Nano-sensor for detecting antibiotics and preparation method and application of nano-sensor

A nano-sensor and antibiotic technology, applied in the fields of biochemical analysis and food safety testing, can solve the problems of inability to specifically indicate the type of antibiotics, complicated operation, etc., and achieve the effect of facilitating qualitative and quantitative judgment, solving quantitative difficulties, and shortening test time.

Active Publication Date: 2015-04-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is complicated to operate and

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  • Nano-sensor for detecting antibiotics and preparation method and application of nano-sensor

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Embodiment 1

[0024] Preparation of nanosensors for chloramphenicol and kanamycin detection and applications of the nanosensors:

[0025] (1) Preparation of gold nanoparticles with a diameter of 13nm: weigh 0.04726g HAuCl 4 Dissolve in a round-bottomed flask with 100mL of ultrapure water, put a magnetic stirrer, then connect a condenser tube and a stopper to the two ports of the two-necked flask, turn on the condensate, turn on the magnetic stirrer and start heating. In addition, 1.3693 g of trisodium citrate was dissolved in ultrapure water, and configured into a 120 mL solution to obtain a 38.8 mM trisodium citrate solution. When the reaction solution boils and the condensed water starts to flow back at a rate of 1 drop per second, remove the stopper, quickly add 12mL of 38.8mM trisodium citrate, and reinstall the stopper. At this time, the color of the solution will gradually change from light yellow to dark red, and then continue to heat and reflux for 15 minutes after turning dark red...

Embodiment 2

[0039] Preparation of nanosensor for neomycin detection and application of the nanosensor:

[0040] (1) Preparation of gold nanoparticles with a diameter of 5 nm: 70 mL of distilled water and 10 mL of chloroauric acid with a mass fraction of 0.1% were added to a 250 mL three-necked flask, and heated to boiling under vigorous stirring. Then, quickly add 4 mL of freshly prepared mixed solution with a mass fraction of 1% sodium citrate and 5 mL of a mixed solution with a mass fraction of 1% tannic acid to the above-mentioned boiling solution, and after reacting for 10 minutes, continue stirring to cool the solution to room temperature, Store at ℃.

[0041] (2) Modification of sulfhydryl ssDNA onto the surface of AuNPs: the present invention selects nucleic acid aptamers with high affinity and high specificity for neomycin and labeled with fluorescent molecules as recognition molecules, and their sequences are respectively:

[0042] Aptamer E specifically recognizing neomycin: 5′...

Embodiment 3

[0047] Preparation of nanosensors for detection of ampicillin and streptomycin and application of the nanosensors:

[0048] (1) Preparation of gold nanoparticles with a diameter of 30 nm: 100 mL of chloroauric acid with a mass fraction of 0.01% was added to a 250 mL three-necked flask, and heated to boiling under vigorous stirring. Then, quickly add 1 mL of freshly prepared 1% sodium citrate solution into the boiling solution, react for 15 minutes, continue stirring to cool the solution to room temperature, and store at 4°C.

[0049] (2) Modification of sulfhydryl ssDNA onto the surface of AuNPs: the present invention selects nucleic acid aptamers with high affinity and high specificity for streptomycin and ampicillin and labeled with fluorescent molecules as recognition molecules, and their sequences are respectively for:

[0050] Aptamer C that specifically recognizes streptomycin: 5′-SH-TAGGGAATTCGTCGACGGATCC-GGGGTCTGGTGTTCTGCTTTGTTCTGTCGGGTCGT-CTGCAGGTCGACGCATGCGCCG-Rox-3...

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Abstract

The invention discloses a nano-sensor for detecting antibiotics. The nano-sensor comprises gold nano-particles, wherein the surfaces of the gold nano-particles are modified with at least one kind of aptamers which can be specifically bonded with specific antibiotics through an Au-S bond, and the aptamers are labeled with a fluorescent dye. The invention aims to provide the nano-sensor capable of quickly, accurately, qualitatively and quantitatively determining one kind of antibiotics or synchronously testing the multiple kinds of antibiotics, and also provide a preparation method and application of the nano-sensor.

Description

technical field [0001] The invention relates to the fields of biochemical analysis and food safety detection, and more specifically relates to a nanosensor for antibiotic detection, a preparation method and application thereof. Background technique [0002] With the improvement of people's quality of life, food safety issues are more and more concerned by consumers, especially the problem of antibiotic residues in food has become the focus of consumers' attention. After eating meat, eggs, milk, etc. with antibiotic residues, the antibiotics will accumulate in the human body, causing people to develop resistance to antibiotics, causing various tissue and organ lesions, and even cancer. On food safety, a hot issue of global concern, how to quickly and accurately detect food safety has become a top priority. [0003] Both kanamycin and neomycin are broad-spectrum aminoglycoside antibiotics that have excellent inhibitory effects on Gram-negative bacteria. But the absorption to...

Claims

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Application Information

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IPC IPC(8): G01N33/553G01N21/64
CPCG01N21/6428G01N33/553G01N2021/6432
Inventor 易长青张亚丽张恒宋嵘蒋乐伦
Owner SUN YAT SEN UNIV
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