9 results about "Normal diet" patented technology

The invention discloses a method for establishing a cross-generation genetic model of rats attacking nutritional imbalance twice, and relates to the technical field of animal model establishment, and the key points of the technical scheme are as follows: starting from the angle of paternal line, selecting SD male rats of 3 weeks old, adaptively feeding for 1 week, then starving and feeding for 4 weeks, and finally feeding for 17 weeks with high fat, evaluating the phenotype of fatty liver, and establishing the cross-generation genetic model of rats attacking nutritional imbalance twice. And establishing a parent (F0) which attacks the nutritional imbalance twice. After the parent generation (F0) and the normal exposed parent generation (F0) are mated, the male offspring (F1) is given with common diet and high-fat diet respectively, then after the parent generation (F0) and the normal exposed female generation (F0) are mated, the male offspring (F2) is given with common diet and high-fat diet respectively. According to the method, the fatty liver phenotype of the male rat of the F0-F2 model can be determined, and the glucolipid metabolism pathway and function change of the parental generation and the offspring generation can be known through an isotopic tracing technology, metabonomics and transcriptomics.

This invention discloses a method for constructing a mouse model of non-obese NAFLD in humans under normal dietary conditions. The method involves injecting adeno-associated virus (adenovirus) shIDO2-AAV8 into mice via the tail vein to target and silence IDO2 gene expression in the liver, while simultaneously administering a normal diet. The adenovirus shIDO2-AAV8 is obtained by recombining an IDO2-shRNA sequence inserted into an AAV8 vector. This invention, through tail vein injection of adenovirus shIDO2-AAV8 combined with a normal diet, can construct a mouse model simulating non-obese NAFLD in humans. Compared with traditional dietary induction methods and combined induction methods, the dietary conditions are consistent with daily human intake, more closely reflecting the actual pathological situation in humans, and more closely reproducing the pathogenesis environment and physical state of non-obese NAFLD in humans, making the model more clinically valuable.

This invention discloses a method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron, belonging to the field of biotechnology. The method includes: a) after acclimatizing 4-5cm largemouth bass to a normal diet for one week, the normal diet is replaced with a FeSO4·7H2O-supplemented diet (FeSO4·7H2O supplementation amount: 800-3200 mg / kg), while the control group continues to be fed a normal diet; b) after feeding the FeSO4·7H2O-supplemented diet for 14-56 days, the mortality rate from Aeromonas hydrophila infection increases, indicating successful establishment of the largemouth bass model susceptibility to pathogens. This method for establishing a largemouth bass model susceptibility to iron overload pathogens using inorganic iron has advantages such as simple operation, high success and survival rates, good stability, and obvious pathological characteristics.

The present application relates to the technical field of animal model construction, and relates to a fetal origin atherosclerosis susceptible model and a construction method and application thereof.The construction method of the fetal origin atherosclerosis susceptible model comprises the following steps: S1, selecting healthy pregnant ApoE- / - mice, subcutaneously injecting dexamethasone twice a day at 16-17 days of pregnancy, the injection amount of dexamethasone is 0.6-0.8 mg / kg of the body weight of the pregnant female mouse, and the pregnant female mouse is free to eat; S2, the pregnant female mouse naturally gives birth to F1 generation, the male and female offspring are weaned and separated into cages at 3-4 weeks after birth, and continue to be normally fed until 6-7 weeks after birth, and then high-fat high-cholesterol feed is given to feed until 18-19 weeks; the fetal origin atherosclerosis susceptible model is obtained.The present application successfully constructs the fetal origin atherosclerosis susceptible model by injecting dexamethasone at a specific time, and the atherosclerosis lesion of the model presents a significant aggravation effect.

The invention belongs to the technical field of animal husbandry medicines, and particularly relates to a wormwood extract, a preparation method and application of the wormwood extract in preparation of a medicine for preventing foot-and-mouth disease of beef cattle. The method specifically comprises the following steps: adding artemisia desertorum into an acetic acid aqueous solution, boiling at 60-100 DEG C for specified time, filtering and cooling; and adding the cooled artemisia desertorum into an ethanol solution, soaking for a second time at room temperature, and carrying out material-liquid separation to obtain a leaching solution, namely the artemisia desertorum extract. The wormwood extract can prevent cattle from being infected with the foot-and-mouth disease, in the high-incidence period of foot-and-mouth disease infection, the morbidity of cattle taking the wormwood extract is only 10%, the morbidity is reduced by 10% compared with that of cattle taking common diet (the morbidity is 20%), the foot-and-mouth disease of cattle is effectively prevented, and no adverse effect is generated on growth and development of the cattle. In addition, the wormwood is wide in source and low in cost, and the preparation method is simple, convenient and suitable for large-scale breeding and popularization.

This invention provides a method for constructing a mouse obesity model and its application, relating to the fields of biotechnology and medical animal model technology. This invention is the first to discover and confirm complement. C9 Genetic defects can lead to spontaneous obesity in mice, and based on this, a novel method for constructing an animal model of obesity has been developed. The model constructed using this method exhibits spontaneous obesity even under a normal diet, avoiding the interference of a forced high-fat diet. Under a high-fat diet, the obesity phenotype is accelerated and exacerbated, providing a flexible time window and a stronger phenotype for research. The model constructed using this method can not only be used for obesity research but also extended to the study of its entire spectrum of metabolic complications and the construction of an efficient drug screening platform, possessing significant scientific and commercial value.

The invention discloses dried sleep-aiding three-seven rice noodles as well as a preparation method and application thereof, and belongs to the technical field of sleep-aiding rice noodle foods. The dried sleep-aiding Sanqiao rice noodles comprise the following raw materials in parts by weight: 15-25 parts of pseudo-ginseng powder, 70-80 parts of rice flour, 10-20 parts of buckwheat flour, 5-15 parts of spina date seed powder, 5-15 parts of a grape seed extract, 5-15 parts of orange juice and 5-15 parts of lily powder. Pseudo-ginseng powder is used as a main functional component, and rice meal, buckwheat flour, spina date seed powder, grape seed extract, orange juice and lily powder are used as auxiliary materials to prepare the rice noodles. The health food is simple in process, rich in nutrition, good in taste and free of any preservatives, is an ideal pure natural green food, and is particularly suitable for sub-health groups with insomnia to eat. According to the invention, the normal diet is not influenced, and meanwhile, the insomnia trouble caused by various factors is improved, so that the effects of calming and helping sleep are achieved, and people can keep good sleep.

The invention provides a method for constructing a mouse obesity model and application, relates to the technical field of biotechnology and medical animal models, and discloses a brand-new method for constructing an obese animal model based on discovery and verification that mouse spontaneous obesity can be caused by complement C9 gene defects for the first time. The model constructed by the method can cause spontaneous obesity under normal diet, so that the interference of forced high-fat diet is avoided; obesity phenotype is accelerated and aggravated under high fat diet, and a flexible time window and stronger phenotype are provided for research. The model constructed by the method not only can be used for obesity research, but also can be extended to research of full-spectrum metabolic complications of the obesity and construction of an efficient drug screening platform, and has great scientific research and commercial values.

The invention relates to the technical field of animal model construction, in particular to a fetal-derived atherosclerosis susceptibility model and a construction method and application thereof. The construction method of the fetal-derived atherosclerosis susceptibility model comprises the following steps that S1, a healthy fertilized ApoE- / -mouse is selected, dexamethasone subcutaneous injection is conducted twice a day after 16-17 days of pregnancy, the injection amount of dexamethasone every time is 0.6-0.8 mg / kg of the weight of a fertilized female mouse, and the fertilized female mouse freely diets; s2, the fertilized female rats produce naturally to obtain an F1 generation, weaning is conducted 3-4 weeks after birth, male and female newborn rats are separated into cages, normal diet feeding continues to be conducted till 6-7 weeks after birth, and then the newborn rats are fed with high-fat and high-cholesterol feed for 18-19 weeks; the fetal-derived atherosclerosis susceptibility model is obtained. The dexamethasone is injected for a specific time, a fetal-derived atherosclerosis susceptible model is successfully constructed, and atherosclerosis lesion of the model shows a remarkable aggravating effect.