149 results about "Elastase" patented technology

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle composition and a preparation method and application thereof as well as cosmetics, the anti-wrinkle composition comprises a flos magnoliae extract and an emblic leafflower fruit extract, and the mass ratio of the flos magnoliae extract to the emblic leafflower fruit extract is (0.1-8): (0.5-10); through the synergistic effect of flos magnoliae and fructus phyllanthi contained in the composition, the composition not only has good elastinase inhibition ability, but also has excellent antioxidant ability, so that the composition has good elastinase inhibition ability and good free radical scavenging ability, and further achieves good anti-wrinkle performance.

The invention provides an anti-aging and whitening pyracantha fortuneana fruit extract prepared by adopting a supramolecular solvent and a preparation method of the pyracantha fortuneana fruit extract, and relates to the technical field of biological extraction. The preparation method comprises the following steps: by taking betaine as a hydrogen bond acceptor and 1, 3-propylene glycol as a hydrogen bond donor, mixing and self-assembling the betaine and the 1, 3-propylene glycol to form a supramolecular solvent; and the pyracantha fortuneana fruits are extracted by adopting the supramolecular solvent. The pyracantha fortuneana fruit active ingredients are extracted by adopting the supramolecular solvent which is harmless to the skin, so that the irritation of the solvent to the skin can be avoided, the supramolecular solvent can be specifically combined with the active ingredients in the pyracantha fortuneana fruit, and the purity and activity of the active ingredients are improved. The pyracantha fortuneana fruit extract extracted by adopting the supramolecular solvent has better oxidation reduction resistance; generation of non-enzymatic glycosylation intermediate products is inhibited, and saccharification and aging resistance are facilitated; the red blood cell oxidative damage is improved, and the effects of resisting oxidation and relieving are achieved; the whitening mask has an inhibiting effect on elastinase and tyrosinase, and can resist aging, whiten skin, relieve facial pigmentation and brighten the skin.

The invention relates to the technical field of deep processing of paeonia ostii, in particular to paeonia ostii peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis as well as a preparation method and application of the paeonia ostii peptide. The paeonia ostii flower peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis is prepared through water bath, homogenization, alkali extraction and acid precipitation pretreatment, three times of enzymolysis through compound enzyme of acid protease, cellulase and pectinase and alkaline protease and solid-liquid separation, operation is easy, the requirement for equipment is low, and the paeonia ostii flower peptide is suitable for industrial large-scale production. The prepared paeonia ostii flower peptide has good inhibition capability on tyrosinase, hyaluronidase and elastinase, and also has excellent anti-saccharification and anti-inflammatory effects. Besides, five paeonia ostii active peptide fragments are screened out, are high in activity and non-toxic, can be combined with tyrosinase, hyaluronidase, MMP-1, MMP-3 and COX-2 receptor protein, and have the effects of whitening, moisturizing, tightening and promoting collagen synthesis.

The invention relates to the technical field of tissue isolation, and particularly discloses a high-activity intestinal crypt and an isolation method and application thereof. The separation method comprises the following steps: S1, taking fresh intestinal tracts, cleaning contents, and scraping mucous membrane layer tissues to obtain intestinal mucous membrane tissues; s2, shearing intestinal mucosa layer tissues, mixing the intestinal mucosa layer tissues with the separation liquid after cleaning, and digesting and dissociating to obtain intestinal mucosa tissue digestive juice; s3, centrifuging the intestinal mucosa tissue digestive juice, removing supernate, mixing the supernate with the protective liquid to obtain intestinal crypt mixed liquid, filtering, centrifuging, and taking precipitate to obtain high-activity intestinal crypts; wherein the separation liquid comprises collagenase I, collagenase II, collagenase IV, elastase, DNA enzyme I and a ROCK inhibitor; the protection liquid comprises an RPMI1640 culture medium, antibiotics, fetal calf serum, bovine serum albumin and a ROCK inhibitor. The technical scheme provided by the invention aims to shorten the intestinal tract crypt separation time and improve the cell viability.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The invention belongs to the technical field of plant fermentation, and relates to fructus arctii fermentation liquor with soothing and anti-inflammatory effects as well as a preparation method and application of the fructus arctii fermentation liquor. The fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16, and the fructus arctii fermentation liquid is obtained by fermenting fructus arctii through schizosaccharomyces pombe JM2-16. According to the invention, after the fructus arctii is used as a raw material and is fermented by the schizosaccharomyces pombe, the hyaluronidase inhibition capability, the elastase inhibition capability and the DPPH free radical scavenging capability of the fermentation product can be improved, so that the fermentation product with better soothing, anti-inflammatory and anti-oxidation capabilities is obtained, and the requirements of the market on efficient, natural and safe skin care components are met.

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of anti-aging of cosmetics. The anti-aging composition is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of roxburgh rose peptide, 1.1 to 1.3 percent of hydrolyzed abelmoschus esculentus extract, 0.7 to 0.9 percent of ligusticum wallichii root extract, 1.5 to 1.7 percent of silybum marianum seed oil and the balance of auxiliary materials, the roxburgh rose peptide is prepared by a two-step enzymolysis method, and the hydrolyzed abelmoschus esculentus extract is prepared by a two-step enzymolysis method. The ligusticum wallichii root extract is obtained through supercritical CO2 extraction. Performance tests show that the composition can significantly inhibit the activity of elastase, the inhibition rate reaches 70% or above, the composition has excellent DPPH free radical scavenging capacity, the scavenging rate reaches 80% or above, all the components are synergistic, skin aging can be effectively delayed, and the composition has good application prospects.

The invention discloses a beautifying essence which comprises the following components in percentage by weight: 1% of bifidus yeast fermentation liquor, 3% of boine, 2% of nicotinamide, 0.3% of gallnut extract, 1% of purslane extract, 0.3% of allantoin, 0.2% of centella asiatica extract, 0.3% of tocopherol extract, 0.5% of aesculus chinensis extract, 2% of vitamin E, 0.05% of xanthan gum, 0.05% of sodium hyaluronate, 0.2% of carbomer, 0.5% of p-hydroxyacetophenone, 0.5% of 1, 2-propylene glycol and the balance of water. The cleaning agent is prepared from, by weight, 0.5% of 1, 2-hexanediol, 5% of glycerin, 4% of PEG / PPG-14 / 7 dimethyl ether, 2.2% of glycosylglycerol, 0.2% of tetrahydroxypropyl ethylenediamine, 3% of polyglycerol-3, 0.05% of PDRN powder and the balance water. Effect evaluation test results show that the beauty essence can inhibit the activity of elastase and increase the content of type I collagen in human primary fibroblasts, so that the firming and anti-wrinkle effects are achieved.

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle firming composition and application thereof and cosmetics, the anti-wrinkle firming composition comprises an oregano leaf extract and a tribulus terrestris fruit extract, and the mass ratio of the oregano leaf extract to the tribulus terrestris fruit extract is (1-10): (5-15). Through the synergistic effect between the origanum vulgare leaf extract and the tribulus terrestris fruit extract, good anti-wrinkle and firming effects can be effectively and synergistically provided for skin in the aspects of free radical removal and elastinase inhibition capability improvement, in addition, after the achillea millefolium extract is further added into the composition, the anti-wrinkle and firming effects are further achieved, and the anti-wrinkle and firming effects are further achieved. The anti-wrinkle and firming capability of the composition can be further improved.

The invention belongs to the technical field of cosmetics, and particularly relates to a natural-source composition with a function of inhibiting multiple types of matrix metalloproteinases of skin and application of the natural-source composition. The composition is prepared from the following components: herba cistanche powder, American ginseng powder, a red-knees herb extract and a creeping rostellularia herb extract. The composition has the effects of inhibiting expression of multiple types of matrix metalloproteinases in skin immortalized keratinocytes, improving elastin synthesis and inhibiting elastase decomposition, and has no obvious side effects. The composition disclosed by the invention can be used for preparing various cosmetics, functional foods and health-care products related to skin aging resistance, and has a wide application prospect.

The invention provides a prinsepia utilis royle extract as well as a preparation method and application thereof, and the preparation method comprises the following steps: picking prinsepia utilis royle seeds, removing epicarp from the prinsepia utilis royle seeds, dehydrating to obtain dried kernels, preparing prinsepia utilis royle crude oil from the kernels by a subcritical extraction method, refining the crude oil by a deodorization vacuum process to obtain refined non-winterized prinsepia utilis royle oil, and drying the refined non-winterized prinsepia utilis royle oil to obtain the prinsepia utilis royle extract. And performing cold tank winterization treatment on the refined non-winterized prinsepia utilis royle oil to obtain the refined winterized prinsepia utilis royle oil. The prinsepia utilis royle extract obtained by the embodiment of the invention has good effects of resisting oxidation, inhibiting tyrosinase, inhibiting hyaluronidase, inhibiting elastinase, repairing zebra fish and the like.

The invention discloses application of a compound in preparation of a skin and / or scalp care product, the structural formula of the compound is # imgabs0, and compared with retinoic acid, the compound provided by the invention has higher cell safety concentration and lower irritation, has a more excellent effect in the aspect of cell damage repair, and has a better cell moisturizing effect; compared with the retinyl phytosphingosine, the compound provided by the invention has a better effect in the aspects of inhibiting the activity of elastase and improving type I collagen; compared with retinoyl phytosphingosine, the compound provided by the invention shows a better effect in the aspects of resisting lipid peroxidation and inhibiting malassezia reproduction, and also has better stability. The compound provided by the invention has good application value in the field of preparing cosmetics, medicines and health care products with corresponding effects or indications.

The invention belongs to the field of functional polypeptides, and discloses an elastinase inhibitory peptide and a preparation method thereof. The elastase inhibitory peptide comprises nine types of oligopeptides from crayfish, and the amino acid sequences of the oligopeptides are respectively GASGPAGPRGPVGVA, IDGF, GAAGPAGPRGPAGPA, SGPAGPA, GPAGPRGPVGVA, SGPAGPRGPVGVA, VGPQGASGPLGPA and IGPA. The invention further discloses a preparation method of the elastase inhibitory peptide. The elastinase inhibitory peptide disclosed by the invention can be chemically synthesized and can also be directionally prepared from fish gelatin enzymolysis products. The elastase inhibitory peptide disclosed by the invention has good binding force with elastase and has remarkable elastase inhibitory activity.

The invention discloses a multi-enzyme preparation for industrial cell dissociation as well as a use method and application thereof, and belongs to the technical field of extraction of seed cells for industrial culture of meat. According to the invention, a multi-enzyme preparation composed of a component 1 and a component 2 is adopted; the component 1 comprises neutral protease, hyaluronidase and elastase; and the component 2 comprises matrix metalloproteinase, papain and plasmin. The invention also discloses a preparation method and application of the multienzyme preparation. The reasonable enzyme ratio and the optimized treatment conditions are beneficial to protecting the integrity of cell membranes, maintaining high cell activity and remarkably improving the dissociation efficiency and the cell recovery amount; the complex enzyme preparation is high in adaptability, can be used for various tissue types and dissociation requirements, and is convenient for standardized and large-scale production; in combination with automatic dissociation equipment, the compound enzyme preparation can realize efficient and stable industrial operation, and provides important technical support and wide application prospects for the fields of meat culture industry, tissue engineering, cell therapy and the like.

The invention discloses algin with anti-photoaging activity as well as a preparation method and application of the algin, and belongs to the technical field of deep processing of seaweed. The molecular weight of the algin is 10-350kDa, the total sugar content is 40wt%-80wt%, the reducing sugar content is 3wt%-16wt%, and the uronic acid content is 40wt%-80wt%. The preparation method of the algin specifically comprises the following steps: (1) degrading dielectric barrier discharge plasma; and (2) dialyzing and freeze-drying. The algin can significantly improve the inhibition rate of elastase and the cell survival rate of HaCaT cells after ultraviolet irradiation, has significant anti-photoaging activity, and can be used for developing anti-photoaging functional foods and skin care products. Meanwhile, the preparation method adopting the dielectric barrier discharge plasma is simple and convenient to operate, rapid, efficient, green, environment-friendly and free of addition of exogenous chemical substances, and the molecular weight of the algin can be remarkably reduced, so that the additional value of the algin is improved, the application range of the algin is widened, and the algin has a good application prospect.

The invention relates to a tea-collagen composition with whitening and anti-aging effects as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a tea-collagen composition with whitening and anti-aging effects. The tea-collagen composition is prepared from epigallocatechin gallate, collagen IV and collagen VII. According to the invention, the safety and anti-oxidation characteristics of EGCG are utilized, and collagen IV and collagen VII are combined to stabilize a cell basement membrane in the form of'glue ', so that the collagen is strengthened, and further the whitening and anti-aging effects are achieved. Meanwhile, experiments prove that the EGCG can synergistically improve the elastase inhibition ratio of the collagen IV and the collagen VII, conversely, the collagen IV and the collagen VII can also synergistically improve the tyrosinase inhibition ratio of the EGCG, and the tea-collagen composition has the whitening and anti-aging effects due to the complementation of the EGCG, the collagen IV and the collagen VII.

The invention discloses a whitening, anti-wrinkle, firming and soothing multi-effect composition, which is prepared from the following raw material components: nicotinamide, hydroxypropyl tetrahydropyrantriol, p-hydroxyacetophenone, glycerol, 1, 2-pentanediol, 1, 2-hexanediol, a barley extract composition, panthenol, a radix gentianae extract composition, an oat kernel extract composition and the balance of water. The barley extract, the radix gentianae extract and the oat kernel extract are added into the whitening, anti-wrinkle, firming and soothing multi-effect composition, so that melanosome can be inhibited from being transferred to the skin surface, DNA damage caused by UV is repaired, photoimmunosuppression is intervened, elastase and hyaluronidase are inhibited, and the whitening, anti-wrinkle, firming and soothing multi-effect composition has the advantages that the whitening, anti-wrinkle, firming and soothing functions are realized; the face cream prepared from the multi-effect composition for whitening, resisting wrinkles, tightening and relieving has the effects of whitening, resisting wrinkles, tightening and relieving.

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

The present invention discloses an adenosine firming and anti-wrinkle composition and its application, specifically belonging to the field of cosmetic preparations; the composition comprises: adenosine, sunflower sprout extract, hydrolyzed pansy extract, and Artemia extract; the firming and anti-wrinkle composition provided by the present invention can effectively inhibit elastase synthesis and has excellent firming and anti-wrinkle effects; the firming and anti-wrinkle essence containing the firming and anti-wrinkle composition provided by the present invention has excellent firming and anti-wrinkle effects.

The invention relates to a calf bone marrow hydrolysate liposome freeze-dried powder and its application in hematopoiesis-promoting and anti-aging products, belonging to the field of biotechnology. The calf bone marrow hydrolysate liposome freeze-dried powder is a product obtained by using calf bone marrow as a raw material, and enzymolyzing the product step by step with pancreatic lipase, alkaline phosphatase, trypsin, elastase, and ficin to obtain an enzymolyzate below 3 kDa, and then coating the product with liposomes. The product is rich in multiple small and medium molecule nutritional active substances, growth factors, and small molecule peptide components. The combination of each component can enhance the efficacy, can more effectively promote hematopoiesis, and also has excellent antioxidant ability, removes free radicals in the body, reduces the damage of oxidation reactions to cells and tissues, and effectively delays the aging process.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

The invention belongs to the field of chemical analysis and testing, and particularly relates to a fluorescent probe for preparing a reagent for detecting elastase as well as a synthesis method and application of the fluorescent probe. The preparation method comprises the following steps: dissolving 2-dicyanomethylene-3-cyano-4, 5, 5-trimethyl-2, 5-dihydrofuran and 6-aminopyridine-3-formaldehyde in ethanol, and heating and refluxing a reaction solution at 50 DEG C under the acidic condition of ammonium acetate to obtain an intermediate TCF-N-NH2; and dissolving the intermediate TCF-N-NH2 and pentafluoropropionic anhydride in dichloromethane, stirring the reaction solution at the temperature of 0 DEG C to room temperature under the alkaline condition of pyridine, and separating and purifying the obtained crude product through thin layer chromatography to obtain the final orange product TCF-NF. The fluorescent probe obtained by the invention can quickly recognize elastase in deionized water.

The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of tumor infiltrating lymphocytes. Keratinase modified by myristoyl coenzyme A cooperates with fibronectin and elastase, so that tumor cells quickly fall off from tumor blocks, and loose tissues are subjected to low-temperature enzymolysis by protease-K, so that the phenotype and activity of tumor infiltrating lymphocytes can be protected; an eicosapentaenoic acid solution is mixed with polysorbate, ultrapure water and a culture medium matrix to obtain an emulsion mixed solution, the emulsion mixed solution is subjected to rotary evaporation to remove a solvent, and the amplification-promoting nanoparticles obtained after freeze drying can make tumor infiltrating lymphocytes ingest effective components more quickly; the tumor infiltrating lymphocytes extracted by the invention are high in activity and rapid in proliferation and growth, still have high cell activity after cryopreservation, and have good anti-breast tumor activity.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention belongs to the technical field of skin care products, and relates to a plant fermentation skin care composition and a preparation method thereof. The composition is prepared from the following raw materials in percentage by weight: 0.5 to 1.5 percent of silybum marianum extract, 70 to 80 percent of rosemary fermentation essence and 20 to 30 percent of turmeric fermentation essence. The skin care composition inhibits skin aging through the following ways: 1, inhibiting KLK5 target expression, repairing skin barrier, inhibiting various skin inflammations, and preventing skin cell aging; 2, TIMP2 target expression is enhanced, and IV type collagen synthesis is promoted. And 3, the expression of an MMP3 target spot is inhibited, so that the degradation of various collagens such as III, IV and V types is reduced. And 4. Resisting oxidation, inhibiting activity of elastase, collagenase and hyaluronidase, inhibiting degradation of elastase, collagenase and hyaluronidase, and relieving skin aging.

The invention provides an NPV linker sequence-based GD2 targeting antibody coupling drug, a preparation method and an application, and belongs to the technical field of biological medicines. According to the invention, an Asn-Pro-Val (NPV) sequence sensitive to neutrophil elastase is taken as a linker, and MMAE and Hu 3F8 are coupled by using a cysteine coupling technology, so that the antibody conjugate Hu 3F8-NPV-MMAE is obtained. The antibody conjugate shows good tumor cell killing ability, can significantly reduce the tumor volume, and is high in safety. And after passing through intercellular, the elastase of the antibody conjugate is split to release MMAE to play a role, so that the bystander effect can be enhanced, the curative effect of ADC can be improved, the drug-resistant mechanism related to ADC endocytosis and intracellular processing can be avoided, the enzyme specific splitting of Linker can be improved, and the toxic and side effects of ADC can be reduced.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.