100results about How to "Shorten the breeding cycle" patented technology

The invention belongs to the technical field of cotton molecular breeding, and discloses SNP molecular markers associated with fiber strength of upland cotton as well as detection of the SNP molecular marker and application of the SNP molecular marker. The SNP molecular marker takes a cotton stable RIL group as a material, and is obtained through a genome re-sequencing method. The SNP markers are utilized to perform molecular marker assisted breeding, so that the breeding period can be greatly shortened, cotton fiber strength is enhanced, and the breeding efficiency is improved.

The invention belongs to the technical field of rapeseed breeding, in particular to a method for breeding the self-incompatible two-line hybrid seeds of a brassica napus. The invention is characterized in that: aiming at the problem of being incapable of popularizing the self-incompatible hybrid seeds of the brassica napus in large area on production caused by the defects of the existing breeding method for the self-incompatible hybrid seeds of the brassica napus, the invention proposes a breeding method for the breeding the self-incompatible two-line hybrid seeds of the brassica napus, which comprises the breeding and propagating of the self-incompatible hybrid seeds of the brassica napus; and the breeding and propagating of the self-incompatible restorer line of the brassica napus as well as the breeding of the breeding the self-incompatible two-line hybrid seeds of the brassica napus and a production method for the seeds thereof. The invention provides an effective method for the breeding the self-incompatible hybrid seeds of the brassica napus, is favorable to the large area popularization of the self-incompatible hybrid seeds of the brassica napus and promotes the utilization on the advantages of hybrid seeds of the brassica napus.

The invention discloses a mandarin fish advanced artificial propagation method. The method comprises (1) selecting parent fish, (2) breeding parent fish, (3) artificial uteracon, (4) hatching, (5) breeding scrod, (6) breeding mating baitfish. Artificial breeding the parent fish in warmhouse originally, making the gonadal advance mature and reducing the reproductive cycle of the mandarin fish and getting the goal of breeding and sales in a same year and processing important social benefit and economic benefit using the technology of adjusting and controlling water temperature. During the breeding of mandarin scrod fish, early maturing breeding the soft-shelled turtle parent fish and advanced done artificial propagation to solve the problem of mating shedding bait fish for the scrod of the mandarin fish; providing mandarin fish with sufficient bait fish using the natural breeding of thecrucian in normal temperature condition.

The invention discloses a method of shortening the cultural period of the Takifugu flavidus, which includes the following steps: (1) selecting the Takifugu flavidus and the Takifugu rubripes at age between 3 and 5 in proportion of 2 to 1 in every March and April for parent fish induced mature culturing; (2) induced breeding and artificial egg collecting by using Luteinizing Releasing Hormone Analog (LRH-A); (3) artificial inseminating the Takifugu flavidus ovum with the Takifugu rubripes sperm; (4) fish fry culturing; (5) culturing in the outdoor earth pond when the body length of the fish fry is more than 3 cm; (6) culturing the fish fries in the fish fry room through the winter; (7) moving the overwintering fish to the outdoor fishpond when the outdoor water temperature is at more than 12 DEG C and going on the market after about five months' culture. The experimental culture result of the method shows that the average weight of the fish reaches more than 0.35 kg, wherein the maximum weight is 0.7 kg; the cultural period is shortened more than one time. The method reduces the culture cost by a big margin and greatly increases the Takifugu flavidus fish product culture benefit.

The invention relates to a method of increasing ginseng germ plasm saponin content, belonging to the biotechnology field. The steps of the method are as follows: 1. the plant expression vector pBI-ACAS of reversal cycloartenol synthetase CAS gene is constructed; 2. agrobacterium rhizogene A4-ACAS with an objective vector is obtained by the conversion of a bacterial strain of the agrobacterium rhizogene A4 by pBI-ACAS; 3. a ginseng hairy root clone for expressing the reversal CAS gene is produced by mediating a ginseng root explant with the agrobacterium rhizogene A4; a test-tube plant is obtained through the body cell embryogenesis with the ginseng hairy root clone that has high ginseng saponin content and expresses the reversal CAS gene as a culture material. In the invention, the ginseng hairy root clone that has high ginseng saponin content and new genetic resources of ginseng test-tube plants are constructed, different applications can be realized; through large-scale liquid culture of the hairy root clone, high-yield ginseng saponin can be obtained directly. If a new ginseng line with high ginseng saponin content is cultured by regeneration plant, the new ginseng line can be used in the cultivate production, and can obviously shorten the breeding cycle of a ginseng.

The invention discloses a breeding method for importing false smut resistance of indica rice into japonica rice. The method includes the following steps: (1) selecting japonica rice varieties/series to be improved and false smut resistant resources of the indica rice; (2) identifying the crude toxin tolerance concentration of the selected japonica rice varieties/series to be improved; (3) cross-breeding to obtain three-way cross coeno-species F1; (4) culturing anther of the three-way cross coeno-species F1 in a culture medium for culturing rows of indica rice; (5) field-planting flower cultured seedlings: planting a single anther cultured seedling to obtain a plurality of japonica rice flower cultured families with the false smut resistance improved, and further planting different flower cultured families in the field and screening to obtain new japonica rice varieties with good agronomic shape and improved false smut resistance, using the japonica rice varieties/series to be improved as a contrast. The breeding method provided by the invention can effectively import the false smut resistance of indica rice into japonica rice, and the false smut resistance breeding can be finished in about three years, therefore, the normal false smut resistance breeding period is greatly shortened; the breeding method has a great breeding application value.

The invention discloses a grafting propagation method for Lagerstroemia indica Pink Velour. The method includes the steps of 1, selecting grafting time; 2, growing rootstocks; 3, collecting scions; 4, combining the scions and the rootstocks; 5, performing post-grafting management. The method has the advantages such that excellent ornamental characteristic of Lagerstroemia indica Pink Velour can be retained, seedling raising cycle is shortened for Lagerstroemia indica Pink Velour, the method is simple to operate, healing is fast after grafting, and survival rate is high.

The invention provides a technology for cultivating seedless momordica grosvenori by combining plant tissue culture, polyploid induction and genetic breeding hybridization technologies, and belongs to the field of a modern biological technology. The momordica grosvenori obtained by the technology is great in fruit shape, high in mogroside content and high in whole fruit utilization rate, so that the current situation of a momordica grosvenori planting industry is completely changed, and the difficulty that the cost of mogroside is high and the mogroside is difficult to widely apply can be solved; and the application of the momordica grosvenori in industries of foods, beverages, medicines and the like is promoted and the development of a momordica grosvenori industry is accelerated.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY2 and a preparation method thereof. A nucleotide sequence of the gene is shown as SEQ ID NO: 1, and a protein with an aminoacid sequence shown as shown in SEQ ID NO: 2; according to the invention, functional genomics related technical researches prove that the LrWRKY2 gene has the function of improving the antifungal performance of plants; the antifungal LrWRKY2 gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for overexpression; the transgenic tobacco plantshave very strong fungal infection resistance, and experimental results show that the transgenic tobacco with over-expression of LrWRKY2 has high-level resistance to infection of alternaria oryzae, fusarium solani, fusarium verticillatum, Botryosphaeria dothidea and Alternaria panax.

The invention discloses a pinellia ternata in vitro tuber planting method and belongs to the agricultural biotechnological field. The method comprises the steps of (1) acquisition of in vitro tubers, (2) cleaning and processing, (3) soaking, (4) selection and processing of a matrix in a germination accelerating and rooting culture medium, (5) planting, (6) germination accelerating and rooting culture, and (7) field transplanting. The method has the advantages that the breeding cycle is shortened, operability is high, energy is saved, pest and disease damage can be effectively prevented, investment cost is low, management is easy, season limitation is avoided, and cultivation of the in vitro tubers can be conducted at any seasons suitable for growth based on the fact that the pinellia ternata in vitro tubers can be grown all year around. Germination accelerating and seedling culture of the in vitro tubers are key techniques for connecting tissue culture with field planting, large-scale artificial cultivation of pinellia ternate is guaranteed in the true sense, and the production application value is high. With pinellia ternate serving as a model plant, the promotion of the technique will lay a foundation for genetic resource conservation and artificial cultivation of other precious plants.

The invention discloses a molecular marker for a bacterial stripe resisting major gene BLS1 locus of rice and an application of the molecular marker. Specific steps for screening are as follows: (1) constructing a positioned segregation population, so as to obtain an approximate isogenic line F2 of the positioned segregation population; (2) extracting genomic DNA of rice leaves of each single strain of parents and a F2 population by adopting a CTAB method so as to carry out SSR molecular marker analysis; (3) preliminarily positioning a gene BLS1 in a region between RM19382 and RM510; (4) carrying out BLS1 close-linkage marking: carrying out detection and analysis after carrying out molecular marking by several kinds of marking primers, so as to position the BLS1 in a physical range, i.e., 21-kb between RM19400 and RM510. The molecular marker is applied to the selective breeding of bacterial stripe resisting rice varieties or the screening of resistant genetic resources. The molecular marker disclosed by the invention can be used for effectively detecting whether ordinary bacterial stripe resisting wild rice DP3 and derived varieties (lines) thereof contain the major gene locus or not, the efficiency of selection of bacterial stripe resisting rice is increased, and the bacterial stripe resisting rice varieties containing the gene BLS1 are obtained.

The invention discloses a method used for shortening nursery stage of taxus mairei seeds. According to the method, taxus mairei seeds at a certain maturation stage are collected; accelerating germination is realized by subjecting the collected seeds to seed coat artificial delaminating, variable temperature laminated storage and hormone treatment; and when 25% of the collected seeds are sprouted, the collected seeds are sown. Normal germination of the collected taxus mairei seeds can be realized in a next spring, germination of the seeds using the method is one year earlier than that using existing taxus mairei seed germination technology, nursery stage is shortened from 18 months to 6 months, and time period needed by germination of taxus mairei seeds is shortened greatly.

The invention discloses a watermelon seed seedling cultivation method capable of increasing the germination rate, and relates to the technical field of watermelon seedling growing. The method comprises the following steps of 1, pretreatment of seeds, 2, soaking of the seeds and germination; 3, preparation of seedling growing mediums; 4, sowing and seedling growing; 5, management of a seedling stage. According to the watermelon seed seedling cultivation method capable of increasing the germination rate, the seedling growing period is shortened, the seed germination rate and the seedling survival rate are increased, the index of strong seedlings is high, the root systems are developed, and melon vines are lush.

The invention discloses an industrialized seedling culture method for Chinese onions. A seedling shed is set up according to a conventional method, a seedbed is arranged in the seedling shed, soil in the seedbed is leveled, and ridges are formed. The method specifically includes the following steps of firstly, laying gauze on the seedbed; secondly, filling a nursery seedling plate with a matrix; thirdly, pre-processing seeds before sowing; fourthly, sowing the seeds; fifthly, conducting film mulching; sixthly, managing the seedbed; seventhly, conducting disease control at the seedling stage; eighthly, improving the growth of roots and seedlings. When Chinese onion seedling culture is conducted through the seedling culture technology, the number of seeds which are used is small, seedling emergence is quick, seedlings are robust and resist diseases, root systems are developed, the environment can be artificially controlled for industrialized seedling culture, mechanical transplantation is facilitated, seedling recovery is quick after transplantation, seedling lands are saved, the seedling culture cost is greatly saved, and good development prospects are achieved.

The invention discloses a technology which is not restricted by the climate factor of winter and capable of rapidly cultivating cotton seedlings by utilizing an idle curing barn in a seedling cultivation curing workshop, and using LED (light-emitting diode) lamps and fluorescent lamps with different wavelengths and matching ratios as light sources, aiming at the climate obstacles of low temperature and sparse sunlight confronted by seedling cultivation in winter in Hunan. With the adoption of the method, a seedling cultivation period can be greatly shortened to 35 days from 120 days, thus greatly reducing labour needs, and enabling the seedlings to be adaptive to a transplanted soil environment soon.

The invention discloses a fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels. According to the method, on the basis of the characteristic that buds sprout easily from axillary buds of the Elaeagnus mollis Diels, seedlings are obtained by germinating mature Elaeagnus mollis Diels seeds aseptically, tender stem segments with axillary buds or top buds are cut to serve as explants, germination and growth of the axillary buds are promoted with a tissue culture method, healthy and strong shoots with certain degree of lignification are obtained, the purpose of multiplication is realized, and then plant regeneration is realized through rooting culture. With adoption of the fixed bud multiplication method, the problem of high probability of browning occurring in callus and adventitious bud induction processes of a traditional Elaeagnus mollis Diels tissue culture method is solved, and the method is simple to operate and feasible.

The invention relates to a method for obtaining crowtoe regeneration plantlet by anther culture and belongs to the technical field of forage breeding. The method comprises the following steps: (A) obtaining flower buds at the late uninucleate stage, taking the flower buds by an ice box to a lab and refrigerating the flower buds at 4 DEG C for 2-3 days; (B) sterilizing the flower buds by sodium hypochlorite, using an integrated type microscope to isolate the flower buds and placing the isolated substances on a callus induction culture medium for culturing; (C) performing dark culture for 20 days, subculturing for 3-4 times every 15 days, placing the subcultured callus on an adventitious bud culture medium to culture, and inducing the callus to generate a bud; (D) performing rejuvenation culture to the bud, placing the bud on a root medium to culture for 15-20 days, and inducing roots; and (E) hardening the seedling, and transplanting the hardened seedling to obtain the regeneration plantlet. In the operations, steps (B) to (D) are performed under aseptic conditions; the dark culture in the step (C) is performed in a biochemical incubator; and the generation of the adventitious bud in the step (C) and the roots in the step (D) is performed in a manual climatic box.

The invention discloses a nutrition cup seedling raising method for brucea fruits, and belongs to the technical field of Chinese medicinal herb cultivation. The nutrition cup seedling raising method for the brucea fruits concretely comprises nine steps of selecting a seedling raising field, preparing nutrition cups, preparing nutrient soil, sowing, uncovering a mulch, shading, weeding, fertilizing and performing water management; the problems that requirements on land and climate are very strict when artificial cultivation of the Chinese herbal medicine brucea fruits is carried out, the transplant survival percent is low and seedlings grow slowly after being transplanted are effectively solved. The nutrition cup seedling raising method has the beneficial effects that seeds are saved, and are saved by 30 to 50 percent in comparison with bare-rooted sowing and seedling raising in field; the seedling raising period is shortened, and seedlings can grow up only by 4 to 5 months; as the seedlings carry original soil balls, and roots cannot be damaged, the transplant survival rate is high, and can reach more than 95 percent; moreover, after being transplanted, the seedlings grow fast, and the fruit yield of the brucea fruits is increased by more than 4 times.

The invention discloses a molecular marker which is closely linked with corn bacterial wilt resistance genes. The molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 in a sequence table or a nucleotide sequence as shown in SEQ ID NO.2 in the sequence table. A primer pair for amplifying the molecular marker is a nucleotide sequence as shown in SEQ ID NO.3 in the sequence table and a nucleotide sequence as shown in SEQ ID NO.4 in the sequence table. When the molecular marker is applied to corn bacterial wilt detection, amplification is performed through PCR, if an obtained PCR amplification product is a 234bp basic group fragment, the corn to be detected is of a bacterial wilt resistant variety, and if the obtained PCR amplification product is a 274bp alkali group fragment, the corn to be detected is of a bacterial wilt infected variety. By adopting the method, whether the corn has the bacterial wilt resistance or not can be detected before the corn is seeded, selective seeding can be achieved, the breeding time is shortened, and the method is rapid, simple and convenient and can be widely applied to assisted breeding of corn.

The invention relates to a method for culturing cephalataxus fortune. The method comprises the following steps of: a, furrowing and flattening the field, preparing seed bed fields, of which the width is 1.6m and the length is 1m, and paving the yellow soil which is 15 to 20cm in thickness on the surface of the field, wherein the effective area of each field is 12m<2>; b, collecting wild cephalataxus fortune branches, cutting the branches to 10 to 15cm, and binding each 50 cut branches into a bundle; c, soaking 2 to 3-centimeter base parts of the branches into a rooting agent, simultaneously placing the branches to be processed in an electric field generator, powering on the generator, and adjusting the voltage to 60 to 80 kilovolts, wherein the processing time is 30 minutes; d, planting the processed branches on the seed bed by cottage; and e, covering the seed bed by a film and a sun shading net. Compared with the cephalataxus fortune seedlings not processed by the method, the cephalataxus fortune seedlings cultured by the method have 3 to 5 more root hairs, the cottage survival rate is improved by 15 percent, and the seedling-raising period is shortened by 1 to 2 years; and the method for culturing the cephalataxus fortune contributes a lot to the development of the cephalataxus fortune industry.

The invention discloses a method for improving the growth trait of Fugu bimaculatus, comprising the following steps: (1) selecting female Fugu bimaculatus and male Fugu rubripes at the age of 3 to 5 to carry out parent fish mature-promoting culture annually from Match to April, wherein the quantity ratio is 2:1; (2) using luteotropin releasing hormone d-ala analog (LRH-A) to induce spawn; (3) carrying out artificial insemination on Fugu bimaculatus by semina of the Fugu rubripes; (4) culturing offspring seed; and (5) putting fish fry into pond for culturing when the total length of the fish fry is above 3cm. Adopting the method of the invention, the experimental culturing result shows that in northern areas of China, the average weight of the Fugu bimaculatus cultured for 16 months can reach more than 0.35kg, wherein the weight of a maximum individual is 0.45kg, and the culture cycle is shortened by more than one time, thereby greatly reducing culture cost and increasing the culture benefit of Fugu bimaculatus commercial fish.

The invention discloses an extraction method of gracilaria blodgettii protoplast. The technical scheme mainly comprises researching on the effects of different tool enzymes decomposing alga tissue, establishing the preparation method for gracilaria blodgettii protoplast, and obtaining of dissociated somatic cell protoplast. The advantages comprise that gracilaria blodgettii protoplast is successfully obtained, and is applicable to fields of genetic transformation, somatic cell hybridization, regeneration plant induction, somatic cell engineering seedling growing, etc.

The invention relates to a molecular marker of a rice seasonal febrile disease resistance gene Pigm and application of the molecular marker. The method for assisted-selection of seasonal febrile disease resistance rice by applying a molecular marker gm-4 comprises the following steps of: carrying out PCR (polymerase chain reaction) amplification by using DNA (deoxyribonucleic acid) of a rice genome as a template and gm-4 as a primer; and detecting an amplification product by using 2% agarose gel electrophoresis, if a 516bp stripe exists, a sample tested contains rice seasonal febrile disease resistance gene Pigm. The molecular marker has the beneficial effects that the genetic exchange does not exist through sequence difference design; and the molecular marker has high accuracy, is directly used for agarose gel electrophoresis detection, is relatively simple and convenient, is a co-dominant marker, can be used for detecting homozygous individuals and heterozygous individuals, shortens a breeding cycle and improves the efficiency.

The invention belongs to the technical field of plant molecular genetics, and specifically relates to a molecular marker for identifying early blossoming/late blossoming of lichee in the early stage and applications thereof. The molecular marker is characterized by comprising ZW1 and ZW2. The molecular marker ZW1 is prepared by amplifying an upstream primer represented by Seq ID No.1 and a downstream primer represented by Seq ID No.2. The molecular marker ZW2 is prepared by amplifying an upstream primer represented by Seq ID No.3 and a downstream primer represented by Seq ID No.4. The provided molecular marker and identification method have the advantages that the screening is rapid and accurate, the identification can be carried out in the early stage of seedlings, the identification method has an important meaning for breeding lichee species with the characteristics of early blossom and late blossom, the breeding period is prominently shortened, labor, materials, and land are largely saved, high economic and social benefits are generated, and the identification method has a wide application prospect.

The invention discloses a group of rapid marsdenia tenacissima propagation culture media. The main formula of the group of culture media is as follows: an induction medium of MS+6-BA 2.0mg/L+NAA 0.5mg/L; an enrichment medium of MS+6-BA 1.0mg/L+NAA 0.3mg/L; a sound seedling culture medium of MS+6-BA 1.0mg/L+NAA 0.3mg/L+NH4NO3 0.66g/L+NaH2PO4 0.56g/L+K2SO4 1.78g/L+banana 100g/L; and a rooting medium of 1/2MS+6-BA 0.5mg/L+IAA 0.2mg/L. The induction rate is over 88 percent, the proliferation rate is high, the rooting rate is over 95 percent, the tissue culture propagation period is short and is only 64-72 days, and effective and rapid vegetative propagation of the marsdenia tenacissima is realized.

The invention discloses a pair of special primers for assisted evaluation of soybean seed weight and a method thereof. The invention provides a method for assisted screening of soybean with seed weight property, comprising the following steps: the gene group DNA of soybean to be tested serves as a template, a Satt126 primer pair is used for carrying out PCR amplification, and an amplification product is tested; the soybean to be tested, which contains a DNA fragment between 165-175bp in the amplification product, is a candidate soybean with high seed weight property; the Satt126 primer pair is composed of ribotide represented by a sequence 1 and a sequence 2 in a sequence table; the high seed weight property is the property that kernel seed weight is 25.0 or more than 25.0 grams. The method of the invention overcomes the problem that breeding efficiency is low and the like because phenotypic selection is easy to be disturbed by environmental factors in normal breeding; the method of the invention can use the special primer marked by the Satt126 to carry out early generation choosing evaluation for the relevant locus of high seed weight of soybean so as to shorten breeding cycle, and then, high-yield soybean materials with bigger kernels can be quickly screened. The invention can be used for soybean high-yield breeding for improving breeding efficiency and soybean yield level.

The invention belongs to the field of plant breeding and in particular relates to a method for rapidly breeding early blooming and fruiting stock of apples. The method comprises the following steps: (1) acquiring crossbreeding descendants; (2) primarily selecting early blooming and fruiting stock of apples; (3) performing local experiments on the early blooming and fruiting stock of apples. By adopting the method, the breeding cycle can be greatly shortened by 3-5 years.

The invention relates to the technical field of seedling breeding, and particularly relates to a seedling breeding method of passiflora edulis. The method comprises the following steps: (1) base bed treatment, (2) seed pretreatment, (3) germination accelerating treatment, (4) sowing process, and (5) water and fertilizer management. According to the invention, a sowing and seedling breeding methodis adopted, so that the growth and differentiation of seed coat cells can be effectively promoted, the seedling breeding period is shortened, the stress resistance and the adaptability of the passiflora edulis to the environment are improved, and the survival rate is improved. With the adoption of the cultivation method provided by the invention, the survival rate of the passiflora edulis seedlings reaches 96% or above, and the incidence rate of the passiflora edulis seedlings is effectively decreased to 1.6% or below.

The invention discloses an anti-season fish propagation method comprising the steps such as construction of a culture pond, magnetic field treatment of parent fishes, cold and hot varied treatment ofthe parent fishes and strengthening treatment of an F1 generation. By using the method disclosed by the invention, the breeding period can be effectively shortened, the high and low temperature resistance of fishes can be improved, guarantees are provided for anti-season fish culture, the difficulty of anti-season culture of common fishes is lowered, the survival rate is increased by 88% or above,and the economic benefits of farmers are increased.

The invention discloses a seed container seedling raising method of rare woody oil crop Mongolia almonds. The method comprises the steps of seed collection, seed selection, after-ripening treatment onseeds, stratification, endocarp removal and the like. By means of the method, the emergence speed is high, the emergence rate is high, the seeds are saved, the method is simple and convenient, materials are obtained locally, the quality of cultured seedlings is good, the seedling raising cost is low, the field planting survival rate is high, and the method is a good method for saving and expanding propagation of Mongolian almonds.