45 results about "Cellular component" patented technology

A sequence of microscopic images is received that visualizes fluorescently labeled cellular components in living cells, each image containing an array of pixels. The images are segmented to identify one or more cells visualized therein. The machine learning model assigns a cell health condition score and / or a cell health subtype score for each pixel of a particular cell. These pixel-based cellular health scores and / or cellular health subtype scores are used to calculate an overall cellular health score and / or an overall cellular health subtype score for each cell. Data characterizing the calculated score is provided to the consumer application or process.

The present disclosure is generally directed to anchoring bridge nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid, contacting the anchored bridge probes with primary probes that hybridize with the bridge probes and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

The sperm optimal tube comprises an upper tube, a lower tube and an inner tube, the upper tube and the inner tube are communicated, the upper tube is detachably connected with the lower tube, when the upper tube is connected with the lower tube, the lower tube is located outside the inner tube, one end of the inner tube is communicated with the upper tube, and the other end is provided with an opening allowing sperm to pass through, and a switch valve is arranged on the opening, the switch valve can be opened under the action of centrifugal force and closed after the centrifugal force stops. The device can effectively separate normal and active sperm, and especially because the switch valve is arranged, the sperm is allowed to flow out under the action of centrifugal force, and the switch valve is closed to seal the inner tube when the centrifugal force is eliminated, so that the abnormal sperm, the inactivated sperm and other cell components in the semen in the inner tube are prevented from entering the lower tube, and the complete separation of sperm with different activities is realized; the design of the inner tube, the upper tube and the lower tube facilitates the disassembly of the outer tube, the semen is more convenient to take out, and the mutual interference is avoided.

The present invention relates to a cellular component homogenate in liquid form, as well as to a cellular component homogenate in solid form, preferably as sprayed powder. Furthermore, the present invention relates to a homogenisation process for the preparation of said cellular component homogenate in liquid form and said cellular component homogenate in solid form, preferably as sprayed powder. Lastly, the present invention relates to a composition comprising said cellular component homogenate in solid form and, optionally, one or more pharmaceutical or food grade or cosmetic additives and excipients, for use in the pharmaceutical, nutraceutical, medical devices, foods for special medical purposes, dietary supplements and food industry both in the human and veterinarian field, as well as for use in the cosmetics industry.

The application discloses a preparation method of a decellularized matrix and a product prepared by the method. The preparation method comprises the following steps: (1) washing a mammalian soft tissue to remove blood, lipids and impurities, and cutting the tissue into pieces with a thickness of 1-10 mm; (2) soaking the pieces in solution 1 for 6-48 h, and then soaking the pieces in solution 2 for 0.1-1 h; (3) treating the pieces in an ultrasonic water bath under the condition of a frequency of 20-40 kHz for 5-30 min; (4) water-bathing the pieces at 55-65 DEG C for 10-30 min; (5) sequentially washing the tissue with deionized water, a PBS buffer and ethanol, and washing the tissue with each solution for 1-3 times, each time for 10-30 min; and (6) freeze-drying the decellularized matrix material, and crushing the material at-80 DEG C to-20 DEG C to obtain a decellularized matrix powder. The preparation method significantly improves the cell component removal efficiency, effectively protects structural proteins such as collagen from being degraded in the freeze-drying and crushing processes, and ensures the integrity of the matrix in the aspects of morphology, structure and function, thereby providing a structural support and biological signal support for subsequent tissue repair.

Aspects of the present disclosure relate to the use of engineered host cells capable of producing protocatechuic acid (PCA). Aspects of the present disclosure also relate to compositions comprising engineered host cells capable of producing protocatechuic acid and / or cell constituents thereof, and the use of such compositions for altering and / or maintaining biological activity in a subject.

The present invention relates to: a fish-derived acellular dermal matrix having improved tissue-to-tissue adhesion problems while ensuring biocompatibility by removing cellular components from fish-derived by-products; and a preparation method therefor.

Assays for determining the risk that a compound will induce liver injury in a subject are provided. A data set comprising a plurality of cellular component abundance values for a plurality of cellular components for each of a plurality of cells exposed to a test chemical compound for a first period of time is obtained. The data set is used to determine a pass value for each of a plurality of pass modules. Each module contains an independent subset of the plurality of cellular components. The pass values are input into one or more first models, thereby obtaining one or more first values. The abundance value is input into one or more second models, thereby obtaining one or more second values. The one or more first values and the one or more second values determine the risk that the compound will induce the liver injury.

A computational system and method for digital modeling of biochemical reactions within cardiac cells are disclosed. The system comprises processors and a computer-readable storage device with instructions to maintain biological data related to cardiac cell reactions and receive user selections via a user interface to configure a simulation. The user can select a cardiac cell class, and functions to load cellular components, generate enzyme activity, load specific enzymes, and perform enzymatic reactions. The system performs in silico experiments, predicts new biological data, and compiles this into configuration data. An enzyme activity model is generated and simulated under various conditions, including user-defined enzymatic dysregulation, to replicate and study cardiac physiology and pathology. The results, such as changes in energy metabolism parameters like glucose consumption and ATP usage, are outputted. The system provides a detailed and interactive platform for research and education in cardiac electrophysiology and disease.

The present invention relates generally to a method of detecting inhibitory effects of health supplements on serine protease in cell lines derived from human carcinomas. More, particularly, the present invention relates to inhibitory analysis of health supplementary products taken from market and / or chemically purified forms, in order to determine their competitors and / or inhibitory effects on serine protease such as trypsin so that these supplements can be used for the treatment once someone develop intense inflammatory reactions either due to infections like COVID-19 and / or pathological conditions such as cancer. The present invention further provides therapeutic applications for agents and / or condition which utilize human enzymatic system and / or cellular components for the development of pathological conditions.

To provide methods that provide a highly sensitive method for detecting rare cell types in a cell population and can overcome limited sensitivity in conventional methods of detecting stem cells caused by background expression of stem cell genes in non-stem cells and by cell loss during sample preparation.SOLUTION: A method of detecting the presence of or confirming the absence of target cells in a cell population comprises: specific steps (a) to (e).SELECTED DRAWING: None

The present invention relates to the strain Lactococcus lactis subsp. lactis CECT 31096, to its cellular components, metabolites, and secreted molecules, and to compositions comprising the above products, as well as to their uses as a medicament, preferably for the prevention and / or reduction of hypertension, or as a food composition or nutraceutical.

The application discloses a dislocation tooth storage and treatment box and a storage and treatment method. The box comprises a box body and a box cover. One side of the box cover is hinged to the box body, and the other side of the box cover is detachably connected to the box body. The box body is internally provided with a cleaning groove, a liquid storage groove, a tooth storage groove and a storage groove. The cleaning groove and the tooth storage groove penetrate through the bottom of the box body, and a sealing plug is detachably connected to the bottom of the box body. The cleaning groove, the liquid storage groove and the tooth storage groove are respectively detachably connected with a cleaning groove cover, a liquid storage groove cover and a tooth storage groove cover, which are fixedly connected to the box cover. A pair of tweezers is detachably connected to the storage groove. The scheme uses physiological saline to flush dirt on the dislocation tooth, uses HBSS to maintain and recombine cell components consumed by periodontal membrane cells, provides a good storage environment, uses HBSS containing ALN to treat the dislocation tooth to reduce root absorption after replantation, avoid bone adhesion and improve the replanting success rate.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

The invention belongs to the technical field of biological medicine, and discloses application of THY1 in diagnosis and prognosis of glioblastoma. According to the invention, a THY1-positive cancer-related fibroblast (THY1 + CAF) subgroup is identified in glioblastoma for the first time, and it is found that the subgroup and tumor blood vessels are highly co-localized in spatial distribution, and the abundance of the subgroup and the blood vessel density are significantly positively correlated; tHY1 + CAF is a key cell component for promoting angiogenesis in a GBM tumor microenvironment; tHY1 can be used as a marker of GBM specific CAFs, and is used for auxiliary diagnosis, prognosis evaluation (high THY1 + CAF may indicate worse prognosis and stronger angiogenesis phenotype) and curative effect monitoring of GBM. Meanwhile, the target THY1 or THY1 + CAF provides a brand-new and potential action target for developing a GBM new therapy for resisting angiogenesis and even remodeling an immunosuppressive microenvironment.

Disclosed herein include systems, methods, compositions, and kits for generating protected cellular component-binding compositions. There are provided, in some embodiments, protected cellular component-binding compositions comprising a cellular component-binding reagent associated with a protected cellular component-binding reagent specific oligonucleotide. A protected cellular component-binding reagent specific oligonucleotide can comprise a terminal blocked region. In some embodiments, the 3′ end of the protected cellular component-binding reagent specific oligonucleotide is incapable of being extended by a reverse transcriptase or a polymerase.

A system and method for distinguishing non-failing from failing cardiac fibroblasts (CFs) using advanced imaging techniques and machine learning (ML). The method involves staining CFs with fluorescent dyes to highlight key cellular components, such as nuclei and actin fibers. Images are analyzed to extract morphological features, which are then processed and normalized. A supervised ML system, trained on labeled CF data, classifies CFs based on phenotypic differences. The system enables accurate diagnosis of heart failure (HF), facilitates screening for therapeutic efficacy, and identifies antifibrotic agents by evaluating changes in CF phenotypes pre- and post-treatment.

A targeting delivery system loaded with whole-cell components, which relates to the technical field of immunotherapy. The targeting delivery system is a nano-sized or micron-sized particle with a target head on the surface, and the particle is loaded with whole-cell components of cancer cells or cancer tissues; the whole-cell components are water-soluble ingredients and water-insoluble ingredients of whole cells in cells or tissues, and the water-insoluble ingredients are dissolved by a solubilizer; and the target head binds to a molecule on the surface of a specific cell or tissue, so as to help the particle to enter the cell or tissue. According to the targeting delivery system, a specific solubilizer is used to solubilize the water-insoluble part, which allows same to be dissolved in an aqueous solution, so that the whole-cell antigens of the water-soluble ingredients and the water-insoluble ingredients in cancer cells or tissues can be combined to prepare a cancer vaccine. In addition, the target head capable of targeting antigen-presenting cells is added to improve the phagocytosis efficiency of the antigen-presenting cells in an active targeting manner, thereby improving the effect of preventing or treating cancer.

A sequence of microscopic images, each comprising an array of pixels, visualizing fluorescently labeled cellular components within live cells is received. These images are segmented to identify one or more cells visualized within the images. A machine learning model assigns a cell health score and / or cell health subtype score to each pixel of a particular cell. The cell health scores and / or cell health subtype scores based on these pixels are used to calculate a total cell health score and / or total cell health subtype score for each cell. Data characterizing the calculated scores is provided to a consuming application or process.

The invention discloses an airway stent and a preparation method thereof. The airway stent comprises a stent skeleton and a decellularized biological membrane layer which covers the outer surface of the stent skeleton and is derived from animal tissues, the stent skeleton is of a tubular structure which is formed by winding a continuous nickel-titanium memory alloy wire and provided with a continuous wave-shaped ring, different supporting forces are provided at different positions by arranging a dense section and a sparse section, cell components are completely removed from the decellularized biological membrane layer, and immunological rejection is avoided.

The invention belongs to the technical field of biomedical materials, and particularly relates to a clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane and application thereof. The clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane provided by the invention is mainly composed of autologous adipose tissue-derived collagen, and contains a plurality of key adhesion proteins, glycosaminoglycans, growth factors and other bioactive components. The preparation method comprises the following steps: by taking autologous adipose tissue as a raw material, breaking cells by a mechanical method, repeatedly cleaning to remove cell components such as cell debris and oil drops, and mechanically pressing through a mold to form a diaphragm with uniform thickness, regular shape and rough surface. The membrane effectively overcomes the limitation of the existing acellular matrix biological membrane, can load various bioactive substances to enhance the function, can be stored at low temperature for a long time without influencing the repair effect, and greatly enhances the convenience of subsequent use.

A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.

Centrifugation based analysis cartridges for analyzing non-cellular constituents of a biological sample are provided. An analysis cartridge is centrifuged around a vertical axis in multiple steps including: a first centrifugation that moves a diluent from a diluent chamber into a diluent metering chamber, a second centrifugation that moves into a mixing chamber the diluent from the diluent metering chamber and a sample from a sample metering chamber; a third centrifugation that moves into one or more metering fingers the diluted samples from the mixing chamber; and a fourth centrifugation that moves into one or more detection chambers the diluted sample from the one or more metering fingers. The connections between the different chambers allow flow of liquids only under centrifugation forces above predetermined levels. Methods of analyzing biological samples using the analysis cartridges disclosed herein as well as analyzers that analyze biological samples by manipulating the analysis cartridges are provided.

The present application belongs to the technical field of biological valve, and relates to a pericardial tissue decellularization method for biological valve preparation, which comprises the following steps: first, using a phased decompression method to decompress to a target vacuum state; then, performing vacuum-assisted decellularization treatment on the pericardial tissue; then, performing washing treatment on the pericardial tissue after the decellularization treatment; finally, performing pressure treatment on the pericardial tissue after the washing treatment, so as to complete the cell removal of the pericardial tissue; and the washing treatment is performed in a periodic pressure difference auxiliary washing mode. The present application can effectively remove cell components, effectively maintain the integrity of the extracellular matrix, and reduce residues in the decellularization process.

Analysis cartridges for analyzing cellular and non-cellular constituents of a biological sample are provided. A cellular analysis module can comprise an imaging chamber comprising a top panel and a bottom panel separated by a suitable distance to produce a monolayer of cells between the panels. A chemical analysis module can comprise a sample distribution channel that distributes a non-cellular portion of a biological sample into a plurality of detection chambers that analyze a plurality of non-cellular constituents. The cellular analysis module can be stacked above the chemical analysis module and vice versa or the two modules can be in substantially the same horizontal plane. A filtration module filters the biological sample as the biological sample transfers from the cellular analysis module through the filter to the chemical analysis module. Methods of analyzing a biological sample using the cartridges disclosed herein are also provided.

This invention discloses a method for decellularizing milkfish skin to produce a skin patch. The method involves a series of steps, comprising: (1) pretreatment of milkfish skin; (2) deodorization; (3) decellularization; (4) decolorization; (5) extensive washing; (6) lyophilization; and (7) sterilization. The present method employs a simpler processing approach while ensuring the removal of cellular components and preserving the structural and biological integrity of the extracellular matrix. This makes the milkfish skin patch suitable for various applications in tissue engineering and regenerative medicine.

This invention provides an information processing device, an information processing method, and an information processing program that can objectively evaluate structural changes in decellularized tissue. [Solution] The information processing device 10 includes a processor, which acquires a first image, which is an image of biological tissue, generates a pseudo-image by applying predetermined image processing to the first image, constructs a structure map showing the distribution of feature quantities of the first image and the pseudo-image, acquires a second image, which is an image of decellularized tissue from which cellular components have been removed from the biological tissue, maps the feature quantities of the second image to the structure map, and calculates a distance scale between the feature quantities of the second image in the structure map and predetermined reference points in the structure map.

The invention discloses a preparation method of an acellular matrix and a product prepared by the preparation method. The preparation method comprises the following steps: (1) cleaning mammalian soft tissues, removing blood, lipid and impurities, and cutting the mammalian soft tissues into thick slices of 1-10mm; (2) soaking in a solution 1 for 6-48 hours, and then soaking in a solution 2 for 0.1-1 hour; (3) putting into an ultrasonic water bath, and treating for 5-30 minutes under the frequency condition of 20-40 kHz; (4) performing water bath for 10-30 minutes under the condition of 55-65 DEG C; (5) respectively and sequentially washing the tissues with deionized water, a PBS buffer solution and ethanol, washing for 1-3 times with each solution, and washing for 10-30 minutes each time; and (6) freeze-drying the acellular matrix material, and crushing at-80 DEG C to-20 DEG C to obtain acellular matrix powder. According to the preparation method disclosed by the invention, the removal efficiency of cell components is remarkably improved, structural proteins such as collagen are effectively protected from being degraded in the freeze-drying and crushing processes, the integrity of the matrix in the aspects of form, structure and function is ensured, and a structural scaffold and biological signal support are provided for subsequent tissue repair.

The invention relates to the B. longum strain CECT 30763, to its cellular components, metabolites, and secreted molecules, and to compositions comprising the above-mentioned products, as well as to uses thereof in the prevention or treatment of mood disorders, such as depression and anxiety, cardiometabolic disorders or liver disorders, alone or presenting as comorbidities.