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SSR labels of brassica oleracea red leaf gene Re and application thereof

A kale and red leaf gene technology is applied to the SSR marker and application field of the kale red leaf gene Re, which can solve the problems of inability to apply molecular markers, no molecular markers of the kale red leaf gene, and assisted selection breeding.

Inactive Publication Date: 2015-08-19
SHENYANG AGRI UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] At present, the molecular markers of the red leaf gene of kale have not been obtained, so it cannot be applied to molecular marker-assisted selection breeding

Method used

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  • SSR labels of brassica oleracea red leaf gene Re and application thereof
  • SSR labels of brassica oleracea red leaf gene Re and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: With kale red leaf gene Re Acquisition of linked simple repeat sequence (SSR) markers

[0036] 1. The construction of segregated groups

[0037] The female European series of red kale and the Nagoya series of white kale were purchased from Zhengzhou Golden Century Horticultural Materials Co., Ltd., and the red leaf kale DH series obtained from the European series of red kale through free microspore culture was used as the female parent and the Nagoya series The white leaf kale DH line obtained by free microspore culture was used as the male parent cross, and all the F1 generation plants obtained showed red leaves. After F1 selfed, the F2 generation segregation group was constructed. Among all 4,284 F2 generation individuals sown, 3,234 were red leaves and 1,050 were white leaves. The segregation ratio of leaf color genes complies with Mendel's law of inheritance 3:1.

[0038] Among them, the two parents were obtained by free microspore culture method from the F...

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PUM

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Abstract

The invention provides two SSR labels that located on both sides of the brassica oleracea red leaf gene Re and are closely linked to the gene, i.e. C9Z90 and C9Z94. The genetic distances between the two labels and the Re gene are 0.3cM and 2.0cM respectively. According to the nucleotide sequences of the two labels, two pairs of specific amplification primers are designed respectively for molecule assisted selection of brassica oleracea white leaf gene. In the assistant selection process, the two labels can be used at the same time, and have the characteristics of good repeatability, high reliability, low detection cost, and time saving and labor saving.

Description

technical field [0001] The present invention relates to kale red leaf gene Re Tightly linked Simple Sequence Repeats (Simple Sequence Repeats, SSR) markers and methods for obtaining them. Background technique [0002] Kale is a variety of Brassica oleracea, a biennial herb. It is native to the Mediterranean coast to Asia Minor, and is now widely cultivated as an ornamental plant, mainly distributed in temperate regions. [0003] The history of introduction and cultivation in my country is not long, but it has become the most widely used ornamental plant in winter and spring. Our research group has established a mature kale microspore culture technology system, and created a series of DH lines. In order to speed up the breeding process, it is necessary to develop molecular markers for the important ornamental traits of kale - red leaf traits. [0004] At present, there is no molecular marker for the red leaf gene of kale, so it cannot be applied to molecular marker-assist...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2525/151
Inventor 冯辉任杰刘志勇章云李承彧王玉刚冀瑞琴
Owner SHENYANG AGRI UNIV
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