108 results about "Bacillus megaterium" patented technology

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to a method for repairing light rare earth and heavy metal compound polluted soil, which is characterized in that the compound polluted soil is repaired by adopting bacillus sp., the bacillus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22085, and the preservation date is March 29, 2021. The repairing process comprises the following steps: mixing the bacillus subtilis bacterial liquid with the cementing liquid to obtain a mixed liquid; mixing the air-dried polluted soil with the mixed solution, and filling the mixture into a mold for reaction; the mold is communicated with a grouting pipe to inject the mixed liquid, and multi-round pumping is carried out; and after pumping is completed, the polluted soil is maintained and naturally air-dried. According to the method, dominant strains are adopted, biological adsorption and biological mineralization are combined, and effective control over the light rare earth and heavy metal compound contaminated soil is achieved under the action of dual bioremediation.

The invention belongs to the technical field of cortex acanthopanacis planting, and particularly relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method. The invention relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method, which comprises the following steps: S1, selecting a low-hill mountain land with the altitude of 200-400m and the gradient of lower than 15 degrees according to the tree age of 3-5 years and the canopy density of 0.25-0.35; s2, drip irrigation pipes are buried after soil preparation, then cortex acanthopanacis cutting slips are cut, clear water is poured thoroughly, and microbial water and fertilizer are dropped through the drip irrigation pipes in the subsequent planting process; wherein the microbial water fertilizer is formed by mixing bacillus safensis fermentation liquor and bacillus megatherium fermentation liquor. According to the method, in the under-forest cortex acanthopanacis integrated planting process, microbial water and fertilizer are subjected to drip irrigation, the prevention and treatment effect on cortex acanthopanacis root diseases can be effectively prevented and improved, and development of under-forest cortex acanthopanacis integrated planting is promoted.

The invention relates to the technical field of biology, and particularly discloses bacillus megaterium LLH-B2 and application thereof. The invention relates to bacillus megaterium LLH-B2, which is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on November 14, 2022, and is named as bacillus megaterium LLH-B2, and the preservation number is CCTCC NO: M 20221787. The bacillus megaterium LLH-B2 is named as bacillus megaterium LLH-B2. The bacillus megaterium LLH-B2 can stably survive for a long time in a sandy soil drought environment, heavy metal treatment of sandy soil is efficiently treated, meanwhile, the soil fertility is improved, and plant growth is promoted.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

Provided herein are screening methods and animal models relevant to ocular diseases such as age-related macular degeneration (AMD), e.g., for use in identifying candidate therapeutics for treating or preventing ocular diseases such as AMD. Also provided herein are compounds / compositions useful for killing or inhibiting the growth of microorganisms such as Bacillus megaterium. Further provided herein are methods of using the compounds / compositions to treat infections with microorganisms such as Bacillus megaterium, as well as to treat or prevent diseases or conditions associated with such infections, such as AMD.

The application discloses a method for repairing light rare earth and heavy metal composite contaminated soil, and adopts bacillus megaterium (Bacillus megaterium) to repair the composite contaminated soil. Oceanobacillus sp. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No.22085, and is preserved on March 29, 2021. The repairing process is as follows: mixing bacillus megaterium liquid and cementing liquid to obtain a mixed liquid; mixing the air-dried contaminated soil and the mixed liquid, and then loading the mixed liquid into a mold for reaction; connecting a grouting pipe to the mold to inject the mixed liquid, and performing multiple rounds of pumping; after the pumping is completed, the contaminated soil is maintained and air-dried naturally. The application adopts the advantage strain, combines biological adsorption and biological mineralization, and realizes the effective control of the light rare earth and heavy metal composite contaminated soil under the action of double biological repair.

The invention belongs to the field of animal feeding, and particularly relates to application of bacillus megatherium in preparation of fermented feed for reducing methane emission of mutton sheep, the bacillus megatherium is preserved in the China General Microbiological Culture Collection Center on December 6, 2004, and the preservation number is GMCCNo.1259. The invention also provides a fermented feed for reducing methane emission of mutton sheep, the degradation rate of straw rumen dry matter and crude protein can be remarkably increased, the ammonia nitrogen content in the feed is remarkably reduced, and the lactic acid bacteria content is remarkably increased.

The invention belongs to the technical field of biological phosphorus-silicon fertilizers, and particularly relates to a liquid complex microbial inoculant capable of repairing heavy metals and activating phosphorus and silicon and a biological phosphorus-silicon fertilizer thereof. The liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria Cupriavidus and Ochrobactrum, nitrogen-fixing bacteria Azospirillum brasilense, phosphate solubilizing bacteria Bacillus megaterium and Pseudomonas, potassium solubilizing bacteria Bacillus mucilaginosus, growth-promoting bacteria Pseudomonas fluorescens, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants, microbial inoculants and microbial inoculants. The biocontrol bacteria are trichoderma harzianum and bacillus subtilis, and the synergistic bacteria are saccharomyces cerevisiae; the biological phosphorus-silicon fertilizer is prepared from the liquid complex microbial inoculant. According to the biological phosphorus-silicon fertilizer provided by the invention, through scientific compatibility of various functional strains, heavy metal remediation and soil phosphorus and silicon solubilization are realized, absorption of phosphorus and silicon in soil is promoted, and the crop yield is increased.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention provides a method for synthesizing melanin nanoparticles by using escherichia coli, and belongs to the technical field of biological genetic engineering. According to the method, a bacillus megaterium-derived tyrosinase gene Tyr1 (SEQ ID NO.2) after codon optimization is taken as a target gene, a recombinant expression plasmid pET-24a-Tyr1 is constructed, and the recombinant expression plasmid pET-24a-Tyr1 is transformed into an escherichia coli BL21 (DE3) competent cell to obtain the genetically engineered bacterium. After the engineering bacterium induces expression of tyrosinase through IPTG (isopropyl-beta-d-thiogalactoside), polymerization of an L-tyrosine substrate is catalyzed in the presence of CuSO4 to obtain high-purity melanin nanoparticles. The melanin nano-particles synthesized by the method are uniform in particle size, have wide-spectrum strong absorption characteristics in a wavelength range of 400-800nm, show excellent photo-thermal performance, free radical scavenging capacity and biocompatibility, and have wide application prospects in the fields of biomedicine and functional materials.

The invention relates to the technical field of microbial carbon sequestration, and discloses bacillus megaterium, a carbon sequestration microbial agent as well as a preparation method and application of the carbon sequestration microbial agent. The preservation number of the bacillus megaterium provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35586. The bacillus megaterium provided by the invention is a new strain of bacillus megaterium separated from a water sample in an oil field. The bacillus megaterium can effectively immobilize carbon dioxide in the atmosphere and generate gases such as nitrogen under a high-temperature condition through a unique metabolic pathway, so that the synergistic effect of carbon sequestration and gas production is realized, and the energy recovery rate and environmental benefits of the whole biological process are improved.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.

The application discloses a kind of microbial fertilizer and its in improving white pulp soil carbon sequestration capacity and application in promoting straw decomposition, belong to the technical field of microbial fertilizer.The microbial fertilizer includes organic matter, biochar, microbial inoculant, trace element and microbial activator;The microbial inoculant includes bacillus ND2 and bacillus megaterium CICC 22681;The microbial activator includes fulvic acid, gamma-polyglutamic acid and sorbitol.The microbial fertilizer of the application has the dual function of white pulp soil improvement and straw decomposition, can accelerate straw decomposition, improve white pulp soil structure, improve soil fertility and carbon sequestration capacity, help agricultural waste resourceization and soil quality improvement collaborative development, in white pulp soil area or other barren soil area in future agricultural production, it is expected to become important technical support for promoting sustainable development of ecological agriculture, and application prospect is wide.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.

The invention provides a high-activity glutamic acid decarboxylase mutant and application thereof, the glutamic acid decarboxylase mutant is obtained by performing site-specific mutagenesis on wild-type glutamic acid decarboxylase derived from bacillus megatherium, and specifically, histidine at the 466th site is mutated into threonine. Compared with wild-type glutamate decarboxylase, the glutamic acid decarboxylase mutant constructed by the invention has the advantages that the enzyme activity is improved by 50.03%, the enzyme activity is improved by 4.1% compared with other glutamic acid decarboxylase mutants, the enzyme activity of the glutamic acid decarboxylase mutant constructed by the invention is the highest under the condition that the pH is 5-6, and after the glutamic acid decarboxylase mutant is incubated for 6 hours at the temperature of 50 DEG C, the enzyme activity of the glutamic acid decarboxylase mutant is greatly improved. The glutamic acid decarboxylase mutant constructed by the invention still keeps the catalytic activity of 45.9%. In conclusion, the glutamic acid decarboxylase mutant constructed by the invention effectively improves the enzyme activity and stability of glutamic acid decarboxylase, effectively solves the technical bottlenecks of insufficient catalytic efficiency, poor thermal stability and the like of an enzyme preparation in the existing production process, and has important application value for promoting the industrial production of GABA (gamma-aminobutyric acid).

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The present application belongs to the technical field of cigar processing, and particularly relates to a method for fermenting cigar tobacco leaves by using Fujian rock tea. The finished Fujian rock tea is heated to be refluxed, filtered and concentrated to obtain a rock tea concentrate, the concentrate is sprayed into the cigar tobacco leaves to be rehydrated, fresh Fujian rock tea leaves are added, the concentrate is sprayed again to adjust the moisture of the system, carboxy bacillus and bacillus megaterium, pectinase, glucosidase and protease are added to perform microbial-enzyme synergistic fermentation, and the fermented Fujian rock tea cigar tobacco leaves are obtained through separation and re-drying. The present application creatively uses the rock tea extract concentrate to rehydrate the tobacco leaves and co-ferment the tobacco leaves with the fresh rock tea leaves, so that the rock tea flavor is fully integrated into the cigar tobacco leaves. The artificial inoculation microorganisms carboxy bacillus and bacillus megaterium and the complex enzyme preparation pectinase, glucosidase and protease suitable for co-fermentation of the Fujian rock tea and the cigar tobacco leaves are screened, and the flavor of the cigar tobacco leaves is significantly improved.

The invention relates to a biological seed coating agent and a preparation method thereof. The biological seed coating agent comprises a composite biological agent and a film-forming agent, the composite biological agent is a composite biological agent of bacillus polymyxa and bacillus megatherium. The film-forming agent comprises sodium alginate and polyvinyl alcohol; the mass ratio of sodium alginate to polyvinyl alcohol is (1-3): 2. The biological seed coating agent provided by the invention has an indoor and field control effect of more than 75% on radish black rot, has a remarkable promotion effect on growth of roots and stems of radish seedlings in an indoor test, and has a field test that compared with conventional control of farmers, the yield is increased by 1.6%, the commodity rate is increased by 3.3%, and the cost is saved and the efficiency is increased by 12%.

The application discloses a rhizosphere phosphorus-fixing and phosphorus-dissolving microbial interaction regulation method and belongs to the field of microbial agents. 2+ and Mg 2+ bridge the two-way metabolite closed-loop mechanism of Bacillus megaterium exopolysaccharide, ferment by two-step method respectively and mix in an optimized ratio, prepare a synergistic microbial agent by compounding biochar and vermiculite into a heterogeneous carrier at a mass ratio of (2-4):(1-3), and apply the synergistic microbial agent in a circular ditch during the afforestation period of Litsea cubeba seedlings. The application breaks through the traditional cognition that functional antagonistic microbial groups cannot coexist, and the phosphorus accumulation amount of Litsea cubeba plants is increased by 28% compared with single phosphorus-dissolving microbial agent treatment.

The invention provides a complex microbial inoculant for promoting acid sugarcane soil improvement as well as a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The complex microbial inoculant comprises bacillus amyloliquefaciens N15300, bacillus megatherium 2020055 and bacillus licheniformis 2020049, wherein the ratio of the effective viable count of the bacillus amyloliquefaciens N15300 to the effective viable count of the bacillus megatherium 2020055 to the effective viable count of the bacillus licheniformis 2020049 is (1.8-2.2): (1.8-2.2): (0.8-1.2), and the total effective viable count is greater than or equal to 1 * 10 The complex microbial inoculant can effectively act on acidic sugarcane soil in places such as Guangxi, has multiple functions of dissolving phosphorus, dissolving potassium and secreting cellulase and protease, and can activate fixed nutrients in the soil, improve the utilization rate of a fertilizer, neutralize the acidity of the soil and improve the pH value of the soil. Pot experiments show that the bacterial agent can significantly promote sugarcane root development and plant growth, improve the fertilizer utilization rate, and provide an effective way for realizing high and stable yield of sugarcane and green improvement of acid soil.

The application discloses a kind of composite microbial inoculant and its application in improving the ability of crop to resist saline-alkali stress, the composite microbial inoculant includes the following weight fraction components: bacillus cereus 1-3 parts, bacillus megaterium 1-3 parts, hetero root sporocyst 6-8 parts, gypsum powder 50-70 parts, humic acid 10-30 parts, yeast metabolite 2-3 parts, aluminum sulfate 2-5 parts, citric acid 2-5 parts and adjuvant 0.4-0.6 parts.The application combines bacillus cereus, bacillus megaterium and hetero root sporocyst for the first time, and synergistically exerts its biological function, significantly improves the growth performance of crops under saline-alkali stress, by adding humic acid, yeast metabolite and other components, effectively improves the physical and chemical properties and nutrient status of saline-alkali soil, improves soil fertility, and provides a favorable environment for the growth of microbial inoculant;Significantly improve plant root activity, improve the salt tolerance of crops under saline-alkali stress conditions, improve the growth state of plants, thereby improving the yield of crops.

The invention discloses a growth-promoting bacterial fertilizer for pandan leaves and application of the growth-promoting bacterial fertilizer, and relates to the technical field of biology. Active components of the growth-promoting bacterial fertilizer for the pandan leaves comprise a microorganism combination, the microbial composition comprises bacillus altitudinis, bacillus megatherium, trichoderma longibrachiatum and aspergillus parasuis. Through the synergistic effect of the four strains, the growth-promoting bacterial fertilizer can effectively improve the soil environment of the saline-alkali soil, reduce the pH value of the soil and improve the enzyme activity of the soil and the nutrient effectiveness of the soil; meanwhile, growth and development of pandan leaves can be remarkably promoted, and plant height, stem diameter, fresh weight of single plant and acre yield are increased; the quality of pandan leaves can be improved, and the content of a characteristic aroma substance 2-acetyl-1-pyrroline is increased. The growth-promoting bacterial fertilizer is simple in preparation process, relatively low in cost, environment-friendly and particularly suitable for popularization and application in planting of pandan leaves in saline-alkali soil, and has important agricultural application value and market prospect.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.

The invention discloses a hypha three-dimensional carbon net composite carbon sequestration fungicide and a preparation method and application thereof. The preparation method comprises the following steps: firstly, placing aspergillus niger hyphae in a carbonization furnace for carbonization to obtain a hypha three-dimensional carbon net; and then putting the mycelium three-dimensional carbon net into a bacillus megatherium liquid, oscillating at constant temperature, filtering, and drying to obtain the mycelium three-dimensional carbon net composite carbon sequestration fungicide. When the compound is applied to improvement of soil fertility, the soil fertility can be comprehensively improved through multiple ways of regulating and controlling a soil carbon library, optimizing an acid-base environment, improving a physical structure, improving nutrient reserves and the like, and a basis is provided for application of the compound as a novel soil conditioner to agricultural production and promotion of soil health and sustainable agricultural development.

The invention relates to the technical field of microbial agents, in particular to a charcoal-based microbial agent and application thereof. The biochar-based microbial agent is prepared by mixing modified biochar and a composite microbial agent, the mass ratio of the modified charcoal to the complex microbial inoculant is (32-65): (11-24). The composite microbial agent comprises a bacillus velezensis microbial agent (Bacillus velezensis), a PBF-1 (Bacillus megaterium), a GPT-6 (Bacillus subtilis) and a bacillus subtilis microbial agent (Bacillus subtilis), and is characterized in that the composite microbial agent comprises a bacillus velezensis microbial agent (Bacillus velezensis), a PBF-1 (PBF-1), a GPT-6 (GPT-6) and a bacillus subtilis microbial agent (Bacillus subtilis). According to the biochar-based microbial agent, through deep fusion of materials and biotechnology, carrier-microorganism function complementation is achieved, and high efficiency, stability and sustainability are achieved.

The invention discloses a method for crystallizing BM3 protein in bacillus megaterium. The method comprises the following steps: constructing a gene for coding the BM3 protein into an expression vector, carrying out induced expression in escherichia coli, carrying out affinity chromatography purification, excising a fusion tag, carrying out gel filtration purification, incubating with cofactors FAD, FMN and NADPH to form a compound, and crystallizing under a specific pool liquid condition through a seated drop gas phase diffusion method. According to the method, the high-quality BM3 protein crystal suitable for X-ray diffraction analysis can be obtained, and a basis is provided for analyzing the three-dimensional structure of the BM3 protein crystal and researching an electron transfer mechanism.

The invention relates to the technical field of fertilizers, in particular to a functional fertilizer product for improving drought resistance of rice and an application method. The invention solves the problem of poor drought resistance of rice. The functional fertilizer product consists of basic nutrient elements, a microcapsule fungicide, an organic synergistic carrier, a composite synergist, a seaweed extract and a nano silicon fertilizer, the microcapsule fungicide is obtained by coating lysine bacillus sphaericus, bacillus megatherium and nitrogen-fixing bacteria with microcapsules; yeast paste and modified charcoal are introduced into the organic synergistic carrier; the modified biochar comprises chelating elements and biochar; the chelating elements comprise amino acid chelated zinc and glycine chelated manganese; the composite synergist adopts polyglutamic acid and potassium fulvate to cooperate with each other. The preparation method adopts a two-step method: firstly preparing the high-temperature-resistant inorganic material and the carrier into the basic master batch, and then adhering the heat-sensitive active substances such as the microcapsule fungicide to the basic master batch through a low-temperature coating technology. All the components have a synergistic effect, and the drought resistance of the rice can be remarkably enhanced.