53 results about "Antibiotic testing" patented technology

The invention relates to a method and device for recognizing antibiotics in water by combining Raman spectrum data enhancement with a multi-layer perceptron, and relates to the technical field of antibiotic detection and recognition, and the method comprises the following steps: collecting original Raman spectrums of different types of antibiotics and mixtures thereof by adopting a dendritic nano-silver Raman enhanced substrate; secondly, preprocessing an original Raman spectrum, expanding Raman spectrum data under different matrix conditions through a data enhancement method, generating an expanded spectrum data set, inputting the expanded spectrum data set into the constructed multi-layer perceptron model for model training, and forming sample key features through explanatory variables and feature selection of the SHAP processing model; and finally, inputting spectral data of to-be-identified antibiotics into the model, and identifying the antibiotics in combination with the key features of the sample. It can be seen that the sample data is expanded and enhanced through the data enhancement method, the multi-layer perceptron recognition model with high recognition precision is trained, and the method is used for recognizing antibiotics such as sulfonamide antibiotics in water and improving the recognition accuracy of antibiotic residues.

The invention belongs to the field of antibiotic detection and biosensors, and particularly relates to a colorimetric array sensor for distinguishing different types of antibiotics based on platinum-based nano-enzyme and a detection method of the colorimetric array sensor. The colorimetric array sensor is composed of platinum nano-enzymes with three different structures, namely platinum nano-particles PtNPs, platinum nano-flowers PtNFs and gold-core platinum-shell nano-particles Au (at) PtNPs. According to the antibiotic detection method based on the platinum nano-enzyme colorimetric array sensing system, the peroxidase-like activity of the antibiotics is regulated and controlled through the non-specific interaction of the three platinum nano-enzymes (PtNPs, PtNFs and Au-coated PtNPs) and the antibiotics, a unique'fingerprint 'map is generated in combination with TMB-H2O2 chromogenic reaction, and efficient distinguishing and concentration detection of various antibiotics are achieved.

The invention belongs to the technical field of antibiotic detection, and particularly discloses a synchronous detection method for 42 antibiotics in estuary multi-environment media. The method comprises the following steps: mixing an estuary sample to be detected, a phosphate buffer solution and a methanol-acetonitrile solution of formic acid, carrying out multiple times of ultrasonic extraction, combining extract liquids obtained by the multiple times of ultrasonic extraction, sequentially carrying out pH value regulation and concentration, and then adding water for dilution, so as to obtain an extraction diluent; then carrying out purification treatment on the extraction diluent by adopting a solid-phase extraction method to obtain a to-be-detected solution; adding five antibiotic internal standards into the to-be-detected solution, fixing the volume, detecting the to-be-detected solution by adopting an ultra-high performance liquid chromatography tandem mass spectrometer, and performing data processing on a detection result to obtain the concentration of 42 antibiotics in the to-be-detected estuary sample. The test method disclosed by the invention can be used for trace detection of antibiotics in a complex environmental medium in an estuary region, has the characteristics of low detection limit, good reproducibility and relatively high recovery rate, and can meet analysis requirements.

The invention belongs to the technical field of crystalline materials, and relates to a metal-organic coordination polymer crystal material, in particular to a nickel-based double-ligand coordination polymer crystal material as well as a preparation method and application thereof. The chemical formula of the nickel-based double-ligand coordination polymer crystal material is {[Ni (1, 4-bib) (BDC) (H2O) 2]. (1, 4-bib) 0.5. H2O} n, and the preparation method comprises the following steps: dissolving NiCl2. 6H2O, H2BDC and 1, 4-bib in a DMF / water mixed solution with a volume ratio of 1: 1, sealing, carrying out a solvothermal reaction, heating at a constant temperature of 90.0 DEG C for 3 days, and slowly cooling the system to room temperature to obtain a green bulk crystal G-Ni. The nickel-based double-ligand coordination polymer crystal material can be applied to Ag < + > recognition and antibiotic detection.

The invention discloses a silver nanoparticle / polyvinyl alcohol hydrogel material, a preparation method thereof and application of the silver nanoparticle / polyvinyl alcohol hydrogel material in antibiotic detection. The silver nanoparticle / polyvinyl alcohol hydrogel material comprises monodisperse silver nanoparticles (AgNPs) and polyvinyl alcohol hydrogel, the monodisperse silver nanoparticles are embedded into the polyvinyl alcohol hydrogel, and an AgNPs / PVA composite substrate is formed through calcium ion induced aggregation and freeze thawing crosslinking. The silver nanoparticle / polyvinyl alcohol hydrogel material is used as a hydrogel SERS (Surface Enhanced Raman Scattering) chip for detecting residual veterinary drugs in animal-derived foods, shows good linear response to different antibiotics, has an adding standard recovery rate of 76-86%, is low in cost, and has extremely high sensitivity and relatively strong reproducibility, stability and uniformity.

The invention belongs to the field of antibiotic detection, and relates to detection of norfloxacin (NOR). The invention provides a Luminol-Eu-NCDs ratiometric fluorescent probe as well as a preparation method and application of the Luminol-Eu-NCDs ratiometric fluorescent probe. According to the application, a novel ratiometric fluorescence sensor is constructed through covalent linkage of Luminol-Eu self-assembled and synthesized by AIE and NCDs derived from Listeria monocytogenes. The Luminol-Eu NPs with the AIE effect overcomes the fluorescence quenching characteristic of a traditional fluorophore in a high concentration or aggregation state, so that the reference fluorescence brightness of fluorescence analysis is greatly improved. In addition, due to the introduction of the Luminol-Eu NPs, the sensitivity and the accuracy of NOR detection can also be improved. On the basis, a simple ratio fluorescence sensor with high selectivity is developed, and the ratio fluorescence sensor is used for detecting NOR residues in a milk sample.

The invention provides a water antibiotic pollution detection method and system based on spectral characteristics, and belongs to the technical field of water environment monitoring, analysis and detection.The method comprises the steps that firstly, a surface enhanced Raman spectrogram and oxidation-reduction potential time sequence data of a to-be-detected water sample are synchronously obtained; then, extracting a ratio of the characteristic peak intensity of the target antibiotic from the spectrogram to generate a first characteristic set, and meanwhile, performing wavelet packet decomposition on the potential data and calculating an energy entropy to construct a second characteristic set; then, splicing the two feature sets, and screening out a target feature subset highly related to the concentration through a mutual information maximization algorithm; finally, a support vector projection regression model trained based on a structural risk minimization mechanism is utilized, and accurate detection of the concentration of the target antibiotics in the water sample to be detected is achieved through kernel function mapping and hyperplane projection operation. According to the method, the anti-interference capability and the quantitative precision of antibiotic detection under the complex water body background are improved.

The application discloses a preparation method of an antibiotic selective adsorbent, first, C-BN nanosheet balls are prepared by a simple catalytic thermal evaporation method; second, the C-BN nanosheet balls are used as a main body to limit and load magnetic Fe3O4 nanoparticles; and then mesoporous SiO2 and imprinted polymers are coated on the surface of the C-BN nanosheet balls in sequence, and the tetracycline antibiotic selective adsorbent is obtained after template molecules are eluted. The tetracycline antibiotic selective composite adsorbent with high adsorption capacity is prepared, the high specific surface area of the carrier material and the enrichment antibiotic capacity of the radial structure and the selectivity of the surface molecular imprinting material are fully utilized, the selective adsorption of specific antibiotics in a complex solution is effectively realized, and the adsorption efficiency is effectively improved. The carbon-doped BN and the limited loading magnetic particles enhance the adsorption capacity, and the adsorbent can be quickly recovered and separated under an external magnetic field. Meanwhile, the SiO2 layer and the imprinted polymers enable the antibiotics to be quickly desorbed, and the cycle number is more, so that the antibiotic selective adsorbent has application prospects in the fields of sewage treatment and food antibiotic detection.

The present invention discloses a method for detecting antibiotics based on an upconversion nanoparticle - silk fibroin nanoprobe system. The method includes preparing upconversion nanoparticles synthesized from lanthanide elements, coating water - soluble silica on the upconversion nanoparticles, followed by washing, collecting, and vacuum drying. Fibrous silk fibroin is dissolved, filtered, dialyzed, and concentrated to obtain an aqueous solution of silk fibroin. An antibiotic and a chromogenic agent are mixed to obtain an aqueous solution of a chromogenic agent - antibiotic complex. The upconversion nanoparticles, the aqueous solution of silk fibroin, and the aqueous solution of the chromogenic agent - antibiotic complex are mixed to obtain upconversion nanoparticles coated with silk fibroin and antibiotics. The obtained upconversion nanoparticles are deionized and recovered, and then the fluorescence intensity is measured. The concentration of the antibiotic to be detected is judged according to the fluorescence intensity to achieve the detection of antibiotics. The process of the present invention is simple, easy to operate, low in cost, and high in sensitivity, and has broad application prospects in the fields of quantitative detection of pollutants, antibiotic drugs, etc.

The invention relates to the technical field of chemical application, in particular to application of an SCQDs fluorescent probe in the field of antibiotic detection. When the SCQDs fluorescent probe disclosed by the invention acts with antibiotics, the fluorescence intensity is obviously reduced, the addition of coexisting metal ions does not cause obvious change, F / F0 is still maintained at a relatively low level, and the SCQDs fluorescent probe disclosed by the invention shows excellent anti-interference capability. According to the invention, the SCQDs-TC fluorescent probe is constructed, and the high-selectivity recognition of TC is realized by utilizing the fluorescence quenching effect of TC on SCQDs. According to the invention, the SCQDs-Fe < 3 + >-LEV fluorescent switch probe is constructed, and the specific detection of LEV is realized by using the recovery effect of LEV on Fe < 3 + >-induced fluorescence quenching.

The invention discloses a composite nanomaterial for detecting antibiotics in water, a preparation method and application, and belongs to the technical field of composite nanomaterials, the composite nanomaterial comprises a carbon-based conductive substrate, a metal nanowire network, a two-dimensional carbide lamella and metal oxide nanoparticles; the metal oxide nanoparticles are generated by two-dimensional carbides through in-situ controllable oxidation, all the components form a three-dimensional network with a multi-stage pore structure and multiple active sites, and antibiotic molecules of different structures are recognized through multi-site cooperation of the pi-pi accumulation effect, the coordination bonding effect and the specific chemical adsorption effect; a carbon-based conductive substrate, a metal nanowire network, a two-dimensional carbide lamella and in-situ generated metal oxide nanoparticles are compounded to construct a three-dimensional network system with a multi-stage pore structure and multiple active sites; the carbon-based conductive substrate and the metal nanowire network form an efficient electron transmission channel, so that excellent conductivity is guaranteed.

The application discloses a biosensor and a preparation method and application thereof, and belongs to the technical field of antibiotic detection. The biosensor is obtained by combining a specific capture probe and a functionalized signal probe; the specific capture probe comprises a magnetic nanoparticle base and an antibiotic specific aptamer loaded on the base; and the functionalized signal probe comprises a signal probe and a complementary DNA sequence of the target antibiotic specific aptamer grafted on the signal probe. Specific hybridization between the antibiotic specific aptamer and the complementary DNA forms a pincer-shaped double-stranded structure, which is destroyed when the antibiotic is identified, and the signal probe is released into a solution. The ferrocene and platinum components contained in the probes can catalyze the oxidation of TMB in the presence of H2O2, and the catalytic activity is further enhanced under light irradiation, resulting in a significant colorimetric signal which is easy to observe with naked eyes, so that the purpose of visual detection of antibiotics is achieved.

The invention provides nitrogen-doped sulfonated carbon dots for detecting ceftriaxone sodium as well as a preparation method and application of the nitrogen-doped sulfonated carbon dots, and belongs to the technical field of synthesis of new nano materials and detection of antibiotics. The method comprises the following steps: stirring and dissolving sodium diphenylaminesulfonate and sodium hydroxide in deionized water, heating, then applying direct current to obtain a mixed solution, then carrying out centrifugation, suction filtration and dialysis treatment on the mixed solution, and finally obtaining the carbon dots through a freeze-drying method. The nitrogen-doped sulfonated carbon dots prepared by the method disclosed by the invention show strong blue fluorescence emission, have a fluorescence quenching phenomenon and obvious change of fluorescence color after the ceftriaxone sodium is added, and can be used for measuring a fluorescent nano sensor of the ceftriaxone sodium. The ceftriaxone sodium in an actual sample can be rapidly and sensitively detected on the basis of the nitrogen-doped sulfonated carbon dots, and a new method and a new material are provided for application in the fields of biology, food safety and environmental monitoring.

The utility model discloses a raw material antibiotic detection instrument based on a fluorescent probe technology, which comprises a detection instrument main body, a placing groove arranged at the top of the detection instrument main body and an overturning cover hinged and mounted in the placing groove, and a pore plate is movably mounted in a detection groove arranged at the center of the placing groove; sleeves are fixedly connected below the shell, corresponding to the four corners of the detection groove in the placement groove, of the detector main body, and positioning columns are inserted into the top ends of the sleeves; a casing pipe is also fixedly connected to the position, corresponding to one edge of the detection groove in the placement groove, below the shell of the detector body, an anti-dislocation orientation column is installed at the top end of the casing pipe in an inserted mode, and inserting holes are formed in the positions, corresponding to the positioning column and the anti-dislocation orientation column, of the pore plate. The pore plate disclosed by the utility model can be detached from the placing groove of the detector main body, and the sample bottle is placed outside the detector main body, so that the limitation of the space of the placing groove is avoided, the sample bottle is not easy to turn over due to operation limitation, and the operation is more convenient.

The invention discloses a porous carbon electrode based on gold nanoparticles / poly (3, 4)-ethylenedioxythiophene, a preparation method of the porous carbon electrode and application of the porous carbon electrode in antibiotic detection, and belongs to the technical field of food. The preparation method based on the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode comprises the following steps: (1) engraving the porous carbon electrode by using a laser engraving machine; (2) putting the carbon electrode into a (3, 4)-ethylenedioxythiophene solution, and polymerizing (3, 4)-ethylenedioxythiophene on the porous carbon electrode by utilizing a potentiostatic method; (3) placing the electrode prepared in the step (2) in a chloroauric acid solution, and depositing gold nanoparticles on the electrode through cyclic voltammetry; the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode provided by the invention can sensitively detect chloramphenicol and enrofloxacin at the same time, and can be used for detecting chloramphenicol and enrofloxacin in tap water.

The application discloses an antibiotic detection method based on a two-dimensional metal ene electrochemical sensor and belongs to the technical field of biosensors; the specific steps are as follows: first, ultrasonic dispersion two-dimensional metal ene ink is drop-coated on an electrochemical working electrode to complete preparation of the two-dimensional metal ene electrochemical sensor working electrode; when the electrochemical sensor works, a phosphate buffer solution is used as an electrolyte, an antibiotic to be detected reacts with the two-dimensional metal ene modified on the working electrode to generate an oxidation or reduction reaction, corresponding chemical signals are generated, an electric signal is output through an electrochemical workstation to obtain concentration information of the antibiotic, and super-sensitive, rapid and high-specificity amplification detection of the antibiotic is realized; the application first applies the two-dimensional metal ene to the detection of the antibiotic and fully utilizes the catalytic activity and signal amplification function of the two-dimensional metal ene and the super-sensitivity and rapidness of ECS in biological detection, so that high-specificity, rapid and super-sensitive detection of the antibiotic in a biological sample can be realized.

This invention provides a microfluidic electrochemical biosensor, belonging to the field of antibiotic detection technology. It includes a substrate glass slide and a PDMS chip, with a micron-scale liquid flow channel mechanism on the PDMS chip. The liquid flow channel mechanism includes a flow channel laid in a serpentine pattern on top of the PDMS chip. A delivery tank is located at the port of the flow channel, and the two are connected via a micropump. An electrochemical reaction tank is located at the bend of the flow channel. By setting up a serpentine flow channel on the PDMS chip, and placing an electrochemical reaction tank at each bend of the flow channel, sample liquid can be delivered into each electrochemical reaction tank. Under the delivery of the micropump, the sample liquid can flow into the electrochemical reaction tank at a certain speed, avoiding uneven liquid levels in each electrochemical reaction tank, which could affect the detection of antibiotics in the sample liquid and improve the effectiveness of use.

This invention belongs to the field of antibiotic detection technology. It relates to a photoelectric active material with a P-N covalent bond-bridged heterojunction, its preparation method, and its applications. The RP / g-C3N4 P-N type chemically bonded heterojunction photochemical sensing material prepared by this method benefits from the unique band structure electric field response properties of g-C3N4. Under adjusted bias voltage conditions, this heterojunction material excites a dual-channel photocurrent response at both the anode and cathode, forming a self-detecting signal mechanism. The construction of P-N interface chemical bonds not only provides an efficient directional transport channel for photogenerated electrons but also enhances the structural stability of the system through stress buffering. In summary, the synergistic design of the engineering strategy based on P-N interface bonding bridging and the single-system bipolar signal detection mechanism overcomes the detection limitations of traditional single-channel PEC sensors, significantly improving signal reliability and anti-interference capabilities, and endowing the sensor with wider application adaptability and higher detection confidence.

Antibody-fluorescent nanospheres conjugate for synchronous detection of antibiotics enrofloxacin and ciprofloxacin and preparation method thereof, the steps are as follows: carboxylate-modify polystyrene microspheres on the surface by hydrolysis, and then embed rare earth europium elements inside the microspheres by embedding method to form a combination of polystyrene microspheres and europium elements to obtain fluorescent microspheres; construct a pretreatment system for the fluorescent microspheres, and clean and activate the fluorescent microspheres; couple the fluorescent microspheres with monoclonal antibodies against enrofloxacin or ciprofloxacin; the prepared product is stored in the dark at 2-8°C. The conjugate prepared by the method of the present invention can not only greatly improve the detection sensitivity of the target antibiotics, but also completely shield the cross-interference of the two antibiotics during synchronous detection, solving the problem in the prior art that the detection of enrofloxacin and ciprofloxacin requires separate detection of two indicators, which is both laborious and costly.

The present invention relates to the technical field of antibiotic detection, and particularly relates to a sampling device for detecting antibiotics in sewage, comprising: a detection device, and a sampling mechanism for sampling sewage; the sampling mechanism includes: a sampling tube, a sampling bottle for storing the sampling result, an adjustment assembly for adjusting the position of the sampling bottle, a power source for driving the adjustment assembly to move axially along the sampling tube, and a stirring assembly for stirring the sewage. In the present invention, the power source is started to drive the sampling bottle to move upward, so that the guiding block enters the adjustment groove from the guiding groove. Under the guidance of the adjustment groove, the adjustment plate is driven to rotate by the guiding block, so that the sampling bottle after sampling rotates to the detection position, and the sampling bottle that has not been sampled is transferred to the sampling position, completing the conversion of the sampling bottle.

The present invention belongs to the field of antibiotic detection, and in particular to a method for detecting tetracycline in meat products based on a copper-activated upconversion bismuth molybdate sensing system. The steps are: preparing upconversion nanomaterials and rhodamine derivatives, dissolving the two in methanol to obtain two solutions and mixing them in proportion to construct a sensing system. At the same time, the present invention synthesizes a bismuth molybdate-bismuth sulfide complex by a solvent thermal method, effectively improving the decomposition efficiency of tetracycline and copper ion-tetracycline complex in the solution, and shortening the reaction time. By decomposing the copper ion-tetracycline complex and tetracycline, the remaining tetracycline in the complex and the solution can be avoided from continuing to react with the copper ion, further avoiding the interference of the subsequent reaction on the upconversion fluorescence signal, reducing the detection limit of the method, and improving the detection accuracy. In summary, the present invention provides a novel tetracycline detection method, which does not require expensive instruments, reduces the detection cost, can be used for the detection of actual samples, and has broad application prospects.

The invention successfully constructs a high-sensitivity colorimetric detection method for tobramycin based on flower-like copper sulfide nano enzyme on the basis of peroxidase-like activity of CuS nano enzyme. The flower-like copper sulphide (CuS) nano-enzyme is successfully prepared through a coprecipitation method, the flower-like copper sulphide (CuS) nano-enzyme can catalyze a 3, 3 ', 5, 5'-tetramethyl benzidine-hydrogen peroxide system chromogenic reaction under the acidic condition, and a brand-new colorimetric system for detecting tobramycin is constructed by utilizing the inhibition characteristic of tobramycin on the system. Experimental results show that under the optimal conditions (pH = 4, reaction time 30 min, H220.8 mM, TMB 3.5 mM, and enzyme concentration 15 [mu] g / mL), the detection linear range of the method to the tobramycin is 1 ng / mL-1 mg / mL, the lowest detection concentration is 1 ng / mL, and the sensing system has good specificity to other antibiotics and can effectively distinguish the tobramycin from other antibiotics. The invention not only provides a rapid, sensitive and low-cost new method for detecting tobramycin in water, but also opens up a new thought for antibiotic detection in the fields of environmental monitoring and food safety.

The invention discloses a biosensor as well as a preparation method and application thereof, and belongs to the technical field of antibiotic detection. The biosensor is obtained by combining a specific capture probe and a functionalized signal probe; the specific capture probe comprises a magnetic nanoparticle substrate and an antibiotic specific aptamer loaded on the substrate; the functionalized signal probe comprises a signal probe and a complementary DNA (deoxyribonucleic acid) sequence of a targeted antibiotic specific aptamer grafted on the signal probe. Specific hybridization between the antibiotic specific aptamer and the complementary DNA forms a pincer-like double-chain structure, the structure is destroyed during antibiotic recognition, and the signal probe is released into a solution. The ferrocene and platinum components contained in the probes can catalyze oxidation of TMB in the presence of H2O2, and the catalytic activity of the probes is further enhanced under light irradiation, so that a remarkable colorimetric signal easy to observe by naked eyes is generated, and the aim of visual detection of antibiotics is achieved.

This application provides a method and system for detecting antibiotic pollution in water bodies based on spectral characteristics, belonging to the field of water environment monitoring and analysis technology. First, this application simultaneously acquires the surface-enhanced Raman spectrum and redox potential time-series data of the water sample to be tested. Next, the ratio of the characteristic peak intensities of the target antibiotic is extracted from the spectrum to generate a first feature set. Simultaneously, wavelet packet decomposition is performed on the potential data, and energy entropy is calculated to construct a second feature set. Subsequently, the two feature sets are concatenated, and a mutual information maximization algorithm is used to filter out the target feature subset highly correlated with concentration. Finally, using a support vector projection regression model trained based on a structural risk minimization mechanism, and through kernel function mapping and hyperplane projection operations, the accurate detection of the target antibiotic concentration in the water sample is achieved. This application improves the anti-interference capability and quantitative accuracy of antibiotic detection in complex water body backgrounds.

The application discloses a kind of ciprofloxacin orange carbon dots fluorescence biomimetic sensing materials and preparation method and application, belong to polymer optical sensing material preparation technical field and antibiotic detection method technical field;The method comprises the following steps: (1) ciprofloxacin solution and methacrylic acid are added to anhydrous ethanol, stirred uniformly, then ethylene glycol dimethyl acrylate and azobisisobutyronitrile are added, nitrogen is removed oxygen, seal, sequentially carry out thermal polymerization, cooling, ultrasonic centrifugation and drying, obtain molecularly imprinted polymer MIPs;(2) by imprinting post-modification strategy, orange carbon dots are mixed with the molecularly imprinted polymer MIPs uniformly, then sequentially carry out ultrasonic centrifugation, drying, obtain ciprofloxacin orange carbon dots fluorescence biomimetic sensing material.The fluorescent material prepared by the method of the application has long-wavelength emission orange carbon dots as fluorescent signal, combined with the imprinting post-modification strategy of molecular imprinting technology, the sensitive detection of ciprofloxacin can be realized;And the preparation method is simple in operation, low in cost, and has good application prospect for the detection of ciprofloxacin in food.

The invention belongs to the technical field of detection of antibiotics in soil, and particularly relates to a soil antibiotic distribution sampling detection method which comprises the following steps: S1, selecting a to-be-detected area, cleaning ground surface soil and sundries, and selecting points and punching detection holes in the to-be-detected area; s2, spraying water to the hole wall of the detection hole, and collecting a dissolving solution flowing downwards along the hole wall at the to-be-detected depth in the detection hole; and S3, taking the collected dissolving solution back to a laboratory, and carrying out detection and analysis. According to the device, antibiotics in soil in the range are dissolved by water flow to form a dissolving solution, the water absorption device only collects the dissolving solution of the soil in the detection depth range between the water injection spray head and the last-stage flow guide conical cylinder, and the distribution condition of the antibiotics in the soil in the specific depth is accurately collected and detected.

This invention relates to the field of antibiotic detection technology, specifically to a high-performance liquid chromatography-electrostatic orbital trap (HPLC-EORT) method for quantitative detection of multiple antibiotics in groundwater. The method includes the following steps: solid-phase extraction of the groundwater sample to obtain a test solution; adding an antibiotic internal standard to the test solution and making up to a fixed volume; and then using HPLC-EORT high-resolution mass spectrometry to detect the concentration of multiple antibiotics in the groundwater. This invention establishes a standardized pretreatment procedure to ensure effective extraction of various compounds and minimize losses. Simultaneously, it optimizes the gradient elution procedure of liquid chromatography and specific settings of mass spectrometry parameters to achieve optimal separation and detection of 83 antibiotics in groundwater. This invention can reduce the detection limit of the testing method to the ppt level; the atomic weight of the quantified ions is accurate to four decimal places, improving the resolution and accuracy of screening multiple antibiotics in groundwater.

The application discloses a MOF / MIP / QCM sensor for in-situ detection of quinolone antibiotics, a preparation method and application, and belongs to the technical field of environmental antibiotic detection. The preparation method is as follows: 1) quinolone antibiotic template molecules and functional monomers are added to an organic solvent, and after being dissolved, MOF materials, a crosslinking agent and an initiator are sequentially added to obtain a prepolymerization solution; 2) the prepolymerization solution is subjected to a polymerization reaction under the protection of an inert gas, and after the template molecules are removed by washing, the MOF-based molecularly imprinted polymer is dried to obtain the MOF / MIP / QCM sensor. The sensor is connected with a quartz crystal microbalance detection device, and then placed in a water body to be detected; the specific adsorption of the sensor to quinolone antibiotics causes the characteristic of a decrease in the resonance frequency of the quartz crystal, and the in-situ rapid quantitative detection of quinolone antibiotics is realized by measuring the frequency change.

This invention belongs to the field of fluorescent carbon nanomaterial preparation and antibiotic detection technology, disclosing fluorescent carbon dots and their hydrogels and applications, as well as a method for detecting enrofloxacin. A fluorescent carbon dot hydrogel was constructed based on fluorescent carbon dots, and an enrofloxacin detection method was constructed based on fluorescent carbon dots or the fluorescent carbon dot hydrogel. The detection method involves mixing fluorescent carbon dots with the sample to be tested, according to... 411 / I 508 Enrofloxacin quantification is achieved by fitting the value of the G / B ratio to the enrofloxacin concentration; alternatively, the fluorescent carbon dot hydrogel is immersed in the sample and incubated for a sufficient time, and a colorimetric image is obtained under 365 nm ultraviolet light. The G / B value of the fluorescent carbon dot hydrogel region in the colorimetric image is obtained, and the quantitative analysis of enrofloxacin is achieved based on the fitting relationship between the G / B value and the enrofloxacin concentration. This invention can improve the accuracy of enrofloxacin detection and, furthermore, enable rapid and high-throughput detection of enrofloxacin.