41 results about "Elastase" patented technology

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The present application relates to a kind of composition with whitening and anti-aging and its application, belong to the field of cosmetics.The composition with whitening and anti-aging of the present application includes the following mass parts of components: tetrahydro magnolia alcohol: 0.1-10 parts;Phytol: 1-5 parts;Resveratrol: 0.1-5 parts.The composition with whitening and anti-aging of the present application has significant inhibitory effect on tyrosinase and elastase, and has excellent whitening, anti-aging efficacy.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

A cosmetic composition is provided, which comprises a carrier and a Patchouli stem extract, in particular an extract from exhausted Patchouli stems. This composition provides anti-oxidant, anti-elastase, anti-hyaluronase, and anti-tyrosinase activities.

The invention relates to the technical field of cosmetics, in particular to a tightening and brightening composition containing a white truffle extract and application of the tightening and brightening composition. The composition comprises a white truffle fungus extract, a dendrobium devonianum stem extract, a spirulina maxima extract and a pyracantha fortuneana fruit extract. The composition obtained by compounding the raw materials can significantly inhibit the activity of elastase and collagenase, has a certain whitening effect, and is mild and non-irritating.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

This application relates to a species of Pseudomonas aeruginosa. bpsR Genes, BpsR proteins and their applications, engineered bacteria, and drugs, among which bpsR The nucleotide sequence of the gene is shown in SEQ ID No. 1. This invention... bpsR Genes, through regulation in Pseudomonas aeruginosa bpsR Gene expression can regulate the virulence factors of Pseudomonas aeruginosa, such as biofilm production, pyocyanin production, motility, and elastase secretion levels. Furthermore, BpsR protein can be used as a new target for the development of drugs to prevent and treat Pseudomonas aeruginosa, which has important clinical significance for the prevention and control of Pseudomonas aeruginosa infection.

This invention relates to the field of cosmetic technology, specifically to an anti-wrinkle cream containing shea butter and its preparation method. The anti-wrinkle cream containing shea butter has the following raw material composition: shea butter, moisturizer, synthetic ester, emulsifier, plant extract, pH adjuster, thickener, and water. This invention uses a combination of shea butter and a specific synthetic ester as a moisturizing base, combined with a specific ratio of nasturtium extract and red algae extract as active ingredients. The resulting anti-wrinkle cream exhibits excellent elastase inhibitory activity and effectively combats wrinkles. Simultaneously, the use of polyglycerol-6 didecanoate and cholesterol polyether-24 as a compound emulsifier system significantly improves the absolute value of the zeta potential, resulting in high product stability and a long shelf life. The preparation method of this invention is simple and suitable for industrial production.

The application discloses a firming and anti-wrinkle cream containing a gallnut extract, and belongs to the field of cosmetics. The gallnut medicine is extracted, and glycosidase is used to hydrolyze tannic acid into gallic acid, so that the hydrolysis conversion efficiency can be controlled, the prepared gallnut extract can better inhibit elastase, promote type I collagen synthesis and reduce the secretion of MMP-1, and thus the prepared cosmetic has better firming and anti-wrinkle effects.

The invention discloses a preparation method of a phallus impudicus extract as well as a product and application of the phallus impudicus extract, and belongs to the technical field of biology. The phallus impudicus extract is prepared by taking phallus impudicus buds as a raw material and adopting a water extraction process, and the mass percentage of polysaccharide in the extract is not less than 70%. The obtained extract has the biological activity effects of resisting oxidation, resisting inflammation, resisting saccharification, inhibiting elastinase, relieving repair activity or promoting skin repair, and experiments prove that the extract is free of cytotoxicity, sensitization and phototoxicity. The phallus impudicus extract is very suitable for sensitive skin and used as a daily skin care product. Development of traditional fungus phallus impudicus is led to the field of high-tech cosmetic raw materials, a complete technical scheme and solid data support are provided for high value-added utilization of phallus impudicus, and the phallus impudicus is wide in application prospect.

The invention discloses compact anti-wrinkle elastin peptide freeze-dried powder and a preparation method thereof, and belongs to the technical field of bioactive peptides. The freeze-dried powder is prepared by taking core tubes and / or throat tubes of bred animals as raw materials through cleaning and impurity removal, crushing and homogenizing, degreasing and impurity removal, composite enzymolysis, enzymolysis inactivation, centrifugal separation, gradient filtration, concentration, sterilization and vacuum freeze drying, and has remarkable elastinase inhibitory activity and tightening and anti-wrinkle effects. The livestock and poultry slaughtering byproducts which are wide in source and low in cost are selected as raw materials, high-value utilization of the livestock and poultry slaughtering byproducts is achieved, the optimized preparation technology solves the problems that in the prior art, enzymolysis efficiency is low, product purity is low, activity is insufficient, and stability is poor, the technology is simple, large-scale production is easy, and the method can be widely applied to the fields of cosmetics, food and health care products.

This invention discloses a method for preparing anthocyanin extract from tree tomato, along with its products and applications, belonging to the field of natural plant extraction and application. The method uses tree tomato peel as raw material, employing an acidic ethanol aqueous solution containing 0.5% (w / v) trifluoroacetic acid as the extraction solvent, at a material-to-liquid ratio of 1:30 (g / mL), at a temperature of 50℃, and for 60 minutes to obtain a high-content anthocyanin extract. This tree tomato anthocyanin extract possesses excellent anti-inflammatory, antioxidant, anti-glycation, elastase-inhibiting, and whitening activities, and exhibits low cytotoxicity, no phototoxicity, and no skin sensitization. The efficient and stable anthocyanin extraction process provided by this invention has high extraction efficiency and good enrichment effect of active ingredients, meeting the requirements for subsequent industrial production. The obtained extract has whitening, anti-aging, and anti-inflammatory effects, providing a high-quality raw material and formulation solution for developing multifunctional and highly safe natural plant-based skincare products.

The invention belongs to the technical field of daily skin care products, and relates to a biological agent with a bionic ECM structure and application of the biological agent in the field of skin aging resistance. The biological agent with a bionic ECM structure is prepared by simulating components of natural ECM of skin and mainly comprises fibronectin rh FN-LN, collagen, glycoprotein, serum albumin, a polyamino acid polysaccharide condensation compound, mannitol, trehalose and auxiliary materials. The auxiliary materials comprise elastin, decapeptide-4 and the like. An elastinase inhibition ratio test and a human skin fibroblast proliferation test prove that the biological agent with the bionic ECM structure has the effects of repairing, resisting aging and improving skin compactness.

The invention relates to application of an Arteridin gene as a biomarker in preparation of a product for auxiliary diagnosis of abdominal aortic aneurysm, clinical specimens and animal experiments find that the expression of Arteridin in occurrence of abdominal aortic aneurysm is remarkably increased, which indicates that Arteridin can be used as the biomarker for auxiliary diagnosis of abdominal aortic aneurysm; the invention also relates to a product for down-regulating the expression of Arterin and an application of the product in preparation of drugs for treating abdominal aortic aneurysm, the product comprises siRNA and shRNA of a targeted Arterin gene and AAV for coding the sequence of the shRNA, and after specific knockdown of Arterin by smooth muscle cells is realized, in an Elatase-induced abdominal aortic mouse model, the expression of Arterin is reduced, and the expression of Arterin is reduced. The diameter of the aneurysm is reduced, the occurrence rate is reduced, the elastin destruction degree is reduced, the expression quantity of vascular remodeling genes MMP2 and MMP9 is reduced, and the difference is obvious compared with that of a control group; the result shows that Arteridin is expected to become a target spot for treating abdominal aortic aneurysm.

The present application belongs to the field of microbial technology, and more specifically, the present application relates to an inactivated culture of plant lactobacillus BGI-N6, its use and products containing the inactivated culture. The present application provides an inactivated culture product of probiotics, which can break through the condition limitation of application of active probiotics, effectively eliminate free radicals, inhibit collagenase and elastase, inhibit skin pathogenic bacteria, so as to achieve better effect of slowing down skin aging.

This invention belongs to the field of cosmetic technology, specifically relating to a method for preparing a calcium-containing fermented lysate with anti-aging effects and its application. This invention is the first to prepare a fermented lysate with high calcium content. Experiments have shown that this calcium-containing fermented lysate can significantly promote the relative gene expression levels of collagen and elastin, exhibiting significant anti-wrinkle effects. Simultaneously, this calcium-containing fermented lysate can effectively inhibit the activity of elastase, resulting in a significant firming effect. Furthermore, this invention applies the calcium-containing fermented lysate to cosmetics. The interaction of the components in this calcium-containing fermented lysate composition not only improves the skin's absorption rate but also effectively enhances the stability of the composition, thus facilitating the stable exertion of its anti-aging effects. This makes it a relatively ideal anti-aging cosmetic.

The invention belongs to the technical field of biochemistry, and relates to a male camel semen liquefying agent as well as a preparation method and application thereof. The invention provides a male camel semen liquefying agent. The male camel semen liquefying agent comprises a proteolysis agent and an auxiliary agent, the proteolysis agent is elastase, papain, streptavidin and superoxide dismutase; the auxiliaries are vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid)-Na, MgCl2 and a solvent. The male camel semen liquefying agent provided by the invention can effectively promote the liquefaction of male camel semen and maintain the sperm motility after the liquefaction of the male camel semen. The technical problem that conventional protease is not suitable for male camel semen liquefaction is solved.

The application belongs to the technical field of cosmetic production and discloses an anti-wrinkle firming composition and purposes and cosmetics thereof, which comprise oregano leaf extract and tribulus terrestris fruit extract, and the mass ratio of the oregano leaf extract to the tribulus terrestris fruit extract is 1-10:5-15. The application can effectively provide good anti-wrinkle firming effect for the skin in the aspects of free radical scavenging and elastase inhibition capacity improvement through the synergistic effect between the oregano leaf extract and the tribulus terrestris fruit extract. In addition, the anti-wrinkle firming capacity of the composition can be further improved after adding yarrow extract to the composition.

This invention relates to the field of in vitro diagnostics, and particularly to a combined diagnostic kit for prostate diseases. The invention provides reagents including pH, peroxidase, elastase, lecithin bodies, citric acid, zinc, and acid phosphatase under mild reaction conditions, enabling rapid differentiation of inflammatory and non-inflammatory prostatitis, benign prostatic hyperplasia, and non-prostatic genital tract infections using a single test card. Through extensive experimentation, this invention has developed a one-step reaction scheme suitable for the dry chemical determination of acid phosphatase and lecithin bodies in seminal plasma. This method eliminates the need for a stop solution, achieving a one-step reaction under mild conditions that will not cause damage to operators or fully automated instruments. The samples used are seminal plasma or semen, allowing for large sample volumes and easy acquisition, significantly improving diagnostic efficiency for doctors and patient comfort.

The application discloses a firming and anti-wrinkle emulsion containing a gallnut extract and belongs to the field of cosmetics. The gallnut medicinal material is extracted, and glycosidase is used to hydrolyze tannic acid into gallic acid, so that the hydrolysis conversion efficiency can be controlled, the prepared gallnut extract can better inhibit elastase, promote the synthesis of type I collagen and reduce the secretion of MMP-1, and thus the prepared cosmetic has better firming and anti-wrinkle effects.

This invention discloses a strain of *Camellia sinensis* derived from the thousand-year-old red camellia and its application in the preparation of anti-aging and repairing active oils. The *Camellia sinensis* strain, named *Camellia sinensis 1021*, is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 39106. This invention also provides a specific screening method for this strain and a method for preparing anti-aging and repairing active oils using this strain through fermentation. The oil is orange-red in color, contains a high proportion of diglycerides and monoglycerides, and exhibits excellent in vitro antioxidant activity (high scavenging rate of DPPH and ABTS free radicals), as well as anti-aging and repairing effects such as promoting cell migration and I-collagen synthesis and inhibiting elastase. The strain of this invention is highly targeted, the fermented oil has high activity, and the process is mild and easily scalable. The product can be widely used in cosmetics, food additives, or health products.

The present invention relates to granulocyte precursor cell that has been differentiated in vitro, wherein the granulocyte precursor cell comprises: (a) increased expression of one or more of: serglycin (SRGN), myeloperoxidase (MPO), major histocompatibility complex, class II, DR alpha (HLA-DRA), CD74, and elastase (ELANE) when compared to an equivalent granulocyte precursor cell that has been differentiated in vivo; and / or (b) decreased expression of one or more of: defensin alpha 1 (DEFA1), defensin alpha 3 (DEFA3), cathelicidin antimicrobial peptide (CAMP), bactericidal permeability increasing protein (BPI), and azurocidin 1 (AZU1) when compared to an equivalent granulocyte precursor cell that has been differentiated in vivo. Also provided are cells, methods for producing the same, uses of the same, and kits comprising the same.

This invention proposes a method for preparing a single-cell suspension of porcine pancreatic cells and its application. The method includes the following steps: S1, taking porcine pancreatic tissue, removing surface fat, and mincing the tissue; S2, washing the tissue fragments, and then placing the tissue into a mixed enzyme dissociation solution for the first digestion; S3, after the first digestion, collecting the supernatant, and adding the remaining tissue to the mixed enzyme dissociation solution for a second digestion; S4, after the second digestion, collecting the supernatant and combining it with the supernatant from step S3, and filtering; S5, centrifuging the filtered dissociation solution, discarding the supernatant, and resuspending the cell pellet to obtain a cell suspension; the mixed enzyme dissociation solution includes trypsin, collagenase I, elastase, lipase, DNase I, heparin, sodium sulfate, and DH Kans buffer. The enzyme mixture of this invention has the effects of removing intercellular matrix, reducing cell aggregation, protecting cell membrane morphology, and reducing cell damage.

The invention relates to a whitening and anti-aging composition and application thereof, and belongs to the technical field of cosmetics. The whitening and anti-aging composition provided by the invention comprises the following components in parts by mass: 0.1-10 parts of tetrahydromagnolol; 1 to 5 parts of phytol; and 0.1 to 5 parts of resveratrol. The whitening and anti-aging composition disclosed by the invention has a remarkable inhibition effect on tyrosinase and elastase, and has excellent whitening and anti-aging effects.

This invention discloses the application of glycyrrhizin in the preparation of cosmetics and its cosmetic compositions, belonging to the field of traditional Chinese medicine research and development. Glycyrrhizin is derived from licorice extract purified from licorice raw materials, with a purity of ≥98%. Based on its antioxidant, anti-inflammatory, anti-glycation, elastase inhibitory, and cell migration and repair promoting functions, glycyrrhizin can be used to prepare anti-aging or soothing repair cosmetics. Furthermore, within its effective concentration range, it exhibits no cytotoxicity, non-sensitization, or phototoxicity, making it suitable for preparing anti-aging or soothing repair cosmetic compositions. This cosmetic composition has been proven to significantly improve skin elasticity, reduce stinging and redness, and possesses anti-aging and skin-soothing repair effects, making it widely applicable for the daily maintenance and stabilization of sensitive skin. This invention fills a gap in the application of glycyrrhizin in the cosmetic field, providing favorable support for the widespread application of licorice in cosmetics.

The invention relates to an anti-aging composition containing recombinant humanized collagen as well as a preparation method and application of the anti-aging composition, and the anti-aging composition comprises the following components in parts by weight: 1-4 parts of recombinant humanized III-type collagen, 7-10 parts of a rhodiola rosea extract, 3-4 parts of a malachite extract and 6-11 parts of a spanishneedles herb extract. The prepared anti-aging composition can play a role in delaying skin aging through the ways of inhibiting the activity of collagenase and elastase, neutralizing free radicals, reducing oxidative damage, adjusting transportation of moisture and glycerol, maintaining the hydrated state of skin and the like.

The invention discloses an optical fiber biosensor, which is prepared by coating the surface of an amino-functionalized waist-tapered optical fiber sensor with a graphene oxide layer and adsorbing an aptamer NE-AP or an aptamer LF-AP on the surface of the graphene oxide layer. The aptamer NE-AP can be specifically combined with the elastinase ELANE; the aptamer LF-AP can be specifically combined with the lactoferrin LF. After the optical fiber biosensor captures ELANE and LF proteins, the roughness and thickness of the surface of the optical fiber at the waist-cone-shaped part are changed, when light penetrates through the waist-cone-shaped part covered by biomolecules, the effective refractive index of the surface of the optical fiber biosensor is changed due to the fact that the diameter is gradually reduced and an evanescent field outside a core is enhanced, and the optical fiber biosensor can be used for detecting the optical fiber in the waist-cone-shaped part. Therefore, red shift occurs according to a transmitted spectrum induced after aptamer-protein combination is tracked, and high-sensitivity and low-detection-limit detection on the activity of the neutrophil is realized. The method has the characteristics of high sensitivity and low detection limit.