109 results about "Elastase" patented technology

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle composition and a preparation method and application thereof as well as cosmetics, the anti-wrinkle composition comprises a flos magnoliae extract and an emblic leafflower fruit extract, and the mass ratio of the flos magnoliae extract to the emblic leafflower fruit extract is (0.1-8): (0.5-10); through the synergistic effect of flos magnoliae and fructus phyllanthi contained in the composition, the composition not only has good elastinase inhibition ability, but also has excellent antioxidant ability, so that the composition has good elastinase inhibition ability and good free radical scavenging ability, and further achieves good anti-wrinkle performance.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The invention belongs to the technical field of plant fermentation, and relates to fructus arctii fermentation liquor with soothing and anti-inflammatory effects as well as a preparation method and application of the fructus arctii fermentation liquor. The fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16, and the fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16. According to the invention, after the fructus arctii is used as a raw material and is fermented by the schizosaccharomyces pombe, the hyaluronidase inhibition capability, the elastase inhibition capability and the DPPH free radical scavenging capability of the fermentation product can be improved, so that the fermentation product with better soothing, anti-inflammatory and anti-oxidation capabilities is obtained, and the requirements of the market on efficient, natural and safe skin care components are met.

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of anti-aging of cosmetics. The anti-aging composition is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of roxburgh rose peptide, 1.1 to 1.3 percent of hydrolyzed abelmoschus esculentus extract, 0.7 to 0.9 percent of ligusticum wallichii root extract, 1.5 to 1.7 percent of silybum marianum seed oil and the balance of auxiliary materials, the roxburgh rose peptide is prepared by a two-step enzymolysis method, and the hydrolyzed abelmoschus esculentus extract is prepared by a two-step enzymolysis method. The ligusticum wallichii root extract is obtained through supercritical CO2 extraction. Performance tests show that the composition can significantly inhibit the activity of elastase, the inhibition rate reaches 70% or above, the composition has excellent DPPH free radical scavenging capacity, the scavenging rate reaches 80% or above, all the components are synergistic, skin aging can be effectively delayed, and the composition has good application prospects.

The invention discloses a beautifying essence which comprises the following components in percentage by weight: 1% of bifidus yeast fermentation liquor, 3% of boine, 2% of nicotinamide, 0.3% of gallnut extract, 1% of purslane extract, 0.3% of allantoin, 0.2% of centella asiatica extract, 0.3% of tocopherol extract, 0.5% of aesculus chinensis extract, 2% of vitamin E, 0.05% of xanthan gum, 0.05% of sodium hyaluronate, 0.2% of carbomer, 0.5% of p-hydroxyacetophenone, 0.5% of 1, 2-propylene glycol and the balance of water. The cleaning agent is prepared from, by weight, 0.5% of 1, 2-hexanediol, 5% of glycerin, 4% of PEG / PPG-14 / 7 dimethyl ether, 2.2% of glycosylglycerol, 0.2% of tetrahydroxypropyl ethylenediamine, 3% of polyglycerol-3, 0.05% of PDRN powder and the balance water. Effect evaluation test results show that the beauty essence can inhibit the activity of elastase and increase the content of type I collagen in human primary fibroblasts, so that the firming and anti-wrinkle effects are achieved.

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle firming composition and application thereof and cosmetics, the anti-wrinkle firming composition comprises an oregano leaf extract and a tribulus terrestris fruit extract, and the mass ratio of the oregano leaf extract to the tribulus terrestris fruit extract is (1-10): (5-15). Through the synergistic effect between the origanum vulgare leaf extract and the tribulus terrestris fruit extract, good anti-wrinkle and firming effects can be effectively and synergistically provided for skin in the aspects of free radical removal and elastinase inhibition capability improvement, in addition, after the achillea millefolium extract is further added into the composition, the anti-wrinkle and firming effects are further achieved, and the anti-wrinkle and firming effects are further achieved. The anti-wrinkle and firming capability of the composition can be further improved.

The invention belongs to the field of functional polypeptides, and discloses an elastinase inhibitory peptide and a preparation method thereof. The elastase inhibitory peptide comprises nine types of oligopeptides from crayfish, and the amino acid sequences of the oligopeptides are respectively GASGPAGPRGPVGVA, IDGF, GAAGPAGPRGPAGPA, SGPAGPA, GPAGPRGPVGVA, SGPAGPRGPVGVA, VGPQGASGPLGPA and IGPA. The invention further discloses a preparation method of the elastase inhibitory peptide. The elastinase inhibitory peptide disclosed by the invention can be chemically synthesized and can also be directionally prepared from fish gelatin enzymolysis products. The elastase inhibitory peptide disclosed by the invention has good binding force with elastase and has remarkable elastase inhibitory activity.

The invention discloses algin with anti-photoaging activity as well as a preparation method and application of the algin, and belongs to the technical field of deep processing of seaweed. The molecular weight of the algin is 10-350kDa, the total sugar content is 40wt%-80wt%, the reducing sugar content is 3wt%-16wt%, and the uronic acid content is 40wt%-80wt%. The preparation method of the algin specifically comprises the following steps: (1) degrading dielectric barrier discharge plasma; and (2) dialyzing and freeze-drying. The algin can significantly improve the inhibition rate of elastase and the cell survival rate of HaCaT cells after ultraviolet irradiation, has significant anti-photoaging activity, and can be used for developing anti-photoaging functional foods and skin care products. Meanwhile, the preparation method adopting the dielectric barrier discharge plasma is simple and convenient to operate, rapid, efficient, green, environment-friendly and free of addition of exogenous chemical substances, and the molecular weight of the algin can be remarkably reduced, so that the additional value of the algin is improved, the application range of the algin is widened, and the algin has a good application prospect.

The invention relates to a tea-collagen composition with whitening and anti-aging effects as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a tea-collagen composition with whitening and anti-aging effects. The tea-collagen composition is prepared from epigallocatechin gallate, collagen IV and collagen VII. According to the invention, the safety and anti-oxidation characteristics of EGCG are utilized, and collagen IV and collagen VII are combined to stabilize a cell basement membrane in the form of'glue ', so that the collagen is strengthened, and further the whitening and anti-aging effects are achieved. Meanwhile, experiments prove that the EGCG can synergistically improve the elastase inhibition ratio of the collagen IV and the collagen VII, conversely, the collagen IV and the collagen VII can also synergistically improve the tyrosinase inhibition ratio of the EGCG, and the tea-collagen composition has the whitening and anti-aging effects due to the complementation of the EGCG, the collagen IV and the collagen VII.

The invention discloses a whitening, anti-wrinkle, firming and soothing multi-effect composition, which is prepared from the following raw material components: nicotinamide, hydroxypropyl tetrahydropyrantriol, p-hydroxyacetophenone, glycerol, 1, 2-pentanediol, 1, 2-hexanediol, a barley extract composition, panthenol, a radix gentianae extract composition, an oat kernel extract composition and the balance of water. The barley extract, the radix gentianae extract and the oat kernel extract are added into the whitening, anti-wrinkle, firming and soothing multi-effect composition, so that melanosome can be inhibited from being transferred to the skin surface, DNA damage caused by UV is repaired, photoimmunosuppression is intervened, elastase and hyaluronidase are inhibited, and the whitening, anti-wrinkle, firming and soothing multi-effect composition has the advantages that the whitening, anti-wrinkle, firming and soothing functions are realized; the face cream prepared from the multi-effect composition for whitening, resisting wrinkles, tightening and relieving has the effects of whitening, resisting wrinkles, tightening and relieving.

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

The present invention discloses an adenosine firming and anti-wrinkle composition and its application, specifically belonging to the field of cosmetic preparations; the composition comprises: adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract; the firming and anti-wrinkle composition provided by the present invention can effectively inhibit elastase synthesis and has excellent firming and anti-wrinkle effects; the firming and anti-wrinkle essence containing the firming and anti-wrinkle composition provided by the present invention has excellent firming and anti-wrinkle effects.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

The invention belongs to the field of chemical analysis and testing, and particularly relates to a fluorescent probe for preparing a reagent for detecting elastase as well as a synthesis method and application of the fluorescent probe. The preparation method comprises the following steps: dissolving 2-dicyanomethylene-3-cyano-4, 5, 5-trimethyl-2, 5-dihydrofuran and 6-aminopyridine-3-formaldehyde in ethanol, and heating and refluxing a reaction solution at 50 DEG C under the acidic condition of ammonium acetate to obtain an intermediate TCF-N-NH2; and dissolving the intermediate TCF-N-NH2 and pentafluoropropionic anhydride in dichloromethane, stirring the reaction solution at the temperature of 0 DEG C to room temperature under the alkaline condition of pyridine, and separating and purifying the obtained crude product through thin layer chromatography to obtain the final orange product TCF-NF. The fluorescent probe obtained by the invention can quickly recognize elastase in deionized water.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention belongs to the technical field of skin care products, and relates to a plant fermentation skin care composition and a preparation method thereof. The composition is prepared from the following raw materials in percentage by weight: 0.5 to 1.5 percent of silybum marianum extract, 70 to 80 percent of rosemary fermentation essence and 20 to 30 percent of turmeric fermentation essence. The skin care composition inhibits skin aging through the following ways: 1, inhibiting KLK5 target expression, repairing skin barrier, inhibiting various skin inflammations, and preventing skin cell aging; 2, TIMP2 target expression is enhanced, and IV type collagen synthesis is promoted. And 3, the expression of an MMP3 target spot is inhibited, so that the degradation of various collagens such as III, IV and V types is reduced. And 4. Resisting oxidation, inhibiting activity of elastase, collagenase and hyaluronidase, inhibiting degradation of elastase, collagenase and hyaluronidase, and relieving skin aging.

The invention provides an NPV linker sequence-based GD2 targeting antibody coupling drug, a preparation method and an application, and belongs to the technical field of biological medicines. According to the invention, an Asn-Pro-Val (NPV) sequence sensitive to neutrophil elastase is taken as a linker, and MMAE and Hu 3F8 are coupled by using a cysteine coupling technology, so that the antibody conjugate Hu 3F8-NPV-MMAE is obtained. The antibody conjugate shows good tumor cell killing ability, can significantly reduce the tumor volume, and is high in safety. And after passing through intercellular, the elastase of the antibody conjugate is split to release MMAE to play a role, so that the bystander effect can be enhanced, the curative effect of ADC can be improved, the drug-resistant mechanism related to ADC endocytosis and intracellular processing can be avoided, the enzyme specific splitting of Linker can be improved, and the toxic and side effects of ADC can be reduced.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The present application relates to a kind of composition with whitening and anti-aging and its application, belong to the field of cosmetics.The composition with whitening and anti-aging of the present application includes the following mass parts of components: tetrahydro magnolia alcohol: 0.1-10 parts;Phytol: 1-5 parts;Resveratrol: 0.1-5 parts.The composition with whitening and anti-aging of the present application has significant inhibitory effect on tyrosinase and elastase, and has excellent whitening, anti-aging efficacy.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

A cosmetic composition is provided, which comprises a carrier and a Patchouli stem extract, in particular an extract from exhausted Patchouli stems. This composition provides anti-oxidant, anti-elastase, anti-hyaluronase, and anti-tyrosinase activities.

The invention relates to the technical field of animal model construction, and discloses a mouse abdominal aortic aneurysm model construction method. The construction method comprises the step that in a 18-month-old mouse, a sterile cotton piece soaked with elastinase is used for wrapping the kidney subabdominal aorta to induce formation of the abdominal aortic aneurysm. The mouse model can better reflect the degenerative change of the aortic wall of an elderly patient, better fits the clinical manifestation in the development speed and severity of aortic aneurysm, can more stably simulate the development process of the abdominal aortic aneurysm of the elderly patient, can be used as an effective tool for studying senescence-related aneurysm diseases, and has a wide application prospect. The method is helpful for revealing acceleration and aggravation processes of the elderly individuals in pathological development, and provides a more targeted theoretical basis and experimental support for designing and evaluating a new treatment method.

The application discloses a polypeptide-wrapped anti-wrinkle composition containing camellia sinensis leaf extract and application thereof, which comprises component A, component B and component C; the component A comprises palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, polyol A and caprylic / capric triglyceride; the component B comprises PPG-13-decyltetradecin polyether-24 and polyol B; and the component C comprises water and camellia sinensis leaf extract. The application firstly solves the solubility problem of palmitoyl peptides, and secondly solves the compatibility problem of camellia sinensis leaf extract and palmitoyl peptides by low-energy emulsification technology, so that a transparent flowing liquid is obtained, which is more stable, safer, more effective, more convenient to apply, can promote collagen production and inhibit the activity of elastase, and better achieves the effects of improving wrinkles and delaying aging.

The invention belongs to the technical field of biology, and particularly relates to a chronic obstructive pulmonary disease mouse model and a construction method and application thereof. The construction method comprises the following steps: treating a mouse by adopting biofuel smoke exposure, and inducing the mouse in combination with elastase atomization, so as to construct the chronic obstructive pulmonary disease mouse model. According to the method, through a synergistic induction strategy of combining biofuel smoke with elastase atomization, the induction time of a traditional COPD model can be greatly shortened from 6 months to 4 months, and the method is superior to the prior art in the aspects of phenotypic stability, reality correlation and the like, and an ideal model closer to clinic is provided for etiological research, mechanism exploration and drug screening research and development of COPD.

The invention relates to the technical field of cosmetics, in particular to a tightening and brightening composition containing a white truffle extract and application of the tightening and brightening composition. The composition comprises a white truffle fungus extract, a dendrobium devonianum stem extract, a spirulina maxima extract and a pyracantha fortuneana fruit extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastase and collagenase, has a certain whitening effect, and is mild and non-irritating.

The invention provides a method for constructing an emphysema mouse airway candida colonization model, and belongs to the technical field of candida colonization models.The method comprises the following steps that 1, an emphysema mouse model is constructed, including tobacco smoke exposure and elastase titration; and 2, establishing the pulmonary emphysema mouse Candida albicans airway colonization model, including preparation of a Candida albicans suspension and airway Candida colonization. The genomes of mice are closer to those of human beings than those of other animals, the mice have relatively wide gene, protein sequence and antibody availability, and the modeling cost of the mice is low, so that the mice are more suitable for experimental research of emphysema. Therefore, the construction method of the model is closer to a natural generation mode, is simple and convenient, can better simulate disease characteristics of candida albicans airway colonization in COPD, and creates conditions for further researching whether candida albicans airway colonization has a certain relationship with occurrence and development of COPD and acute exacerbation thereof.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The present invention belongs to the technical field of active substance extraction, and specifically relates to a low-fat, low-cost, high-purity and high-quality elastin peptide and a preparation method thereof. The present invention uses a mixture of alkali solution and sodium carbonate solution, the overall concentration is low, it is more environmentally friendly, and the degreasing effect is better using microwave heating technology. The present invention uses microsphere immobilized protease, and the enzyme preparation can be fully recovered and reused multiple times, which greatly reduces production costs. The elastase hydrolysate is separated, purified and refined using ion exchange resin series chromatography technology to efficiently remove other impurities, and the prepared elastin peptide has a higher purity. The present invention uses high-voltage pulsed electric field technology (PEF) for sterilization, which has a better sterilization effect, avoids the influence of traditional heat sterilization methods on the color and taste of the product, and better maintains the quality of the elastin peptide.