Culture method of 3D tumor organs for test of gastric cancer

Abstract

The invention discloses a culture method of 3D tumor organs for anti-tumor drug sensitivity test of gastric cancer to solve the problem of low success rate of modeling of existing tumor organs. The method comprises the following specific steps: Step 1, preparation before an experiment; Step 2, tissue sampling; Step 3, tissue enzymatic separation; Step 4, PDO tumor organ modeling; and Step 5, PDO culture. The design of the method is reasonable. By adding gastrin during the culture process, the modeling success rate and cell viability of gastric cancer organs are promoted. It is found through comparison that when the gastrin-added medium is used to culture gastric cancer PDO, the formed PDO mass increases significantly, the cell viability is also increased, and the modeled tumor formation rate is increased significantly to 80%. It shows that the use effect is good and the method can provide a basis for the test of tumor drug sensitivity, screening of anti-tumor drugs for patients and clinical medication.

Description

technical field [0001] The invention relates to the field of gastric cancer testing, in particular to a method for culturing tumor 3D organoids for gastric cancer testing. Background technique [0002] Gastric cancer is one of the most common digestive tract malignancies that seriously threaten human health in China and even in the world, with high morbidity and mortality. According to relevant statistics, in Asia, the death rate of gastric cancer ranks second among malignant tumors. The vast majority of gastric cancer is adenocarcinoma. There are no obvious symptoms in the early stage, or non-specific symptoms such as epigastric discomfort and belching, which are often similar to the symptoms of chronic gastric diseases such as gastritis and gastric ulcer, and are easily ignored. Therefore, the early diagnosis rate of gastric cancer is still low. The vast majority of gastric cancers are diagnosed in the middle and advanced stages. At present, the treatment of tumors is st...

AI Technical Summary

Problems solved by technology

[0004] Establishing a system for judging the sensitivity of tumors to tumor drugs requires the collection of a lot of data. The most authentic data is the tumor organoids of patients. The method of culturing tumor organoids determines the characteristics of tumor organs. The base components include: Advanced DMEM (improved DMEM medium), B27, N2, GlutaMax, Penicilin / streptomycin (penicillin / streptomycin), EGF (growth factor), Noggin, R-Spodin, FGF-10, FGF- basic, Wnt-3A, Prostaglandin E2 (prostaglandin E2), Y-27632, Nicotinamide (nicotinamide), A83-01, SB202190, HGF (hepatocyte growth factor), although this method can obtain tumor organoids, but The biological mechanism of the tumor organ is quite different from the patient's own organ, and the pathological morphology of the patient's tissue cannot be preserved, and the behavior of the original tumor cannot be simulated in the early in vitro experiments, nor can it truly reflect the genetic development, invasion, and metastasis of the tumor. In terms of the biological characteristics of tumors, the efficiency of modeling is relatively low, with only about 20% success rate, which affects the accuracy of people's judgment system

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