135 results about "Ternary complex" patented technology

The invention relates to the technical field of electronic elements, in particular to a ternary compound for a memristor array and a preparation method of the ternary compound. The ternary complex comprises A, B and C, A is a guest molecule of a pyridine derivative with an amino terminal, B is a bridging molecule, and C is a host molecule with a cucurbituril structure. A, B and C are self-assembled in an aqueous solution through Van der Waals force and hydrophobic interaction among molecules to form a ternary complex; the host molecule C has a barrel-shaped cavity structure, and the guest molecule A and the bridging molecule B are included in the barrel-shaped cavity structure. The ternary complex forms a supramolecular chain junction at the end part of the graphene electrode, so that the graphene electrode with different gaps can be adapted, and the molecular connection success rate of the array is greatly improved. The preparation method of the ternary complex is simple, convenient, easy to operate and suitable for large-scale production.

The invention discloses a cationic polymer-based proteolysis targeting chimera as well as preparation and application thereof, and belongs to the fields of targeting protein degradation and nanotechnology. According to the chimera, a cationic polymer is used as a connecting arm to connect a target protein ligand and an E3 ubiquitin ligase ligand to form a ternary complex, so that efficient degradation of target protein is realized. By adjusting the ligand proportion, chimeras with different degradation efficiencies can be synthesized, and accurate regulation and control are realized. The pH-responsive nano-drug obtained by hydrolyzing the targeting chimera by using the targeting protein can respond to an acidic tumor microenvironment, specifically release the chimera, and improve the targeting property and the treatment effect of the drug. Compared with the traditional PROTACs, the compound has higher bioavailability and tumor permeation effect; the preparation method of the nano-drug is simple and easy to implement, provides possibility for preparation of high-performance nano-drugs, shows potential in preparation of anti-tumor cell drugs, provides new thoughts and methods for tumor treatment, and has important clinical application value.

Disclosed herein are methods and compositions for preventing or reducing adverse events associated with administration of a RAS(ON) inhibitors. Thus, disclosed are compositions and methods for treating or preventing RAS(ON) inhibitor therapy-associated rash or mucositis. The methods involve administering a compound that binds to cyclophilin A (CypA) to compete with a RAS(ON) inhibitor for CypA binding, thereby reducing tri-complex formation in non-tumor tissues. These approaches may improve the tolerability of RAS(ON) inhibitor therapies without compromising efficacy.

The invention discloses a composition for treating diseases through protein degradation in a novel degradation way, and relates to the technical field of medicines. Comprising a mandolin-based androgen receptor degradation chimeric peptide (MbTACs), the mandolin-based androgen receptor degradation chimeric peptide (MbTACs) contains a targeting module YLCSSNNRERDKFRR combined with AR and a module combined with midnolin, a KLVFF sequence and a cell penetrating peptide PKKKPKV are added to the C end of the peptide, and a polypeptide molecule MbAD is formed. According to the invention, a midnolin-based MbTACs platform is developed, and the midnolin-based MbTACs platform is used for degrading specific proteins. The bifunctional molecules form a midnolin-MbTACs-target protein ternary complex between the target protein and the midnolin, and then the protein is degraded through a midnolin-proteasome way. The MbTACs can effectively degrade c-Myc and androgen antibodies in vitro and in vivo, and play an anti-tumor role. The MbTACs molecule represents a first midnolin-based target chimera, and the MbTACs molecule represents a first midnolin-based target chimera.

Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

The present invention provides a binary or ternary complex. The complex can improve the stability of a drug, and in particular, inhibit the transesterification reaction within drug molecules. The complex can also improve the efficacy formulation performance of the drug, improving the safety and effectiveness of the drug.

The invention discloses a digital single-molecule enzyme multi-activity characteristic cross-scale heterogeneity dynamic evaluation method, which comprises the following steps: diluting a pre-combined enzyme-template-primer ternary complex to a single-molecule level so as to load the enzyme-template-primer ternary complex into a reaction chamber in a micropore array chip for rolling circle amplification, a molecular beacon is combined with an amplification product to release a fluorescence signal; monitoring and acquiring the fluorescence signal in real time to generate a time-resolved fluorescence intensity curve; extracting a slope parameter of the fluorescence intensity curve so as to obtain a catalytic synthesis rate through conversion calculation for evaluating enzyme activity; the distribution of catalytic synthesis rate values is fitted using an n-fold Gaussian mixture model to obtain a standard deviation for assessing population homogeneity and a coefficient of variation for assessing stability. According to the invention, cross-scale analysis of single-molecule resolution, multi-parameter dynamic association and environmental interference processing can be realized at the same time, and resolution limitation of a traditional analysis technology is broken through by integrating single-molecule fluorescence tracking and population statistics.

The invention belongs to the technical field of gene engineering, and provides a gene OsMYB1 and application thereof in negative regulation of anthocyanin synthesis. The cDNA (complementary deoxyribonucleic acid) sequence of the gene OsMYB1 is as shown in SEQ ID NO. 1. A systematic molecular biology experiment proves that the OsMYB1 inhibits the expression of the OsDFR by combining with a promoter region of an anthocyanin synthesis key structural gene OsDFR, so that the synthesis of anthocyanin is inhibited. Meanwhile, the OsMYB1 can form an MBW ternary complex with the OsB2 and the OsPAC1 to competitively inhibit the synthesis of the anthocyanin, which indicates that the transcription factor OsMYB1 is a negative regulation factor for the synthesis of the rice anthocyanin, the content of the rice anthocyanin can be increased by knocking out the transcription factor OsMYB1, and a certain theoretical basis is provided for cultivating high-quality purple rice with high anthocyanin content.

The invention provides an MWCNTs-Au electrochemical biosensor based on RPA-CRISPR as well as a preparation method and application of the MWCNTs-Au electrochemical biosensor, and belongs to the technical field of preparation of electrochemical biosensors and detection of pathogenic bacteria of dairy products. The electrochemical biosensor disclosed by the invention comprises a multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode and a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-crRNA-Target DNA (Deoxyribose Nucleic Acid) ternary complex, the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe modified silk-screen printing carbon electrode, and the CRISPR-crRNA-Target DNA ternary complex is assembled on the surface of the multi-walled carbon nanotube-nanogold-hairpin nucleotide probe The electrochemical biosensor shows wide linear response in a range of 1.04 * 10 < 1 > to 1.04 * 10 < 8 > CFU / mL, and the detection limit is as low as 3 CFU / mL; and the method has good practicability in detection of staphylococcus aureus.

The invention discloses a kit, application and method for rapidly detecting mycoplasma synoviae based on an ERA-CRISPR / Cas12a one-step method, according to the visual detection kit, on the basis of a new nucleic acid detection target scpA of the mycoplasma synoviae, a sample to be detected is split by a splitting buffer to release genome DNA, then ERA-CRISPR / Cas12a one-pot reaction is carried out in a constant-temperature water bath kettle or a metal bath, and the sample to be detected is subjected to one-pot reaction, so that the ERA-CRISPR / Cas12a one-pot reaction is carried out to rapidly detect the mycoplasma synoviae through the ERA-CRISPR / Cas12a one-pot reaction. Under the mediation of a crRNA guide sequence, after Lb Cas12a enzyme is combined with crRNA and target DNA to form a ternary complex, the trans-shear activity of cas12a is activated, an ssDNA fluorescent probe in a reaction system can be arbitrarily cut to release a fluorescent group on the probe, and an ultraviolet lamp is used for detecting a fluorescent signal or a colloidal gold test strip is used for detecting whether FAM and Biotin groups are cut off for result judgment. According to the ERA-CRISPR / cas12a one-pot method technology for rapidly detecting the mycoplasma synoviae, the DNA target of the mycoplasma synoviae is recognized and cut through ERA amplification and CRISPR / cas12a specificity, the specificity is high, the detection sensitivity is as low as 3.81 pg / mu L, and the ERA-CRISPR / cas12a one-pot method technology can be used for rapidly detecting the mycoplasma synoviae in clinical samples on site.

The invention relates to the technical field of medicines, and discloses a stress particle targeted degradation agent as well as a preparation method and application thereof. The compound provided by the invention comprises a ternary complex composed of a stress particle protein, a linking group and an E3 ubiquitin ligase ligand or a ternary complex composed of a stress particle protein, a linking group and a ribonuclease L ligand, the compounds connected with different ligands have good targeting and degradation effects on the stress particles, can be used as a stress particle targeting degradation agent to be applied to preparation of anti-tumor drugs and anti-neurodegenerative disease drugs, and have good application prospects in treatment of stress particle induced tumor drug resistance and neurodegenerative diseases.

The invention discloses a preparation method of bread containing a ternary covalent compound. The preparation method comprises the following steps: preparing the following raw materials: 300 g of flour, 90-120 g of water, 4-6 g of yeast powder and 30-50 g of granulated sugar; the bread is prepared from, by weight, 1-3 g of salt, 1-2 eggs, 4-6 g of bread improver and 25-35 g of butter. 3 g to 6 g of a ternary covalent complex; the bread raw materials are mixed and kneaded into dough for primary fermentation, the dough is divided and put into a mold for secondary fermentation after primary fermentation, baking is conducted after secondary fermentation is completed, and finished bread is obtained after baking is completed. The protein-polysaccharide-polyphenol ternary complex is added into the bread, so that the oxidation resistance of the bread can be effectively improved, the digestion degree of procyanidine in the stomach is reduced, the absorption effect of intestinal tracts is improved, the bioavailability is improved, and meanwhile, the stability of the bread is also improved. The polyphenol is stable in the stomach, can be massively released in the intestinal tract after being digested in the intestinal tract, is favorable for maintaining the balance of beneficial bacteria and harmful bacteria in the intestinal tract, and promotes the health of the intestinal tract.

This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.

The present invention provides binary or ternary complexes capable of improving the stability of a drug, particularly inhibiting transesterification within a drug molecule. The compound can also improve the pharmacodynamic preparation performance of the medicine and improve the safety and effectiveness of the medicine.

The invention relates to the technical field of compositions of high-molecular compounds, and particularly discloses a preparation method of a sodium caseinate-epigallocatechin gallate-iron ion ternary complex as well as a product and application thereof. The preparation method comprises the following steps: S1, adding EGCG (epigallocatechin gallate) into an SC solution, and stirring in a shading environment to obtain a binary compound; s2, adding a FeCl3. 6H2O solution into the binary compound, and stirring in a shading environment to obtain a ternary compound; wherein in the ternary complex, the mass ratio of SC to EGCG to Fe < 3 + > is (10-14): 1: (2-4). According to the preparation method disclosed by the invention, the stable ternary complex can be prepared, the ternary complex utilizes the metal phenolic network to modify the sodium caseinate, and a high internal phase emulsion formed after the sodium caseinate is loaded with the beta-carotene can show excellent stability and relatively high encapsulation efficiency, so that the bioavailability of the beta-carotene can be remarkably improved.

The invention provides a protein-polysaccharide-polyphenol ternary complex used as a Pickering emulsifier and a preparation method of the protein-polysaccharide-polyphenol ternary complex. Specifically, the ternary complex is a complex formed by whey protein nanofibers and a pectin-polyphenol covalent complex. The ternary complex provided by the invention has very excellent interface stabilizer / active agent performance, and can be used as an emulsifier / interface stabilizer / interface active agent of Pickering emulsion.

The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.

The invention discloses a PROTAC chimera for targeted degradation of an ROR [gamma] t receptor and application of the PROTAC chimera, the structure of the chimera is RW-L-Re, Re is a ligand capable of being combined with E3 ubiquitin ligase, L is a linking group covalently combined with at least one Re and at least one RW, and Rw is a target protein ROR [gamma] t binding ligand and is selected from one of the following structures. A series of PROTAC chimeras capable of degrading an ROR gamma t receptor in a targeted manner are designed and synthesized for the first time, a ternary complex is formed mainly by combining a target protein ligand and an E3 ubiquitin ligase ligand with POI and E3 ligase respectively, and then the POI is labeled with a ubiquitination tag and is further degraded by proteasome. Experimental results prove that the PROTAC chimera designed by the invention has excellent ROR [gamma] t receptor degradation activity, and can be used for preparing related drugs for treating autoimmune diseases or tumors.

The invention discloses a medicinal and edible active component-small molecule peptide-nano-selenium synergistic anti-aging composition screened based on a molecular docking technology, and belongs to the technical field of functional foods and biological medicines. The composition comprises an active ingredient with homology of medicine and food, a specific molecular polypeptide Ala-Val-His-Leu-Cys-Gly-Phe-Asp-Glu-Ser-Thr-Lys-Arg-Tyr-Pro and nano-selenium, wherein the active ingredient with homology of medicine and food is screened by a molecular docking technology and is combined with senescence-related targets Nrf2 and / or SIRT1 in a high affinity manner, and the active ingredient, the specific molecular polypeptide and the nano-selenium form an active ingredient-molecular polypeptide-nano-selenium ternary complex with homology of medicine and food. The preparation method comprises the following steps: screening the medicinal and edible active ingredients meeting conditions, preparing the molecular polypeptide, mixing the medicinal and edible active ingredients to form a compound, adding the nano-selenium, stirring and combining, and freeze-drying to obtain a finished product. The composition can be prepared into an oral preparation or an external preparation, has a multi-dimensional synergistic efficient anti-aging effect of an active ingredient targeted activation pathway, direct anti-oxidation of nano-selenium and enhanced delivery of a peptide carrier, and is suitable for preparation of anti-aging drugs or functional foods.

The present invention provides a quantitative measurement method using competitive immunochromatography, which has not been previously reported. The quantitative measurement method comprises: (1) creating a calibration curve based on the signal intensity of a test line according to the concentration of a standard material by using multiple strips, and calculating the concentration of a target analyte by using the calibration curve; (2) when the concentration of the target analyte increases to a significant level on the calibration curve of the standard material, a labeled signal of a detection ligand on a control line remains unchanged in a case where a detection ligand of a target analyte-detection ligand conjugate binds to and saturates a capture ligand on the control line, making it impossible to estimate the concentration beyond that level; however, by designing a binding site for the capture ligand on the control line to be the target analyte of the target analyte-detection ligand conjugate, a high concentration of the target analyte breaks down the triple conjugate of detection ligand-target analyte-capture ligand, leading to dissociation of the labeled detection ligand and thereby weakening the signal (hook effect); and (3) comparing the signal intensity patterns of the test line and the control line according to the calibration curve of the standard material with the signal intensity pattern of a sample target analyte, and determining how far the target analyte is from the saturation point of the detection ligand. This method is characterized in that, in cases where the target analyte is present in an excessively large amount and exceeds the maximum value of a measurement range (dynamic range) of the calibration curve, the calibration curve using multiple strips and the configurations (test line and control line) of an immunochromatographic device are designed differently to distinguish such cases.

The present invention relates to the technical field of in-vitro testing, and in particular to a method for using a sandwich method to test a target molecule in a sample. The present invention provides a method for testing a target molecule in a sample under test, and testing is mainly performed by mean of a sandwich method, greatly improving the accuracy of testing of small molecules in serum. According to the method in the present invention, a complex-specific antibody specifically binding to a target molecule-target molecule binding protein is utilized to form a target molecule-target molecule binding protein-antibody ternary complex for testing. When a blood / serum sample containing the target molecule-target molecule binding protein is tested, in the method in the present invention, firstly, a specific dissociation solution is utilized to treat the sample under test so as to dissociate the target molecule from the target molecule-target molecule binding protein complex in the sample under test, so that the accuracy of testing of the target molecule can be further improved.

The application discloses a PROTAC compound with RORgamma t receptor targeted degradation and purposes thereof, which is a compound with a structure shown in a general formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutical composition thereof. e is a ligand capable of combining with an E3 ubiquitin ligase; the L is a linking group covalently combining at least one R e and at least one R W ; the R w is a target protein RORgamma t binding ligand; the application is based on the target point of RORgamma t and the PROTAC technology, and a series of RORgamma t-PROTACs are designed and synthesized for the first time. The mechanism is that the target protein ligand and the E3 ubiquitin ligase ligand are combined with POI and E3 ligase respectively, so as to form a ternary complex of "POI-PROTAC-E3 ligase". Then, the POI is marked with a ubiquitination label, and is degraded by a proteasome. The advantages of RORgamma t-PROTACs mainly include targeting of "undruggable proteins", small dosage, low toxicity, and difficulty in drug resistance. W -L-R e (I)

The application discloses a novel multifunctional resistant starch rich in active small-molecule ternary complex, a one-step induction synchronous preparation process and application thereof. By introducing lipids with a certain structure, phenolic compounds without long alkyl chains and starch form inclusion complexes with V-shaped ordered structures. By adjusting the addition sequence of lipids and phenolic compounds in the preparation process and optimizing the process parameters, multifunctional resistant starch with high phenolic loading rate and strong antioxidant activity is prepared by one-step induction. The application breaks through the technical bottleneck that phenolic compounds without long alkyl chains are difficult to form V-shaped complexes with starch, significantly improves the complexing efficiency of starch and phenolic compounds without long alkyl chain structure, and improves the content of active small-molecule phenols in the complex. Compared with the prior art, the prepared complex has stronger DPPH free radical scavenging effect, shows better digestion resistance and antioxidant effect, and can be used in the fields of functional food, nutritional additives and the like for reducing postprandial blood glucose, antioxidant and the like.

This invention belongs to the field of food processing technology, specifically relating to a walnut protein isolate-xanthan gum-epigallocatechin gallate ternary complex, emulsion, its preparation method, and its application. The walnut protein isolate-xanthan gum-epigallocatechin gallate ternary complex in the composite emulsion provided by this invention exhibits reduced particle size, enhanced electrostatic repulsion, and the absence of uneven clumps and particles. The elasticity and viscosity of the composite emulsion are significantly enhanced, and the complex demonstrates comprehensive environmental stability over a wide range of pH, salt concentration, and temperature conditions.

The invention relates to the field of bioengineering, in particular to a construction method and application of an amylopectin ternary nano assembly with a high assembly index. The invention provides a construction method of a ternary nano assembly. The construction method comprises the following steps: S1, extracting amylopectin of bulk crops by adopting an ethanol sedimentation method; s2, extracting endogenous lipids of fruits of completely mature bulk crops by adopting a supercritical carbon dioxide extraction method; s3, extracting endogenous proteins of fruits of immature bulk crops by adopting an isoelectric precipitation method; s4, the amylopectin, the endogenous lipid and the endogenous protein are subjected to a self-assembly reaction, and the ternary nano assembly is obtained. The particle size of the ternary complex can reach nanometer, and the ternary complex is high in CI, high in SDS content, low in multi-stage digestion rate, stable in blood sugar release process and good in chewiness.

The present application relates to the technical field of oyster peptides, and specifically to a method for preparing oyster peptides and their use in improving male reproductive function. The present application utilizes oyster peptides as raw materials, grafts oleanolic acid saponins thereon, and composites them with chitosan to prepare an emulsion of a ternary complex. The emulsion can form stable microscopic particles and has stable rheological properties. Through simulated digestion experiments, it was found that the emulsion has a sustained-release effect on the digestion of oyster peptides, and can at least reduce the proportion of digestion in the oral cavity. In addition, the use of the emulsion and the oyster peptide to intervene in rats with nephrotic syndrome can effectively alleviate their nephrotic syndrome, and at the same time promote the male sexual function of rats, and has an application prospect of modifying and improving male reproductive function.

The invention provides a magnetic control type electrochemical biosensor of MWCNTs-MoS2 based on CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a as well as a preparation method and application of the magnetic control type electrochemical biosensor. The magnetic control type electrochemical biosensor comprises an aminated multi-walled carbon nanotube-molybdenum disulfide modified screen-printed carbon electrode, wherein a CRISPR-crRNA-Target DNA ternary complex and a ferroferric oxide-gold-single-stranded nucleotide probe are dropwise added on the surface of the screen-printed carbon electrode, and the probe is sheared after the reaction of the CRISPR-crRNA-Target DNA ternary complex and the ferroferric oxide-gold-single-stranded nucleotide probe; the magnetic control type electrochemical biosensor respectively shows wide linear response in the range of 4.6 * 10 < 1 >-4.6 * 10 < 7 > CFU / mL of staphylococcus aureus and 5.5 * 10 < 7 > CFU / mL of salmonella, the detection limit of the magnetic control type electrochemical biosensor to two pathogenic bacteria is as low as 2CFU / mL, and the magnetic control type electrochemical biosensor has good specificity to interfering bacteria, can be used for rapid detection of food safety and has good practicability.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

This invention relates to the field of pharmaceutical technology, and discloses a stress particle targeted degrader, its preparation method, and its applications. The compounds provided by this invention include ternary complexes composed of stress particle protein, a linker group, and an E3 ubiquitin ligand, or ternary complexes composed of stress particle protein, a linker group, and a ribonuclease L ligand. Compounds with different ligands exhibit good targeting and degradation effects on stress particles and can be used as stress particle targeted degraders in the preparation of antitumor drugs and anti-neurodegenerative disease drugs, showing promising application prospects in treating stress particle-induced tumor drug resistance and neurodegenerative diseases.

Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li+, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li+, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca2+. In addition to Li+, the fluid can contain polyethylenimine (PEI) with or without betaine.