167 results about "Bacillus megaterium" patented technology

According to the invention, the monooxygenase CYP102A1 from Bacillus megaterium is subjected to system modification and optimization, a series of monooxygenase mutants with improved activity and improved debenzylation selectivity are obtained, the activity of the mutant with the optimal effect is improved by 93.4 times compared with that of wild type monooxygenase CYP102A1, and the selectivity is improved to 99.5% from 33.2%. The monooxygenase mutant can be applied to catalytic synthesis of 3R, 5S-dihydroxy compounds, synthesis steps are simplified, reaction selectivity is improved, reaction conditions are mild, the monooxygenase mutant is environmentally friendly and efficient, and the overall efficiency of statin drug synthesis can be improved.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The present invention relates to the technical field of microbial deodorants, and discloses a microbial composite deodorant and a preparation method thereof. The microbial composite deodorant comprises a bacterial powder mixture, which is embedded in a load material, and the load material is externally wrapped with a protective agent. The bacterial powder mixture comprises lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum, wherein the mass ratio of lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum is 1:1-3:1-2:0.5-1:1-2. The present invention adopts the above-mentioned microbial composite deodorant and a preparation method thereof. The prepared microbial composite deodorant can significantly remove malodorous gases such as NH3 and H2S in biological feces, has a long action time, and has a significant effect. At the same time, the raw materials of the microbial composite deodorant are green and safe, reducing health problems caused by accidental ingestion by livestock.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to bacillus megatherium XW-02 (the preservation number is CCTCC NO: M 2025185), a microbial preparation of the bacillus megatherium XW-02, and application of the microbial preparation. The bacillus megatherium XW-02 has the advantages that the preservation number is CCTCC NO: M 2025185; the strain can efficiently express xylanase through xylan induction, hemicellulose in tobacco leaves is remarkably degraded, chemical composition is optimized, and combustion harmful substances are reduced. The treated cigar has the advantages that the aroma richness is improved, the purity is improved, the taste mellowness is improved, and the overall quality is improved by 1-2 grades. The xylan extracted from agricultural wastes is used as an inducer to construct a green biological manufacturing system, the cost is reduced by more than 80% compared with the traditional process, and the emission of three wastes is reduced. The process compatibility is high, the method can be matched with an existing production line only by additionally arranging a strain culture module, the single-line annual tobacco leaf treatment amount reaches 500 tons, and economic benefits are remarkable. The invention provides a safe, efficient and environment-friendly quality improvement solution for the tobacco industry, and has great industrialization value.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention belongs to the technical field of microbial fermentation and biological agriculture, and discloses a rice straw decomposition agent as well as a preparation method and application thereof. The rice straw decomposition agent is obtained by adopting bacillus megatherium (Bacillus megatherium) Z101 to be subjected to fermentation culture after activation of the bacillus megatherium Z101. The application method comprises the steps that harvested rice straw is flatly laid in a rice field, the water level of the rice field is kept to submerge the rice straw, then the rice straw decomposition agent is diluted with water and then sprayed to the surface of the laid rice straw for decomposition fermentation treatment, and decomposed rice straw is obtained. The rice straw decomposing inoculant is a fermentation culture solution of bacillus megatherium Z101, and compared with a fermentation culture solution obtained by conventional bacillus megatherium in the market, the rice straw decomposing inoculant has higher rice straw decomposing efficiency and speed, in-situ decomposing and field returning of rice straw are facilitated, and a new application way is provided for the bacillus megatherium Z101.

The invention relates to a method for repairing light rare earth and heavy metal compound polluted soil, which is characterized in that the compound polluted soil is repaired by adopting bacillus sp., the bacillus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22085, and the preservation date is March 29, 2021. The repairing process comprises the following steps: mixing the bacillus subtilis bacterial liquid with the cementing liquid to obtain a mixed liquid; mixing the air-dried polluted soil with the mixed solution, and filling the mixture into a mold for reaction; the mold is communicated with a grouting pipe to inject the mixed liquid, and multi-round pumping is carried out; and after pumping is completed, the polluted soil is maintained and naturally air-dried. According to the method, dominant strains are adopted, biological adsorption and biological mineralization are combined, and effective control over the light rare earth and heavy metal compound contaminated soil is achieved under the action of dual bioremediation.

The invention discloses application of bacillus megaterium capable of synthesizing indole-3-lactic acid to promotion of soybean growth and nodulation, the bacillus megaterium has the characteristic of promoting growth of various plants, and the fresh weight and nodulation of soybeans are remarkably improved; a large amount of indole-3-lactic acid (ILA) is synthesized and secreted, and the fresh weight and nodulation of soybean plants are remarkably improved; the strain has a good application prospect as a biological inoculant for leguminous crops.

The invention discloses a microbial soil conditioner for saline-alkali soil. The microbial soil conditioner is prepared from the following raw materials in parts by mass: 5-20 parts of microbial flora, 60-85 parts of a carrier material, 5-15 parts of a functional additive and 3-8 parts of a water-retaining agent, wherein the microbial flora comprises halotolerant bacteria, phosphate solubilizing bacteria, nitrogen-fixing bacteria and potassium bacteria, and the volume ratio of the halotolerant bacteria to the phosphate solubilizing bacteria to the nitrogen-fixing bacteria to the potassium solubilizing bacteria is (2-4): (1-3): (1-2): (1-2). Wherein the halotolerant bacteria are bacillus subtilis, the phosphate solubilizing bacteria are bacillus megatherium, the nitrogen-fixing bacteria are brown nitrogen-fixing bacteria, and the potassium solubilizing bacteria are bacillus mucilaginosus. The invention relates to the technical field of agricultural soil improvement, in particular to a microbial soil conditioner for saline-alkali soil, which has the advantages of reducing the pH value and salt content of soil, improving the soil structure, promoting the growth of crops and protecting the environment due to the adoption of the technical scheme.

The invention belongs to the technical field of microbial fermentation and biological agriculture, and discloses a soil remediation fungicide as well as a preparation method and application thereof. The preparation method comprises the following steps: activating bacillus megatherium Z101, inoculating the activated bacillus megatherium Z101 into fermentation liquor containing rice straw powder, carrying out fermentation culture, and carrying out vacuum spray drying on fermentation mixed liquor after fermentation is completed, so as to obtain soil remediation bacterium powder; or further mixing the soil remediation bacterium powder with auxiliary materials and water, and granulating to obtain the soil remediation bacterium granules. The application method comprises the following steps: in a rice planting stage, applying the soil remediation fungicide and an inorganic fertilizer together to remedy rice field soil and promote rice growth. The soil remediation fungicide prepared by fermentation culture of the bacillus megatherium Z101 has remarkable advantages in the aspects of rice yield increase, soil pH value increase and physicochemical property improvement, and a new application way is provided for the bacillus megatherium Z101.

The invention discloses a microbial agent for promoting sand fixation in sand land and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises bacillus megatherium, pseudomonas fluorescens, lactobacillus mucus, trichoderma harzianum and azotobacter chroococcum which are mixed in the form of bacterial liquid, the volume ratio of the bacterial liquid is (3-5): (2-3): (2-3): (1-2): (1-2), and all bacterial strains have a synergistic effect. When the microbial agent is applied to soil, the soil stability is obviously improved after the microbial agent is applied, the water retention rate of sand land soil can be improved by 15.9 times within 10 days, the sand rising wind speed reaches 18.5 m / s and above, and the vegetation coverage can reach 32% and above after one year. The microbial agent disclosed by the invention is simple and convenient in preparation method, can remarkably promote sand fixation in the sand land and improve the survival rate of plants in the sand land, has the characteristics of environmental protection and high efficiency, and is easy to popularize and apply.

The invention belongs to the technical field of cortex acanthopanacis planting, and particularly relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method. The invention relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method, which comprises the following steps: S1, selecting a low-hill mountain land with the altitude of 200-400m and the gradient of lower than 15 degrees according to the tree age of 3-5 years and the canopy density of 0.25-0.35; s2, drip irrigation pipes are buried after soil preparation, then cortex acanthopanacis cutting slips are cut, clear water is poured thoroughly, and microbial water and fertilizer are dropped through the drip irrigation pipes in the subsequent planting process; wherein the microbial water fertilizer is formed by mixing bacillus safensis fermentation liquor and bacillus megatherium fermentation liquor. According to the method, in the under-forest cortex acanthopanacis integrated planting process, microbial water and fertilizer are subjected to drip irrigation, the prevention and treatment effect on cortex acanthopanacis root diseases can be effectively prevented and improved, and development of under-forest cortex acanthopanacis integrated planting is promoted.

The invention discloses a bacillus megaterium-containing microbial fertilizer for soil improvement and a preparation method thereof, and belongs to the technical field of fertilizers, the bacillus megaterium-containing microbial fertilizer for soil improvement is prepared from the following raw materials in parts by weight: 35-50 parts of an immobilized microbial agent, 3-5 parts of a water-retaining agent, 10-15 parts of humic acid and 2-5 parts of trehalose; the immobilized microbial agent is amino MOF (Metal Organic Framework) modified charcoal loaded bacillus megaterium; the microbial fertilizer is prepared from the immobilized microbial agent, the water-retaining agent, humic acid and trehalose, is high in pertinence and outstanding in performance in soil improvement, on one hand, available phosphorus in reclamation soil is activated, needed nutritional ingredients can be provided for plants, on the other hand, soil organic matter can be activated and updated, and the soil fertility is improved. The soil fertility is improved and the plant nutrient requirements are met.

The invention belongs to the technical field of microbial remediation, and particularly relates to bacillus megatherium 1-9-1, a microbial agent and application of the bacillus megatherium 1-9-1 and the microbial agent. The bacillus megaterium 1-9-1 is preserved in the China General Microbiological Culture Collection Center on December 21, 2023, and the preservation number of the bacillus megaterium 1-9-1 is CGMCC (China General Microbiological Culture Collection Center) No.29373. According to the application of the bacillus megatherium 1-9-1 in the aspects of repairing the overburden cracks and preventing the gas layer from escaping, the bacillus megatherium 1-9-1 is attached to the surface of the rock, carbonate precipitates are mineralized and deposited around the rock pores and the cracks for repairing, and meanwhile CO2 is absorbed and sealed. After the microbial agent provided by the invention is injected into a cover layer, the metabolite of the microbial agent can block pores and tiny cracks in the cover layer, so that the purposes of better carbon sequestration effect and oil and gas reservoir protection are achieved.

The invention relates to the technical field of biology, and particularly discloses bacillus megaterium LLH-B2 and application thereof. The invention relates to bacillus megaterium LLH-B2, which is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on November 14, 2022, and is named as bacillus megaterium LLH-B2, and the preservation number is CCTCC NO: M 20221787. The bacillus megaterium LLH-B2 is named as bacillus megaterium LLH-B2. The bacillus megaterium LLH-B2 can stably survive for a long time in a sandy soil drought environment, heavy metal treatment of sandy soil is efficiently treated, meanwhile, the soil fertility is improved, and plant growth is promoted.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

The invention relates to the technical field of microbial fertilizers. The invention aims to provide a fruit and vegetable biological source bacterial fertilizer system which comprises the following raw materials in parts by weight: 600 parts of wormcast, 200 parts of straw powder, 100 parts of humic acid, 1 part of a microbial agent and 5 parts of brown sugar. The microbial agent is prepared from bacillus subtilis, bacillus megatherium and actinomycetes according to the weight ratio of 5: 3: 2. According to the invention, the natural wormcast is used as a microbial carrier, and by virtue of the unique physical structure and microbial basis of the wormcast and in combination with a functional strain compounding technology, the defects of the traditional fertilizer can be effectively overcome.

Provided herein are screening methods and animal models relevant to ocular diseases such as age-related macular degeneration (AMD), e.g., for use in identifying candidate therapeutics for treating or preventing ocular diseases such as AMD. Also provided herein are compounds / compositions useful for killing or inhibiting the growth of microorganisms such as Bacillus megaterium. Further provided herein are methods of using the compounds / compositions to treat infections with microorganisms such as Bacillus megaterium, as well as to treat or prevent diseases or conditions associated with such infections, such as AMD.

The invention discloses a preparation method of a microbial complex microbial inoculant and a Chinese herbal medicine complex microbial inoculant and application of the microbial complex microbial inoculant in offshore fish culture, and belongs to the technical field of microbial engineering. The composite microbial inoculant comprises bacillus megatherium and rhodotorula mucilaginosa. In addition, the invention also provides a preparation method of the Chinese herbal medicine complex microbial inoculant, which comprises the following steps: mixing the microbial complex microbial inoculant, the Chinese herbal medicine and the base solution, and fermenting. In addition, the invention also provides a Chinese herbal medicine complex microbial inoculant prepared by the preparation method. In addition, the invention also provides a feed, and the mass addition amount of the compound microbial inoculant prepared by the preparation method in the feed is 5%-10%. In addition, the invention also provides an application of the Chinese herbal medicine complex microbial inoculant prepared by the preparation method in offshore fish culture. The microbial complex microbial inoculant can be used for Chinese herbal medicine fermentation, and the problem of obesity of fishes caused by artificial sweeteners can be effectively solved after the fishes are fed with the microbial complex microbial inoculant.

The application discloses a method for repairing light rare earth and heavy metal composite contaminated soil, and adopts bacillus megaterium (Bacillus megaterium) to repair the composite contaminated soil. Oceanobacillus sp. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No.22085, and is preserved on March 29, 2021. The repairing process is as follows: mixing bacillus megaterium liquid and cementing liquid to obtain a mixed liquid; mixing the air-dried contaminated soil and the mixed liquid, and then loading the mixed liquid into a mold for reaction; connecting a grouting pipe to the mold to inject the mixed liquid, and performing multiple rounds of pumping; after the pumping is completed, the contaminated soil is maintained and air-dried naturally. The application adopts the advantage strain, combines biological adsorption and biological mineralization, and realizes the effective control of the light rare earth and heavy metal composite contaminated soil under the action of double biological repair.

The invention belongs to the field of animal feeding, and particularly relates to application of bacillus megatherium in preparation of fermented feed for reducing methane emission of mutton sheep, the bacillus megatherium is preserved in the China General Microbiological Culture Collection Center on December 6, 2004, and the preservation number is GMCCNo.1259. The invention also provides a fermented feed for reducing methane emission of mutton sheep, the degradation rate of straw rumen dry matter and crude protein can be remarkably increased, the ammonia nitrogen content in the feed is remarkably reduced, and the lactic acid bacteria content is remarkably increased.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The invention provides a compound microbial agent for preventing diseases, promoting growth and resisting salt and alkali of alfalfa as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of biocontrol microbial agents. The compound microbial agent disclosed by the invention is prepared from bacillus mojavensis Bmo-MFL22, bacillus halophilus Bh-MFL68, bacillus megatherium Bme-MFL102, bacillus circulans Bc-MFL233 and bacillus subtilis Bs-MFL379, and the compound microbial agent disclosed by the invention is prepared from bacillus mojavensis Bmo-MFL22, bacillus halophilus Bh-MFL68, bacillus megatherium Bme-MFL102, bacillus circulans Bc-MFL233 and bacillus subtilis The compound microbial agent disclosed by the invention has a prevention and treatment effect on alfalfa root rot. The strains in the compound microbial agent disclosed by the invention have relatively good compatibility, and can synergistically promote the growth of leguminous crops such as alfalfa, peanuts and soybeans, so that the crop yield is increased. The compound microbial agent disclosed by the invention can also improve rhizosphere soil of leguminous crops and reduce the salt and alkali content in the soil.

The invention relates to a preparation for preventing and / or treating sugarcane leukosis and application thereof, and relates to the technical field of biological agents. The preparation comprises bacillus mucilaginosus, bacillus megatherium, bacillus laterosporus, alternaria tenuissima activator protein, calcium acetate, magnesium sulfate and glucose. The preparation has a relatively good prevention and / or treatment effect on the sugarcane bacterial leaf streak, and also can promote the growth of the sugarcane at the same time.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention provides a method for synthesizing melanin nanoparticles by using escherichia coli, and belongs to the technical field of biological genetic engineering. According to the method, a bacillus megaterium-derived tyrosinase gene Tyr1 (SEQ ID NO.2) after codon optimization is taken as a target gene, a recombinant expression plasmid pET-24a-Tyr1 is constructed, and the recombinant expression plasmid pET-24a-Tyr1 is transformed into an escherichia coli BL21 (DE3) competent cell to obtain the genetically engineered bacterium. After the engineering bacterium induces expression of tyrosinase through IPTG (isopropyl-beta-d-thiogalactoside), polymerization of an L-tyrosine substrate is catalyzed in the presence of CuSO4 to obtain high-purity melanin nanoparticles. The melanin nano-particles synthesized by the method are uniform in particle size, have wide-spectrum strong absorption characteristics in a wavelength range of 400-800nm, show excellent photo-thermal performance, free radical scavenging capacity and biocompatibility, and have wide application prospects in the fields of biomedicine and functional materials.

The invention relates to the technical field of microbial carbon sequestration, and discloses bacillus megaterium, a carbon sequestration microbial agent as well as a preparation method and application of the carbon sequestration microbial agent. The preservation number of the bacillus megaterium provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35586. The bacillus megaterium provided by the invention is a new strain of bacillus megaterium separated from a water sample in an oil field. The bacillus megaterium can effectively immobilize carbon dioxide in the atmosphere and generate gases such as nitrogen under a high-temperature condition through a unique metabolic pathway, so that the synergistic effect of carbon sequestration and gas production is realized, and the energy recovery rate and environmental benefits of the whole biological process are improved.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.