123 results about "Genome resequencing" patented technology

The present invention is an improved method of resequencing DNA using microarrays to rapidly map and identify SNPs, deletions and amplification events present in the genome of an organism. The method is performed by hybridizing a reference and a test genome to two separate arrays with each array exhibiting a specific intensity pattern. The intensity differences between the reference and the test genome arrays are used to produce a mutation map. The mapped differences are resequenced on a set of resequencing arrays to identify specific genetic mutations.

The invention discloses a method for rapidly identifying a transgenic or gene editing material and an insertion site thereof by using whole genome resequencing data. The method comprises the followingsteps: extracting a genome DNA; obtaining the double-end sequencing data of the whole genome; judging whether an expression vector sequence inserted into the plant to be detected and containing the T-DNA sequence is known or not; judging whether the to-be-detected plant has a transgenic event or a gene editing event or not, and whether a skeleton sequence transfer event occurs or not; and determining an insertion site of the T-DNA sequence. According to the invention, the method combines with a bioinformatics analysis means; under the condition that an expression vector is known or unknown, whether a transgenosis or gene editing event occurs or not is identified; under the condition that an expression vector is known, the accurate positioning, direction, copy number and flanking sequenceinformation of a target sequence inserted into a genome can be rapidly and accurately given; whether a skeleton sequence, namely a sequence except the target sequence, is inserted into the genome or not can also be identified, and the same positioning is given.

The present invention provides novel method for increasing the efficiency and accuracy of high-throughput mutation mapping and genome resequencing by using a variable length probe selection algorithm to rationally select probes used in designing oligonucleotide arrays synthesized by Maskless Array Synthesis (MAS) technology. Also disclosed is a variable length probe selection algorithm used in designing such oligonucleotide arrays.