95 results about "Antibiotic testing" patented technology

The present invention provides a platform technology for testing, screening, selecting and evaluating antibiotics by using genetically engineered strains with identified, individual or combined, resistance mechanisms, prepared from fully susceptible clinical isolates. This antibiotic testing and screening system of the present invention can efficiently and effectively evaluate antibiotics against specified resistance mechanisms in vitro and in vivo, and is suitable on the novel antibiotic development in against multidrug-resistant bacteria.

The invention discloses a nano-sensor for detecting antibiotics. The nano-sensor comprises gold nano-particles, wherein the surfaces of the gold nano-particles are modified with at least one kind of aptamers which can be specifically bonded with specific antibiotics through an Au-S bond, and the aptamers are labeled with a fluorescent dye. The invention aims to provide the nano-sensor capable of quickly, accurately, qualitatively and quantitatively determining one kind of antibiotics or synchronously testing the multiple kinds of antibiotics, and also provide a preparation method and application of the nano-sensor.

The invention belongs to the technical field of detection of enrichment of remained antibiotics in a water body environment, and particularly relates to a synchronous detection method of tetracyclines, fluoroquinolones and sulfonamide antibiotics remained in the water body. The detection method comprises the following steps: (1) performing water sample pretreatment; (2) performing small-column enrichment of target antibiotics by using solid-phase extraction; (3) detecting on a machine by using high performance liquid chromatography and tandem mass spectrum. According to the method, once the sample feeding is carried out at one time, multiple antibiotics in the water body can be detected at the same time, the time consumption of a detection process is reduced, the detection accuracy and sensitivity are increased, the detection limit is low, and the condition of the trace amount of remained antibiotics in the water body environment can be detected; meanwhile, the detection method is convenient to operate and low in environment toxicity and is low in using amount of organic reagents.

The invention relates to an antibiotic detection method, and in particular, relates to a method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography; the method comprises the steps: pretreating a livestock and poultry manure sample, to obtain a to-be-detected liquid; performing analyte separation of the to-be-detected liquid by a high performance liquid chromatograph, and calculating peak areas corresponding to separated residual antibiotics; and quantitatively calculating according to a standard curve, to obtain the residual content. The method is applied in detection of 11 kinds of residual antibiotics in the livestock and poultry manure; compared with a high performance liquid chromatography-mass spectrometry coupled technique, the method has the advantages of lower detection cost and high detection efficiency, can simultaneously separate 11 kinds of antibiotics, has analytes without interference of major interferents, and basically does not affect qualitative and quantitative analysis of the analytes.

The invention provides a method for simultaneously detecting 11 kinds of antibiotics in soil. The method utilizes an UPLC-MS/MS method to detect antibiotics and utilizes a standard addition method to determine a recovery rate of the detection method. The method mainly comprises extraction of antibiotics in soil, extraction and enrichment of the antibiotics and detection of the antibiotics. The method comprises (1) sample collection and preservation: collecting surface soil with thickness of 0-20cm, keeping a low temperature through an ice bag, carrying out dark preservation, cool-drying the collected soil sample, grinding the soil sample, sieving the soil sample through a sieve of 40 meshes, weighing 1g of the soil and carrying out antibiotic extraction, (2) extracting and purifying the extracted antibiotics in a form of SAX-HLB small column series connection, eluting the extract through 10ml of methanol, blowing nitrogen until complete drying, carrying out enrichment, carrying out volume metering through the pure methanol until the volume is 1 ml and feeding the solution into a small bottle, and (3) UPLC/MS/MS detection. The extraction solution used by the method comprises acetonitrile and a phosphate buffer solution with pH of 3 according to a ratio of 7: 3 and has the volume of 10 ml, the extraction process is carried out three times, the supernatant is merged and is subjected to SAX and HLB small column solid phase extraction, high purity nitrogen is blown to completely dry the extract, methanol is used until the solution volume is 1ml, and the solution is detected through the UPLC-MS/MS method.

The invention relates to an antibiotic detection method based on a molecular imprinting-Raman spectrum, and belongs to the technical field of food detection. Antibiotics are used as template molecules; a composite nanometer material (MIP-PNM-Au) composed of an antibiotic molecular imprinted PNIPAM temperature-sensitive gel film and nanogold is provided; and a Raman enhancement effect of the composite material is utilized, so that the enrichment and the ultra-sensitive Raman spectrum detection of the antibiotics are realized. According to the method, a specific recognition function of a molecular imprinting technology (MIP), an intelligent response characteristic of gel and an SERS property of the nanogold are combined. The MIP-PNM-Au composite material is improved in selective enrichment effect through the MIP, and parameters such as the density, the interval and the like of nanoparticles are adjusted by means of the temperature-sensitive property of the gel, so that the surface plasmaresonance and the electromagnetic field intensity are tuned, dynamic generation of "hot spots" is realized, and the Raman enhancement effect is optimized.

The invention relates to an antibiotic detection method. The method concretely comprises the following steps: (1) carrying out pretreatment for vegetable samples in order to obtain an antibiotic extract; (2) carrying out extraction for the antibiotic extract in order to obtain an antibiotic eluate; (3) evaporating the antibiotic eluate to dryness by a rotary evaporator, carrying out volumetric flask preparation with an initial mobile phase, and carrying out filtering with a syringe filter in order to obtain an antibiotic liquid to be measured; (4) carrying out detection for the antibiotic liquid to be measured by optimized high performance liquid chromatography-tandem mass spectrometry in order to detect antibiotics to be measured. The method for detecting 11 kinds of antibiotic residues in vegetable samples has the advantages of high recovery rate and low detection limit, and overcomes the disadvantages and insufficient that antibiotic detection methods in the prior art have high cost, few antibiotic detection kinds, and high detection limit; a base for assessing antibiotic environmental behaviors and risks is established.

The invention belongs to the field of antibiotic detection, and relates to a method for analyzing and determining four sulfonamide antibiotics in a water sample. A negative pressure suction filtrationmembrane solid phase extraction technology is utilized, and a metal organic framework material NH2-MIL-101(FE) membrane solid phase extraction-high performance liquid chromatography method is adoptedto determine four antibiotics such as sulfadiazine, sulfamethazine, sulfachloropyridazine and sulfamethoxazole in an environmental water sample. The method is simple, convenient, rapid and sensitiveto operate, and has the advantages of low detection limit, accuracy and good reproducibility; and moreover, the raw materials are cheap and easy to obtain, the material preparation process is simple,the reaction conditions are mild, the application environment is friendly, and the market prospect is wide.

The invention provides a luminescent metal organic framework material for detecting antibiotic contaminants in water. The luminescent metal organic framework material is prepared from an indium salt and 1,4-naphthyl bis(benzoic acid) by adopting a solvothermal method. The luminescent metal organic framework material has a chemical sensing function and can be used for detecting nidazole-class antibiotic contaminants in water. The luminescent metal organic framework material is simple in preparation technology, high in material purity, and good in stability; and the synthesized luminescent metalorganic framework material has the advantages of rapidness, simplicity, convenience, high sensitivity and the like in the detection of nidazole-class antibiotics, and has broad application values.

The invention discloses a near infrared antibiotic fluorescent probe detecting agent and a preparing method and application thereof. The near infrared antibiotic fluorescent probe detecting agent comprises a fluorescent nanometer material. The excitation spectrum of the fluorescent nanometer material and the absorption spectrum of antibiotics are at least partly overlapped so that the excitation wavelength of the fluorescent nanometer material can be absorbed by the antibiotics and the fluorescence of the fluorescent nanometer material can be quenched. The fluorescent nanometer probe agent which is high in selectivity, high in detection sensitivity, simple and feasible and can be used for detecting tetracycline and carbostyril antibiotics is established according to the principle of the internal filtering effect formed between near infrared carbon quantum dots and other fluorescent nanometer materials and the antibiotics, the selectivity of the fluorescent nanometer probe is improved,the technical problem that an existing antibiotic detecting probe is not enough in sensitivity and complicated in detecting process can be solved, the detecting agent has a remarkable effect when usedfor detecting actual samples, and the detecting agent can be easily designed into a sensor for detecting the antibiotics in the actual samples on site.

The invention discloses a graphene-metamaterial absorber comprising graphene and a metamaterial absorber body. The metamaterial absorber has an array structure formed by a plurality of single absorbers. Each single absorber includes a bottom layer, an intermediate medium and a top layer; and the bottom layer is made of aluminum, the intermediate medium is made of high-resistance silicon, and the top layer is made of gold. In addition, the invention also includes application of the graphene-metamaterial absorber in antibiotic detection. According to the invention, bottlenecks of complex samplepretreatment process, long analysis period, high organic solvent consumption, higher requirement on professional ability of operators, inconvenience in popularization and use of at the basic level andthe like in the traditional antibiotic detection method are broken through. With the graphene-metamaterial absorber as a carrier, the rapid and nondestructive antibiotic concentration characterization can be realized.

A process of concentration by precipitation of PrP^sc for detecting or diagnosing PrP^sc, wherein a tissue or biological fluid stemming from or obtained from an animal or human organism is brought together with an antibiotic from the family of aminoglycosides, preferably streptomycin or one of its derivatives. The use of such an antibiotic for eliminating PrP^sc from a tissue or biological fluid and a kit for diagnosing pathologies associated with the presence of PrP^sc is also disclosed.

The invention discloses a detection device and a detection method of antibiotics based on a micro-fluidic chip. The device comprises a substrate, a separation channel, a buffer solution hole, two sample storage holes and a detection hole are formed on the substrate, one end of the separation channel respectively communicates with the buffer solution hole and the two sample storage holes through micro-channels, the other end of the separation channel communicates with the detection hole, the detection hole communicates with a waste liquid hole through a micro-channel, the position, close to the detection hole, of the substrate is provided with a micro-groove, an excitation light source and a fluorescence detector are arranged in the micro-groove, a sample injection electric field is formed between the two sample storage holes, a separation electric field is formed between the buffer solution hole and the detection hole, one end of the buffer solution hole is a high voltage end, and one end of the detection hole is a low voltage end. The detection device overcomes the shortcomings of large sample demand, narrow application field and high test cost of the traditional antibiotic detection devices, and realizes online qualitative and quantitative detection of the antibiotics.

The invention provides a monoclonal antibody for detecting aminoglycoside antibiotics and a kit thereof. A hybridoma cell strain Pami-SP20 with the preservation number being CCTCCC201135 is acquired; andaccording to an aminoglycoside antibiotics residue quick-detection method established by a monoclonal antibody excreted by the strain, the monoclonal antibody excreted by the monoclonal hybridoma cell strain can be directly applied in aminoglycoside antibiotics remained ELISA (Enzyme-linked Immuno Sorbent Assay) quick-detection reagent preparation and detection of an acquired antibody remained by the aminoglycoside antibiotics through a double-antibody sandwich ELISA method. The aminoglycoside antibiotics detection kit comprises a detection plate wrapped by a streptomycin-BSA (Bovine Serum Albumin) antigen, the monoclonal antibody marked with HRP (Horse Radish Peroxidase), and ELISA reaction liquid. The monoclonal antibody excreted by the monoclonal hybridoma cell strainhas the advantages of strong specificity and high affinity, and provides a new manner for quick detection of aminoglycoside antibiotics remaining.

The invention provides a detection method of belt-lactam antibiotic residues in milk. The belt-lactam antibiotic residues in milk is detected by the following three steps of centrifugation, chemical reaction of the belt-lactam antibiotic and heavy metal and ultraviolet spectrophotometry; the sample amount and the dosage of various reagents are less in the detection process; the method has less harm on the environment and rapid speed, and the whole operation process can be finished within 30min; and the detection can be finished only in need of equipping a low-speed centrifugal machine and an ultraviolet spectrophotometer without purchasing expensive antibiotic detector.

The invention discloses a micro sensor-based antibiotic detection device and a manufacturing method thereof as well as a micro sensor-based antibiotic detection method. The micro sensor-based antibiotic detection device comprises a micro sensor and a picoammeter with a voltage output function, wherein the voltage output end of the picoammeter is connected with an electrode interface of the micro sensor; a working electrode of the micro sensor is coated with a PFSA (perfluorinated sulfonic acid) macromolecular film; and magnetic nano core-shell type microspheres are embedded into the PFSA macromolecular film. By use of the high adsorption performance of the magnetic nano core-shell type microspheres and the advantages of quick response, small sample use amount, high interference resistance and the like of the micro sensor, the micro sensor-based antibiotic detection device can realize effective, simple, quick and accurate detection for the concentration of antibiotics in a liquid phase.

The invention involves preparation method of anatase type TiO2 photo-catalytic powder making use principle of liquid phase precipitation method, it mainly includes: providing solution containing titanium, making the solution containing titanium and an acidic or alkaline solution react and purify at room temperature, said alkaline solution can be caustic soda, ammonium hydroxide and potassium hydrate, said acidic solution can be nitric acid, sulfuric acid, phosphoric acid, hydrochloric acid and boric acid. It is analyzed and displayed that by apparatus that, the titanium oxide powder prepared in the invention is anatase type TiO2, and according the photocatalysis experiments and antibiotic testing experiment results, the titanium oxide powder prepared in the invention has good photocatalysis and antibiotic performance.

The invention discloses a data automatic collection method and apparatus. The method comprises the steps of providing a data collection interface, and establishing a communication connection with an antibiotic detection instrument selected in the data collection interface by detection personnel; after the detection personnel select a general detection option in the data collection interface, obtaining identifier information of a to-be-detected sample; receiving a detection item selected in the data collection interface by the detection personnel; obtaining a detection item value of the to-be-detected sample for the detection item from the antibiotic detection instrument; and controlling the detection item of the to-be-detected sample and the corresponding detection item value to be displayed in the data collection interface. According to the method, automatic collection of antibiotic detection data is realized, and detection data of an electronic balance does not need to be manually recorded, so that the manual recording errors are avoided, the manual operation is reduced, the working efficiency is improved, an accurate data basis is provided for subsequent results, and the accuracy of the subsequent results can be improved.

The invention provides a method for determining antibiotics in water by solid-phase extraction-liquid chromatography-tandem mass spectrometry by using a special column for a large-volume water sample.The method comprises the steps of (1) activating a special column for large-volume water by using methanol and formic acid; (2) taking an appropriate amount of water sample to quickly pass through the special column for the large-volume water at a certain flow velocity and enriching residual antibiotics in a water body; (3) eluting the residual antibiotics, enriched in the step (2), in the waterbody by using ammonia methanol, carrying out nitrogen blowing concentration until the residual antibiotics are nearly dry, dissolving the residual antibiotics by using acetonitrile until constant volume and obtaining a test solution; and (4) filtering the test solution obtained in the step (3) through an organic filter membrane, carrying out liquid chromatography-tandem mass spectrometry detectionand collecting and processing measured data of the antibiotics in the water body. According to the method, fast sample loading is carried out by adopting the special column for the large-volume water, the sample loading time of 1L of water sample can be compressed to 11-15min and the whole preprocessing time can be compressed to half an hour, so that a fast and reliable experimental method is provided for detection of five types of 40 antibiotics in the water body.

The invention discloses a method for diagnosing soil contamination by tetracycline antibiotics. The method includes the following steps: placing plant seeds in a group of soil with the antibiotic concentration being in gradient distribution for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds and calculating the average value of the root length; establishing a relation curve between the antibiotics concentration in the soil and the average value of the root length; performing pretreatment on the to-be-detected soil containing antibiotics, placing plant seeds in the to-be-detected soil for cultivation, after three days of cultivation, detecting the length of the root systems of the plant seeds, and calculating the concentration of antibiotics in the to-be-detected soil according to the relation curve, wherein the plant seeds are Zaoshu No.8, the soil is blue purple clay soil, antibiotics are oxytetracycline and chlortetracycline, and the gradient range of antibiotic concentration in the soil is 0-5 mg.kg<-1>. The method provided by the invention can reflect the concentration of antibiotics in the to-be-detected sample, and has the characteristics of being simple, fast and low in cost, and the limitations of the traditional antibiotics detection method are overcome.

The invention discloses a sulfonamide antibiotic detector based on a nanogold-aptamer structure and a detection method. The detector comprises an intelligent device, a multi-flux detection unit, and an optical hardware unit, the multi-flux detection unit comprises a guide tube holder and a plurality of glass guide tubes arranged in a line on the guide tube holder, the glass guide tubes are respectively used for containing reaction solution and to-be-detected solution or reaction solution and standard solutions containing different concentrations of sulfonamide antibiotics, the interior of theoptical hardware unit is provided with an accommodating space for the placement of the multi-flux detection unit, the intelligent device is used for photographing the liquids in the multi-flux detection unit and obtaining the wavelengths of the corresponding solutions according to RGB (Red, Green and Blue) values of pictures, so that the concentration of sulfonamide antibiotic in the to-be-detected solution is obtained, and the optical hardware unit is used for providing uniform illumination for the photographing of the intelligent device. According to the invention, on-site real-time on-linerapid detection on antibiotics can be realized, and the sulfonamide antibiotic detector based on a nanogold-aptamer structure and the detection method can be popularized in various fields such as medical diagnosis, food safety and environmental monitoring.

The invention discloses an antibiotic test paper strip. The antibiotic test paper strip comprises a bottom plate where a sample pad, a combined pad, a reaction membrane and a water absorbing pad sequentially adhere to. A detection line B covered with beta-lactam antigens, a detection line T covered with tetracycline antigens, a detection line S covered with sulfonamide antigens and a quality control line C covered with a goat-anti-mouse IgG are arranged on the reaction membrane, and the combined pad is covered with beta-lactam specific antibody-colloidal gold markers, sulfonamide specific antibody-colloidal gold markers and tetracycline specific antibody-colloidal gold markers. The invention further discloses a preparation method of the test paper strip and application of the test paper strip to milk antibiotic detection. The test paper is fast in detection, convenient to carry, high in sensitivity, easy and convenient to operate, convenient to store, long in shelf life, capable of being industrially produced, low in production cost and capable of detecting beta-lactam antibiotics, tetracycline antibiotics and sulfonamide antibiotics at a time.

The invention discloses aminoglycosides phosphate modifying enzyme for detecting antibiotics. Fluorophore containing or modifying thiol is labeled on the thiol of 147th-170th peptides of the aminoglycosides phosphate modifying enzyme to serve as a fluorescence probe. The invention also discloses a kit and a detection method for detecting antibiotics, wherein the kit is prepared by aminoglycosides phosphate modifying enzyme for detecting antibiotics. Compared with currently common chromatography method, mass spectrography, enzyme-linked immunosorbent assay and other methods, the detection method has the characteristics of high detection speed, convenience in operation, high sensitivity and the like, and thus is an attractive antibiotic detection method.

The invention relates to the field of aquatic product quality monitoring, in particular to the field of monitoring of antibiotics in the aquatic products and more particularly relates to a pretreatment device and method for sulfonamide antibiotic detection of aquatic products and application. By establishment of an online solid-phase extraction and elution pretreatment device for online enrichmentand purification of sulfonamide antibiotics and an online enrichment and purification pretreatment method based on the device, high automation in pretreatment and low loss of the sulfonamide antibiotics in a pretreatment process can be realized. By the method, automatic online purification of complex substrate samples can be realized, high analysis efficiency, low acetonitrile solvent consumption, great purification and decontamination effects, high accuracy and reproducibility of determination results and the like are realized, and the simple, convenience, sensitive and reliable analysis foundation and analysis method are provided for determination of sulfonamides in the aquatic products.