100results about How to "Shorten the breeding cycle" patented technology

Breeding method for importing false smut resistance of indica rice into japonica rice

ActiveCN104542256AEfficient importShorten the breeding cyclePlant tissue cultureHorticulture methodsCross breedingJaponica rice
The invention discloses a breeding method for importing false smut resistance of indica rice into japonica rice. The method includes the following steps: (1) selecting japonica rice varieties/series to be improved and false smut resistant resources of the indica rice; (2) identifying the crude toxin tolerance concentration of the selected japonica rice varieties/series to be improved; (3) cross-breeding to obtain three-way cross coeno-species F1; (4) culturing anther of the three-way cross coeno-species F1 in a culture medium for culturing rows of indica rice; (5) field-planting flower cultured seedlings: planting a single anther cultured seedling to obtain a plurality of japonica rice flower cultured families with the false smut resistance improved, and further planting different flower cultured families in the field and screening to obtain new japonica rice varieties with good agronomic shape and improved false smut resistance, using the japonica rice varieties/series to be improved as a contrast. The breeding method provided by the invention can effectively import the false smut resistance of indica rice into japonica rice, and the false smut resistance breeding can be finished in about three years, therefore, the normal false smut resistance breeding period is greatly shortened; the breeding method has a great breeding application value.

Pinellia ternata in vitro tuber planting method

The invention discloses a pinellia ternata in vitro tuber planting method and belongs to the agricultural biotechnological field. The method comprises the steps of (1) acquisition of in vitro tubers, (2) cleaning and processing, (3) soaking, (4) selection and processing of a matrix in a germination accelerating and rooting culture medium, (5) planting, (6) germination accelerating and rooting culture, and (7) field transplanting. The method has the advantages that the breeding cycle is shortened, operability is high, energy is saved, pest and disease damage can be effectively prevented, investment cost is low, management is easy, season limitation is avoided, and cultivation of the in vitro tubers can be conducted at any seasons suitable for growth based on the fact that the pinellia ternata in vitro tubers can be grown all year around. Germination accelerating and seedling culture of the in vitro tubers are key techniques for connecting tissue culture with field planting, large-scale artificial cultivation of pinellia ternate is guaranteed in the true sense, and the production application value is high. With pinellia ternate serving as a model plant, the promotion of the technique will lay a foundation for genetic resource conservation and artificial cultivation of other precious plants.

Molecular marker for bacterial stripe resisting major gene BLS1 locus of rice and application of molecular marker

The invention discloses a molecular marker for a bacterial stripe resisting major gene BLS1 locus of rice and an application of the molecular marker. Specific steps for screening are as follows: (1) constructing a positioned segregation population, so as to obtain an approximate isogenic line F2 of the positioned segregation population; (2) extracting genomic DNA of rice leaves of each single strain of parents and a F2 population by adopting a CTAB method so as to carry out SSR molecular marker analysis; (3) preliminarily positioning a gene BLS1 in a region between RM19382 and RM510; (4) carrying out BLS1 close-linkage marking: carrying out detection and analysis after carrying out molecular marking by several kinds of marking primers, so as to position the BLS1 in a physical range, i.e., 21-kb between RM19400 and RM510. The molecular marker is applied to the selective breeding of bacterial stripe resisting rice varieties or the screening of resistant genetic resources. The molecular marker disclosed by the invention can be used for effectively detecting whether ordinary bacterial stripe resisting wild rice DP3 and derived varieties (lines) thereof contain the major gene locus or not, the efficiency of selection of bacterial stripe resisting rice is increased, and the bacterial stripe resisting rice varieties containing the gene BLS1 are obtained.

Method for obtaining crowtoe regeneration plantlet by anther culture

InactiveCN103004599AShorten the breeding cycleGood gene functionPlant tissue cultureHorticulture methodsCell buddingCallus formation
The invention relates to a method for obtaining crowtoe regeneration plantlet by anther culture and belongs to the technical field of forage breeding. The method comprises the following steps: (A) obtaining flower buds at the late uninucleate stage, taking the flower buds by an ice box to a lab and refrigerating the flower buds at 4 DEG C for 2-3 days; (B) sterilizing the flower buds by sodium hypochlorite, using an integrated type microscope to isolate the flower buds and placing the isolated substances on a callus induction culture medium for culturing; (C) performing dark culture for 20 days, subculturing for 3-4 times every 15 days, placing the subcultured callus on an adventitious bud culture medium to culture, and inducing the callus to generate a bud; (D) performing rejuvenation culture to the bud, placing the bud on a root medium to culture for 15-20 days, and inducing roots; and (E) hardening the seedling, and transplanting the hardened seedling to obtain the regeneration plantlet. In the operations, steps (B) to (D) are performed under aseptic conditions; the dark culture in the step (C) is performed in a biochemical incubator; and the generation of the adventitious bud in the step (C) and the roots in the step (D) is performed in a manual climatic box.

Method for culturing cephalataxus fortunei

InactiveCN102197775AImprove the survival rate of cuttingsShorten the breeding cycleSaving energy measuresCultivating equipmentsElectric fieldRoot hair
The invention relates to a method for culturing cephalataxus fortune. The method comprises the following steps of: a, furrowing and flattening the field, preparing seed bed fields, of which the width is 1.6m and the length is 1m, and paving the yellow soil which is 15 to 20cm in thickness on the surface of the field, wherein the effective area of each field is 12m<2>; b, collecting wild cephalataxus fortune branches, cutting the branches to 10 to 15cm, and binding each 50 cut branches into a bundle; c, soaking 2 to 3-centimeter base parts of the branches into a rooting agent, simultaneously placing the branches to be processed in an electric field generator, powering on the generator, and adjusting the voltage to 60 to 80 kilovolts, wherein the processing time is 30 minutes; d, planting the processed branches on the seed bed by cottage; and e, covering the seed bed by a film and a sun shading net. Compared with the cephalataxus fortune seedlings not processed by the method, the cephalataxus fortune seedlings cultured by the method have 3 to 5 more root hairs, the cottage survival rate is improved by 15 percent, and the seedling-raising period is shortened by 1 to 2 years; and the method for culturing the cephalataxus fortune contributes a lot to the development of the cephalataxus fortune industry.

A pair of special primers for assisted evaluation of relevant locus of soybean seed weight and method thereof

InactiveCN101613753AShorten the breeding cycleImprove breeding efficiency and soybean yield levelsMicrobiological testing/measurementPlant genotype modificationGramAnimal science
The invention discloses a pair of special primers for assisted evaluation of soybean seed weight and a method thereof. The invention provides a method for assisted screening of soybean with seed weight property, comprising the following steps: the gene group DNA of soybean to be tested serves as a template, a Satt126 primer pair is used for carrying out PCR amplification, and an amplification product is tested; the soybean to be tested, which contains a DNA fragment between 165-175bp in the amplification product, is a candidate soybean with high seed weight property; the Satt126 primer pair is composed of ribotide represented by a sequence 1 and a sequence 2 in a sequence table; the high seed weight property is the property that kernel seed weight is 25.0 or more than 25.0 grams. The method of the invention overcomes the problem that breeding efficiency is low and the like because phenotypic selection is easy to be disturbed by environmental factors in normal breeding; the method of the invention can use the special primer marked by the Satt126 to carry out early generation choosing evaluation for the relevant locus of high seed weight of soybean so as to shorten breeding cycle, and then, high-yield soybean materials with bigger kernels can be quickly screened. The invention can be used for soybean high-yield breeding for improving breeding efficiency and soybean yield level.
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