33 results about "Black sea bream" patented technology

The invention discloses a black sea bream antibacterial peptide AS-hepc3 (48-56) and application thereof. The molecular formula of the black sea bream antibacterial peptide AS-hepc3 (48-56) is C48H86N24O10S3, and the amino acid sequence of the black sea bream antibacterial peptide AS-hepc3 (48-56) is SEQ ID NO: 01. The antibacterial peptide AS-hepc3 (48-56) disclosed by the invention is applied asan effective component of an antibacterial drug, has the characteristics of small length, high antibacterial activity, difficulty in generating drug resistance, capability of resisting various clinical drug-resistant bacteria and the like, and has a wide prospect in the aspect of research and development of drugs for resisting drug-resistant bacteria.

The invention relates to a rapid extraction method of black sea bream genome DNA. The method includes the steps of grinding or shearing a black sea bream fine ray or muscle sample, adding the material to an extraction buffer solution to be vibrated and evenly mixed to obtain a black sea bream tissue extraction solution, putting the solution at 65 DEG C for 20-40 min, taking out a sample, and conducting cooling, standing still or centrifuging to obtain supernate to obtain the black sea bream genome DNA. The method is simple in extraction step, rapid and low in requirements for experiment instruments, the extraction of the genome DNA can be completed within 1 hour, and the method is more rapid and has good application prospects compared with an extraction method using a kit.

A method for constructing a model for enhancing the immune mechanism of black sea bream by studying selenized aminopolysaccharide based on liver metabolomics, relates to the field of biomedical analysis, and includes the following steps: 1) dividing black sea bream juveniles into an experimental group and a blank group equally; 2 ) Selenized amino polysaccharide was added to the feed of the experimental group; 3) The juvenile black sea bream in the experimental group and the blank group were starved, and after anesthesia, the liver tissue was taken out and stored for future use; 4) The liver tissue samples were prepared and the quality Control samples were collected and processed using ultra-high performance liquid chromatography-time-of-flight mass spectrometry; 5) The collected metabolomic data of the liver of black sea bream were analyzed and processed to identify and screen out the information on the enhanced immune mechanism of selenized aminopolysaccharides on black sea bream. liver metabolomic biomarkers, and construct and analyze the metabolic pathways pointed to by the liver metabolomic biomarkers. The present invention has good systematicness, high accuracy, low cost and simple operation.

The hybrid breeding technology of freshwater black sea bream and gem perch belongs to the field of breeding. The hybrid breeding technology of freshwater black sea bream and gem perch is as follows: parent selection → parent cultivation → parent selection → artificial induction → parental male and female separate ponds for temporary production → artificial insemination → cleaning fertilized eggs → counting fertilized eggs → artificial hatching → fry hatching → Fry breeding. After the broodstock enter the pond, a series of measures of insecticide, antibacterial and antifungal are used to prevent and control fish diseases, wheat germ is fed, the bottom is improved with a substrate improver, and HCG + high-efficiency No. 3 fish oxytocic agent is used to induce labor. After injecting the oxytocic agent, the male and female broodstock are kept in separate pools for temporary breeding. The present invention carries out crossbreeding of freshwater black sea bream and gem perch. The overall performance index reaches 80‑100% of induced spawning rate, 80‑100% of fertilization rate, and 30‑100% hatching rate of fertilized eggs. 95%.

The invention relates to a method for prolonging the growth period of black sea bream. A salinity regulation manner is adopted to improve the temperature tolerance capability of the black sea bream, and thereby prolonging the growth period of the black sea bream, so that the method is easy to operate and strong in feasibility.

The invention relates to a method for fish crossing semination, which in detail is a method for crossing semination between red porgy and black porgy. The method comprises following steps: collecting healthy and sexual mature red porgy as female parent, collecting healthy and sexual mature black porgy as male parent; putting ovum of red porgy into beaker, which is high quality, putting sperm of black gorgy into said beaker; shaking lightly said beaker, adding into filtered sea water to activate sperm; shaking or stirring to make ovum and sperm contact thoroughly; adding filtered seawater and stewing; washing ovum with net sheet, putting it into seawater for stewing; putting floated fertilized ovum into hatching net cage filled with seawater, and aerating for hatching. The invention is characterized by simple operation, low cost, and high efficiency; the fish combines the sound property of parent and is characterized by fast growth speed, strong disease resistance and good adverse resistance.

The present invention discloses primers, kit and identification method for identifying Pagrosomus major, Acanthopagrus schlegeli and their hybrids, wherein the kit includes the primers, a buffer solution, enzymes and the like, and the identification method includes: first, acquiring a snapper sample to be identified, and extracting genome DNA; second, using the genome DAN as a template to carry out PCR (polymerase chain reaction) amplification; third, subjecting an amplified product to enzyme restriction and electrophoresis, and identifying according to characteristic bands. The primers, kit and identification method help quickly and accurately identify Pagrosomus major, Acanthopagrus schlegeli and their hybrids and are of great application value in snapper germplasm identification, breed conservation and selective breeding.

The invention belongs to the field of marine fish farming, and particularly relates to a marine fish black porgy overwintering feed. The marine fish black porgy overwintering feed is prepared from the following components in parts by weight: 20-25 parts of white fish powder, 15-20 parts of soybean meal, 15-20 parts of fermented soybean meal, 5-10 parts of shrimp base powder, 15-20 parts of high protein flour, 6-8 parts of fish oil, 6-8 parts of corn oil, 3-5 parts of sleeve-fish ointment, 1-3 parts of carboxymethylcellulose, 1-2 parts of soyabean lecithin, 1-2 parts of sodium dihydrogen phosphate, 0.01-0.50 part of vitamin C, 0.03-0.05 part of vitamin E, 0.5-1 part of multi-vitamins, 0.5-1 part of complex mineral substances, 0.4-0.8 part of choline chloride, 0.4-0.8 part of glycine betaine, 0.01-0.20 part of allicin, 0.01-0.20 part of curcumin and 0.1-0.3 part of a complex enzyme preparation by virtue of mixing. Contents of crude fat and unsaturated fatty acid in the feed disclosed by the invention are moderate, thus the demand on growth of black porgies during overwintering farming is met, the ingestion of the black porgies is favorably promoted, and the survival rate of the black porgies is increased.

The invention discloses a Chinese herbal medicine preparation for preventing and treating the skin ulcers of the industrially bred black sea breams. The Chinese herbal medicine preparation comprises, by weight, 12-22 parts of oriental wormwood, 16-20 parts of the bupleurum, 12-18 parts of radix sophorae flavescentis, 8-12 parts of isatis root, 12-18 parts of coxtex moutan, 12-18 parts of polygonum cuspidatum, 2-6 parts of perilla, 8-11 parts of honeysuckle, and 2-6 parts of licorice. The traditional Chinese medicine is reasonably formulated, and in the aquiculture industry, provides effective treatment medicine for the industrially bred black sea breams that is anti-bacterial anti-inflammatory, enhances the effectiveness of immunity and has the advantages of fast effectiveness, solid curative effect and no drug residue and toxic and side effects. The application of the Chinese herbal medicine preparation of the invention can effectively control the common diseases of the industrially bred black sea breams and reduce the morbidity and mortality of common diseases.

The invention relates to a method for using microsatellite markers to identify hybrid seabream families, belonging to the field of molecular markers. Firstly, the tail fin samples of the female red sea bream and male black sea bream and their hybrid offspring were cut, and the DNA was extracted, and then the DNA of the parents and the hybrid offspring were used as templates, and microsatellite marker primers were used for multiplex PCR amplification; The products were subjected to polyacrylamide gel electrophoresis, analyzed with BandScan5.0 software, combined with the length of PCR products to determine the size of alleles, and determined individual genotypes, and established genotype data files of hybrid seabream and parents according to the analysis results; using Cervus3.0 software Perform family identification. The present invention adopts microsatellite marker technology to distinguish male and female parents and offspring genotypes for hybridization on 10 microsatellite loci, can accurately and conveniently determine the family source of offspring of hybrid sea bream, and quickly and efficiently select and breed excellent hybrid sea bream family, It brings great convenience to genetic breeding work.

The invention relates to the field of seawater fish farming, in particular to a seawater black bream feed with immune enhancing effect, comprising fish meal, fermented soybean meal, cornstarch, corn oil, disodium hydrogen phosphate, Antarctic krill powder, krill oil, compound Vitamins, compound Chinese herbal medicine, compound seaweed powder, compound minerals and waterworm protein powder. Each component is formed with fish meal and fermented soybean meal as the main material, corn starch, corn oil, disodium hydrogen phosphate, complex vitamins and complex minerals as auxiliary materials, Antarctic krill powder and krill oil as the main enhancer, and compound Chinese herbal medicine and compound seaweed powder are the reasonable compatibility of the auxiliary enhancer, and the water silk worm protein powder is used to further enhance food attraction and immunity. The proportion of each component is appropriate, which can enhance the body immunity of black snapper and the antibacterial, antibacterial and antibacterial properties of black snapper. It has anti-virus and anti-infection functions, and has good food-attracting effect, low bait coefficient, and has wide applicability to aquaculture fish.

The present invention introduces a tomato juice and black sea bream can and a preparation method thereof, belongs to the field of food processing. The tomato juice and black sea bream can is achieved by the following processes: choosing the preferable black sea bream, selecting materials, washing and cleaning the black sea bream, then drying, slicing and pickling the black sea bream, sterilizing the black sea bream with high-temperature steam, dehydrating the black sea bream, soaking the black sea bream by pouring soup, mixing the black sea bream with drinking water, vacuum-sealing the black sea bream, and stationarily storing the obtained end product of the black sea bream. The processes of the tomato juice and black sea bream can are designed in reason, which make the can rich in nutrient, bright red in color, sweet and sour and tasty in taste, rich in tomato flavor, and convenient to carry, eat and preserve.

The invention provides a seawater fish black bream net cage overwintering culture method, which belongs to the field of aquaculture technology. The black bream is stocked in the net cage placed in the sea, and after the taming is successful, the compound feed containing asparagus fermented product is fed, and the asparagus is fed. The fermented product contains high F value oligopeptides and active agar oligosaccharides, and the preparation method of the asparagus fermented product is: according to the inoculum size, it is 10-12% to contain 4-5wt% asparagus dry algae powder, pH 7.0- Inoculate Bacillus subtilis into the solution of 8.0, add sucrose acetate isobutyrate and hesperetin, ferment, dry, and sieve to obtain asparagus fermented product. The overwintering breeding method of the invention can make full use of the water body breeding area, enhance the physical fitness of the overwintering black sea bream, improve its anti-stress ability and immune and anti-oxidation ability, improve the survival rate, and survive the winter safely.

The invention belongs to seawater fish cultivation technique, and particularly relates to an overwintering cultivation method of a seawater fish black sea bream net cage. The method specifically comprises the following steps of A, selection of a sea area, that is to say, a seawater quality index conforms to an NY5052-2001 water quality standard of seaculture of pollution-free food, the salinity is 2-3%, the pH value is 7.8-8.6 and the dissolved oxygen is more than 5 mg / L; when the water temperature of the sea area rises to more than 15 DEG C in the following spring, a black sea bream cultivation net cage is slowly hauled back to an open sea area to cultivate; B, selection of the net cable and cultivation density, that is to say, the overwintering cultivation net cage selects a conventional small net cage or a deep water net cage, a plurality of net cages form a net row type structure and are arranged in parallel along the downstream direction; during overwintering cultivation, the cultivation density of the black sea bream net cage is 8-10 g / cm<3>; C, feed selection; D, a feeding method of a feed, that is to say, feeding is not carried out under the conditions of the water temperature in winter of lower than 10 DEG C and rainy days, feeding is carried out at sunny days at more than 10 DEG C, satiation feeding is carried out at 13:30 to 15:30 at one time, and the feeding rate is controlled within 1%-2%. According to the method, successful overwintering of cultivation of the black sea bream net cage is implemented through management of the cultivation method and feed improvement.