8 results about "Eucheuma" patented technology

The invention discloses an establishment method of a hippuris vulgaris callus regeneration system, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) selecting healthy stem segments of hippuris hispida with axillary buds, cleaning the healthy stem segments, and sequentially carrying out surface sterilization on the healthy stem segments by adopting 75% ethanol and a 0.1% mercuric chloride solution; (2) inoculating the sterilized explant into a callus induction culture medium, and inducing to generate a callus; (3) transferring the callus to a proliferation culture medium with the same formula for amplification; (4) transferring the proliferated calluses into an adventitious bud differentiation culture medium to induce adventitious buds; (5) separating the adventitious buds and inoculating the adventitious buds into a rooting and seedling strengthening culture medium to promote rooting and seedling strengthening; and (6) hardening the rooted seedlings and then transplanting the rooted seedlings. The method disclosed by the invention has the advantages of simple culture process, high proliferation efficiency, short period and the like, is suitable for large-scale production of aseptic seedlings, and can provide an effective technical platform for researches such as aquatic ecological restoration, medicine feed raw material supply, subsequent genetic transformation, germplasm preservation and the like.

The application provides a culture method for immobilized seedlings of sea tangle family seaweed and application thereof, and belongs to the technical field of seaweed propagation. The culture method comprises the following steps: fixing sea tangle family seaweed pure seed algae strains on natural coral stones or circular porous ceramic sheets, and placing the coral stones or the circular porous ceramic sheets in seawater for culture until the seed algae sprout secondary holdfasts; cutting the algae bodies sprouting the secondary holdfasts, reserving 0.1-1.0 cm algal body parts connected with the secondary holdfasts on the matrix, and continuing to culture the reserved parts together with the matrix in seawater until the reserved parts develop algal tips and grow into complete plants. The application induces the algae bodies to produce secondary holdfasts by using environment-friendly natural / artificial matrix, and then induces the secondary holdfasts to regenerate, so that a large number of immobilized sea tangle family seaweed seedlings are efficiently obtained, the problems of easy falling off of the algae bodies, low planting efficiency and pollution of the ecological environment in the existing sea tangle family seaweed releasing technology are solved, and the wind and wave resistance of the seedlings in open sea areas is improved.

The invention provides a culture method and application of eucheuma family seaweed immobilized offspring seeds, and belongs to the technical field of seaweed breeding. The culture method provided by the invention comprises the following steps: fixing eucheuma family seaweed purified seed algae strains on natural corallite or a circular porous ceramic chip, and culturing in seawater until the seed algae grow out of secondary fixators; cutting the frond from which the secondary fixation device is separated, reserving a 0.1-1.0 cm frond part connected with the secondary fixation device on the substrate, and continuously culturing the reserved part and the substrate in seawater until the reserved part develops a frond tip again and grows into a complete plant. According to the method, the algae are induced to generate the secondary fixation device through the environment-friendly natural / artificial substrate, then the secondary fixation device is induced to regenerate, a large number of eucheuma family algae seedlings which grow in an immobilized mode are efficiently obtained, and the problems that in an existing eucheuma family algae putting technology, the algae are prone to falling off, the planting efficiency is low, and the ecological environment is polluted are solved; meanwhile, the wind and wave resistance of the fries in open sea areas is improved.

The present application relates to the field of ecological restoration, and more particularly to a method for restoring the ecology of a coastal wetland, which comprises the following steps: removing invasive species from the area to be restored; laying an ecological restoration substrate; planting the sea grass in a cluster-strip composite pattern; introducing biological communities in batches every two months; setting up a tidal response system and an intelligent monitoring platform to monitor the condition of the area to be restored; and the ecological restoration substrate comprises 40% oyster shells, 40% coral sand and 20% bentonite. The method for restoring the ecology of a coastal wetland provided by the present application forms an "algae-grass barrier" by planting sea grass and eucheuma alternately, effectively improves the water transparency and promotes photosynthesis; the disturbance behavior of benthic animals such as sea cucumbers enhances the oxygen penetration depth and oxidation-reduction potential (Eh) of the sediments, and improves the bottom environment; and the gradient buoyancy planting device realizes tidal self-adaptation, ensuring the stable growth of the sea grass under different water level conditions.

The present utility model relates to a mechanized tropical seaweed harvester designed for branching-type seaweeds such as "Kappaphycus" and "Eucheuma". The harvester comprises a main frame, a gasoline engine, a pulley system, a horizontal shaft, a removable spool, adjustable support tubes, and a guide rack with a removable cutting plate equipped with a specialized designed cutting hole, all mounted on a floating platform supported by two small boats. The gasoline engine transmits rotational power through the pulley system to drive the spool, which retrieves the seaweed planting rope through the guide rack where the seaweed is separated and collected. The cleaned rope is simultaneously rewound for reuse in the next cultivation cycle. The harvester operates at an approximate speed of meters per minute, providing an efficient, safe, and continuous seaweed harvesting process, thereby reducing labor and time compared with traditional manual methods.

The invention belongs to the technical field of life cycle evaluation methods, and relates to a seaweed cultivation carbon footprint accounting method based on life cycle evaluation, and the life cycle evaluation method is used for evaluating various environmental loads of a seaweed cultivation system, namely kelp, undaria pinnatifida, laver, eucheuma and gracilaria. First, a life cycle boundary of a seaweed culture system is determined. Secondly, data such as material input, energy consumption and waste discharge of the system are collected, and life cycle list analysis is carried out; then, modeling is carried out on the breeding system by utilizing GaBi software, and the environmental influence of the breeding system is quantified by adopting a ReCiPe 2016 v1.1 Midpoint (H) method; and finally, comprehensively analyzing the result, identifying key links influencing carbon emission, and providing corresponding optimization and improvement measures. The invention provides a scientific basis for low-carbon development of global seaweed breeding industry.

The invention provides an enzymatic hydrolysate and a preparation method of a trochus pyramis Born digestive system enzyme extracting solution in the enzymatic hydrolysate, and also discloses a method for preparing tropical macroalgae eucheuma or kappaphycus protoplast by adopting the enzymatic hydrolysate and an application of the protoplast. The preparation and collection of the tropical macroalgae eucheuma / kappaphycus protoplast with high carrageenan content are realized for the first time by screening an enzyme extracting solution with a specific source and optimizing an enzymolysis system and process parameters, an obtained cell sample is free from common salt and protein residues, the complete cell collection amount is greater than or equal to 106 cells per 100 mL, the cell state is good, and the method is suitable for large-scale industrial production of the tropical macroalgae eucheuma / kappaphycus protoplast with high carrageenan content. The sample requirement of third-generation genome HIC sequencing can be met, and a key experimental material is provided for the basic research of genetic breeding and the like of the algae.

The present application aims to provide a eucheuma extract, a preparation method and application thereof, and the preparation method comprises the following steps: cutting eucheuma whole grass, using distilled water and 75% ethanol as extraction solvents respectively, hot reflux extraction twice, combining the filtrates, concentrating under reduced pressure, and evaporating the solvent to dryness to prepare a dry paste, and obtaining eucheuma water extract and eucheuma alcohol extract respectively. The eucheuma water extract and alcohol extract provided by the present application have significant inhibitory effects on staphylococcus aureus, escherichia coli, staphylococcus epidermidis, methicillin-resistant staphylococcus epidermidis, sarcina, bacillus, pseudomonas aeruginosa, enterococcus faecium, micrococcus luteus, acinetobacter baumannii, streptococcus pneumoniae and klebsiella pneumoniae, and have great development potential in the future research on bacteriostatic drugs, bacteriostatic cleaning products and other bacteriostatic products.