SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of black sea bream and application of SNP molecular marker

A technology of molecular markers and growth traits, which is applied in the field of molecular biology, can solve the problems of black sea bream parent quality, large growth disparity, cannibalism and other problems, so as to shorten the breeding cycle, speed up the breeding process and improve the accuracy Effect

Pending Publication Date: 2022-05-06
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is convenient, fast and simple, but it is greatly affected by human factors. In addition, black sea bream is a carnivorous fish with strong predatory nature. If the bait is insufficient, it will eat each other, resulting in a large difference in growth.
Therefore, judging the parental quality of black sea bream only from the phenotype often does not achieve the desired effect

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of black sea bream and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of black sea bream and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of black sea bream and application of SNP molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Acquisition of SNP molecular markers

[0030]The experimental fish used were the same batch of newly hatched black sea bream, cultivated in outdoor ponds, and fed with feed twice a day. During the cultivation period, the water temperature and salinity were within the adaptable range of black sea bream cultivation. At 4 months of age, the survival rate of black sea bream is 100%. Select 5 extremely large individuals (fast growth) and 5 extremely small individuals (slow growth) from all black sea breams, cut tissue samples immediately after anesthesia, then extract the total RNA of each fish, and take an equal amount of total RNA The RNA libraries of the fast-growing group and the slow-growing group were constructed, and transcriptome sequencing was performed. The sequencing results were compared with the whole genome sequence of black sea bream. After analysis by bioinformatics software, a large number of potential SNP sites were obtained, among which adenylate kinas...

Embodiment 2

[0044] Utilize the SNP molecular marker that embodiment 1 screens out to screen the black sea bream parent with high growth rate, comprise the steps:

[0045] (1) Cut the black sea bream caudal fin sample to be tested, extract DNA;

[0046] (2) Using the extracted DNA as a template, the primer pair is used to perform PCR amplification to obtain the primary PCR amplification product;

[0047] Among them, the primer pair for detecting the SNP molecular marker located in the adenylate kinase 2 gene is:

[0048] F1: 3'-ACGTTGGATGTCGTGGAGCTCATAGAGAAG-5' (SEQ ID NO.4)

[0049] R1: 3'-ACGTTGGATGTCTCCGCTTGTTTGACTGTG-5' (SEQ ID NO.5)

[0050] Extension primer 1: 3'-AGAAGAACTTGGACACGTC-5' (SEQ ID NO.6);

[0051] The primer pair used to detect the SNP molecular marker located in the cytochrome P4503A gene is:

[0052] F2: 3'-ACGTTGGATGTACCCTTTGGAACCACAAGC-5' (SEQ ID NO.7)

[0053] R2: 3'-ACGTTGGGATGTTTGTACCCCATCCTTCCAC-5' (SEQ ID NO.8)

[0054] Extension primer 2: 3'-CACAAGCCCATTTA...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of black sea bream. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3. The invention further discloses an application of the SNP molecular marker. The 276th basic group from the 5'end of the SEQ ID NO.1 is T or C, and the growth speed of an individual with the genotype of TT is obviously higher than that of an individual CC and an individual TC; the 364th basic group from the 5'end of the SEQ ID NO.2 is A or C, and the growth speed of individuals with the genotype of AA is obviously higher than that of CC and AC individuals; the 6070th basic group from the 5'terminal of the SEQ ID NO.3 is A or T, and the growth speed of an individual with the genotype of TT is obviously higher than that of an AA individual and an AT individual. The three SNP molecular markers found by the invention can be used for rapidly obtaining a black sea bream individual with high growth speed and genetic stability, and can be applied to breeding a black sea bream variety with rapid growth.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP molecular marker related to the growth traits of black sea bream and its application. Background technique [0002] Black sea bream (Acanthopagrus schlegelii), also known as Heijiaji, Heili, sea bream, etc., belongs to the order Perciformes, the family Sparidae, and the genus Acanthopagrus. reef fish. It mainly lives in the coastal areas of the western Pacific Ocean, and is distributed in the north and south sea areas of my country. It has the advantages of strong stress resistance and fast growth, and is one of the important marine culture species in my country. At present, the black sea bream cultivated in my country is domesticated from wild species, lack of directional selection, and no black sea bream strain with excellent growth rate has been formed. Due to the fact that there are relatively few diseases in black sea bream farming, the market p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2521/525C12Q2531/113C12Q2533/101C12Q2565/627
Inventor 张志伟林志杰张志勇卫明亮祝斐贾超峰孟乾徐大凤陈淑吟张曹进
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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