Multifunctional agricultural microbial agent as well as application method and effects thereof

A microbial inoculant and multi-functional technology, which is applied to the application field of agricultural microorganisms in crop cultivation and organic waste treatment, can solve the problems affecting the enthusiasm of enterprises and farmers, high production cost, low added value, etc., to improve crops. quality, reduce the use of chemical fertilizers, and promote the effect of increasing income

Active Publication Date: 2014-08-13
天蓝地沃(天津)农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the functions of various agricultural microbial agents are mainly single. For example, some are used as decomposing agents for organic materials, some are used as bacterial fertilizers, and some are used as soil remediation agents for disease resistance. The functions are single, the production cost is high, and the added value is low. Low, less agricultural income, affecting the production of enterprises and the enthusiasm of farmers to use, thus affecting the rapid resolution of the above problems

Method used

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  • Multifunctional agricultural microbial agent as well as application method and effects thereof
  • Multifunctional agricultural microbial agent as well as application method and effects thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of media and strains

[0033] 1. Preparation of culture medium

[0034] 1. Potato and dextrose agar medium (PDA)

[0035] Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.

[0036] 2. Nutrient gravy agar

[0037] It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).

[0038] 3. Fungal solid fermentation medium

[0039] 80g wheat bran, 10g soybean meal, (NH 4 ) 2 SO 4 1.0g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 00.05g, 80mL distilled water.

[0040] 2. Activation of bacteria

[0041] Bacillus subtilis and Bacillus licheniformis were inoculated on nutrient bou...

Embodiment 2

[0053] Mutual antagonism experiment between strains

[0054] The activated Bacillus subtilis, Bacillus licheniformis, Sporosporium thermophila, Aspergillus oryzae and Trichoderma viride were subjected to mutual antagonism experiments between strains, and they were combined in pairs, divided into 20 combinations in total, and each combination was repeated three times.

[0055] The steel ring method was used for the antibacterial test, and the strain was cultured in the corresponding medium for 2-3 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibac...

Embodiment 3

[0063] Determination of Cellulose and Protein Decomposing Ability of Single Bacteria and Composite Bacteria

[0064] Bacillus subtilis can synthesize enzymes such as protease, cellulase, α-amylase and lipase by itself, which can effectively degrade organic substances such as cellulose and protein in organic materials; Bacillus licheniformis can produce a variety of active enzymes, Such as protease, cellulase, amylase, lipase, pectinase, glucanase, etc., and can also produce a variety of enzymatic factors; At ~50°C, it can secrete a large amount of cellulase, and quickly decompose organic matter such as straw, livestock and poultry manure, and peat into organic matter; Aspergillus oryzae is a strain that produces complex enzymes. In addition to protease, it can also produce cellulase, Amylase, glucoamylase, phytase, etc. Trichoderma viride is one of the strains with the highest cellulase activity, and the cellulase produced can degrade organic substances, and the effect is ve...

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Abstract

The invention discloses a multifunctional agricultural microbial agent as well as an application method and effects thereof. The microbial agent is compounded from 1-3 parts by weight of bacillus subtilis, 0.5-2 parts by weight of Bacillus licheniformis,1-2 parts by weight of Sporotrichum thermophile, 0.5-1 part by weight of aspergillus oryzae and 0.5-3 parts by weight of trichoderma viride, and has the effective viable count of 5*10<8> per g. The microbial agent has a plurality of functions, is capable of decomposing organic materials and deodorizing, and can be applied to preventing diseases and increasing production in crop planting. The organic materials refer to organic waste materials rich in cellulose and protein such as excrements of livestock, crop straws, sludge, compostable household refuse and spent mushroom compost. The microbial agent is applied to treating the organic materials, and has better effects than the control without the microbial agent in the aspects of microbial content, available nutrient, C/N ratio, odor and fermentation period; the period of fermentation by using the microbial agent is 15 days, while the period of fermentation of the control is 32 days; and if the microbial agent is applied to cucumber planting, the disease control efficiency can be 60.20% and the production increase per mu can be 15.6%.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms in crop cultivation and organic waste treatment, and relates to a multifunctional agricultural microbial bacterial agent and a use method thereof. Background technique [0002] Agricultural microbial agent is a live bacterial preparation processed by target microorganisms or beneficial bacteria after industrialization and multiplication. It is widely used in the cultivation of crops, the decomposition of organic materials and the improvement of the environment. According to the types of microorganisms contained or functional characteristics It can be divided into rhizobium bacteria, nitrogen-fixing bacteria, phosphorus-solubilizing microorganisms, silicate microorganisms, photosynthetic bacteria, organic material decomposing agents, growth-promoting bacteria and mycorrhizal bacteria. [0003] The total organic waste discharge in my country is 41.3-43.4×10 8 t, of which the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/04A01P1/00A01P3/00C05F17/00C12R1/125C12R1/10C12R1/69C12R1/885C12R1/645
CPCY02W30/40
Inventor 何宗均梁海恬赵琳娜田阳
Owner 天蓝地沃(天津)农业科技有限公司
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