123 results about "Genome resequencing" patented technology

The invention discloses a method for rapidly identifying a transgenic or gene editing material and an insertion site thereof by using whole genome resequencing data. The method comprises the followingsteps: extracting a genome DNA; obtaining the double-end sequencing data of the whole genome; judging whether an expression vector sequence inserted into the plant to be detected and containing the T-DNA sequence is known or not; judging whether the to-be-detected plant has a transgenic event or a gene editing event or not, and whether a skeleton sequence transfer event occurs or not; and determining an insertion site of the T-DNA sequence. According to the invention, the method combines with a bioinformatics analysis means; under the condition that an expression vector is known or unknown, whether a transgenosis or gene editing event occurs or not is identified; under the condition that an expression vector is known, the accurate positioning, direction, copy number and flanking sequenceinformation of a target sequence inserted into a genome can be rapidly and accurately given; whether a skeleton sequence, namely a sequence except the target sequence, is inserted into the genome or not can also be identified, and the same positioning is given.