29 results about "Brassica oleracea" patented technology

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

The application discloses a molecular marker for distinguishing early and late flowering of Brassica oleracea and application thereof, wherein the nucleotide sequence of the molecular marker exists in the non-coding region of chromosome 4 of the genome of early flowering Brassica oleracea, and does not exist in the non-coding region of chromosome 4 of the genome of late flowering Brassica oleracea; based on the molecular marker, a primer pair Bf5 is further designed, DNA of the Brassica oleracea is amplified by using the primer pair, and early flowering varieties or materials of the Brassica oleracea can be screened according to the presence or absence of the target band. The molecular marker and the primer pair provided by the application can be used for rapidly detecting early flowering varieties and materials of the Brassica oleracea, time and resources are greatly saved, selection efficiency is greatly improved, and thus the breeding process is accelerated.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

The invention discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and the sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 4 in a sequence table. By applying the primer group and the molecular marker, the fertility of the broccoli can be efficiently and accurately identified, the problem that 50% of fertile plants need to be pulled out in seed production is solved, and the manpower and material resource cost is reduced.

The application belongs to the technical field of molecular breeding, and particularly relates to a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line. The application provides a SCAR marker for detecting a heterologous radish mitochondrial fragment in a Brassica oleracea Ogura cytoplasmic male sterile line, which comprises one or more or all of markers AC1-AC16; the nucleotide sequences of the AC1-AC16 are shown in SEQ ID NO: 1-16. The SCAR marker of the application has high specificity and good stability, and can accurately identify whether the corresponding heterologous radish mitochondrial fragment is contained in a to-be-tested Brassica oleracea cytoplasmic male sterile line and the relative content thereof, thereby being beneficial to early selection of the Brassica oleracea cytoplasmic male sterile line, improving breeding efficiency, and reducing breeding cost.

The invention belongs to the technical field of microorganisms, and discloses application of verticillium lecanii in promoting cabbage growth. The verticillium lecanii JMC-01 (the preservation number is CCTCC NO: M2018303) is successfully colonized in a cabbage plant through spray inoculation, the invention further proves that the endophytic colonization of the verticillium lecanii JMC-01 has an obvious improvement effect on agronomic characters such as the plant height and the root length of the cabbage, and the verticillium lecanii JMC-01 has a good application prospect in the aspects of promoting the growth of the cabbage plant and increasing the yield of the cabbage.

1. The name of the design product: packing bag (Brassica oleracea garlic hawthorn drink). 2. The use of the design product: a packing bag of a product. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: front view. 5. The design product is a thin product, and other views are omitted.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The invention discloses a method for high-yield cultivation of oxheart cabbages by using mountain tea garden conditions, relates to the technical field of cabbage planting, and aims to solve the problems of low resource utilization rate of northern-Sichuan mountain special tea gardens, poor cultivation suitability of spring-stubble oxheart cabbages and insufficient comprehensive benefits in the prior art. By combining the growth characteristics that the white-leaf No.1 tea tree variety and the middle-yellow No.1 tea tree variety grow slowly and grow weakly (plants are relatively short) in the North Sichuan tea area relative to general green tea and the actual construction of the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden, the high-yield cultivation system of the oxheart cabbage adaptive to the white-leaf No.1 tea garden and the middle-yellow No.1 tea garden is constructed, and multiple beneficial effects are achieved. According to the method, the growth rhythm of'spring harvesting and summer cultivation 'of tea trees is accurately matched, relay intercropping is conducted after spring tea is harvested and pruned, the influence on spring tea core output is completely avoided, and the tea garden land and the light energy utilization rate are greatly improved.

The invention belongs to the technical field of brassica plant breeding, and relates to a reagent and method for overcoming self-incompatibility of Chinese cabbage and application. After the Chinese cabbages enter the flowering phase, an EGCG (epigallocatechin gallate) solution is sprayed on the stigmas of the Chinese cabbages, so that the self-incompatibility of the Chinese cabbages can be weakened to different degrees. Experimental results show that after the EGCG is sprayed, the number of seeds obtained from each pod of the self-incompatible Chinese cabbage is increased to 17.8 from original 0. The used reagent EGCG does not pollute the environment, especially the soil environment, has small damage to plants, and can be applied to seed reproduction work of the Chinese cabbage self-incompatible line.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

This invention discloses a recessive nuclear male sterility gene. BoaGMS1 Application in regulating male development in Chinese kale. This invention screens recessive nuclear male sterility mutants from Chinese kale breeding and isolates the Chinese kale male fertility gene using forward genetics. BoaGMS1 Further experiments confirmed that BoaGMS1 A gene mutation resulted in recessive nuclear male sterility in Chinese kale. Meanwhile, targeting... BoaGMS1 Cosegregating molecular markers developed for male-sterile lines can be used for identifying fertility alleles in plants, screening target plants in marker-assisted breeding, and determining seed purity. Furthermore, they can be used in the development of kale. BoaGMS1 Male-sterile lines can be applied to the breeding and production of new hybrid varieties of Chinese kale. Therefore, the male fertility gene for Chinese kale provided by this invention... BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS1 BoaGMS It plays an important role in the utilization of heterosis and hybrid seed production of Chinese kale and even Brassica oleracea vegetables.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

The invention relates to the technical field of agricultural mechanization, in particular to a brassica oleracea harvesting header control method based on low-loss pulling and flexible clamping, which comprises the following steps: S1, collecting diameter data, shape and spatial position data of brassica oleracea entering a harvesting header area; s2, calculating the roller spacing of the current cabbage group; s3, calculating and dynamically adjusting the roller speed through an adaptive algorithm; s4, calculating a proper clamping force; s5, the tensioning force of the elastic tensioning device is adjusted; s6, on the basis of the S2 and the S3, the matching of the roller speed and the roller distance is controlled; and S7, calculating and adjusting the optimal angle and speed of the cabbages entering the conveying channel. By dynamically adjusting the roller speed, the roller distance and the clamping force and combining cabbage space position data, the angle of the conveying channel and the root cutting time are accurately controlled, damage to cabbages in the harvesting process is effectively reduced, the harvesting precision and efficiency are improved, and the intelligence and adaptability of the harvesting header are enhanced.

A product consisting of (a) 20-50% in weight of a coextract of Filipendula leaves and flowers (17.5-32.5%), Laurus leaves (17.5-32.5%), Brassica seeds (17.5-32.5%) and Withania roots (17.5-32.5%), (b) 49-80% in weight of a coextract of Cynara leaves (10-19%), Curcuma roots (29-55%) and Tanacetum flowers (29-55%); and (c) 0.6-1.2% in weight of an extract of Agave leaves. The extracts can be freeze-dried in water. Filipendula can be Filipendula ulmaria and / or Filipendula vulgaris. Laurus can be Laurus azorica and / or Laurus nobilis. Brassica can be Brassica rapa, Brassica nigra, Brassica oleracea, and / or Botrytis cymose. Withania can be Withania siniensis and / or Withania somnifera. Cynara can be Cynara cardunculus scolymus and / or Cynara flavescens. Curcuma can be Curcuma zedoaria and / or Curcuma longa. Tanacetum can be Tanacetum cinerariifolium, Tanacetum parthenium and / or Tanacetum vulgare. Agave can be Agave americana and / or Agave sisalana. A further aspect is a therapeutic adjuvant or composition comprising the product. The composition can comprise an anticancer e.g., chemotherapeutic. A further aspect is a method of treating cancer by administering the product. A further aspect is a method of assessing the product by measuring 14C activity, assessing the presence of miRNAs and exosomes, and assessing the functional resilience between different batches of the product.

The invention belongs to the technical field of molecular breeding, and particularly relates to a molecular marker and a primer group for detecting phenotypes of dominant nuclear gene female sterility (dominant female sterility, DGFS, Dominant Genic Feather Sterility for short) of cabbage, and application of the molecular marker and the primer group in identification of dominant female sterility single plants of the cabbage. The invention provides a linked molecular marker for detecting dominant female sterility phenotypes of cabbages. The linked molecular marker comprises molecular markers G1 and G2, the nucleotide sequences of the G1 and the G2 are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. The invention also provides a primer group for amplifying the molecular marker, the primer group comprises primers G1p and G2p, and the nucleotide sequences of the primer pair G1p and G2p are as shown in SEQ ID NO: 3-6. The primer group disclosed by the invention is strong in specificity and good in stability, and can accurately identify whether a single plant of a cabbage segregation population is in a female sterile phenotype or not, so that early breeding of dominant female sterile single plants of cabbages is realized, and the breeding efficiency is improved.

The invention discloses a cabbage harvester which comprises a basic bearing frame, a cabbage harvesting device, an upward conveying mechanism, a backward conveying mechanism, a vegetable collecting box moving mechanism and a crawler-type walking mechanism. A diamond sand line root cutting mechanism is arranged on the cabbage harvesting device; according to the further improvement, a flexible supporting input mechanism is arranged above the cabbage harvesting device; an upper chain wheel conveying device is arranged above the lower chain wheel conveying device; in the upper chain wheel longitudinal circulating conveying belt, the second driving chains which are opposite at intervals are connected through a plurality of elastic belts; a long strip is spirally wound on the outer wall of the roller and is fixedly connected to the outer wall of the roller; the long strip is a hard plastic pipe; the vegetable collecting box movement mechanism is provided with a damage prevention structure and an automatic frame unloading device. The harvesting efficiency is remarkably improved, the loss of the brassica oleracea after the brassica oleracea is pulled to the ground is remarkably reduced, the conveying efficiency is remarkably improved through flexible wrapping, dead leaves on the brassica oleracea can be basically removed in the conveying process, trouble and time are saved, and damage to the brassica oleracea when the brassica oleracea falls into a vegetable collecting box is remarkably reduced.

The utility model relates to the field of dehumidification, in particular to a cabbage seed particle dehumidification device which comprises a device body, a stirring assembly is arranged at the top of the device body, a discharging port is formed in the back of the device body, an air inlet pipe is arranged on one side of the top of the device body, and a feeding assembly is arranged on the other side of the top of the device body. An air inlet cavity is formed in the side wall of the device main body, an air outlet cavity is formed in the other side of the device main body, a bearing assembly is arranged in an inner cavity of the device main body, and the bearing assembly comprises a convex block, a bearing plate, a first cavity, a second cavity and an exhaust hole; the air inlet cavity and the air outlet cavity are formed in the device body, the air inlet cavity is communicated with the first cavity in the bearing assembly, the air inlet cavity can guide air into the first cavity, seed particles are dehumidified along the exhaust holes, and the stirring effect of the stirring assembly is matched, so that the dehumidification effect on the seed particles is improved.

The invention discloses a primer group for detecting xanthomonas campestris pv.campestris. The primer group consists of an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.2. The invention further discloses a kit for detecting the xanthomonas campestris pv.campestris. According to the present invention, multiple sequence comparison analysis is performed on the whole genome sequences of the Xanthomonas campestris pv. Campestris pathogen and other related bacteria, such that a pair of primers is designed according to the genome specificity region of the Xanthomonas campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris pv. Campestris; the method is used for high-sensitivity rapid molecular detection of plants with the brassica oleracea, and a rapid, simple, high-specificity and high-sensitivity monitoring technology system for the brassica oleracea is established.

The invention provides an application of a broccoli BoHXK2LF gene in improving the content of indole glucosinolate in broccoli and the resistance of broccoli to fusarium. The invention relates to an application of an overexpression BoHXK2LF gene. The application is any one of the following applications: A1) an application in improving the resistance of broccoli to fusarium; a2) application in cultivation of fusarium-resistant broccoli; a3) application in increasing the content of new glucose brassinolide in broccoli; a4) in increasing the content of total indole glucosinolate in broccoli; a5) application in increasing the content of total glucosinolate in broccoli; wherein the nucleotide sequence of the BoHXK2LF gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The resistance of broccoli plants to pathogenic fungi (such as fusarium layered) can be improved. The method has important theoretical significance and practical value for cultivating high-disease-resistance broccoli, reducing pesticide application and promoting agricultural sustainable development.

The invention relates to the technical field of food fermentation, in particular to a microbial fermentation probiotic seriflux beverage and a preparation method thereof. The feed additive is prepared from carrots, lilies, dandelions, endive, celery and brassica oleracea through microbial fermentation. The preparation method comprises the following steps: raw material preparation, vegetable pretreatment, shredding, cleaning, blanching, squeezing, fermentation preparation, sterilization, temperature control, slurry water primer addition, paste preparation, mixed fermentation of the paste and vegetables, sealing fermentation, post-treatment and storage, vacuum packaging, and preservation in a fresh-keeping storehouse. The beverage retains the flavor of traditional seriflux, realizes large-scale production through a standardized process, is long in shelf life and rich in probiotics, has the health-care functions of reducing uric acid, reducing blood pressure, helping digestion, clearing heat, detoxifying and the like, is suitable for daily drinking and cooking seasoning, strictly executes a sterilization and disinfection process in the whole preparation process, effectively avoids infectious microbe pollution, and is suitable for large-scale production. The product is ensured to meet the food safety hygienic standard, and the problem of poor hygienic conditions of the traditional seriflux is solved.

The invention discloses a three-dimensional growth simulation method, device and equipment for cabbages, a medium and a product, and relates to the technical field of agriculture, in particular to the technical field of data processing. The method comprises the following steps: for each leaf of a cabbage plant in each growth batch, determining a morphological transformation parameter of the leaf according to a current accumulated temperature, a leaf morphological built accumulated temperature and an appearing accumulated temperature; according to the blade type of the blade and the form transformation parameter, simulating the blade surface form of the blade to obtain a target blade surface form of the blade; simulating photosynthesis of the leaves to obtain absorbed photosynthetically active radiation of the leaves; and simulating the growth process of the cabbage plant according to the target leaf surface morphology of each leaf in the cabbage plant and the absorbed photosynthetically active radiation. By means of the technical scheme, simulation of the whole growth process of cabbage plants, especially the growth process of leaves is achieved.

The invention relates to the technical field of plant genetic engineering, and provides a cold-resistant gene BcACL5.2 of black cabbage, and the nucleotide sequence of the cold-resistant gene BcACL5.2 is as shown in SEQ ID NO.1. The invention also provides an overexpression vector containing the gene and a host cell. Meanwhile, the invention also provides an application of the gene BcACL5.2 in breeding for improving the cold tolerance of plants and a method for improving the cold tolerance of the plants. According to the invention, a key gene BcACL5.2 related to the cold resistance is screened from black cabbage, and the gene is over-expressed in an arabidopsis thaliana plant, so that the positive regulation of the gene on the cold resistance of the plant is verified, and the growth ability of the plant under low-temperature stress can be improved; the invention lays a foundation for cultivating a new plant variety with strong cold resistance, especially a new black cabbage variety, and has important value for expanding a black cabbage planting area and promoting industrial development.

The invention discloses a ganoderma lucidum composite health-care product and a preparation method thereof, and belongs to the technical field of health-care food, and the ganoderma lucidum composite health-care product comprises the following raw materials: ganoderma lucidum, rhizoma polygonati, red dates, American ginseng and brassica oleracea. The health-care food is prepared from the following raw materials in parts by weight: 30-50 parts of ganoderma lucidum powder, 10-20 parts of rhizoma polygonati powder, 15-25 parts of red date powder, 5-15 parts of American ginseng powder and 5-10 parts of brassica oleracea powder. The health-care food has comprehensive health-care effects: various health-care effects of ganoderma lucidum, rhizoma polygonati, red dates, American ginseng and brassica oleracea are organically integrated; the health-care tea has the effects of regulating immunity, resisting fatigue, delaying senescence, nourishing and strengthening the body, and has a more comprehensive health-care effect; the retention rate of effective components is high: through an optimized preparation process, the effective components of the raw materials are retained to the greatest extent, and the health-care effect of the product is improved; the preparation process is simple, the preparation method is simple and convenient to operate, industrial production is easy, and the production cost is relatively low.

The patent discloses a primer group for identifying a broccoli nuclear male sterility gene and application of a molecular marker, and belongs to the technical field of biology. The primer group comprises a first primer pair and a second primer pair, each primer pair comprises a forward primer and a reverse primer, and sequences of the forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the second primer pair and the reverse primer of the second primer pair are sequentially shown as SEQ ID NO:1 to SEQ ID NO:4 in a sequence table. The primer group and the application of the molecular marker can efficiently and accurately identify the fertility of broccoli, solve the problem that 50% of fertile plants need to be pulled out in seed production, and reduce the cost of manpower and material resources.

The application discloses a molecular marker linked to a bulbous stem enlargement gene of Brassica oleracea and application, and belongs to the technical field of molecular breeding. The application uses a small ball material of a breeding high-generation inbred line material of Brassica oleracea and an EMS mutant library thereof, obtains a chromosome region linked to the bulbous stem enlargement gene of Brassica oleracea based on BSA population positioning, and develops a molecular marker in a candidate interval. A KASP molecular marker is designed according to single base mutation of a candidate gene, and genotypes of 523 single plants are identified by using the marker, and a coincidence rate with a phenotype reaches 100%. The application can be directly used for molecular marker assisted breeding of the bulbous stem enlargement plant of Brassica oleracea, improves selection efficiency of breeding, and speeds up a breeding process; meanwhile, lays a foundation for cloning, function verification and metamorphic stem growth and development of the enlargement gene Bofse-1 and related researches.

The utility model belongs to the technical field of seedling raising of brassica oleracea, and particularly relates to a seedling raising box for planting brassica oleracea, which comprises a seedling raising box body, two supporting plates which are symmetrically arranged are fixedly connected to the interior of the seedling raising box body; the top of the supporting plate is fixedly connected with an L-shaped plate. The top of the supporting plate is slidably connected with a seedling raising box; the top of the L-shaped plate is in threaded connection with a lead screw. The end part of the screw rod is fixedly connected with a knob; the end, away from the knob, of the lead screw is rotationally connected with a pressing plate. The side wall of the L-shaped plate is slidably connected with two guide rods which are symmetrically arranged; the guide rod is fixedly connected to the side wall of the pressing plate; by means of the structure, the seedling raising box can be rapidly mounted or dismounted, when the seedling raising box is damaged, the seedling raising box can be conveniently maintained or replaced, a seedling raising person can conveniently conduct operations such as watering and fertilizing on seedlings through the detachable seedling raising box, the operation convenience and accuracy are effectively improved, damage to the seedlings is reduced, and therefore the seedling raising efficiency is improved.

The invention relates to a rapid induction and screening method for transgenic hairy roots of brassica napus independent of tissue culture, which mainly comprises the simple steps of accelerating germination, cutting roots, dipping bacteria, continuing accelerating germination, transplanting and the like, the method only needs simple moisturizing and accelerating germination, and can be used for rapidly inducing and screening transgenic hairy roots of brassica napus by utilizing the characteristics of agrobacterium rhizogenes K599 under the background of not carrying out tissue culture. According to the method, hairy roots are generated by direct induction of hypocotyls of brassica oleracea seedlings, and transgenic hairy roots are rapidly and visually screened according to colors by further utilizing a plant expression vector containing Ruby tag protein and are used for subsequent gene function verification. The method is simple to operate, low in cost and short in time consumption, avoids tedious sterile operation, and provides important technical support for molecular biology research of rape.

The application discloses a SNP_011 molecular marker of Brassica oleracea wax synthesis genes and application thereof. The SNP_011 molecular marker of the Brassica oleracea wax synthesis genes provided by the application is located at the position of 39,352,424 of the 8th chromosome of a Brassica napus genome, and when the base at the position is T, the leaves of the Brassica oleracea are gray-green and have wax powder; when the base at the position is C, the leaves of the Brassica oleracea are bright green and have no wax powder. The application further provides a specific primer combination for detecting the SNP_011 molecular marker of the Brassica oleracea wax synthesis genes. The SNP_011 molecular marker of the Brassica oleracea wax synthesis genes and the specific primer combination provided by the application can be used for screening and identifying whether natural genetic materials have the trait of wax powder synthesis, and have important theoretical and practical guiding significance for accelerating the genetic improvement process of Brassica oleracea breeding and improving the breeding selection efficiency.