305 results about "Flowering time" patented technology

The invention relates to the field of gene editing, in particular to a peony PoLEC1 gene and application of protein coded by the peony PoLEC1 gene. The peony PoLEC1 gene is cloned from cultivated peony paeonia ostii, after the peony PoLEC1 gene is transformed into arabidopsis thaliana, phenotypic contrast finds that the peony PoLEC1 gene can play a role in bolting and flowering as well as growth and development of plants, specifically, overexpression of the peony PoLEC1 gene can increase the number of leaves, delay bolting and flowering time and reduce the height of the plants. According to the invention, arabidopsis thaliana is taken as a model plant, and a certain theoretical basis is provided for establishing a complete plant regeneration and genetic transformation system for peony by exploring the functional characteristics of the peony PoLEC1 gene and the expression characteristics of the peony PoLEC1 gene in the whole somatic embryo direct generation process and in each tissue of overexpressed arabidopsis thaliana.

The invention relates to the technical field of biology, and particularly provides a method for controlling soybean plant height and / or delaying soybean flowering time and application of the method. The method for controlling the plant height of the soybean comprises the following steps: knocking out or overexpressing a GmFTIP26 gene in the soybean; the GmFTIP26 gene has a nucleotide sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The method for delaying the flowering time of the soybeans comprises the following steps: knocking out a GmFTIP26 gene in the soybeans; the GmFTIP26 gene has a nucleotide sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the technical scheme, the GmFTIP26 gene in the soybean is knocked out or overexpressed, the plant height of the soybean is regulated and / or the flowering time of the soybean is delayed, so that the plant is more suitable for the local growth environment, and the yield and the quality of the soybean are improved.

The invention discloses a Chinese orchid flowering regulation gene CsFTL3 as well as an encoding protein and application thereof, and belongs to the field of plant molecular biology. The gene CsFTL3 for regulating and controlling flowering of Chinese cymbidium is separated from flower bud cDNA (complementary deoxyribonucleic acid) of a Chinese cymbidium variety 'Xiaoxiang', and 174 amino acids are encoded by the gene CsFTL3. Through construction of an expression vector of the CsFTL3 gene, overexpression in wild-type arabidopsis thaliana and heterologous overexpression of the CsFTL3 gene in the arabidopsis thaliana, it is found that bolting and flowering periods of the arabidopsis thaliana are significantly delayed, flower stems are significantly thickened and increased, the number of rosette leaves is significantly increased, and the growth period is prolonged, which indicates that the gene has the function of delaying the flowering time of the arabidopsis thaliana. The Chinese orchid flowering regulation and control gene can be used for molecular mechanism research of orchid flowering time, delay Chinese orchid flowering time, promote plant vegetative growth, prolong whole Chinese orchid viewing time and promote Chinese orchid genetic improvement and variety breeding.

The invention belongs to the technical field of plant molecular biology, and particularly relates to an oilseed rape flowering negative regulation transcription factor BnMYB615, a cloning method, a BnMYB615 gene overexpression vector, a BnMYB615 gene knockout vector, a host cell, a flowering phase delay transgenic plant and application of a flowering phase advance gene editing plant. A nucleotide sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.1, an amino acid sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.2, an overexpression vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.3, and a gene knockout vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.4. The overexpressed BnMYB615 can obviously delay the flowering time of plants, and can prolong the growth season and the photosynthetic time of the plants and obtain higher yield when being applied to crops taking nutritive organs as harvest objects; after the BnMYB615 gene is knocked out, the flowering time of the plant is advanced, and when the BnMYB615 gene is applied to crops taking seeds or fruits as harvest objects, the growth period of the crops can be shortened, the multiple cropping index can be improved, meanwhile, the influence of adverse climate can be avoided, and the product quality can be improved.

The invention belongs to the field of gene engineering, and relates to a mutant of a corn auxin ex-carrier gene ZmPGP1 and application of the mutant. By editing the corn ZmPGP1 gene, the regulation and control effects of the corn ZmPGP1 gene on the corn plant height, the flowering time and the disease resistance are determined, and finally a mutant BR2CR7 which is relatively mild in dwarfing phenotype, has a late flowering phenotype and does not influence the yield is obtained. Compared with a wild type KN5585, the BR2CR7 mutant has the advantages that the plant height of corn is effectively reduced, dwarfing is realized, and rational close planting can be carried out; the flowering time can be regulated and controlled, the pollen scattering time becomes late, and yield reduction caused by rainy seasons can be avoided; compared with a wild type BR2CR7 mutant, the BR2CR7 mutant can effectively resist various pathogenic bacteria under the natural growth condition, the occurrence probability of yellow leaves and yellow spots is reduced, and the survival rate and yield of corn are guaranteed. Therefore, the BR2CR7 mutant disclosed by the invention can be widely used for cultivating new corn varieties.

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention relates to an application of an arabidopsis gene IDD14, an arabidopsis gene IDD15 and an arabidopsis gene IDD16 in regulating and controlling the flowering time of plants. We find that three mutants with deletion of IDD14, IDD15 and IDD16 bloom in advance, overexpression plants of the three mutants are delayed in blooming, which indicates that a subfamily composed of IDD14, IDD15 and IDD16 participates in blooming regulation and has a negative regulation function which is different from the positive blooming regulation function of other IDD members, so that a new gene resource is provided for the application of IDD in breeding, and the application of IDD14, IDD15 and IDD16 in breeding is promoted. And a support is provided for research on flowering time regulation of grain crops such as corn, rice, wheat and the like and other crops.

Methods for decreasing flowering time and accelerating crop cycle in plants and increasing the number of breeding cycles per year in such plants are provided. The methods comprise using far-red light alone or in combination with additional external signals such as photoperiod in order to decrease flowering time and accelerate seed to seed crop cycle. Also provided are methods for bypassing vernalization requirements in winter annuals.

Described herein is a florigen pathway toolkit that comprises mutations in genes in the florigen pathway in Solanaceae, such as mutations in genes in the florigen pathway in tomatoes, that are useful to quantitatively modulate or adjust the balance of opposing flowering signals to customize and improve shoot architecture and flowering (flowering time) for tomato breeding, particularly for fresh market tomato breeding.

The invention belongs to the technical field of tea cultivation, and particularly relates to a flowering delaying function of a tea tree gene CsCIP1 and application of the tea tree gene CsCIP1. According to the invention, a sequence CsTGY12G0001995 homologous with the AtCIP1 gene of Arabidopsis thaliana is found in a tea tree, and the sequence CsTGY12G0001995 is named as CsCIP1. After the gene is overexpressed in arabidopsis thaliana, the flowering time of a transgenic plant is later than that of a wild plant, which proves that the gene has the function of delaying flowering and provides a candidate gene for directionally cultivating a flowering-delayed tea tree variety. The tea tree gene CsCIP1 and the flowering delaying function analysis of the tea tree gene CsCIP1 are beneficial to providing important gene resources and powerful theoretical support for improving tea tree varieties in agricultural production, and have wide application value.

The invention discloses application of a gene MtPrx38 in regulation and control of growth, development and quality of forage grass. The method comprises the following steps: carrying out genome PCR (Polymerase Chain Reaction) identification to obtain homozygotes MtPrx38-1 and MtPrx38-2 of the medicago truncatula MtPrx38 gene mutant; the expression quantity of the MtPrx38 gene detected by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) is obviously reduced, and compared with wild medicago truncatula, the peroxidase activity of the mutant homozygotes MtPrx38-1 and MtPrx38-2 is obviously reduced. Compared with wild medicago truncatula, the MtPrx38 gene mutant has the advantages that the root length, the lateral root number, the plant height, the branch number, the leaf area and the secondary branch number are obviously increased; the flowering time is advanced; the lignin content and the cellulose content are obviously reduced, which indicates that the gene MtPrx38 has the functions of regulating and controlling the growth, development and quality of forage grass. The method has application prospects in the aspects of promoting growth and development of forage grass, improving forage grass quality and the like.

The invention discloses application of a blue light receptor gene ZmZTLb in regulation and control of flowering time of corn, and belongs to the technical field of biology. According to the invention, corn ZmZTLb gene knockout and overexpression strains are respectively obtained through a CRISPER / Cas9 technology and a gene engineering technology, the flowering time of different strains under natural long and short sunshine is further verified, and it is proved that the flowering period of the ZmZTLb knockout strain is obviously advanced compared with that of a wild type under the condition of natural long sunshine, and the yield of the ZmZTLb gene knockout strain is obviously increased. And the flowering period of the ZmZTLb overexpression strain is obviously delayed under the conditions of long sunlight and short sunlight. Meanwhile, the invention reveals that the accumulation of ZmZTLb protein in corn is induced by blue light, after the corn ZmZTLb is knocked out, the expression quantity of flowering genes such as ZCN7, ZCN8 and ZCN12 is obviously increased, and the tasseling period, the spinning period and the pollen scattering period of the corn are obviously shortened, so that the flowering time is obviously advanced. The invention provides a theoretical basis for innovation of corn germplasm resources.

The invention discloses an application of a litchi LcCPC3 gene and an encoded protein thereof in delaying flowering of plants. The nucleotide sequence of the litchi LcCPC3 gene is shown as SEQ ID NO: 1; the amino acid sequence of the encoded protein is shown as SEQ ID NO: 2. According to the invention, the litchi LcCPC3 gene participates in regulating and controlling the flowering time of litchis and is an important regulating and controlling gene for flowering of the litchis, the litchi LcCPC3 gene can be used for regulating and controlling the flowering time of the litchis and stably flowering, and the litchi LcCPC3 gene has important application value for realizing stable yield of the litchis.

The invention discloses application of a BnaTOE1 gene in regulating flowering time of brassica napus and a molecular marker primer for identification of the BnaTOE1 gene. The DNA nucleotide sequence of the BnaTOE1 gene is as shown in SEQ ID NO.1, the sequence length is 1254bp, the coded amino acid sequence is as shown in SEQ ID NO.2, and molecular marker primers for identifying the flowering phase of the brassica napus are as shown in SEQ ID NO.15 and SEQ ID NO.16. The invention further discloses a molecular marker for identifying the flowering phase of the brassica napus. Functional analysis of brassica napus genetic transformation shows that BnaTOE1 participates in regulating and controlling the flowering time of brassica napus, and development of the molecular marker has important significance in promoting genetic improvement and breeding of brassica napus.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides transcription factor CtWRKY41 and application of the cynanchum thesioides transcription factor CtWRKY41 in improving plant salt tolerance and delaying flowering time. The cynanchum thesioides transcription factor CtWRKY41 provided by the invention is a transcription factor of a WRKY gene family capable of improving the salt tolerance of plants, the salt tolerance of the plants or the yeast can be remarkably improved by expressing the coding gene CtWRKY41 in the plants or the yeast, and a technical support is provided for deeply analyzing the biological functions of the WRKY gene family and improving the stress resistance of crops. Furthermore, the invention also finds that the cynanchum thesioides transcription factor CtWRKY41 can also reduce the sensitivity of plants to exogenous abscisic acid, delay the flowering time of the plants, delay the bolting time of the plants and increase the quantity of rosette leaves of the plants, thereby providing a new biological resource for crop improvement.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention provides a brassica napus BnaC3. LEAFY early blossoming molecular marker and application thereof in breeding, and belongs to the technical field of plant molecular breeding and gene editing. Aiming at gene structure variation analysis of a large fragment on a genome of a brassica napus core germplasm resource, genetic loci influencing inflorescence formation are excavated, and the early blossoming molecular marker is used for early blossoming of the brassica napus BnaC3. LEAFY early blossoming of the brassica napus BnaC3. LEAFY early blossoming. And a corresponding gene structure variation sequence DEL00110133 is obtained. A molecular marker is developed on the basis of the structural variation sequence, and the obtained molecular marker can be used for screening germplasm with the characters of early inflorescence establishment and early flowering time in different germplasm resources of the cabbage type rape, so that subsequent rapid breeding of the rape is facilitated. According to the invention, CRISPR / Cas9-based genetic sequence editing is carried out on the gene structure variation sequence to obtain a new late flowering genetic sequence, and a new method and a new gene sequence resource are provided for breeding of brassica napus with late inflorescence establishment and late flowering time.

Compositions and methods for altering the flowering and / or maturing time of soybean plants are provided. Compositions include isolated and recombinant polynucleotides encoding polypeptides, expression cassettes, host cells, plants, plant parts that stabilize incorporation of these polynucleotides. Methods and kits for producing these plants via transgenic means, breeding, or genome editing methods and identifying plants with altered flowering and / or maturation times are provided.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention relates to the technical field of biology, in particular to a tobacco NtATC3 / 4 gene and application of the tobacco NtATC3 / 4 gene to regulation and control of plant flowering and leaf surface area. The function of the NtATC3 / 4 gene is researched by using a virus induced gene silencing (VIGS) technology, and the result shows that after the NtATC3 / 4 gene is silenced, the flowering time of tobacco can be delayed, the leaf surface area can be increased and / or the stem length can be reduced. The results show that the flowering time of tobacco can be controlled by regulating and controlling the expression quantity of the NtATC3 / 4 gene, so that early flowering is prevented and controlled, the yield reduction caused by cold and drought stress is reduced, the leaf surface area is increased, and the income of tobacco growers is increased, and therefore, the NtATC3 / 4 gene has a wide market application prospect.

The invention discloses an alfalfa growth period and quality related protein MsCOG1 as well as a coding gene and application thereof. The Dof transcription factor is separated from medicago sativa, the amino acid sequence of the Dof transcription factor is as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene of the Dof transcription factor is as shown in SEQ ID No.1. According to the invention, the coding gene of the Dof transcription factor in medicago sativa is over-expressed in medicago sativa, so that compared with a wild type, the obtained transgenic medicago sativa material has the advantages that the flowering time is obviously delayed, the contents of nutritional ingredients such as crude protein and trace elements are obviously increased, and the contents of acid detergent fibers, neutral detergent fibers and lignin are obviously reduced; the relative feeding value and the relative feeding quality are obviously improved. The invention provides a gene resource for molecular breeding and genetic engineering improvement of forage grass, and has application prospects in the aspects of cultivating high-quality and high-yield forage grass varieties or controlling the flowering period of the forage grass, improving the quality of the forage grass and the like.

The present invention provides a BnaC3.LEAFY early flowering molecular marker of Brassica napus and its application in breeding, belonging to the field of plant molecular breeding and gene editing technology, and analyzes the gene structure variation of large fragments on the genome of the core germplasm resources of Brassica napus, excavates the genetic loci that affect the early and late formation of inflorescence, and obtains the corresponding gene structure variation sequence DEL00110133; molecular markers are developed based on the structure variation sequence, and the obtained molecular markers can be used to screen the germplasm with early inflorescence establishment and early flowering time traits in different germplasm resources of Brassica napus, which is beneficial to the subsequent rapid breeding of rapeseed. The present invention also performs genetic sequence editing based on CRISPR / Cas9 on the gene structure variation sequence to obtain a new late flowering genetic sequence, and provides a new method and new gene sequence resources for the breeding of Brassica napus with late inflorescence establishment and late flowering time.

The present invention discloses a major effect QTL locus of chromosome C06 related to the flowering time of Brassica napus, an InDel molecular marker and an application thereof, and belongs to the field of molecular biology. The nucleotide sequence of the molecular marker is shown in SEQ ID NO: 3 and SEQ ID NO: 4. Experimental results show that the strain carrying the sequence of SEQ ID NO: 3 has an earlier flowering time, and the strain carrying the sequence of SEQ ID NO: 4 has a later flowering time. The major effect QTL locus of chromosome C06 of the present invention has a higher contribution rate to the flowering time trait of Brassica napus, and plays a key role in regulating the flowering time trait of Brassica napus. The molecular markers closely linked thereto can assist in the selection of early flowering materials and cultivate early flowering and early maturing rapeseed. The molecular markers have the advantages of good accuracy, high efficiency and high cost performance, and are suitable for large-scale promotion and application. The present invention is helpful for the cloning of genes related to the flowering time of rapeseed and the development of functional markers of genes related to the flowering time.

This invention discloses the application of the BnaTOE1 gene in regulating the flowering time of Brassica napus and its molecular marker primers for identification. The DNA nucleotide sequence of the BnaTOE1 gene is shown in SEQ ID NO.1, with a sequence length of 1254 bp, and the encoded amino acid sequence is shown in SEQ ID NO.2. The molecular marker primers used to identify the flowering time of Brassica napus are shown in SEQ ID NO.15 and SEQ ID NO.16. Functional analysis of genetic transformation in Brassica napus shows that BnaTOE1 participates in regulating the flowering time of Brassica napus. The development of molecular markers is of great significance for promoting the genetic improvement and breeding of Brassica napus.

The invention belongs to the technical field of gene engineering, and particularly relates to a cornutgiella axataenae PdMYB44 gene and application thereof. The invention provides a cornutgiella dotacea PdMYB44 gene, wherein the CDS (Coding Sequence) of the PdMYB44 gene is as shown in SEQ ID NO. 1. The PdMYB44 gene disclosed by the invention is derived from the cornutgiium axataenense, so that the drought resistance of a transgenic plant can be improved, the flowering time is shortened, and the PdMYB44 gene can be applied to plant stress resistance genetic improvement. Results of embodiments show that according to a transgenic plant over-expressing the PdMYB44 gene, the proline content is increased, the malondialdehyde content is reduced, the transpiration rate and stomatal conductance are remarkably reduced, and the drought resistance of the transgenic plant is jointly improved by synergistically regulating three key mechanisms, namely accumulation of permeation protection substances, inhibition of membrane damage and optimization of stomatal behaviors.

The present invention relates to the field of biotechnology and relates to a kind of alfalfa MsCAL Gene, and its application, MsCAL The nucleotide sequence of the gene is shown in SEQ ID No.29. MsCAL The gene is specifically expressed in the plant stem apex, with the highest expression level in the floral organs during the reproductive growth stage, indicating that it is specifically involved in the plant flowering process. MsCAL Gene overexpression caused transgenic Arabidopsis to flower early, become dwarfed, and have fewer rosette leaves. In addition, the transgenic Arabidopsis changed from an indeterminate inflorescence to a finite inflorescence. Quantitative results of known genes regulating flowering time and floral organ development in Arabidopsis showed that MsCAL It can regulate the expression of downstream flowering and flower organ development related genes. MsCAL The expression delayed flowering, reduced crude fiber and lignin content, and increased forage digestibility and crude protein content.

A method for producing a plant with flowering-time regulation, the method comprising introducing a mutation into the EMF3 gene in a plant.

The present invention relates to the field of gene editing, and particularly to the application of the peony PoLEC1 gene and the protein encoded thereby. The peony PoLEC1 gene was cloned from the cultivated peony 'Fengdan' in the present invention. After transforming it into Arabidopsis thaliana, through phenotypic comparison, it was found that the peony PoLEC1 gene can play a role in the bolting, flowering, growth and development of plants, specifically manifested in that overexpression of the peony PoLEC1 gene can increase the number of leaves, delay the bolting and flowering time and reduce the plant height. Taking Arabidopsis thaliana as a model plant, by exploring the functional characteristics of the peony PoLEC1 gene and its expression characteristics in the whole process of direct somatic embryogenesis and various tissues of overexpressed Arabidopsis thaliana, a certain theoretical basis is provided for establishing a complete plant regeneration and genetic transformation system for peonies.

This invention belongs to the field of biological agents and plant breeding technology, specifically relating to the application of *Trichoderma tumefaciens* T-51 in promoting flowering, fruit setting, and vegetative growth in plants. The *Trichoderma tumefaciens* T-51 described in this invention has the preservation number CCTCCNO: M2015729. Applying a suspension containing *Trichoderma tumefaciens* T-51 to the roots of plant seedlings can advance the flowering time and increase the number of flowers; it can also improve the number and rate of fruit set; and it can promote vegetative growth, especially increasing plant height, number of stem nodes, and number of leaves, thereby accelerating the plant breeding process and shortening the breeding cycle. This invention has very high application value in plant breeding.