small-molecule terpenoids and the like in the extracts can be used as natural penetration enhancers to form molecular channels with the oat beta-glucan; meanwhile, the components are synergistic, so that the removal of senescent cells can be enhanced, the cell regeneration is further enhanced, the cutin is effectively removed, the skin is brightened, and the mild anti-aging and brightening effects on the sensitive skin are achieved.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention provides a preparation method of human collagen and an application of the human collagen in anti-aging. The amino acid sequence of the human collagen is as shown in SEQ ID NO: 1; based on a natural collagen structure, a fusion sequence structure is provided, and the proportion of individual amino acids is adjusted; the collagen can promote skin cell growth and hyaluronic acid secretion; skin aging caused by ultraviolet radiation is resisted, inflammatory factor expression is inhibited, and oxidative stress is resisted.

The invention discloses a method for screening an active matter for protecting skin cell telomeres, and relates to the technical field of biotechnology and cosmetic development, and the method is technically characterized by comprising the following steps: a) providing stem cells derived from an exosome which carries at least one microRNA for regulating and controlling a telomere protection factor CTC1; b) acting a candidate active matter on the stem cells, and separating to obtain a treated exosome; the technical effects are as follows: the combination of miR-150-5p and miR-181c is delivered through the exosome, the dual-miRNA coordinated regulation is realized, the limitation of the traditional single-target detection is broken through, the CD63 / CD81 protein on the membrane surface of the exosome can be specifically combined with the skin cell membrane, and the Franz diffusion experiment verifies that the micro-fluidic chip integrates the collagen scaffold and the dynamic shear force.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

A skin care composition includes a plurality of agents that include at least one retinol derivative that may comprise, for example, hydroxypinacolone retinoate, and at least one bark extract that may comprise, for example, Eperua falcata bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may also include hydroxy acid, for example, comprising lactic acid. The composition may confer one or a combination of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

Microfluidic assay devices that support multi-layer cellular matrices (e.g., ex-vivo skin cell matrices) and associated methods of fabrication and use are provided. A holder including a microfluidic chamber includes multiple sidewall ports disposed at different levels, multiple inlet ports in communication with different sidewall ports, and a liquid-permeable membrane bounding a chamber bottom. Stratified layers of cell-containing material may be formed in the chamber by sequential steps of introducing cell-hydrogel precursor material through a sidewall port and crosslinking the same, then repeating the steps for each sidewall port, to form cell-containing material layers in contact with one another. A cover with distribution channel(s) is provided below the liquid-permeable membrane. A continuous upper extension of the chamber may be open from above to provide interface(s) for receiving analytes and / or for environmental interaction. A holder may removably receive multiple assay devices.

The present application relates to the use of recombinant collagen in the preparation of a preparation for increasing the expression of skin anti-wrinkle related genes, wherein the amino acid sequence of the recombinant collagen comprises the sequence shown in SEQ ID NO. 1. The present application detects the expression of a series of skin anti-wrinkle related genes by transcriptomics, and proves that the recombinant collagen provided by the present application can promote the expression of skin cell anti-wrinkle genes, and can be used for the development and application of various cosmetic products, and endows the cosmetic products with the efficacy and effect of promoting skin anti-wrinkle ability. The above-mentioned genes can also be used as a reference for evaluating the skin anti-wrinkle ability of other raw materials or cosmetics in the future.

The invention provides a marker for diagnosing and treating radiodermatitis and application of the marker. The relation between SENP5 and radiodermatitis is researched for the first time, it is found that SENP5 is in positive correlation with occurrence and development of radiodermatitis, an SENP5 overexpression group shows better radiation resistance, skin cells are protected, and damage caused by radiation is relieved. The SENP5 is mainly used for promoting DNA damage repair by mediating an HR repair pathway. Besides, the SENP5 knocked-down cell line is successfully constructed by designing shRNA for specifically knocking down the SENP5 expression level, and it is proved that after SENP5 knocked-down skin cells are irradiated by 60Co rays, the cell survival level is lowered, the homologous recombination repair pathway is obviously inhibited, the NF-kappa B pathway is obviously activated, and the inflammation level is improved. Therefore, SENP5 is represented as an anti-radiation gene in skin cells, and SENP5 can be used as a biomarker of radiodermatitis for early diagnosis of radiodermatitis and targeted preparation of drugs for radiodermatitis.

The invention discloses a preparation method of a mouse skin organoid, which comprises the following steps: separating dermis and epidermis of an E18.5 mouse to obtain dermis cells, mixing the dermis cells with back skin cells of an E14.5 embryo mouse, mixing by using Matrigel to form cell spheres, and gradually forming the skin organoid in in-vitro culture; the organoid is mainly applied to batch production of organoid containing hair follicles. The invention relates to the technical field of stem cells and regenerative medicine. According to the preparation method of the mouse skin organoid, a cell source is improved in the experiment process, the mouse skin organoid containing hair follicles can still be formed after cells in the early development stage are added, and related developmental biology research is carried out. And the source of another dermal cell is not limited to late pregnancy, and also can be a newborn mouse, so that the source of seed cells is expanded, and the cost is reduced.

The invention relates to the technical field of biological medicine, and discloses an active peptide energy liquid for skin repair as well as preparation and application thereof, and the active peptide energy liquid comprises the following raw materials in content range: 96-99% of water; the content of the trehalose is 0.5%-1.5%; the content of the oligopeptide-1 is 0.05%-0.15%; the content of the oligopeptide-3 is 0.01%-0.1%; the content of the heptapeptide-6 is 0.01%-0.1%; the content of sodium dihydrogen phosphate is 0.01%-0.1%; the content of disodium hydrogen phosphate is 0.1%-0.3%, three active peptide components including oligopeptide-1, oligopeptide-3 and heptapeptide-6 are introduced, and the characteristics of low molecular weight and high biological activity of active peptides are utilized, so that the active peptides can penetrate into skin cells and promote collagen synthesis, cell regeneration and skin barrier repair, and therefore, the anti-wrinkle, whitening and repairing capacities of the skin are improved, and the anti-wrinkle, whitening and repairing effects of the skin are improved. The technical means of strict temperature control, multi-stage stirring speed optimization and nitrogen protection are adopted in the preparation process to ensure that the active peptide is not aggregated or inactivated in the preparation process, and meanwhile, oxidation and microbial pollution are prevented.

A skin care composition includes a plurality of agents that include at least one retinol derivative and at least one bark extract, the plurality of agents providing reduced retinol associated inflammation as compared with a composition that includes the at least one retinol and lacks the at least one bark extract. The plurality of agents may include at least one retinol derivative comprising hydroxypinacolone retinoate and at least one bark extract which may include at least one bark extract comprising Eperua falcata bark extract. The plurality of agents may also include at least one hydroxy acid, for example, comprising lactic acid. The composition confers one or more of increased skin cell proliferation, improved cellular migration, improved cellular differentiation, or a combination thereof. Application the plurality of agents confers to skin cells and tissues an anti-inflammatory retinol genetic signature as demonstrated by via bulk-RNA sequencing of human keratinocytes and restores epidermal disruption and reduce IL-1A and IL-23 inflammatory cytokines in human ex-vivo skin models of chronic inflammation.

The present application relates to a kind of long stem ilex ginseng new use in skin care product and active composition and skin care product, product is emulsion, cream, water agent, spray, gel and other skin care products, the components of emulsion include: active composition, humectant, emollient, emulsifier, thickening agent and deionized water, the mass percentage content of active composition in skin care product is 0.005%-0.1%, active composition includes long stem ilex ginseng and wujin ginseng, the mass ratio of long stem ilex ginseng and wujin ginseng is 5:1-1:1, long stem ilex ginseng has at least one of the following effects: (1) UVB light damage pre-protection to skin;(2) after UVB light damage repair to skin;(3) up-regulate the trichohyalin gene expression in skin cell.The long stem ilex ginseng of the present application has the effects of UVB light damage pre-protection to skin, after UVB light damage repair to skin and up-regulating the trichohyalin gene expression in skin cell to repair skin barrier.

The invention relates to a saccharomyces cerevisiae cloned strain and application thereof. Specifically, the invention provides a saccharomyces cerevisiae clone strain A154 clone, a culture, a lysate or an extract and a preparation thereof, a yeast active substance derivative prepared from the saccharomyces cerevisiae clone strain A154 clone, and application of the saccharomyces cerevisiae clone strain A154 clone in preparation of a product for improving the irradiation resistance of skin cells.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a washing and caring two-in-one product containing a lactic acid bacteria fermentation extract and a preparation method of the washing and caring two-in-one product. Comprising the following raw materials in parts by weight: a thickening agent A, a skin conditioner, an anionic surfactant, a mild surfactant, cocamidomethyl, a thickening agent B, a chelating agent, a preservative, a moisturizing aid, a pH adjusting aid, an active matter and a proper amount of water. By adding the lactobacillus extract, the sodium thiosulfate and efficient and safe chemical components for compounding, the mild performance of the child shampoo shower gel is guaranteed, the powerful dechlorination function of the child shampoo shower gel is achieved, and the child shampoo shower gel has the skin conditioning function, is smaller in irritation, has the moisturizing and repairing effects and has a good application prospect. The lactobacillus fermentation extract has good moisturizing performance, can form a layer of moisturizing film on the skin surface, prevents moisture loss, keeps the skin moist and smooth, and also can promote metabolism of skin cells and enhance the moisturizing capacity of the skin.

This invention discloses an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in the indicated mass percentages: 22%~28% helichrysum essential oil, 22%~28% sweet basil essential oil, 22%~28% eucalyptol rosemary essential oil, and 22%~28% true lavender essential oil. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientifically formulated compound. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products.

The invention relates to the field of cosmetics. The invention relates more particularly to a method for the cosmetic care of sensitive skin, characterized in that it comprises the application to said sensitive skin of a composition comprising flocculosine as active agent, to obtain a soothing effect by stimulating the production of beta-endorphin and / or the expression of the CB2 receptor by the cells of said sensitive skin, said composition being optionally incorporated into a cosmetically acceptable excipient. The invention also relates to the non-therapeutic use of flocculosine to stimulate the production of beta-endorphin and / or the expression of the CB2 receptor by skin cells. Figure to be published with the abstract: Figure 2

The present invention discloses a bio-ink for DLP printing of bionic skin and its preparation and application, relating to the fields of biomedical materials and bioprinting. The bio-ink is composed of two skin ECM components that are chemically modified and proportioned. The modified photocurable collagen CoLMA and photocurable chondroitin sulfate CSMA both have the characteristics of rapid photocuring gel formation and good biocompatibility, which can meet the growth requirements of skin cells. Compared with single-component bio-ink, the composite CSMA-CoLMA bio-ink has better skin cell proliferation and adhesion promotion, and the gel storage modulus and elastic modulus values are close to those of natural skin. In addition, the two-component bio-ink is suitable for a variety of DLP printing equipment and printing methods on the market. The obtained cell-laden gel matrix can be adhered to the four sides of the Transwell chamber, meeting the construction requirements of bionic skin for cosmetic testing, and has clinical and commercial application value.

This invention relates to a skin cell dispersion for autologous treatment of skin wounds. Specifically, this invention provides a skin cell dispersion containing skin cells, epidermal growth factor, and transforming growth factor-β1. The skin cell dispersion of this invention exhibits excellent therapeutic effects on skin wounds.

The invention belongs to the technical field of polypeptides, and particularly relates to a novel synthetic polypeptide and a synthetic method and application thereof. The skin as the largest organ of the human body can visually reflect the aging level of the whole body. Skin senescence can be divided into endogenous senescence and exogenous senescence, the main manifestation of the cellular level is the senescence of fibroblasts in a corium layer, research shows that reactive oxygen species (ROS) is an important factor for promoting cell senescence, and the cell anti-senescence effect can be achieved by reducing the generation of ROS in cells. Melanin is a heterogeneous polyphenol-like polymer which is synthesized in melanin bodies and has a complex structure, the amount and distribution of the melanin not only determine the depth of the skin color, but also cause hyperpigmentation diseases due to metabolic disorder. In recent years, research finds that the polypeptide has excellent performance in skin care due to good biocompatibility and relatively strong biological activity. The polypeptide can rapidly permeate the skin through a specific receptor and activate the regeneration and repair functions of skin cells, so that the whitening and anti-aging effects are achieved. In addition, the action mechanism of the polypeptide is relatively mild, and the polypeptide is suitable for various skin types, especially sensitive skin. At present, although multiple polypeptide products exist in the market, most of the polypeptide products integrate a single function, and comprehensive products with double effects of whitening and anti-aging are lacked. Therefore, a polypeptide product with whitening and anti-aging effects is developed, the requirements of consumers can be better met, and the polypeptide product has important market value and application prospect.

This invention discloses the application of homoserine as an aging regulator in the preparation of anti-aging skin compositions. As a multidimensional regulator of skin aging, homoserine integrates skin cell lifespan regulation, telomere protection, and oxidative protection, providing a multi-target synergistic mechanism that can comprehensively and effectively delay skin aging, reduce wrinkles and sagging, and enhance vitality. The application of homoserine in cosmetics or skin care products meets consumers' demand for precise anti-aging skincare.