70 results about "Wheat starch" patented technology

The invention discloses a molecular marker remarkably related to the amylose content of wheat and application of the molecular marker, and belongs to the technical field of wheat breeding. Three mutation sites of wheat starch branching enzyme gene SBEIIa on 2A, 2B and 2D chromosomes and two mutation sites of SBEIIb on 2A and 2B chromosomes are identified, a KASP molecular marker system closely linked with SBEIIa and / or SBEIIb is developed based on the SNP sites, the detection process is simple and convenient, the cost is low, the throughput is high, and the application prospect is wide. According to the method, the high-amylose wheat material which contains the mutant genotype and can be stably inherited can be accurately and efficiently screened out, the breeding process of wheat variety quality improvement can be accelerated, and the method has important significance on creation of the high-amylose wheat material or molecular assisted breeding of the high-amylose wheat.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a TaMGD3 gene in improvement of plant starch quality and cultivation of a high-starch transgenic plant, including application of the TaMGD3 gene in promotion of wheat grain starch synthesis, application of the TaMGD3 gene in cultivation of the high-starch and / or high-amylopectin transgenic plant, and application of the TaMGD3 gene in improvement of plant starch quality and cultivation of the high-starch and / or high-amylopectin transgenic plant. The invention also discloses an application of the TaMGD3 gene in improvement of plant starch characteristics. A nucleotide sequence of the TaMGD3 gene is shown as SEQ ID NO. 1. The invention also provides a cultivation method of transgenic wheat. In wheat cultivation, wheat amylopectin quality improvement is realized through overexpression of the gene shown in SEQ ID NO.1. The TaMGD3 gene can improve the starch content of wheat grains, influence the particle size distribution of the wheat grain starch, improve the amylopectin proportion, improve the peak viscosity and the relative crystallinity of the starch, and facilitate the improvement of the cooking quality related to the wheat starch.

The invention relates to the technical field of aquaculture feed, in particular to babylonia feed and a preparation method thereof. The babylonia lutosa feed is prepared from the following raw materials: fish meal, shrimp meal, soybean meal, wheat starch, corn starch, fish oil, vegetable oil, sodium alginate, carrageenan, betaine, an amino acid mixture, a vitamin premix, a mineral premix and an antioxidant. The feed can be dried and stored at normal temperature, and is convenient to store and transport; before being used, the feed is soaked in clear water for 30 minutes and can be swelled and jellied, so that babylonia can eat conveniently; meanwhile, the zero-dissolution-loss characteristic is achieved, and pollution to water quality and water bottom is reduced; a good food calling effect is achieved, and the feeding rate of babylonia can be increased; moreover, eating safety is guaranteed, healthy growth of Babylonia lutosa is promoted, and breeding benefits are improved.

The application belongs to the technical field of soft capsules, and discloses a starch-based soft capsule material and a preparation method thereof. The preparation method comprises the following steps: mixing wheat starch and deionized water, adding a sodium periodate solution to react, so as to obtain dialdehyde starch; reacting the dialdehyde starch with glycidyl methacrylate and triethylamine, so as to obtain grafted modified starch; mixing the grafted modified starch with modified nano-silicon dioxide, and reacting after ultrasonic oscillation, so as to obtain nano-silicon dioxide composite modified starch; mixing the composite modified starch with polyvinyl alcohol and polyvinylpyrrolidone, so as to obtain a film forming solution; and after the film forming solution is cast and dried, hot pressing is performed, so that the starch-based soft capsule material is finally obtained. The starch is subjected to multiple hydrophobic modifications, so that the hydrophilic performance is reduced, and the stability of the soft capsule is improved.

The invention discloses low-digestibility animal protein-wheat flour composite noodles and a preparation method thereof, and belongs to the technical field of food processing. Wheat flour and exogenous animal protein are used as main raw materials, whey protein isolate, silkworm pupa protein, egg white protein and fish protein are selected, and the addition amount is 15% of the mass of the wheat flour. Through multi-index comprehensive screening, the silkworm pupa protein and whey protein isolate have better market development potential. By utilizing the synergistic effect of the exogenous animal protein and the wheat starch, the content of fast digestible starch (RDS) of the noodles can be remarkably reduced, the ratio of slow digestible starch (SDS) to resistant starch (RS) is increased, and the GI value of the noodles is lower than 70; meanwhile, cooking and texture characteristics are optimized, the cooking loss rate is smaller than or equal to 8%, and the breaking rate is smaller than or equal to 0.3%. The noodles have the characteristics of low glycemic content and high-quality eating taste, are balanced in nutrition, are suitable for diabetics to eat, can also be used as daily staple food of healthy people, and are simple and convenient in preparation process, easy to industrially produce and wide in market application prospect.

The invention belongs to the technical field of cultural relic protection, and particularly relates to a preparation method and application of a wheat starch adhesive suitable for repairing paper cultural relics. The preparation method of the wheat starch adhesive suitable for repairing the paper cultural relics comprises the following steps: S1, dissolving plant proteolytic enzyme in water, and uniformly stirring to prepare a plant proteolytic enzyme solution; s2, adding wheat flour into the vegetable protein hydrolase solution, and uniformly stirring; s3, further performing centrifugal separation, and washing, dehydrating and drying an obtained solid product for multiple times to obtain wheat starch; and S4, gelatinizing wheat starch to obtain the wheat starch adhesive. According to the method, the wheat flour is treated by adopting the vegetable protein hydrolase, so that protein combined with wheat starch can be efficiently removed, and the wheat starch with relatively high purity and dispersed particle morphology is released; and the formed wheat starch adhesive can significantly improve the folding resistance and tensile strength of paper in the repair of paper cultural relics.

This invention discloses a haplotype SNP molecular marker for identifying wheat grain starch content in the TaMYB44-4D gene and its application. Specifically, the SNP is T or C at position 1557bp of the sequence in SEQ ID NO.1. Based on this SNP, primers for detecting this haplotype SNP molecular marker have also been developed, and a kit containing these primers has been prepared. By PCR and polyacrylamide gel electrophoresis, the SNP and genotype can be accurately identified, thereby distinguishing between superior haplotypes (high starch content) and non-superior haplotypes (low starch content) in wheat.

The application relates to preparation of indoor potted solid water and belongs to the field of solid water preparation. A preparation method is as follows: nitrogen is introduced into a four-necked flask for 10 min to remove air, wheat bran and wheat B starch wastewater are added, heating and stirring are carried out, sodium alginate and gellan gum are added to prepare A liquid; acrylic acid is neutralized by adding 25% NaOH solution under the condition of an ice water bath, after the neutralized liquid is cooled to room temperature, 2-acrylamide-2-methylpropanesulfonic acid, bentonite, methylene bisacrylamide and potassium persulfate are added to prepare B liquid; B liquid is slowly added into A liquid, heating and stirring are carried out to perform polymerization reaction, after the reaction is completed, filtration is carried out, anhydrous ethanol and distilled water are used for washing, and indoor potted special solid water is obtained. The indoor potted solid water provided by the application can provide convenience for office workers to maintain potted plants, can improve soil permeability and water absorption and water retention capacity, has a long use period, and is prepared by using natural degradable materials and has low cost.

The invention discloses a preparation method of soluble modified starch, the soluble modified starch and an application of the soluble modified starch in resisting caking, the method comprises the following steps: preparing wheat starch or corn starch into starch slurry, and then carrying out acidolysis reaction to obtain wheat starch or corn starch after acidolysis; the wheat starch or the corn starch after acidolysis is prepared into starch slurry, the starch slurry is subjected to an enzymatic hydrolysis reaction to obtain an enzymatic hydrolysis solution, the enzymatic hydrolysis solution is subjected to spray drying to obtain the soluble modified starch, and the soluble modified starch can be used for resisting caking and improving the caking resistance. According to the method, the solubility of the wheat starch or the corn starch can be remarkably improved, the application range of the wheat starch or the corn starch is widened, and the method has important practical significance and economic value.

An emulsified composition containing methyl cellulose, a starch material, edible oil or fat, and an emulsifying material containing protein. The starch material is a component (A) which is a powdery material satisfying (1) a starch content is 75% by mass or more, (2) 3-45% by mass of a low molecular weight starch (a peak molecular weight of equal to or more than 3×103 and equal to or less than 5×104) including 5% by mass or more of an amylose content, (3) a degree of swelling in cold water at 25° C. is 5-20 and (4) a content under a sieve with openings of 3.35 mm and on a sieve with openings of 0.038 mm is 60-100% by mass; and / or a component (B) a raw material starch of which is a cornstarch, a tapioca starch, a wheat starch, and / or a pea starch.

The invention discloses application of a TaSMAX1 protein and a coding gene thereof in improving the gelatinization characteristic of wheat starch, and belongs to the technical field of biology. The protein is shown as SEQ ID NO: 2, and the coding gene of the protein is 58th to 3123th sites of SEQ ID NO: 1. Experiments prove that compared with receptor wheat Fielder, the stability index breakdown value and low ebb viscosity of TaSMAX1 protein overexpressed strain starch are obviously improved, the gelatinization temperature is reduced, the food processing performance is improved, and it is proved that overexpressed TaSMAX1 protein can effectively improve the gelatinization characteristic of wheat starch. The invention provides an excellent gene resource and a technical path for cultivating high-yield and high-quality wheat varieties, and has a wide application prospect.

The present application relates to a method for preparing Spanish fried bread sticks, comprising: a first step of obtaining a composition for preparing Spanish fried bread sticks, the composition comprising flour, wheat starch and methylcellulose; a second step of preparing a dough by kneading the composition for preparing Spanish fried bread sticks with hot water; step 3, shaping the prepared dough; and a fourth step of performing heat treatment on the molded dough.

The invention discloses gluten-free clam crackers and a preparation method thereof, and belongs to the field of food processing. The gluten-free clam crackers comprise the following basic raw materials in percentage by mass: 50-60% of clam freeze-dried powder and 40-50% of wheat starch. And the auxiliary materials comprise the following components in percentage by mass (calculated by the basic raw materials): 5-25% of vegetable oil, 0-0.25% of salt and 150-400% of water. The biscuit is rich in high protein, low fat, various vitamins, calcium, phosphorus, iron, selenium and other nutrient substances required by the human body, has good brittleness, good taste and good appearance, can meet the requirements of consumers, is simple in preparation process and low in production cost, can occupy an important position in the snack food market, and is suitable for large-scale processing and production.

The invention provides an acidic decolorizing agent. The invention relates to an adhesive which comprises the following components in parts by weight: 30-34 parts of hydrolyzed wheat starch, 28-32 parts of wheat flour, 12-16 parts of potassium persulfate, 4-8 parts of ammonium persulfate, 3.5-7.5 parts of hydroxyethyl cellulose, 1.5-5.5 parts of silicon dioxide, 1-2 parts of hydroxypropyl trimethyl ammonium chloride hydrolyzed collagen, 0.8-1.3 parts of guar gum and 0.8-1.3 parts of sodium metasilicate. The invention relates to an anti-rust agent, which is prepared from the following components in parts by weight: 0.8 to 1.3 parts of magnesium stearate, 0.8 to 1.3 parts of magnesium carbonate, 0.8 to 1.3 parts of disodium ethylene diamine tetraacetate, 0.08 to 0.12 part of hydrolyzed keratin, 0.4 to 0.8 part of sodium laureth sulfate, 0.3 to 0.5 part of titanium dioxide, 0.3 to 0.5 part of glucose and 0.1 to 0.3 part of mineral oil.

The invention discloses a KASP molecular marker for identifying wheat starch gelatinization characteristics and application of the KASP molecular marker, and an SNP site corresponds to a 4D chromosome physical position 24260827bp and comprises two allele types, namely a T type and a G type. The composition for identifying or assisting in identifying the wheat starch gelatinization characteristic is developed on the basis of the SNP site, a method for detecting or assisting in detecting the wheat starch gelatinization characteristic is provided, wheat germplasm resources with the high peak viscosity characteristic can be efficiently and rapidly screened, and the breeding process of new high-quality wheat varieties is remarkably accelerated.

The invention belongs to the technical field of starch deep processing, and particularly relates to a preparation method of functional high-resistance starch puffed food, which comprises the following steps: S1, modifying wheat starch by adopting physical microwave heat-moisture treatment; s2, preparing probiotic microcapsules by adopting a spray freeze-drying composite embedding method; s3, 3D printing pretreatment: mixing the resistant wheat starch, the embedded probiotic mixture, sodium carboxymethyl cellulose and glycerol, adding liquid carbon dioxide, and stirring at the temperature of 10 DEG C below zero to 5 DEG C below zero to form uniform viscous slurry; s4, 3D printing forming is conducted; s5, drying treatment; s6, seasoning; and S7, naturally cooling the seasoned materials, and packaging the seasoned materials to obtain a final product. According to the puffed food prepared through 3D printing and liquid carbon dioxide non-thermal puffing technologies, the problems that the content of resistant starch is reduced and heat is increased due to a traditional extrusion puffing technology are thoroughly solved.

Disclosed are a food grade starch based modeling clay with high elasticity and high ductility and its preparation method, belonging to the technical field of modeling clay preparation. The starch based modeling clay provided by the present disclosure is prepared from the following formula components: 160-180 parts of starch, 140-160 parts of water, 10-15 parts of a food grade stabilizer, 10-15 parts of edible salt, 0-10 parts of hydrophilic colloid, 0.6-0.8 parts of a preservative, 40-60 parts of a food grade plasticizer, 6-10 parts of food grade white oil, and 4-8 parts of edible pigment. The starch includes one or a combination of more of cassava starch, wheat starch, and glutinous rice starch. The present disclosure, through combined use of the starch and the hydrophilic colloid, makes the prepared starch based modeling clay have good viscoelasticity and ductility, and good in storage stability, safe and non-toxic.

The invention discloses prepackaged fresh-keeping wet rice noodles with a normal-temperature shelf life of 12 months and a preparation method of the prepackaged fresh-keeping wet rice noodles. 30-90% of early indica rice and 70-10% of corn starch or wheat starch are used as ingredients, so that the total content of amylose in the mixed raw materials is greater than or equal to 21.5%; adding lactic acid bacteria, and performing constant-temperature and constant-humidity solid-state Steaming the extruded rice vermicelli for more than two times; soaking the acidified vermicelli with lactic acid drinking water; softening and acidifying the vermicelli with hot water at 59-70 DEG C; and weighing, bagging, packaging, sterilizing, cooling and warehousing. The rice noodles are safe in microorganism and physicochemical indexes, are not naturally broken, and are quick and convenient to brew / cook; the preparation method enhances the toughness of the vermicelli, and the vermicelli is not easy to break; steaming for multiple times to inhibit retrogradation; through three high-temperature processes of squeezing, steaming and high-temperature sterilization, the prepared rice noodles can resist high temperature; the sterilization is thorough, and the shelf life is longer.

The application provides a preparation method of anti-aging wheat starch; high-temperature-resistant alpha-amylase, papain and saccharifying enzyme are used for enzymolysis of young leaves of highland barley; the product after the enzymolysis is subjected to first solid-liquid separation, and residues are collected; the residues are added with xylanase and cellulase for enzymolysis; the product is cooled to room temperature and subjected to second solid-liquid separation; polysaccharide in the liquid is precipitated by ethanol; the precipitate is redissolved, dialyzed, concentrated by rotary evaporation and vacuum freeze-dried to obtain polysaccharide; the polysaccharide is dissolved in water to obtain a uniform polysaccharide solution; wheat starch is added into the polysaccharide solution; the mixture is stirred and uniformly mixed to obtain a mixed system; the mixed system is heated and gelatinized; the product is obtained by cooling at room temperature and freeze-drying; and the anti-aging wheat starch prepared based on the polysaccharide obtained from the enzyme residue can be used for making various snacks, such as crystal moon cake crust, shrimp dumplings, powder cakes, rice rolls and the like, and can enhance the anti-aging function of food.

The invention discloses a molecular marker closely linked with wheat starch value major QTL-qWSd-4D and application of the molecular marker, and belongs to the technical field of wheat molecular biotechnology and breeding. The molecular marker is WSD-ID-29.9 and is closely linked with wheat starch value major QTL located on a wheat 4D chromosome, the nucleotide sequence of the molecular marker is shown as SEQ ID NO: 3 or SEQ ID NO: 4, and the molecular marker can be obtained by amplifying primer pairs shown as SEQ ID NO: 1 and SEQ ID NO: 2. The molecular marker WSD-ID-29.9 fully reflects the starch (dry basis) value main effect QTL of the wheat variety (line), whether the wheat variety (line) contains the starch (dry basis) value main effect QTL or not can be rapidly and accurately judged, and an excellent gene resource and a selection tool are provided for wheat starch (dry basis) value character molecular breeding.

The invention provides a method for preparing edible alcohol by fermenting wheat starch, which comprises the following steps: pretreatment, enzymolysis, composite flora fermentation and purification. The pretreatment method comprises the following steps: sieving wheat B starch with a 90-110-mesh sieve, adding water to prepare 32wt%-35wt% starch milk, adding a composite pre-oxidant, carrying out heat preservation for 40-50 minutes under the conditions that the temperature is 46-50 DEG C and the pH value is 4.9-5.2, then adding a composite degrading enzyme, and carrying out heat preservation for 20-25 minutes, so as to obtain pretreated starch milk; the method finally realizes double improvement of product quality and production benefit, the viscosity of the pretreated starch milk is 270-300 cP, the reducing sugar of the converted mash is 213-232 g / L, the fermentation time is 48-52 h, the residual total sugar of the fermented mature mash is 1.5-1.7 g / L, the purity of the prepared alcohol reaches 96.3%-96.6%, the yield is 0.459-0.466 L / kg starch, the content of n-propyl alcohol is 0.2-0.4 mg / L, and the content of isobutanol and isoamyl alcohol is 0.3-0.6 mg / L.

The invention discloses a preparation method of wheat B starch slurry fermented milk, and relates to the technical field of dairy product processing. The method comprises the following steps: after boiling and cooling wheat B starch slurry, sequentially carrying out high-temperature-resistant alpha-amylase enzymolysis, compound enzyme enzymolysis, enzyme deactivation and filtration to obtain wheat B starch slurry enzymolysis saccharification liquid; uniformly mixing the enzymolysis saccharification liquid with fresh milk and cane sugar to prepare a blending liquid; adding compound zymophyte into the blended liquid for fermentation to obtain initial fermented milk; adding a compound stabilizer, performing high-pressure homogenization and ultrahigh-pressure instantaneous sterilization, and performing sterile canning, so as to obtain the wheat B starch slurry fermented milk. According to the method, the problem of difficulty in saccharifying the wheat B starch slurry is effectively solved through a step-by-step enzymolysis process, and high-value utilization of industrial byproducts is realized; the obtained fermented milk is rich in nutrition, good in stability, green and environment-friendly in preparation process and suitable for industrial popularization.

The invention provides a compound enzyme composition and application thereof. The compound enzyme composition contains low-temperature amylase and glucoamylase with the activity ratio of (0.8-1.2): (0.8-1.2), wherein the amino acid sequence of the low-temperature amylase is as shown in SEQ ID NO: 1. The compound enzyme composition can adapt to starch with complex crystal structures such as corn starch, rice starch and wheat starch, the prepared porous starch can serve as a slow-release carrier, an embedding material is efficiently loaded in a pore structure through the excellent adsorption characteristic and slow-release characteristic of the porous starch, the embedding material is continuously released in an acting part or an acting environment, and the slow-release effect is achieved. Efficient embedding and effective slow release of the embedding material are achieved, and the method is suitable for loading the embedding material such as active substances or flavor substances in products such as drugs, food and health-care products.

The invention relates to the technical field of development of active peptides, in particular to a novel small-molecule heptapeptide with a starch digestion inhibiting effect and application thereof. According to the method, perigord truffle is taken as a raw material, and the perigord truffle polypeptide is obtained through protein extraction, protein enzymolysis, dialysis and freeze-drying. Furthermore, a bioinformatics tool is used for predicting the activity, potential toxicity, solubility and physicochemical properties of the peptide fragment, wheat starch is selected as a test material, the digestion characteristic of the polypeptide-starch compound is detected, and the peptide fragment is determined according to the starch hydrolysis rate and the ratio of rapidly digested starch to slowly digested starch to resistant starch. The heptapeptide AADELFR with the effect of inhibiting or delaying starch digestion is obtained and is applied to preparation of food or health food beneficial to maintaining the healthy level of blood sugar.

The application belongs to the technical field of food processing, and particularly relates to a processing method of wheat B starch-based instant vegetarian sausage. The processing method comprises the following raw materials in parts by weight: 80-90 parts of homogenate, 6-8 parts of glue solution, 8-10 parts of salt, 8-10 parts of coconut oil, 4-6 parts of monosodium glutamate, 4-6 parts of red yeast powder, and 2-4 parts of tripolyphosphate; the preparation method of the instant vegetarian sausage comprises the following steps: preparing homogenate, preparing glue solution, modulating, sausage filling, and steaming and cooking. The application adds wheat B starch slurry and compounding specific proportions of composite colloids, utilizes the synergistic water holding effect of arabinoxylan and colloids, and gives the vegetarian sausage excellent elastic and tough mouthfeel and juiciness. The application converts low-value wheat B starch slurry into raw materials of instant vegetarian sausage, completely retains the arabinoxylan dietary fiber therein, makes the product have both nutritional function and industrial value, and significantly improves the added value of by-products.

The invention belongs to the technical field of soft capsule shells, and particularly relates to a plant capsule shell composition and a preparation method thereof. The potato starch is subjected to enzymolysis modification through beta-1, 3-glucanase and pectinesterase, the disadvantage that natural starch is too large in viscosity is overcome, the potato starch is compounded with the hydroxypropyl starch and the wheat starch, the starch viscosity is effectively reduced, meanwhile, the gelatinization characteristic is improved, and gel is rapidly formed; the fluidity of the glue solution meets the requirements of pelleting equipment, and the disintegration performance is stable; aspergillus niger saccharifying enzyme and beta-glucanase are used for carrying out enzymolysis modification on the plant powder, and then the plant powder is mixed with hydroxypropyl methyl cellulose, so that a tough gel network of the plant capsule is facilitated, the rubber binding rate is increased, the oil leakage problem of the plant capsule is reduced, and the integrity of the capsule is improved. Therefore, the technical scheme provided by the invention is simple and convenient to operate, and the obtained rubber is high in integrity and stable in disintegration performance.

This invention discloses a high-viscosity, high-toughness wheat starch-based paperboard adhesive and its preparation method. The adhesive uses wheat starch as the main raw material, which is divided into cooked wheat starch and raw wheat starch. The cooked wheat starch undergoes slight esterification modification, is mixed with a film-forming aid and gelatinized, and then a rigid micro-crosslinking agent is added to form a cooked adhesive carrier system. The raw wheat starch is dispersed with an alkaline regulator at a constant temperature of 25±2℃ to form a raw adhesive matrix system. After mixing the cooked adhesive carrier system and the raw adhesive matrix system, a flexible dynamic crosslinking agent is added when the system is cooled to 30-40℃, and the adhesive is then subjected to pH adjustment, filtration, and maturation to obtain the finished product. This invention improves the problems of low viscosity, strong settling, and brittle adhesive film of wheat starch adhesive solution under conditions of lower alkali and lower modifier dosage, thereby improving the bonding strength, storage stability, and adhesive layer toughness of paperboard.

The application belongs to the technical field of polymer materials, and discloses a design method of multifunctional hydrogel based on a rare earth-starch system.The hydrogel specifically comprises starch (potato starch / wheat starch / corn starch), acrylamide, polyvinyl alcohol, silver nanowires and europium ions.The hydrogel has a fluorescent and conductive double channel, overcomes the disadvantage of traditional rare earth hydrogel which can only be used as a fluorescent sensor, and provides a stronger network by physical crosslinking of the rare earth-starch, so that the mechanical properties are enhanced; meanwhile, the hydrogel can freely adjust the mechanical properties and the fluorescent intensity by changing the starch content and respond to the external ion environment, and has a very high sensing sensitivity (GF = 9.02) and mechanical properties (strain = 1377%); the dynamic response time can reach 52 ms.The hydrogel sensor has stable performance and is convenient to prepare, widens the application range of the rare earth hydrogel, and has a good development prospect.

The invention discloses a dsRNA fragment capable of improving wheat amylopectin content and a synthesis method and application thereof, and relates to the field of wheat starch content regulation, pET28a is used as an initial skeleton, a sequence containing a reverse T7 promoter, multiple cloning sites and ISA1 or ISA2 gene fragment is inserted, a recombinant vector is constructed through restriction enzyme digestion connection of restriction enzymes SalI and EcoRI, and the recombinant vector is used as a recombinant vector for improving wheat amylopectin content. And transforming the recombinant expression vector into an escherichia coli HT115 competent cell, and synthesizing the dsRNA fragment after induced expression. According to the invention, a non-transgenic transient silencing technology is adopted, and target gene expression is inhibited in a targeted manner, so that the problems of long period, low efficiency, dependence on complex gene cloning and vector construction processes and potential off-target effect and ecological risk existing in the traditional gene technology are avoided.

The present application relates to the technical field of food 3D printing, and discloses a method for determining 3D printing parameters of non-starch polysaccharide in starch-based food, astragalus polysaccharide composite gel and a preparation method thereof, and a method for determining optimal printing parameters, which comprises preparing gel material for 3D printing, and the preparation raw material of the gel material comprises non-starch polysaccharide and starch, the gel material is sent into a 3D printer for printing, and the influence of different printing speeds, filling rates and nozzle diameters on the quality of the formed sample is studied through single-factor test, the quality of the formed sample is evaluated based on comprehensive printing accuracy and quality index, and the optimal values of the printing speed, the filling rate and the nozzle diameter are determined, the present application provides a method for quickly determining 3D printing parameters of non-starch polysaccharide in starch-based food, and uses astragalus polysaccharide and wheat starch as raw materials, so that the strength of the gel material can be effectively improved, the printing accuracy of the formed product can be improved, and problems such as difficulty in internal refinement of the gel material are effectively solved, and the preparation method is simple, low in cost and short in time consumption.