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SNP molecular marker associated with chromosome 6 and fiber strength of upland cotton

A technology of molecular markers and fiber strength, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of less cotton application, complex genetic background, lack of reliability and stability, etc., and achieve improvement Effects of breeding efficiency, improving fiber quality and shortening breeding cycle

Active Publication Date: 2017-06-20
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0005] In conclusion, SNP markers are currently the molecular markers with the most potential for development, and have been widely used, but they are still less used in cotton. 2 、BC 1 , the genetic background is complex, or the results obtained only in a single environment, so the reliability and stability are lacking, and there are few stable QTLs in multiple environments, and the initial purpose of some studies is only for the positioning of target genes

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  • SNP molecular marker associated with chromosome 6 and fiber strength of upland cotton
  • SNP molecular marker associated with chromosome 6 and fiber strength of upland cotton

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Embodiment Construction

[0021] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0022] (1) Recombinant inbred line F 6:8 the acquisition

[0023] For the field planting and DNA extraction from 2007 to 2008, please refer to the patent application publication number: CN 101613761A, the title of the invention: the patent application document of the SSR marker linked to the main effect gene of cotton fiber strength. In 2009, the parents and F 6:10 group. Anyang and Quzhou adopt a single-row area with a length of 5 meters and a row spacing of 0.8m and (0.8+0.5)m respectively, with 20 plants in each row; Xinjiang adopts a 6-row area with a length of 2 meters and 15 plants in each row. In 2010, the parents and F 6:11 For groups, Anyang and Zhengzhou adopt single-track areas with a row length of 5m and a row spacing of 0.8m; Gaoyi adopts a single-row area with...

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Abstract

The invention belongs to the technical field of molecular breeding of cotton and discloses an SNP molecular marker associated with fiber strength of upland cotton as well as detection and application thereof. The SNP molecular marker is obtained by taking stable RIL group of cotton as the material through a genome resequencing method. When the SNP markers are used for carrying out molecular marker-assisted breeding selection, the breeding period can be greatly shortened; the breeding efficiency of the cotton can be improved; the cotton fiber strength can be improved.

Description

technical field [0001] The invention belongs to the technical field of cotton molecular breeding, and in particular relates to a SNP molecular marker related to the fiber strength of upland cotton and its detection and application. Background technique [0002] As a major fiber crop, cotton plays an important role in the world economy. Among the four cotton species widely cultivated in the world, upland cotton occupies the most important position, and its output accounts for more than 90% of the world's total cotton output. With the increase of people's demand for medium and high-grade textiles, the requirements for fiber quality are increasing day by day, but the traditional breeding methods are mainly based on phenotype selection, the breeding efficiency is low, and it is difficult to meet the needs of quality breeding. The development of molecular marker technology has made it possible to directly select the genotypes of quantitative traits. QTL mapping by constructing ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 商海红巩万奎范森淼张震袁有禄石玉真霍鹏葛群刘爱英龚举武李俊文
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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