Method for inducing non-alcoholic fatty liver disease through fluorine intervention

A non-alcoholic fatty liver technology, applied in the field of non-alcoholic fatty liver model induced by fluoride intervention in mice, can solve the problems of streptozotocin toxicity, long modeling time, and high model cost, and shorten the modeling time. The effect of mold cycle, simple experiment and low cost

Pending Publication Date: 2021-09-28
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] Animal models of NAFLD are mainly divided into (1) high-fat diet-induced non-alcoholic fatty liver model, (2) low-dose streptozotocin combined with high-fat diet-induced non-alcoholic fatty liver model, (3) gene editing Non-alcoholic fatty liver model, the biggest disadvantage of model (1) is that it takes a long time to build the model. The mechanism of non-alcoholic fatty liver in model (2) is quite different from that of human non-alcoholic fatty liver. Moreover, streptozotocin is highly toxic and easily causes animal death, and the cost of the model (3) is relatively high

Method used

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  • Method for inducing non-alcoholic fatty liver disease through fluorine intervention
  • Method for inducing non-alcoholic fatty liver disease through fluorine intervention
  • Method for inducing non-alcoholic fatty liver disease through fluorine intervention

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) Twenty-four SPF grade male C57BL / 6 mice (4 weeks old) were fed freely with food and water for one week and then randomly divided into three groups, with 8 mice in each group. The first group was fed with normal diet (Normal Diet, ND group); the second group was fed with high-fat diet (High-fat Diet, HFD group); the third group was fed with high-fat diet, and the concentration of sodium fluoride added in drinking water was 110mg / L (HFD-F group);

[0020] (2) After 10 weeks of intervention, the mice were fasted for 12 hours, and the mice were sacrificed by pulling the neck through the heart. After centrifugation, the plasma was obtained for biochemical analysis. After the mouse liver was weighed and recorded, it was put into the fixative used to prepare slices;

[0021] (3) Physiological and biochemical indicators in mouse plasma such as TG, TC, LDL-C and HDL-C were determined according to the kit instructions;

[0022] (4) After weighing the liver tissue of the mou...

Embodiment 2

[0025] (1) To verify the mechanism of fluorine-induced non-alcoholic fatty liver, purchase 16 SPF grade male Tlr4 knockout C57BL / 10J mice (4 weeks old) and 16 C57BL / 10J wild-type control mice (4 weeks old ), were randomly divided into 4 groups, with 8 mice in each group, after a week of adaptation to feeding with free access to food and water. The first group of wild type fed high-fat diet group (HFD group); the second group fed with high-fat diet and added sodium fluoride concentration of 110mg / L in drinking water (HFD-F group); the third group was Tlr4 knockout Mice fed high-fat diet group (TLR4-HFD); the fourth group was Tlr4 knockout mice fed high-fat diet group, and added sodium fluoride concentration of 110mg / L in drinking water (TLR4-HFD-F group) ;

[0026] (2) After 8 weeks of intervention, after the mice were fasted for 12 hours, the mice were sacrificed by pulling the neck through the heart, and the plasma was obtained after centrifugation for biochemical analysis. ...

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Abstract

The invention relates to a method for inducing a non-alcoholic fatty liver disease model through fluorine intervention. The method comprises the steps that high-fat diet is combined with fluorine intervention to induce the non-alcoholic fatty liver disease of an animal, the fat content in the high-fat feed is 45-60%, 0.01%-0.2% of sodium fluoride is added into the high-fat feed, or 50-1000 mg/L of sodium fluoride is added into drinking water of the animal, and the intervention period is 2-3 months. The non-alcoholic fatty liver disease animal model is high in modeling rate, stable in performance, low in cost and short in period, and the non-alcoholic fatty liver disease can be systematically and scientifically researched by utilizing the method.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for inducing a non-alcoholic fatty liver model in mice by fluorine intervention. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a chronic disease characterized by excessive accumulation of fat in liver cells caused by non-alcoholic effects. More and more studies have shown that NAFLD can increase the risk of type 2 diabetes mellitus (T2DM), cardiovascular disease (CVD) and other diseases. More and more studies have shown that dietary structure or food composition is an important factor causing non-alcoholic fatty liver. With the development of society and the improvement of people's living standards, people's eating habits have gradually shifted to a "three high" diet of high sugar, high fat, and high calories, which in turn increases the risk of non-alcoholic fatty liver. [0003] Fluorine is an element widely distributed on the earth, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2267/03
Inventor 陈贵杰曾晓雄孙怡
Owner NANJING AGRICULTURAL UNIVERSITY
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