The application discloses a separation and purification preparation method of high-purity
gleditsia saponin monomer, and relates to the technical field of
phytochemistry. The method comprises the following steps: obtaining
gleditsia sinensis
raw material powder; adding the
gleditsia sinensis
raw material powder into an
ethanol solution, adjusting pH, performing ultrasonic extraction, collecting supernatant after
centrifugation, repeatedly extracting residues, combining the extraction solutions, and obtaining
gleditsia sinensis saponin crude extract; adding a flocculating agent into the crude extract, stirring,
sedimentation, suction
filtration, filtering through an
ultrafiltration membrane, and collecting permeate; loading the permeate into a macroporous adsorption resin column,
gradient elution, collecting target eluent, vacuum concentration, and obtaining
gleditsia sinensis saponin crude product;
gradient elution of the
gleditsia sinensis saponin crude product through a
silica gel column, collecting components containing gleditsioside A, high-performance liquid
chromatography refining, and obtaining gleditsioside A product; dissolving the gleditsioside A product in a mixed
solvent, dissolving, cooling, standing
crystallization, suction
filtration, washing, and obtaining a
crystal;
vacuum drying the
crystal, and obtaining high-purity gleditsia sinensis saponin
monomer gleditsioside A.