84 results about "Mannan" patented technology

The invention belongs to the technical field of biological medicines, and relates to application of mannatide in preparation of a medicine for treating alcoholic gastric ulcer. The mannatide disclosed by the invention can be used for remarkably relieving gastric tissue hemorrhagic lesion and ulcer conditions of alcoholic gastric ulcer for the first time, and the mechanism comprises the following steps: reducing active oxygen generated by alcohol toxicity, and improving oxidative stress of a body by improving antioxidant enzyme activity of the gastric tissue; the inflammatory response of the organism is regulated by regulating and controlling an Akt / NF-kB pathway; a Bax-related apoptosis pathway is inhibited to protect gastric mucosa cells from alcohol-induced apoptosis, so that the integrity of gastric tissue mucosa is protected; the intestinal flora disorder caused by alcohol intake is regulated and controlled, and the acute alcoholic gastric ulcer is prevented by improving the intestinal flora composition of the alcoholic gastric ulcer. The application range of the mannatide is widened, and more solutions are provided for prevention or treatment of acute alcoholic gastric ulcer.

Disclosed is a cancer vaccine that contains dendritic cells. The dendritic cells are prepared by: culturing immature dendritic cells to maturation; co-culturing the matured dendritic cells with attenuated cancer cells, Mannan – BAM (Mannan, a yeast-derived polysaccharide, and BAM, a Biocompatible Anchor for Cell Membrane), and TLR (Toll like receptor) agonists; and optionally sorting the dendritic cells from the co-cultured attenuated cancer cells.

The invention discloses a composite particle with a prebiotic function and colon-targeted co-delivery performance as well as a preparation method and application thereof, and belongs to the technical field of functional foods and intestinal micro-ecological regulators. According to the composite particle, the curcumin and the resveratrol are encapsulated by the peanut protein isolate to form a nano core, the outer layer of the nano core is a composite shell layer composed of the carboxymethyl konjac glucomannan and the prebiotics, a stable core-shell structure is formed, and the composite particle is small in particle size, good in dispersity and stability and good in stability. The stability of a protein-based delivery system in a gastrointestinal tract environment can be obviously improved; the release rate of the composite particles in simulated gastric juice and intestinal juice is low, and the composite particles can be specifically degraded and release active ingredients in simulated colon fluid containing beta-mannase, so that colon-targeted co-delivery can be realized. The composite particle disclosed by the invention is simple in preparation process and stable in function, can be applied to the fields of functional foods, health foods, formula foods for special medical purposes and the like, realizes dual purposes of active component delivery and intestinal microenvironment regulation, and has a wide application prospect.

The invention relates to the technical field of probiotic preparations, in particular to a composite prebiotic embedded lactobacillus rhamnosus microcapsule as well as a preparation method and application thereof. The preparation method of the composite prebiotics embedded lactobacillus rhamnosus microcapsule comprises the following steps: (1) stirring and mixing isomaltooligosacharide and galactomannan in water, then adding sodium alginate, and stirring and mixing to obtain a mixed solution; (2) mixing the mixed solution with a bacterium suspension of lactobacillus rhamnosus to obtain a compound bacterium solution; and (3) injecting the compound bacteria liquid into the curing liquid to obtain gel beads, and then standing to obtain the gel beads. According to the microcapsule provided by the invention, three encapsulating materials are adopted to achieve a synergistic effect, so that the encapsulating efficiency of the microcapsule can be effectively improved, and meanwhile, the structural stability of the microcapsule is improved. Experiments prove that the microcapsule can effectively improve the survival rate of the probiotics in gastrointestinal tracts, more accurately control release of the probiotics and provide technical support for development of probiotic functional products.

The invention relates to the field of functional additives for daily chemicals, and particularly discloses hydrolyzed procyanidine freeze-dried powder and a preparation method thereof. The hydrolyzed procyanidine freeze-dried powder is prepared by freeze-drying and comprises the following raw materials in parts by weight: 0.1-0.2 part of hydrolyzed procyanidine, 25-35 parts of carnosine, 10-20 parts of a stabilizer, 0.1-0.3 part of acetyl hexapeptide-8 and 45-55 parts of water. The stabilizer is selected from at least one of mannan, trehalose, mannitol, cane sugar, betaine, whey protein, PVP (Polyvinyl Pyrrolidone), PEG (Polyethylene Glycol), glycine, histidine, leucine, sodium alginate, beta-cyclodextrin, maltodextrin and chitosan. The hydrolyzed procyanidine freeze-dried powder has good storage stability, can keep activity for a long time, is still stable after being dissolved in water for a long time, and has less activity loss.

The utility model discloses disc type separating equipment, which relates to the technical field of mannatide preparation equipment and comprises a disc type separating machine main body, the disc separator main body is arranged on the buffer mounting seat; wherein the buffer mounting seat comprises a mounting top plate, a mounting bottom plate and a plurality of damping shock absorbers, the mounting bottom plate is arranged on the mounting structure, the mounting top plate is arranged above the mounting bottom plate, and the damping shock absorbers are arranged between the mounting top plate and the mounting bottom plate and detachably connected with the mounting top plate and the mounting bottom plate; the disc separator main body is arranged on the mounting top plate and is detachably connected with the mounting top plate, so that the problem that the axis of a transmission rotating shaft in the conventional disc separator is easy to deviate, crack and even break after being used for a long time is solved.

The invention discloses a method for preparing ganoderan rich in a mannogalactan structure from a Chryseobacterium bacterium GS-7 and Ganoderma lucidum YJ0724 and application of the ganoderan rich in the mannogalactan structure, and relates to the technical field of microbial fermention.The method comprises the following steps that a Chryseobacterium bacterium GS-7 seed solution is prepared, and a Ganoderma lucidum YJ0724 seed solution is prepared; the method comprises the following steps: a, simultaneously inoculating a seed solution of a Chryseobacterium bacterium GS-7 and a seed solution of Ganoderma lucidum YJ0724 into a fermentation medium for co-culture to obtain a fermentation solution containing Ganoderma lucidum polysaccharide rich in a mannan galactan structure; or b, filtering and degerming the fermentation liquor of the Chryseobacterium bacterium GS-7, and inoculating the fermentation liquor and the ganoderma lucidum YJ0724 seed solution into the culture medium for co-culture to obtain the fermentation liquor containing the ganoderma lucidum polysaccharide rich in the mannogalactan structure. The obtained ganoderma lucidum polysaccharide rich in the mannogalactan structure can improve the immunomodulatory activity.

The application discloses a process for improving the quality of palm kernel meal feed by enzyme and bacteria synergistic fermentation, and belongs to the field of feed biotechnology.The process is characterized by the following steps: crushing and screening palm kernel meal, mixing the palm kernel meal with 20% bran and 4% ammonium sulfate as dry matter to obtain a basic substrate;compounding bacillus subtilis, aspergillus niger and saccharomyces cerevisiae at a ratio of 1:1:1 of viable bacterial number, adding 0.67% mannanase and 0.3% xylanase to prepare a bacteria-enzyme mixed solution, inoculating the bacteria-enzyme mixed solution at a 10% inoculation amount, adjusting the moisture content to 50% and the pH to 6.4, and then sealing and storing the bacteria-enzyme mixed solution for use after 4 days of fermentation at 33.5 DEG C; and the bacteria-enzyme mixed solution can be stored for a long time.The process can efficiently degrade crude fiber and anti-nutritional factors, improve the protein content and the digestibility, is stable and easy to mass-produce, can replace part of corn and soybean meal in the daily ration of ruminants, and realizes high-value utilization of palm kernel meal.

The application belongs to the technical field of microcapsules, and particularly relates to a preparation method of a composite probiotic capsule, which comprises the following steps: uniformly mixing probiotic bacterial liquid, fructooligosaccharide, tea polyphenol, grape polyphenol, mannan oligosaccharide, isomaltitol and an emulsifier to obtain a core material; uniformly mixing glycerol and distilled water, heating to 60-80 DEG C, sequentially adding modified gelatin, hydroxypropyl starch and chitosan, stirring for 10-30 min, sterilizing, and cooling to room temperature to obtain a capsule material; mixing the core material and the capsule material, performing high-pressure homogenization treatment, and then performing spray drying to obtain the composite probiotic capsule; and the modified gelatin is prepared by acylation reaction of succinic acid derivatives and gelatin. The composite probiotic capsule provided by the application has excellent storage stability, antioxidant property, anti-gastric juice erosion capacity, and also has good enteric solubility and bioavailability.

This invention belongs to the field of biological breeding technology, specifically involving SlCSLA2 Application of genes in improving the storage and transportability of tomato fruit. This invention addresses the industry problem of severe storage and transport losses in tomatoes due to excessive softening after harvest. For the first time, it utilizes CRISPR / Cas9 gene editing technology to knock out the cell wall hemicellulose (mannan) synthase gene. SlCSLA2 This invention has led to the creation of a tomato mutant with significantly enhanced fruit firmness. The firmness of the entire fruit during the red-ripe stage is 35%–42% higher than that of the wild type, and the firmness can be maintained for about a week after harvest, effectively delaying the softening process and significantly improving storage and transportability. This invention provides new gene targets and technical approaches for breeding tomato varieties with improved storage and transportability.

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to mannase derived from space mutagenesis yeast strains as well as a preparation method, a product and application of the mannase. The amino acid sequence of the mannase is as shown in SEQ ID NO.2, and the nucleotide sequence of the mannase is as shown in SEQ ID NO.3. The mutant retains 70% of enzyme activity at a high temperature (85 DEG C for 3 min, and only 40% of original enzyme remains at 85 DEG C for 3 min) and has an acid pH value (the stability is improved from 3% to 25% after treatment for 180 min at pH 3.0), and meanwhile, the mutant has the low-temperature efficient catalytic characteristics that the optimum pH value is 7.0, the optimum temperature is 40 DEG C, and 30% of enzyme activity is kept at 20 DEG C, and has remarkable application potential in the fields of feed, paper-making industry and the like.

The preparation method comprises the following steps: (1) compounding hermetia illucens larvae, whole tenebrio molitor and euphausia superba according to a mass ratio of (3-5): (2-4): 1, carrying out enzymolysis, and reacting with a yeast cell wall extract to form a glycopeptide compound; (2) coating the glycopeptide compound with a mannan oligosaccharide and chitosan solution to form microcapsules, and controlling the release rate of the microcapsules in simulated gastric juice to be lower than 30% and the release rate of the microcapsules in simulated intestinal juice to be higher than 80%; (3) co-fermenting the microcapsules, clostridium butyricum and pediococcus acidilactici, and adsorbing fermentation liquor on the porous starch; (4) performing dry mixing on the functional compound, the fermentation compound and the base material, and performing low-temperature puffing; and (5) sequentially spraying a vitamin D3 grease layer, a pectin-whey protein protective layer and a solution containing TGF-beta2 on the surfaces of the particles. Through cooperation of multiple processes, the immune function and intestinal health of pets are remarkably improved, and the product is good in stability and palatability.

The present disclosure provides synthetic compositions useful as inhibitors of mannan-binding lectin-associated serine protease-2 (MASP-2), including compositions that selectively inhibit MASP-2 over thrombin, as well as methods for their production and use.

The utility model relates to the technical field of mannose oligomer, and provides a positioning device for mannose oligomer filling, which comprises a conveyor, a controller and an L-shaped plate fixedly mounted on the rear side of the conveyor, and two electric push rods are oppositely and fixedly mounted on the inner top wall of the L-shaped plate. During use, the conveyor is started to convey a filling bottle, when the infrared sensor detects the filling bottle, information is transmitted to the controller, the controller controls the conveyor to be closed, the electric push rod is started, the electric push rod pushes the discharging pipe on the strip-shaped plate to move downwards, the discharging pipe extends into the filling bottle, and the filling bottle is discharged. Meanwhile, mannan-oligosaccharide is pumped out through a pump body in the feeding mechanism, the amount of the conveyed mannan-oligosaccharide is detected through a solid flow meter, when the amount reaches a set range, information is transmitted to a controller, the pump body in the feeding mechanism is closed through the controller, and the mannan-oligosaccharide is conveyed out through the solid flow meter; therefore, the purposes of automatic filling and quantitative filling of the mannose oligomer are achieved.

The invention belongs to the field of biological fermentation feeds, and discloses a preparation method and application of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation, and particularly relates to a preparation method of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation by taking Sophora alopecuroides straw as a raw material, adopting a complex microbial inoculant composed of lactobacillus plantarum, saccharomyces cerevisiae, bacillus subtilis and aspergillus niger, cooperating with cellulase and beta-mannase, and performing multi-bacterial enzyme synergistic fermentation. The compound is prepared through a two-stage fermentation process of aerobic enzyme production and anaerobic detoxification. The invention provides a preparation method and application of a Sophora alopecuroides feed based on multi-bacterial enzyme synergistic fermentation, toxic alkaloids in Sophora alopecuroides can be efficiently degraded, the crude protein content can be significantly increased, and fibers can be degraded, and after the prepared fermented feed replaces corn in daily ration of mutton sheep, the daily gain of the mutton sheep can be significantly increased, the feed-to-weight ratio can be reduced, and the production cost can be reduced. The flavor and the nutritional value of the mutton are improved, high-valued and safe utilization of toxic agricultural wastes is realized, and a new scheme is provided for green breeding and high-quality animal product production.

The invention relates to the technical field of daily chemical products and cosmetics, in particular to a scalp repair composition containing epidermis repair polysaccharide and saccharomycetes lysate. The skin repairing composition is prepared from the following raw materials in percentage by mass: 1 to 5 percent of skin repairing polysaccharide; 1%-5% of a yeast lysate; 1%-10% of PEG-5 (Polyethylene Glycol) glycerate triisostearate; and the balance of cosmetically acceptable matrix. The polysaccharide is mannan which is obtained by fermenting Paenibacillus sp.1538, and the weight-average molecular weight of the mannan is 6.15 * 10 < 6 > Da. The specific ultra-high molecular weight polysaccharide is compounded with the saccharomyces cerevisiae extract, and the low-irritation PEG-5 glycerate triisostearate is introduced, so that the compatibility problem that the high molecular weight polysaccharide is easy to flocculate is effectively solved. The composition can form a film immediately, deeply resist inflammation, relieve and repair a physical barrier of scalp, has an excellent synergistic effect, and solves the problem of scalp chemical stimulation injury caused by a traditional surfactant from the source.

The invention relates to the technical field of feed production, and particularly provides a compound enzyme and application thereof in enzymolysis of palm meal. The compound enzyme is specially designed according to the nutritional characteristics of the palm meal, non-starch polysaccharide components such as protein, cellulose and mannan in the palm meal can be effectively subjected to enzymolysis, the anti-nutrition effect of non-starch polysaccharide is reduced, the content of soluble oligosaccharide, small molecular protein and the like is increased, and the feeding value of the palm meal is improved.

The invention discloses a preparation method for improving the stability of soybean protein isolate-konjac mannan emulsion, and belongs to the technical field of application of vegetable protein structures. The method comprises the following steps: (1) fully dissolving a soybean protein isolate solution with a certain concentration, and fully hydrating; (2) adding a certain amount of konjac mannan, and reacting at high temperature; and (3) adding six different sodium salt ions into the soybean protein isolate-konjac mannan compound. And (4) respectively mixing the samples in the steps (1), (2) and (3) with sunflower seed oil, and shearing at a high speed to obtain the emulsion. Six sodium salt ions are introduced, the emulsion particle size of the soybean protein isolate-konjac mannan compound is reduced through the Hough-Merster effect, the stability of the emulsion is improved, and under the freezing condition, the emulsion also shows higher freeze injury resistance and emulsion cracking resistance. The method has a certain reference value for the food industry.

A method for obtaining highly sulfated nanoparticles of cellulose or sulfated nanoparticles of mannan, or a combination thereof, from endosperms, comprising acquiring a ground flour of an endosperm of a seed, nut, or pit, wherein the ground flour contains polysaccharides, and wherein the polysaccharides contain either cellulose or mannan, or both cellulose and mannan; pretreating the ground flour to condition the polysaccharides for hydrolysis and functionalization thereof; preparing a reagent for hydrolysis and functionalization of the polysaccharides, wherein the reagent comprises a solution of glycerol, water, and sulfuric acid at predetermined concentrations; adding the reagent to the pretreated polysaccharides to form a suspension, wherein adding the reagent to the suspension converts the cellulose or mannan or both into nanoparticles that are sulfated by the reagent; separating the sulfated nanoparticles from the suspension; washing the nanoparticles; and creating a stable colloidal suspension of the sulfated nanoparticles. The sulfated nanoparticles are used in pharmaceutical, cosmetic, wound healing, and skin treatment formulations.

The invention belongs to the field of biomass energy utilization, and particularly relates to a method for preparing high-value dehydrated sugar through rapid pyrolysis based on extremely dilute acid high-temperature modification. The method comprises the following steps: by taking palm plant seed waste as a raw material, carrying out boiling-washing modification at a temperature close to a boiling point by using an acid aqueous solution with extremely dilute concentration, preferably further carrying out cold plasma discharge modification treatment, and then carrying out rapid pyrolysis under an anaerobic condition at 350-450 DEG C, and condensing the obtained pyrolysis steam to obtain a liquid product rich in levomannan (LM, 1, 6-anhydro-beta-D-mannopyranose). According to the method, ash can be effectively removed, the reaction activity of the raw materials is improved, high-purity enrichment of LM is promoted, and a technical approach is provided for high-value utilization of palm plant seed waste.

The application belongs to the field of genetic engineering and enzyme engineering, and particularly relates to a mannanase from a space mutagenized yeast strain, a preparation method, a product and an application thereof. The amino acid sequence of the mannanase is shown as SEQ ID NO. 2, and the nucleotide sequence thereof is shown as SEQ ID NO. 3. The mutant has outstanding performances at high temperature (70% enzyme activity is retained at 85 DEG C for 3 min, while 40% enzyme activity is retained for the original enzyme at 85 DEG C for 3 min) and acidic pH (the stability is increased from 3% to 25% after treatment at pH 3.0 for 180 min), and has low-temperature high-efficiency catalytic characteristics of optimal pH 7.0, optimal temperature 40 DEG C and 30% enzyme activity at 20 DEG C, and has significant application potential in the fields of feed and papermaking industry.

The invention discloses an application of an endo-beta-mannase coding gene GhMAN7 and an SNP (Single Nucleotide Polymorphism) associated with the endo-beta-mannase coding gene GhMAN7 in regulation and control of plant seed vigor, and belongs to the technical field of molecular breeding. According to the invention, through whole genome association analysis (GWAS) and expression quantitative trait locus (eQTL) co-localization, the SNP (A / G) located at the chromosome 16685321 of the cotton D05 is identified as a key effect site for regulating and controlling the seed activity. The site is remarkably associated with the expression level of an endo-beta-mannase gene GhMAN7 (the cDNA sequence is as shown in SEQ ID NO.1), and directly causes extremely remarkable difference between the germination rate and the early root length of cotton seeds and other characters. The invention provides an accurate target spot and an efficient solution for molecular marker-assisted selection of high seed vigor cotton varieties.

The invention discloses a dendrobium officinale polysaccharide, a derivative and a preparation method, and belongs to the field of polysaccharides, the preparation method of the dendrobium officinale polysaccharide comprises the following steps: step 1, obtaining a crude polysaccharide precipitate; dissolving the crude polysaccharide precipitate with purified water, and sequentially carrying out deproteinization, decoloration, desalination and small molecule removal treatment; removing salt and small molecular impurities, concentrating the purified liquid, and performing freeze-drying or spray-drying to obtain parent polysaccharide; 2, the obtained parent polysaccharide is dissolved in a buffer solution, endo-beta-1, 4-mannanase is added, directional enzymolysis oligomerization of a beta-1, 4-mannan skeleton is achieved through a reaction, enzyme activity is terminated after the reaction is finished, insoluble substances are removed after cooling, and enzymatic hydrolysate is obtained and used for subsequent grading; and step 3, removing suspended impurities from the obtained enzymatic hydrolysate, sequentially carrying out series separation by using ultrafiltration membranes or nanofiltration membranes with molecular weight cutoff of 1kDa, 3kDa and 10kDa, and carrying out post-treatment to obtain the dendrobium officinale oligosaccharide product.

The invention provides a preparation method of extracellular vesicles carrying mycobacterium tuberculosis specific antigens, and belongs to the technical field of extracellular vesicles. THP-1 mononuclear cells are induced to be differentiated into macrophages through phorbol ester, a mycobacterium tuberculosis H37Rv standard strain is treated through ultrasonication and needle blowing and beating to prepare a monodisperse bacterium solution, and the monodisperse bacterium solution is added into the mycobacterium tuberculosis specific antigens to prepare the extracellular vesicles carrying the mycobacterium tuberculosis specific antigens. Infecting according to the infection complex value of 5, monitoring the real-time infection rate every 12 hours in the culture process, dynamically adjusting the initial bacterial liquid concentration according to the value to maintain the infection rate in the range of 45-55%, purifying the extracellular vesicles through a differential centrifugation method, detecting the particle size and concentration of the vesicles and the content of lipoarabinomannan, and determining the content of lipoarabinomannan in the extracellular vesicles. Qualified vesicle products are divided into three concentration grades according to the antigen content, and dimethyl sulfoxide is added for cryopreservation, so that the technical problem of poor antigen loading stability among batches of extracellular vesicle products carrying mycobacterium tuberculosis specific antigens is solved.

The invention relates to a bacteriostatic feed and a preparation method and application thereof, the bacteriostatic feed comprises a basic ration and yeast essence, the content of beta-glucan in the yeast essence is 25-50%, and the relative molecular weight of more than 99% of beta-glucan is 3.0 * 10 < 5 >-1.0 * 10 < 6 >; the content of mannan in the yeast essence is greater than or equal to 20%, and the relative molecular weight of mannan of 99% or above is 8.0 * 10 < 5 >-2.0 * 10 < 6 >. The yeast essence involved in the feed disclosed by the invention is added into the feed as a single substance, the recommended dosage is stable, and the yeast essence shows a relatively good resistance replacement effect in in-vitro inhibition zone simulation and in-vivo test verification of bred animals; a set of efficient and feasible antibiotic substitution scheme can be provided for the animal breeding industry, meanwhile, the defects of antibiotic drug resistance and residues and poor effect and poor stability of current antibiotic substitution products are overcome, and the future prospect is considerable.

The invention discloses immunity-enhancing nutritional freeze-dried powder for cats and a preparation method of the immunity-enhancing nutritional freeze-dried powder, and relates to the technical field of freeze-drying. The preparation method comprises the following steps: taking chicken breast granules, tenebrio molitor powder, hermetia illucens larva powder, chicory root paste, puree of kudzuvine root, puree of broccoli, puree of blueberry, puree of carrot, puree of pumpkin, puree of cranberry, puree of brown algae, puree of chlorella, astragalus membranaceus concentrated solution, bovine colostrum powder, zinc methionine chelate, iron lysine chelate, lactoferrin, mannan oligosaccharide, embedded bifidobacterium lactis powder, docosahexaenoic acid and linoleic acid; uniformly stirring yeast beta-glucan, a rosemary extract, selenium-enriched yeast, coenzyme Q10 and natural vitamin E, and performing high-pressure homogenization to obtain slurry; forming the slurry through a brace, an extrusion device or a scraping die to obtain formed slurry; performing ultrasonic treatment on the formed slurry; gradient boosting and constant pressure are carried out; freezing and thawing; performing pre-freezing; performing vacuum freeze drying; after the drying is finished, slowly introducing dry and sterile nitrogen; and screening, carrying out quality inspection, sub-packaging and sealing to obtain the immunity-enhancing nutrient freeze-dried powder for cats.

The application belongs to the technical field of veterinary medicine preparation, and particularly relates to a mannose peptide with dosage form conversion and a preparation method and application thereof. The mannose peptide with dosage form conversion comprises the following raw materials: mannose peptide: 5-20 parts by weight; carrier, the carrier is composed of sugar alcohol and lactose, sugar alcohol: 20-60 parts by weight; lactose: the weight ratio of the lactose to the sugar alcohol is (0.75-2):1; wetting dispersant: 0.5-3 parts by weight; carrier stabilizer: 2-10 parts by weight; the carrier stabilizer is soluble polyvinylpyrrolidone or copolymer. The mannose peptide with dosage form conversion has cold water instant dissolving capacity, and can be directly used as an instant drinking water agent. Meanwhile, without any pretreatment, the mannose peptide with dosage form conversion can be directly used for fluidized bed coating of a pill core to prepare colon-targeting pellets, so that the process is simplified and the drug administration mode is flexibly converted.

The utility model relates to the technical field of detection, and discloses an accurate quantitative sampler for mannan detection, which comprises a shell. At the moment, a handle is rotated to drive a threaded rod to rotate, one end of the threaded rod is fixedly connected with a sliding block, the sliding block rotates in a supporting block through an inner groove, a rubber ring extrudes mannan liquid in a shell along the inner wall of the shell, the mannan liquid is extruded, and therefore the first rubber ring is extruded; when the first rubber ring moves downwards, the spring is compressed, and the mannan liquid flows out through the surface of the first rubber ring, and when the threaded rod stops rotating, the spring enables the first rubber ring to be attached to the fixing column again through the elastic force, the mannan liquid is prevented from flowing out, the taking amount can be accurately controlled, manual operation errors can be reduced by accurately controlling the taking amount, and the production efficiency is improved. It is ensured that results obtained in each experiment have high consistency, meanwhile, mannan liquid is protected from being polluted by other substances in the shell, and detection data are more accurate.