The invention belongs to the field of biological refining of
grease, and particularly relates to a
grease degumming method based on a
phospholipase micromotor reactor. The invention discloses an enzymatic degumming method of edible
vegetable oil, algal oil and
animal fat, which aims at the pain points of low degumming efficiency, long time consumption, easy
enzyme inactivation and difficult
recovery of the traditional enzymatic degumming, designs core-shell
mesoporous silica microspheres, adopts an
emulsion method to respectively graft
alkyl and alkylamine on two sides of the microspheres, constructs a Janus carrier with asymmetric chemical environment, and improves the degumming efficiency of the edible
vegetable oil, the algal oil and the
animal fat. And immobilizing
phospholipase A1, A2, B or C through physical adsorption to prepare the
phospholipase micromotor reactor. In the degumming process, a
micromotor cooperates with an interface activation-enhanced
mass transfer-dynamic self-cleaning mechanism, self-driven movement in an interface micro-scale range is realized by virtue of a
substrate concentration gradient, the
enzyme activity and stability of phospholipase are remarkably improved, and efficient degumming of
grease is realized. The method is high in oil yield, green in process and suitable for industrial popularization.