120 results about "Nanosensor" patented technology

The invention discloses a reversible background-free hydrogel nanosensor for acetone detection and an intelligent sensing platform, and relates to the technical field of biosensors. The hydrogel nanosensor consists of a hydrogel matrix and an upconversion nanoprobe, and the upconversion nanoprobe comprises upconversion nanoparticles (UCNPs), hydroxylamine sulfate and thymol blue (TB). The invention adopts hydroxylamine sulfate as a specific recognition unit of acetone to construct a reversible luminescent hydrogel probe for acetone detection, and the probe can be reused in multiple detections, thereby reducing the cost of a single detection. In addition, by adopting near-infrared light as an excitation light source, the autofluorescence interference of the hydrogel matrix is ​​effectively avoided, thereby improving the detection accuracy.

The invention discloses a reversible background-free hydrogel nanosensor for acetone detection and an intelligent sensing platform, and relates to the technical field of biosensing, the hydrogel nanosensor is composed of a hydrogel matrix and an up-conversion nanoprobe, the up-conversion nanoprobe comprises up-conversion nanoparticles (UCNPs), hydroxylamine sulfate and thymol blue (TB); according to the present invention, hydroxylamine sulfate is adopted as the specific recognition unit of acetone to construct the reversible luminescent hydrogel probe for acetone detection, and the probe can be repeatedly used in multiple detections so as to reduce the cost of the single detection; in addition, by adopting near-infrared light as an excitation light source, autofluorescence interference of the hydrogel matrix is effectively avoided, and the detection precision is improved.

The present invention discloses a multi-modal system for real-time plant hydration monitoring and irrigation management. The system comprises at least one acoustic sensor configured to emit controlled sound waves through plant tissues and detect corresponding vibrations and resonance frequencies using sensitive microphones or piezoelectric sensors to assess plant hydration. The system includes at least one bioelectrical sensor for monitoring bioelectrical signals. At least one nanotechnology-based sensor is configured to detect molecular changes in water content within plant cells through embedded or externally applied nanosensors. An aerial imaging device mounted on an unmanned aerial vehicle captures data related to canopy temperature, leaf color, and hydration indicators using infrared, multi-spectral, and thermal cameras. A central processing unit receives and analyzes data using predictive models, integrates the data via data fusion algorithm to generate a plant hydration profile, and controls water delivery through an irrigation management unit based on the hydration profile.

The invention discloses a multi-parameter cooperative control method for non-excavation repair of a drainage pipe network in urban updating, and relates to the technical field of municipal engineering, and the method comprises the steps: firstly constructing a quantum entanglement sensing pipe network digital twinborn model, fusing multi-source data, monitoring micro-strain in real time through a nano sensor, deploying a self-organizing intelligent node and terahertz imaging equipment, and constructing a quantum entanglement sensing pipe network digital twinborn model; multi-dimensional features are extracted, repair parameters are dynamically regulated and controlled through technologies such as magnetofluid plugging and multi-robot cooperation based on digital twinning virtual simulation, finally, the repair effect is evaluated and the scheme is optimized in combination with cognitive calculation and microbial self-healing technologies, and full-process intelligent control is achieved. The intelligent level of non-excavation repair of the drainage pipe network is improved, the problem that traditional monitoring is one-sided is solved through high-precision data fusion, precise construction control is achieved through cooperation of magnetofluid leaking stoppage and the robot, durability is enhanced through the microorganism self-healing technology, repair quality and efficiency are improved, and urban updating and sustainable development are promoted.

The invention discloses a method for dynamically monitoring, regulating and controlling crop growth environment data based on nano sensing, and relates to the technical field of crop environment monitoring and management. Crop growth environment data and leaf dip angle data are synchronously acquired through a deployed nano sensing network; constructing an environment variation vector reflecting environment mutation intensity based on the environment data, and calculating to obtain a comprehensive risk value in combination with the blade attitude anomaly characteristic value processed by the trigonometric function; and generating a spatial risk distribution matrix reflecting continuous distribution of a risk space by using an inverse distance weighted interpolation method. By analyzing the characteristics of the matrix, transient interference caused by gust and the like and continuous stress caused by sudden change of the environment are intelligently distinguished, and two differentiated response management strategies of inhibitory regulation and control and hierarchical regulation and control are respectively triggered. The early, accurate and spatially distinguished perception and preventive intervention of the risk of the flash seedlings in the facility are realized, and the problems of high false alarm rate, response lag and extensive regulation and control of a traditional method are effectively solved.

The invention provides a high-precision material defect detection method based on a nano sensor, and relates to the technical field of material defect detection.The method comprises the steps that S1, the nano sensor is provided and detects tiny defects on the surface or inside of a material, S2, the nano sensor is installed on the surface or inside of the material to be detected, and S3, the material to be detected is detected; and the material is monitored in real time through a sensor, and signals of potential defects in the material are obtained. According to the high-precision material defect detection method based on the nano sensor, the nano sensor and an advanced artificial intelligence algorithm are combined, so that high-precision identification of tiny defects in a material is realized. Defect type classification is carried out by using a random forest algorithm, and the image data and the signal feature data are subjected to conjoint analysis, so that accurate classification and positioning of multiple defect types are ensured.

The present invention relates to the technical field of hydrogen leak detection in hydrogen refueling stations, and provides a hydrogen leak detection device and method that integrates multiple technologies. The technical solution includes: an infrared thermal imager for preliminary monitoring of potential leak hotspots; a laser spectrometer for high-precision leak positioning; a nanosensor that continuously monitors hydrogen concentration and sends out an alarm signal; an acoustic sensor array that determines the precise location of the leak source; a gas fluorescence imaging device that assists in locating the leak source; a mass flowmeter that quickly identifies flow anomalies to determine whether there is a hydrogen leak; and an alarm and control system that receives alarm signals and performs corresponding control operations. Through the coordinated work of these devices, the safety and reliability of the hydrogen refueling station can be effectively improved. The present invention realizes real-time monitoring and rapid response to hydrogen leaks, significantly reduces the risk of leaks, and ensures the safe operation of the hydrogen refueling station.

The invention discloses a carbon nanosensor with a gradient pore structure and a preparation method and application thereof. The carbon nanosensor comprises a sensing base layer, a sensing layer and a flexible electrode, the sensing layer is located between the sensing base layer and the flexible electrode; the sensing layer is prepared by mixing at least two of a zero-dimensional conductive nano-material, a one-dimensional conductive nano-material, a two-dimensional conductive nano-material and a three-dimensional conductive nano-material with a surfactant; the sensing base layer is prepared from a high-molecular polymer, and the pore structure of the sensing base layer is sequentially increased along the direction far away from the sensing layer to form a gradually-changed gradient pore structure. According to the invention, the defects of signal response lag, large measurement error, long-term performance degradation and the like of a traditional sensor can be overcome, high-sensitivity, high-precision and high-stability collaborative sensing of the material structure state under complex working conditions is realized, and a breakthrough technical scheme is provided for the field of structure health monitoring.

The invention belongs to the field of nano sensors, and discloses an electrochemical sensor based on a porous silicon-platinum nanoflower-DNA composite material and a preparation method and application thereof. A porous silicon layer is prepared on a polished surface of a silicon wafer, then platinum nanoflowers and a T-rich sulfhydrylation DNA probe are modified, a metal film is plated on the other surface of the silicon wafer, and the electrochemical sensor is prepared and applied to melamine detection. The DNA probe can adsorb melamine on the surface of a material through a hydrogen bond and generate current response linearly related to the concentration of the melamine; hydroxyl on the surface of the porous silicon can enhance the adsorption effect on melamine; the platinum nanomaterial with the flower-shaped structure can enhance the sensitivity of the sensor; in addition, negative charges carried on the surface of the porous silicon and common coexisting substances of melamine have an electrostatic repulsion effect, so that the sensor has good selectivity. The high-performance nano sensor provides a novel method with practicability for on-site rapid detection of melamine.

The invention relates to the technical field of bridge cable health monitoring, in particular to an intelligent evaluation method and system for the bearing capacity of a bridge cable in a corrosion environment, and the method achieves the real-time monitoring of a microcrack and a stress state through the deployment of a nano sensor and a strain sensor on the surface of the cable. After data acquisition, noise is eliminated and missing data is complemented through a data preprocessing module, so that the accuracy of the data is ensured; then, the system performs coupling analysis on the microcrack data and the stress data by using a multi-dimensional data fusion algorithm to generate a stress distribution diagram, and evaluates the influence of microcrack propagation on the bearing capacity; and based on the corrosion fatigue model, predicting the change trend of the bearing capacity of the inhaul cable in a period of time in the future, and generating a detailed evaluation report. When the bearing capacity is reduced to a safety threshold value, early warning is automatically triggered, and maintenance suggestions are provided, so that effective support is provided for safety management of the bridge; according to the method, the safety and the operation efficiency of the bridge are improved, and the accident risk caused by structural failure is reduced.

The invention provides a nano sensor monitoring method for pressure of a fiber prepreg laying layer, and relates to the technical field of composite material manufacturing process control. According to the method for monitoring the pressure between the composite material fiber prepreg layers, the flexible pressure sensor is provided with a multi-dimensional nano material with high elasticity modulus, and the monitoring unit is prepared according to the size and shape of the composite material fiber prepreg; a monitoring unit is used for collecting pressure data of each monitoring position between the composite fiber prepreg layers and calculating a pressure value between the composite fiber prepreg layers; according to the pressure value between the composite material fiber prepreg layers, the pressure distribution between the composite material fiber prepreg layers is monitored, the dynamic change of a pressure field is accurately identified, abnormal point data is timely captured, whether defects are caused in the automatic layer loading process or not is determined, and the purpose of guiding process optimization is achieved.

The invention discloses a fluorescent nanosensor based on the Strecker-like method for synthesizing isoindole and combining cadmium telluride quantum dots and its application, which is a composite system composed of 2,3-naphthalene dicarboxaldehyde and potassium sulfite as probe solutions and combined with cadmium telluride quantum dots. Cadmium telluride quantum dots are used as a fluorescence source to emit bright red fluorescence, 2,3-naphthalene dicarboxaldehyde and potassium sulfite are open-type probe solutions, and the addition of carbamate pesticides interacts with 2,3-naphthalene dicarboxaldehyde and potassium sulfite to synthesize an isoindole derivative to emit green fluorescence, thereby realizing the detection of carbamate pesticides based on the ratio fluorescence principle. The nanosensor relies on the fluorescence open-type response detection signal, presenting a significant change from bright red to green fluorescence, having a good visualization effect, and having high sensitivity, good selectivity, strong anti-interference ability, and low detection limit.

This invention belongs to the field of gas sensor technology and discloses a method for preparing europium ferrite nanosensors and their applications. This method utilizes electrospinning technology combined with a specific low-temperature confined-domain calcination process (550℃~700℃) to prepare EuFeO3 nanotubes with a hollow structure and rough surface by taking advantage of the thermal decomposition kinetics differences of poly(p-v) magnets (PVP). This process effectively suppresses excessive repair of lattice oxygen while forming a unique tubular morphology, effectively "freezing" oxygen vacancies (O2) accounting for up to 34.44% on the material surface in situ. v This invention addresses the shortcomings of traditional solid fiber materials by constructing a sensor using a grinding, dispersion, and coating process. Utilizing the dual reactive surfaces of hollow nanotubes and high-concentration oxygen vacancy active sites, it achieves highly sensitive and selective detection of low-concentration ethylene gas released during fruit ripening. This invention solves the problems of small specific surface area and insufficient active sites in traditional solid fiber materials.

The present invention relates to the technical field of composite material health monitoring, and particularly to a method and system for health monitoring of composite material preforms based on carbon-based nanosensors. In the present invention, carbon-based nanosensors are implanted into the composite material preforms without significantly affecting the performance of the preforms themselves. The implantation method of the sensors is unified with the forming process of the composite material preforms. The use of these sensors does not increase the difficulty of the forming process, realizes the internal health monitoring of the composite material preforms, and avoids problems such as sensor damage that may be caused by the implantation method of other sensors as foreign objects, greatly improving the success rate of sensor implantation.

The application discloses a digital image quantitative dual-mode detection system and method based on a cascade enhanced nano-enzyme, which comprises a probe prepared from a membrane nano-enzyme material, an immunochromatography test strip and a signal automatic analysis system, the nano-enzyme material takes molybdenum disulfide nanosheets as a substrate, sequentially loads large-diameter platinum-iridium alloy particles and small-diameter platinum-iridium alloy particles through polyethylene imine-mediated electrostatic self-assembly, and forms a high-density catalytic active site array. The probe is coupled with specific antibodies on the surface of the nano-enzyme material. Based on the inherent light absorption and peroxidase-like activity of the probe, a dual-mode detection strategy of physical color development-catalytic enhancement is established, high-concentration sample visual preliminary screening and low-concentration sample ultra-sensitive quantitative detection are realized, and combined with an adaptive cross-peak colorimetric quantitative algorithm, individual visual perception differences can be effectively eliminated, and on-site rapid quantitative screening of community-acquired pneumonia pathogens such as streptococcus pneumoniae and legionella pneumophila can be realized.

The invention discloses an electric vehicle lithium battery box fire prevention and control system and a fire extinguishing method thereof, and the system comprises the steps: S1, a multi-dimensional composite monitoring network which comprises an integrated nano sensor array, a terahertz imager and a quantum dot fluorescence sensor; s2, the intelligent decision and control center establishes a fire risk assessment model of a convolutional neural network CNN and a long-short term memory network LSTM; the self-adaptive fire extinguishing execution module is composed of a plurality of independent micro fire extinguishing units; s4, a hybrid energy system composed of an energy and communication redundancy guarantee module, an equipped super capacitor and a micro fuel cell; through an innovative multi-dimensional composite monitoring network, a plurality of advanced technologies such as a nano sensor, a terahertz imager and a quantum dot fluorescence sensor are fused, and all-directional and high-sensitivity monitoring of fire hazards in the battery box is realized. According to the invention, extremely weak abnormal signals can be captured in the extremely early stage of a fire, so that the accuracy and timeliness of fire early warning are greatly improved.

The invention provides a multiple access scheme in a molecular communication network and a detection method, which are used for relieving a near-far effect and ensuring communication fairness of sensors in different distances by constructing the network, setting time offset according to a transmission distance, determining emission delay by combining channel impulse response peak time and adjusting molecular emission quantity according to channel impulse response. The molecule consumption and energy consumption are reduced; based on the MMSE principle, an optimization equation is constructed, an optimal weight vector is solved, a judgment variable is obtained, bit information is precisely restored, interference is effectively suppressed, the bit error rate is reduced, the reliability and efficiency of nano sensor network communication are greatly improved on the whole, and the problem of nano sensor network communication is efficiently solved.

The invention relates to the technical field of luminescent materials, in particular to a nano material and a preparation method and application thereof. The invention provides a nanometer material, the nanometer material is NaYbF4: Er (at) NaYF4 (at) Cy7.5 (at) DPPC, the nanometer material comprises a core-shell structure with the component of NaYbF4: Er (at) NaYF4, and the core-shell structure is modified with DPPC and compounded with Cy7.5. When the nano material provided by the invention is used for an optical nano indicator or sensor for detecting the membrane potential under an NIR-IIb window, the sensor generates emitted light (1500-1700nm) under the NIR-IIb window under the excitation of 808nm near-infrared incident light. By utilizing the nano sensor, the membrane potential change of cells marked by the sensor can be visually detected by means of optical imaging.

The present disclosure provides a colorimetric nanosensor for detecting trace-levels of Hg2+ in environmental water, which is composed of Fe7S8 nanosheets, glutathione or its aqueous solution, 3,3′,5,5′-tetramethylbenzidine colorimetric solution, H2O2 aqueous solution, and NaAc-Hac buffer. The preparation method of Fe7S8 nanosheets is as follows: FeCl2·4H2O and CH4N2S are dissolved in ethylene glycol in a mass ratio of (2˜3):1, heated at 200° C. for 10˜15 hours, cooled, centrifuged, washed, and dried to obtain. The colorimetric nanosensor of the present disclosure can quickly and sensitively determine the trace-levels Hg2+ in environmental water. The colorimetric nanosensor of the present disclosure does not rely on large detection instruments, and the nanozymes used is low-cost, stable, and recyclable. It has important practical significance for accurate, fast, and low-cost detection of trace-levels of Hg2+ in environmental water.

The invention discloses a rare earth nanoprobe, a preparation method and application thereof, and belongs to the technical field of biomedical detection.According to the technology, the rare earth nanoprobe with the high fluorescence quantum yield is synthesized, specific recognition of tumor markers is achieved through the aptamer technology, and the detection sensitivity and selectivity are remarkably improved. The fluorescence signal amplification effect of the rare earth nanoprobe and the modern fluorescence detection technology are innovatively combined, so that simultaneous detection of multiple tumor markers such as carcino-embryonic antigen (CEA), prostate specific antigen (PSA) and alpha fetoprotein (AFP) becomes possible. According to the method, the detection limit reaches the pg / mL level, the operation is simple and convenient, the cost is low, and the feasibility of clinical application is greatly improved. Besides, through multiple detection design, the detection efficiency and accuracy are further improved, an effective tool is provided for early diagnosis, curative effect monitoring and prognosis evaluation of tumors, and wide clinical application prospects and market potential are shown.

The invention discloses a preparation method of brassinolide derived carbon quantum dots and application of the brassinolide derived carbon quantum dots in plant hormone receptor detection. According to the method, BRs is used as a precursor, CQDs with a BRs semi-reserved structure are prepared through co-carbonization with citric acid, and the nano material with the particle size of 3.1 + / -0.5 nm and the surface rich in hydroxyl / keto is obtained after dialysis and purification. When the CQDs are combined with the BRs receptors, the fluorescence intensity of the CQDs is linearly enhanced along with the concentration of the receptors, the nano sensor constructed based on the CQDs can realize rapid quantitative detection of the BRs receptors in rice leaves, the linear range is 0.01-4.0 mg / mL, the detection limit is as low as 0.003 mg / mL, and the sensitivity is improved by 10 times compared with that of ELISA (Enzyme-Linked Immunosorbent Assay). By detecting the receptor expression quantity of a wild type and BRs receptor overexpression strain in a germination period, a heading period and a mature period, it is verified that the result of the strain is highly consistent with that of Western Blot, and the detection time is shortened to 30 minutes. The method does not need targeted modification, has the advantages of high specificity, interference resistance and low cost, and provides an efficient detection tool for rice molecular breeding and stress resistance regulation.

The present invention generally relates, in some aspects, to articles and methods relating to nanosensors for determination of molecules and other features, e.g., via surface plasmonic resonance, electric resonance, magnetic resonance, color changes, or the like. These articles and methods may be used, for example, for sample detection. The articles described in some aspects of the invention include a microwell array and a nanosensor array. In some embodiments, the nanosensor arrays may utilize nanoparticles positioned on nanostructures that are able to interact with a sample suspected of containing an analyte, such as a single cell. The interaction between nanoparticles and a sample can be detected by a change in applied energy, such as altered electromagnetic radiation caused by surface plasmonic resonance of incident visible light, and / or other types of resonance. Electromagnetic radiation may be applied to a microwell array and nanosensor, and the applied electromagnetic radiation may be altered as a nanosensor interacts with a sample suspected of containing an analyte. In addition, in some embodiments, the nanosensor arrays may utilize nanostructures and the binding of analytes may be determined based on optical or color changes.

The present invention discloses a zinc nitride quantum dot and its application as a fluorescent nanosensor in the detection of Cu<supgt;2+< / supgt> and Mn<supgt;2+< / supgt>. The complexation of blue fluorescent quantum dots containing rich functional groups with metal ions Cu<supgt;2+< / supgt> and Mn<supgt;2+< / supgt> results in a strong quenching of the blue fluorescence, thereby realizing the change of the fluorescence color of this probe from blue to colorless for the detection of Cu<supgt;2+< / supgt> and Mn<supgt;2+< / supgt>. This method has good visualization effects, high selectivity, high sensitivity, and low detection limits. In addition, the chemicals and technologies used have less economic harm, stable materials, and high quantum yields. It has been successfully coated on paper strips and can be used as a mobile detector for on-site local sensing of heavy metal ions.

This invention relates to a three-emission ratio fluorescence nanosensor for detecting tetracycline and its preparation method. The sensor uses a carbon dot-gold cluster nanocomposite material as the fluorescence signal. The synthesis steps and detection process of the carbon dot-gold cluster nanocomposite material in this invention are simple, convenient, and rapid, exhibiting rich fluorescence color changes. It does not require the assistance of complexes or coupling reagents. By constructing a three-emission ratio fluorescence sensor, the goal of highly sensitive and selective detection of tetracycline can be achieved.

The present invention belongs to the field of biological analysis, and provides a methylation-driven dual-color luminescent RNA nanosensor for label-free and ultra-sensitive detection of multiple DNA methyltransferases. The dual-color luminescent RNA nanosensor is constructed by integrating cascade nucleic acid amplification and dual-color luminescent RNA aptamers, thereby realizing label-free and ultra-sensitive detection of multiple DNA methyltransferases. The ultra-high specificity of MDREs for methylated DNA, efficient double-loop cascade amplification, and high signal-to-noise ratio of luminescent RNA aptamer-fluorophore pairs are utilized. Compared with the experiments reported for DNA MTase, this nanosensor has obvious advantages: (1) ensuring accurate quantification of low-abundance DNA MTases; (2) significantly improving amplification efficiency; (3) realizing label-free and simultaneous detection of multiple DNA MTases at a high signal-to-noise ratio; and detecting in a label-free manner, which is convenient to operate and reduces costs.

The preparation method comprises the following steps: firstly, preparing ytterbium and thulium doped sodium yttrium tetrafluoride nanoparticles, then preparing a PD probe, and incubating the ytterbium and thulium doped sodium yttrium tetrafluoride nanoparticles and the PD probe in a poly-L-lysine aqueous solution to form a light-operated unit; mixing DPPC, DPPE and CHOL, adding an aptamer buffer solution containing a ferrocene label, adding a buffer solution containing a light-operated unit, and incubating to obtain the nano sensor. According to the structure and principle design, accurate dynamic monitoring of adenosine is achieved under the condition that tissue is not damaged, and an effective tool is provided for research of depression and related inflammations.

The invention relates to a bearing-energy storage-structure health monitoring integrated composite material and application, and belongs to the field of functional composite materials. Structural health monitoring is realized through a quantum tunneling effect of the nano sensor under ultrasonic excitation, and mutual conversion between electric energy and chemical energy is completed through electronic physical adsorption or chemical reaction so as to realize energy storage capacity. Good aerodynamic configuration of the aircraft can be guaranteed, mechanical property requirements of structural load bearing can be met, structural weight and size can be reduced, electric energy can be stored to supply power to the aircraft locally, and structural health monitoring capability of the embedded nano sensor is matched, so that reliability of the aircraft is improved. The composite material intelligent skin structure integrating bearing, energy storage and health monitoring is achieved.

The invention discloses a colorimetric sensing array based on hierarchical pore nano-enzyme as well as a construction method and application of the colorimetric sensing array, and belongs to the technical field of analysis and detection. According to the array, two cerium-based MOF nano-enzymes, namely micropore UiO-66 and hierarchical pore HP-UiO-66, are respectively combined with TMB (tetramethylbenzidine) and ABTS (2, 2, 6, 6-tetramethylbenzidine) chromogenic substrates to form a four-channel sensing system. The method comprises the following steps: synthesizing hierarchical pore HP-UiO-66 by a template method; collecting the absorbance of each channel under the characteristic wavelength by using an array, and generating a specific fingerprint spectrum of the to-be-detected object; by combining mode recognition technologies such as linear discriminant analysis and the like, rapid and synchronous distinguishing and quantitative detection of at least eight antioxidants with similar structures such as glycyrrhizic acid and caffeic acid in the traditional Chinese medicine decoction are realized. According to the method, the catalysis and mass transfer efficiency is improved by regulating and controlling the pore structure of the nano-enzyme, the problem of multi-target synchronous recognition in a complex matrix is solved by utilizing array cross response, and the method has the advantages of simplicity and convenience in operation, high flux and good accuracy.

The present invention relates to a bio-thiol responsive AIE fluorescent probe and its preparation method and application. Based on the competitive regulation of AuNCs in ZIF-8, the present invention develops a unique "turn-on-turn-off-turn-on" method for highly sensitive and rapid sensing of bio-thiols. Different from traditional AIE, the aggregation-induced emission is regulated and quenched by the pre-embedded competitive factor of Hg<supgt;2+< / supgt; for the development of visual sensing nanoprobes. In the presence of bio-thiols, the mediator of Hg<supgt;2+< / supgt; competes with AuNCs based on the formation of stronger Hg<supgt;2+< / supgt>-S bonds, resulting in the recovery and enhancement of fluorescence. The designed nanoprobe can successfully respond to trace bio-thiols in serum samples. Importantly, compared with free AuNCs, its solid stability retains great potential for the sensing of bio-thiols, which can highly promote the progress of disease diagnosis.

The application belongs to the technical field of analytical chemistry, and particularly relates to a preparation method and application of a dual-mode sensor based on ZnIn2S4 / Ag4V2O7 / PTCA heterojunction and CuFe2O4-AuNPs nanosensor. The preparation steps of the sensor are as follows: (1) ITO substrate pretreatment; (2) heterojunction modification layer construction; (3) antibody immobilization interface engineering; (4) biological recognition interface optimization; (5) antigen-antibody specific reaction; (6) signal probe assembly; (7) detection process. The CEA antigen solution is a standard or an actual biological sample.