13 results about "Brassica oleracea" patented technology

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

The invention discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and the sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 4 in a sequence table. By applying the primer group and the molecular marker, the fertility of the broccoli can be efficiently and accurately identified, the problem that 50% of fertile plants need to be pulled out in seed production is solved, and the manpower and material resource cost is reduced.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The invention discloses a method for high-yield cultivation of oxheart cabbages by using mountain tea garden conditions, relates to the technical field of cabbage planting, and aims to solve the problems of low resource utilization rate of northern-Sichuan mountain special tea gardens, poor cultivation suitability of spring-stubble oxheart cabbages and insufficient comprehensive benefits in the prior art. By combining the growth characteristics that the white-leaf No.1 tea tree variety and the middle-yellow No.1 tea tree variety grow slowly and grow weakly (plants are relatively short) in the North Sichuan tea area relative to general green tea and the actual construction of the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden, the high-yield cultivation system of the oxheart cabbage adaptive to the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden is constructed, and multiple beneficial effects are achieved. According to the method, the growth rhythm of'spring harvesting and summer cultivation 'of tea trees is accurately matched, relay intercropping is conducted after spring tea is harvested and pruned, the influence on spring tea core output is completely avoided, and the tea garden land and the light energy utilization rate are greatly improved.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

A product consisting of (a) 20-50% in weight of a coextract of Filipendula leaves and flowers (17.5-32.5%), Laurus leaves (17.5-32.5%), Brassica seeds (17.5-32.5%) and Withania roots (17.5-32.5%), (b) 49-80% in weight of a coextract of Cynara leaves (10-19%), Curcuma roots (29-55%) and Tanacetum flowers (29-55%); and (c) 0.6-1.2% in weight of an extract of Agave leaves. The extracts can be freeze-dried in water. Filipendula can be Filipendula ulmaria and / or Filipendula vulgaris. Laurus can be Laurus azorica and / or Laurus nobilis. Brassica can be Brassica rapa, Brassica nigra, Brassica oleracea, and / or Botrytis cymose. Withania can be Withania siniensis and / or Withania somnifera. Cynara can be Cynara cardunculus scolymus and / or Cynara flavescens. Curcuma can be Curcuma zedoaria and / or Curcuma longa. Tanacetum can be Tanacetum cinerariifolium, Tanacetum parthenium and / or Tanacetum vulgare. Agave can be Agave americana and / or Agave sisalana. A further aspect is a therapeutic adjuvant or composition comprising the product. The composition can comprise an anticancer e.g., chemotherapeutic. A further aspect is a method of treating cancer by administering the product. A further aspect is a method of assessing the product by measuring 14C activity, assessing the presence of miRNAs and exosomes, and assessing the functional resilience between different batches of the product.

The invention discloses a cabbage harvester which comprises a basic bearing frame, a cabbage harvesting device, an upward conveying mechanism, a backward conveying mechanism, a vegetable collecting box moving mechanism and a crawler-type walking mechanism. A diamond sand line root cutting mechanism is arranged on the cabbage harvesting device; according to the further improvement, a flexible supporting input mechanism is arranged above the cabbage harvesting device; an upper chain wheel conveying device is arranged above the lower chain wheel conveying device; in the upper chain wheel longitudinal circulating conveying belt, the second driving chains which are opposite at intervals are connected through a plurality of elastic belts; a long strip is spirally wound on the outer wall of the roller and is fixedly connected to the outer wall of the roller; the long strip is a hard plastic pipe; the vegetable collecting box movement mechanism is provided with a damage prevention structure and an automatic frame unloading device. The harvesting efficiency is remarkably improved, the loss of the brassica oleracea after the brassica oleracea is pulled to the ground is remarkably reduced, the conveying efficiency is remarkably improved through flexible wrapping, dead leaves on the brassica oleracea can be basically removed in the conveying process, trouble and time are saved, and damage to the brassica oleracea when the brassica oleracea falls into a vegetable collecting box is remarkably reduced.

The invention discloses a primer group for detecting xanthomonas campestris pv.campestris. The primer group consists of an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.2. The invention further discloses a kit for detecting the xanthomonas campestris pv.campestris. According to the present invention, multiple sequence comparison analysis is performed on the whole genome sequences of the Xanthomonas campestris pv. Campestris pathogen and other related bacteria, such that a pair of primers is designed according to the genome specificity region of the Xanthomonas campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris; the method is used for high-sensitivity rapid molecular detection of plants with the brassica oleracea, and a rapid, simple, high-specificity and high-sensitivity monitoring technology system for the brassica oleracea is established.

The patent discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO:1 to SEQ ID NO:4 in a sequence table. The primer group and the application of the molecular marker can efficiently and accurately identify the fertility of broccoli, solve the problem that 50% of fertile plants need to be pulled out in seed production, and reduce the cost of manpower and material resources.

The utility model belongs to the technical field of seedling raising of brassica oleracea, and particularly relates to a seedling raising box for planting brassica oleracea, which comprises a seedling raising box body, two supporting plates which are symmetrically arranged are fixedly connected to the interior of the seedling raising box body; the top of the supporting plate is fixedly connected with an L-shaped plate. The top of the supporting plate is slidably connected with a seedling raising box; the top of the L-shaped plate is in threaded connection with a lead screw. The end part of the screw rod is fixedly connected with a knob; the end, away from the knob, of the lead screw is rotationally connected with a pressing plate. The side wall of the L-shaped plate is slidably connected with two guide rods which are symmetrically arranged; the guide rod is fixedly connected to the side wall of the pressing plate; by means of the structure, the seedling raising box can be rapidly mounted or dismounted, when the seedling raising box is damaged, the seedling raising box can be conveniently maintained or replaced, a seedling raising person can conveniently conduct operations such as watering and fertilizing on seedlings through the detachable seedling raising box, the operation convenience and accuracy are effectively improved, damage to the seedlings is reduced, and therefore the seedling raising efficiency is improved.

The invention relates to a rapid induction and screening method for transgenic hairy roots of brassica napus independent of tissue culture, which mainly comprises the simple steps of accelerating germination, cutting roots, dipping bacteria, continuing accelerating germination, transplanting and the like, the method only needs simple moisturizing and accelerating germination, and can be used for rapidly inducing and screening transgenic hairy roots of brassica napus by utilizing the characteristics of agrobacterium rhizogenes K599 under the background of not carrying out tissue culture. According to the method, hairy roots are generated by direct induction of hypocotyls of brassica oleracea seedlings, and transgenic hairy roots are rapidly and visually screened according to colors by further utilizing a plant expression vector containing Ruby tag protein and are used for subsequent gene function verification. The method is simple to operate, low in cost and short in time consumption, avoids tedious sterile operation, and provides important technical support for molecular biology research of rape.