69 results about "Gastric Tissue" patented technology

The invention discloses an establishment method and application of a mouse gastric cancer orthotopic transplantation model, and the establishment method comprises the following steps: injecting a mouse gastric adenocarcinoma cell line MKC1 (Mus musculus) into a mouse gastric tissue, and feeding to obtain the mouse gastric cancer orthotopic transplantation model. Compared with the prior art, the mouse gastric cancer in-situ model established in the invention has the advantages of strong repeatability in the construction process and good passage stability, especially can form tumor subcutaneously in a C57BL / 6 mouse with a sound immune system, has a therapeutic response to immunotherapeutic drugs such as anti-PD-1 monoclonal antibody, and is suitable for research on gastric cancer immune microenvironment and drug resistance mechanism of immunotherapeutic drugs.

Monoclonal antibodies of molecular markers (KRT7, KLK10 and LAMC2) for specifically marking very-early-stage cells of gastric cancer are prepared, and are used in the preparation of a kit for determining the very-early-stage onset risk of gastric cancer or other digestive tract tumors on the basis of gastric tissue or blood. The results show that: 1) the overall accuracy rate of predicting the progression risk of low-grade dysplasia reaches 86%, and the AUC value reaches 0.87; 2) the accuracy rate is closely related to the progression time of low-grade dysplasia, wherein the accuracy rate is increased to 95% 6 months before the onset of gastric cancer, and the window for the early diagnosis of gastric cancer can be moved forward by an average of 10 months; and 3) the accuracy rate of the marker in diagnosing gastric cancer is over 97%.

The invention discloses a hydrogel for gastric perforation repair, which belongs to the field of bioadhesives. The technical scheme is as follows: a hydrogel for gastric perforation repair is generated by chemical reaction of compound A and compound B; compound A is a polyethylene glycol derivative A with a terminal active group α; the terminal active group α is succinimidyl succinate; compound B is a polyethylene glycol derivative B with a terminal active group β; the terminal active group β is an amino group; when compound A reacts with compound B, the terminal active group α and the terminal active group β chemically react to form an amide bond. The beneficial effects of the invention are as follows: the hydrogel for gastric perforation repair provided by the invention has a fast gelation speed, can be gelled in situ, is easy to use, has strong adhesion to gastric tissue, has a low swelling rate in simulated gastric fluid and PBS solution, has a strong ability to resist simulated gastric fluid corrosion, has good compression resistance and anti-peristalsis performance, has good biocompatibility, and can be safely degraded.

The invention relates to the technical field of medicines, in particular to a pharmaceutical composition for improving acute alcoholic gastric mucosal lesion and application. The invention discloses the new medical application of the lucid ganoderma, ginseng and kudzuvine root formula for the first time, and experiments and clinical verifications prove that the formula can increase the activity of key enzymes in stomach and liver tissues of a mouse with acute alcoholic gastric mucosal injury, reduce the content of a proinflammatory factor TNF-alpha in the stomach tissues, increase the generation of an anti-inflammatory factor IL-10 in the stomach tissues, relieve the inflammatory injury of ethanol to gastric mucosa, improve the curative effect of alcohol on the gastric mucosa and improve the curative effect of alcohol on the gastric mucosa. The levels of protective factors EGF, SS and PGE-2 in serum are increased, the defense mechanism of gastric mucosa is enhanced, and the repairing capacity of the gastric mucosa is improved. The discovery has great influence on the research and treatment of acute alcoholic gastric mucosal injury diseases, and has clinical practical value.

The invention belongs to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for treating chronic atrophic gastritis and a preparation method and application thereof. According to the technical scheme, the traditional Chinese medicine composition is characterized by being prepared from, by weight, 10-50 parts of radix codonopsis, 5-20 parts of roasted rhizoma atractylodis macrocephalae, 5-10 parts of rhizoma pinellinae praeparata, 2-8 parts of rhizoma coptidis, 8-40 parts of poria cocos, 5-15 parts of radix salviae miltiorrhizae, 3-6 parts of rhizoma zingiberis, 5-15 parts of rhizoma cyperi, 1-20 parts of radix actinidiae chinensis and 1-5 parts of radix glycyrrhizae preparata. The traditional Chinese medicine composition provided by the invention can improve the pathological morphology of stomach tissues, increase the expression of PG I and the ratio of PG I to PG II, reduce the level of inflammatory factors and improve the atrophy degree of gastric mucosa, and is used for treating stomachache, distention and fullness, anorexia, gastric noisy and other symptoms caused by spleen and stomach qi deficiency, qi stagnation and blood stasis and damp-toxin interjunction of patients with chronic atrophic gastritis.

The invention provides a wound sealing and repairing hydrogel patch and a preparation method thereof. The hydrogel patch is provided with an independent adhesion layer and an adhesion layer and anti-adhesion layer combination. The adhesive layer is prepared from gelatin, N-acryloylated amino acid, acrylic acid-N-succinimide ester, 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone and water through ultraviolet light crosslinking; the anti-adhesion layer is prepared from methacryloyl ethyl sulphobetaine, polyethylene glycol diacrylate, 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone and water through ultraviolet light crosslinking. The hydrogel patch can be used for sealing and repairing wounds of various tissues (such as skin tissues, liver tissues and stomach tissues), the healing time is shortened, the healing effect is improved, the healing time of a skin incision can be shortened from 14 days to 10 days, the hydrogel patch is superior to other treatment modes according to macroscopic photos and related slices, and the hydrogel patch has a good application prospect. And no-injury healing can be realized.

The embodiment of the invention relates to the technical field of medical detection, and provides a stomach tissue pathology risk assessment method and device, electronic equipment and a medium, and the method comprises the following steps: obtaining target Raman spectrum detection data of a stomach tissue sample of a current patient; inputting the detection data into the stomach tissue pathology risk assessment model to obtain an output result, and further determining a stomach tissue pathology risk assessment result of the current patient; the method comprises the following steps: acquiring Raman spectrum detection data corresponding to a plurality of stomach tissue samples by the stomach tissue pathology risk assessment model; dividing the detection data into a training set and a verification set; the initial model is trained based on the training set, and then the verification set is evaluated; and when the effectiveness of the assessment result meets a preset condition, obtaining a stomach tissue pathology risk assessment model. Therefore, the Raman spectrum and the artificial intelligence technology are combined for evaluating the pathological risk of the stomach tissue, rapid and accurate detection of the cancer, non-cancer and precancerous pathological stages of the stomach tissue sample is realized, and a diagnosis reference is provided for doctors.

The invention belongs to the technical field of biological medicines, and relates to application of mannatide in preparation of a medicine for treating alcoholic gastric ulcer. The mannatide disclosed by the invention can be used for remarkably relieving gastric tissue hemorrhagic lesion and ulcer conditions of alcoholic gastric ulcer for the first time, and the mechanism comprises the following steps: reducing active oxygen generated by alcohol toxicity, and improving oxidative stress of a body by improving antioxidant enzyme activity of the gastric tissue; the inflammatory response of the organism is regulated by regulating and controlling an Akt / NF-kB pathway; a Bax-related apoptosis pathway is inhibited to protect gastric mucosa cells from alcohol-induced apoptosis, so that the integrity of gastric tissue mucosa is protected; the intestinal flora disorder caused by alcohol intake is regulated and controlled, and the acute alcoholic gastric ulcer is prevented by improving the intestinal flora composition of the alcoholic gastric ulcer. The application range of the mannatide is widened, and more solutions are provided for prevention or treatment of acute alcoholic gastric ulcer.

The invention belongs to the technical field of medicines, and particularly relates to application of fructus cinnamoii in preparation of a medicine for treating gastric ulcer. The invention discloses the application of the fructus cinnamoii oil in preparation of the gastric ulcer medicine for the first time, specifically, the fructus cinnamoii oil obviously improves the conditions of rat gastric mucosa bleeding, erosion, hyperemia, edema and the like and also obviously reduces the ulcer index of rat gastric tissues, and in addition, the fructus cinnamoii oil can be used for preparing the gastric ulcer medicine by reducing TNF-alpha, IL-6 and MDA. The contents of SOD and GSH are increased to improve the inflammatory reaction and oxidative stress of stomach tissues, so that the effect of protecting the stomach tissues of rats is achieved.

The invention discloses glycyrrhiza polysaccharide for preventing and treating acute stomach injury and a preparation method and application thereof. The molecular weight of the glycyrrhiza polysaccharide is 60 kDa, the percentage content of protein is smaller than or equal to 5%, and the mass percentage content is larger than or equal to 60% according to glucose; the preparation method comprises the following steps: carrying out ethanol reflux degreasing on liquorice, carrying out water extraction and alcohol precipitation, removing protein by adopting Sevag, dialyzing by using a 8-13 kDa dialysis bag, carrying out rotary concentration, and freeze-drying to obtain the glycyrrhiza polysaccharide. Animal experiments show that the glycyrrhiza polysaccharide has a protective effect on acute alcoholic stomach injury, the content of MDA and MPO in animal stomach tissues can be reduced, and the stomach injury is relieved by reducing lipid oxidation and neutrophil infiltration level; the content of gastric tissue pepsase and VEGF (vascular endothelial growth factor) can be increased, and the gastric mucosa can be protected in the aspects of maintaining the stomach environment, promoting blood vessel healing and reducing the injury time and degree; and the medicine also has a pathological repair effect on acute stomach injury.

The invention relates to an image segmentation network method based on dual-path cross-latitude attention, and belongs to the technical field of medical image processing and deep learning. The method comprises the following steps: an input layer performs size adjustment and standardization processing on a stomach tissue pathological image; the encoder adopts a dual-path down-sampling architecture, combines a dual-path feature interaction mechanism, extracts multi-scale features through depth separable convolution and fuses the multi-scale features; the shallow jump connection layer extracts features based on a cross-latitude feature fusion mechanism by dividing cross dimension and standard space dimension branches and fuses the features to generate jump connection features; the decoder adopts an up-sampling architecture and is combined with a composite pooling attention enhancement mechanism to extract local features and generate decoding features; and the output layer outputs a lesion region segmentation result through single-channel convolution and an activation function. The accuracy and stability of lesion area segmentation of the stomach tissue pathological image are effectively improved, and reliable image analysis support is provided for clinical pathological diagnosis.

An implantable device for the treatment of acid reflux disease has a body including an outer surface adapted to rest against a patient's fundus wall. The device, when implanted in the patient, fills a volume in the patient's abdomen in the cardia area and above the cardia of the patient, when standing. The body prevents the cardia from moving through the diaphragm opening into the thorax, thereby preventing reflux by keeping the abdominal pressure supporting the cardia sphincter muscle. The device may be affixed to stomach tissue. The device may also be invaginated or otherwise indirectly secured in position.

The application provides application of a Baoji oral liquid composition in preparation of a functional dyspepsia medicine, and relates to the technical field of medicines. The application is based on the Baoji oral liquid, and it is found that the Baoji oral liquid has a remarkable effect on treating functional dyspepsia, especially cisplatin-induced functional dyspepsia. Specifically, the Baoji oral liquid can rapidly restore body weight, significantly improve body weight gain, steadily promote food intake, significantly improve gastric emptying rate and intestinal propulsion rate, protect the gastric mucosa layer, reduce inflammatory cell infiltration, repair the mucosa layer and muscle layer, improve the expression of serum gastrin and motilin, and improve the expression level of IL-4 in the gastric tissue and reduce the expression level of IL-1beta. The Baoji oral liquid has remarkable efficacy when applied in preparation of a functional dyspepsia medicine.

The invention relates to a proteolysis product with gastric mucosa repair activity and application thereof. The protein zymolyte with gastric mucosa repair activity is prepared by the following method: firstly, carrying out primary enzymolysis on mycoprotein by using a first enzyme to obtain a primary enzymolysis solution, and then carrying out secondary enzymolysis on the primary enzymolysis solution by using a second enzyme to obtain a secondary enzymolysis solution, the secondary enzymatic hydrolysate is a solution in which the protein hydrolysate with gastric mucosa repair activity is dispersed; the first enzyme comprises aspergillus oryzae protease; the second enzyme comprises aspergillus niger lipase. The proteolysis product provided by the invention has the effects of increasing the antioxidant level of gastric tissues, improving the defense factor level of gastric mucosa and reducing the level of related inflammatory factors in serum, can play a role in protecting the gastric mucosa, has gastric mucosa repairing activity, and is safe and easily available in raw materials, simple in preparation method and easy for batch production.

The present application relates to the technical field of medicine, in particular to a kind of drug composition for improving acute alcoholic gastric mucosal injury and application.The present application discloses the new medical use of ganoherbae decoction for the first time, and the composition can increase the activity of key enzyme in the stomach and liver tissue of acute alcoholic gastric mucosal injury mice, reduce the content of pro-inflammatory factor TNF-α in gastric tissue, increase the production of anti-inflammatory factor IL-10 in gastric tissue, reduce the inflammatory damage of ethanol to gastric mucosa, increase the level of protective factor EGF, SS and PGE-2 in serum, enhance the defense mechanism of gastric mucosa, and improve the repair ability of gastric mucosa.The discovery will have a significant impact on the research and treatment of acute alcoholic gastric mucosal injury disease, and has clinical practical value.

The helicobacter pylori bispecific antibody comprises a first antibody, a connector and a second antibody, the first antibody (VHH) is specifically combined with a helicobacter pylori urease B subunit (UreB) antigen, the second antibody (ScFv) is specifically combined with a helicobacter pylori vacuolar toxin protein (VacA) antigen, the first antibody (VHH) is connected with the second antibody (ScFv) through the connector, and the second antibody (ScFv) is connected with the second antibody (ScFv) through the connector. The whole or part of the connector is a heavy chain constant region derived from humanized lgG1 or lgG2 or lgG3 or lgG4; the bispecific antibody can significantly reduce adhesion of helicobacter pylori in the stomach, and reduce infiltration of inflammatory cells and damage of stomach tissues; the helicobacter pylori bispecific antibody can quickly and obviously reduce the infection ability of helicobacter pylori, and does not generate other harmful effects on stomach tissues and cells.

The present disclosure provides methods and formulations for treating a patient suffering from one or more of chronic inflammatory injury, metaplasia, dysplasia or cancer of esophageal tissue and gastric tissue, which method comprises administering to the patient an agent that selectively kills or inhibits the proliferation or differentiation of pathogenic Barrett's Esophagus stem cells (BESCs) or Gastric Intestinal Metaplasia stem cells (GIMSCs) relative to normal regenerative esophageal stem cells or gastric stem cells in the tissue in which the BESCs or GIMSCs are found.

The composition provided by the invention takes bear gall wood as a monarch drug and takes earthworm, bletilla striata, bighead atractylodes rhizome, Chinese yam and white paeony root as ministerial drugs, the earthworm and bletilla striata jointly play the effects of promoting blood circulation to remove meridian obstruction, dispersing blood stasis, removing stasis, astringing to stop bleeding and repairing injury, and the bighead atractylodes rhizome and Chinese yam are used as ministerial drugs and are used for strengthening spleen and stomach, replenishing qi and blood, providing fundamental power for the whole prescription and supporting healthy qi; liver softening, spasm relieving and pain relieving can be achieved; meanwhile, the traditional Chinese medicine composition has the effects of nourishing blood and astringing yin, can prevent other medicines in the formula from being too warm and dry or consuming yin blood, and forms dynamic and static combination compatibility with the minister medicine earthworm for promoting blood circulation to remove meridian obstruction; hairyvein agrimony, herba violae, vernonia graveolens, cuttlebone, mangnolia officinalis, semen raphani and endothelium corneum gigeriae A large number of pathological experiments prove that the traditional Chinese medicine composition can reduce stomach tissue inflammation, oxidative stress and activate apoptosis signal channels by acting on MAPK, PI3K-Akt and HIF-1 signals, so that the effect of treating chronic atrophic gastritis is achieved.

The invention relates to hybrid nano-clay and a preparation method and application thereof, the hybrid nano-clay comprises hybrid nano-clay particles with adhesive power, the hybrid nano-clay particles comprise nano-clay with the surface modified with a metal polyphenol network, and the metal polyphenol network is formed by self-assembly of Zn < 2 + > and tannic acid; the mass percent of the metal polyphenol network in the hybridized nano-clay particles is not less than 33%. The hybridized nano-clay particles provided by the invention have the capabilities of resisting oxidation, resisting bacteria and stopping bleeding; the gel can be adhered to stomach tissues, and free radicals in the body can be efficiently removed; the secretion of gastric mucosa protection factors can be stimulated, the complete form of the gastric mucosa is maintained, the damaged gastric mucosa is comprehensively protected and repaired, and the stomach health level is remarkably improved.

There is provided a gastric tissue tumour model that is three-dimensional comprising from 0.8 to 1.2 w / v % alginate, from 1.7 to 2.3 w / v % gelatin and gastric decellularized extracellular matrix (dECM). The gastric tissue model is for example an esophageal cancer tumour model which advantageously replicates the tumour microenvironment for esophageal cancer and can be used to perform a three-dimensional culture of esophageal tumour cells or metastatic cells that often metastasize into the esophagus.

The invention relates to a medical apparatus (101) for carrying out an endoscopic gastric remodelling procedure comprising a sizing tube (2) which has an inner lumen (21), at least one vacuum port (22) for applying suction to stomach tissue (19) and forming at least two tissue bulges (191, 192) of the stomach tissue (19), and at least one tissue receiving opening (23, 24) extending in a longitudinal direction (L) of the sizing tube (2) for receiving the tissue bulges (191, 192) based on the applied suction. The medical apparatus (101) has a puncturing connection unit (3), characterized in that the puncturing connection unit (3) is adapted for connecting the two tissue bulges (191, 192) with each other by puncturing the tissue bulges (191, 192), and an expansion unit (11) for spreading the stomach which has a collapsed configuration (111) and an expanded configuration (112), wherein the expansion unit (11) in the expanded configuration is configured for engaging the greater curvature (196) of the stomach and advancing the sizing tube towards the lesser curvature (195) of the stomach.

An implantable device for the treatment of acid reflux disease has a body including an outer surface adapted to rest against a patient's fundus wall. The device, when implanted in a patient, fills a volume in the patient's abdomen in the cardia area and above the cardia of the patient, when standing. The body prevents the cardia from moving through the diaphragm opening into the thorax, thereby preventing reflux by keeping the abdominal pressure supporting the cardia sphincter muscle. The device may be affixed to stomach tissue. The device may also be invaginated or otherwise indirectly secured in position.

Disclosed are methods of inducing formation of a gastric cells and / or a gastric tissue, such as in the form of a gastric organoid. The formation of gastric cells and / or tissue may be carried out by the activating and / or inhibiting of one or more signaling pathways within a precursor cell. Also disclosed are methods for using the disclosed gastric cells, gastric tissues, and / or gastric organoids derived from precursor cells.

This application discloses the use of Panax notoginseng polysaccharide in the preparation of drugs for the prevention or treatment of alcoholic gastric ulcers. The research in this application shows that Panax notoginseng polysaccharide can reduce the area of ​​gastric ulcers, alleviate mucosal edema and congestion; the drug reduces the levels of GAS, IL-6, IL-10, and TNF-α and MPO activity in gastric tissue, and can be used in drugs and health products for the prevention or treatment of alcoholic gastric ulcers.

The invention discloses application of an Lnc-PSMB8-AS1 gene in preparation of a medicine for treating gastric cancer and a diagnostic kit, and belongs to the field of biomedical treatment. Statistic analysis is carried out on the expression quantity of the Lnc-PMSB8-AS1 gene in gastric cancer patient tissues and normal gastric tissues in a TCGA database, and it is found that the expression quantity of the Lnc-PMSB8-AS1 gene in the gastric cancer tissues is obviously higher than that in the normal tissues. The method comprises the following steps of: transfecting Lnc-PMSB8-AS1-siRNA, then verifying at the lncRNA level by using a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology, and displaying that after transfecting Lnc-PMSB8-AS1-siRNA, the mRNA level expression of MGC-803 cell related migration and proliferation genes MMP7, MMP14, cyclinB1 and cyclinE1 is obviously reduced. Meanwhile, in combination with phenotypic experimental results, the Lnc-PMSB8-AS1 is prompted to have good application in preparation of diagnostic kits and targeted therapeutic drugs for gastric cancer.

The invention belongs to the technical field of biological medicine, discloses nanocapsule-loaded in-situ gel for treating H. pylori infection and reversing intestinal metaplasia of gastric tissue and application of the nanocapsule-loaded in-situ gel, and particularly discloses nanocapsule-loaded in-situ gel which comprises a nanocapsule and pH-responsive gel, the nanocapsule is dispersed in the gel, and the pH-responsive gel is a pH-responsive gel. Comprising an active drug, a macromolecular substance for coating the active drug and a structure stabilizer. According to the nanocapsule-loaded in-situ gel provided by the invention, the nanocapsule-loaded in-situ gel is directionally released on one side by responding to the change of the pH value of an H.pylori infected part, and the nanocapsule responds to lipase secreted by H.pylori to accurately release an active drug, so that efficient sterilization is realized, and damage caused by infection is relieved. Meanwhile, the gel can also effectively reverse gastrointestinal metaplasia caused by H.pylori infection, further reduces the risk of gastric cancer, and provides a new thought for gastric cancer prevention.

The invention relates to the technical field of biology, in particular to a polypeptide for inhibiting helicobacter pylori. The polypeptide can significantly inhibit the growth of helicobacter pylori and reduce the urease activity of the helicobacter pylori, not only can effectively reduce the colonization number of the helicobacter pylori in the stomach, but also can inhibit the generation of proinflammatory cytokine interleukin 1 beta and tumor necrosis factor alpha, promote the secretion of anti-inflammatory cytokine interleukin 10, inhibit the growth of the helicobacter pylori and inhibit the growth of the helicobacter pylori. The preparation has huge potential in preventing and treating damage of helicobacter pylori to gastric tissues, and has practical application and popularization values.

To provide an embeddable device for curing a backflow of a gastric acid or fatness which has a main body having an outer face constituted so as to abut on a stomach bottom wall of a patient, and fills a capacity in an abdominal part of the patient in a patient cardia region and above the cardia of the patient when the patient takes an erecting posture when the device is embedded into a body of the patient.SOLUTION: A main body prevents a cardia from intruding into a thoracic cavity through a diaphragm opening part, and thereby prevents a backflow by maintaining abdominal pressure for supporting a cardia sphincter. A device can be fixed to a gastric tissue. Also, the device can be infiltrated into a proper position, or indirectly fixed to a proper position by the other method. The device has a size of at least 125 mm3, and an external periphery of at least 15 mm.SELECTED DRAWING: Figure 1A

The present invention relates to the field of medical biotechnology, and particularly to a method for evaluating the ability of gastric tissue to produce IL-1β. This evaluation method uses the transcriptional level of Aquaporin (AQP9) to evaluate the ability to generate IL-1β in the gastric tissue microenvironment. According to the experimental results, the potential of IL-1β produced in gastric tissue can be accurately evaluated. This evaluation method shortens the detection time and is of great significance for early evaluation of the degree of inflammation under pathophysiological conditions.

The invention discloses a manufacturing method of a magnetic response millimeter-scale adhesion mobile robot for intragastric targeted drug delivery and pathological detection and the adhesion mobile robot. The method comprises the following steps: manufacturing a magnetic sheet with a simple harmonic magnetization curve; manufacturing a flexible frame, wherein a plurality of mounting holes for accommodating magnetic sheets are formed in the flexible frame; each magnetic sheet with a simple harmonic magnetization curve is mounted to a flexible frame, thereby forming a magnetic response millimeter-scale adhesion mobile robot for intragastric targeted drug delivery and pathological detection. According to the adhesion mobile robot manufactured through the method, the thin liquid film is transmitted on the wet and slippery surface through wave type movement of the magnetic response sheet-shaped soft material, so that crawling propelling is achieved by means of counter-acting force generated by the liquid film, the robot can stably crawl on the wet and low-friction stomach tissue surface by means of the adhesion moving characteristic, and the robot has a good application prospect. Good environmental adaptability is shown.