184 results about "Flowering time" patented technology

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention relates to an application of an arabidopsis gene IDD14, an arabidopsis gene IDD15 and an arabidopsis gene IDD16 in regulating and controlling the flowering time of plants. We find that three mutants with deletion of IDD14, IDD15 and IDD16 bloom in advance, overexpression plants of the three mutants are delayed in blooming, which indicates that a subfamily composed of IDD14, IDD15 and IDD16 participates in blooming regulation and has a negative regulation function which is different from the positive blooming regulation function of other IDD members, so that a new gene resource is provided for the application of IDD in breeding, and the application of IDD14, IDD15 and IDD16 in breeding is promoted. And a support is provided for research on flowering time regulation of grain crops such as corn, rice, wheat and the like and other crops.

The invention discloses application of a blue light receptor gene ZmZTLb in regulation and control of flowering time of corn, and belongs to the technical field of biology. According to the invention, corn ZmZTLb gene knockout and overexpression strains are respectively obtained through a CRISPER / Cas9 technology and a gene engineering technology, the flowering time of different strains under natural long and short sunshine is further verified, and it is proved that the flowering period of the ZmZTLb knockout strain is obviously advanced compared with that of a wild type under the condition of natural long sunshine, and the yield of the ZmZTLb gene knockout strain is obviously increased. And the flowering period of the ZmZTLb overexpression strain is obviously delayed under the conditions of long sunlight and short sunlight. Meanwhile, the invention reveals that the accumulation of ZmZTLb protein in corn is induced by blue light, after the corn ZmZTLb is knocked out, the expression quantity of flowering genes such as ZCN7, ZCN8 and ZCN12 is obviously increased, and the tasseling period, the spinning period and the pollen scattering period of the corn are obviously shortened, so that the flowering time is obviously advanced. The invention provides a theoretical basis for innovation of corn germplasm resources.

The invention discloses an application of a litchi LcCPC3 gene and an encoded protein thereof in delaying flowering of plants. The nucleotide sequence of the litchi LcCPC3 gene is shown as SEQ ID NO: 1; the amino acid sequence of the encoded protein is shown as SEQ ID NO: 2. According to the invention, the litchi LcCPC3 gene participates in regulating and controlling the flowering time of litchis and is an important regulating and controlling gene for flowering of the litchis, the litchi LcCPC3 gene can be used for regulating and controlling the flowering time of the litchis and stably flowering, and the litchi LcCPC3 gene has important application value for realizing stable yield of the litchis.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention relates to the technical field of biology, in particular to a tobacco NtATC3 / 4 gene and application of the tobacco NtATC3 / 4 gene to regulation and control of plant flowering and leaf surface area. The function of the NtATC3 / 4 gene is researched by using a virus induced gene silencing (VIGS) technology, and the result shows that after the NtATC3 / 4 gene is silenced, the flowering time of tobacco can be delayed, the leaf surface area can be increased and / or the stem length can be reduced. The results show that the flowering time of tobacco can be controlled by regulating and controlling the expression quantity of the NtATC3 / 4 gene, so that early flowering is prevented and controlled, the yield reduction caused by cold and drought stress is reduced, the leaf surface area is increased, and the income of tobacco growers is increased, and therefore, the NtATC3 / 4 gene has a wide market application prospect.

The invention discloses an alfalfa growth period and quality related protein MsCOG1 as well as a coding gene and application thereof. The Dof transcription factor is separated from medicago sativa, the amino acid sequence of the Dof transcription factor is as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene of the Dof transcription factor is as shown in SEQ ID No.1. According to the invention, the coding gene of the Dof transcription factor in medicago sativa is over-expressed in medicago sativa, so that compared with a wild type, the obtained transgenic medicago sativa material has the advantages that the flowering time is obviously delayed, the contents of nutritional ingredients such as crude protein and trace elements are obviously increased, and the contents of acid detergent fibers, neutral detergent fibers and lignin are obviously reduced; the relative feeding value and the relative feeding quality are obviously improved. The invention provides a gene resource for molecular breeding and genetic engineering improvement of forage grass, and has application prospects in the aspects of cultivating high-quality and high-yield forage grass varieties or controlling the flowering period of the forage grass, improving the quality of the forage grass and the like.

This invention discloses the application of the BnaTOE1 gene in regulating the flowering time of Brassica napus and its molecular marker primers for identification. The DNA nucleotide sequence of the BnaTOE1 gene is shown in SEQ ID NO.1, with a sequence length of 1254 bp, and the encoded amino acid sequence is shown in SEQ ID NO.2. The molecular marker primers used to identify the flowering time of Brassica napus are shown in SEQ ID NO.15 and SEQ ID NO.16. Functional analysis of genetic transformation in Brassica napus shows that BnaTOE1 participates in regulating the flowering time of Brassica napus. The development of molecular markers is of great significance for promoting the genetic improvement and breeding of Brassica napus.

The invention belongs to the technical field of gene engineering, and particularly relates to a cornutgiella axataenae PdMYB44 gene and application thereof. The invention provides a cornutgiella dotacea PdMYB44 gene, wherein the CDS (Coding Sequence) of the PdMYB44 gene is as shown in SEQ ID NO. 1. The PdMYB44 gene disclosed by the invention is derived from the cornutgiium axataenense, so that the drought resistance of a transgenic plant can be improved, the flowering time is shortened, and the PdMYB44 gene can be applied to plant stress resistance genetic improvement. Results of embodiments show that according to a transgenic plant over-expressing the PdMYB44 gene, the proline content is increased, the malondialdehyde content is reduced, the transpiration rate and stomatal conductance are remarkably reduced, and the drought resistance of the transgenic plant is jointly improved by synergistically regulating three key mechanisms, namely accumulation of permeation protection substances, inhibition of membrane damage and optimization of stomatal behaviors.

The present invention relates to the field of biotechnology and relates to a kind of alfalfa MsCAL Gene, and its application, MsCAL The nucleotide sequence of the gene is shown in SEQ ID No.29. MsCAL The gene is specifically expressed in the plant stem apex, with the highest expression level in the floral organs during the reproductive growth stage, indicating that it is specifically involved in the plant flowering process. MsCAL Gene overexpression caused transgenic Arabidopsis to flower early, become dwarfed, and have fewer rosette leaves. In addition, the transgenic Arabidopsis changed from an indeterminate inflorescence to a finite inflorescence. Quantitative results of known genes regulating flowering time and floral organ development in Arabidopsis showed that MsCAL It can regulate the expression of downstream flowering and flower organ development related genes. MsCAL The expression delayed flowering, reduced crude fiber and lignin content, and increased forage digestibility and crude protein content.

A method for producing a plant with flowering-time regulation, the method comprising introducing a mutation into the EMF3 gene in a plant.

This invention belongs to the field of biological agents and plant breeding technology, specifically relating to the application of *Trichoderma tumefaciens* T-51 in promoting flowering, fruit setting, and vegetative growth in plants. The *Trichoderma tumefaciens* T-51 described in this invention has the preservation number CCTCCNO: M2015729. Applying a suspension containing *Trichoderma tumefaciens* T-51 to the roots of plant seedlings can advance the flowering time and increase the number of flowers; it can also improve the number and rate of fruit set; and it can promote vegetative growth, especially increasing plant height, number of stem nodes, and number of leaves, thereby accelerating the plant breeding process and shortening the breeding cycle. This invention has very high application value in plant breeding.

The application discloses application of a PRR5 gene in regulating early flowering of soybean under high temperature, and belongs to the field of genetic engineering. The application is directed to a soybean PRR5 gene, and a double mutant prr5a prr5b in which PRR5a shown in a nucleotide sequence as shown in SEQ ID NO. 1 and PRR5b shown in a nucleotide sequence as shown in SEQ ID NO. 2 are simultaneously knocked out is constructed by using CRISPR technology. Experimental results show that the double mutant has a flowering time under high temperature (30 DEG C) consistent with a flowering time under normal temperature (25 DEG C), and the response mechanism of the soybean in which the PRR5 gene is knocked out is relieved from early flowering under high temperature. The application provides a new idea, technical means and biological material for changing early flowering of the soybean under high temperature and enhancing production management of the soybean under high temperature. The application has a wide application prospect in research and high-yield breeding of the soybean in response to high temperature stress, and is helpful to promoting development of a soybean breeding industry.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The present invention discloses a PeRAV1 gene of moso bamboo, its expression protein and application, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the PeRAV1 gene of moso bamboo disclosed in the present invention is shown as SEQ ID NO.1, and the amino acid sequence of its expression protein is shown as SEQ ID NO.2. The present invention constructs an overexpression vector of the PeRAV1 gene of moso bamboo; transforms the constructed overexpression vector of the PeRAV1 gene of moso bamboo into Arabidopsis thaliana; cultivates, screens and obtains transgenic Arabidopsis thaliana with delayed flowering time and / or increased number of rosette leaves. The results of the examples of the present application show that the strains overexpressing the PeRAV1 gene exhibit obvious late flowering phenotypes, increased number of rosette leaves and delayed flowering time; and the expression levels of downstream regulatory genes FT, SOC1 and GA3OX1 are significantly downregulated.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention discloses a phyllostachys edulis PeAKIN3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys pubescens PeAKIN3 gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence contained in the polypeptide coded by the phyllostachys pubescens PeAKIN3 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an overexpression vector of a phyllostachys edulis PeAKIN3 gene; the constructed overexpression vector of the phyllostachys edulis PeAKIN3 gene is transformed into arabidopsis thaliana; and culturing and screening to obtain the transgenic arabidopsis thaliana strain. The invention discloses the function of the PeAKIN3 gene for the first time, and compared with a control group, transgenic arabidopsis thaliana with overexpression of the phyllostachys edulis PeAKIN3 gene shows obvious late flower phenotype, the number of rosette leaves is increased, and the flowering time is delayed, which indicates that the PeAKIN3 can prolong the vegetative growth period of the plant and postpone the reproductive growth of the plant.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a Chinese rose SVP transcription factor in regulation and control of flowering phase and petal size. The invention aims to provide a new choice for regulating Chinese rose organs by researching MADS-box transcription factor family member SVP in Chinese rose. The technical scheme of the invention is the application of the Chinese rose SVP transcription factor in regulating and controlling the flowering phase and / or the petal size of a plant. The amino acid sequence of the SVP transcription factor is shown as SEQ ID NO.2. By adopting the method disclosed by the invention, accurate intervention on the petal size and the flowering phase of the Chinese rose can be realized through RhSVP silencing.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1 and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

The present invention relates to the field of plant genetic engineering technology, and in particular to a wheat cold-resistant gene TaERF7-like and its application. The present invention provides an application of a wheat ethylene response element binding protein family AP2 / EREBP transcription factor gene TaERF7-like in plant cold resistance and advancing the flowering time of plants. Plants transformed with this gene can tolerate low temperature stress and advance the flowering time. The nucleotide sequence of the gene and the amino acid sequence of the encoded protein are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively. By overexpressing the gene in Arabidopsis thaliana, the cold resistance of the transgenic plants can be significantly improved, and the flowering time of the plants can be advanced. The TaERF7-like gene of the present invention can be used as a genetic resource for genetic improvement of crops and has broad application prospects.

The application provides a method for shortening the growth cycle of lettuce, and belongs to the technical field of lettuce breeding. The method for shortening the growth cycle of lettuce comprises the following steps: after planting the lettuce, high-temperature treatment is performed for 20-22 days, and the conditions of the high-temperature treatment are as follows: 30 DEG C for 4 hours during the day, 40 DEG C for 4 hours during the day, 30 DEG C for 8 hours during the day, and 25 DEG C for 8 hours at night. The application promotes the lettuce to enter the reproductive growth stage in advance through high-temperature stress, can significantly shorten the present budding time and the initial flowering time of the lettuce, and can shorten the present budding time and the initial flowering time of the lettuce and the lettuce by 4-6 weeks, so that the growth cycle of the lettuce is shortened. The method is simple and easy to operate, and can be popularized and planted on a large scale.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flowering time trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flowering time trait, and to methods of producing plants having a flowering time trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flowering time trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flowering time trait of interest or for modulating the flowering time of cannabis plants.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1, E2, E3, E4, E1La and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

The invention discloses a method for improving the hybridization survival rate of soybeans in different ecological regions in Hainan and application, and belongs to the technical field of soybean breeding. The method effectively solves the problem of low soybean hybridization survival rate caused by short sunlight and low temperature conditions in Hainan and special structures of soybean petals, also solves the technical problem that when soybean varieties in different ecological regions are planted in Hainan at the same time, the growth periods are different, and the flowering periods cannot meet each other, greatly shortens the flowering period, prolongs the flowering time, and improves the yield. Effective hybridization of soybean varieties in different ecological regions in Hainan is achieved, the hybridization survival rate is increased to 60% or above, compared with 37% of a traditional mode, the hybridization survival rate is greatly increased, and the purpose of multiple hybridization in one year is achieved.

The invention belongs to the field of plant genes, and relates to application and a method of a GmTOC1a gene or protein in regulation and control of soybean flowering. The application of the soybean biological clock gene GmTOC1a in flowering control is found, two homologous copies GmTOC1a1 (Glyma. 05G025000) and GmTOC1a2 (Glyma. 17G102200) of the GmTOC1a gene are directionally knocked out through a CRISPR / Cas9 gene editing technology, a soybean mutant with the GmTOC1a gene mutated is obtained, research finds that the mutant shortens the growth period of soybeans and advances the flowering time, and it is indicated that the GmTOC1a inhibits soybean flowering. Therefore, the GmTOC1a gene can be applied to change or expand the cultivation regionalism of soybeans, so that the soybeans can be sown at different latitudes, the planting region of the soybeans is expanded, and a theoretical basis and genetic resources are provided for breeding good varieties of the soybeans.

The application discloses application of a GhELF3 gene in regulating flowering time of plants. The application provides application of any one of the following 1)-3) in regulating flowering time, bolting time and / or rosette leaf number of plants; 1) protein GhELF3; 2) a nucleic acid molecule coding protein GhELF3; and 3) a recombinant carrier, an expression cassette or a recombinant bacterium containing the nucleic acid molecule coding protein GhELF3. A cotton biological clock gene GhELF3 is cloned from Gossypium hirsutum, and the GhELF3 is an orthologous gene of Arabidopsis thaliana AtELF3. The GhELF3 shows an oscillation expression mode within one day, and an expression amount of the GhELF3 in a late-flowering cotton variety is significantly higher than that in an early-flowering cotton variety. An overexpression vector of the gene is constructed, and Arabidopsis thaliana is transformed by a dipping method. It is found that the flowering time of the transgenic Arabidopsis thaliana overexpressing the GhELF3 is obviously later than that of the wild-type Arabidopsis thaliana.

The invention belongs to the technical field of agriculture, and particularly relates to a nitrogen regulation and control method for shortening the growth period of rice. According to the test, the regulation and control effect of short-term nitrogen concentration dynamic change (not the constant nitrogen level in the traditional research) on the flowering time of the rice is explored, and the special treatment mode of changing the nitrogen concentration from high to low (HTL) in the booting stage can more effectively promote the rice to bloom in advance (3 days shortened) compared with constant high nitrogen or constant low nitrogen) and keep the high yield. The nitrogen regulation and control method for shortening the growth period of the rice comprises the following steps that first-stage nitrogen treatment is provided at the front stage of the booting stage of the rice, second-stage nitrogen treatment is provided at the rear stage of the booting stage of the rice, in the booting stage of the rice, stem end growth cones are differentiated to flag leaf pillows to expose tips, and during the first-stage nitrogen treatment, the second-stage nitrogen treatment is carried out; the usage amount of the nitrogen fertilizer is 0.5-1 mM; during the second-stage nitrogen treatment, the usage amount of the nitrogen fertilizer is 6-8 mM.

The present invention relates to the technical field of plant genetic engineering and provides DIV1 the application of a gene in regulating the flowering time of plants. DIV1 The application of the gene is in at least one of the following S1 to S2: S1, regulating the flowering time of plants; S2, cultivating transgenic plants. By the above technical solution, the problems in the related art that the flowering time of plants is late, the growth period is long, and it is difficult to adapt to various farming systems are solved.