14 results about "Bacillus megaterium" patented technology

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The present application relates to the field of microbial technology, and particularly relates to a bacillus megaterium YBZ13 for preventing and treating walnut leaf scorching and application thereof. The bacillus megaterium YBZ13 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28098. The bacillus megaterium YBZ13 is a walnut rhizosphere symbiotic bacterium, and the bacterial suspension has a significant prevention and treatment effect on walnut seedling leaf scorching.

The application discloses a kind of microbial fertilizer and its in improving white pulp soil carbon sequestration capacity and application in promoting straw decomposition, belong to the technical field of microbial fertilizer.The microbial fertilizer includes organic matter, biochar, microbial inoculant, trace element and microbial activator;The microbial inoculant includes bacillus ND2 and bacillus megaterium CICC 22681;The microbial activator includes fulvic acid, gamma-polyglutamic acid and sorbitol.The microbial fertilizer of the application has the dual function of white pulp soil improvement and straw decomposition, can accelerate straw decomposition, improve white pulp soil structure, improve soil fertility and carbon sequestration capacity, help agricultural waste resourceization and soil quality improvement collaborative development, in white pulp soil area or other barren soil area in future agricultural production, it is expected to become important technical support for promoting sustainable development of ecological agriculture, and application prospect is wide.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The application discloses a rhizosphere phosphorus-fixing and phosphorus-dissolving microbial interaction regulation method and belongs to the field of microbial agents. 2+ and Mg 2+ bridge the two-way metabolite closed-loop mechanism of Bacillus megaterium exopolysaccharide, ferment by two-step method respectively and mix in an optimized ratio, prepare a synergistic microbial agent by compounding biochar and vermiculite into a heterogeneous carrier at a mass ratio of (2-4):(1-3), and apply the synergistic microbial agent in a circular ditch during the afforestation period of Litsea cubeba seedlings. The application breaks through the traditional cognition that functional antagonistic microbial groups cannot coexist, and the phosphorus accumulation amount of Litsea cubeba plants is increased by 28% compared with single phosphorus-dissolving microbial agent treatment.

The application relates to the field of self-repairing concrete, in particular to high-strength self-repairing concrete and a preparation method thereof. The high-strength self-repairing concrete comprises the following substances in parts by weight: cement 45-60 parts; recycled coarse aggregate 100-120 parts; fine aggregate 65-80 parts; water 10-20 parts; water reducing agent 0.1-0.3 parts; the recycled coarse aggregate is a recycled coarse aggregate with immobilized self-repairing microorganisms, and the self-repairing microorganisms comprise at least one of bacillus subtilis, bacillus megaterium and bacillus pasteurii. The application endows the concrete with crack self-repairing capability by immobilizing the self-repairing microorganisms. The microorganisms form dormant spores in the concrete, when cracks are generated and infiltrate water, the microorganisms are activated and metabolize calcium sources and urea in the surrounding environment, calcium carbonate crystals are generated through a microbial-induced carbonate precipitation mechanism, cracks are filled and structural integrity is restored. The process does not require external intervention and can significantly prolong the mechanical structure and mechanical properties of the concrete structure.

This invention discloses a bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers. By weight, the bio-fertilizer comprises the following raw materials: 30-40 parts of well-rotted livestock and poultry manure, 20-30 parts of straw or rice husks, 1-2 parts of borax, 2-3 parts of urea, 2-4 parts of ammonium sulfate, 2-3 parts of zinc sulfate, 2-3 parts of magnesium sulfate, 1-2 parts of humic acid, and 1-2 parts of a microbial compound agent. The microbial compound agent comprises 4-6 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-4 parts of Trichoderma echinococcus, 2-3 parts of Trichoderma viride, 3-5 parts of Pseudomonas fluorescens, 4-5 parts of Azotobacter chrysogenum, and 2-4 parts of Candida albicans. Using this bio-fertilizer and soil improvement method for reducing continuous cropping obstacles in cucumbers can significantly reduce the impact of continuous cropping obstacles, not only significantly reducing the incidence of cucumber wilt and root-knot nematodes and significantly promoting cucumber seedling growth, but also significantly increasing cucumber yield.

The invention discloses a gamma-PGA synthetase gene cluster pgsBCA '. The gamma-PGA synthetase gene cluster pgsBCA' is formed by sequentially connecting genes pgsB, pgsC and pgsA 'in series, the genes pgsB and pgsC are derived from a gamma-PGA synthetase gene cluster BA-pgsBCA of the bacillus altitudinis; the gene pgsA'is derived from a gamma-PGA synthetase gene cluster BM-pgsB 'C' A 'of bacillus megatherium. Bacillus amyloliquefaciens CF of which a genome knockout polyglutamate synthetase gene cluster is used as a host, and a recombinant polyglutamate synthetase gene cluster pgsBCA'is subjected to free expression through plasmids, so that a gene engineering strain capable of synthesizing gamma-L-PGA is obtained. When the strain is used for producing gamma-L-PGA in a 5L fermentation tank through amplification fermentation, the maximum yield can reach 10.5 g / L. Besides, the gamma-poly-L-glutamic acid is free of cytotoxicity and low in immunogenicity, so that the gamma-poly-L-glutamic acid has a wide application prospect in the fields of preparation of wound dressings, in-vivo absorption materials and the like. The invention provides a new material basis and technical support for application and development of polyglutamic acid in the fields of medicine and related biomedicine.

This invention relates to the field of biofertilizer technology, specifically disclosing a microalgae biofertilizer with soil remediation function and its preparation method. The preparation method includes: domesticating and culturing *Scenedesmus obliquus* liquid, *Cyanobacterium cyanobacteria* liquid, and *Monoflagellates* liquid with γ-aminobutyric acid-induced activation solution, and co-culturing them with *Bacillus polymyxa* and *Bacillus megaterium* to obtain a bioslurry; encapsulating the bioslurry with sodium alginate-carboxymethyl chitosan to obtain bioactive microspheres; impregnating corn straw with magnesium-iron salt precursors and pyrolyzing it under nitrogen to obtain modified biochar loaded with magnesium oxide and iron oxide, and compounding it with potassium humate to obtain a biochar suspension; and mixing the bioactive microspheres, biochar suspension, xanthan gum, zeolite, ammonium polyphosphate, potassium nitrate, zinc sulfate, potassium silicate, and deionized water evenly to obtain the microalgae biofertilizer with soil remediation function.

This application belongs to the field of mine ecological restoration and soil improvement technology, and particularly relates to compound microbial agents, soil conditioners, preparation methods, and applications. This application provides a compound microbial agent comprising: Bacillus subtilis, Trichoderma reesei, Trichoderma harzianum, Bacillus megaterium, and Bacillus mucilaginosus. This application also provides a soil conditioner comprising lignite and the compound microbial agent as raw materials, and its preparation method. This application further provides the application of the above-mentioned compound microbial agent or the above-mentioned soil conditioner or the above-mentioned soil conditioner preparation method in in-situ improvement of topsoil in black soil mining areas. In this application, the compound microbial agent decomposes the organic matter of lignite, promoting its conversion to humic acid, forming a synergistic effect with the indigenous microbial community of the soil. Utilizing the suitable environmental conditions of autumn soil temperature and humidity for microbial proliferation, the activation process is completed simultaneously with autumn land preparation, improving the efficiency of soil improvement. Tests have shown that applying the soil conditioner to the topsoil of the mining area to be improved can effectively improve soil conditions.

The application discloses a bacillus megaterium with the ability of producing aediron and high antioxidant capacity, and belongs to the field of microbial strains. The bacillus megaterium Y-3 is screened from black mud in a salt lake in Yuncheng, Shanxi, has high free radical scavenging capacity and strong iron ion reduction capacity, shows high antioxidant activity, and has the ability of producing aediron. The strain has great application potential in the fields of health products and cosmetics.

The application belongs to the technical field of planting and fertilization, and particularly relates to a diversity planting method for improving phosphorus fertilizer utilization efficiency of sunflower in saline-alkali soil, which solves the technical problem of low phosphorus fertilizer utilization efficiency of the existing sunflower planting method. The method first uses desulfurized gypsum as a soil conditioner, plants sunflower by ridge planting, and plants wild soybean, sueda and alfalfa in diversity, realizes inter-ridge crop coverage to reduce evaporation and suppress salt, cooperates with a composite phosphorus-solubilizing microbial agent containing bacillus subtilis, bacillus megaterium and pseudomonas fluorescens, enhances soil phosphorus-solubilizing microbial activity, and through precise water and fertilizer integrated management during the growth period of the sunflower, according to the needs of different growth periods, urea, potassium sulfate, potassium dihydrogen phosphate and other fertilizers are driped to ensure sufficient and balanced nutrition supply. The diversity planting method significantly improves the phosphorus fertilizer utilization efficiency in saline-alkali soil, is suitable for practical application in saline-alkali soil, and has wide popularization value.

This invention belongs to the field of biomass utilization and organic water-soluble fertilizer technology, specifically relating to an organic water-soluble fertilizer for improving acidic soil and its preparation method. The preparation method includes the following steps: rice straw is crushed and mixed with beet pulp and soybean meal, then sterilized at high temperature. It is then inoculated with Bacillus megaterium Z101 activated bacterial solution for solid-state fermentation. The resulting fermentation material is mixed with KOH solution and heated to 120-180℃ for hydrothermal treatment. The filtrate is filtered to obtain a potassium humate solution. The potassium humate solution is then mixed and dissolved with an organosilicon synergist and water-soluble silicon fertilizer to obtain the organic water-soluble fertilizer for improving acidic soil. The organic water-soluble fertilizer of this invention uses a combination of biochemical potassium humate obtained from solid-state fermentation of rice straw with Bacillus megaterium Z101 and water-soluble silicon fertilizer, further combined with an organosilicon synergist, which can synergistically improve the physical properties of acidic soil and increase crop yield.