56results about How to "Shortened childhood" patented technology

The invention discloses a method for shortening the juvenile period of apple hybrid seedlings, comprising the following steps of: culturing rootstock seedlings, culturing dwarf interstock seedlings, culturing apple hybrid seedlings, grafting seedlings at a high position, performing final-period management on trees and the like. According to the method, can be realized, and the semi-finished seedlings grafted at a high position after planting can blossom and fruit after growing to the 4th year, and have the fruiting plant rate up to 20% in the 5th year and up to 40% in the 6th year. Traditional seedlings need 8-10 years of juvenile period, so the juvenile period of the seedlings is shortened by 4 years; and compared with a traditional method, the plant rate after the juvenile period is greatly increased as well. According to the invention, measures of curing dwarf interstock seedlings early, sowing seedlings, taking the high-position buds of the seedlings and grafting the high-position buds to the dwarf interstock seedlings, and then planting, and other steps are adopted, and interstock grafting is performed on the apple seedlings, thus greatly shortening the juvenile period of the apple seedlings, shortening breeding process and fruiting time, increasing breeding efficiency, and saving lots of field management cost and resource investment simultaneously.

The invention discloses a seedling culturing and planting technology of carya illinoensis and relates to the field of planting of fruit trees. The planting method comprises the following steps: (1) constructing a foundation; (2) accelerating germination and breeding; (3) grafting; (4) managing grafted seedlings; and (5) carrying out field management. By reasonably selecting carya illinoensis breeding, grafting and after-survival management methods, a lot of high-quality seedlings are obtained and sufficient nutrient supply of stocks is guaranteed; the time of breeding nursery stocks through a grafting technology is greatly shortened; dwarfing and shorted juvenile phases of walnut trees are realized and the walnut trees are fruited in advance.

The invention discloses a Jiuqian plum tree engrafting and cultivating method, comprising the following steps: planting the cultivated annual bitter plum rootstock seedlings in the seedlings-raising land in rows, in each December to establish a bitter plum free-stock nursery; selecting the bearing tree vegetative-shoots of Jiuqian plum trees as the scion cultivation stock trees to establish a scion nursery; collecting the vegetative shoots on the scion stock trees as scions to engraft in the middle ten days of next February; promoting the growth and harvest of the Jiuqian plum tree using management practices, plant growth regulator, shaping and trimming. The engrafting and cultivating method can stunt the Jiuqian plum trees, reduce the juvenile phase, advance the harvest phase, increase the yield and realize the scale planting.

The invention provides Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants; the gene is acquired by subjecting cDNA as a template to PCR (polymerase chain reaction) amplification, wherein the cDNA is acquired by reverse transcription of total RNA of gingko female flowers. The gene has the advantages that the gene GbMADS9 is capable of controlling blossoming of plants and shortening juvenile phase of plants, and under induction of GA3, ABA, salt stress, drought stress and cold damage stress, the overexpressed gene GbMADS9 is capable of enhancing plant tolerance to osmotic stress; the gene is applicable to controlling the blossoming phase of plants.

The invention relates to a method for boosting pear hybrid seedlings to form flowers ahead of time, belonging to the field of crop breeding and cultivation. The hybrid seedlings with certain growth undergo branch drawing, shoots twisting, girdling, PP333 spraying in a single or combined manner when the new shoots of the pear trees stop growing, later, the flower beginning conditions of the plants are surveyed and statistics of the flowering rates is made. The above treatment measures can boost the hybrid seedlings to form flowers 1-2 years ahead of time, thus effectively shortening the juvenile phase of the hybrid progeny. The method has theoretical and practical significance in shortening the breeding period, reducing the land occupation period of the hybrid seedlings and labors for orchard management and improving the selecting and breeding efficiency of the new fruit tree varieties and has broad application prospect in fruit tree breeding.

The invention provides a cultivation method of a mandarin nucellus seedling nascent system. The method comprises the following steps of: screening varieties, namely selecting a mandarin nucellus seedling; performing top grafting; biologically preventing and controlling disease and insect damages in combination with matched cultivation, and the like. By the cultivation method, a tangerine nucellus seedling nascent system can be selected and cultivated, the conventional varieties with local characteristics are improved, mandarin quality is improved, competitive power is enhanced and a better economic benefit is achieved.

The invention discloses a phalaenopsis flowering gene PhalLFY and application thereof. The sequence of the phalaenopsis flowering gene PhalLFY is as shown in the specification from the 41 base to the 1462 base of SEQ ID NO.1, and the encoded protein amino acid sequence of the gene PhalLFY is as shown as SEQ ID NO.2. The homologous gene PhalLFY of the gene LFY is cloned from phalaenopsis for the first time, and capable of affecting plant growth and development, promoting precocious flowering of plants and promoting axillary buds and lateral buds to gown into flower buds as proved by tests. Therefore, based on the phalaenopsis flowering gene PhalLFY, an effective technical means is provided for improving plant growth process through genetic engineering and regulating the flowering time; the phalaenopsis flowering gene PhalLFY has wide application prospect and high economic value.

The invention relates to a flower forcing method for passion fruit seedlings. The method includes the steps that 1, the seedlings are cultured until the age of the seedlings is seven months, and the length of seedling bodies is about two meters; 2, agriculture trimming is performed two times, and growth of first-level vines and growth of second-level vines are promoted; 3, with leaf critical withering being limit control, water treatment is performed for 6-10 days; 4, growth regulation and fertilizer application treatment of plants are performed,paclobutrazol is used for spraying for 5 days, and rapin and monopotassium phosphate are used for mixed spraying. Within 7-15 days after growth regulation and fertilizer application treatment, namely, buds start to appear from the fourth month after transplanting, normal blossoming and fruit bearing are realized, flowering is earlier by about three months than that in planting of common passion fruit seedlings, and the flower forcing method issimple, easy to implement, low in popularization cost and remarkable in economic benefit.

The invention discloses a cultivation method capable of shortening the juvenile phase of loquats. The cultivation method comprises the following steps: performing seed direct sowing seedling culture or nutritional bag seedling culture, cultivating loquat double-layer trunk tree forms, applying fertilizer, applying a composite plant growth regulator and the like. The double-layer trunk tree form is cultivated by utilizing the characteristics that the loquat has high dryness, remarkable layer property, high germinating ability and high branching ability; furthermore, the composite plant growth regulator is sprayed on new shoots, and seedlings are promoted to develop towards the reproduction and growth direction. The cultivation method has relatively good and stable effect, the juvenile phase of the loquats is kept for 2.5 to 6 years, mainly for 3.5 to 4.5 years, the juvenile phase of the loquats is shortened by 2 to 3 years, and the problem that the juvenile phase of the loquats is long is effectively solved.

The invention discloses construction and optimization of a simple and efficient carya illinoinensis agrobacterium tumefaciens transformation system. The method comprises the following steps: carrying out streak culture on agrobacterium rhizogenes on a TY solid culture medium to obtain single colonies, selecting the single colonies, and culturing the single colonies in a TY liquid culture medium to obtain an activated agrobacterium rhizogenes solution; infecting hypocotyl or stems, close to seed parts, of carya illinoensis seedlingswith agrobacterium rhizogenes, wherein the positions of hypocotyls or stems, close to the seed parts, of the carya illinoensis seedlings are 1-5 cm away from the hypocotyls or stems of the roots of the seedlings; and carrying out co-culture and rooting culture until transgenic hairy roots of the carya illinoensis grow at the positions where hypocotyls or stems are infected. The seedlings are used as propagation materials for infection, and a series of tissue culture technologies such as culture of aseptic seedlings in an aseptic environment for subculture operation are not needed.

The invention discloses an apple breeding method. The method comprises the steps as follows: scions are sheared into short shoots which are 5-10 cm long and have two buds; roots of the scions are cutinto smooth long slopes about 2.5 cm long by a knife from one side where no buds exist, one side (scion ends) where the buds exist is cut into flat wedges, and the scion ends are placed in a homemadesoaking solution to be soaked for 6-8 h; dwarfed self-rooted stocks or dwarfed interstocks are selected to form self-rooted nutrient stocks by laying propagation, upper ends of the stocks are shearedinto flat openings, and cuts with depth of about 2.5-3 cm are longitudinally formed downwards from branches at about 1/4 positions of cut sections; scions are inserted into stock cuts, so that the scions and the stocks are closely attached without seams, layers are aligned to each other, and 0.2 cm of upper ends of scion cutting surfaces are exposed for binding. The graft survival rate reaches 95%or above, meanwhile, apple trees grow fast after grafting, crowns take shape and bear fruits early, and the juvenile period can be shortened by 2-3 years, so that better economic benefits can be created.

The invention relates to the field of plant cultivation, in particular to a flowering induction method for dendrobium nobile seedlings. The method adopts the combination of hormone induction, temperature and nutritional regulation, the problems are solved that the flowering period of the dendrobium nobile seedlings is long, and flowering is not uniform. By adopting suitable hormone concentration,spraying frequency, spraying time and reasonable low-temperature treatment temperature and time and by utilizing the C/N ratio and beneficial components of liquid-state carbon-contained compound fertilizer, the combination of nutrition regulation is conducted to induct flowering of the dendrobium nobile seedlings, multiple pseudo bulbs of dendrobium nobile can be inducted to flower, the initial flowering period is short, the quantity of flowers is large, flowering is regular, ornamental value is high, and the new method is provided for cultivation of competitive product dendrobium nobile.

The invention discloses a precursor gene of hickory micro159a. The sequence of the precursor gene is: (a) a nucleotide sequence shown as SEQ ID NO:1, or (b) a nucleotide sequence which has at least 70percent, at least 75 percent, at least 80 percent, at least 85 percent, at least 90 percent, at least 95 percent, at least 96 percent, at least 97 percent, at least 98 percent or at least 99 percentof homology with the nucleotide sequence shown as SEQ ID NO:1 and can be used for generating the hickory micro159a after cutting. The application of the precursor sequence of the hickory micro159a isexpected to advance the plant flowering artificially. Specifically, the application of the hickory micro159a is expected to regulate the flowering period of hickory, shorten the childhood of hickory and advance flowering and fruiting.

The invention discloses an apocarya planting technology, and relates to the field of fruit tree planting. The planting method comprises the following steps of 1 base construction, 2 germination accelerating and seedling growing, 3 grafting, 4 grafted seedling management and 5 field management. By reasonably selecting the apocarya seedling growing, grafting and survival post-management methods, a large number of high-quality seedlings are obtained, sufficient nutrient supply of stock is ensured, nursery stock breeding time spent through the grafting technology is greatly shortened, dwarfing ofapocarya trees is achieved, the juvenile phase is shortened, and fruiting is achieved ahead of time.

A new technology for flowering promoting of kaffir lily is characterized by including the steps of preparing culture soil, selectively using fertilizer and adjusting illumination. By means of the processes of low-temperature processing, temperature varying, light increasing and the like, the juvenile phase of the kaffir lily is shortened by 2-3 years, and scape appears 2-3 times or even 4-5 timesevery year. The technology is suitable for planting for flowering promoting of kaffir lily.