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250 results about "Response element" patented technology

Response elements are short sequences of DNA within a gene promoter region that are able to bind specific transcription factors and regulate transcription of genes. Under conditions of stress, a transcription activator protein binds to the response element and stimulates transcription. If the same response element sequence is located in the control regions of different genes, then these genes will be activated by the same stimuli, thus producing a coordinated response.

In vivo biosensor apparatus and method of use

InactiveUS6673596B1Less can be administeredCost-effective administration of drugBioreactor/fermenter combinationsBiological substance pretreatmentsIn vivoGenetically engineered
Disclosed are bioluminescent bioreporter integrated circuit devices that detect selected analytes in fluids when implanted in the body of an animal. The device comprises a bioreporter that has been genetically engineered to contain a nucleic acid segment that comprises a cis-activating response element that is responsive to the selected substance operably linked to a gene encoding a bioluminescent reporter polypeptide. In preferred embodiments, the target analyte is glucose, glucagons, or insulin. Exposure of the bioreporter to the target substance causes the response element to up-regulate the nucleic acid sequence encoding the reporter polypeptide to produce a luminescent response that is detected and quantitated. In illustrative embodiments, the bioreporter device is encapsulated on an integrated circuit that is capable of detecting the emitted light, processing the resultant signal, and then remotely reporting the results. Also disclosed are controlled drug delivery systems capable of being directly or indirectly controlled by the detection device that provide drugs such as insulin to the animal in reponse to the amount of target analyte present in the body fluids.
Owner:UNIV OF TENNESSEE RES FOUND +1

Shoe with sensors, controller and active-response elements and method for use thereof

Active-response golf shoes are disclosed. The golf shoes include at least one sensor, a controller, and at least one active-response element. The sensor and controller operate to rapidly determine if a golfer is walking or swinging a golf club. Once this determination is made the controller and active-response element rapidly change the shoe's characteristics. If the controller determines that the golfer is walking, the shoe provides a soft and flexible walking platform. If the controller determines that the golfer is swinging, the shoe morphs or changes automatically to provide a stable hitting platform. The controller senses various predetermined conditions such as pressure under the ball of the user's foot to determine whether the golfer is walking or swinging. The active-response elements may be a sole adjuster, a lace adjuster, and / or an upper adjuster. Methods of determining the golfer's type of movement are also disclosed.
Owner:ACUSHNET CO

Shoe with sensors, controller and active-response elements and method for use thereof

Active-response golf shoes are disclosed. The golf shoes include a plurality of sensors, a controller, and at least one active-response element. The sensor and controller operate to rapidly determine if a golfer is walking or swinging a golf club. Once this determination is made the controller and active-response element rapidly change the shoe's characteristics. If the controller determines that the golfer is walking, the shoe provides a soft and flexible walking platform. If the controller determines that the golfer is swinging, the shoe morphs or changes automatically to provide a stable hitting platform. The controller senses various predetermined conditions such as pressure of the user's foot to determine whether the golfer is walking or swinging. The active-response element is a lateral adjuster having an upper bracket located in the laces area and a lower bracket affixed to the side of the sole, brackets supporting a plurality of electrostrictive polymer bands;
Owner:ACUSHNET CO

Diacylhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex

The present invention relates to non-steroidal ligands for use in nuclear receptor-based inducible gene expression system, and a method to modulate exogenous gene expression in which an ecdysone receptor complex comprising: a DNA binding domain; a ligand binding domain; a transactivation domain; and a ligand is contacted with a DNA construct comprising: the exogenous gene and a response element; wherein the exogenous gene is under the control of the response element and binding of the DNA binding domain to the response element in the presence of the ligand results in activation or suppression of the gene.
Owner:PRECIGEN INC

Response element

The present invention is directed to a novel Afx response element comprising the nucleotide sequence AACATGTT, said nucleotide sequence having a DNA binding site for the human fork head transkription factor Afx. The invention also relates to the use of the Afx response element in the screening for genes as diabetes drug targets and in the bioinformatic analysis of the human genome, said genes in turn being useful in other screening methods for compounds modifying the insulin receptor signaling pathway. A further aspect of the invention is a vector construct comprising the novel nucleotide sequence, a host cell transformed with said vector construct as well as the fusion protein expressed by said host cell.
Owner:BIOVITRUM AB (PUBL) +1

Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex

The present invention relates to non-steroidal ligands for use in nuclear receptor-based inducible gene expression system, and a method to modulate exogenous gene expression in which an ecdysone receptor complex comprising: a DNA binding domain; a ligand binding domain; a transactivation domain; and a ligand is contacted with a DNA construct comprising: the exogenous gene and a response element; wherein the exogenous gene is under the control of the response element and binding of the DNA binding domain to the response element in the presence of the ligand results in activation or suppression of the gene.
Owner:PRECIGEN INC

Estrogenic extracts of Morus alba and uses thereof

Extracts of various species of the Moraceae family have estrogenic properties. For example, aqueous and ethanolic extracts of Morus alba L. species possess estrogenic properties in both ERα+ and ERβ+ cells. These estrogenic effect include estrogen response element (ERE) stimulation as well as tumor necrosis factor (TNF) repression. Methods are provided for treating climacteric symptoms, breast and / or uterine cancer, and osteoporosis.
Owner:BIONOVO

Oxadiazoline ligands for modulating the expression of exogenous genes via an ecdysone receptor complex

The present invention relates to non-steroidal ligands for use in nuclear receptor-based inducible gene expression system, and a method to modulate exogenous gene expression in which an ecdysone receptor complex comprising: a DNA binding domain; a ligand binding domain; a transactivation domain; and a ligand is contacted with a DNA construct comprising: the exogenous gene and a response element; wherein the exogenous gene is under the control of the response element and binding of the DNA binding domain to the response element in the presence of the ligand results in activation or suppression of the gene.
Owner:PRECIGEN INC

Methods for modulating expression of exogenous genes in mammalian systems, and products related thereto

In accordance with the present invention, there are provided various methods for modulating the expression of an exogenous gene in a mammalian subject employing modified ecdysone receptors. Also provided are modified ecdysone receptors, as well as homomeric and heterodimeric receptors containing sane, nucleic acids encoding invention modified ecdysone receptors, modified ecdysone response elements, gene transfer vectors, recombinant cells, and transgenic animals containing nucleic acids encoding invention modified ecdysone receptor.
Owner:EVANS RONALD +2

Shoe with sensors, controller and active-response elements and method for use thereof

Active-response golf shoes are disclosed. The golf shoes include a plurality of sensors, a controller, and at least one active-response element. The sensor and controller operate to rapidly determine if a golfer is walking or swinging a golf club. Once this determination is made the controller and active-response element rapidly change the shoe's characteristics. If the controller determines that the golfer is walking, the shoe provides a soft and flexible walking platform. If the controller determines that the golfer is swinging, the shoe morphs or changes automatically to provide a stable hitting platform. The controller senses various predetermined conditions such as pressure of the user's foot to determine whether the golfer is walking or swinging. The active-response element is a lateral adjuster having an upper bracket located in the laces area and a lower bracket affixed to the side of the sole, brackets supporting a plurality of electrostrictive polymer bands.
Owner:ACUSHNET CO

Method and system for communicating within a messaging architecture using a structured data object

A method, system, and computer-program product for communicating within a messaging architecture using a structured data object are disclosed. The method includes identifying a structured data object configured to represent structured data, receiving the structured data at a messaging system, generating a message that includes the structured data, and sending the message. The structured data object is configured to be accessed by an application and the messaging system, and includes a request element and a response element. The sending includes transferring the message via the messaging system.
Owner:ORACLE INT CORP

System and method for generating a single use password based on a challenge/response protocol

A system and method which generates a single use password based on a challenge / response protocol. A box manager module executing within a security appliance identifies a public key (P) and salt value (S) associated with an administrator's smart card and generates a random nonce (N). The box manager transmits a challenge comprising the following elements: <SHA1(N), BM_ID, P[N, BM_ID], S>. Upon receiving the challenge, the administration card decrypts P[N, BM_ID] using the private key contained within the card and computes SHA1(N). The administration card then compares its computed values with the received values from the box manager. If the values match, then to the administration card returns a response comprising the following elements: HMAC_N[user, SHA1 (password, S)], where HMAC_N represents the SHA1 keyed hash message authentication check of the response elements using the nonce N as the key.
Owner:NETWORK APPLIANCE INC

Live Attenuated Influenza Virus Vaccines Comprising Microrna Response Elements

The invention is directed to novel live attenuated influenza virus (LAIV) vaccines comprising one or more microRNA (miRNA) Response Element(s) (MRE) within an influenza virus genome. The MREs useful for the present invention can be derived from any miRNA which is highly expressed in influenza-targeted cells of an animal in need of vaccination but are not expressed or are expressed at very low levels in species (e.g., embryonated chicken eggs) or cell lines used for a large-scale vaccine production. This allows efficient vaccine production but renders the vaccine virus susceptible to attenuation in the influenza-targeted cells of vaccinated animals expressing a cognate miRNA.
Owner:MT SINAI SCHOOL OF MEDICINE

Estrogenic extracts of Morus alba and uses thereof

Extracts of various species of the Moraceae family have estrogenic properties. For example, aqueous and ethanolic extracts of Morus alba L. species possess estrogenic properties in both ERα+ and ERβ+ cells. These estrogenic effect include estrogen response element (ERE) stimulation as well as tumor necrosis factor (TNF) repression. Methods are provided for treating climacteric symptoms, breast and / or uterine cancer, and osteoporosis.
Owner:BIONOVO

Treatment of diseases associated with the egr-1 enhancer element

Compounds and methods are provided for treating patients suffering from health condition associated with an expression state of a gene such as fertility disorders, cancer, proliferative diseases, vascular diseases, wounds requiring therapeutic intervention, inflammation, and pulmonary disorders by administering to said patient a compound capable of modulating egr-1 and / or an egr-1 response element consensus sequence thereby altering the expression state of said gene. Also described are new methods for screening compounds to identify effectors of egr-1 and / or egr-1 consensus sequence elements and methods for treating patients by administering such effectors to modulate egr-1 and / or egr-1 consensus sequences to thereby modify expression of genes associated therewith to in turn treat diseases or other physiological conditions associated with such gene expression.
Owner:RESVERLOGIX

Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes

Disclosed is a method using an alkane response element (ARE) from, e.g., Acinetobacter spp. to (i) identify and clone hydrocarbon biosynthesis genes, (ii) identify and clone hydrocarbon transporter genes (iii) identify and clone hydrocarbon response genes. Screening cells were developed that expressed a transcriptional activator, e.g., alkR, and included a reporter gene, e.g., GFP operatively linked to an ARE promoter, e.g., the alkM promoter. The cells were transformed with libraries from organisms capable of hydrocarbon biosynthesis. Transformed cells that expressed the reporter gene harbored library-derived genes involved in one or more of the above-mentioned processes; and these genes were isolated from the cells using standard molecular biology techniques. Additional systems were designed wherein screening cells also expressed a gene identified in the original screen, e.g., an additional hydrocarbon pathway gene, e.g., an enhancer.
Owner:LS9 INC +1

ESTROGENIC EXTRACTS OF Pueraria lobata Willd. Ohwi of the Leguminosae Family AND USES THEREOF

Extracts of various species of the Leguminosae family have estrogenic properties. For example, aqueous and ethanolic extracts of Pueraria lobata Willd. Ohwi of the Leguminosae family species possess estrogenic properties in both ERα+ and ERβ+ cells. These estrogenic effects include estrogen response element (ERE) stimulation as well as tumor necrosis factor (TNF) repression. Methods are provided for treating climacteric symptoms, breast and / or uterine cancer, and osteoporosis.
Owner:BIONOVO

Transgenic plants containing molecular decoys that alter protein content therein

InactiveUS20060057723A1Reduce the amount requiredLowering carcinogenic potentialFungiBacteriaNicotiana tabacumDecoy
The present application describes isolated nucleic acids that contain a Nic gene product responsive element, and the use thereof in methods of producing transgenic tobacco plants having reduced levels of nicotine and / or TSNA therein, as well as other plants or host cells that contain altered levels of a protein of interest therein due to inclusion of a cis-acting decoy element therein.
Owner:CONKLING MARK +1

Messaging architecture configured to use an execution-enabled element to initiate an operation in an application

A method, system, and computer-program product for communicating within a messaging architecture using a structured data object are disclosed. The method includes receiving an execution-enabled object and receiving a selection of the execution-enabled element. Such an execution-enabled object is configured to be included in a message sent by a messaging system. The execution-enabled object includes a response element and an execution-enabled element. The response element and the execution-enabled element are associated with one another. The execution-enabled element is configured to post an event to the messaging system upon the execution-enabled element being activated. The selection of the execution-enabled element activates the execution-enabled element.
Owner:ORACLE INT CORP

Adenovirus vectors containing cell status-specific response elements and methods of use thereof

The present invention provides adenoviral vectors comprising cell status-specific transcriptional regulatory elements which confer cell status-specific transcriptional regulation on an adenoviral gene. A “cell status” is generally a reversible physiological and / or environmental state. The invention further provides compositions and host cells comprising the vectors, as well as methods of using the vectors.
Owner:CG ONCOLOGY INC

Estrogenic extracts of ligustrum lucidum ait. of the oleaceae family and uses thereof

Extracts of various species of the Moraceae family have estrogenic properties. For example, aqueous and ethanolic extracts of Ligustrum lucidum Ait. of the Oleaceae Family possess estrogenic properties in both ERα+ and ERβ+ cells. These estrogenic effects include estrogen response element (ERE) stimulation as well as tumor necrosis factor (TNF) repression. Methods are provided for treating climacteric symptoms, breast and / or uterine cancer, and osteoporosis.
Owner:BIONOVO

Tamoxifen and 4-hydroxytamoxifen-activated system for regulated production of proteins in eukaryotic cells

Novel tamoxifen inducible and ICI 182,780 repressible expression systems comprising mutant estrogen receptors and mutant estrogen response element are disclosed. Such systems have a wide variety of applications, including gene therapy and in vivo and in vitro expression, as well as their use in transgenic animals.
Owner:THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS

Protection against oxidative stress and inflammation by a cytoprotective response element

This invention is in the area of regulatory DNA sequences and their methods of use. Specifically, DNA sequences found in the regulatory region of cytoprotective genes are described that are termed cytoprotective response elements. DNA constructs also are provided that include cytoprotective response elements operably linked to heterologous protein coding sequence, as well as cells and non-human organisms that include the cytoprotective response elements optionally operably linked to a heterologous protein coding sequence, and a method for screening for a compound that increases mRNA or protein regulated by a cytoprotective response element. It has been discovered that the cytoprotective response elements mediate the coordinate activation of certain genes that protect cells from damaging effects of oxidative stress, and that they do so, for example during conditions of hemodynamic shear stress.
Owner:SALUTRIA PHARMACEUTICALS LLC

Method for enriching and separating endogenous transcription factors and compounds thereof and concatenated transcription factor response elements special for method

The invention discloses a method for enriching and separating endogenous transcription factors and compounds thereof and concatenated transcription factor response elements special for the method. The method includes of designing multi-copy double-stranded DNA (deoxyribonucleic acid) binding elements according to the characteristic that transcription factors are bound with sequence-specific DNA elements, adding double-stranded DNA arms labeled with biotin to two ends of each DNA binding element by the aid of molecular cloning technology to form 'DNA baits', coupling the biotinylated DNA baits which are purified onto magnetic beads enveloped by streptavidin when in separation and purification of the endogenous transcription factors and compounds thereof, incubating the magnetic beads together with the prepared nucleoprotein, and finally separating and purifying the endogenous transcription factors and the compounds thereof from the nucleoprotein. Further, after the endogenous transcription factors and the compounds thereof are separated and purified, protein identification and function analysis of the transcription factors can be performed subsequently.
Owner:INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +1

Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex

The present invention relates to non-steroidal ligands for use in nuclear receptor-based inducible gene expression system, and a method to modulate exogenous gene expression in which an ecdysone receptor complex comprising: a DNA binding domain; a ligand binding domain; a transactivation domain; and a ligand is contacted with a DNA construct comprising: the exogenous gene and a response element; wherein the exogenous gene is under the control of the response element and binding of the DNA binding domain to the response element in the presence of the ligand results in activation or suppression of the gene.
Owner:RHEOGENE INC DE

Marker-free gene deletion attenuated mutant strain of Edwardsiella tarda wild strain as well as relevant preparations and application thereof

The invention relates to a marker-free gene deletion attenuated mutant strain of an Edwardsiella tarda wild strain. The marker-free gene deletion attenuated mutant strain is an attenuated live vaccine of an Edwardsiella tarda virulent strain, which deletes the chorismic acid synthase gene aroC of the Edwardsiella tarda virulent strain, three types of secretion system response element genes of eseB, escA, eseC and eseD and an endogenous plasmid, preferably, the Edwardsiella tarda virulent strain is an Edwardsiella tarda virulent strain EIB202 with the preservation number of CCTCC No:M208068; the endogenous plasmid is a plasmid of pEIB202; and the marker-free gene deletion attenuated mutant strain of the Edwardsiella tarda virulent strain is an attenuated strain WED with the preservation number of CCTCC No:M2010278. The invention also provides relevant preparations and application of the marker-free gene deletion attenuated mutant strain. The attenuated mutant strain or relevant preparations eliminate the potential environment and safety risk of products existing in the traditional attenuated live vaccines generally and is a safe, effective and economic vaccine aiming at Edwardsiella tarda diseases of cultured fishes.
Owner:EAST CHINA UNIV OF SCI & TECH

Safety lock

A safety lock for a closing-off device which can be opened, has an actuating unit with an actuator which is guided in a displaceable manner, and has a locking unit into which the actuator, with the closing-off device closed, can be pushed and can be locked via an electromagnetically actuable, pivotable catch, by way of interengaging engagement surfaces, the actuator has a trigger element and the locking unit has a response element which, with the actuator pushed in, reacts to the trigger element by emitting an electric signal which triggers the locking action, in the case of which the catch can be pivoted about an axis extending parallel to the movement direction of the actuator, and the engagement surfaces of the catch and of the actuator run perpendicularly to the movement direction.
Owner:K A SCHMERSAL

Compositions and methods for the modification of physiological responses in plants

A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected protein that modifies sensitivity to ethylene in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, modulates expression of the selected protein and selectively modulates ethylene sensitivity in the plant cell. This modulation in the expression of the protein is controlled by the timing, the concentration and the duration of the application of the inducing composition. Transgenic plant cells, tissues, organs and entire plants are provided, which in the presence of the inducing composition control ethylene sensitivity. Ethylene sensitivity and / or ethylene production in such transgenic plants and tissues may be controlled for purposes of manipulating ripening, flower senescence and other ethylene sensitive functions of the plant.
Owner:AGROFRESH
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