Method for preparing fibroin nano-silver hydrosol

A silk protein and hydrosol technology, applied in the field of nanomaterials, can solve problems such as resource waste and environmental pollution, and achieve the effects of wide range of raw materials and simple and effective preparation methods.

Inactive Publication Date: 2013-02-20
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] my country is the largest producer of silk, and a large amount of waste silk is produced every year, and 20-30% of the weight of silk is seric

Method used

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  • Method for preparing fibroin nano-silver hydrosol
  • Method for preparing fibroin nano-silver hydrosol
  • Method for preparing fibroin nano-silver hydrosol

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Disperse 0.2g of cornstarch in 10ml of deionized water, stir at 80°C until gelatinized to obtain a cornstarch solution, add it to 10ml of silver nitrate solution with a concentration of 0.05mol / L, and mix evenly to obtain a starch-silver nitrate binary compound solution.

[0027] Mix papain and sericin solution at a mass ratio of 1:20, adjust the pH value and temperature to the optimum conditions for the activity of the corresponding protease, and stop the reaction after hydrolyzing for 40 minutes under stirring conditions. The pore filter membrane is filtered, and the filtrate is concentrated and dried to obtain sericin peptide powder with a molecular weight of 1-5 KDa.

[0028] Take 2.4g small molecule sericin peptide powder and dissolve it in 120ml deionized water to obtain a sericin peptide solution with a concentration of 2 wt %, add it to the above-mentioned starch-silver nitrate composite solution, and mix evenly to obtain sericin peptide - The ternary composite...

Embodiment 2

[0030] Disperse 0.15g of tapioca starch in 5ml of deionized water, stir at 90°C until gelatinized to obtain a tapioca starch solution, add it to 20ml of silver nitrate solution with a concentration of 0.2mol / L, mix well to obtain starch-silver nitrate binary compound solution.

[0031] Take 3g of small-molecule silk fibroin peptide powder with a molecular weight of 1-5KDa and dissolve it in 100ml of deionized water to obtain a silk fibroin peptide solution with a concentration of 3wt%. Add it to the above-mentioned starch-silver nitrate composite solution and mix evenly to obtain A ternary composite solution of silk fibroin peptide-starch-silver nitrate, adjusting the pH value of the solution to 9.2, and stirring at 70°C for 180 minutes to obtain a nano-silver hydrosol with a particle size of 3-25nm.

[0032] See attached figure 1 , which is the ultraviolet-visible absorption spectrum (UV-Vis figure) of the fibroin nano-silver hydrosol obtained in this embodiment, by figure ...

Embodiment 3

[0036]Disperse 0.1g of wheat starch in 10ml of deionized water, stir at 90°C until gelatinized to obtain a wheat starch solution, add it to 20ml of silver nitrate solution with a concentration of 0.1mol / L, mix well to obtain starch-silver nitrate binary compound solution. Take 1.2g of small-molecule sericin peptide with a molecular weight of 1-5KDa and the composite powder of small-molecule silk fibroin peptide and dissolve it in 60ml of deionized water to obtain a silk protein peptide solution with a concentration of 2 wt%, and add it to the above-mentioned In the starch-silver nitrate composite solution, mix evenly to obtain a ternary composite solution of silk protein peptide-starch-silver nitrate, adjust the pH value of the solution to 9.5, stir at 80°C for 120min, and obtain a nano-silver hydrosol with a particle size of 2-20nm .

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Abstract

The invention discloses a method for preparing fibroin nano-silver hydrosol. The method comprises the following steps of: dissolving or dispersing soluble amylose in deionized water, stirring the mixture until the amylose is completely gelatinized to obtain a starch solution, adding a silver nitrate solution, and then mixing the mixture with a fibroin solution to obtain a fibroin-starch-silver nitrate ternary complex solution; adjusting the pH value of the ternary complex solution to be at 8-10 by ammonia water, and fully mixing at the temperature condition of 20-80 DEG C to obtain the fibroin nano-silver hydrosol with the silver particle size being 1-100nm. According to the method, micromolecule fibroin peptide is used as a capturing agent and a reducing agent, the soluble starch is used as a dispersing agent and a protective agent, and the micromolecule fibroin peptide and the soluble starch are used for reducing and preparing nano-silver. The prepared nano-silver hydrosol is small in silver particle size, uniform in distribution and good in stability, in the prepared process, the reaction is mild, the operation is convenient, the green and environment-friendly aim is realized, and the fibroin nano-silver hydrosol can be widely used as a functional nano-material.

Description

technical field [0001] The invention relates to a preparation method of silk protein nano-silver hydrosol, which belongs to the technical field of nano-materials. Background technique [0002] Nano-silver is a single substance of metallic silver with a particle size at the nanometer level. Nano-silver materials have specific surface effects, small size effects, quantum size effects and macroscopic quantum tunneling effects, showing many strange physical and chemical properties, especially in electronics, optics, antibacterial and It has obvious performance advantages in catalysis and other aspects, and is widely used in catalyst materials, battery electrode materials, low-temperature heat-conducting materials, conductive pastes, antibacterial materials and medical materials, with broad prospects. [0003] Usually, the preparation methods of nano-silver are physical methods and chemical methods, among which the chemical method is widely used because the prepared nano-silver h...

Claims

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Application Information

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IPC IPC(8): B22F9/24
Inventor 林红陈宇岳熊佳庆
Owner SUZHOU UNIV
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