The invention discloses a separately powered dynamic blood sugar monitoring transmitter, a system and a signal sampling method. The separately powered dynamic blood sugar monitoring transmitter structurally comprises a transmitter body and a sensor base device. The sensor base device is divided into a base and adhesive tape, a battery jar and a groove are formed in the base, a battery is arrangedin the battery jar, a rotating seat is installed in the groove, one side of the rotating seat is hinged in the groove, a silica gel seat is arranged in the rotating seat, a pair of conductive rubbersis arranged in the silica gel seat, the transmitter body comprises a circuit board assembly, and the circuit board assembly is provided with four conductive pins which can be in contact with a positive electrode, a negative electrode and the two conductive rubbers. Compared with the prior art, the button battery can be separated from the transmitter body and integrated with a sensor, when the transmitter is not used, the transmitter is not installed on a sensor base, so that the transmitter is in a power-off state, power of the button battery is not consumed; when the transmitter is used, thetransmitter is buckled on the sensor base, and the battery on the sensor base is electrically connected to the transmitter for power supply.