185 results about "Thermophile" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention relates to a compound microbial bacterial preparation for degrading crop straw and a preparation method and application of the compound microbial bacterial preparation. The compound microbial bacterial preparation is prepared from bacillus subtilis, bacillus cereus, aspergillus niger, aspergillus flavus, trichoderma reesei, trichoderma longibrachiatum, sporotrichum thermophile and phanerochaete chrysosporium through solid fermentation. The compound microbial bacterial preparation can shorten the decomposition time and improve the decomposition efficiency of the crop straw. Microorganisms generate various effective metabolic products with nitrogen fixation and phosphate solubilizing functions and a high disease and pest resistance effect in the fermentation process, so that the compound microbial bacterial preparation is favorable for improving the soil structure and the yield and the quality of crops.

A high-temperature aerobic fermentation of organic waste and its apparatus are disclosed. The process is carried out by using thermophile, breaking biodegradable substances in organic waste as raw materials, entering broken materials into primary fermenter, lifting temperature and inoculating, supplying aeration, entering materials into secondary fermenter, supplying aeration, adding ferment additive, intensified fermenting, entering materials into grate to separate varia, solid-liquid separating, forming filter cake, processing filter cake into solid fertilizer, and processing filtrate into liquid fertilizer. It has short time and can be used for food refuse and urban mud.

The invention discloses a multifunctional agricultural microbial agent as well as an application method and effects thereof. The microbial agent is compounded from 1-3 parts by weight of bacillus subtilis, 0.5-2 parts by weight of Bacillus licheniformis,1-2 parts by weight of Sporotrichum thermophile, 0.5-1 part by weight of aspergillus oryzae and 0.5-3 parts by weight of trichoderma viride, and has the effective viable count of 5*10<8> per g. The microbial agent has a plurality of functions, is capable of decomposing organic materials and deodorizing, and can be applied to preventing diseases and increasing production in crop planting. The organic materials refer to organic waste materials rich in cellulose and protein such as excrements of livestock, crop straws, sludge, compostable household refuse and spent mushroom compost. The microbial agent is applied to treating the organic materials, and has better effects than the control without the microbial agent in the aspects of microbial content, available nutrient, C/N ratio, odor and fermentation period; the period of fermentation by using the microbial agent is 15 days, while the period of fermentation of the control is 32 days; and if the microbial agent is applied to cucumber planting, the disease control efficiency can be 60.20% and the production increase per mu can be 15.6%.

The invention provides a heavy metal sludge soil restorer, a preparation method and an application thereof in sludge elimination. The adopted technical scheme comprises: municipal sludge is subjected to pretreatment, thermophiles are used for decomposing organic substances, and a heavy metal is added to treat microorganisms. After the sludge restorer is used in a test, the effective removal rate of various pollutants in soil reaches 95% or above, in line with national standards. At the same time, sulfate can be used to repair heavy metal land in situ, and is also a very good organic fertilizer, and is beneficial to reduction in the use of chemical reagents and chemical fertilizers in agricultural production, thereby eliminating pollution sources and secondary pollution from the source.

The invention discloses a tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof. Plasmid T-GFP is constructed by using plasmid rDNA-GFP in the methods such as enzyme cutting and PCR enzyme cutting site mutation; thermophile tetrahymena genome DNA is used for constructing plasmid T-HSP702 in the methods such as PCR augmentation and clone; Plasmids T-GFP, T-HSP702 and pD5H8 are used for construction and finally obtaining plasmid HSP702-GFP-pD5H8 by enzyme cutting and connection. The carrier contains HSP702 promoter, thereby being capable of realizing high-efficient expression of exogenous gene; the carrier contains replaceable exogenous gene gfp so that an exogenous gene expression system is realized for tetrahymena by replacing different exogenous genes; with the sieving effect of paromomycin medicine, the carrier replaces most rDNAs originally in the tetrahymena so that the exogenous genes are largely increased in the tetrahymena so as to realize genetic transformation of target genes. The method in the invention is easy and has convenient operation, and the carrier can be used for detection of environmental tributyl tin in transfection in tetrahymena.

The invention provides a method for producing hydrogen and/or methane through fermentation of fiber wastes and a device thereof. The method comprises the following steps: smashing a straw into a certain grain diameter, placing into a hydrogen production reactor, adding a given amount of water and nutrient solution and then mixing with thermophile bacteria seed liquid together for fermentation to prepare the hydrogen; pumping hydrogen fermentation liquid into a liquid storage tank, continuously pumping into the hydrogen production reactor for fermentation to prepare the methane after pH is adjusted. Because of adopting fiber wastes, raw materials used by the invention have rich resource and low cost, effectively solve the resource problem of biological hydrogen production and overcome the problem that the fiber raw materials of the traditional straw are hard to effectively produce the hydrogen when being not subjected to pretreatment; and the semi-continuous hydrogen production is achieved through the fiber waste dynamic immobilization technology. The invention is easier to popularize and apply by adopting a coupled continuous methane production system and has the advantages of simple and highly-efficient method, energy conservation, low cost and the like.

The present invention discloses a production method of solid yogurt powder containing high active bacteria. Firstly, a yogurt fermentation agent is prepared by a high density culture, lactobacillus bulgaricus and streptococcus thermophiles are respectively activated and mixed, the mixed bacteria are inoculated into a sterile lactic acid solution to obtain a high density bacterial liquid, a cryoprotectant is added, and the bacterial liquid is freeze-dried to obtain the yogurt fermentation agent; secondly, the a yogurt containing the high active bacteria is obtained by a secondary fermentation, and the condition is as follows: milk powder is mixed with water, a first yogurt fermentation agent is inoculated, the fermentation is conducted for 4-6 h, and a second fermentation agent is inoculated, the fermentation is conducted for 2-4 h; and finally, lactose, whey protein and skim milk powder are mixed with the yogurt, the mixture is homogenized, and the homogenized mixture is subjected to a low temperature spray drying to obtain the yogurt powder. The produced yogurt powder is high in the number of viable bacteria. Due to the fact that the number of the viable bacteria is high in the yogurt powder, the blending time is greatly shortened.

The invention discloses a method for producing acid soybean peptide protein by using an enzyme method together with microorganisms. The method consists of the following steps of: (1) smashing and sieving commercially available peeled soybean meal, adding glucose and purified water, inoculating lactobacillus plantarum and streptococcus thermophiles, and culturing till the pH of a zymotic fluid is lowered to 4.4 to obtain a zymotic fluid A; (2) inoculating bifidobacteria into the zymotic fluid A and adding liquid acid protease for performing enzymolysis to obtain an enzymolysis solution A; and (3) sterilizing ultrafiltration permeate liquid obtained by performing ultrafiltration on the enzymolysis solution A, concentrating and drying to obtain a finished product, wherein the lactic acid content of the finished product is higher than 3.6 percent. In the method, peeled soybean meal which is much cheaper than the conventional generally-adopted soybean protein concentrate is taken as a raw material. Technically, enzymolysis is performed after lactobacillus plantarum and streptococcus thermophiles are used of lowering the pH of the entire enzymolysis system to pH which is most suitable for the acid protease; and meanwhile, a part of soluble non-starch polysaccharides in the peeled soybean meal can be degraded in the growing processes of the lactobacillus plantarum and the streptococcus thermophiles, and the finished product also contains organic acid substances.

A system for the efficient conversion of plant biomass to methane is provided, where the conversion includes use of a thermophilic methanogenic consortium containing a cellulolytic thermophile, an acetate-oxidizing thermophile and a thermophilic methanogen, the combination of which hydrolyzes hexoses and pentoses, oxidizes acetate and provides a hydrogen sink, to convert plant biomass to the theoretical limit of bioenergy.

The invention discloses a compound microorganism fertilizer specially used for corns and capable of improving soil and a preparation method thereof. The preparation method comprises the steps of: firstly deodorizing fresh poultry dung and cow dung, then adding sporotrichum thermophile, aspergillus niger, distillers' grains, brown sugar, ground straws, corn meal and potassium dihydrogen phosphate to carry out fermentation twice and then drying the product to obtain a preparation I; then taking soybean meal, wheat bran, calcium meal, bacillus megaterium, bacillus subtilis, lactobacillus, cane sugar and ferrous sulfate to carry out fermentation twice and drying the product to obtain a preparation II; and then mixing the preparation I with the preparation II, then adding bacillus megaterium, bacillus subtilis, aspergillus niger, streptomyces alboniger, terrestrial cyanobacterium, photosynthetic bacteria and dinoflagellate to carry out fermentation and finally adding chemical fertilizers needed by corns, thus obtaining the compound microorganism fertilizer specially used for corns. The prepared product contains surviving beneficial microorganisms and can improve the crumb structure and other indexes of soil and increase the corn yield.

In recent years, with the rapid growth of the traditional edible fungus industry all over China, the pollution and the hazard generated to the entironment and the edible fungus industry per se by a large number of obsolete fungus sticks (slags) after the primary planting of edible fungi also become bigger and bigger. The invention provides a method for rapidly fermenting and treating obsolete fungus sticks of edible fungi by utilizing sporotrichum thermophile, thereby not only realizing the comprehensive development and the deep utilization of wastes, but also reducing pollution sources to a large degree. In the invention, sporotrichum thermophile is utilized to rapidly ferment the obsolete fungus sticks and the fungus slags of the edible fungi, wherein the fermentation temperature is controlled within the range of 45-70 DEG C; fermentation lasts for 10-15 days, and compared with the traditional fermentation, the fermentation days are shortened by 8-10 days; the fermentation is complete, and harmful pests and eggs can be removed thoroughly; a variety of dedicated organic fertilizers can be prepared if the decomposition products of the fungus sticks are inoculated into other functional strains, coordination factors and the like; the weight proportion of the decomposition products is higher than 50 percent, and the decomposition products have high and stable activity, being beneficial to roots to absorb nutritive materials; the decomposition products can also serve as the compost for the secondary planting of the edible fungi and have convenient operation; common peasant households can prepare and then use the decomposition products on the spot and have low costs and are convenient and quick, thereby being easy to popularize.

The invention discloses a preparation method and application of dendrobium officinale yoghurt. The preparation method comprises uniformly mixing whole milk powder, water and cane sugar, or fresh milk and cane sugar, and performing homogenization processing; then adding dendrobium officinale fine powder, performing sterilization processing, then adding mixed bacteria composed of lactobacillus bulgaricus and streptococcus thermophiles, stirring uniformly, adding into a container, performing sealing, fermenting, cooling and post-ripening, so as to obtain the dendrobium officinale yoghurt which possesses high probiotics living bacterium quantity and contains dendrobium officinale active composition. The preparation method possesses advantages of short fermentation period, simple operation and low cost. The prepared dendrobium officinale yoghurt is applicable to prepare food possessing functions of treating spleen deficiency and constipation and treating antibiotic-related diarrhea.

The invention provides a lactic acid bacteria powder for suckling pigs as well as a preparation method and a using method thereof and belongs to the technical field of microorganism feed additives. The adopted lactic acid bacteria comprise one or more of enterococcus faecalis, enterococcus faecium, pediococcus acidilactici, streptococcus thermophiles, lactobacillus acidophilus, lactobacillus plantarum and lactobacillus casei. The active viable count is 50-1000 hundred million/gram and the water content is 4-8%. When the lactic acid bacteria are ingested by newborn suckling pigs through the way spraying the lactic acid bacteria powder obtained by using the preparation method in mouth cavities of the newborn suckling pigs or on nipples of sows, the death rate of the suckling pigs is reduced; the rate of sick pigs with red or white dysentery is reduced; the weight is increased; the suckling pigs are vivacious and bright in fur; the special effects of the lactic acid bacteria are fully developed.

The invention discloses a method for preparing a special composite microbial fertilizer for tobacco and capable of improving soil, and a prepared product. The preparation method comprises the following steps of: deodorizing fresh chicken manure, adding sporotrichum thermophile, lactic acid bacteria, thermoactinomyces, brown sugar, crushed straws, flour, corn flour and monopotassium phosphate, fermenting for twice and performing air drying to obtain a preparation I; fermenting bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate for twice, and performing air drying to obtain a preparation II; and mixing the preparation I and the preparation II, adding bacillus megatherium, bacillus subtilis, aspergillus niger, streptomycete, terricolous cyanobacteria, photosynthetic bacteria and dinoflagellate, fermenting, and adding chemical fertilizers needed for growth of the tobacco to obtain the special composite microbial fertilizer for the tobacco. The prepared product contains viable beneficial microorganisms and can be used for improving soil aggregated structure and other indexes and increasing the yield of tobacco leaves.

The invention relates to the field of food production and processing, and particularly relates to high-protein yogurt. The yogurt is prepared from the following components: 0.5-6 percent of white granulated sugar, 0.02-0.5 percent of lactobacillus bulgaricus, 0.01-0.6 percent of streptococcus thermophiles and the balance of milk by means of fermenting and serum removing. Currently, varieties of high-protein yogurt in China are increased year by year, but the content of protein in the yogurt is generally low, and the yogurt products have the defects of serious powder taste and sticky texture. The high-protein yogurt is prepared by combining a serum separation technology and homogenizing process, the content of natural milk protein in the yogurt can be 8-10 percent, which is increased by 40percent or more than that of existing high-protein yogurt, no protein powder or other stabilizer is required, and the product has natural flavor, smooth texture and refreshing taste. Based on the sameconcept, the invention further discloses high-protein viable type yogurt which can be stored at normal temperature and drinking type high-protein yogurt.

The invention relates to a method for processing sludge by utilizing a thermophile bacteria reinforcement ATAD (Autothermal Thermophilic Aerobic Digestion) process. The method comprises the following steps of: (1) preparing an advantage bacterial agent; (2) carrying out pre-concentration processing; (3) carrying out ATAD processing; and (4) carrying out storage. According to the invention, thermophile bacteria with strong capacity of degrading organic matter in the sludge are screened and acclimatized by adopting a modern biotechnology; the method does not just depend on the thermophile bacteria of the sludge; the short-time high-efficiency degradation characteristic for the sludge is realized by utilizing an active extracellular enzyme secreted by the thermophile bacteria at high temperature; the method is combined with the ATAD process to improve reduction efficiency and harmless degree of the sludge; a novel technological approach is provided for fulfilling the aims of reduction, harmlessness and reutilization of the sludge; wider technical means and process are developed; and cost of processing and disposing the sludge is reduced.

The invention discloses a method for preparing a special composite microbial fertilizer for non-deciduous fruit trees and capable of improving soil, and a prepared product. The preparation method comprises the following steps of: deodorizing fresh chicken manure and dairy cow manure; adding sporotrichum thermophile, aspergillus niger, brown sugar, crushed straws, vinasse and monopotassium phosphate, fermenting and performing air drying to obtain a preparation I; fermenting bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate, and performing air drying to obtain a preparation II; and mixing the preparation I and the preparation II, adding bacillus megatherium, bacillus subtilis, aspergillus niger, streptomycete, terricolous cyanobacteria, photosynthetic bacteria and dinoflagellate, fermenting, and adding chemical fertilizers needed for growth of the non-deciduous fruit trees to obtain the special composite microbial fertilizer for the non-deciduous fruit trees. The prepared product contains viable beneficial microorganisms, and can be used for improving soil aggregated structure and other indexes, and increasing the yield of fruits.

The invention discloses a compound remediation agent for clearing soil contaminated by petroleum and a remediation method of the compound remediation agent. The compound remediation agent is preparedfrom the following components in parts by weight: 10-20 parts of biochar, 3-5 parts of monopotassium phosphate, 4-6 parts of calcium nitrate, 3-6 parts of perlite, 3-8 parts of ceramsite, 2-5 parts ofwood chips, 5-10 parts of vermiculite, 2-6 parts of magnesium sulfate, 3-6 parts of pine needle, 5-8 parts of chicken manure and 15-30 parts of microbial preparation, wherein the microbial preparation is prepared from the following bacterium suspension in parts by weight: 10-15 parts of bacillus thermophiles, 5-8 parts of bacillus natto, 6-10 parts of aspergillus niger, 2-6 parts of bacillus subtilis, 2-5 parts of sulfur bacteria, 3-7 parts of photosynthetic bacteria and 2-5 parts of saccharomycetes; the bacterium suspension is obtained by performing fermentation and culturing on various microorganisms and has the concentration being larger than or equal to 10<5>cfu/mL. The invention discloses a remediation device for performing soil remediation by using the compound remediation agent forclearing the soil contaminated by the petroleum. Through the method for remediating the soil with the compound remediation agent provided by the invention and the remediation device, the compound remediation agent can be fully contacted to the contaminated soil, so that the remediation effect is remarkably improved to 96.5 percent, and secondary pollution to the soil cannot be caused.

The invention discloses a preparation method and application of a composite bacteria preparation for a livestock and poultry fermented feed. Selected lactic acid bacteria are anaerobes which are subjected to homolactic fermentation, mainly comprise enterococcus faecium, enterococcus faecalis and streptococcus thermophiles, are the most common lactic acid bacteria in the livestock and poultry industry; no gas is substantially produced in a metabolism process; a fermentation system is only required to keep better tightness; and the composite bacteria preparation has better applicability for fermentation of most of livestock and poultry feeds. Selected yeast is bread yeast, i.e., the yeast which is circulated on the commercial market for producing bread; and every gram of bread yeast dry powder contains 100 hundred millions of cfu active yeasts; the water content of the prepared composite bacteria preparation generally does not exceed 9.0 percent; the composite bacteria preparation can be stored for 3-6 months under a vacuum package condition; the number of the yeasts is kept basically unchanged; and the number of the active lactic acid bacteria is kept at about 50-65 percent. The fermentation can be excellently started by inoculating 0.1-0.2 percent of composite bacteria product in the livestock and poultry fermented feed.

The invention relates to a method for preparing a bile salt hydrolase lactobacillus powder starter, which is suitable for producing powder starters for fermented dairy products such as functional yoghourt and probiotics preparations. The method comprises the following steps of: performing activation and amplification culture on Lactobacillus casei KL1, Lactococcus lactis lactis KS4 and Streptococcus thermophiles Tx, which are screened from Tibetan kefir and are strains of bile salt hydrolase; performing high density culture by using an automatic fermentation tank; performing centrifugal concentration and adding a freeze-drying protective agent for prefreezing and freeze drying to prepare the bile salt hydrolase lactobacillus powder starter. After the powder starter prepared by the method is used for fermenting milk, the obtained yoghourt has sticky, fine and smooth mouthfeel, moderate sweet and sour taste, and thick ester aroma.

The invention belongs to the technical field of renewable energy sources and particularly relates to a method for producing hydrogen by fermenting an organism and alkali composite pretreated straw fiber raw material at two steps. The method comprises the following steps of: (1) crushing the straw fiber raw material and mixing the crushed raw material with a nutritive salt solution to obtain a mixture; (2) introducing a thermophile bacteria seed solution into the mixture and carrying out the first-step anaerobic fermentation to produce hydrogen gas; (3) mixing fermentation residue, water and sodium hydroxide according to the proportion for alkali pretreatment, pre-treating the residue and washing to be neutral to obtain a compound pretreated straw fiber; and (4) mixing the obtained compound pretreated straw fiber with the nutritive salt solution, introducing the thermophile bacteria seed solution into the mixture and carrying out the second-step anaerobic fermentation to produce hydrogen gas. Compared with the traditional method for producing the hydrogen by pre-treating the straw with alkali, the method for producing hydrogen by fermenting the composite pretreated straw fiber at two steps ensures that the hydrogen-producing level is improved by 75 percent, the utilization rate of the raw material is improved to 70 percent from 40 percent and the expense for the alkali pretreatment is reduced by 40 percent.

The invention discloses a preparation method of a liquid yoghurt starter. According to the preparation method, streptococcus thermophiles at a stationary phase are inoculated in an RY culture medium in the proportion of 4%-6%, subjected to static culture at the temperature of 42 DEG C for 4-6 hours and then inoculated with lactobacillus delbrueckii subsp. buglaricus at a stationary phase in the proportion of 2% for continuous culture for 10-12 hours, and then the liquid yoghurt starter is prepared. The preparation method of the liquid yoghurt starter is simple in technology, bacterium concentration and freeze drying are not required to be performed on the liquid yoghurt starter, the bacterium vitality is high, the strain proportion is proper, and the method can be directly applied to yogurt fermentation and is suitable for small, medium and large dairy processing enterprises.

A disclosed organic biological fertilizer for pomegranate trees is prepared from the following raw materials in parts by weight: 20-40 parts of rice bran, 10-15 parts of vinasse, 15-25 parts of peat, 15-25 parts of soybean cake powder, 15-20 parts of turf, 15-20 parts of corn straw, 10-20 parts of chicken manure, 10-15 parts of wheat bran, 5-7 parts of ferrous sulphate, 3-6 parts of ammonium sulfate, 6-8 parts of magnesium sulfate, 3-9 parts of borax, 4-7 parts of potassium fulvate, 2-6 parts of marine spirillum powder, 1-4 parts of bacillus megaterium, 2-5 parts of sporotrichum thermophile, 3-6 parts of microzyme, 4-7 parts of cellulase and 5-8 parts of lactic acid bacteria powder. The provided organic biological fertilizer for pomegranate trees is capable of improving output and sugar content of pomegranate without pollution.

The invention relates to the field of fermentation engineering and discloses a hericium erinaceus, fruit and vegetable drink and a production method thereof. The production method comprises steps as follows: a), a hericium erinaceus fruiting body is smashed, purified water is added, the mixture is extracted with hot water, and then an extract liquid is concentrated; b), multiple fruits and vegetables are washed, sterilized with ultraviolet, cut into slices and juiced, and white granulated sugar is added and mixed; c), streptococcus thermophilus, lactobacillus bulgaricus and lactobacillus plantarum are transferred to an MRS culture medium from corresponding slopes respectively, and enlarging cultivation is performed; d), fruit and vegetable juice and hericium erinaceus concentrates are mixed in proportion, and hericium erinaceus, fruit and vegetable juice is obtained; e), streptococcus thermophiles, lactobacillus bulgaricus and lactobacillus plantarum are added to the hericium erinaceus, fruit and vegetable juice in different proportions, and the mixture is left to stand for fermentation. The quality of the hericium erinaceus, fruit and vegetable drink is stable, the drink has a moderate sweet and sour taste, the SOD (superoxide dismutase) activity is high, the product stability is good, and the hericium erinaceus, fruit and vegetable drink has the functions of delaying ageing and boosting immunity.