29 results about "Antibiotic testing" patented technology

The invention relates to a method and device for recognizing antibiotics in water by combining Raman spectrum data enhancement with a multi-layer perceptron, and relates to the technical field of antibiotic detection and recognition, and the method comprises the following steps: collecting original Raman spectrums of different types of antibiotics and mixtures thereof by adopting a dendritic nano-silver Raman enhanced substrate; secondly, preprocessing an original Raman spectrum, expanding Raman spectrum data under different matrix conditions through a data enhancement method, generating an expanded spectrum data set, inputting the expanded spectrum data set into the constructed multi-layer perceptron model for model training, and forming sample key features through explanatory variables and feature selection of the SHAP processing model; and finally, inputting spectral data of to-be-identified antibiotics into the model, and identifying the antibiotics in combination with the key features of the sample. It can be seen that the sample data is expanded and enhanced through the data enhancement method, the multi-layer perceptron recognition model with high recognition precision is trained, and the method is used for recognizing antibiotics such as sulfonamide antibiotics in water and improving the recognition accuracy of antibiotic residues.

The invention provides a water antibiotic pollution detection method and system based on spectral characteristics, and belongs to the technical field of water environment monitoring, analysis and detection.The method comprises the steps that firstly, a surface enhanced Raman spectrogram and oxidation-reduction potential time sequence data of a to-be-detected water sample are synchronously obtained; then, extracting a ratio of the characteristic peak intensity of the target antibiotic from the spectrogram to generate a first characteristic set, and meanwhile, performing wavelet packet decomposition on the potential data and calculating an energy entropy to construct a second characteristic set; then, splicing the two feature sets, and screening out a target feature subset highly related to the concentration through a mutual information maximization algorithm; finally, a support vector projection regression model trained based on a structural risk minimization mechanism is utilized, and accurate detection of the concentration of the target antibiotics in the water sample to be detected is achieved through kernel function mapping and hyperplane projection operation. According to the method, the anti-interference capability and the quantitative precision of antibiotic detection under the complex water body background are improved.

The application discloses a preparation method of an antibiotic selective adsorbent, first, C-BN nanosheet balls are prepared by a simple catalytic thermal evaporation method; second, the C-BN nanosheet balls are used as a main body to limit and load magnetic Fe3O4 nanoparticles; and then mesoporous SiO2 and imprinted polymers are coated on the surface of the C-BN nanosheet balls in sequence, and the tetracycline antibiotic selective adsorbent is obtained after template molecules are eluted. The tetracycline antibiotic selective composite adsorbent with high adsorption capacity is prepared, the high specific surface area of the carrier material and the enrichment antibiotic capacity of the radial structure and the selectivity of the surface molecular imprinting material are fully utilized, the selective adsorption of specific antibiotics in a complex solution is effectively realized, and the adsorption efficiency is effectively improved. The carbon-doped BN and the limited loading magnetic particles enhance the adsorption capacity, and the adsorbent can be quickly recovered and separated under an external magnetic field. Meanwhile, the SiO2 layer and the imprinted polymers enable the antibiotics to be quickly desorbed, and the cycle number is more, so that the antibiotic selective adsorbent has application prospects in the fields of sewage treatment and food antibiotic detection.

The invention discloses a composite nanomaterial for detecting antibiotics in water, a preparation method and application, and belongs to the technical field of composite nanomaterials, the composite nanomaterial comprises a carbon-based conductive substrate, a metal nanowire network, a two-dimensional carbide lamella and metal oxide nanoparticles; the metal oxide nanoparticles are generated by two-dimensional carbides through in-situ controllable oxidation, all the components form a three-dimensional network with a multi-stage pore structure and multiple active sites, and antibiotic molecules of different structures are recognized through multi-site cooperation of the pi-pi accumulation effect, the coordination bonding effect and the specific chemical adsorption effect; a carbon-based conductive substrate, a metal nanowire network, a two-dimensional carbide lamella and in-situ generated metal oxide nanoparticles are compounded to construct a three-dimensional network system with a multi-stage pore structure and multiple active sites; the carbon-based conductive substrate and the metal nanowire network form an efficient electron transmission channel, so that excellent conductivity is guaranteed.

The application discloses a biosensor and a preparation method and application thereof, and belongs to the technical field of antibiotic detection. The biosensor is obtained by combining a specific capture probe and a functionalized signal probe; the specific capture probe comprises a magnetic nanoparticle base and an antibiotic specific aptamer loaded on the base; and the functionalized signal probe comprises a signal probe and a complementary DNA sequence of the target antibiotic specific aptamer grafted on the signal probe. Specific hybridization between the antibiotic specific aptamer and the complementary DNA forms a pincer-shaped double-stranded structure, which is destroyed when the antibiotic is identified, and the signal probe is released into a solution. The ferrocene and platinum components contained in the probes can catalyze the oxidation of TMB in the presence of H2O2, and the catalytic activity is further enhanced under light irradiation, resulting in a significant colorimetric signal which is easy to observe with naked eyes, so that the purpose of visual detection of antibiotics is achieved.

The application discloses an antibiotic detection method based on a two-dimensional metal ene electrochemical sensor and belongs to the technical field of biosensors; the specific steps are as follows: first, ultrasonic dispersion two-dimensional metal ene ink is drop-coated on an electrochemical working electrode to complete preparation of the two-dimensional metal ene electrochemical sensor working electrode; when the electrochemical sensor works, a phosphate buffer solution is used as an electrolyte, an antibiotic to be detected reacts with the two-dimensional metal ene modified on the working electrode to generate an oxidation or reduction reaction, corresponding chemical signals are generated, an electric signal is output through an electrochemical workstation to obtain concentration information of the antibiotic, and super-sensitive, rapid and high-specificity amplification detection of the antibiotic is realized; the application first applies the two-dimensional metal ene to the detection of the antibiotic and fully utilizes the catalytic activity and signal amplification function of the two-dimensional metal ene and the super-sensitivity and rapidness of ECS in biological detection, so that high-specificity, rapid and super-sensitive detection of the antibiotic in a biological sample can be realized.

This invention provides a microfluidic electrochemical biosensor, belonging to the field of antibiotic detection technology. It includes a substrate glass slide and a PDMS chip, with a micron-scale liquid flow channel mechanism on the PDMS chip. The liquid flow channel mechanism includes a flow channel laid in a serpentine pattern on top of the PDMS chip. A delivery tank is located at the port of the flow channel, and the two are connected via a micropump. An electrochemical reaction tank is located at the bend of the flow channel. By setting up a serpentine flow channel on the PDMS chip, and placing an electrochemical reaction tank at each bend of the flow channel, sample liquid can be delivered into each electrochemical reaction tank. Under the delivery of the micropump, the sample liquid can flow into the electrochemical reaction tank at a certain speed, avoiding uneven liquid levels in each electrochemical reaction tank, which could affect the detection of antibiotics in the sample liquid and improve the effectiveness of use.

This invention belongs to the field of antibiotic detection technology. It relates to a photoelectric active material with a P-N covalent bond-bridged heterojunction, its preparation method, and its applications. The RP / g-C3N4 P-N type chemically bonded heterojunction photochemical sensing material prepared by this method benefits from the unique band structure electric field response properties of g-C3N4. Under adjusted bias voltage conditions, this heterojunction material excites a dual-channel photocurrent response at both the anode and cathode, forming a self-detecting signal mechanism. The construction of P-N interface chemical bonds not only provides an efficient directional transport channel for photogenerated electrons but also enhances the structural stability of the system through stress buffering. In summary, the synergistic design of the engineering strategy based on P-N interface bonding bridging and the single-system bipolar signal detection mechanism overcomes the detection limitations of traditional single-channel PEC sensors, significantly improving signal reliability and anti-interference capabilities, and endowing the sensor with wider application adaptability and higher detection confidence.

The invention successfully constructs a high-sensitivity colorimetric detection method for tobramycin based on flower-like copper sulfide nano enzyme on the basis of peroxidase-like activity of CuS nano enzyme. The flower-like copper sulphide (CuS) nano-enzyme is successfully prepared through a coprecipitation method, the flower-like copper sulphide (CuS) nano-enzyme can catalyze a 3, 3 ', 5, 5'-tetramethyl benzidine-hydrogen peroxide system chromogenic reaction under the acidic condition, and a brand-new colorimetric system for detecting tobramycin is constructed by utilizing the inhibition characteristic of tobramycin on the system. Experimental results show that under the optimal conditions (pH = 4, reaction time 30 min, H220.8 mM, TMB 3.5 mM, and enzyme concentration 15 [mu] g / mL), the detection linear range of the method to the tobramycin is 1 ng / mL-1 mg / mL, the lowest detection concentration is 1 ng / mL, and the sensing system has good specificity to other antibiotics and can effectively distinguish the tobramycin from other antibiotics. The invention not only provides a rapid, sensitive and low-cost new method for detecting tobramycin in water, but also opens up a new thought for antibiotic detection in the fields of environmental monitoring and food safety.

The invention discloses a biosensor as well as a preparation method and application thereof, and belongs to the technical field of antibiotic detection. The biosensor is obtained by combining a specific capture probe and a functionalized signal probe; the specific capture probe comprises a magnetic nanoparticle substrate and an antibiotic specific aptamer loaded on the substrate; the functionalized signal probe comprises a signal probe and a complementary DNA (deoxyribonucleic acid) sequence of a targeted antibiotic specific aptamer grafted on the signal probe. Specific hybridization between the antibiotic specific aptamer and the complementary DNA forms a pincer-like double-chain structure, the structure is destroyed during antibiotic recognition, and the signal probe is released into a solution. The ferrocene and platinum components contained in the probes can catalyze oxidation of TMB in the presence of H2O2, and the catalytic activity of the probes is further enhanced under light irradiation, so that a remarkable colorimetric signal easy to observe by naked eyes is generated, and the aim of visual detection of antibiotics is achieved.

This application provides a method and system for detecting antibiotic pollution in water bodies based on spectral characteristics, belonging to the field of water environment monitoring and analysis technology. First, this application simultaneously acquires the surface-enhanced Raman spectrum and redox potential time-series data of the water sample to be tested. Next, the ratio of the characteristic peak intensities of the target antibiotic is extracted from the spectrum to generate a first feature set. Simultaneously, wavelet packet decomposition is performed on the potential data, and energy entropy is calculated to construct a second feature set. Subsequently, the two feature sets are concatenated, and a mutual information maximization algorithm is used to filter out the target feature subset highly correlated with concentration. Finally, using a support vector projection regression model trained based on a structural risk minimization mechanism, and through kernel function mapping and hyperplane projection operations, the accurate detection of the target antibiotic concentration in the water sample is achieved. This application improves the anti-interference capability and quantitative accuracy of antibiotic detection in complex water body backgrounds.

The application discloses a kind of ciprofloxacin orange carbon dots fluorescence biomimetic sensing materials and preparation method and application, belong to polymer optical sensing material preparation technical field and antibiotic detection method technical field;The method comprises the following steps: (1) ciprofloxacin solution and methacrylic acid are added to anhydrous ethanol, stirred uniformly, then ethylene glycol dimethyl acrylate and azobisisobutyronitrile are added, nitrogen is removed oxygen, seal, sequentially carry out thermal polymerization, cooling, ultrasonic centrifugation and drying, obtain molecularly imprinted polymer MIPs;(2) by imprinting post-modification strategy, orange carbon dots are mixed with the molecularly imprinted polymer MIPs uniformly, then sequentially carry out ultrasonic centrifugation, drying, obtain ciprofloxacin orange carbon dots fluorescence biomimetic sensing material.The fluorescent material prepared by the method of the application has long-wavelength emission orange carbon dots as fluorescent signal, combined with the imprinting post-modification strategy of molecular imprinting technology, the sensitive detection of ciprofloxacin can be realized;And the preparation method is simple in operation, low in cost, and has good application prospect for the detection of ciprofloxacin in food.

The invention belongs to the technical field of detection of antibiotics in soil, and particularly relates to a soil antibiotic distribution sampling detection method which comprises the following steps: S1, selecting a to-be-detected area, cleaning ground surface soil and sundries, and selecting points and punching detection holes in the to-be-detected area; s2, spraying water to the hole wall of the detection hole, and collecting a dissolving solution flowing downwards along the hole wall at the to-be-detected depth in the detection hole; and S3, taking the collected dissolving solution back to a laboratory, and carrying out detection and analysis. According to the device, antibiotics in soil in the range are dissolved by water flow to form a dissolving solution, the water absorption device only collects the dissolving solution of the soil in the detection depth range between the water injection spray head and the last-stage flow guide conical cylinder, and the distribution condition of the antibiotics in the soil in the specific depth is accurately collected and detected.

This invention relates to the field of antibiotic detection technology, specifically to a high-performance liquid chromatography-electrostatic orbital trap (HPLC-EORT) method for quantitative detection of multiple antibiotics in groundwater. The method includes the following steps: solid-phase extraction of the groundwater sample to obtain a test solution; adding an antibiotic internal standard to the test solution and making up to a fixed volume; and then using HPLC-EORT high-resolution mass spectrometry to detect the concentration of multiple antibiotics in the groundwater. This invention establishes a standardized pretreatment procedure to ensure effective extraction of various compounds and minimize losses. Simultaneously, it optimizes the gradient elution procedure of liquid chromatography and specific settings of mass spectrometry parameters to achieve optimal separation and detection of 83 antibiotics in groundwater. This invention can reduce the detection limit of the testing method to the ppt level; the atomic weight of the quantified ions is accurate to four decimal places, improving the resolution and accuracy of screening multiple antibiotics in groundwater.

The application discloses a MOF / MIP / QCM sensor for in-situ detection of quinolone antibiotics, a preparation method and application, and belongs to the technical field of environmental antibiotic detection. The preparation method is as follows: 1) quinolone antibiotic template molecules and functional monomers are added to an organic solvent, and after being dissolved, MOF materials, a crosslinking agent and an initiator are sequentially added to obtain a prepolymerization solution; 2) the prepolymerization solution is subjected to a polymerization reaction under the protection of an inert gas, and after the template molecules are removed by washing, the MOF-based molecularly imprinted polymer is dried to obtain the MOF / MIP / QCM sensor. The sensor is connected with a quartz crystal microbalance detection device, and then placed in a water body to be detected; the specific adsorption of the sensor to quinolone antibiotics causes the characteristic of a decrease in the resonance frequency of the quartz crystal, and the in-situ rapid quantitative detection of quinolone antibiotics is realized by measuring the frequency change.

This invention belongs to the field of fluorescent carbon nanomaterial preparation and antibiotic detection technology, disclosing fluorescent carbon dots and their hydrogels and applications, as well as a method for detecting enrofloxacin. A fluorescent carbon dot hydrogel was constructed based on fluorescent carbon dots, and an enrofloxacin detection method was constructed based on fluorescent carbon dots or the fluorescent carbon dot hydrogel. The detection method involves mixing fluorescent carbon dots with the sample to be tested, according to... 411 / I 508 Enrofloxacin quantification is achieved by fitting the value of the G / B ratio to the enrofloxacin concentration; alternatively, the fluorescent carbon dot hydrogel is immersed in the sample and incubated for a sufficient time, and a colorimetric image is obtained under 365 nm ultraviolet light. The G / B value of the fluorescent carbon dot hydrogel region in the colorimetric image is obtained, and the quantitative analysis of enrofloxacin is achieved based on the fitting relationship between the G / B value and the enrofloxacin concentration. This invention can improve the accuracy of enrofloxacin detection and, furthermore, enable rapid and high-throughput detection of enrofloxacin.

The application relates to the technical field of veterinary drug detection, in particular to a veterinary drug antibiotic residue detection device, which comprises a base, the top front part of the base is provided with an antibiotic detector, the top of a lifting mechanism is provided with a processing bin, the inside of the lifting mechanism is provided with a differential pressure mechanism, and the inside of the processing bin is provided with a blowing mechanism; the driving mechanism comprises a support, the rear side of the support is slidably connected to the rear end of an L-shaped frame, a servo motor output end penetrates through the support and is fixedly connected with a rotating rod, the bottom end of the outer side of the rotating rod is fixedly connected with a cutter, the upper part of the outer side of the rotating rod is fixedly connected with an impeller, and the rear end of a gas inlet bin is fixedly connected with a gas pipe one; the driving mechanism and the lifting mechanism are arranged, the uniformity of the chopping effect of each layer of the sample can be effectively guaranteed when the sample for testing is prepared, large pieces of the sample in each layer that are not in contact with the cutter due to weak fluidity are prevented, and the chemical analysis efficiency is avoided from being affected.

The invention relates to the technical field of fluorescence sensing and the field of antibiotic fluorescence detection, in particular to a cadmium (II) MOFs (Metal-Organic Frameworks) material as well as a preparation method and application thereof in antibiotic detection. A cadmium (II) MOFs material [Cd (cpt) 2H2O] n can be prepared by adopting a one-pot hydrothermal reaction, the molecular formula is C56H38CdN6O7, and Hcpt is 4 '-(4-(4-carboxyl phenoxy) phenyl)-4, 2': 6 ', 4' '-terpyridyl. The preparation method has the advantages of mild conditions, simplicity and convenience in operation, high yield, good repeatability and the like. The cadmium (II) MOFs material in the prepared cadmium element coordination polymer has good structural stability and fluorescence characteristics and shows high selectivity and sensitivity to tetracycline antibiotics such as tetracycline hydrochloride, aureomycin and doxycycline (THC, CTC and DOX) in antibiotic detection, and the concentration of the tetracycline antibiotics can be preferentially detected through a fluorescence quenching phenomenon.

The invention discloses an in-situ growth sandwich silver carbon structure portable probe as well as a preparation method and a detection method thereof, and belongs to the technical field of antibiotic detection. According to the method, flexible carbon cloth serves as a supporting substrate, silver nanoparticles grow on the surface of the flexible carbon cloth in situ by means of a chemical plating method, after modification is conducted through a silane coupling agent, a specific recognition polymer layer with fluoroquinolone antibiotics as template molecules is synthesized on the surface of the flexible carbon cloth through a molecular imprinting technology (MIP), and the specific recognition polymer layer is prepared. And a'carbon cloth-silver nanoparticle-molecularly imprinted polymer 'sandwich structure is formed. According to the probe, high selectivity of MIP and high sensitivity of surface enhanced Raman spectroscopy (SERS) are combined, and the problems of small specific surface area, low mass transfer efficiency, high cost and the like of a traditional rigid substrate are effectively solved by utilizing the large specific surface area and flexibility of a carbon cloth substrate. The probe has the advantages of high sensitivity, good selectivity, strong stability, simplicity and convenience in operation, low cost and the like, and is suitable for in-situ rapid detection of trace antibiotics in environmental water samples, food and biological samples.

The invention discloses a method for rapidly identifying and quantifying antibiotics in milk based on a gold nano gap monolayer and artificial intelligence, and belongs to the technical field of food safety biology. Raman detection is performed on antibiotics in milk by adopting a gold nano-gap single-layer substrate, meanwhile, a deep learning technology is combined, and spectral characteristics of different antibiotics are accurately distinguished for qualitative and quantitative analysis by automatically extracting spatial local characteristics in a Raman spectrum; the antibiotic detection method is free of pretreatment, high in detection speed and accurate in result.

This invention provides nitrogen-doped sulfonated carbon dots for detecting ceftriaxone sodium, their preparation method, and their applications, belonging to the fields of nanomaterial synthesis and antibiotic detection technology. The method involves: dissolving sodium diphenylamine sulfonate and sodium hydroxide in deionized water under stirring and heating; applying a direct current to obtain a mixed solution; centrifuging, filtration, and dialysis of the mixed solution; and finally, freeze-drying to obtain carbon dots. The nitrogen-doped sulfonated carbon dots prepared by this method exhibit strong blue fluorescence emission, and show fluorescence quenching and a significant change in fluorescence color upon the addition of ceftriaxone sodium, making them suitable as fluorescent nanosensors for detecting ceftriaxone sodium. Furthermore, this invention, based on the ability of nitrogen-doped sulfonated carbon dots to rapidly and sensitively detect ceftriaxone sodium in real samples, provides a new method and material for applications in the fields of biology, food safety, and environmental monitoring.

The present invention belongs to the technical field of antibiotic detection. Disclosed are a preparation method for a flexible paper-based sensor used for detecting tetracycline residues in meat products, and a method for detecting tetracycline residues in meat products by using the flexible paper-based sensor. The flexible paper-based sensor is obtained by mixing a UCNPs-cDNA signal probe and an Apt-MIL-53(Fe)@Cu2+ capture probe to obtain a detection probe; and then adding, in a dropwise manner, the detection probe to the central area of a paper-based carrier. By means of the specific recognition effect of Apt, which is located on the surface of the capture probe, on tetracycline, the constructed flexible paper-based sensor has relatively good specificity and anti-interference performance, and can accurately recognize tetracycline, thereby improving the detection accuracy; and the obtained flexible paper-based sensor is convenient to carry, and can achieve rapid and sensitive on-site detection of tetracycline in meat products.

The invention discloses a voltage-adjustable precious metal / semiconductor layer-by-layer assembled SERS (Surface Enhanced Raman Scattering) sensing substrate as well as a preparation method and antibiotic detection application thereof, and belongs to the technical field of nanomaterial science and laser Raman detection. The preparation method comprises the following steps: firstly, preparing an Au NPs (at) Ag thin film, then transferring a tungsten oxide thin film consisting of tungsten oxide nanowires to a gold mirror of conductive glass, then carrying out oxygen plasma treatment on the tungsten oxide thin film, and then transferring the Au NPs (at) Ag thin film to the tungsten oxide thin film subjected to the oxygen plasma treatment, so as to obtain the voltage-adjustable noble metal / semiconductor layer-by-layer assembled SERS sensing substrate. Based on a layer-by-layer self-assembly technology, precious metal with unique optical and electrical properties and tungsten oxide nanowires WO3-x NWs are combined, the physical and chemical synergistic enhancement effect of the precious metal and the tungsten oxide nanowires WO3-x NWs is brought into full play, the energy band structure of the SERS sensing substrate under stimulation of different voltages is adjustable, and the SERS sensing substrate has a good application prospect in the field of detection.

The utility model discloses a device for separating, enriching and extracting antibiotics in water, and relates to the technical field of antibiotic extraction.The device comprises a PH value adjusting device, a filtering device, an extracting device, an evaporating device and a cooling device.According to the device, the PH value adjusting device and the filtering device are arranged, so that the stability of the PH value in the water before the antibiotics are extracted is guaranteed; impurities possibly affecting the extraction efficiency in the water body are filtered out, the extraction effect is further guaranteed, the extraction efficiency is improved, the extracted antibiotics are purified and enriched through the evaporation device, the next step of antibiotic detection is facilitated, the cooling device is arranged, the solvent is recycled, direct emission of the gasified solvent is avoided, the environment is protected, and resources are saved. Finally, the PH value adjusting device, the filtering device, the extraction device, the evaporation device and the cooling device are connected through a first pipeline, a second pipeline, a third pipeline and a fourth pipeline, the operation process is simplified, and the extraction operation time is saved.

The utility model provides a kind of biological sensor antibiotic analysis equipment, belong to antibiotic analysis technical field.The rear of the antibiotic detection main part is provided with connecting line, and electrode clip assembly is set in one end of connecting line.Through the electrode clip assembly of setting in one end of connecting line on antibiotic detection main part, electrode clip assembly adopts rectangular plate, metal clamping plate and locking piece to be made of, can be clamped when clamping, electrode stick is clamped by metal clamping plate, and electrode stick is tightly clamped by two metal clamping plates using locking piece, avoid appearing in subsequent use process Shaking, even appear the situation of falling off, guarantee the stable detection of carrying out.

The invention provides a nucleic acid molecule labeled with a fluorescent dye and a method for detecting oxytetracycline by using the nucleic acid molecule, and belongs to the technical field of antibiotic detection. Nucleic acid molecules (SEQ ID NO: 1) capable of specifically recognizing oxytetracycline are adopted as nucleic acid aptamers, and after nucleic acid molecules marked with fluorescent dye are selectively combined with oxytetracycline, the fluorescence intensity and fluorescence anisotropy signals of the nucleic acid molecules are obviously changed, so that a method for measuring the content of oxytetracycline in a sample to be measured is developed. The detection limit of the detection method for oxytetracycline reaches nM level, and the sensitivity is high.

This invention discloses a method and application for colorimetric analysis of antibiotics using a paper-based detection chip. The method includes: preparing a paper-based detection unit; immersing the paper-based detection unit in a NiCo@C HNs solution modified with nucleic acid aptamers; drying to obtain a paper-based detection chip; adding samples containing different concentrations of antibiotics for reaction; then adding a solution containing TMB and H2O2 for colorimetric reaction; using a mobile phone to identify color intensity to obtain RGB or grayscale values; selecting the linear curve with the highest fitting degree between antibiotic concentration and RGB or grayscale values ​​as the antibiotic detection standard curve; and then quantitatively detecting the antibiotic content based on the RGB or grayscale values ​​of the measured sample. Antibiotics can also be qualitatively detected based on colorimetric analysis. This method has advantages such as low cost, simple operation, fast response, strong anti-interference ability, and on-site detection capability, and can be applied to the detection of antibiotic residues in the environment and food.

The invention belongs to the technical field of electrochemical luminescence, and particularly relates to a target response type ECL aptamer sensing platform and a preparation method and application thereof. The covalent organic framework material based on imine bond connection is Btt-Tpa-COF (Btt-Tapt-COF) and Btt-Tapt-COF (Btt-Tapt-COF). According to the present invention, the Btt-Tap-COF is adopted as the efficient luminous body, and the three-dimensional DNA hydrogel is adopted as the intelligent signal switch, such that the high-sensitivity detection of the lincomycin is provided; when the target lincomycin is deleted, the DNA hydrogel can quench an ECL signal of a Btt-Tapt-COF / TEA system, and a signal closing state is formed. When the lincomycin exists, the lincomycin can be specifically combined with the aptamer sequence in the DNA hydrogel, so that the network structure of the DNA hydrogel is dissociated, and a signal opening state is formed. Therefore, an antibiotic detection method based on ECL is constructed, and the method has potential application prospects.

The utility model discloses a detachable kit for detecting various antibiotics, which belongs to the technical field of kits and comprises a fixed disc, a filling component and a filling mold are arranged at the top of the fixed disc, an opening and closing component is arranged in the filling mold, a plurality of connecting grooves are arranged on the surface of the fixed disc, and detection cups are arranged at the bottoms of the connecting grooves. And the detection cup is filled with a culture medium. Through the cooperative use of the filling mold and the fixed disc, the consistency of the filling quantity among different detection cups is ensured during filling, the pipeline pollution is prevented through physical isolation during storage, and the alignment of the through hole and the liquid outlet pipeline is controlled by arranging the opening and closing assembly and driving the rotating disc to rotate through the driving lever; mechanical fluid on-off control is achieved, meanwhile, the shifting rod can visually display the opening and closing state, and the adjusting groove can provide clear operation limiting.