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61 results about "Ternary complex" patented technology
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A ternary complex is a protein complex containing three different molecules that are bound together. In structural biology, ternary complex can also be used to describe a crystal containing a protein with two small molecules bound, for example cofactor and substrate; or a complex formed between two proteins and a single substrate. In Immunology, ternary complex can refer to the MHC–peptide–T-cell-receptor complex formed when T cells recognize epitopes of an antigen. Some other example can be taken like ternary complex while eukaryotic translation, in which ternary complex is composed of eIF-3 & eIF-2 + Ribosome 40s subunit+ tRNAi. A ternary complex can be a complex formed between two substrate molecules and an enzyme. This is seen in multi-substrate enzyme-catalyzed reactions where two substrates and two products can be formed. The ternary complex is an intermediate between the product formation in this type of enzyme-catalyzed reactions. An example for a ternary complex is seen in random-order mechanism or a compulsory-order mechanism of enzyme catalysis for multi substrates.
Therapeutic compositions and methods for managing treatment-related effects
PCT designated stageWO2025265060A1Organic active ingredientsDigestive systemCyclophilin GTolerability
Disclosed herein are methods and compositions for preventing or reducing adverse events associated with administration of a RAS(ON) inhibitors. Thus, disclosed are compositions and methods for treating or preventing RAS(ON) inhibitor therapy-associated rash or mucositis. The methods involve administering a compound that binds to cyclophilin A (CypA) to compete with a RAS(ON) inhibitor for CypA binding, thereby reducing tri-complex formation in non-tumor tissues. These approaches may improve the tolerability of RAS(ON) inhibitor therapies without compromising efficacy.
Owner:REVOLUTION MEDICINES INC
A cordycepin-loaded protein-glycosan ternary complex nanoparticle, a preparation method and application thereof
This invention discloses a protein-polysaccharide ternary nanoparticle loaded with cordycepin, its preparation method, and its applications. The invention employs a layer-by-layer self-assembly method to modify the surface of zein nanoparticles. Since zein has a positive surface charge, the first layer modification uses the anionic polysaccharide sodium alginate for electrostatic bonding, and the second layer modification uses the cationic polysaccharide chitosan, constructing positively charged ternary composite nanoparticles. These nanoparticles have small particle size, high encapsulation efficiency, high drug loading capacity, and a sustained-release effect, significantly reducing cytotoxicity and significantly improving the therapeutic effect on osteoarthritis. This invention develops a novel cordycepin formulation, overcoming the limitations of cordycepin's clinical use.
Owner:CHANGZHOU UNIV
Polypeptide synergistically enhanced cubilose collagen composite beverage with breast enlarging function and preparation method thereof
The invention relates to the technical field of functional drinks, in particular to a polypeptide synergistically strengthened cubilose collagen composite drink with a breast enlarging function and a preparation method of the polypeptide synergistically strengthened cubilose collagen composite drink. The drink contains fish collagen tripeptide, elastin peptide, cubilose peptide, pueraria mirifica isoflavone, red clover isoflavone and saffron crocus extract. A lipidosome delivery system is adopted to entrap polypeptide to form a nano-scale compound, nano-emulsion entraps phytoestrogen, and berry polyphenol and lipidosome-polypeptide form a stable ternary compound. Through the multi-way synergistic effect that phytoestrogen activates a mammary gland estrogen receptor, polypeptide promotes connective tissue collagen synthesis and cubilose peptide activates a growth factor signal, mammary gland development is remarkably promoted, the breast enlarging effect is achieved, the bioavailability reaches 85% or above, and the retention rate of active ingredients stored at normal temperature for 24 months is larger than 90%.
Owner:BEIJING SHANTU BIOTECHNOLOGY CO LTD
PROTAC chimera for targeted degradation of ROR [gamma] t receptor and application of PROTAC chimera
The invention discloses a PROTAC chimera for targeted degradation of an ROR [gamma] t receptor and application of the PROTAC chimera, the structure of the chimera is RW-L-Re, Re is a ligand capable of being combined with E3 ubiquitin ligase, L is a linking group covalently combined with at least one Re and at least one RW, and Rw is a target protein ROR [gamma] t binding ligand and is selected from one of the following structures. A series of PROTAC chimeras capable of degrading an ROR gamma t receptor in a targeted manner are designed and synthesized for the first time, a ternary complex is formed mainly by combining a target protein ligand and an E3 ubiquitin ligase ligand with POI and E3 ligase respectively, and then the POI is labeled with a ubiquitination tag and is further degraded by proteasome. Experimental results prove that the PROTAC chimera designed by the invention has excellent ROR [gamma] t receptor degradation activity, and can be used for preparing related drugs for treating autoimmune diseases or tumors.
![PROTAC chimera for targeted degradation of ROR [gamma] t receptor and application of PROTAC chimera](https://images-eureka.patsnap.com/patent_img/0099eb6b-59f3-496b-9b48-3b4bcd0820a5/CN121895286A.png "PROTAC chimera for targeted degradation of ROR [gamma] t receptor and application of PROTAC chimera")
Owner:ZHENGZHOU UNIV
A protac compound with rorγt receptor targeted degradation and uses thereof
The application discloses a PROTAC compound with RORgamma t receptor targeted degradation and purposes thereof, which is a compound with a structure shown in a general formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutical composition thereof. e is a ligand capable of combining with an E3 ubiquitin ligase; the L is a linking group covalently combining at least one R e and at least one R W ; the R w is a target protein RORgamma t binding ligand; the application is based on the target point of RORgamma t and the PROTAC technology, and a series of RORgamma t-PROTACs are designed and synthesized for the first time. The mechanism is that the target protein ligand and the E3 ubiquitin ligase ligand are combined with POI and E3 ligase respectively, so as to form a ternary complex of "POI-PROTAC-E3 ligase". Then, the POI is marked with a ubiquitination label, and is degraded by a proteasome. The advantages of RORgamma t-PROTACs mainly include targeting of "undruggable proteins", small dosage, low toxicity, and difficulty in drug resistance. W -L-R e (I)
Owner:ZHENGZHOU UNIV
A new type of multifunctional resistant starch rich in active small molecule ternary complex, one-step induction synchronous preparation process and application thereof
The application discloses a novel multifunctional resistant starch rich in active small-molecule ternary complex, a one-step induction synchronous preparation process and application thereof. By introducing lipids with a certain structure, phenolic compounds without long alkyl chains and starch form inclusion complexes with V-shaped ordered structures. By adjusting the addition sequence of lipids and phenolic compounds in the preparation process and optimizing the process parameters, multifunctional resistant starch with high phenolic loading rate and strong antioxidant activity is prepared by one-step induction. The application breaks through the technical bottleneck that phenolic compounds without long alkyl chains are difficult to form V-shaped complexes with starch, significantly improves the complexing efficiency of starch and phenolic compounds without long alkyl chain structure, and improves the content of active small-molecule phenols in the complex. Compared with the prior art, the prepared complex has stronger DPPH free radical scavenging effect, shows better digestion resistance and antioxidant effect, and can be used in the fields of functional food, nutritional additives and the like for reducing postprandial blood glucose, antioxidant and the like.
Owner:TIANJIN UNIV OF SCI & TECH
A walnut protein-xanthan gum-epigallocatechin gallate ternary complex, emulsion and preparation method and application thereof
This invention belongs to the field of food processing technology, specifically relating to a walnut protein isolate-xanthan gum-epigallocatechin gallate ternary complex, emulsion, its preparation method, and its application. The walnut protein isolate-xanthan gum-epigallocatechin gallate ternary complex in the composite emulsion provided by this invention exhibits reduced particle size, enhanced electrostatic repulsion, and the absence of uneven clumps and particles. The elasticity and viscosity of the composite emulsion are significantly enhanced, and the complex demonstrates comprehensive environmental stability over a wide range of pH, salt concentration, and temperature conditions.
Owner:AGRO PROD PROCESSING RES INST YAAS
A method for preparing a casein micelle-phenol-whey protein ternary complex
This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.
Owner:ANHUI AGRICULTURAL UNIVERSITY +1
Stress granule targeting degraders, methods of making and using the same
This invention relates to the field of pharmaceutical technology, and discloses a stress particle targeted degrader, its preparation method, and its applications. The compounds provided by this invention include ternary complexes composed of stress particle protein, a linker group, and an E3 ubiquitin ligand, or ternary complexes composed of stress particle protein, a linker group, and a ribonuclease L ligand. Compounds with different ligands exhibit good targeting and degradation effects on stress particles and can be used as stress particle targeted degraders in the preparation of antitumor drugs and anti-neurodegenerative disease drugs, showing promising application prospects in treating stress particle-induced tumor drug resistance and neurodegenerative diseases.
Owner:SUN YAT SEN UNIV
Multifunctional ternary complex based on cyclodextrin and preparation method thereof
The invention relates to the technical field of biological medicine, in particular to a multifunctional ternary complex based on cyclodextrin and a preparation method thereof. The invention provides a multifunctional curcumin / methotrexate ternary complex based on beta-cyclodextrin. The ternary complex can realize synergistic delivery and controlled-release release of natural polyphenol and synthetic drugs in a medication mode of'one dose with multiple effects'. Meanwhile, the problems that a traditional medicine is poor in water solubility and low in bioavailability, the treatment effect of a single medicine is limited, and an existing nano delivery system is complex in structure, tedious in preparation and the like are solved, and the technical requirements of modern medicine preparations are met.
Owner:XINYANG NORMAL UNIVERSITY
A stable ternary complex instant milk tea based on kappa-casein genotype and a preparation method thereof
This invention provides a ready-to-drink milk tea based on a ternary complex stabilized with κ-casein genotype and its preparation method, belonging to the field of dairy processing technology. It includes a ternary complex of casein micelles containing AA genotype κ-casein, epigallocatechin gallate, and fructose. The preparation of the ready-to-drink milk tea includes the following steps: S1: raw material screening and pretreatment; S2: preparation of the ternary complex; S3: blending and processing of the ready-to-drink milk tea. By screening AA genotype κ-casein raw milk and using a specific sequence of first compounding EGCG and then grafting fructose, a casein micelle-EGCG-fructose ternary complex with uniform structure and controllable particle size is constructed. Fructose is further grafted onto the surface of the complex through glycosylation, effectively masking the excessive binding sites of proteins and polyphenols, significantly inhibiting the formation of insoluble aggregates, thereby maintaining excellent colloidal stability of the product during its shelf life.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Rare earth organic acid salt complex with high far infrared emissivity as well as preparation method and application thereof
PendingCN121378296AEnergy modified materialsGroup 3/13 element organic compoundsOrganic acidTernary complex
The invention provides a rare earth organic acid salt complex with high far infrared emissivity and a preparation method and application thereof, the molecular general formula of the rare earth organic acid salt complex is RE (OA-SH) 3 (Phen), RE is a rare earth ion, OA-SH is a functionalized oleic acid ligand, Phen is phenanthroline, and the functionalized oleic acid ligand is 11-mercaptoundecane-9-olefine acid. According to the invention, the ternary complex with a clear structure is successfully constructed through a multi-step reaction. Compared with simple neodymium oleate, the ternary complex has the advantage that the far infrared emissivity can be increased from 0.92 to 0.97.
Owner:TIANJIN BAOGANG RES INST OF RARE EARTHS CO LTD +1
Cas protein pam analysis method based on local geometry and machine learning
The application discloses a Cas protein PAM analysis method based on local geometric structure and machine learning, and belongs to the fields of biotechnology and artificial intelligence. The method comprises the following steps: constructing an initial structure of a Cas protein-DNA-RNA ternary complex by using a structure prediction tool, and generating a mutant structure data set containing different PAM sequences by calculation and optimization; extracting and embedding the local structure of a PAM site to obtain a structure feature vector, performing dimension reduction and cluster analysis by using an unsupervised machine learning method, realizing natural classification of the PAM sequence, and identifying the cluster result in combination with P prior biological knowledge. The application creatively combines the three-dimensional local geometric structure of a protein and machine learning, can predict and classify the PAM preference of a Cas protein from a structure source, has the advantages of objectivity, comprehensiveness, and the ability to reveal hidden patterns, and can be verified by experimental data.
Owner:ZHEJIANG LAB
Ternary protein-based composite system and preparation method thereof
The present disclosure discloses a ternary protein-based composite system and the preparation method thereof, relating to the fields of functional foods and pharmaceuticals. Compared to a single soy protein isolate and the binary complexes thereof, the ternary composite systems SLE-7, SEL-7, SLE-9, SEL-9 exhibit significant changes in protein structure and stronger interactions. Studies shows that pH value and the addition sequence of raw materials significantly impact the particle size of the complexes and functional properties of the composite systems. At pH 7, SLE-7 shows the highest EAI and ESI among ternary complexes; at pH 9, SEL-9 shows the highest EAI and ESI among the ternary systems; SEL-9 possesses the smallest particle size and the most uniform system, with its composite emulsion demonstrating optimal emulsifying performance, making it suitable for application in functional foods and pharmaceuticals.
Owner:JILIN AGRICULTURAL UNIV
A soluble dietary fiber composition, a method for preparing the same, and applications thereof
The present application provides a soluble dietary fiber composition, its preparation method and application. After the soluble dietary fiber composition enters the colon, the ester bond in the alanine modified pectin is rapidly degraded, part of the chlorogenic acid is released, the secretion of anorectic hormone is stimulated, the appetite is reduced, and the heat intake is reduced; the remaining amide bond network is slowly degraded, realizing the slow and continuous release of chlorogenic acid. Lutein removes free radicals generated by fat decomposition, reducing the oxidative damage of L cells. Galactomannan and alanine modified pectin-glucan modified protein-chlorogenic acid ternary complex synergize to improve the molar ratio of butyric acid / propionic acid: propionic acid enhances satiety through intestinal gluconeogenesis, and butyric acid stimulates the secretion of GLP-1 / PYY, realizing endogenous appetite suppression. Galactomannan absorbs water to form a lubricating gel, softening the stool and increasing the stool volume; the ternary complex degrades to produce short-chain fatty acids, optimizing the intestinal microenvironment and enhancing gastrointestinal peristalsis, both of which synergistically improve constipation.
Owner:BEIJING GUAR SCI&TRADING
Digital single-molecule enzyme multi-activity characteristic cross-scale heterogeneity dynamic evaluation method
The application discloses a kind of digital single molecule enzyme multi-activity characteristic cross-scale heterogeneity dynamic evaluation methods, comprising: the enzyme-template-primer ternary complex of pre-combination completion is diluted to single molecule level to be loaded into the reaction chamber in microwell array chip to carry out rolling circle amplification, and utilize molecular beacon and the fluorescence signal released by amplification product combination;Real-time monitoring and obtaining the fluorescence signal to generate time-resolved fluorescence intensity curve;The slope parameter of the fluorescence intensity curve is extracted to obtain the catalytic synthesis rate by conversion calculation for evaluating enzyme activity;The catalytic synthesis rate value distribution is fitted using n times Gaussian mixture model to obtain the standard deviation for evaluating population uniformity and the coefficient of variation for evaluating stability.The application can realize single molecule resolution, multi-parameter dynamic correlation and environmental interference processing cross-scale analysis simultaneously, and break through the resolution limit of traditional analysis technology by integrating single molecule fluorescence tracking and population statistics.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Use of chlorogenic acid in the preparation of a drug for treating neuronal intranuclear inclusion disease
PendingCN122124030APromote dependent degradationStrong specificityOrganic active ingredientsNervous disorderDiseaseChlorogenic acid
This invention provides the application of chlorogenic acid in the preparation of drugs for treating intranuclear inclusion body disease (NIID), belonging to the field of pharmaceutical technology. This invention utilizes widely available sources with abundant safety data. Compared to novel chemical entities, chlorogenic acid, with its lower development risk, is used as a raw material. It has been found that chlorogenic acid can enhance the interaction between polyG protein and the E3 ubiquitin ligase TRIM21, forming a stable TRIM21-chlorogenic acid-polyG ternary complex. This promotes polyG ubiquitination and proteasome-dependent degradation, thereby reducing intracellular inclusion body burden and alleviating NIID from its pathological root cause. This provides a safe, effective, and highly specific new targeted therapeutic strategy for NIID.
Owner:XIANGYA HOSPITAL CENT SOUTH UNIV
Compounds, conjugates, and methods for preparing and using them
The present invention features compounds containing an aziridine moiety and methods for synthesizing the same. The compounds can be linked to a monovalent organic moiety and can be used to bind to a target (e.g., a target protein), e.g., by crosslinking to the target. In some embodiments, the monovalent organic moiety is capable of binding to a presenter protein. Also disclosed are complexes containing the compounds (e.g., presenter protein / compound complexes, compound / target protein complexes, or ternary complexes). Also disclosed are methods of forming the complexes and methods of modulating biological processes using the compounds and complexes.
Owner:REVOLUTION MEDICINES INC
A type 1 diabetes vaccine based on self-antigen polypeptide molecules
This invention discloses a type 1 diabetes vaccine based on autoantigen polypeptide molecules. The invention provides a series of immunogenic autoantigens as active ingredients in type 1 diabetes vaccines. This invention utilizes computer-simulated amino acid mutations of type 1 diabetes autoantigen sequences obtained from type 1 diabetes patients, supplemented by rational design based on the HLA-peptide-TCR ternary complex structure; targeted optimization enhances the binding affinity between the antigen and immune molecules, thereby achieving significant proliferation of type 1 diabetes-related CD4+ T lymphocytes. The autoantigens of this invention will serve as the basis for the development of type 1 diabetes vaccines in the form of artificially synthesized polypeptide molecules.
Owner:SHANGHAI INST FOR ADVANCED STUDY OF ZHEJIANG UNIV
Compound preparation suitable for traditional Chinese medicine constitution conditioning and preparation method thereof
The invention discloses a compound preparation suitable for traditional Chinese medicine constitution conditioning and a preparation method thereof. The compound preparation comprises a stepped ternary-quaternary-quinary compound preparation. The ternary complex is based on hydroxypropyl methyl cellulose, fumed silica and silicified microcrystalline cellulose, and digestive tract mucosa protection and body safety tolerance establishment are achieved; in the quaternary compound, resistant dextrin is added on the basis of the ternary compound, so that the digestion function and the defecation rhythm are stable; l-glutamine is further added to the quinary compound on the basis of a quaternary compound, so that the whole body state is harmonized, and nine constitutions in traditional Chinese medicine are balanced. The three components strictly follow a ternary-quaternary-quinary sequence for use, and a complete conditioning path from safe tolerance establishment to intestinal environment regulation to whole body consolidation is formed. The invention provides a preparation process which is high in forming rate, good in stability, resistant to moisture absorption, excellent in reconstituability and suitable for industrial large-scale production.
Owner:ZHEJIANG DEJU RENHE THINK TANK TECHNOLOGY CO LTD
Zein-osa starch-tween-20 ternary complex pickering particles, and preparation method and application thereof
The application belongs to the technical field of nanoparticle application, and particularly relates to a corn alcohol-soluble protein-OSA starch-tween-20 ternary composite Pickering particle and a preparation method and application thereof. The corn alcohol-soluble protein-OSA starch-tween-20 ternary composite Pickering particle and emulsion thereof are constructed under the condition of different concentrations of tween-20, the problems of instability of a corn alcohol-soluble protein-OSA starch composite system and easy stratification and flocculation in a storage process are solved, and the stability of the corn alcohol-soluble protein-OSA starch-tween-20 ternary composite Pickering particle and emulsion thereof is improved by adjusting the proportion of the content of tween-20 in the ternary composite system.
Owner:CHINA AGRI UNIV
Oligopeptides and their use in the preparation of anti-aging products
This invention relates to the field of cosmetics, and more particularly to oligopeptides and their application in the preparation of anti-aging products. This invention provides an oligopeptide with anti-aging activity, the amino acid sequence of which is shown in any of SEQ ID NO:1 to SEQ ID NO:3. This invention rationally designs and optimizes three α-Klotho-derived oligopeptides (RBA-1, RBA-1d, and RBA-1e) with a clear structural basis and optimized physicochemical properties through high-resolution crystal structure information of the FGF23-FGFR1c-αKlotho ternary complex, systematic truncation screening of the receptor binding arm (RBA), and conformation prediction, for use in skin anti-aging.
Owner:GUANGZHOU JIYAN COSMETICS TECH CO LTD +1
Method for rapid separation and detection of berberine and epiberberine based on trapping ion mobility mass spectrometry
This invention discloses a method for rapid separation and detection of berberine and epiberberine based on ion mobility mass spectrometry. The specific steps are as follows: berberine and epiberberine in the sample to be tested are mixed with cucurbituril [8] and magnesium ions to form a ternary complex; the obtained ternary complex is ionized using an electrospray ionization source; different complex ions are separated using nitrogen as the drift gas in the ion mobility mode; the separated ions are detected by tandem mass spectrometry; qualitative analysis of berberine and epiberberine is achieved based on the triple information of characteristic mass-to-charge ratio, mobility value and collision cross section; and quantitative analysis of berberine and epiberberine is achieved by external standard method or internal standard method. This invention uses cucurbituril [8] as a chiral auxiliary reagent and magnesium ions as a metal complexing agent, and uses ion mobility-mass spectrometry to distinguish berberine isomers, which can rapidly, with high resolution and high accuracy, separate and detect berberine and epiberberine.
Owner:HENAN NORMAL UNIV
Ternary composite crystal form conversion accelerant and method for preparing ultrapure titanium dioxide by utilizing ternary composite crystal form conversion accelerant
The invention discloses a ternary composite crystal form conversion accelerant and a method for preparing ultra-pure titanium dioxide through the ternary composite crystal form conversion accelerant, and relates to the field of material preparation, the ternary composite crystal form conversion accelerant comprises a substance A, a Zn (NO3) 2 aqueous solution and Nb2O5 powder, the substance A is Sb-doped SnO2 crystals, the molar ratio of Sb / (Sn + Sb) is 3%-8%, and the molar fraction of Sb < 5 + > in all Sb elements is larger than or equal to 80%; the oxygen vacancy concentration of the substance A is 5 * 10 < 18 > cm <-3 > to 2 * 10 < 19 > cm <-3 >; the surface hydroxyl density of the substance A is 2.5 mmol / g to 4.0 mmol / g, and the free hydroxyl accounts for more than or equal to 70%; the Nb2O5 powder is in a monoclinic phase, the crystallinity is 65%-80%, and the specific surface area is 50 m < 2 > / g-80 m < 2 > / g. The ternary composite crystal form conversion accelerator can reduce the calcination temperature and realize low-temperature calcination to prepare ultra-pure titanium dioxide.
Owner:HUBEI TIANCI ELECTRONICS MATERIALS CO LTD
Use of small molecule inhibitors of lactate dehydrogenase a in the preparation of antiviral medicaments
The present application relates to the technical field of new antiviral drug active ingredients, and particularly relates to application of a small-molecule compound inhibitor of lactate dehydrogenase A in preparation of an antiviral drug, wherein the inhibitor is Kinetin or L67, which can be directly combined with lactate dehydrogenase A (LDHA), on one hand, significantly reduces the expression level of LDHA, reduces the negative regulation of LDHA to the IFN-I channel from the source; on the other hand, can specifically destroy the LDHA / STAT2 / HDAC3 ternary complex induced by IFN-I, antagonize the transcriptional regulation function of LDHA, and release the inhibition of the antiviral effect of IFN-I. The present application can solve the core pain point that the response rate of IFN-alpha in the treatment of chronic hepatitis B (CHB), influenza virus or new coronavirus and other viral infection diseases is not high in clinic; through the combined scheme of Kinetin / L67+IFN-I, the response efficiency of clinical interferon treatment is improved.
Owner:ACADEMY OF MILITARY MEDICAL SCIENCES
Targeted protein degradation and recruitment
PCT designated stageWO2026017888A1Pharmaceutical non-active ingredientsAntineoplastic agentsHydrophilic polymersProtein target
The present invention pertains to the field of targeted protein degradation (TPD) and target protein recruitment (TPR) providing a versatile platform for TPD, TPR and other applications dependent on cell surface ternary complex formation. In particular, the present invention provides a compound comprising a macromolecular hydrophilic polymer scaffold which is conjugated with several copies of at least two different protein binding ligands. The polymeric scaffold of the invention enables the use of small molecule ligands for target proteins of interest. Furthermore, the invention also relates to a composition comprising said polymeric scaffolds and uses thereof, e.g. for inhibiting or removing malignant or unwanted proteins; or for use in the targeted recruitment of effector cells such as CAR-T cells.
Owner:UNIV GENT
Gene osmyb1 and its application in negative regulation of anthocyanin synthesis
The application belongs to the technical field of genetic engineering, and provides a gene OsMYB1 and application of the gene in negative regulation of anthocyanin synthesis; the cDNA sequence of the gene OsMYB1 is shown as SEQ ID NO. 1. The application proves by systematic molecular biology experiments that OsMYB1 can inhibit the expression of OsDFR by combining with the promoter region of the key structural gene OsDFR of anthocyanin synthesis, thereby inhibiting the synthesis of anthocyanin. Meanwhile, OsMYB1 can also form an MBW ternary complex with OsB2 and OsPAC1, competitively inhibits the synthesis of anthocyanin, and indicates that the transcription factor OsMYB1 is a negative regulation factor of anthocyanin synthesis in rice, and knocking out the same can increase the content of anthocyanin in rice, thereby providing certain theoretical basis for cultivating high-quality purple rice with high anthocyanin content.
Owner:XICHANG COLLEGE
An immunogenicity prediction system and method for MHC-antigen peptide-TCR ternary complex
This invention discloses an immunogenicity prediction system and method for the MHC-antigen peptide-TCR ternary complex. The system includes a data input and dataset construction module, a multimodal feature dual-branch extraction module, a multimodal contrastive learning and fusion module, and an activity prediction and output module. The data input and dataset construction module includes a raw data input terminal, a hard negative sample generation unit, and a homology isolation and segmentation unit. The multimodal feature dual-branch extraction module includes a one-dimensional sequence feature extraction branch and a three-dimensional topological graph feature extraction branch. The activity prediction and output module includes a fully connected classifier and a prediction output terminal. This invention solves the technical problems of high false positives, poor generalization ability, and lack of spatial feature extraction in existing technologies.
Owner:JIANGXI AKSUO LIFE HEALTH TECHNOLOGY CO LTD
Methods and compositions for capping nucleic acids
A method for identifying a nucleic acid template that include (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3′ end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.
Owner:PACIFIC BIOSCIENCES OF CALIFORNIA INC
Multi-mechanism molecular glue inducibility prediction method
The invention discloses a multi-mechanism molecular gel inducibility prediction method, relates to the technical field of computer-aided drug design, and discloses a multi-mechanism molecular gel inducibility prediction method which combines multi-modal features such as a one-dimensional sequence, a two-dimensional graph structure and a three-dimensional structure of a coding small molecule and two types of proteins to predict the inducibility of the multi-mechanism molecular gel. A three-element cross-entity feature interaction mechanism is introduced, unified prediction of the inducibility of the ternary complex under different E3, substrates and chemical space backgrounds is realized, prediction requirements under different E3, different substrates and diversified chemical space backgrounds can be met, the influence of the conformational collaboration of the ternary complex on the inducibility is comprehensively captured, and the method has the advantages of being high in adaptability and high in practicability. And the prediction efficiency and applicability of the molecular glue are improved.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA