126 results about "Flowering time" patented technology

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention relates to the technical field of biology, in particular to a tobacco NtATC3 / 4 gene and application of the tobacco NtATC3 / 4 gene to regulation and control of plant flowering and leaf surface area. The function of the NtATC3 / 4 gene is researched by using a virus induced gene silencing (VIGS) technology, and the result shows that after the NtATC3 / 4 gene is silenced, the flowering time of tobacco can be delayed, the leaf surface area can be increased and / or the stem length can be reduced. The results show that the flowering time of tobacco can be controlled by regulating and controlling the expression quantity of the NtATC3 / 4 gene, so that early flowering is prevented and controlled, the yield reduction caused by cold and drought stress is reduced, the leaf surface area is increased, and the income of tobacco growers is increased, and therefore, the NtATC3 / 4 gene has a wide market application prospect.

The invention discloses an alfalfa growth period and quality related protein MsCOG1 as well as a coding gene and application thereof. The Dof transcription factor is separated from medicago sativa, the amino acid sequence of the Dof transcription factor is as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene of the Dof transcription factor is as shown in SEQ ID No.1. According to the invention, the coding gene of the Dof transcription factor in medicago sativa is over-expressed in medicago sativa, so that compared with a wild type, the obtained transgenic medicago sativa material has the advantages that the flowering time is obviously delayed, the contents of nutritional ingredients such as crude protein and trace elements are obviously increased, and the contents of acid detergent fibers, neutral detergent fibers and lignin are obviously reduced; the relative feeding value and the relative feeding quality are obviously improved. The invention provides a gene resource for molecular breeding and genetic engineering improvement of forage grass, and has application prospects in the aspects of cultivating high-quality and high-yield forage grass varieties or controlling the flowering period of the forage grass, improving the quality of the forage grass and the like.

This invention discloses the application of the BnaTOE1 gene in regulating the flowering time of Brassica napus and its molecular marker primers for identification. The DNA nucleotide sequence of the BnaTOE1 gene is shown in SEQ ID NO.1, with a sequence length of 1254 bp, and the encoded amino acid sequence is shown in SEQ ID NO.2. The molecular marker primers used to identify the flowering time of Brassica napus are shown in SEQ ID NO.15 and SEQ ID NO.16. Functional analysis of genetic transformation in Brassica napus shows that BnaTOE1 participates in regulating the flowering time of Brassica napus. The development of molecular markers is of great significance for promoting the genetic improvement and breeding of Brassica napus.

The invention belongs to the technical field of gene engineering, and particularly relates to a cornutgiella axataenae PdMYB44 gene and application thereof. The invention provides a cornutgiella dotacea PdMYB44 gene, wherein the CDS (Coding Sequence) of the PdMYB44 gene is as shown in SEQ ID NO. 1. The PdMYB44 gene disclosed by the invention is derived from the cornutgiium axataenense, so that the drought resistance of a transgenic plant can be improved, the flowering time is shortened, and the PdMYB44 gene can be applied to plant stress resistance genetic improvement. Results of embodiments show that according to a transgenic plant over-expressing the PdMYB44 gene, the proline content is increased, the malondialdehyde content is reduced, the transpiration rate and stomatal conductance are remarkably reduced, and the drought resistance of the transgenic plant is jointly improved by synergistically regulating three key mechanisms, namely accumulation of permeation protection substances, inhibition of membrane damage and optimization of stomatal behaviors.

A method for producing a plant with flowering-time regulation, the method comprising introducing a mutation into the EMF3 gene in a plant.

The application discloses application of a PRR5 gene in regulating early flowering of soybean under high temperature, and belongs to the field of genetic engineering. The application is directed to a soybean PRR5 gene, and a double mutant prr5a prr5b in which PRR5a shown in a nucleotide sequence as shown in SEQ ID NO. 1 and PRR5b shown in a nucleotide sequence as shown in SEQ ID NO. 2 are simultaneously knocked out is constructed by using CRISPR technology. Experimental results show that the double mutant has a flowering time under high temperature (30 DEG C) consistent with a flowering time under normal temperature (25 DEG C), and the response mechanism of the soybean in which the PRR5 gene is knocked out is relieved from early flowering under high temperature. The application provides a new idea, technical means and biological material for changing early flowering of the soybean under high temperature and enhancing production management of the soybean under high temperature. The application has a wide application prospect in research and high-yield breeding of the soybean in response to high temperature stress, and is helpful to promoting development of a soybean breeding industry.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The invention discloses a phyllostachys edulis PeAKIN3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys pubescens PeAKIN3 gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence contained in the polypeptide coded by the phyllostachys pubescens PeAKIN3 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an overexpression vector of a phyllostachys edulis PeAKIN3 gene; the constructed overexpression vector of the phyllostachys edulis PeAKIN3 gene is transformed into arabidopsis thaliana; and culturing and screening to obtain the transgenic arabidopsis thaliana strain. The invention discloses the function of the PeAKIN3 gene for the first time, and compared with a control group, transgenic arabidopsis thaliana with overexpression of the phyllostachys edulis PeAKIN3 gene shows obvious late flower phenotype, the number of rosette leaves is increased, and the flowering time is delayed, which indicates that the PeAKIN3 can prolong the vegetative growth period of the plant and postpone the reproductive growth of the plant.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a Chinese rose SVP transcription factor in regulation and control of flowering phase and petal size. The invention aims to provide a new choice for regulating Chinese rose organs by researching MADS-box transcription factor family member SVP in Chinese rose. The technical scheme of the invention is the application of the Chinese rose SVP transcription factor in regulating and controlling the flowering phase and / or the petal size of a plant. The amino acid sequence of the SVP transcription factor is shown as SEQ ID NO.2. By adopting the method disclosed by the invention, accurate intervention on the petal size and the flowering phase of the Chinese rose can be realized through RhSVP silencing.

The application provides a method for shortening the growth cycle of lettuce, and belongs to the technical field of lettuce breeding. The method for shortening the growth cycle of lettuce comprises the following steps: after planting the lettuce, high-temperature treatment is performed for 20-22 days, and the conditions of the high-temperature treatment are as follows: 30 DEG C for 4 hours during the day, 40 DEG C for 4 hours during the day, 30 DEG C for 8 hours during the day, and 25 DEG C for 8 hours at night. The application promotes the lettuce to enter the reproductive growth stage in advance through high-temperature stress, can significantly shorten the present budding time and the initial flowering time of the lettuce, and can shorten the present budding time and the initial flowering time of the lettuce and the lettuce by 4-6 weeks, so that the growth cycle of the lettuce is shortened. The method is simple and easy to operate, and can be popularized and planted on a large scale.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flowering time trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flowering time trait, and to methods of producing plants having a flowering time trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flowering time trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flowering time trait of interest or for modulating the flowering time of cannabis plants.

The invention discloses a method for improving the hybridization survival rate of soybeans in different ecological regions in Hainan and application, and belongs to the technical field of soybean breeding. The method effectively solves the problem of low soybean hybridization survival rate caused by short sunlight and low temperature conditions in Hainan and special structures of soybean petals, also solves the technical problem that when soybean varieties in different ecological regions are planted in Hainan at the same time, the growth periods are different, and the flowering periods cannot meet each other, greatly shortens the flowering period, prolongs the flowering time, and improves the yield. Effective hybridization of soybean varieties in different ecological regions in Hainan is achieved, the hybridization survival rate is increased to 60% or above, compared with 37% of a traditional mode, the hybridization survival rate is greatly increased, and the purpose of multiple hybridization in one year is achieved.

The invention belongs to the field of plant genes, and relates to application and a method of a GmTOC1a gene or protein in regulation and control of soybean flowering. The application of the soybean biological clock gene GmTOC1a in flowering control is found, two homologous copies GmTOC1a1 (Glyma. 05G025000) and GmTOC1a2 (Glyma. 17G102200) of the GmTOC1a gene are directionally knocked out through a CRISPR / Cas9 gene editing technology, a soybean mutant with the GmTOC1a gene mutated is obtained, research finds that the mutant shortens the growth period of soybeans and advances the flowering time, and it is indicated that the GmTOC1a inhibits soybean flowering. Therefore, the GmTOC1a gene can be applied to change or expand the cultivation regionalism of soybeans, so that the soybeans can be sown at different latitudes, the planting region of the soybeans is expanded, and a theoretical basis and genetic resources are provided for breeding good varieties of the soybeans.

The application discloses application of a GhELF3 gene in regulating flowering time of plants. The application provides application of any one of the following 1)-3) in regulating flowering time, bolting time and / or rosette leaf number of plants; 1) protein GhELF3; 2) a nucleic acid molecule coding protein GhELF3; and 3) a recombinant carrier, an expression cassette or a recombinant bacterium containing the nucleic acid molecule coding protein GhELF3. A cotton biological clock gene GhELF3 is cloned from Gossypium hirsutum, and the GhELF3 is an orthologous gene of Arabidopsis thaliana AtELF3. The GhELF3 shows an oscillation expression mode within one day, and an expression amount of the GhELF3 in a late-flowering cotton variety is significantly higher than that in an early-flowering cotton variety. An overexpression vector of the gene is constructed, and Arabidopsis thaliana is transformed by a dipping method. It is found that the flowering time of the transgenic Arabidopsis thaliana overexpressing the GhELF3 is obviously later than that of the wild-type Arabidopsis thaliana.

The invention belongs to the technical field of agriculture, and particularly relates to a nitrogen regulation and control method for shortening the growth period of rice. According to the test, the regulation and control effect of short-term nitrogen concentration dynamic change (not the constant nitrogen level in the traditional research) on the flowering time of the rice is explored, and the special treatment mode of changing the nitrogen concentration from high to low (HTL) in the booting stage can more effectively promote the rice to bloom in advance (3 days shortened) compared with constant high nitrogen or constant low nitrogen) and keep the high yield. The nitrogen regulation and control method for shortening the growth period of the rice comprises the following steps that first-stage nitrogen treatment is provided at the front stage of the booting stage of the rice, second-stage nitrogen treatment is provided at the rear stage of the booting stage of the rice, in the booting stage of the rice, stem end growth cones are differentiated to flag leaf pillows to expose tips, and during the first-stage nitrogen treatment, the second-stage nitrogen treatment is carried out; the usage amount of the nitrogen fertilizer is 0.5-1 mM; during the second-stage nitrogen treatment, the usage amount of the nitrogen fertilizer is 6-8 mM.

The invention discloses a passion fruit PeBBX24 gene and application of the passion fruit PeBBX24 gene in regulation and control of plant early blossoming, and belongs to the technical field of gene engineering. The nucleotide sequence of a coding region of the passion fruit PeBBX24 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the protein coded by the passion fruit PeBBX24 gene is as shown in SEQ ID NO.2. Experiments prove that the heterologous overexpression passion fruit PeBBX24 gene in arabidopsis thaliana can obviously promote early flowering, the flowering time is advanced by 5-6 days, and the early flowering phenotype can be stably expressed under the conditions of medium and long sunshine and short sunshine, so that the gene has relatively strong adaptability to different light environments, and the application range of the gene under various cultivation conditions is widened. The gene can up-regulate the expression quantity of flowering promoting factors CO, FT, SOC1 and FUL and down-regulate the expression quantity of flowering inhibiting factors SVP and FLC, so that flowering transformation is effectively promoted, and early flowering regulation is realized. The gene can be used for cultivating passion fruit, arabidopsis thaliana and other early blossoming transgenic plants, and has important application value for shortening the breeding period and improving the breeding efficiency.

The invention discloses a phyllostachys edulis PeRD22 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys edulis PeRD22 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the phyllostachys edulis PeRD22 gene is as shown in SEQ ID NO.2. According to the invention, an overexpression vector of the PeRD22 gene is constructed and transformed into arabidopsis thaliana, and a transgenic plant of the PeRD22 gene is obtained through culture. The result shows that the flowering time of a PeRD22 gene overexpressed plant is delayed compared with that of a control plant, the number of rosette leaves is increased, and an obvious late flowering phenotype is shown, which indicates that the PeRD22 gene negatively regulates the flowering of the plant. The PeRD22 gene disclosed by the invention can be used for regulating and controlling the flowering phase of plants and is beneficial to breeding of high-quality bamboo plant varieties.

The invention relates to application of a GbPIF3 gene functional structure domain in plant type regulation, which is characterized in that a plant type related gene Gb-PIF3 is obtained by cloning from an apical meristem in a hybrid F1 of sea island cotton-upland cotton, and the functional structure domain GbPIL of the gene is subjected to heterologous expression in arabidopsis thaliana through a transgenic technology. The photoresponse test and seedling property observation on the obtained transgenic material prove that the gene functional structure domain has the function in plant type regulation and control, can be used for transforming the plant type structure of upland cotton and regulating and controlling the flowering time, and has important significance on the improvement of the yield and quality of cotton, especially the genetic modification of close-planting cotton.

This invention relates to a method for creating late-flowering, high-yielding alfalfa, characterized by the following steps: S1: designing and synthesizing targeted alfalfa MvFT S1: Construct the gRNA of the gene; S2: Construct a CRISPR / Cas9 gene knockout vector containing the gRNA expression cassette described in S1; S3: Transform the knockout vector described in S2 into Agrobacterium tumefaciens; S4: Transform explants of alfalfa cultivars into Agrobacterium tumefaciens as described in S3; S5: Screen, differentiate, and regenerate the explants transformed in S4 to obtain transgenic plants; S6: Perform molecular and phenotypic identification on the transgenic plants to screen for... MvFT A late-flowering, high-yielding germplasm with homozygous gene knockout and significantly delayed flowering time. This invention reveals for the first time... MvFT The key functions of genes in the regulation of flowering in alfalfa were investigated, and new late-flowering, high-yielding alfalfa germplasm was successfully created, providing valuable germplasm resources for breeding new high-yielding and high-quality alfalfa varieties.

The invention relates to the technical field of bioengineering, and provides a chrysanthemum G protein coupled receptor GCR or related factors thereof and application thereof in flower development regulation. Specifically, the invention provides a method for regulating chrysanthemum flower development, the method is carried out by regulating expression of CmGCR and / or CmGCR related factors, and the CmGCR related factors are downstream factors regulated by CmGCR in a photoperiod pathway, downstream factors regulated by CmGCR in a hormone signal pathway or downstream factors regulated by CmGCR in floral organ development. The invention also provides an application of the CmGCR and / or the CmGCR related factor in regulating and controlling the flowering time of the plant. New functions of the CmGCR and the CmGCR related factors are applied to flower development regulation and control, and the inflorescence development process and the flowering time can be accurately regulated, so that the CmGCR is particularly suitable for improving germplasm in the flowering phase and creating early-flowering or late-flowering germplasm.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The invention provides a mango flowering regulation gene MiABI5-like7 and application thereof, and belongs to the technical field of gene engineering. The invention discovers a new application of the MiABI5-like7 gene in regulating and controlling the flowering of plants, and discovers that the bolting and the flowering period of arabidopsis thaliana are obviously advanced and the quantity of rosette leaves is obviously reduced by heterologous overexpression of the MiABI5-like7 gene in arabidopsis thaliana, which indicates that the gene has the function of promoting the flowering of arabidopsis thaliana. Through silent expression of the MiABI5-like7 gene in mango buds, it is found that the conversion time of the mango flower buds is obviously delayed, the green tip rate (initial flower bud rate) is obviously reduced, it is indicated that silencing of the gene has the function of delaying formation of the mango flower buds, and therefore the MiABI5-like7 gene can also be used for regulating and controlling the flowering time of mangoes.

The invention belongs to the field of durian cultivation, and particularly relates to a durian flower forcing agent, a preparation method and application. According to the durian flower forcing agent, prohexadione calcium, potassium phosphite, benzyl benzyl aminopurine, thidiazuron and ethephon are reasonably combined, durian can be promoted to bloom in advance, the durian flower forcing agent is diluted and then sprayed to durian trees, budding of the durian trees can be achieved 9 days after spraying, and the budding time and the blooming time are shortened; meanwhile, the cluster number of durian flower buds can be remarkably increased, important guarantee is provided for increasing the yield of durian, and economic benefits of durian cultivation can be greatly increased.

This invention discloses a method for increasing the yield of hybrid japonica rice seed production. Belonging to the field of rice seed production technology, the specific steps are as follows: conducting germination tests on the male-sterile and restorer lines of hybrid japonica rice; confirming the sowing time; soaking, germinating, and sowing the seeds; transplanting to a multi-span greenhouse; spraying water and applying temperature stimulation when the male-sterile line flowers, and closing the top film-rolling device; spraying water and applying temperature stimulation when both the male-sterile and restorer lines flower, ensuring complete coverage; after the panicles are sun-dried, they flower again, and the top film-rolling device is opened to facilitate pollination. This invention precisely regulates the flowering time of hybrid japonica rice, promoting earlier flowering of the male-sterile and restorer lines, allowing them to flower simultaneously. Both the glenching angle of the male-sterile line and the pollen quantity of the restorer line are highly concentrated, improving pollination efficiency, increasing the cross-pollination rate of the male-sterile line and the seed production yield, and providing stable working hours, greatly improving individual work efficiency.