328results about How to "Resolve Sensitivity" patented technology

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

The invention relates to a thin-film ultrasonic transducer and a preparation method thereof. The thin-film ultrasonic transducer comprises a capacitance transducer unit, wherein the capacitance transducer unit comprises a capacitance second electrode (126), an insulating layer (125), an air micro-gap (130), a supporting component (128) and a piezoelectric first electrode (123) from bottom to top. The thin-film ultrasonic transducer is characterized in that the thin-film ultrasonic transducer also comprises a piezoelectric layer (122) positioned on the piezoelectric first electrode (123) and a piezoelectric second electrode (121) positioned on the piezoelectric layer (122) from bottom to top in turn; the supporting component (128) is a bendable piezoelectric vibrating diaphragm; and an inward-concave position in the lower center of the supporting component and the insulating layer (125) form a closed air micro-gap cavity. The invention also provides three methods for preparing the thin-film ultrasonic transducer, prepares all the components from bottom to top in turn, and particularly provides a plurality of modes for preparing the air micro-gap.

The invention provides a time-resolved single-photon counting two-dimensional imaging system and a time-resolved single-photon counting two-dimensional imaging method and belongs to the technical field of extremely-weak light detection. A trigger 2 is triggered to start sampling, centralized sampling is performed at t time intervals, and measurement and counting are performed if light comes at the intervals, so that time resolving of an extremely-weak light object is realized, and a time sequence image is generated. Imaging is performed on the basis of a compressive sensing (CS) theory, a digital micro-mirror device (DMD5) performs linear random projection on a compressible two-dimensional image, the compressible two-dimensional image is optically modulated and then synchronously detected by using a single-photon counter, and a high-resolution extremely-weak light image can be reconstructed by a small amount of sampling operation. The measurement process is linear and non-adaptive, the reconstruction process is non-linear, and the invention has the advantages of high generality, robustness, expandability, superposition and computation asymmetry, and can be widely applied to the fields of life science, medical imaging, data acquisition, communication, astronomy, military affairs, hyper-spectral imaging and quantum measurement.

The invention provides a micro-electromechanical system (MEMS) triaxial accelerometer and a manufacturing method thereof. The MEMS triaxial accelerometer comprises a sensitive device layer, an upper cover board layer and a lower supporting body layer, wherein clearances are reserved between the sensitive device layer and the upper cover board layer as well as between the sensitive device layer and the lower supporting body layer; the sensitive device layer comprises a supporting frame body, an elastic beam, three independent sensitive mass blocks, movable comb teeth, fixed comb teeth and an electrode; the three independent sensitive mass blocks in the sensitive device layer are used for detecting acceleration signals of three axes X, Y and Z respectively; an acceleration sensor in each direction is hung in the supporting frame body through the corresponding sensitive mass block by the elastic beam which is only sensitive to the detection direction; a plurality of pairs of movable comb teeth are formed on each sensitive mass block by using a body silicon processing technology; a plurality of pairs of fixed comb teeth are correspondingly formed on the supporting frame body to form a pair of differential capacitors serving as sensitive capacitors; and the differential comb tooth capacitors in different directions generate a differential capacitance change in response to the acceleration signals in the corresponding directions.

The invention relates to a small-target surface vertical target dispersion photoelectric measurement device. The traditional contact measurement method has the problems that the labor intensity is high, the measuring results are greatly influenced by manual work, and particularly repeated holes cannot be identified and the measuring results cannot be provided in real time. A laser light source assembly is arranged symmetrically on the lateral surface of a linear array CCD camera, an emergent main axis of the laser light source assembly is coplanar with a main axis of the linear array CCD camera, and an original-direction reflecting film is opposite to the linear array CCD camera and vertical to a light curtain sent by the laser light source assembly. The device adopts an integrated structural design, forms the light curtain converged relative to the CCD camera by matching the laser assembly of a small-power semiconductor laser and the low-cost original-direction reflecting film, reliably acquires the information of positions where bullets pass through the light curtain, effectively solves the problems of unstability and low sensitivity of light used indoors during a CCD convergence test, and has the advantages of convenient installation and debugging, simple structure, and high safety of personnel and equipment.

The invention provides a kit suitable for rapidly detecting AMH and INHB by using a double-tagging time resolution fluorescence immunoassay method. The kit comprises (1) a solid phase carrier coated by an AMH coating antibody and an INHB coating antibody; (2) a mixed calibration product of AMH and INHB; (3) an AMH detection antibody marked by using a lanthanide element 1; (4) an INHB detection antibody marked by a lanthanide element 2; (5) an immunoreaction accelerant liquid; (6) a concentrated washing liquid; and (7) a reinforcing liquid. The invention further provides a use method of the kit for rapidly detecting AMH and INHB by using the double-tagging time resolution fluorescence immunoassay method. By adopting a double-tagging time resolution fluorescence immunoassay technique, the defect that conventional AMH and INHB respectively need a single kit, that is, one kit can be only used for detecting AMH or INHB can be overcome, the detection steps can be reduced, the sampling time can be shortened, and the use amount of a blood sample can be reduced.

The present invention relates to a photosensitive composition, which is capable of being irradiated with high energy beam having a wave length of 1 to 300 nm band. The photosensitive composition includes a binder resin; and a photoelectron absorbent, capable of being excited with photoelectron emitted from the binder resin that absorbs the high energy beam, when the binder resin is irradiated with the high energy beam.

On a conventional T-shaped glass chip, a permanent magnet is utilized to fix and decorate a nanometer magnetic bead of a DNA probe in a separation channel of a chip; the rapid, sensitive, high-specific, high-stability, low-cost separation detection on the DNA is realized by utilizing electric drive sampling and combining a laser induced fluorescence detector or an electrochemical detector, thus solving the defects that the time of separating and detecting the DNA by utilizing a microfluidic chip is long, the separation effect is poor, the sensitivity is low, the specific is poor and the stability is poor and the like; the interface manufacturing is avoided by an electric sampling manner; the influence of dead volume is removed; and the sample use amount is reduced in a narrower and uniform micro pipeline, and meanwhile the lower detection limit is ensured.

The invention discloses a flexible piezoelectric three-dimensional tactile sensor array which comprises a PDMS semispherical projection layer, an upper electrode layer, a nanometer structure piezoelectric film layer, a lower electrode layer and a printed circuit board flexible substrate layer, wherein the PDMS semispherical projection layer, the upper electrode layer, the nanometer structure piezoelectric film layer, the lower electrode layer and the printed circuit board flexible substrate layer are successively connected from top to bottom. The nanometer structure piezoelectric film layer isa nano structure ZnO piezoelectric film and is arranged between the upper electrode layer and the lower electrode layer which are arranged in an array pattern manner, thereby forming a plurality of piezoelectric sensitive units. The three-dimensional tactile sensor array is composed of M*N separated sensor units. Each sensor unit comprises a PDMS semispherical projection and three piezoelectric sensitive capacitors. The PDMS semispherical projection transmits a three-dimensional contact force to the three piezoelectric sensitive capacitors. Through the magnitude of charges which are generatedby the three piezoelectric sensitive capacitors, the external three-dimensional contact force is measured. The invention further discloses a preparation method of the flexible piezoelectric three-dimensional tactile sensor. The flexible piezoelectric three-dimensional tactile sensor array has advantages of three-dimensional contact force measurement, high sensitivity, high flexibility and high dynamic response.

The invention relates to a flexible nanofiber-based electronic skin as well as a preparation method, and belongs to the field of flexible sensors. The flexible nanofiber-based electronic skin is characterized in that the electronic skin is prepared by attaching two layers of flexible nanofiber membranes which are treated by conductive treatment, and the thickness of the flexible nanofiber membrane is 30-500[mu]m, wherein at least one surface of the two surfaces which are attached is provided with micro groove or micro protrusion structures. The electronic skin solves the problems of high working voltage, large power consumption and low sensitivity in prior art; the electronic skin has the effects of sensitive layers and conductive electrodes with a single structure and a simple process, and the electronic skin can be used for industrial production; the electronic skin has good biocompatibility and can be well integrated with a human skin, and is convenient for constructing a wearable device; a unique nanostructure is applied to the electronic skin, so that the electronic skin has higher sensitivity and better stability; and the whole device is light, small, smart and is convenient for carrying.

The invention provides a microsatellite unstable site screening and analysis model construction method and device. The method comprises the following steps of: S1, taking a microsatellite site of a reference genome as a candidate MS site, S2, selecting a plurality of samples with known microsatellite instability detection state information as training set samples, carrying out mutation detection on candidate MS site areas of the training set samples, respectively recording mutation types and numbers, and calculating information entropy of mutation of each candidate MS site, and S3, correlatingthe microsatellite instability detection state information with the entropy of the information entropy of the mutation of each candidate MS site, selecting an entropy threshold of each candidate MS site for distinguishing the microsatellite instability detection result, and screening out the candidate MS site with a high distinguishing degree as an MSI site. According to the scheme, the problemsof low detection efficiency caused by excessive fixed MSI sites and low detection specificity and sensitivity caused by incapability of covering all types of samples in the prior art are solved.

A system and method for providing a virtual reality game controller with improved functionality by providing capacitive touch and proximity sensors on the controller to enable additional feedback to the user such that interaction with a physical object such as a game controller may be translated into interaction with a virtual tool in a virtual environment such as providing a visual indication in the virtual environment that a finger or thumb is approaching a button of a physical game controller.

The invention provides a light current reading circuit and a gain self-adaption light intensity imaging array circuit and a control method of the light current reading circuit and the self-adaption light intensity imaging array circuit, relates to the micro electronic technique, and solves the problems that the existing light intensity detection and automatic adjustment methods are inaccurate and slow. According to the technical scheme, the light current reading circuit is composed of a light current reading circuit, a variable gain reading circuit, an integrating capacitor resetting signal input end, a light intensity detection module, light intensity detection result reading switch modules, an integrating capacitor control end and a light intensity detection result output bus, wherein the variable gain reading circuit comprises an adjustable integrating capacitor module, the light intensity testing module comprises an integrating voltage comparison signal input end, at least one prestore comparison module, time delay units which are 1 less than the prestore comparison modules in number, and the light intensity testing result reading switch modules correspond to the number of prestore comparison modules. The light current reading circuit and the self-adaption light intensity imaging array circuit and the control method of the light current reading circuit and the self-adaption light intensity imaging array circuit have the advantage that quick and accurate light intensity test can be achieved, and are suitable for the light current reading circuit and the light intensity imaging array circuit.

The invention relates to a detection method of soluble ST2. The method comprises the steps as follows: a specific antibody of the soluble ST2 and soluble ST2 ligand protein are combined to serve as detection substances, wherein the ST2 ligand protein is IL-33 (interleukin-33) and analogues thereof. Besides, the invention further provides a kit for detection the soluble ST2 with the method. According to the detection method and the kit for soluble ST2, the IL-33 and analogues thereof can be obtained through gene engineering expression or chemical synthesis, the production process is easier to standardize, and the detection accuracy is further improved.

The invention relates to a mass spectrum qualitative and quantitative analysis method for free fatty acids based on a double-derivatization technology. The mass spectrum qualitative and quantitative analysis method comprises the following steps: 1) a photochemical derivatization reaction of double bonds of unsaturated fatty acids; 2) an N,N-diethylethylenediamine derivatization reaction of carboxyl terminals of the fatty acids; 3) a qualitative method for precise structures of the fatty acids; and 4) a quantitative method for the fatty acids. The method disclosed the invention is used for precise qualitative and quantitative analysis of the free fatty acids in actual samples, so that the problems that double-bond positions of the unsaturated fatty acids, particularly polyunsaturated fattyacids, are difficult to authenticate and the fatty acids are low in ionization efficiency and poor in detection sensitivity under a negative ion mode are solved, and high-sensitivity and accurate qualitative and quantitative analysis for the free fatty acids in the actual samples is realized.

The invention discloses a method for detecting mite allergen specific antibody in blood serum. The method comprises the following steps of: coupling mite allergen with surface carboxyl modified magnetic microspheres to prepare immunomagnetic microspheres; and mixing and incubating the blood serum to be detected and the immunomagnetic microspheres in holes of an elisa (enzyme-linked immunosorbent assay) plate to combine mite allergen with mite allergen specificity IgE in the blood serum, and detecting by adopting an enzyme-linked immunosorbent method after the elisa plate is washed. In the method disclosed by the invention, a mite allergen is directly coupled onto the surfaces of magnetic microspheres, thus all the impurities except the mite allergen specific antibody in the blood serum can be conveniently removed; and targeted mite allergen specificity antibody detection is carried out, detection time is shortened, and sensitivity, specificity and accuracy of detection are improved. The mite allergen immunomagnetic microspheres prepared by the invention can be used in standard automatic detection to improve detection efficiency.

The invention discloses a single-mass-block three-axis MEMS accelerometer with three frame structures. The single-mass-block three-axis MEMS accelerometer with the three frame structures comprises the three frame structures, namely a suspension inner frame, a suspension outer frame and a fixed frame, wherein the fixed frame is in sealed connection with a base through a bonding method, and the three frame structures are arranged symmetrically in a rectangular shape or an annular shape. The single-mass-block three-axis MEMS accelerometer with the three frame structures further comprises elastic sensing beams arranged in the X-axis direction, the Y-axis direction and the Z-axis direction respectively, wherein a varistor on the sensing beam in the X-axis direction is arranged on a bridge arm of a bridge in a series-connection mode, the variation of the varistor on the sensing beam in the X-axis direction can reduce coupling with the Y axis and the Z axis, the variation of a varistor on the sensing beam in the Y-axis direction can reduce coupling with the X axis and the Z axis, and the variation of a varistor on the sensing beam in the Z-axis direction can reduce coupling with the X axis and the Y axis. The single-mass-block three-axis MEMS accelerometer with the three frame structures is ingenious in design, is high in product yield, and resolves the problems that a single-mass-block three-axis MEMS accelerometer in the prior art is too large in inter-axis coupling, low in sensitivity and low measurement accuracy.

The invention discloses an ultrasonic testing special probe for butt girth welding of an austenitic stainless steel pipe. The probe adopts a send-receive double crystal focusing structure, so that geometrical scattering of the inner wall of a steel pipe is reduced, and random and interfering echo caused by a wedge block is reduced; an inward concave curved surface is arranged at the bottom of a delay tapered wedge, so that the top of the inward concave curved surface is in contact with the steel pipe to be tested; a horizontal polarized shear wave is generated in the austenitic stainless steel pipe by the special probe and cannot be converted into other types of waves when compared with other wave forms and reflected from the surface parallel to the polarization direction; random echo is less; the horizontal polarized shear wave is almost entirely transmitted into the weld metal, and the acoustic beam directional deflection is minimum when compared with other wave forms. The ultrasonic testing special probe can solve the technical problems that deficiencies are found in the sensibility, the signal to noise ratio and the resolution of ultrasonic testing for butt girth welding of small caliber austenitic stainless steel pipes with phi 34 mm to phi 168 mm external diameter and 3.5 mm to 20 mm wall thickness.

The invention discloses a method for producing a high-sensitivity surface reinforcement Raman sensor chip. The method comprises the following steps: (1) carrying out hydrophilic treatment on a substrate; (2) selecting uniform medium nanosphere latex solution, and a self-assembling a single layer of densely arranged medium nanosphere array on the substrate subjected to the hydrophilic treatment by using a spin coating method or a puling method; (3) utilizing a reactive ion etching technology to etch the medium nanosphere array which is obtained in the step 2; and (4) coating a metal film on the etched medium nanosphere array which is obtained in the step 3, thus obtaining the high-sensitivity surface reinforcement Raman sensor chip, wherein the thickness of the coating film is smaller than the etching thickness in the step 3,. The method is used for solving the contradiction among repeatability, uniformity and sensitivity of the conventional surface reinforcement Raman sensor chips which are produced through a physical etching method and a chemical synthesizing method, and the produced surface reinforcement Raman sensor chip has high sensitivity and good repeatability and uniformity.

The invention discloses a doping silylene based MEMS piezoresistive pressure sensor and a manufacturing method thereof. According to one method, an insulating layer is deposited at the surface of a substrate based on a surface silicon processing technology, and the insulating layer is etched so as to form a cavity; silylene is deposited on Ag, and the cavity is completely covered; the Ag substrate is peeled off, and then anti-oxidation protection and doping are carried out on a silylene film; and finally, two metal electrodes are deposited at the edge above the film, and each electrode is welded with a wire. According to another method, a mode of a traditional piezoresistive pressure sensor is adopted, wherein the traditional piezoresistive pressure sensor comprises glass, a substrate, an insulating layer, a silylene film, an anti-oxidation protection layer and metal electrodes from the bottom up. The doping silylene based MEMS piezoresistive pressure sensor has the advantages of good compatibility between silylene and the traditional semiconductor technology, simple manufacturing process, higher sensitivity and wider application.

The invention discloses a method for detecting allergen-specific antibodies in serum. The method comprises the following steps: coupling anti-human IgE antibodies with carboxyl surface modified magnetic microballoons to obtain immunomagnetic microspheres; incubating the immunomagnetic microspheres with serum to be measured so as to enable the immunomagnetic microspheres to bind to IgE in the serum to be measured; carrying out magnetic separation to obtain immunomagnetic microsphere-IgE conjugates, dissolving deposition of the magnetic separation in a buffer solution, adding the mixed solution into apertures of an enzyme label plate which is coated with allergen, and carrying out detection by the ELISA adsorption method after incubation and plate washing. According to the invention, magnetic microballoons are coupled with anti-human IgE antibodies to prepare immunomagnetic microspheres which are mixed with serum to be measured for incubation and are bond to all the IgE in serum; the immunomagnetic microspheres are enriched and IgE is separated; the ELISA adsorption method is employed to detect whether there is specific IgE bond to allergen in serum. The method provided in the invention enables all the IgE to be separated from serum through immunomagnetic microspheres, impurities to be removed, and sensitivity, specificity and accuracy of ELISA adsorption detection to be improved.

The invention relates to a gear double-face engagement precise detection device. For a common double-engagement instrument, a handwheel is shaken to enable a leading screw to rotate to drive a slide block to do longitudinal movement on a linear guide rail on a support for engagement and separation of a detected gear and a standard gear, the structure is complex, and the cost is relatively high. The gear double-face engagement precise detection device comprises a support, wherein the support is provided with a measuring assembly, a detected assembly, a position adjusting device and a measuring assembly. The gear double-face engagement precise detection device is characterized in that the detected assembly comprises a swinging shaft and a swinging frame arranged on the swinging shaft horizontally, the position adjusting device comprises a compaction spring, a cam with a handle and a mobile board, the mobile board is movably arranged on the support, the compaction spring and the cam with the handle are mounted in mounting holes of the mobile board, the measuring assemblies comprise displacement sensors arranged on the mobile board, and axial lines of the displacement sensors are perpendicular to the swinging frame. The gear double-face engagement precise detection device has a simple structure, is simple and convenient to operate and is suitable for mass detection and quality control in a gear production field and a detection center.