Compound microbial bacterial preparation for efficiently degrading feces and preparation method and application of compound microbial bacterial preparation

A technology for compounding microbial inoculants and feces, applied in the field of microbial fermentation, can solve problems such as unsatisfactory processing speed and effect, and achieve the effect of shortening fermentation time

Inactive Publication Date: 2012-09-26
DEZHOU YUANHE AGRI TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composite bacterial agent can enhance the activity of the total microbial flora and lignocellulose-degrading bacteria in the pile, and can treat sewage, but it is not ideal for the treatment speed and effect of poultry manure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A preparation method for a compound microbial bacterial agent for efficiently degrading feces, comprising the steps of:

[0050] (1) Bacillus subtilis CICC10088 was inoculated in LB liquid medium, cultured at 30°C for 24 hours, then inoculated into solid medium A at an inoculum amount of 10wt%, and cultured at 37°C for 3 days to obtain subtilis containing Bacillus CICC10088 viable count 10×10 9 Each / g of solid microbial agent A;

[0051] (2) Inoculate Bacillus cereus CICC20515 in LB liquid medium, culture at 30°C for 24 hours, then inoculate Bacillus cereus in solid medium B at an inoculum amount of 10 wt%, and culture at 30°C for 3 days, the number of live bacteria containing Bacillus cereus CICC20515 was 8×10 9 A / g of solid bacterial agent B;

[0052] (3) Bacillus megaterium CICC120611 was inoculated in LB liquid medium, and cultured at 30°C for 24 hours, then inoculated Bacillus megaterium in solid medium C at an inoculation amount of 10 wt%, and cultured at 30°C ...

Embodiment 2

[0069] Adopt the method as described in Example 1, the difference is that step (9) solid bacterial agent A, solid bacterial agent B, solid bacterial agent C, solid bacterial agent D, solid bacterial agent E, solid bacterial agent F, solid bacterial agent The mixing weight ratio of bacterial agent G and solid bacterial agent H is 3:1:3:1:3:1:3:1.

[0070] After testing, in the prepared composite microbial agent for degrading feces, each gram of the compound microbial agent contains: the number of live bacteria of Bacillus subtilis (Bacillus subtilis) 1.87×10 9 pcs, Bacillus cereus (Bacillus cereus) viable count 5×10 8 , Bacillus megaterium (Bacillus megaterium) live bacteria count 3.57×10 8 1. The number of live bacteria of Geobacillus stearothermophilus 1.25×10 8 , Asperqillus oryzae spore number 2.81×10 8 The number of spores of Trichoderma viride 9.37×10 7 1. The number of spores of Streptomyces microflavus 1.87×10 8 1, the number of spores of Sporotrichum thermophilic ...

Embodiment 3

[0072] Adopt the method as described in Example 1, the difference is that step (9) solid bacterial agent A, solid bacterial agent B, solid bacterial agent C, solid bacterial agent D, solid bacterial agent E, solid bacterial agent F, solid bacterial agent The mixing weight ratio of agent G and solid bacterial agent H is 2:1:1:1:2:1:1:1.

[0073] After testing, in the prepared compound microbial agent for degrading feces, each gram of compound microbial agent contains: Bacillus subtilis (Bacillus subtilis) viable bacteria count 2×10 9 , Bacillus cereus (Bacillus cereus) live bacteria count 8×10 8 , Bacillus megaterium (Bacillus megaterium) live bacteria count 2×10 8 1, the number of live bacteria of Geobacillus stearothermophilus (Geobacillus stearothermophilus) 2×10 8 , Asperqillus oryzae spore number 3×10 8 1. The number of spores of Trichoderma viride 1.5×10 8 10 spores of Streptomyces microflavus 8 1, the number of spores of Sporotrichum thermophilic 10 8 indivual.

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PUM

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Abstract

The invention relates to a compound microbial bacterial preparation for efficiently degrading feces and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, bacillus megaterium, geobacillus stearothermophilus, aspergillus oryzae, trichoderma longibrachiatum, sporotrichum thermophile and streptomycesmicroflavus through solid fermentation. The compound microbial bacterial preparation can shorten the fermentation time of feces, and effectively kill larva, eggs and pathogens in the feces, and further improves the content of organic matters, total nitrogen, total phosphorus, and total potassium in the fermented materials.

Description

technical field [0001] The invention relates to a compound microbial bacterial agent for efficiently degrading feces, a preparation method and application thereof, and belongs to the technical field of microbial fermentation. technical background [0002] With the improvement of the intensification of the breeding industry and the continuous expansion of the production scale, the rapid increase in the number of livestock and poultry has led to an increase in the discharge of manure. A large amount of livestock and poultry manure is discharged into the environment without treatment, which not only pollutes the environment, And it will also lead to the spread of pathogenic bacteria, seriously endangering the health of livestock, poultry and human beings. [0003] Livestock and poultry manure is rich in crude protein, crude fiber, crude fat, minerals, nitrogen, phosphorus, potassium and other nutrients, and is a cheap raw material for producing high-quality organic fertilizers....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F11/08C12R1/125C12R1/085C12R1/11C12R1/465C12R1/01C12R1/69C12R1/885C12R1/645
CPCY02W30/40
Inventor 展宁杨瑞
Owner DEZHOU YUANHE AGRI TECH DEVCO
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