24 results about "Normal diet" patented technology

The present invention provides a food composition comprising a plurality of amino acids or salts thereof wherein the amino acids or salts thereof comprise from about 0.0016 to 0.6 wt% of glycine or salts thereof and from about 0.00109 to 1.2 wt% of serine or salts thereof. Also provided is a method of treating cancer in a subject comprising partially or completely replacing or replacing the normal diet of the subject with the food composition, and optionally administering to the subject an effective therapeutic amount of an anti-PD-1 antibody or an anti-PD-L1 antibody.

The invention discloses a method for establishing a cross-generation genetic model of rats attacking nutritional imbalance twice, and relates to the technical field of animal model establishment, and the key points of the technical scheme are as follows: starting from the angle of paternal line, selecting SD male rats of 3 weeks old, adaptively feeding for 1 week, then starving and feeding for 4 weeks, and finally feeding for 17 weeks with high fat, evaluating the phenotype of fatty liver, and establishing the cross-generation genetic model of rats attacking nutritional imbalance twice. And establishing a parent (F0) which attacks the nutritional imbalance twice. After the parent generation (F0) and the normal exposed parent generation (F0) are mated, the male offspring (F1) is given with common diet and high-fat diet respectively, then after the parent generation (F0) and the normal exposed female generation (F0) are mated, the male offspring (F2) is given with common diet and high-fat diet respectively. According to the method, the fatty liver phenotype of the male rat of the F0-F2 model can be determined, and the glucolipid metabolism pathway and function change of the parental generation and the offspring generation can be known through an isotopic tracing technology, metabonomics and transcriptomics.

The invention belongs to the technical field of construction of experimental animal models, and particularly relates to a construction method and application of a recombinant inbred line type 2 diabetes mellitus rat model, in parent rats, female rats are SD (fa + / +) or Wistart (fa + / +), male rats are Zucker (fa + / -), heterozygote (fa + / -) female rats in filial generations obtained through hybridization are backcrossed with male parents until an F6 generation, and the female rats and the male rats are subjected to cross breeding to obtain the recombinant inbred line type 2 diabetes mellitus rat model. And hybridizing the F6 generation heterozygote (fa < + / ->) female rat with the F6 generation heterozygote (fa < + / ->) male rat to obtain a homozygote (fa <- / ->) SD / Zucker or Wistart / Zucker rat which is the recombinant inbred line type 2 diabetes mellitus rat. The obtained rats cannot express functional leptin receptors, have hyperappetite and autonomous polydipsia and polyphagia, and are very in line with real occurrence scenes of type 2 diabetes mellitus caused by overnutrition of diet under normal diet conditions of human beings.

The invention discloses lignin-triiodothyronine self-assembled particles as well as a preparation method and application thereof. The lignin-triiodothyronine composite nano particles are prepared from lignin and triiodothyronine in a joint self-assembly mode. Experiments prove that single in-situ injection of the lignin-triiodothyronine composite nanoparticles can increase the expression of subcutaneous adipose tissues UCP-1 of normal diet fed mice and reduce adipose cells; single in-situ injection can increase the expression of subcutaneous adipose tissue UCP-1 of a mouse fed with short-term high-fat diet, reduce the weight of adipose tissue, increase the energy consumption of the mouse and improve the glucose tolerance of the mouse; multiple times of in-situ injection can improve obesity and fatty liver caused by long-term high-fat diet. The lignin-triiodothyronine composite nanoparticle provided by the invention has a good application prospect in treating, relieving or improving obesity or metabolic diseases related to the obesity.

This invention discloses a method for constructing a mouse model of non-obese NAFLD in humans under normal dietary conditions. The method involves injecting adeno-associated virus (adenovirus) shIDO2-AAV8 into mice via the tail vein to target and silence IDO2 gene expression in the liver, while simultaneously administering a normal diet. The adenovirus shIDO2-AAV8 is obtained by recombining an IDO2-shRNA sequence inserted into an AAV8 vector. This invention, through tail vein injection of adenovirus shIDO2-AAV8 combined with a normal diet, can construct a mouse model simulating non-obese NAFLD in humans. Compared with traditional dietary induction methods and combined induction methods, the dietary conditions are consistent with daily human intake, more closely reflecting the actual pathological situation in humans, and more closely reproducing the pathogenesis environment and physical state of non-obese NAFLD in humans, making the model more clinically valuable.

The application provides a method for regulating lipid absorption, a composition and application thereof. The present inventors have been committed to the research on the correlation between diet and metabolism, and found in the previous research that compared with before diet restriction, re-feeding after diet restriction can significantly increase the body fat content. Further research shows that the composition of intestinal flora during normal diet after diet restriction changes significantly, and the proportion of lactobacillus increases significantly; high-protein diet after diet restriction can significantly inhibit the increase of the proportion of small intestinal lactobacillus, and the diversity of flora is significantly increased. Clearing the intestinal flora can effectively inhibit the increase of body fat caused by re-feeding after diet restriction. The present inventors have also found that a five-compound combination of a specific lactobacillus strain can significantly increase lipid absorption and fatty acid uptake of white adipose tissue.

The invention discloses a construction method of a chronic atrophic gastritis precancerous lesion animal model, and the construction method comprises the following specific steps: selecting experimental mice, randomly and averagely dividing the experimental mice into a model group and a blank control group, the experimental mice of the model group drink an MNU solution I by themselves and use an MNU solution II for intragastric administration once a week, and the blank control group normally diets; after modeling, dissecting and taking stomach tissues for later use; the MNU solution I is an N-nitroso-N-methylurea solution with the concentration of 290 mg / L to 310 mg / L; the MNU solution II is an N-nitroso-N-methylurea solution with the concentration of 1.6 g / L to 1.7 g / L; according to the method, 14-week modeling can be achieved, intestinal metaplasia, heterodysplasia and even a small number of cancer cells can occur in 14 weeks, and compared with a traditional MNNG combined multi-factor modeling method, the modeling method has the advantages that the modeling method is simplified, experimental errors and individual differences are reduced, the modeling period is shortened, and the modeling success rate is increased.

This invention discloses a method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron, belonging to the field of biotechnology. The method includes: a) after acclimatizing 4-5cm largemouth bass to a normal diet for one week, the normal diet is replaced with a FeSO4·7H2O-supplemented diet (FeSO4·7H2O supplementation amount: 800-3200 mg / kg), while the control group continues to be fed a normal diet; b) after feeding the FeSO4·7H2O-supplemented diet for 14-56 days, the mortality rate from Aeromonas hydrophila infection increases, indicating successful establishment of the largemouth bass model susceptibility to pathogens. This method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron has advantages such as simple operation, high success and survival rates, good stability, and obvious pathological characteristics.

Through experimental studies, the present invention discovered that GPR180 is highly expressed in animal organs, particularly the liver, and that GPR180 gene expression in the liver is upregulated under the pathological conditions of obesity and diabetes in mice. After knocking down GPR180 in the liver, energy metabolism was enhanced and body fat content was reduced in animals on both a normal diet and a high-fat diet. Knocking down GPR180 in the liver also improved fatty degeneration of the liver in animals induced by a high-fat diet, reduced cholesterol levels in plasma and liver, and improved insulin resistance and increased insulin sensitivity in animals on both a normal diet and a high-fat diet. Based on this, GPR180 can be used as a therapeutic target for non-alcoholic fatty liver disease, weight loss or fat reduction, treatment of diabetes, and hypercholesterolemia. GPR180 inhibitors can be used to prepare drugs for treating non-alcoholic fatty liver disease, weight loss or body fat reduction, obesity, diabetes, and hypercholesterolemia.

The invention provides application of an anti-FGL1 monoclonal antibody in preparation of drugs for treating pancreatic cancer liver metastasis, and belongs to the technical field of antibody drugs. The anti-FGL1 monoclonal antibody can be combined with FGL1 protein in a high-specificity mode, the action mechanism of the anti-FGL1 monoclonal antibody in pancreatic cancer liver metastasis treatment is verified through a tumor cell model, a patient-derived tumor organ model and a mouse pancreas in-situ tumor model, and the anti-FGL1 monoclonal antibody can be used for preparing potential drugs for treating pancreatic cancer liver metastasis. Meanwhile, the anti-cancer effects of the FGL1 monoclonal antibody in normal diet mice and high fat diet mice (fatty liver models) are compared, so that the applicable population of the antibody therapy is determined, and a scientific basis is provided for the treatment of the FGL1 monoclonal antibody in pancreatic cancer.

The invention belongs to the technical field of animal model construction, and particularly relates to a hyperuricemia animal model and a construction method thereof.The animal model can remarkably prolong the maintenance time of the model, and modeling through a method for constraining an experimental animal comprises the steps that A, constraint day constraining is conducted, specifically, the experimental animal is constrained for 3-6 hours through a constraining device, and water and food are forbidden in the constraining period; b, in a non-constraint period, normal diet and free water drinking are carried out; and C, the modeling time is at least 21 days. According to an existing modeling method, uric acid can recover to a normal level after drug withdrawal for 2-6 hours, and the maintenance time of the model is very short, however, the hyperuricemia animal model capable of remarkably prolonging the maintenance time of the model is effectively constructed, and after modeling is stopped for 3 days, the uric acid level in mouse plasma is still maintained at a relatively high stage. The construction method can also be matched with existing modeling methods such as high-purine diet and uricase inhibitors, high-pressure living environments and bad living and diet habits are considered, and the construction method conforms to the pathogenesis of hyperuricemia of modern people.

The invention provides a medicinal and edible composition for regulating spleen and stomach and enhancing immunity and application of the medicinal and edible composition, and belongs to the technical field of medicinal and edible compositions. The medicinal and edible composition comprises the following raw materials: Chinese yam, ginseng, gordon euryale seed, pericarpium citri reticulatae, coix seed, millet and malt. The medicinal and edible composition has the effects of supporting healthy qi and invigorating qi and blood, and can regulate the spleen and stomach of people with low immunity to recover the normal functions of the spleen and stomach. By means of the composition with homology of medicine and food for dietetic invigoration, damaged viscera can be gradually recovered, the pathological deviation of viscera deficiency of the human body is corrected, and the composition can be used for people who cannot eat common diet or cannot meet the nutritional requirements by using daily diet, especially people with weak physique after radiotherapy and chemotherapy or post-operation. An important nutrition support effect is achieved in the aspects of rehabilitation, body function maintenance and the like. The medicinal and edible composition disclosed by the invention can also be used for daily life cultivation and health preservation, assists normal diet, has a remarkable effect, and is widely accepted by patients and eaters.

The invention discloses an amino acid and enzyme preparation nutrient mixture for promoting proliferation of gastrointestinal cells of dogs, and belongs to the technical field of pet food. According to the invention, the proliferation of intestinal epithelial cells and the secretion of Gastrin can be effectively promoted by supplementing arginine. In order to enhance the functional effect of the invention, lactase is especially added in the invention. On the basis of normal diet, 0.125%-0.5% of arginine and 0.00375%-0.015% of lactase are complementarily fed every day. The digestion and absorption of the dogs on nutrient substances of staple food grains can be improved, the parasitic quantity of intestinal escherichia coli of the dogs can be reduced, and excrement in a healthy form can be formed. In addition, the nutritional mixture can significantly promote the expression of epidermal growth factors (EGF). The invention discloses a mechanism for promoting proliferation of canine intestinal epithelial cells by arginine and lactase, and provides a new thought for prevention and treatment of canine digestive diseases.

The present application relates to the technical field of animal model construction, and relates to a fetal origin atherosclerosis susceptible model and a construction method and application thereof.The construction method of the fetal origin atherosclerosis susceptible model comprises the following steps: S1, selecting healthy pregnant ApoE- / - mice, subcutaneously injecting dexamethasone twice a day at 16-17 days of pregnancy, the injection amount of dexamethasone is 0.6-0.8 mg / kg of the body weight of the pregnant female mouse, and the pregnant female mouse is free to eat; S2, the pregnant female mouse naturally gives birth to F1 generation, the male and female offspring are weaned and separated into cages at 3-4 weeks after birth, and continue to be normally fed until 6-7 weeks after birth, and then high-fat high-cholesterol feed is given to feed until 18-19 weeks; the fetal origin atherosclerosis susceptible model is obtained.The present application successfully constructs the fetal origin atherosclerosis susceptible model by injecting dexamethasone at a specific time, and the atherosclerosis lesion of the model presents a significant aggravation effect.

The invention belongs to the technical field of animal husbandry medicines, and particularly relates to a wormwood extract, a preparation method and application of the wormwood extract in preparation of a medicine for preventing foot-and-mouth disease of beef cattle. The method specifically comprises the following steps: adding artemisia desertorum into an acetic acid aqueous solution, boiling at 60-100 DEG C for specified time, filtering and cooling; and adding the cooled artemisia desertorum into an ethanol solution, soaking for a second time at room temperature, and carrying out material-liquid separation to obtain a leaching solution, namely the artemisia desertorum extract. The wormwood extract can prevent cattle from being infected with the foot-and-mouth disease, in the high-incidence period of foot-and-mouth disease infection, the morbidity of cattle taking the wormwood extract is only 10%, the morbidity is reduced by 10% compared with that of cattle taking common diet (the morbidity is 20%), the foot-and-mouth disease of cattle is effectively prevented, and no adverse effect is generated on growth and development of the cattle. In addition, the wormwood is wide in source and low in cost, and the preparation method is simple, convenient and suitable for large-scale breeding and popularization.

This invention belongs to the field of experimental animal model construction technology, specifically relating to the construction method and application of a recombinant inbred type 2 diabetic rat model. The parent rats are SD (fa+ / +) or Wistar (fa+ / +) females and Zucker (fa+ / -) males. Heterozygous (fa+ / -) female offspring are backcrossed with the fathers until the F6 generation. Then, F6 heterozygous (fa+ / -) female rats are crossed with F6 heterozygous (fa+ / -) male rats. The resulting homozygous (fa- / -) SD / Zucker or Wistar / Zucker rats are the recombinant inbred type 2 diabetic rats. The rats obtained by this invention do not express functional leptin receptors, exhibit hyperphagia, and spontaneous polydipsia and polyphagia, which closely resembles the real-world scenario of type 2 diabetes caused by overnutrition under normal dietary conditions in humans.

This invention provides a method for constructing a mouse obesity model and its application, relating to the fields of biotechnology and medical animal model technology. This invention is the first to discover and confirm complement. C9 Genetic defects can lead to spontaneous obesity in mice, and based on this, a novel method for constructing an animal model of obesity has been developed. The model constructed using this method exhibits spontaneous obesity even under a normal diet, avoiding the interference of a forced high-fat diet. Under a high-fat diet, the obesity phenotype is accelerated and exacerbated, providing a flexible time window and a stronger phenotype for research. The model constructed using this method can not only be used for obesity research but also extended to the study of its entire spectrum of metabolic complications and the construction of an efficient drug screening platform, possessing significant scientific and commercial value.

The invention discloses dried sleep-aiding three-seven rice noodles as well as a preparation method and application thereof, and belongs to the technical field of sleep-aiding rice noodle foods. The dried sleep-aiding Sanqiao rice noodles comprise the following raw materials in parts by weight: 15-25 parts of pseudo-ginseng powder, 70-80 parts of rice flour, 10-20 parts of buckwheat flour, 5-15 parts of spina date seed powder, 5-15 parts of a grape seed extract, 5-15 parts of orange juice and 5-15 parts of lily powder. Pseudo-ginseng powder is used as a main functional component, and rice meal, buckwheat flour, spina date seed powder, grape seed extract, orange juice and lily powder are used as auxiliary materials to prepare the rice noodles. The health food is simple in process, rich in nutrition, good in taste and free of any preservatives, is an ideal pure natural green food, and is particularly suitable for sub-health groups with insomnia to eat. According to the invention, the normal diet is not influenced, and meanwhile, the insomnia trouble caused by various factors is improved, so that the effects of calming and helping sleep are achieved, and people can keep good sleep.

The invention provides a method for constructing a mouse obesity model and application, relates to the technical field of biotechnology and medical animal models, and discloses a brand-new method for constructing an obese animal model based on discovery and verification that mouse spontaneous obesity can be caused by complement C9 gene defects for the first time. The model constructed by the method can cause spontaneous obesity under normal diet, so that the interference of forced high-fat diet is avoided; obesity phenotype is accelerated and aggravated under high fat diet, and a flexible time window and stronger phenotype are provided for research. The model constructed by the method not only can be used for obesity research, but also can be extended to research of full-spectrum metabolic complications of the obesity and construction of an efficient drug screening platform, and has great scientific research and commercial values.

The invention relates to the technical field of animal model construction, in particular to a fetal-derived atherosclerosis susceptibility model and a construction method and application thereof. The construction method of the fetal-derived atherosclerosis susceptibility model comprises the following steps that S1, a healthy fertilized ApoE- / -mouse is selected, dexamethasone subcutaneous injection is conducted twice a day after 16-17 days of pregnancy, the injection amount of dexamethasone every time is 0.6-0.8 mg / kg of the weight of a fertilized female mouse, and the fertilized female mouse freely diets; s2, the fertilized female rats produce naturally to obtain an F1 generation, weaning is conducted 3-4 weeks after birth, male and female newborn rats are separated into cages, normal diet feeding continues to be conducted till 6-7 weeks after birth, and then the newborn rats are fed with high-fat and high-cholesterol feed for 18-19 weeks; the fetal-derived atherosclerosis susceptibility model is obtained. The dexamethasone is injected for a specific time, a fetal-derived atherosclerosis susceptible model is successfully constructed, and atherosclerosis lesion of the model shows a remarkable aggravating effect.

The invention relates to the technical field of blood glucose monitoring, in particular to a real-time blood glucose monitor and a high-precision blood glucose monitoring method thereof. The method comprises the following steps: acquiring an analysis curve peak of a daytime period every day in a monitoring period, acquiring a normal diet period according to an overlapping degree of time intervals corresponding to the analysis curve peak in different days, and determining the normal diet period according to the overlapping condition of the time intervals corresponding to the analysis curve peak and the normal diet period. Selecting diet peaks in the analysis curve peaks according to morphological characteristics of different analysis curve peaks; and determining an abnormal index according to the peak value difference and morphological characteristic difference between the analysis curve peak and the diet peak, training a neural network according to the analysis curve peak and the abnormal index of each day except the current day in the monitoring period, and predicting the blood glucose condition in the night time period in the current day by using the trained neural network. According to the method, the attention condition of the analysis curve peak in the neural network process is adaptively set based on the abnormal indexes, and the monitoring precision of the blood sugar of the patient is improved.

Provided is a food composition that comprises a plurality of amino acids or the salts thereof, wherein the plurality of amino acids or the salts thereof comprise about 0.0016-0.6 wt % of glycine or the salt thereof and about 0.00109-1.2 wt % of serine or the salt thereof. Also provided is a method for treating cancer in a subject in need thereof, comprising partly or completely substituting or replacing the normal diet of the subject with the food composition, and optionally administering to the subject a therapeutically effective amount of an anti-PD-1 antibody or an anti-PD-Li antibody.

The invention belongs to the technical field of construction of animal models, and particularly relates to a hyperuricemia animal model constructed by utilizing epinephrine and a construction method of the hyperuricemia animal model. The method for constructing the animal model by injecting low-dose epinephrine comprises the following steps: A, injecting low-dose epinephrine into an experimental animal every day; b, normal diet and free water drinking; and C, the molding time is at least 14 days. The hyperuricemia animal model can be successfully constructed by injecting low-dose epinephrine, and the hyperuricemia animal model can also be successfully constructed in cooperation with a modeling method of high-purine diet, a uricase inhibitor and a urate transporter inhibitor. And considering from the aspects of economy, cost and the like, the hyperuricemia animal model can still be successfully constructed by only injecting low-dose epinephrine without additional auxiliary modeling conditions, so that a brand new choice is provided for the animal model construction method.

The present invention provides a preparation method and application of a protein nanocage loaded with tuna peptides, specifically including the construction and modification of ferritin nanocages, and the extraction, screening, and embedding of tuna active peptides. The tuna active peptides are encapsulated in ferritin nanocages modified with ferulic acid and delivered to the intestines in a targeted manner. They interact with anorexigenic hormone receptors, regulate the production of anorexigenic hormones such as cholecystokinin, glucagon-like peptide 1, and PYY, control appetite after a normal diet, and enhance the body's sense of fullness, thereby reducing food intake and weight loss, thereby playing a role in regulating obesity. In the present invention, ferritin nanocages are modified with ferulic acid. The carboxyl and phenolic hydroxyl groups in the ferulic acid interact with the amino end of the outer surface of the ferritin nanocage, giving the ferritin nanocages the function of being resistant to gastric juice environment. They can exist stably in the stomach of the body without being decomposed, thereby being decomposed in the intestines and exerting their precise targeted effects, laying the foundation for anti-obesity research.