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Application of bacillus megaterium in degradation of peanut meal aflatoxin B1

A technology of Bacillus megaterium and aflatoxin, applied in the field of applied microorganisms, to achieve the effects of less environmental pollution, less harm to humans and animals, and low energy consumption

Inactive Publication Date: 2011-05-25
JIANGNAN UNIV
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  • Description
  • Claims
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Problems solved by technology

At present, there is no research report at home and abroad that Bacillus megaterium can degrade aflatoxin B in solid-state fermentation of peanut meal. 1

Method used

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  • Application of bacillus megaterium in degradation of peanut meal aflatoxin B1

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Embodiment 1

[0012] Degradation of aflatoxin B in peanut meal by Bacillus megaterium 1 The specific operation process is as follows: culture Bacillus megaterium in LB solid medium at 37°C for 24 hours, pick a single colony, insert it into LB liquid medium, and cultivate it on a shaking table at 37°C at 180r / min; buy 10mg from Fermentek Company aflatoxin B 1 Dissolve in 50mL benzene-acetonitrile (98:2, V / V) solution, the concentration is 200μg / mL, that is, aflatoxin B 1 Stock solution, take a certain amount of aflatoxin B 1 stock solution, with N 2 Blow, volatilize solvent benzene and acetonitrile, add a certain proportion of phosphate buffer saline (PBS, pH7.4), make aflatoxin B 1 The final concentration is 2000ng / mL; crush the peanut meal and pass through a 20-mesh sieve; weigh 5g of peanut meal (accurate to 0.01g) in a petri dish, wrap it in kraft paper, and sterilize by high-pressure steam at 121°C for 20 minutes; sterilize the peanut meal After cooling, add 250 μl of aflatoxin B at...

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Abstract

The invention provides application of bacillus megaterium in degradation of peanut meal aflatoxin B1, and belongs to the technical field of microbe application. A method comprises the following steps of: sterilizing crushed target peanut meal, adding a certain amount of aflatoxin B1, then adding bacillus megaterium liquid in an amount which is 5 to 10 percent of the inoculation amount, adding sterile water in a ratio of materials to water of 1:0.5-1.5, and fermenting for 48 to 72 hours at the temperature of between 30 and 40 DEG C; and adding a solvent into the peanut meal after fermentation is completed, shaking a table for 10 to 50 minutes, extracting the residual aflatoxin B1, and detecting the content of the aflatoxin B1 by using enzyme linked immunosorbent assay. The method has the advantages of capability of effectively reducing the aflatoxin B1 content of the peanut meal, low energy consumption, low environmental pollution and no need of wastewater treatment, strict sterile operation or continuous ventilation.

Description

technical field [0001] The invention relates to a strain of Bacillus megaterium used in degrading peanut meal aflatoxin B 1 The application in the invention belongs to the field of applied microbiological technology. Background technique [0002] Peanut meal is a by-product of extracting oil from shelled peanuts. The nutritional value of peanut meal is higher, and the content of metabolizable energy is higher than that of soybean meal, but at present, peanut meal is mainly used as feed, and the price is low. One of the main reasons for limiting the price and wide application of peanut meal is that it is susceptible to Aspergillus flavus and produces aflatoxins, among which aflatoxin B 1 most representative. [0003] Aflatoxin B 1 The removal methods mainly include physical, chemical and biological methods. The biological detoxification method has mild treatment conditions and can increase the nutritional value of the product, which has received widespread attention. Re...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23K1/14C12N1/20C12S3/00C12R1/11A23K10/30A23L5/20
Inventor 金青哲孙丰芹王兴国
Owner JIANGNAN UNIV
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