128 results about "Genome resequencing" patented technology

The invention relates to the technical field of molecular detection, and particularly discloses a liquid chip for identifying opisthopapus taihangensis and Hebei province as well as a preparation method and application of the liquid chip. Genotyping sites of the liquid chip comprise 1140 SNP sites; the physical positions of the 1140 SNP loci are determined based on comparison of a whole genome sequence of a bovine reference genome, and the version number of the whole genome sequence of the bovine reference genome is ARSUCD 1.2; wherein the physical position information of the 1140 SNP loci is as shown in a table 1. The liquid chip can realize genetic typing of the opisthopapus taihangensis and the Hebei province cattle, has the advantage of high detection rate, and can obviously cluster the opisthopapus taihangensis and the Hebei province cattle into two classes when clustering analysis is carried out on the opisthopapus taihangensis and the Hebei province cattle, so that a marker combination of the liquid chip can represent whole genome re-sequencing genes, assists in distinguishing the opisthopapus taihangensis and the Hebei province cattle, and is high in accuracy and high in accuracy. Therefore, the variety identification of the opisthopapus taihangensis and the Hebei Hebei is realized.

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The invention discloses a peanut quality character selective breeding method based on whole genome SNP and application, and relates to the technical field of crop breeding, the method comprises the specific steps of data acquisition, SNP optimization and marker screening, model construction and new strain breeding; by integrating whole genome re-sequencing, SNP optimization marker screening and mixed deep learning model construction, peanut quality character prediction and breeding value evaluation are achieved, through whole genome re-sequencing and quality control processes, an SNP variation map is obtained, ten key quality characters including protein, oil content, oleic acid and the like are covered, and the breeding value of peanuts is evaluated. According to the method, comprehensive genetic information is provided for subsequent analysis, a feature marker set is formed by screening out sites in the SNP optimization and marker screening link, interference of low-quality sites is avoided, a whole genome selection model is obtained by adjusting and training a mixed deep learning model and optimizing hyper-parameters through ten-fold cross validation, the breeding period is shortened, and the breeding efficiency is improved. And the prediction error rate is reduced.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention belongs to the technical field of aquatic product breeding, and particularly relates to a KASP molecular marker related to high temperature resistance character of patinopecten yessoensis and application of the KASP molecular marker in genetic breeding. On the basis of genome re-sequencing of 200 patinopecten yessoensis, a PySNP587919 locus obviously associated with the patinopecten yessoensis high-temperature-resistant character is screened by combining a whole genome SNP genotype and whole genome association analysis of the patinopecten yessoensis high-temperature-resistant character, and after the reliability of the locus is verified through Sanger sequencing, the locus is developed into a KASP marker, and the KASP marker can be used for identifying the high-temperature-resistant character of the patinopecten yessoensis and identifying the high-temperature-resistant character of the patinopecten yessoensis. The screening method can be used for screening the high-temperature-resistant comb shells. Furthermore, the invention also establishes a method for screening the high-temperature-resistant patinopecten yessoensis, and the method is efficient and accurate. Therefore, the technical scheme provided by the invention is suitable for molecular marker-assisted breeding work, rapid and effective screening of parents can be realized by typing the PySNP587919 site, and the breeding efficiency is improved.

The invention discloses a fig germplasm resource diversity evaluation system and a fig germplasm resource diversity evaluation method, and relates to the field of comprehensive evaluation.The fig germplasm resource diversity evaluation method comprises the steps that after multi-climate zone fig germplasm resources are collected and samples are processed in a standardized mode, 32 phenotypic characters are obtained in combination with hyperspectral imaging, 3D morphological scanning and a physiological sensor; a UPLC-QTOF-MS technology is utilized to construct metabolite fingerprints of leaves and fruits, and an SNP / InDel molecular marker is developed through whole genome re-sequencing for genotyping. Integrating stress resistance physiological data in a stress environment, and calculating a comprehensive score by adopting an analytic hierarchy process. And screening core evaluation indexes through weighted principal component analysis, constructing a similarity matrix in combination with an improved Shannon-Wiener index and a Neii's genetic distance algorithm, and finally forming a germplasm clustering map and a genetic diversity thermodynamic diagram. The method has the advantages that germplasm representativeness is guaranteed through multi-climate-zone sampling, multi-dimensional data are integrated, core indexes are accurately analyzed and screened, a visual atlas is finally generated, and scientific support is provided for germplasm research and breeding.

The invention discloses a Chinese rose petal outer edge notch whole genome SNP molecular marker combination and application thereof, and aims to overcome the defects in the prior art, 879 SNP markers significantly related to petal outer edge notch are identified by fusing 358 Chinese rose whole genome re-sequencing data and petal outer edge notch phenotype correlation analysis and applying a multi-model combined screening technology, and the SNP markers are used for identifying the petal outer edge notch. And a whole genome selection model with high prediction accuracy is established. The markers are distributed on 14 chromosomes, high-throughput and rapid detection can be achieved, the detection period is shortened, the petal wave character is directly predicted through seedling genotype data, and the breeding process is accelerated. The method has the advantages of low cost, high throughput and industrialization potential, provides key data support for Chinese rose petal outer edge notch genetic law analysis and molecular marker assisted breeding, and significantly improves the breeding efficiency and precision.

The invention belongs to the field of molecular breeding, and particularly discloses a molecular marker for identifying the width of tartary buckwheat seeds, a molecular marker primer group and application of the molecular marker, the SNP site of the molecular marker is located at the 22515278 bp position of the first chromosome of a tartary buckwheat reference genome, and the base polymorphism of the molecular marker is G / T; according to the invention, an F2 segregation population constructed by a wide-grain material KQ-1 and a narrow-grain material JQ-2 is subjected to whole genome re-sequencing and correlation analysis of grain width phenotype data, and an SNP locus which is extremely remarkably related to grain width is identified in a 22.51 Mbp interval of a first chromosome of tartary buckwheat; the genotype of the locus is highly consistent with the grain width phenotype, and the locus can be directly used for rapid identification of tartary buckwheat germplasm resources, early screening of filial generations and molecular marker-assisted breeding of high-yield tartary buckwheat varieties, and has important application value.

The invention relates to the technical field of intelligent agriculture and crop models, and particularly discloses a corn whole growth period simulation model construction method based on gene information. The method comprises the following steps: firstly, obtaining whole genome re-sequencing data of a target corn population and multi-growth-stage phenotype data of continuous years at the same ecological point, and dividing the population into a plurality of subgroups with consistent genetic backgrounds based on the re-sequencing data; and then, by adopting a strategy of'single family parameter inversion-subgroup template construction-secondary optimization and calibration on a single genotype under the constraint of the subgroup template ', the genetic subgroup characteristics based on the genome are associated with an APSIM crop model, and a high-precision corn growth period prediction model is constructed. According to the method, the defects that a traditional crop model is low in large-scale breeding material parameter calibration efficiency and prone to falling into local optimum are overcome, and the method is particularly suitable for efficient and accurate growth period prediction and adaptability evaluation on a large number of breeding materials in a unified ecological region.

The invention discloses a nuclear factor YB subunit gene GhNF-YB3 and application thereof. The nucleotide sequence of the gene GhNF-YB3 is shown as SEQ ID NO. 1, and the nucleotide sequence of the gene GhNF-YB3 is shown as SEQ ID NO. The site gene GhNF-YB3 significantly related to the cotton yield trait is obtained by performing yield trait investigation on 245 upland cotton varieties and combining population genome re-sequencing and ovule transcriptome sequencing one day after flowering to perform GWAS and eQTL positioning. Gene expression of the gene GhNF-YB3 is in significant positive correlation with two yield traits, namely seed index and boll weight, and the gene GhNF-YB3 may be a causal gene for regulating and controlling the traits of the seed index of cotton. The nuclear factor YB subunit gene GhNF-YB3 disclosed by the invention can be applied to identification of high-yield upland cotton varieties and improvement of cotton yield traits.

The application discloses a kind of silver carp variety specific SNP molecular marker combination, silver carp DNA fingerprint and application, the SNP molecular marker combination includes 367 SNP sites, the site information of the SNP molecular marker combination is as shown in table 1.The application takes silver carp germplasm resource as research object, obtains genotype by whole genome resequencing method, and then filters out 367 specific SNP markers capable of distinguishing different silver carp varieties, and the DNA fingerprint based on these markers can be used to distinguish different silver carp varieties.Compared with traditional methods, the fingerprint has the characteristics of high accuracy and strong reliability when used for silver carp variety identification.This technology not only provides strong support for the specific evaluation of silver carp varieties, the protection and management of breed rights, and the optimization and structure adjustment of silver carp breeding industry, but also shows good application prospects in the classification of silver carp germplasm resources, the confirmation of breed rights and the tracing of individual sources.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention provides a method for screening candidate genes and SNP (Single Nucleotide Polymorphism) loci related to residual feed intake of Sahu hybrid sheep, which is characterized by comprising the following steps: S1, collecting jugular vein blood samples of the Sahu hybrid sheep, and extracting genomic DNA (Deoxyribose Nucleic Acid) of the blood samples for quality detection; the method comprises the following steps: S1, extracting DNA, S2, carrying out whole genome re-sequencing on the extracted DNA and carrying out genotyping to obtain SNP genotype data, and S3, carrying out reference genome comparison, SNP detection and genotype quality control. And S4, carrying out whole genome association analysis on the residual feed intake character of the Sahu hybrid sheep to obtain a significant SNP site. The nucleotide sequence of the SNP site obviously related to the residual feed intake of the Sahu hybrid sheep, which is obtained by the method provided by the invention, is as shown in SEQ ID NO.1, the basic group R at the 51st site of the sequence is A or G, the gene mutation causes the nucleotide of the sequence to generate polymorphism, and when the marker is mutated into G, the Sahu hybrid sheep shows lower residual feed intake.

The invention relates to the technical field of plant molecular markers, in particular to a millet whole genome 65K liquid chip and application thereof.The millet whole genome 65K liquid chip is obtained through the following steps that re-sequencing data of a millet material is obtained, and then the re-sequencing data is subjected to quality control, mapping with a reference genome and identification and screening of variation sites; according to the indexes of the variation sites, combining the positions of the sites on the chromosomes, analyzing upstream and downstream sequences of the variation sites, and selecting the variation sites which can be used for chip development; the obtained variation site and 50bp sequences before and after the variation site are used, and an Infinium chip manufacturing technology is used for manufacturing the millet whole genome 65K liquid phase chip; based on high-depth whole genome re-sequencing data of large-scale millet germplasm materials, the millet whole genome 65K liquid chip is successfully developed, and can be widely applied to the fields of millet genotype detection, variety identification, genetic positioning, genetic diversity analysis, whole genome correlation analysis, whole genome selective breeding and the like. And important technical support is provided for molecular breeding development of millet.

The application discloses a SNP site associated with cotton boll node height character and application thereof. Based on whole genome resequencing data of a natural population of upland cotton and two years of field phenotypic investigation, a stable SNP site associated with cotton boll node height is identified through whole genome association analysis, the site is located at the base of 15876258bp on the A05 chromosome of upland cotton, the site is a single base mutation from T to C, and the boll node height of cotton of the TT type is significantly higher than that of cotton of the CC type. In the mechanical harvesting process of cotton, the boll node height is an important parameter for determining whether a cotton variety is suitable for mechanical harvesting. The SNP site associated with the cotton boll node height character provided by the application can be applied to screening of germplasm resource materials of upland cotton suitable for mechanical harvesting, and can also be applied to molecular marker assisted breeding of mechanical harvesting cotton materials, so as to accelerate the breeding process of new cotton varieties suitable for mechanical harvesting, and lay a foundation for cotton full-process mechanization.

This invention provides a method for identifying abamectin resistance genes in the fall armyworm, dsRNA, and their applications, belonging to the field of molecular biology. This invention combines genome resequencing and transcriptome sequencing to identify key abamectin resistance genes in the fall armyworm. Based on the conserved mRNA sequences of the key resistance genes GSTD6 and CTP6A18, dsRNA was designed, and RNAi technology can effectively reduce the fall armyworm's resistance to abamectin. This has significant practical implications for developing effective fall armyworm resistance management strategies to delay the emergence of resistance and for identifying new targets for pest control.

The application discloses a nuclear factor YB subunit gene GhNF-YB3 and application thereof, the nucleotide sequence of the gene GhNF-YB3 is shown as SEQ ID NO. 1; through yield character investigation on 245 upland cotton varieties, GWAS and eQTL positioning are carried out in combination with population genome resequencing and ovule transcriptome sequencing one day after flowering, and a site gene GhNF-YB3 significantly related to cotton yield character is obtained. The gene expression of the gene GhNF-YB3 is significantly positively correlated with two yield characters of seed index and boll weight, and the gene may be a causal gene for regulating cotton seed index character. The nuclear factor YB subunit gene GhNF-YB3 of the application can be applied to identification of high-yield upland cotton varieties and improvement of cotton yield character.

The invention discloses a detection method and application of a molecular marker related to cattle cold adaptability. On the basis of large-batch whole genome re-sequencing data of northern cattle and southern cattle, a genome selection signal analysis method is adopted, and multi-omics verification such as transcriptome and epime is combined, so that the molecular marker related to the cold environment adaptability is screened out. The molecular marker can be used for cold adaptive molecular breeding of cattle population, and provides scientific basis and technical support for genetic improvement and resource utilization of cattle species adaptive to cold environment.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

This invention discloses a combination of SNP molecular markers for sweet potato, an SNP chip, and their applications, relating to the fields of plant biotechnology and plant molecular breeding. The chip contains 16,730 SNP loci located on chromosome 15 of the sweet potato reference genome "Y22". This sweet potato 16K liquid-phase SNP chip, SweetpotatoGBTS16K, is applied to genotyping, variety identification, gene mapping, and genome-wide association analysis of sweet potato varieties. The SNP loci on the liquid-phase chip of this invention were screened from large-scale sweet potato genome resequencing data, totaling 16,730 SNP loci, encompassing associated loci for major agronomic traits such as yield, quality, and resistance in sweet potato, and has broad application prospects in multiple fields of sweet potato breeding.

The application discloses a gene prdR for regulating myxobacteria predation and a protein coded by the gene, and belongs to the technical field of biotechnology. The mutant DK10 with significantly improved predation capacity is obtained by using the strategy of adaptive evolution; genetic variation sites of the mutant DK10 are analyzed by using the bacterial genome resequencing technology, and functions of the genetic variation sites are researched by using the gene knockout technology, and it is found that the gene deletion of MXAN_2902 (prdR, the nucleotide sequence is shown as SEQ ID NO. 1) significantly enhances the predation capacity of Myxococcus xanthus. The nucleic acid sequence and the amino acid sequence of the transcriptional activator PrdR for regulating the predation of Myxococcus xanthus are disclosed, and the functional strain of Myxococcus xanthus capable of efficiently predating Salmonella enteritidis can be developed by using the prdR gene, and a new strategy is provided for the prevention and control of drug-resistant HVPG pathogenic bacteria.

The invention belongs to the field of biological detection, and relates to an SNP (Single Nucleotide Polymorphism) combination for identifying hybrid lineage of foreign varieties and northeast local beef cattle and an application method of the SNP combination. A whole genome re-sequencing technology is utilized, northeast local beef Yanbian cattle is taken as a target group, European common cattle is taken as a reference group, Asian common cattle is taken as a transition group, and variation sites and a group genetic structure of northeast local beef cattle are analyzed; it is found that hybrid individuals of lizan or simmental and Yanbian cattle of foreign varieties exist in local beef cattle groups in northeast China. By calculating inter-population differentiation index (Fst), linkage imbalance (LD) and allele frequency (AF) of variation sites in the population, 1640 SNP sites are screened, and the lineage of Yanbian cattle can be identified.

This invention discloses a pea genome-wide 20K SNP liquid-phase breeding chip and its application. This chip contains 21,659 SNP loci from the pea genome, specifically based on agronomic trait association analysis and whole-genome resequencing data from pea samples, along with resequencing data from various varieties. High-quality, representative SNP loci of 21,659 were selected from these data. This chip can be widely used for pea genotyping, contributing to improved pea breeding efficiency and pea variety innovation.

The present application relates to the field of molecular genetic technology, in particular to a KASP molecular marker TMSK23 related to eggplant reverse temperature-sensitive male sterility and application thereof. The molecular marker site is obtained by crossing 05ms as female parent and S132 as male parent, and obtaining F2 separation population. According to the published high-quality eggplant genome information, the whole genome resequencing technology is used to pool and sequence the homozygous dominant fertile plants and homozygous recessive sterile plants in the F2 generation of eggplant, and the SNP difference sites of the fertile gene and sterile gene pool are screened out, and the difference sites are verified by KASP gene typing method and protein three-dimensional structure prediction. The present application solves the problems of large workload in hybrid seed production in the prior art, avoids the problem of a large number of measurement and matching in the transgenic process, improves the breeding efficiency and has other advantages.

The invention relates to the technical field of crop molecular breeding and biology, and discloses a sorghum whole genome 24K-SNP liquid chip and application thereof.The chip obtains 24062 SNP loci through process screening on the basis of whole genome re-sequencing data of global sorghum germplasm, and screening standards include that variation with the minimum allele frequency larger than 0.01 is reserved; it is ensured that the site flanking 100bp sequence is uniquely compared in a reference genome, and the consistency reaches 100%; removing redundant variation within 30bp at the downstream of the site; and implementing a distribution strategy that 6-7 sites are reserved in each 100Kb interval, a coding region is anchored preferentially, and a centromere region is filled. According to the method, the specific probe is used for liquid-phase hybrid capture, so that the method has the advantages of wide genetic background coverage, high capture specificity, uniform genome coverage and the like, can effectively solve the genetic typing problem of wild species and different-place germplasm, and is widely applicable to sorghum germplasm resource identification, whole-genome association analysis and molecular breeding research.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

The application discloses a screening method and application of a SNP molecular marker of a PDGF gene related to the number of lambing of a Tianhua mutton sheep flock. F ST The signal is detected, and a molecular marker affecting the number of lambing of the Tianhua mutton sheep flock is screened in combination with gene annotation and site annotation. The molecular marker is located at 3850004 bp of a PDGFD gene of a chromosome 15 of a sheep reference genome (ARS-UI_Ramb_v2.0), and the base mutation is T or C. The molecular marker has a significant influence on the number of lambing of the Tianhua mutton sheep flock, can be applied to the molecular marker for detecting the number of lambing of the Tianhua mutton sheep flock, accelerates the breeding process of the Tianhua mutton sheep, and has the advantages of simple operation, high speed, high sensitivity and the like.

The invention discloses a chenopodium quinoa willd pre-harvest sprouting resistance molecular marker and a screening method and application thereof, and belongs to the technical field of biology, a high-resistance variety QQ65 and a susceptible variety Faro are utilized to construct an F2 segregation population, and through whole genome re-sequencing and map-based cloning, a major QTL for controlling the pre-harvest sprouting resistance of chenopodium quinoa willd is finely positioned in a physical interval of about 60 kb in a chromosome 8. In the interval, low expression of the gene LOC110693583 in the seed germination process of a resistant material is found, high expression of the gene LOC110693583 is induced in a susceptible material, and the sequence variation of the gene LOC110693583 is highly co-separated from the resistance phenotype of the gene LOC110693583. The unique resistance haplotype of the QQ65 in the region is further determined through re-sequencing analysis on a large number of germplasm resources.

The invention relates to the field of molecular breeding of Chinese pears, and discloses an SNP (Single Nucleotide Polymorphism) marker related to the fruit peel color of Chinese pears, the SNP marker is located at the No.8 chromosome 4163889 site of a 20-century pear PPYr1.0 genome, the fruit peel color of the Chinese pears is green when the genotype is T / T, and the fruit peel color of the Chinese pears is brown when the nucleotide at the SNP site is C / C or C / T. Compared with the prior art, the SNP molecular marker has the following advantages and effects that genome re-sequencing and whole genome association analysis (GWAS) are utilized to enrich in a gene interval related to the fruit peel color of the Chinese pears, the SNP molecular marker closely linked with the target character is developed, the SNP molecular marker can be effectively used for molecular marker-assisted selective breeding of the Chinese pears, the accuracy of character selection of the fruit peel color of the Chinese pears is improved, and the SNP molecular marker can be applied to the molecular marker-assisted selective breeding of the Chinese pears. The breeding process is accelerated.