78 results about "Elastase" patented technology

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of anti-aging of cosmetics. The anti-aging composition is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of roxburgh rose peptide, 1.1 to 1.3 percent of hydrolyzed abelmoschus esculentus extract, 0.7 to 0.9 percent of ligusticum wallichii root extract, 1.5 to 1.7 percent of silybum marianum seed oil and the balance of auxiliary materials, the roxburgh rose peptide is prepared by a two-step enzymolysis method, and the hydrolyzed abelmoschus esculentus extract is prepared by a two-step enzymolysis method. The ligusticum wallichii root extract is obtained through supercritical CO2 extraction. Performance tests show that the composition can significantly inhibit the activity of elastase, the inhibition rate reaches 70% or above, the composition has excellent DPPH free radical scavenging capacity, the scavenging rate reaches 80% or above, all the components are synergistic, skin aging can be effectively delayed, and the composition has good application prospects.

The invention belongs to the technical field of cosmetic production, and discloses an anti-wrinkle firming composition and application thereof and cosmetics, the anti-wrinkle firming composition comprises an oregano leaf extract and a tribulus terrestris fruit extract, and the mass ratio of the oregano leaf extract to the tribulus terrestris fruit extract is (1-10): (5-15). Through the synergistic effect between the origanum vulgare leaf extract and the tribulus terrestris fruit extract, good anti-wrinkle and firming effects can be effectively and synergistically provided for skin in the aspects of free radical removal and elastinase inhibition capability improvement, in addition, after the achillea millefolium extract is further added into the composition, the anti-wrinkle and firming effects are further achieved, and the anti-wrinkle and firming effects are further achieved. The anti-wrinkle and firming capability of the composition can be further improved.

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

The embodiment of the invention discloses citrus peel pectin, citrus peel pectin emulsion and application. The content of the citrus peel pectin is 66-67%, the protein content is 13-14%, and the esterification degree is 72-73%; the sugar comprises the following components in percentage by mole: 58-59% of galacturonic acid, 25-26% of arabinose, 5-6% of galactose, 4-5% of rhamnose, 2-3% of glucose and 2-3% of xylose. The citrus peel pectin provided by the invention has excellent emulsibility; the citrus peel pectin emulsion has excellent emulsion stability, and shows a better encapsulation effect on LBE. The LBE emulsion obtained by encapsulating LBE by using CP has the characteristics of high encapsulation efficiency, low toxicity and high biocompatibility, and the synergistic effect of CP obviously enhances the antioxidant activity of LBE and the activity inhibition rate of tyrosinase, elastase and hyaluronidase, so that the LBE emulsion can be used as a novel anti-aging emulsion formula to be applied to cosmetics, and has a good application prospect. Good application prospects are realized.

The invention provides an NPV linker sequence-based GD2 targeting antibody coupling drug, a preparation method and an application, and belongs to the technical field of biological medicines. According to the invention, an Asn-Pro-Val (NPV) sequence sensitive to neutrophil elastase is taken as a linker, and MMAE and Hu 3F8 are coupled by using a cysteine coupling technology, so that the antibody conjugate Hu 3F8-NPV-MMAE is obtained. The antibody conjugate shows good tumor cell killing ability, can significantly reduce the tumor volume, and is high in safety. And after passing through intercellular, the elastase of the antibody conjugate is split to release MMAE to play a role, so that the bystander effect can be enhanced, the curative effect of ADC can be improved, the drug-resistant mechanism related to ADC endocytosis and intracellular processing can be avoided, the enzyme specific splitting of Linker can be improved, and the toxic and side effects of ADC can be reduced.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The present application relates to a kind of composition with whitening and anti-aging and its application, belong to the field of cosmetics.The composition with whitening and anti-aging of the present application includes the following mass parts of components: tetrahydro magnolia alcohol: 0.1-10 parts;Phytol: 1-5 parts;Resveratrol: 0.1-5 parts.The composition with whitening and anti-aging of the present application has significant inhibitory effect on tyrosinase and elastase, and has excellent whitening, anti-aging efficacy.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

A cosmetic composition is provided, which comprises a carrier and a Patchouli stem extract, in particular an extract from exhausted Patchouli stems. This composition provides anti-oxidant, anti-elastase, anti-hyaluronase, and anti-tyrosinase activities.

The application discloses a polypeptide-wrapped anti-wrinkle composition containing camellia sinensis leaf extract and application thereof, which comprises component A, component B and component C; the component A comprises palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, polyol A and caprylic / capric triglyceride; the component B comprises PPG-13-decyltetradecin polyether-24 and polyol B; and the component C comprises water and camellia sinensis leaf extract. The application firstly solves the solubility problem of palmitoyl peptides, and secondly solves the compatibility problem of camellia sinensis leaf extract and palmitoyl peptides by low-energy emulsification technology, so that a transparent flowing liquid is obtained, which is more stable, safer, more effective, more convenient to apply, can promote collagen production and inhibit the activity of elastase, and better achieves the effects of improving wrinkles and delaying aging.

The invention belongs to the technical field of biology, and particularly relates to a chronic obstructive pulmonary disease mouse model and a construction method and application thereof. The construction method comprises the following steps: treating a mouse by adopting biofuel smoke exposure, and inducing the mouse in combination with elastase atomization, so as to construct the chronic obstructive pulmonary disease mouse model. According to the method, through a synergistic induction strategy of combining biofuel smoke with elastase atomization, the induction time of a traditional COPD model can be greatly shortened from 6 months to 4 months, and the method is superior to the prior art in the aspects of phenotypic stability, reality correlation and the like, and an ideal model closer to clinic is provided for etiological research, mechanism exploration and drug screening research and development of COPD.

The invention relates to the technical field of cosmetics, in particular to a tightening and brightening composition containing a white truffle extract and application of the tightening and brightening composition. The composition comprises a white truffle fungus extract, a dendrobium devonianum stem extract, a spirulina maxima extract and a pyracantha fortuneana fruit extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastase and collagenase, has a certain whitening effect, and is mild and non-irritating.

The invention provides a method for constructing an emphysema mouse airway candida colonization model, and belongs to the technical field of candida colonization models.The method comprises the following steps that 1, an emphysema mouse model is constructed, including tobacco smoke exposure and elastase titration; and 2, establishing the pulmonary emphysema mouse Candida albicans airway colonization model, including preparation of a Candida albicans suspension and airway Candida colonization. The genomes of mice are closer to those of human beings than those of other animals, the mice have relatively wide gene, protein sequence and antibody availability, and the modeling cost of the mice is low, so that the mice are more suitable for experimental research of emphysema. Therefore, the construction method of the model is closer to a natural generation mode, is simple and convenient, can better simulate disease characteristics of candida albicans airway colonization in COPD, and creates conditions for further researching whether candida albicans airway colonization has a certain relationship with occurrence and development of COPD and acute exacerbation thereof.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

This application relates to a species of Pseudomonas aeruginosa. bpsR Genes, BpsR proteins and their applications, engineered bacteria, and drugs, among which bpsR The nucleotide sequence of the gene is shown in SEQ ID No. 1. This invention... bpsR Genes, through regulation in Pseudomonas aeruginosa bpsR Gene expression can regulate the virulence factors of Pseudomonas aeruginosa, such as biofilm production, pyocyanin production, motility, and elastase secretion levels. Furthermore, BpsR protein can be used as a new target for the development of drugs to prevent and treat Pseudomonas aeruginosa, which has important clinical significance for the prevention and control of Pseudomonas aeruginosa infection.

This application belongs to the field of cosmetic manufacturing technology and discloses an anti-wrinkle composition and its preparation method, application, and cosmetics, including Magnolia officinalis flower extract and Phyllanthus emblica fruit extract, wherein the mass ratio of Magnolia officinalis flower extract to Phyllanthus emblica fruit extract is 0.1-8:0.5-10; through the synergistic effect between Magnolia officinalis flower extract and Phyllanthus emblica fruit extract, the composition has both good elastase inhibition ability and excellent antioxidant ability, so that the composition has good elastase inhibition ability and good free radical scavenging ability, thereby achieving good anti-wrinkle performance.

The present application belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition containing lactoferrin and application thereof. The anti-aging composition provided by the present application can realize synergistic effects among four components of the medicinal Fomes fomentarius extract, the Clitoria extract, the Artemisia annua extract and the lactoferrin in terms of anti-aging, achieve excellent elastase inhibition effects, prevent the degradation of elastin in the dermis, effectively reinforce the elastin scaffold, and thus improve the anti-aging effect of the composition. The anti-aging composition applied to the prepared emulsion can improve the skin firmness (R0 change rate), the skin plastic elasticity (R2 change rate) and the facial wrinkles (wrinkle change rate), has the anti-aging effect of improving wrinkle removal and skin tightening and promoting skin elasticity, and does not cause skin irritation, that is, has the characteristics of safety and mildness, has a wide application range, and has high consumer acceptance.

A stable emulsion type anti-aging composition is in the form of an oil in water emulsion has a pleasing tactile feel and includes a blend of compounds including an elastase reducer, for example a C-glycoside derivative also known as hydroxypropyl tetrahydropyrantriol, and two or more compounds selected from the group consisting of cell proliferation agents, for example niacinamide, anti-inflammatory agents, for example Eperua falcata bark extract and collagen production agents, for example Gentiana lutea root extract, and combinations thereof.

This invention relates to the field of cosmetic technology, specifically to an anti-wrinkle cream containing shea butter and its preparation method. The anti-wrinkle cream containing shea butter has the following raw material composition: shea butter, moisturizer, synthetic ester, emulsifier, plant extract, pH adjuster, thickener, and water. This invention uses a combination of shea butter and a specific synthetic ester as a moisturizing base, combined with a specific ratio of nasturtium extract and red algae extract as active ingredients. The resulting anti-wrinkle cream exhibits excellent elastase inhibitory activity and effectively combats wrinkles. Simultaneously, the use of polyglycerol-6 didecanoate and cholesterol polyether-24 as a compound emulsifier system significantly improves the absolute value of the zeta potential, resulting in high product stability and a long shelf life. The preparation method of this invention is simple and suitable for industrial production.

The application discloses a firming and anti-wrinkle cream containing a gallnut extract, and belongs to the field of cosmetics. The gallnut medicine is extracted, and glycosidase is used to hydrolyze tannic acid into gallic acid, so that the hydrolysis conversion efficiency can be controlled, the prepared gallnut extract can better inhibit elastase, promote type I collagen synthesis and reduce the secretion of MMP-1, and thus the prepared cosmetic has better firming and anti-wrinkle effects.

The invention discloses a preparation method of a phallus impudicus extract as well as a product and application of the phallus impudicus extract, and belongs to the technical field of biology. The phallus impudicus extract is prepared by taking phallus impudicus buds as a raw material and adopting a water extraction process, and the mass percentage of polysaccharide in the extract is not less than 70%. The obtained extract has the biological activity effects of resisting oxidation, resisting inflammation, resisting saccharification, inhibiting elastinase, relieving repair activity or promoting skin repair, and experiments prove that the extract is free of cytotoxicity, sensitization and phototoxicity. The phallus impudicus extract is very suitable for sensitive skin and used as a daily skin care product. Development of traditional fungus phallus impudicus is led to the field of high-tech cosmetic raw materials, a complete technical scheme and solid data support are provided for high value-added utilization of phallus impudicus, and the phallus impudicus is wide in application prospect.

The invention discloses compact anti-wrinkle elastin peptide freeze-dried powder and a preparation method thereof, and belongs to the technical field of bioactive peptides. The freeze-dried powder is prepared by taking core tubes and / or throat tubes of bred animals as raw materials through cleaning and impurity removal, crushing and homogenizing, degreasing and impurity removal, composite enzymolysis, enzymolysis inactivation, centrifugal separation, gradient filtration, concentration, sterilization and vacuum freeze drying, and has remarkable elastinase inhibitory activity and tightening and anti-wrinkle effects. The livestock and poultry slaughtering byproducts which are wide in source and low in cost are selected as raw materials, high-value utilization of the livestock and poultry slaughtering byproducts is achieved, the optimized preparation technology solves the problems that in the prior art, enzymolysis efficiency is low, product purity is low, activity is insufficient, and stability is poor, the technology is simple, large-scale production is easy, and the method can be widely applied to the fields of cosmetics, food and health care products.

Methods for inactivating in a cell a mutant allele of the elastase, neutrophil expressed gene (ELANE gene) gene having a mutation associated with severe congenital neutropenia (SCN) or cyclic neutropenia (CyN) and which cell is heterozygous at one or more polymorphic sites selected from the group consisting of: rs10424470, rs4807932, rs10414837, rs376107533, rs3761010, rs10413889, rs3761007, rs10409474, rs3761005, rs351107, rs3761001, rs740021, rs781452480, rs371057361, rs570466264, rs1041904080, rs7250194, rs17216649, rs199720952, rs6510983, rs17223066, rs7255385, rs112639467, rs141213775, rs28591229, rs10469327, rs3834645, rs1683564, rs71335276, and rs8107095, the method comprisingintroducing to the cell a composition comprising:a CRISPR nuclease or a sequence encoding the CRISPR nuclease; anda first RNA molecule comprising a guide sequence portion having 17-30 nucleotides,wherein a complex of the CRISPR nuclease and the first RNA molecule affects a double strand break in the mutant allele of the ELANE genethe method optionally further comprising introduction of a second RNA molecule comprising a guide sequence portion capable of complexing with a CRISPR nuclease, wherein the complex of the second RNA molecule and CRISPR nuclease affects a second double strand break in the ELANE gene.

This invention discloses a method for preparing anthocyanin extract from tree tomato, along with its products and applications, belonging to the field of natural plant extraction and application. The method uses tree tomato peel as raw material, employing an acidic ethanol aqueous solution containing 0.5% (w / v) trifluoroacetic acid as the extraction solvent, at a material-to-liquid ratio of 1:30 (g / mL), at a temperature of 50℃, and for 60 minutes to obtain a high-content anthocyanin extract. This tree tomato anthocyanin extract possesses excellent anti-inflammatory, antioxidant, anti-glycation, elastase-inhibiting, and whitening activities, and exhibits low cytotoxicity, no phototoxicity, and no skin sensitization. The efficient and stable anthocyanin extraction process provided by this invention has high extraction efficiency and good enrichment effect of active ingredients, meeting the requirements for subsequent industrial production. The obtained extract has whitening, anti-aging, and anti-inflammatory effects, providing a high-quality raw material and formulation solution for developing multifunctional and highly safe natural plant-based skincare products.

The invention belongs to the technical field of daily skin care products, and relates to a biological agent with a bionic ECM structure and application of the biological agent in the field of skin aging resistance. The biological agent with a bionic ECM structure is prepared by simulating components of natural ECM of skin and mainly comprises fibronectin rh FN-LN, collagen, glycoprotein, serum albumin, a polyamino acid polysaccharide condensation compound, mannitol, trehalose and auxiliary materials. The auxiliary materials comprise elastin, decapeptide-4 and the like. An elastinase inhibition ratio test and a human skin fibroblast proliferation test prove that the biological agent with the bionic ECM structure has the effects of repairing, resisting aging and improving skin compactness.

The invention discloses application of a citric acid-based biological material as a cosmetic raw material, and belongs to the technical field of cosmetics, and the citric acid-based biological material can be a citric acid-based small molecule or a citric acid-based polymer; the citric acid-based biological material not only has excellent biocompatibility and chemical stability, but also can selectively absorb ultraviolet rays and release blue fluorescence through unique molecular design and structural modification by utilizing the characteristic that the citric acid-based biological material can effectively inhibit generation of melanin in skin, so that the whitening and sunscreen effects are enhanced. In addition, the citric acid-based biological material also shows an inhibiting effect on the activity of elastinase and hyaluronidase, and has the effects of tightening, resisting wrinkles, relieving and removing cutin. The invention provides novel molecular structure design and formula selection for various types of cosmetics for whitening, sunscreen and the like, and provides an innovative thought for efficient and safe application of related products.

The invention relates to application of an Arteridin gene as a biomarker in preparation of a product for auxiliary diagnosis of abdominal aortic aneurysm, clinical specimens and animal experiments find that the expression of Arteridin in occurrence of abdominal aortic aneurysm is remarkably increased, which indicates that Arteridin can be used as the biomarker for auxiliary diagnosis of abdominal aortic aneurysm; the invention also relates to a product for down-regulating the expression of Arterin and an application of the product in preparation of drugs for treating abdominal aortic aneurysm, the product comprises siRNA and shRNA of a targeted Arterin gene and AAV for coding the sequence of the shRNA, and after specific knockdown of Arterin by smooth muscle cells is realized, in an Elatase-induced abdominal aortic mouse model, the expression of Arterin is reduced, and the expression of Arterin is reduced. The diameter of the aneurysm is reduced, the occurrence rate is reduced, the elastin destruction degree is reduced, the expression quantity of vascular remodeling genes MMP2 and MMP9 is reduced, and the difference is obvious compared with that of a control group; the result shows that Arteridin is expected to become a target spot for treating abdominal aortic aneurysm.

The present invention belongs to the field of plant fermentation technology, and relates to a burdock fermentation liquid with soothing and anti-inflammatory effects, a preparation method thereof, and an application thereof. Schizosaccharomyces pombe ) JM2-16 fermentation. The present invention uses burdock as raw material and is fermented with Schizosaccharomyces pombe ( Schizosaccharomyces pombe ) After fermentation, the hyaluronidase inhibition ability, elastase inhibition ability, and DPPH free radical scavenging ability of the fermentation product can be improved, thereby obtaining a fermentation product with better soothing, anti-inflammatory and antioxidant abilities to meet the market demand for efficient, natural and safe skin care ingredients.