9 results about "Ectopic expression" patented technology

The invention belongs to the technical field of genetic engineering and biosynthesis, and particularly relates to a streptomyces-sourced siderophore-polyketone hybrid product synthesis related gene cluster, a genetically engineered bacterium and application of the genetically engineered bacterium. The invention provides an NIS siderophore synthetase gene cluster. The gene cluster is selected from a gene cluster BGC19 or a gene cluster desB. Meanwhile, the invention discloses two biological heterologous or ectopic expression engineering bacteria for producing the hybrid siderophore and application of the siderophore-polyketone hybrid compound by taking streptomyces as a host, and discloses structures and activities of the siderophore-polyketone hybrid compound produced by the two engineering bacteria. The invention provides a scheme for the construction of engineering bacteria for generating new hybrid compounds by utilizing the expression of non-adjacent gene clusters at specific sites and functional combination among the gene clusters, also provides a new element for the biosynthesis of a novel siderophore and polyketone combination, and has good practical application value.

The application belongs to the technical field of plant genetic engineering and genetic breeding, and particularly relates to a method for improving regeneration and genetic transformation efficiency of peanut plants by ectopic expression of a gene AhLEC1a The application discloses a method for improving regeneration efficiency and genetic transformation success rate of peanut plants by ectopic expression of a gene AhLEC1a The method is characterized in that a beta-estradiol inducible expression vector is constructed AhLEC1a The transgenic plants are obtained by using somatic embryo regeneration or a direct organ regeneration approach. AhLEC1a The ectopic expression of the gene can significantly improve somatic embryo induction rate (up to 100%) and the number of cells of a single explant (2.13 times of that of a control group); the explant bud induction efficiency of the direct organ regeneration approach is improved by 22% compared with that of the control group; and the transgenic positive rates of the two methods are improved by 27.81% and 6.15% respectively compared with those of the control group. The application breaks through the difficulties of low regeneration efficiency and low transformation success rate in peanut genetic transformation, and provides important technical support for peanut gene function research and molecular breeding.

Helper innate lymphoid cells (ILC) play important functions in immunity and tissue homeostasis, but their development remains poorly understood. In this study, the inventors investigated the precise function of the transcription factor NFIL3, which they established as the earliest requirement during ILC development in mouse bone marrow. Using NFIL3 retroviral ectopic expression in all-lymphoid progenitors, they showed that NFIL3 triggers a developmental process that recapitulates in vitro natural ILC development, in the absence of any other instructive signal. The present invention relates to an in vitro method for obtaining innate lymphoid precursors cells, said method comprising the step of i) culturing hematopoietic precursors cells in an appropriate culture medium, and ii) manipulating the said hematopoietic precursors cells to increase the expression or function of the Nuclear Factor Interleukin (3) Regulated (NFIL3).

This invention belongs to the field of plant genetic engineering and molecular breeding technology, specifically relating to the application of wheat TabHLH93 protein or its encoding gene in regulating plant grain development. This invention constructs a structure capable of ectopic expression... TabHLH93 A binary expression vector for the gene was genetically transformed into common wheat. TabHLH93 Offspring with high gene expression achieved TabHLH93 The gene was overexpressed and verified, demonstrating that TabHLH93 regulates wheat grain size and plays a significant role in regulating agronomic traits such as plant height, tiller number, spike length, and thousand-grain weight. This provides a promising candidate gene for high-yield genetic improvement of wheat, which is of great importance for ensuring food security.

The present invention provides methods of transdifferentiating a somatic cell, e.g., a fibroblast, to an induced dopaminergic precursor cell (iDP) in vitro. The methods include ectopically expressing genes of (1) one or both of Brn2 and Brn4, or a variant thereof, (2) Sox2, or a variant thereof, and (3) one or both of Foxa2 and Lmx1a, or a variant thereof, in the somatic cell, and methods for treating a neurodegenerative disease, such as Parkinson's disease, with the iDP.

Expression cassettes comprising FOXP3 proteins, chimeric receptors, and / or cell surface receptors are provided. Also provided herein are Treg cells comprising the expression cassettes and methods of use.

The present invention relates to a modified crop plant or part thereof which exhibits increased or ectopic expression of one or more one transcription factors, which one or more transcription factors are specifically expressed in the plant's ESR and / or in the plant's BETL, or modulate gene expression of one or more ESR and / or BETL-specific genes.

The present disclosure provides a chimeric antigen receptor (CAR) immunotherapy for treating a hematologic cancer comprising (a) genetically modifying a population of immune effector cells comprising T cell receptors (TCRs) to stably express at least one CAR, wherein the ectodomain of the CAR specifically binds a cancer antigen; (b) expanding the population of CAR-containing immune effector cells in the presence of one or more cytokines in vitro to achieve a therapeutic dose, wherein the population of CAR-containing immune effector cells exhibits a nonexhausted memory T cell phenotype; and (c) infusing eligible subjects with the CAR-containing immune effector memory T cell phenotype population of cells as needed until the hematologic cancer in the body is destroyed. The intracellular signal transduction domain of the CAR includes 4-1BB, a CD3zeta T cell activation chain and a genetically engineered 'TLE3, which can be ectopically expressed.

Provided herein is a method for user controlled gene expression. In one embodiment, provided is a method for the activation of a T cell, comprising the steps of ectopically expressing farnesoid X receptor (FXR) in said T cell; activating said FXR; and transcribing a gene that contains a farnesoid response element (FRE) in a promoter region.