102 results about "Early Gastric Cancer" patented technology

The invention discloses an enzyme linked immunosorbent assay kit for combined diagnosis of the gastrosis or evaluation of gastric cancer risks and a preparation method thereof. The kit comprises a micropore plate coated with an antibody against a pepsin antigen I or an antibody against a pepsin antigen II, an enzyme labeled antibody, a color-developing agent, a stop solution and a concentrated cleaning solution, wherein the pepsin antigen I or the pepsin antigen II is a natural protein obtained from extraction of human gastric mucosa tissue. The kit disclosed by the invention adopts a mouse immunized with pepsinogen I and pepsinogen II which are separated from human gastric mucosa to prepare immunogen of a monoclonal antibody, the used standard sample also adopts the pepsin antigen I or the pepsin antigen II separated from the human gastric mucosa, thereby the defects caused by adopting different structures of animal pepsinogen and human pepsinogen are filled. The kit can be used for accurately diagnosing the gastrosis or early gastric cancer and has the advantages of high sensitivity, strong specificity, good accuracy and the like.

The invention relates to a gastric early cancer histological image classification system based on a deep neural network, and the system comprises a WSI pathological section obtaining module which is used for obtaining a gastric early cancer pathological section image; an image preprocessing module which is used for preprocessing the early gastric cancer pathological section image; a data set division module which is used for dividing the data set after image preprocessing into a training set and a verification set, a training set which is used for model training, and the verification set is used for verifying a model effect; a feature extraction module which is used for extracting picture features of the training set or the verification set based on a GoogleNet double-branch network structure and outputting feature vectors of corresponding pictures; and a classifier training module which is used for training based on the neural network model to obtain a gastric early cancer classification model.

The invention relates to a kit for a serum / plasma lncRNA marker related to stomach cancer. The serum / plasma lncRNA marker is combination of MALAT1 and HOTAIR, a primer is a primer for the MALAT1 and the HOTAIR, and the upstream primer sequence and the downstream primer sequence of the MALAT1 are SEQ No.1 and SEQ No.2; the upstream primer and the downstream primer of the HOTAIR are SEQ No.3 and SEQ No.4. By means of the kit, the defect that a kit only adopting one serum / plasma lncRNA as the tumor marker is low in sensitivity and specificity, so that the fault diagnosis rate and the rate of early gastric cancer diagnosis are greatly raised is overcome; by means of the kit, the sensitivity and specificity of disease diagnosis are enhanced, successful development of the lncRNA biological marker is beneficial to auxiliary diagnosis of stomach cancer and provides reference for research of biological markers of other diseases, lncRNA chip detection is adopted to obtain a serum / plasma lncRNA expression profile for disease specificity and abnormity expression, a qRT-PCR method is applied to conduct single verification, and dye method is adopted to conduct verification.

The invention belongs to the technical field of chip detection and particularly discloses an antibody chip kit for detecting early gastric cancer. The antibody chip kit is characterized in that various gastric cancer marker related antibodies are fixed onto a solid-phase carrier, and the specific antibodies are screened from 35 routine gastric cancer marker related antibodies. The antibody chip kit can detect a plurality of gastric cancer related antigen proteins at the same time, and the detection effect of a combined antibody chip is better than that of single tumor markers.

The invention discloses a screening method and application of a plasma microRNA marker for identifying a gastric cancer. According to the screening method, cel-miR-39 serving as an external reference and miR-16 serving as an internal reference are used together, and data of to-be-tested microRNA are normalized, so as to improve the reliability of qRT-PCR data. On the basis of the method, plasma microRNA markers, namely miR-19b, miR-671-5p and miR-101, are screened, wherein the miR-19b and the miR-671-5p are used for distinguishing normal people from the patients with gastric cancer at different stages and different differentiated degrees, and can be used as the markers for gastric cancer screening; and especially the miR-671-5p is used for specifically distinguishing the patient with early gastric cancer from normal people. Therefore, the plasma microRNA markers can be used as characteristic markers for early gastric cancer diagnosis. The miR-101 is used for distinguishing the patients with gastric cancer before and after an operation, and can be used as a prognosis marker of the gastric cancer.

The invention discloses a compound fingerprint atlas model used in early-stage gastric cancer diagnosis / early warning, and an establishing method thereof. According to the invention, the concentration of a trace amount of volatile organic compound in a gastric cancer cell metabolic product is separated and detected by using a gas chromatograph-mass spectrometer, and the atlas model is drawn. A mass volume concentration ratio of 4-isopropoxy butanol to nonanal to 4-butoxyl n-butanol is represented by that: 4-isopropoxy butanol [gastric cancer cell] / [normal gastric mucosa cell] is no higher than 0.31, nonanal [gastric cancer cell] / [normal gastric mucosa cell] is no higher than 0.36, and 4-butoxyl n-butanol [gastric cancer cell] / [normal gastric mucosa cell] is no higher than 0.40. The gastric cancer cell metabolic product has characteristic peaks of 3-octanone and 2-butanone. Volatile organics in a cell metabolic product requiring detection are compared with the fingerprint atlas model provided by the invention, such that the fingerprint atlas model can be used in early-stage gastric cancer diagnosis and early warning.

The present invention relates to a novel use of the SDC2 (NM_002998, syndecan 2) gene as a biomarker of a gastric polyp and gastric cancer-specific methylation, and more particularly, to a use for diagnosing a gastric polyp and gastric cancer in an early stage by measuring the methylation level using the SDC2 gene as a biomarker. The present invention has the effect of providing a method which can provide information for the diagnosis of gastric cancer by detecting the methylation of a CpG island of a gastric polyp and a gastric cancer-specific marker gene. Also, using the method for detecting methylation and a composition, kit, and nucleic acid chip for diagnosis according to the present invention, gastric cancer can be diagnosed at an initial transformation stage, and thus, early diagnosis can be achieved. Therefore, the present invention is useful as it is possible to diagnose gastric cancer more accurately and rapidly than conventional methods.

A set of biomarkers indicative of early gastric cancer and method of diagnosing gastric cancer at an early stage by directing the these biomarkers in a blood sample. Detection of over-expression of one or more protein biomarks in the group consisting albumin, T-kininogen I, α-2-HS glycoprotein, α-1-antitrypsin, afamin and γ-actin and / or detection of under-expression of one or more protein biomarks in the group consisting stress 70 protein, apolipoprotein A-I, apolipoprotein A-IV, transthyretin and murinoglobulin is indicative of the presence of gastric cancer.

The invention discloses a gene marker for screening early gastric cancer. The gene marker comprises a peripheral blood gastric cancer characteristic gene sequence shown as SEQ ID NO.1-SEQ ID NO.6. The invention also discloses application of the gene marker in preparation of products for screening early gastric cancer. The gene marker disclosed by the invention is used for screening early gastric cancer and is high in sensitivity and specificity; and moreover, because the peripheral blood which can be easily collected in clinical application is taken as a detection sample, the detection is convenient, the gene marker is applicable to screening and early diagnosis of a large population for gastric cancer, and the application prospects are wide.

The invention relates to a methylated biomarker or a combination thereof for detecting gastric cancer lymph node metastasis and non-metastasis and application of the methylated biomarker. The methylated biomarker is selected from at least one of chr12: 42873140, chr8: 81789921, chr8: 81811441 and chr8: 16884489. According to the invention, the appropriate DNA methylation biomarker is screened and used for detecting gastric cancer lymph node metastasis and non-metastasis, and the DNA methylation biomarker can achieve the purpose of accurate prediction. The novel DNA methylation marker developed by the invention can be used for identifying whether the lymph node metastasis of the early gastric cancer occurs or not, can be used for assisting clinical accurate diagnosis and guiding treatment, and also can be used for technical research in laboratories.

The invention discloses an auxiliary diagnosis system for early gastric cancer under an electronic staining endoscope based on deep learning, and the system comprises an image obtaining module which obtains a stomach image collected in real time in an examination process of the electronic staining endoscope; an early gastric cancer recognition module used for recognizing a focal zone and marking the focus area based on a pre-constructed early gastric cancer recognition model; and a logic judgment module used for judging whether the identified focal zone belongs to the same focal zone or not when the identified focal zone is overlapped. By means of a common electronic staining means, the invention provides the method for identifying the gastric early cancer based on an electronic staining endoscopic image, and the real-time performance, accuracy and practicability of detection are greatly improved.

The early stage stomach cancer detecting method with serum is to utilize fingerprint comprising ten proteins of charge / mass ratio 4141 Da, 4474 Da, 3940 Da, 4633 Da, 4792 Da, 3957 Da, 4976 Da, 5348 Da, 4096 Da and 5915 Da separately in detection. The detecting method includes the following specific steps: preparing serum, collecting mass spectrum data, and biological informatics analysis of the collected data. The present invention provides new concept and method for the early stage diagnosis of stomach cancer and lays foundation of new tumor marker. The method of the present invention is reasonable and feasible, and has combined biological informatics method and analysis method with specificity and sensitivity over 84 % each and greatly raised stomach cancer detecting rate.

The invention relates to a kit for the identification of gastric cancer and / or gastric polyps and application thereof. The kit comprises a primer pair, wherein the primer pair is used for detecting the methylation level of a biological marker gene or corresponding segments in a biological sample of a tester; the primer pair is used for performing PCR (polymerase chain reaction) amplification reaction by using the biological marker gene or segments thereof treated by bisulfite as a template; the biological marker gene is selected from one or multiple of CDH1, DAPK, PAX5, RASSF1A, Reprimo, RNF180, RUNX3, SDC2, Septin9 and TCF4. The kit has the advantage that by jointly detecting the methylation level of the biological marker gene or corresponding segments, the sensitivity and specificity indetection of the gastric cancer and / or gastric polyps is improved, so that the correctness and reliability of the detection results are guaranteed, and a quick, reliable and accurate novel path is provided for the predicting, diagnosis and evaluation on the gastric cancer and / or gastric polyps.

The invention relates to a molecular marker hsa-circ-0000705 for a gastric cancer and an application of the molecular marker hsa-circ-0000705. A nucleotide sequence is as shown in SEQ ID NO.1. The molecular marker has excellent gastric cancer tissue specificity and gastric cancer relevance; quantitative determination can be achieved through an RT-PCR method; a novel molecular marker is provided for discovery and detection of an early gastric cancer; and the molecular marker has certain significance in further improvement of the current gastric cancer diagnosis and treatment level.

The invention discloses a method for constructing a tumor xenograft model in mice immunized with gastric cancer based on an organ-like method, and an application thereof. The invention relates to a medical tumor animal model, based on an organ-like method. For the first time, the present invention employs a quasi-organ technique, that is, using a microcarrier as substrate. The composite human gastric cancer cell line SGC-7901 / MKN-45 is transplanted subcutaneously into C57BL / 6 mice to establish a tumor model of human gastric cancer with normal immune function. The tumor model constructed by this method has good tumorigenicity, and the tumorigenicity rate is up to 75%. The HE staining and the immunohistochemistry of the transplanted tumor accord with the characteristics of human gastric cancer. Compared with the existing immunodeficient mice transplanted tumor model, the model can better study and further clarify the mechanism of tumor occurrence and development in the normal immune system, and also provides a more valuable new animal model for the research and development of anticancer drugs. The invention is applied to the tumor model field.

The invention belongs to the field of medical equipment and particularly relates to a medical tool, namely a detachable gastroscope cannula with an inflatable airbag, which performs gastroscopic examination and surgery according to the modern medicine. The detachable gastroscope cannula comprises an inner pipe and an outer pipe, wherein the length of the outer pipe is smaller than that of the inner pipe; the front end and the rear end of the inner pipe extend out of the outer pipe; the middle part of the inner pipe protrudes, the outer diameter of the middle protruding part of the inner pipe is greater than those of other pipe sections of the inner pipe, meanwhile, the outer diameter of the middle protruding part of the inner pipe is greater than the inner diameter of the outer pipe, and the outer diameters of the other parts except for the middle protruding part of the inner pipe are smaller than the inner diameter of the outer pipe; the middle protruding part of the inner pipe is sleeved with an inflatable rubber ring; the inflatable rubber ring is fixedly connected with the inner pipe; and the middle protruding part of the inner pipe and the inflatable rubber ring are tightly nested with the outer pipe. According to the detachable gastroscope cannula, the detection rate of an early gastric cancer is improved, the operation efficiency is improved and the postoperative complications are reduced.

The invention discloses a mass spectrum detection method of serum polypeptide. The method comprises the following steps: the magnetic bead supported matrix method is adopted to capture the gastric cancer peptidome in serum, a magnetic separator is used to separate magnetic beads and the sample without centrifuging the sample; and then the mass spectrum method is adopted to analyse the polypeptide spectrum. The method of the invention can be used to detect the serum samples of normal persons and patients with gastric cancer, and the detection result can be used as an important detection basis for the diagnosis of early gastric cancer. The detection method of the invention is accurate, convenient and fast.

The invention discloses a culture method of 3D tumor organs for anti-tumor drug sensitivity test of gastric cancer to solve the problem of low success rate of modeling of existing tumor organs. The method comprises the following specific steps: Step 1, preparation before an experiment; Step 2, tissue sampling; Step 3, tissue enzymatic separation; Step 4, PDO tumor organ modeling; and Step 5, PDO culture. The design of the method is reasonable. By adding gastrin during the culture process, the modeling success rate and cell viability of gastric cancer organs are promoted. It is found through comparison that when the gastrin-added medium is used to culture gastric cancer PDO, the formed PDO mass increases significantly, the cell viability is also increased, and the modeled tumor formation rate is increased significantly to 80%. It shows that the use effect is good and the method can provide a basis for the test of tumor drug sensitivity, screening of anti-tumor drugs for patients and clinical medication.

Volatile organic compounds emitted from gastric cancer cell metabolite is separated and detected using HS-SPME / GC-MS. A volatile compounds fingerprint atlas-spectrum model are disclosed for early gastric cancer diagnosis / warning Volatiles 3-octanone and 2-butanone as indicators of gastric cancer cells which are not contained in the headspace of gastric mucosal cells GES-1. Meanwhile, the ratio of mass to volume to concentration of 4-isopropoxy alcohol, nonanoic acid, and 4-butoxy n-butanol is as follows: 4-isopropoxy alcohol [gastric cancer cells] / [normal gastric mucosa cells]≦0.31; nonanoic acid [gastric cancer cells] / [normal gastric mucosa cells]≦0.36; and 4-butoxy n-butanol [gastric cancer cells] / [normal gastric mucosa cells]≦0.40. The volatile organic compound in cell metabolite to be detected is compared with the fingerprint atlas-spectrum model, so as to implement screening and warning of the early gastric cancer.

The invention discloses an early gastric cancer screening system and method. The system comprises a questionnaire analysis module, a gastric function quadruple analysis module, a historical analytical result query module, a user permission management module and a login password changing module. Through the system, it is possible for rural common doctors to carry out professional screening and evaluation of gastric cancer in association with questionnaire, and screening is fast, simple and low in cost. It is recommended that a patient under high questionnaire risk to take a blood test and an examination for four gastric functions, scoring is made according to examination results, and the gastric cancer risk level is then comprehensively evaluated in connection with the score of the questionnaire; through the method, it is possible to effectively and quickly screen patients under high gastric cancer risk, it is possible to effectively increase the utilization rate of medical resources based on reduced medical burden of people, and the system and method are significant to the increase in diagnostic and cure rate of early gastric cancer and the decrease in disability rate and lethality rate.

The invention discloses an early gastric cancer image recognition method based on evolutionary neural network model compression, relates to the technical field of computer application, and solves theproblems of parameter redundancy in an existing convolutional neural network model and poor real-time performance caused by large calculated amount consumption of the neural network model in an earlygastric cancer recognition process. The method comprises the following steps: collecting an early gastric cancer image data set; training a neural network model; constructing a binary encoding mode toencode parameters in the neural network model; compressing the trained neural network model by using an evolutionary algorithm; and finely adjusting the compressed neural network model, and identifying an early gastric cancer lesion area on a newly input gastroscopy image. According to the invention, the neural network model for detection can be compressed according to the collected gastroscopy image data, so that the efficiency of the neural network model in an early gastric cancer detection task is improved, and the deep neural network method has good real-time performance in the aspect ofearly gastric cancer detection.

The present invention discloses the nine biomarkers including HIF1A, FAM84B, CRIP2, GSN, RPL15, DLG1, MAT2A, PGBD2 and ID3 are respectively selected according to their specific and unique expression profile in the gastric cancer cells or gastric cancer tissue. Therefore, the nine biomarkers are related to diagnose gastric cancer, such as detecting early gastric cancer, staging gastric cancer, predicting prognosis of gastric cancer and diagnosing lymph node metastasis. By analyzing the expression value of at least one biomarker of a sample from a subject, the subject can be precisely diagnose the risk about gastric cancer.

The invention discloses a set of markers used for early gastric cancer diagnosis. The markers are composed of INHBA and SPP1. The invention also discloses application of the INHBA and the SPP1 in preparing a kit for early gastric cancer diagnosis and a kit for predicting lymphatic metastasis status of a gastric cancer patient. The expression quantities of the INHBA and SPP1 are detected by adopting a semi-quantitative fluorescence real-time enzyme-linked reaction method, and compared with traditional pathological cytology detection, the detection of molecular markers has the advantages of small required sample size, specificity, sensitivity, accuracy, high repeatability, automation and the like. The molecular markers lay an ideal clinical application basis for the early gastric cancer diagnosis and the prediction of the lymphatic metastasis.

The invention relates to a medicine for oral administration for treating carbuncle, gastritis, gastric ulcer and early gastric cancer. The medicine for oral administration comprises, by weight, from 1 to 2 parts of bamboo skin, from 0 to 2.5 parts of cortex lycii radicis, from 1.5 to 2.5 parts of Chinese honeylocust leaves, from 1 to 3 parts of dandelion, from 20 to 60 parts of soybeans and from 0.6 to 2 parts of edible calcined gypsum. The invention further provides a method for preparing the medicine for oral administration. The medicine for oral administration has effects of replenishing and activating blood, expelling noxious substances, diminishing inflammation, relieving swelling and pain, promoting blood circulation to remove blood stasis, eliminating necrotic tissues, promoting granulation, healing sore and the like, has the outstanding advantages of capability of becoming effective fast, short treatment course and obvious curative effect, can be taken conveniently, and is free of toxic and side effects, raw materials of the medicine are cheap and are good in compatibility, sources of the raw materials are abundant, and the preparation process is easy to control, popularize and apply.

The invention discloses a nano-composite for early diagnosis and treatment of gastric cancer and a preparation method of the nano-composite. The nano-composite has a core-shell structure formed by anamphiphilic copolymer, wherein an inner core layer is formed by a distearoyl phosphatidyl ethanolamine fragment, an outer shell layer is formed by a polyethylene glycol fragment, the inner core layerwraps indocyanine green and a hydrophobic gastric cancer chemotherapeutic drug, and the surface of the outer shell layer is connected with RGD short peptides. The nano-composite preparation integratestargeted gastric cancer treatment and fluorescence imaging tracing, targeted aggregation imaging on gastric cancer focus can be implemented, drug release is controlled, the drug concentration of a targeted site is increased, the drug action time is prolonged, and the nano-composite has a wide application prospect in targeted diagnosis and treatment and drug delivery of early gastric cancer.

The invention discloses a chemiluminiscence detection kit for diagnosing gastropathy in a combined manner. The detection kit is mainly used for performing pepsinogen I detection and pepsinogen II detection. On the basis of the detection kit, the invention further provides a marker combined standard value, which is applicable to the detection kit and can provide reference for gastropathy detection, for detection. The detection kit has the advantages that the detection kit is wide in linearity range, high in specificity, convenient to operate, and the like in terms of gastropathy diagnosis and early gastric cancer screening; in addition, the detection kit is accurate and detailed in gastropathy diagnosis and high in market popularization value, and damage, caused by X-rays, on human bodies and the inconvenience of a gastroscope are avoided.

The invention relates to the field of medical diagnostics, in particular to application of a cysteine protease inhibitor SN and Cathepsin L1 compound as a gastric cancer diagnosis marker. Through detection of the CST1-CTSL compound, the tissue specificity of the marker can be effectively improved, and the detection rate of early gastric cancer can further be effectively improved.

The invention discloses traditional Chinese medicine for treating various diseases of the stomach. The traditional Chinese medicine is prepared from the following raw material medicine in parts by weight: 1 part of scorched hawthorn fruit, 1 part of rhizoma bletillae, 1 part of bighead atractylodes rhizome and 1 part of fructus aurantii immaturi. The scorched hawthorn fruit has the effects of improving digestion, strengthening stomach, activating blood circulation, eliminating stasis and relieving dysentery by astringency; the rhizoma bletillae has the effects of stopping bleeding by astringency, diminishing swelling and promoting tissue regeneration; the fructus aurantii immaturi has the effects of relieving stagnant Qi, removing retention, reducing phlegm and removing stasis; the bigheadatractylodes rhizome has the effects of invigorating spleen, supplementing qi and eliminating dampness to alleviate water retention; the four kinds of medicine are matched for jointly achieving the novel functions of removing necrotic tissues and promoting new tissue regeneration. A preparation method of the traditional Chinese medicine comprises the following steps of grinding the raw material medicine according to the weight parts into powder; performing uniform stirring; preparing the raw medicine into traditional Chinese medicine powder or granules; performing packaging according to a dose of 16g per bag. The four kinds of medicine are specially matched; the novel functions of removing necrotic tissues and promoting new tissue regeneration are achieved; various diseases in the stomachcan be effectively treated; the comprehensive effect is particularly good on chronic superficial gastritis, erosive gastritis, gastric ulcer, gastric polyps, early gastric cancer and intestinal metaplasia; the hypokinetic intestines and stomach function can be recovered; the treatment and health care functions are realized.