193 results about "Bacillus megaterium" patented technology

The present invention provides a Bacillus megaterium strain comprising a purified population of microorganisms comprising one or more bacteria having a gyrB having at least 97% identity to SEQ ID NO: 1; and / or the population of microorganisms comprises one or more bacteria having a 16S rRNA having at least 97% identity to SEQ ID NO: 2. Optionally, the Bacillus megaterium strain has at least 97% identity to SEQ ID NO: 3. The strains may be used in compositions and methods.

The invention relates to the technical field of microbial deodorants, and discloses a microbial compound bacterium deodorant and a preparation method thereof.The microbial compound bacterium deodorant comprises a bacterium powder mixture, the bacterium powder mixture is embedded into a loading material, and the loading material is wrapped with a protective agent; the bacterial powder mixture comprises pediococcus acidilactici, bacillus megaterium, alcaligenes faecalis, bacillus subtilis and azotobacter chroococcum, wherein the mass ratio of the pediococcus acidilactici to the bacillus megaterium to the alcaligenes faecalis to the bacillus subtilis to the azotobacter chroococcum is 1: (1-3): (1-2): (0.5-1): (1-2). According to the microbial compound bacterium deodorant and the preparation method thereof, the prepared microbial compound bacterium deodorant can remarkably remove malodorous gases such as NH3 and H2S in biological excrement, the action time is long, the effect is remarkable, meanwhile, the raw materials of the microbial compound bacterium deodorant are green and safe, and the health problem caused by mistaken eating of livestock is solved.

According to the invention, the monooxygenase CYP102A1 from Bacillus megaterium is subjected to system modification and optimization, a series of monooxygenase mutants with improved activity and improved debenzylation selectivity are obtained, the activity of the mutant with the optimal effect is improved by 93.4 times compared with that of wild type monooxygenase CYP102A1, and the selectivity is improved to 99.5% from 33.2%. The monooxygenase mutant can be applied to catalytic synthesis of 3R, 5S-dihydroxy compounds, synthesis steps are simplified, reaction selectivity is improved, reaction conditions are mild, the monooxygenase mutant is environmentally friendly and efficient, and the overall efficiency of statin drug synthesis can be improved.

The invention discloses a magnetic carbon-loaded slow-release microbial agent which comprises an activated carbon carrier containing ferroferric oxide nanoparticles and a compound microbial bacterium composed of bacillus megatherium, bacillus mucilaginosus, white-rot fungi and pseudomonas, and the mass ratio of the compound microbial bacterium to the activated carbon carrier is (3-4): 30. The invention further discloses a preparation method and application of the magnetic carbon-loaded slow-release microbial agent. According to the magnetic carbon-loaded slow-release microbial agent disclosed by the invention, the activated carbon containing the ferroferric oxide nano-particles is used as a carrier to load the compound bacteria, so that the stability of the microbial bacteria is improved, the microbial bacteria can better resist the impact of the environment, the service life of the microbial bacteria is prolonged, and the slow-release effect of the microbial agent in the remediation of vegetable planting soil is achieved; the nutrient absorption of the vegetables is promoted.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The present invention relates to the technical field of microbial deodorants, and discloses a microbial composite deodorant and a preparation method thereof. The microbial composite deodorant comprises a bacterial powder mixture, which is embedded in a load material, and the load material is externally wrapped with a protective agent. The bacterial powder mixture comprises lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum, wherein the mass ratio of lactic acid Pediococcus, Bacillus megaterium, Alcaligenes faecalis, Bacillus subtilis, and Azotobacter chrysogenum is 1:1-3:1-2:0.5-1:1-2. The present invention adopts the above-mentioned microbial composite deodorant and a preparation method thereof. The prepared microbial composite deodorant can significantly remove malodorous gases such as NH3 and H2S in biological feces, has a long action time, and has a significant effect. At the same time, the raw materials of the microbial composite deodorant are green and safe, reducing health problems caused by accidental ingestion by livestock.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention relates to bacillus megatherium XW-02 (the preservation number is CCTCC NO: M 2025185), a microbial preparation of the bacillus megatherium XW-02, and application of the microbial preparation. The bacillus megatherium XW-02 has the advantages that the preservation number is CCTCC NO: M 2025185; the strain can efficiently express xylanase through xylan induction, hemicellulose in tobacco leaves is remarkably degraded, chemical composition is optimized, and combustion harmful substances are reduced. The treated cigar has the advantages that the aroma richness is improved, the purity is improved, the taste mellowness is improved, and the overall quality is improved by 1-2 grades. The xylan extracted from agricultural wastes is used as an inducer to construct a green biological manufacturing system, the cost is reduced by more than 80% compared with the traditional process, and the emission of three wastes is reduced. The process compatibility is high, the method can be matched with an existing production line only by additionally arranging a strain culture module, the single-line annual tobacco leaf treatment amount reaches 500 tons, and economic benefits are remarkable. The invention provides a safe, efficient and environment-friendly quality improvement solution for the tobacco industry, and has great industrialization value.

Provided are methods, prokaryotic host cells, expression vectors, and kits for the production of psilocybin derivatives produced using at least one psilocybin production gene. The methods enable production of psilocybin derivatives and related compounds including non-naturally occurring species. In certain embodiments, a prokaryotic host cell is selected from the group consisting of from the group consisting of Escherichia coli, Corynebacterium glutamicum, Vibrio natriegens, Bacillus subtilis, Bacillus megaterium, Escherichia coli Nissle 1917, Clostridium acetobutlyicum, Streptomyces coelicolor, Lactococcus lactis, Pseudomonas putida, Streptomyces clavuligerus, and Streptomyces venezuelae.

The invention discloses bacillus megaterium YK35 and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center on October 9, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32158; the strain has efficient potassium dissolving capacity and IAA (indoleacetic acid) producing and phosphorus dissolving capacity, and can remarkably promote the growth of crops; excellent stress resistance including heavy metal stress resistance, acid and alkali stress resistance, salt stress resistance and flooding stress resistance is achieved, and the adaptability is wide; the invention also provides a microbial agent containing the strain. Pot experiment and field experiment verification show that the microbial agent can promote the growth of plants; the yield and quality of the potatoes can be remarkably improved, and the effects of high yield and income increase are achieved; the application of potash fertilizer can be reduced through the potassium dissolving function of the fertilizer; the invention provides a new strain resource for the microbial fertilizer industry, and shows a strong application value in the aspect of growth promotion and yield increase of rhizosphere.

The invention discloses bacillus megaterium Sau.XJ-1 and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on October 21, 2024, and the preservation number of the strain is CCTCC NO: M 20242270. The strain Sau.XJ-1 obtained through screening can effectively convert insoluble potassium such as potassium feldspar and mica into soluble potassium capable of being absorbed by plants, and the utilization rate of the potassium element in soil is increased. After being prepared into a microbial agent, the microbial agent is applied to farmland soil, so that the content of soluble potassium in the soil can be remarkably increased, the growth and development of crops are further promoted, and the yield and the quality are improved. The method has important significance and application value in the aspects of cultivating and exerting soil ecological fertility and keeping agricultural ecological balance.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention belongs to the technical field of microbial fermentation and biological agriculture, and discloses a rice straw decomposition agent as well as a preparation method and application thereof. The rice straw decomposition agent is obtained by adopting bacillus megatherium (Bacillus megatherium) Z101 to be subjected to fermentation culture after activation of the bacillus megatherium Z101. The application method comprises the steps that harvested rice straw is flatly laid in a rice field, the water level of the rice field is kept to submerge the rice straw, then the rice straw decomposition agent is diluted with water and then sprayed to the surface of the laid rice straw for decomposition fermentation treatment, and decomposed rice straw is obtained. The rice straw decomposing inoculant is a fermentation culture solution of bacillus megatherium Z101, and compared with a fermentation culture solution obtained by conventional bacillus megatherium in the market, the rice straw decomposing inoculant has higher rice straw decomposing efficiency and speed, in-situ decomposing and field returning of rice straw are facilitated, and a new application way is provided for the bacillus megatherium Z101.

The invention relates to a method for repairing light rare earth and heavy metal compound polluted soil, which is characterized in that the compound polluted soil is repaired by adopting bacillus sp., the bacillus sp. Is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22085, and the preservation date is March 29, 2021. The repairing process comprises the following steps: mixing the bacillus subtilis bacterial liquid with the cementing liquid to obtain a mixed liquid; mixing the air-dried polluted soil with the mixed solution, and filling the mixture into a mold for reaction; the mold is communicated with a grouting pipe to inject the mixed liquid, and multi-round pumping is carried out; and after pumping is completed, the polluted soil is maintained and naturally air-dried. According to the method, dominant strains are adopted, biological adsorption and biological mineralization are combined, and effective control over the light rare earth and heavy metal compound contaminated soil is achieved under the action of dual bioremediation.

The invention relates to a biocontrol inoculant of bacillus megaterium Sneb753 for inducing rice to resist false smut and banded sclerotial blight and application of the biocontrol inoculant, and belongs to the technical field of microbial pesticides. The bacterial strain involved in the invention is bacillus megaterium Sneb753, which has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 16, 2024, and the preservation number is CGMCC No.33086. The invention also relates to a preparation method of the bacillus megaterium Sneb753. The bacillus megaterium Sneb753 can be prepared into a biocontrol inoculant which is used for inducing rice to generate rice false smut and rice sheath blight disease resistance through seed treatment or spraying, the rice false smut and the rice sheath blight disease of the rice can be effectively prevented and treated, and the growth of the rice can be promoted by treating the rice through fermentation liquor of the strain or an extracted metabolite; the bactericidal composition has a good application prospect in the aspects of preventing and treating rice false smut and banded sclerotial blight of rice.

The invention discloses application of bacillus megaterium capable of synthesizing indole-3-lactic acid to promotion of soybean growth and nodulation, the bacillus megaterium has the characteristic of promoting growth of various plants, and the fresh weight and nodulation of soybeans are remarkably improved; a large amount of indole-3-lactic acid (ILA) is synthesized and secreted, and the fresh weight and nodulation of soybean plants are remarkably improved; the strain has a good application prospect as a biological inoculant for leguminous crops.

The invention provides a complex microbial inoculant and application of the complex microbial inoculant in prevention and treatment of tobacco root rot, and belongs to the technical field of biocontrol inoculants. The biocontrol microbial inoculum disclosed by the invention is prepared by mixing and fermenting bacillus megatherium T482 and bacillus safensis YN-LJG-4. According to the invention, the two bacteria are subjected to co-fermentation, such that the composite bacterial agent is prepared. The bacillus megaterium T482 and the bacillus safensis YN-LJG-4 have a synergistic effect, so that the tobacco root rot can be effectively prevented and treated, and the tobacco quality can be improved. The complex microbial inoculant provided by the invention is simple in preparation method, has multiple effects, and shows a good application prospect in prevention and treatment of tobacco rhizome diseases as a biocontrol inoculant.

The invention discloses a microbial soil conditioner for saline-alkali soil. The microbial soil conditioner is prepared from the following raw materials in parts by mass: 5-20 parts of microbial flora, 60-85 parts of a carrier material, 5-15 parts of a functional additive and 3-8 parts of a water-retaining agent, wherein the microbial flora comprises halotolerant bacteria, phosphate solubilizing bacteria, nitrogen-fixing bacteria and potassium bacteria, and the volume ratio of the halotolerant bacteria to the phosphate solubilizing bacteria to the nitrogen-fixing bacteria to the potassium solubilizing bacteria is (2-4): (1-3): (1-2): (1-2). Wherein the halotolerant bacteria are bacillus subtilis, the phosphate solubilizing bacteria are bacillus megatherium, the nitrogen-fixing bacteria are brown nitrogen-fixing bacteria, and the potassium solubilizing bacteria are bacillus mucilaginosus. The invention relates to the technical field of agricultural soil improvement, in particular to a microbial soil conditioner for saline-alkali soil, which has the advantages of reducing the pH value and salt content of soil, improving the soil structure, promoting the growth of crops and protecting the environment due to the adoption of the technical scheme.

The invention belongs to the technical field of microbial fermentation and biological agriculture, and discloses a soil remediation fungicide as well as a preparation method and application thereof. The preparation method comprises the following steps: activating bacillus megatherium Z101, inoculating the activated bacillus megatherium Z101 into fermentation liquor containing rice straw powder, carrying out fermentation culture, and carrying out vacuum spray drying on fermentation mixed liquor after fermentation is completed, so as to obtain soil remediation bacterium powder; or further mixing the soil remediation bacterium powder with auxiliary materials and water, and granulating to obtain the soil remediation bacterium granules. The application method comprises the following steps: in a rice planting stage, applying the soil remediation fungicide and an inorganic fertilizer together to remedy rice field soil and promote rice growth. The soil remediation fungicide prepared by fermentation culture of the bacillus megatherium Z101 has remarkable advantages in the aspects of rice yield increase, soil pH value increase and physicochemical property improvement, and a new application way is provided for the bacillus megatherium Z101.

The invention discloses a microbial agent for promoting sand fixation in sand land and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises bacillus megatherium, pseudomonas fluorescens, lactobacillus mucus, trichoderma harzianum and azotobacter chroococcum which are mixed in the form of bacterial liquid, the volume ratio of the bacterial liquid is (3-5): (2-3): (2-3): (1-2): (1-2), and all bacterial strains have a synergistic effect. When the microbial agent is applied to soil, the soil stability is obviously improved after the microbial agent is applied, the water retention rate of sand land soil can be improved by 15.9 times within 10 days, the sand rising wind speed reaches 18.5 m / s and above, and the vegetation coverage can reach 32% and above after one year. The microbial agent disclosed by the invention is simple and convenient in preparation method, can remarkably promote sand fixation in the sand land and improve the survival rate of plants in the sand land, has the characteristics of environmental protection and high efficiency, and is easy to popularize and apply.

The invention belongs to the technical field of cortex acanthopanacis planting, and particularly relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method. The invention relates to an under-forest cortex acanthopanacis water and fertilizer integrated planting method, which comprises the following steps: S1, selecting a low-hill mountain land with the altitude of 200-400m and the gradient of lower than 15 degrees according to the tree age of 3-5 years and the canopy density of 0.25-0.35; s2, drip irrigation pipes are buried after soil preparation, then cortex acanthopanacis cutting slips are cut, clear water is poured thoroughly, and microbial water and fertilizer are dropped through the drip irrigation pipes in the subsequent planting process; wherein the microbial water fertilizer is formed by mixing bacillus safensis fermentation liquor and bacillus megatherium fermentation liquor. According to the method, in the under-forest cortex acanthopanacis integrated planting process, microbial water and fertilizer are subjected to drip irrigation, the prevention and treatment effect on cortex acanthopanacis root diseases can be effectively prevented and improved, and development of under-forest cortex acanthopanacis integrated planting is promoted.

The invention discloses a bacillus megaterium-containing microbial fertilizer for soil improvement and a preparation method thereof, and belongs to the technical field of fertilizers, the bacillus megaterium-containing microbial fertilizer for soil improvement is prepared from the following raw materials in parts by weight: 35-50 parts of an immobilized microbial agent, 3-5 parts of a water-retaining agent, 10-15 parts of humic acid and 2-5 parts of trehalose; the immobilized microbial agent is amino MOF (Metal Organic Framework) modified charcoal loaded bacillus megaterium; the microbial fertilizer is prepared from the immobilized microbial agent, the water-retaining agent, humic acid and trehalose, is high in pertinence and outstanding in performance in soil improvement, on one hand, available phosphorus in reclamation soil is activated, needed nutritional ingredients can be provided for plants, on the other hand, soil organic matter can be activated and updated, and the soil fertility is improved. The soil fertility is improved and the plant nutrient requirements are met.

The invention belongs to the technical field of microbial remediation, and particularly relates to bacillus megatherium 1-9-1, a microbial agent and application of the bacillus megatherium 1-9-1 and the microbial agent. The bacillus megaterium 1-9-1 is preserved in the China General Microbiological Culture Collection Center on December 21, 2023, and the preservation number of the bacillus megaterium 1-9-1 is CGMCC (China General Microbiological Culture Collection Center) No.29373. According to the application of the bacillus megatherium 1-9-1 in the aspects of repairing the overburden cracks and preventing the gas layer from escaping, the bacillus megatherium 1-9-1 is attached to the surface of the rock, carbonate precipitates are mineralized and deposited around the rock pores and the cracks for repairing, and meanwhile CO2 is absorbed and sealed. After the microbial agent provided by the invention is injected into a cover layer, the metabolite of the microbial agent can block pores and tiny cracks in the cover layer, so that the purposes of better carbon sequestration effect and oil and gas reservoir protection are achieved.

The invention relates to the technical field of biology, and particularly discloses bacillus megaterium LLH-B2 and application thereof. The invention relates to bacillus megaterium LLH-B2, which is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on November 14, 2022, and is named as bacillus megaterium LLH-B2, and the preservation number is CCTCC NO: M 20221787. The bacillus megaterium LLH-B2 is named as bacillus megaterium LLH-B2. The bacillus megaterium LLH-B2 can stably survive for a long time in a sandy soil drought environment, heavy metal treatment of sandy soil is efficiently treated, meanwhile, the soil fertility is improved, and plant growth is promoted.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

The invention relates to the technical field of microbial fertilizers. The invention aims to provide a fruit and vegetable biological source bacterial fertilizer system which comprises the following raw materials in parts by weight: 600 parts of wormcast, 200 parts of straw powder, 100 parts of humic acid, 1 part of a microbial agent and 5 parts of brown sugar. The microbial agent is prepared from bacillus subtilis, bacillus megatherium and actinomycetes according to the weight ratio of 5: 3: 2. According to the invention, the natural wormcast is used as a microbial carrier, and by virtue of the unique physical structure and microbial basis of the wormcast and in combination with a functional strain compounding technology, the defects of the traditional fertilizer can be effectively overcome.

Provided herein are screening methods and animal models relevant to ocular diseases such as age-related macular degeneration (AMD), e.g., for use in identifying candidate therapeutics for treating or preventing ocular diseases such as AMD. Also provided herein are compounds / compositions useful for killing or inhibiting the growth of microorganisms such as Bacillus megaterium. Further provided herein are methods of using the compounds / compositions to treat infections with microorganisms such as Bacillus megaterium, as well as to treat or prevent diseases or conditions associated with such infections, such as AMD.

The invention discloses a preparation method of a microbial complex microbial inoculant and a Chinese herbal medicine complex microbial inoculant and application of the microbial complex microbial inoculant in offshore fish culture, and belongs to the technical field of microbial engineering. The composite microbial inoculant comprises bacillus megatherium and rhodotorula mucilaginosa. In addition, the invention also provides a preparation method of the Chinese herbal medicine complex microbial inoculant, which comprises the following steps: mixing the microbial complex microbial inoculant, the Chinese herbal medicine and the base solution, and fermenting. In addition, the invention also provides a Chinese herbal medicine complex microbial inoculant prepared by the preparation method. In addition, the invention also provides a feed, and the mass addition amount of the compound microbial inoculant prepared by the preparation method in the feed is 5%-10%. In addition, the invention also provides an application of the Chinese herbal medicine complex microbial inoculant prepared by the preparation method in offshore fish culture. The microbial complex microbial inoculant can be used for Chinese herbal medicine fermentation, and the problem of obesity of fishes caused by artificial sweeteners can be effectively solved after the fishes are fed with the microbial complex microbial inoculant.

The application discloses a method for repairing light rare earth and heavy metal composite contaminated soil, and adopts bacillus megaterium (Bacillus megaterium) to repair the composite contaminated soil. Oceanobacillus sp. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center, has a preservation number of CGMCC No.22085, and is preserved on March 29, 2021. The repairing process is as follows: mixing bacillus megaterium liquid and cementing liquid to obtain a mixed liquid; mixing the air-dried contaminated soil and the mixed liquid, and then loading the mixed liquid into a mold for reaction; connecting a grouting pipe to the mold to inject the mixed liquid, and performing multiple rounds of pumping; after the pumping is completed, the contaminated soil is maintained and air-dried naturally. The application adopts the advantage strain, combines biological adsorption and biological mineralization, and realizes the effective control of the light rare earth and heavy metal composite contaminated soil under the action of double biological repair.