The invention discloses a rapid propagation method of ginger virus-free seedlings by one step. The rapid propagation method comprises the following steps: A, selecting tender shoots germinating on ginger rhizomes as explants; B, carrying out heat treatment to the explants, so as to detoxify the explants; C, disinfecting the detoxified explants with ethyl alcohol and mercury bichloride; D, peeling stem tips by a dissecting microscope; E, inducing the stem tips to grow micro ginger cluster buds under light condition and constant temperature condition; F, carrying out virus detection on the micro ginger cluster buds by an enzyme-linked immunosorbent assay (ELISA); G, carrying out subculture multiplication culture on the virus-free micro ginger cluster buds; H, transplanting virus-free micro ginger cluster buds with roots after plant division. By the utilization of the rapid propagation method, various viruses and germs in ginger bodies are removed effectively, germ-free and virus-free ginger seedlings are cultured, virus-free healthy cultivation of the ginger is realized, space occupation of propagation is small, propagation speed is high, one virus-free bud can propagate more than hundred thousand virus-free ginger seedlings for one year, a problem of virus-free ginger seedling supplying in plantation of ginger is solved effectively, good quality of the ginger is recovered, the disease occurrence is reduced, and the output and quality of the ginger are improved.