149 results about "Cellular array" patented technology

A composite filtration medium web of fibers containing a controlled dispersion of a mixture of sub-micron and greater than sub-micron diameter polymeric fibers is described. The filtration medium is made by a two dimensional array of cells, each of which produces a single high velocity two-phase solids-gas jet of discontinuous fibers entrained in air. The cells are arranged so that the individual jets are induced to collide in flight with neighboring jets in their region of fiber formation, to cause the individual nascent fibers of adjacent jets to deform and become entangled with and partially wrap around each other at high velocity and in a localized fine scale manner before they have had an opportunity to cool to a relatively rigid state. The cells are individually adjusted to control the mean diameters, lengths and trajectories of the fibers they produce. Certain cells are adjusted to generate a significant percentage of fibers having diameters less than one micron diameter, and which are relatively shorter in length and certain other cells are adjusted to generate a significant percentage of structure-forming reinforcing fibers having diameters greater than one micron diameter which are relatively longer in length. By employing appropriate close positioning and orientation of the cells in the array, the sub-micron fibers are caused to promptly entangle with and partially wrap around the larger reinforcing fibers. The larger fibers thereby trap and immobilize the sub-micron diameter fibers in the region of formation, to minimize the tendency of sub-micron diameter fibers to clump, agglomerate, or rope together in flight. Also, the larger fibers in flight are made to form a protective curtain to prevent the sub-micron fibers from being carried off by stray air currents.

An automatic vending machine and method are presented. The machine comprises a cells' arrangement having an arrays of cells, each for storing an article therein; a monitoring system comprising an article identifier assembly configured and operable for identifying an article-related data in each of the cells; an article delivering assembly comprising an article holder for receiving a predefined article removed from the respective cell and allowing delivery of the removed article to a customer; and a control system connectable to the article identifier assembly and to the article delivery assembly for selectively operating these assemblies and recording data indicative of articles currently stored in the cells' arrangement and of article removed from the cells' arrangement.

This invention provides isolated nucleic acid molecules encoding a mammalian glycine transporter, isolated nucleic acid molecules encoding a human glycine transporter, isolated proteins which are mammalian glycine transporter proteins, isolated proteins which are human glycine transporter proteins, vectors comprising isolated nucleic acid molecules encoding a mammalian or a human glycine transporter, mammalian cells comprising such vectors, antibodies directed to a mammamlian glycine transporter, antibodies directed to a human glycine transporter, nucleic acid probes useful for detecting nucleic acid encoding mammalian glycine transporter, nucleic acid probes useful for detecting nucleic acid encoding human glycine transporter, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a mammalian glycine transporter, antisense oligonucleotides complementary to any sequences of a nucleic said molecule which encodes a human glycine transporter, pharmaceutical compounds related to mammalian glycine transporter and nonhuman transporter, pharmaceutical compounds related to human glycine transporter and nonhuman transgenic animals which express DNA encoding a normal or a mutant mammalian or human glycine transorter. This invention also provides methods for determining ligand binding, detecting expression, drug screening, and treatments for alleviating abnormalities associated with mammalian glycine transporter. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatments for alleviating abnormalities associated with human glycine transporter.

A method of forming an array of viable cells is carried out by ink-jet printing a cellular composition containing said cells on a substrate. At least two different types of viable mammalian cells are printed on the substrate, the at least two different types of viable mammalian cells selected to together form a tissue. In some embodiments at least three or four different viable mammalian cells are printed on the substrate, the cells selected to together form a tissue. In some embodiments one of the viable mammalian cell types is a stem cell. In some embodiments the method further comprises printing at least one support compound on the substrate, the support compound selected to form a tissue together with said cells. In some embodiments the method further comprises printing at least one growth factor on the substrate, the growth factor selected to cause the cells to form a tissue.

A built-in replacement analysis (BIRA) circuit allocates spare rows and columns of cells for replacing rows and columns of an array of memory cells in response to an input sequence of cell addresses, each identifying a row address and a column address of each defective cell of the cell array. The BIRA subsystem, including a row register corresponding each spare row and a column register corresponding to each spare column, responds to incoming cell addresses by writing their included row address into the row registers, by writing their column addresses into the column registers, and by writing link bits into the column registers. Each link bit links a row and a column register by storing row and column addresses of a defective cell. The BIRA subsystem also writes a "multiple cell" bit into each row register to indicate when the row address it stores includes more than one defective cell. The row and column addresses stored in these registers indicate the array rows and columns for which spare rows and columns are to be allocated. Each row and column register also includes a "permanent" bit the BIRA subsystem sets to indicate when the spare row or column allocation indicated by its stored row or column address is permanent. The BIRA subsystem efficiently allocates spare row and columns by manipulating the data stored in the row and column registers in response to a sequence of defective cell address.

A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

Arrays of single cells and methods of producing an array of single cells are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single cell capture, detection and quantitation.

Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyses on the formed cell arrays.

A visual Reference tag is formed from an array of cells, wherein each cell is visually distinct from all other cells in a given neighborhood and each of said cells contains a single visual cue, for example a unique color, selected from a finite number of visual cues.

There is provided a semiconductor storage device which is capable of further reducing a size of a memory cell, and increasing a storage capacity. Plural memory cells each including a transistor formed on a semiconductor substrate, and a variable resistive device having a resistance value changed by voltage supply and connected between source and drain terminals of the transistor are arranged longitudinally and in an array to configure a three-dimensional memory cell array. A memory cell structure has a double channel structure in which an inside of a switching transistor is filled with a variable resistance element, particularly, a phase change material. The switching transistor is turned off by application of a voltage to increase a channel resistance so that a current flows in the internal phase change material to operate the memory.

Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

The invention discloses a super-hydrophobic micro-pit array chip and a preparation method and application thereof. The super-hydrophobic micro-pit array chip comprises a micro-pit array layer, and the surface, except micro-pits or the bottoms of the micro-pits, of the micro-pit array layer is the super-hydrophobic surface. The super-hydrophobic micro-pit array chip can be characterized in that the surface except the micro-pits is the super-hydrophobic surface, and the super-hydrophobic micro-pit array chip comprises the micro-pit array layer and a super-hydrophobic layer attached to the surface of the micro-pit array layer; the surface except the bottoms of the micro-pits is the super-hydrophobic surface, the micro-pit array layer comprises a base layer and a micropore array layer attached to the surface of the base, and micropore array layer is made of a super-hydrophobic material. The super-hydrophobic micro-pit array chip can achieve complete isolation among cell microarrays at a low cost in the cell microarray construction process, cross contamination of the cell arrays is avoided, good biocompatibility is guaranteed, and the super-hydrophobic micro-pit array chip has no obvious influence on normal growth of various cells and is suitable for high-throughput cell detection and analysis.

This invention is directed to the use of focused energy, particularly focused acoustic energy, in the spatially directed ejection of cells suspended in a carrier fluid, e.g., for providing a pattern of cells on a substrate surface, such as a cellular array.

The invention discloses a Chinese character password encoding method based on image pixel coordinates. The encryption process comprises the steps that (1), plaintext Chinese character strings are input, and the length K of each character string is fetched; (2), a public key image f1 and a private key image f2 are read to generate a private key image F, and the size M*N of the image is fetched; (3), each character is converted into binary data and segmented into a high byte block and a low byte block, and the high byte block and the low byte block are stored into a cellular array destr (i, 1) and a cellular array destr (i, 2) respectively; (4), pixel values matched with the cellular array destr (i, 1) and the cellular array destr (i, 2) are searched for in a circulating mode in the F, and pixel coordinates are read and stored into a two-dimensional matrix left (x, y) and a two-dimensional matrix right (x, y); (5), two coordinate values in the left (x, y) and the right (x, y) are fetched randomly for replacing the high byte block and the lower byte block of the character; (6), positions of four values of the two coordinates are changed according to an appointed position changing sequence, and the four values are output into a four-dimensional matrix (x, y, v); (7), when i is equal to K, the encryption process is finished, and the K-line four-dimensional matrix (x, y, v) is output to be a ciphertext. The encryption method has the advantages that privacy is good, and private key management is flexible and convenient, and is the safe Chinese character password encoding method.

A lighting device including one or more solid state light sources having an electronically adjustable light beam distribution is disclosed. The lighting device may be a lamp configured to include one or more light source modules, each including one or more solid-state emitters populated over a printed circuit board (PCB). The lamp further may include one or more optics configured to modify the output of its one or more light source modules. For a given module, the one or more emitters thereof may be arranged, for example, in a matrix, cellular array, concentric array, or other arrangement, as desired for a given target application or end-use. A given emitter may be addressable individually, in one or more groupings, or both. In some cases, a lamp provided as described herein may be configured for retrofitting existing lighting structures.

If RF transmission repeater units could be mounted in as many automobiles as possible, particularly automobiles owned by drivers residing in low population regions, the likelihood would increase that there could be established wireless transmission paths between a wireless telephone unit and cellular array base stations, including a set of at least one automobile mounted repeater unit intermediate and independent of said wireless telephone unit and said base station. With enough automobiles with mounted repeaters travelling in the remoter regions, there would be a reasonable likelihood that such sequential sets of repeaters connecting to base towers of adjacent cellular arrays could be randomly established. The situation could occur that two or more alternate paths could be establishable between a cellular telephone and cell base stations via two different sets of repeaters. In such a case, as set forth hereinafter in greater detail, there are likely to be different cell base stations, each for a different path. In such a case, any conflict could be resolved by selecting the path having the best transmission attributes. This conflict could readily be resolved through conventional cellular telephone system technology that switches moving cell phones within cellular array areas that “hand-off” or switch a moving cell phone as it moves from conventional cell to cell. This hand-off is based upon attributes like signal-to-noise ratio or strength of signal.

A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

A method for implementing neural network calculations using a cell array computing system, the cell array computing system comprising: a main control CPU, a cell array, and a cell array bus; dimensional array; the main control CPU communicates with each cell in the cell array through the cell array bus; there is a communication interface between adjacent cells, and data can be sent to each other; the method includes: store one or more neuron function codes; any cell participating in the neural network calculation receives the execution result of the neuron function code output from other cells, and executes the code stored in the cell as the input data of the cell The code of the neuron function, and output the execution result, or store it in the preset address. The invention can overcome the communication bottleneck between the CPU, memory and storage in the existing computer architecture, and improve the overall performance of the system.

The present invention is directed to a screening platform employing a miniaturized three-dimensional cell chip for high-throughput toxicology screening of test and lead compounds, prodrugs, drugs and P-450 generated drug metabolites. To this end, the three-dimensional cell chip, employs human cells encapsulated in a matrix (e.g., collagen or alginate gels) in volumes as small as 10 nL arrayed on a functionalized substrates (e.g., glass microscope slides) for spatially addressable screening against multiple test compounds. With the present platform, over 3,000 cell-matrix islands may be spotted providing for simultaneous screening against multiple compounds at multiple doses and in high replicate.

A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines).

This invention is directed to the use of focused energy, particularly focused acoustic energy, in the spatially directed ejection of cells suspended in a carrier fluid, e.g., for providing a pattern of cells on a substrate surface, such as a cellular array.

The electroporation array is comprised of three technologies: microwire glass electrodes, microelectronic multiplexer stimulator chips and microfluidic flow chamber. Various substances, such as genes, gene silencing RNAi, gene inhibition agents or drugs, can be perfused into the microfluidic flow chamber. The entry of the various substances into the cells will be facilitated by electroporation. An applied electric potential causes nanoscale pores to open in the cell membrane allowing substances in the solution to freely diffuse into the cell. The specific cells selected for electroporation are defined using the computer controlled microelectronic stimulator array. An “image” of which electrodes within the array to apply the electric potential to, and thus electroporate, is de-multiplexed onto the array. All the selected electrodes deliver a current pulse varied by the intensity of the de-multiplexed “image”. By serially perfusing different substances across the cells or tissue and electroporating different areas of the cell or tissue culture, it will be possible to have different cells within the culture contain different genes, gene silencing RNAi, gene inhibition agents, drugs, chemicals or other substances or sets thereof. It is also possible to re-electroporate subsets of cells on the array to allow for multiple gene combinations. In essence, this invention allows for the creation of cell arrays and would be analogous to gene arrays, which have been so important in recent advances in biotechnology, such as the human genome project.

Culture substrates, cell arrays, automatic chemical dispensers and assay systems necessary for conveniently assaying multiple chemicals such as drugs and toxic agents are provided. Herein used is a substrate for a high-density cell array characterized in that it has a surface comprising an ordered array of discrete microdomains coated with a cell adhesive polymer and successively surrounded by a domain coated with a cell non-adhesive hydrophilic polymer and then a domain coated with a cell non-adhesive highly hydrophobic material. This substrate for a high-density cell array and an assay system using it can greatly reduce side effects of drugs and dramatically improve the performance of drug therapy for diseases by optimizing the type and concentration of the drug used (e.g., an anticancer agent). The present invention provides a very useful technology that can also be used for drug development, environmental impact assessment and basic life science researches.

The invention discloses a glass chip for cultivating a single cell array based on microfluidic patterning technology, and a preparation method thereof, and belongs to the field of biological micro electro mechanical systems. The glass chip adopts the structure that a layer of PHEMA hydrogel pattern 2 is arranged on a glass base 4, and divides the glass base 4 into a plurality of rectangular cell growth zones 1 to generate the single cell array. The preparation process is that graphical PHEMA processing is performed on the surface of a glass sheet through a PDMS elastic stamp. The glass chip has the benefits that the stable surface chemical modification is adopted, so that hydrogel dressings capable of resisting cell adhesion is arranged on the surface of the glass in a graphical manner to form the cell graphical surface containing cell adhesion and resisting cell adhesion so as to cultivate the single cell array; the preparation process is simple and easy to operate; when the chip is applied to cell culture, the single cell growth array is obtained, and a new tool for studying basic cell biology is provided.

A cell tray has a multi-dimensional array of cells in precise, equally spaced wells (cubicles or silos) containing medium of interest. The ordered cell array enables automated processing as well as simultaneous monitoring and analyzing of a large matrix of cells, biological fluids, chemicals and / or solid samples. The invention is an integrated device and is fabricated into substrates similar to microscope slides. The ordered array of cells in precise locations helps in parallel analysis and processing of cells simultaneously. Each cell cubicle or silo in the array is located equidistant from its nearest neighbors in an orthogonal direction. The location of each well can be precisely measured and recorded in an automated processing system. Included in the bottom of each cell well is an optional micro-lens. An array of probes provides parallel cell processing and monitoring capabilities, including microinjection and microscope analysis. The cell tray when integrated with the Precision Optical Intracellular Near Field Imaging / Spectroscopy Technology (POINT or NANOPOINT) device results in sub-wavelength high-resolution imaging with a nanosensor array capable of imaging inner regions of living cells without destroying its natural environment.