50 results about "Broussonetia" patented technology

The invention provides a paper mulberry fruit natural product efficient preparation method based on ultrasonic extraction, which comprises the following steps: S1, raw material pretreatment: crushing dried paper mulberry fruits, and sieving with a 40-60-mesh sieve to obtain paper mulberry fruit powder; s2, composite enzymatic hydrolysis pretreatment: adding deionized water of which the mass is 0.5-1.5 times of that of the fructus broussonetiae powder into the fructus broussonetiae powder in the S1, uniformly stirring, then adding a compound enzyme of which the mass is 0.1-0.5% of that of the powder, and carrying out enzymatic hydrolysis for 30-90 minutes under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-5.5; the compound enzyme is formed by compounding pectinase and cellulase according to the mass ratio of 1: 1-3: 1, the pectinase and the cellulase are subjected to compounding pretreatment, impurity structures are accurately disintegrated, dissolution of pectin and polysaccharide is reduced from the source, a single-frequency mode is abandoned in an ultrasonic link, and flavone is protected through low-frequency strong cavitation wall breaking, high-frequency mild mass transfer and pulse temperature control. The two methods are not simply superposed, enzymolysis is a foundation for ultrasonic directional extraction, ultrasonic amplification of the enzymolysis effect finally solves the traditional problems of many impurities and low efficiency, and a green and efficient flavone extraction scheme is formed.

The invention provides an application of cudrania tricuspidata polysaccharide in preparation of a medicine for treating or preventing HCMV infection, the cudrania tricuspidata polysaccharide ZGs-1 shows a remarkable inhibition effect on expression of immediate early protein IE1 / 2 and early protein p52 and copy numbers of virus genes UL123, UL44 and UL32 in a plurality of key links of HCMV infection, and has definite antiviral activity. Due to the natural source characteristic of the cudrania tricuspidata polysaccharide ZGs-1, the cudrania tricuspidata polysaccharide ZGs-1 is high in safety and low in cytotoxicity, and the cudrania tricuspidata raw material is easy to obtain, so that a candidate drug or functional component with remarkable potential is provided for preventing and treating the HCMV, and the cudrania tricuspidata polysaccharide ZGs-1 has a good development prospect and application value.

This invention discloses a kidney-tonifying and blood-activating traditional Chinese medicine composition and its application in improving postoperative visual function, belonging to the field of pharmaceutical technology. The kidney-tonifying and blood-activating traditional Chinese medicine composition of this invention is made from 10 parts of Cuscuta chinensis, 15 parts of Broussonetia papyrifera fruit, 18 parts of Lycium barbarum, 15 parts of Leonurus japonicus, 20 parts of Leonurus japonicus, 10 parts of Ligusticum chuanxiong, 9 parts of Prunus persica kernel, 6 parts of Carthamus tinctorius, 15 parts of Poria cocos, 18 parts of Albizia julibrissin bark, 12 parts of Astragalus membranaceus, and 12 parts of Pueraria lobata root. It can be standardized and adjusted according to clinical complications. This invention focuses on the critical point of "kidney deficiency and blood stasis" one month after posterior scleral reinforcement surgery, using the principles of tonifying the liver and kidneys and activating blood circulation to improve ocular microcirculation, enhance and stabilize vision, thereby protecting visual function, and conforming to the treatment principle of "treating the root cause when the condition is mild."

This invention discloses a traditional Chinese medicine composition for treating degenerative bone diseases, its preparation method, and its application. It is made from the following raw materials in parts by weight: 30-50 parts Rehmannia glutinosa (processed), 15-30 parts deer antler glue, 20-80 parts Cistanche deserticola, 15-50 parts Morinda officinalis, 15-50 parts tortoise shell glue, 30-80 parts Lycium barbarum, 10-50 parts Curculigo orchioides, 15-50 parts Eucommia ulmoides, 20-80 parts Cuscuta chinensis, and 30-50 parts Dioscorea opposita. 0 parts, Drynaria fortunei 15-50 parts, Achyranthes bidentata 10-50 parts, Taxillus chinensis 20-50 parts, Broussonetia papyrifera 15-30 parts, Clematis chinensis 15-50 parts, Acanthopanax senticosus 15-50 parts; Through the formulation strategy of "regulating the liver and kidneys, nourishing essence and blood, and combining tonification and purging", combined with a specific extraction process, it systematically regulates the balance of bone metabolism, achieves dual prevention and treatment of osteoporosis and bone hyperplasia, and treats both the symptoms and the root cause, and has high safety for long-term use.

The application discloses a broussonetia papyrifera type fermented feed and a preparation method thereof, and belongs to the field of feed preparation, and comprises fermented broussonetia papyrifera, corn starch, soybean meal, puffed soybean powder, sodium chloride, calcium hydrogen phosphate, an absorption promoting agent and a premix ingredient, wherein the fermented broussonetia papyrifera is obtained by fermentation treatment of the broussonetia papyrifera by a composite microbial agent. The broussonetia papyrifera is high in crude protein content after the fermentation treatment, and is also rich in nutritional ingredients such as vitamins, carbohydrates and trace elements; the use of the composite microbial agent can help improve the fermentation effect of the broussonetia papyrifera, effectively degrade biological macromolecules such as crude fiber and improve the digestion and absorption rate of nutritional substances; in the preparation process of the feed, after the static treatment, secondary fermentation is equivalent to be carried out, the active peptides and the active polysaccharides in the feed can be improved, and a large number of beneficial active bacteria are retained, so that the feed is not only ensured to be balanced in nutrition, but also is ensured to have a unique flavor, and the feed taste is helped to be improved.

The present application belongs to the field of bioengineering technology, and particularly relates to a kind of enzymatic broussonetia papyrifera powder and its preparation method and application.The present application uses Aspergillus niger WZ001 as a starting strain, constructs and selects a strain of high-yield Aspergillus niger tannase, and then uses the recombinant tannase obtained by fermentation to enzymatically hydrolyze tannin in broussonetia papyrifera powder, which can convert broussonetia papyrifera powder tannin into gallic acid, which can effectively improve the palatability of feed using broussonetia papyrifera powder as one of the raw materials.On this basis, since the Aspergillus niger host has a rich enzyme system, amylase, glucoamylase, protease, cellulase, etc., the Aspergillus niger tannase of the present application can play the role of a complex enzyme, so it can enzymatically hydrolyze broussonetia papyrifera powder more comprehensively, and the product obtained after enzymatic hydrolysis has a higher nutritional value, which reflects a high economic value and social benefit.In addition, the Aspergillus niger tannase constructed in the present application can meet the requirements of food-grade enzyme preparation, so the safety of the enzymatically hydrolyzed broussonetia papyrifera powder is guaranteed.

The invention relates to the field of termite monitoring, in particular to bait for a termite monitoring device and a preparation method and application of the bait. The bait comprises broussonetia papyrifera wood powder, a binder, a preservative and pheromone microcapsules, the addition amount of pheromone in the bait is 0.01-0.1 mg / Kg, and the mass ratio of the broussonetia papyrifera wood powder to the binder to the preservative is (90-95): (0.5-5): (0.1-0.2). The bait capable of being used for adding pheromones to the termite monitoring device is provided, the attractive force of the termite monitoring device to termites is enhanced, and the termite monitoring efficiency is improved.

The invention discloses a traditional Chinese medicine medicated bath medicine bag for treating wind-cold-dampness arthralgia, and belongs to the technical field of traditional Chinese medicines. The medicine bag is prepared from 10-20 parts by mass of broussonetia papyrifera roots, pine roots, albizia roots, Chinese redbud roots, maidenhair roots, mulberry roots, banyan roots, camphor tree roots, paulownia roots and willow roots, preferably 15 parts. The preparation method comprises the steps of raw material weighing, drying treatment, mixing and subpackaging into an air-permeable fiber medicine bag, the medicine bag is a 20 cm * 15 cm rectangular cloth bag, three sides are sealed, and one side is provided with an opening and closing structure. Broussonetia papyrifera roots and camphor tree roots are used as monarch drugs together, and synergistically expel wind, remove cold, remove dampness and activate blood, and aim at the core pathogenesis of wind-cold-dampness arthralgia; the root medicinal materials of the whole formula walk through lower jiao and directly reach muscles and bones, are externally applied through fumigation medicated bath, avoid burdens of gastrointestinal tracts, livers and kidneys caused by oral administration, have the advantages of strengthening body resistance and harmonizing, quick response, direct action, high safety and the like, are convenient to use, and are suitable for family health care and clinical popularization.

The application discloses fermented broussonetia papyrifera feed for improving growth performance and meat quality of Taihe black-bone chickens and application thereof. The feed is prepared through the following steps: (1) preparation of fermented broussonetia papyrifera, which is prepared by fermenting broussonetia papyrifera, lactic acid bacteria and yeast at room temperature for 21 days; (2) customization of fermented broussonetia papyrifera feed formula, wherein rice bran in a daily ration of Taihe black-bone chickens in a growing period is replaced by an equal amount of fermented broussonetia papyrifera, and the replacement amount accounts for 4% of the total weight of the daily ration; and (3) preparation of fermented broussonetia papyrifera feed. Compared with a control group, when the fermented broussonetia papyrifera accounts for 4% of the total weight of the daily ration, the growth performance, apparent metabolic rate of nutrients and meat quality of Taihe black-bone chickens in the growing period can be effectively improved.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

The invention relates to a broussonetia papyrifera leaf quality evaluation method, equipment and a medium. The method comprises the following steps: firstly, obtaining broussonetia papyrifera leaves from different producing areas, performing ultrasonic extraction on dried, crushed and sieved broussonetia papyrifera leaf powder with methanol, and preparing a test solution and a reference solution; secondly, carrying out sample injection detection on the solution by adopting a high performance liquid chromatography method, introducing a map into a similarity evaluation system, and establishing a characteristic map by taking the broussonetia papyrifera leaf map as a reference; based on a UPLC-QE-HRMS / MS technology, carrying out primary and secondary mass spectrometry analysis on common peaks in the characteristic chromatogram, and comparing and analyzing chemical components of the characteristic peaks in combination with a database; finally, the analyzed characteristic flavonoids are used as internal standard substances, the relative content of characteristic peak chemical components is quantitatively analyzed, and a quality evaluation result is obtained by determining the lower limit of the content. By adopting the method, comprehensive characterization of the chemical components of the paper mulberry leaves can be realized, the limitation of single component detection in the prior art is effectively solved, and a systematic scheme is provided for quality consistency evaluation of the paper mulberry leaves from different producing areas.

The invention relates to the field of livestock and poultry breeding, and discloses a laying hen feed additive based on fermented broussonetia papyrifera and application of the laying hen feed additive. The additive is prepared by taking paper mulberry leaves as a main material, matching with rice husks, wheat bran and other auxiliary materials, inoculating a microecological preparation containing lactobacillus plantarum, candida utilis and bacillus subtilis, and performing anaerobic fermentation. 4% of the additive is used for daily ration of the aged laying hens, so that the laying rate can be remarkably increased, the dirty egg rate can be reduced, and the egg weight, the eggshell strength and the yolk color can be improved; the mechanism is to improve the form of the intestinal tract, enhance the expression of tight junction protein, regulate immune balance, optimize the structure of cecum flora and enrich beneficial microorganisms; the broussonetia papyrifera leaf feed additive realizes efficient utilization of broussonetia papyrifera leaf resources, provides a safe and effective functional additive for prolonging the laying period of laying hens and improving the breeding benefits, and has a good application prospect.

The application discloses a gene BpMIXTA01 for regulating and controlling initial development of Broussonetia papyrifera epidermal hair as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2. The gene belongs to the ninth sub-group MIXTA gene of the R2R3 MYB transcription factor family. Function verification shows that overexpression of BpMIXTA01 in Arabidopsis thaliana can significantly increase the number of epidermal hairs, and can restore the epidermal hair loss phenotype of the Arabidopsis thaliana hairless mutant, proving that BpMIXTA01 is a positive regulation factor of epidermal hair formation. The application first discloses a key regulation gene for initial development of Broussonetia papyrifera epidermal hair cells, provides an important gene resource for analyzing the molecular mechanism of epidermal hair development of woody plants, and can cultivate a hairless forage Broussonetia papyrifera new variety through genetic engineering means, and has important theoretical value and application prospect.

The utility model discloses an air-drying machine for paper mulberry processing, which belongs to the technical field of air-drying of paper mulberry and comprises a base, an air-drying rack slidably arranged on one side of the top surface of the base and an auxiliary air-drying mechanism arranged on the other side of the top surface of the base, the auxiliary air-drying mechanism comprises a cover, one side of the cover is open, and the cover is fixed on the top surface of the base. An opening of the cover faces the airing frame, a heating plate is arranged on the inner wall of the cover, a material taking assembly is installed at the top of the cover, and the airing frame comprises two installation columns arranged on the top face of the base in a sliding mode and an airing base installed between the two installation columns. When the broussonetia papyrifera is used for airing, the broussonetia papyrifera is naturally aired on the inclined surface of the airing seat, when the weather is not suitable for airing, the broussonetia papyrifera is placed on the inclined surface of the airing seat, then the airing seat is pushed into the cover, and the heating plate on the inner wall of the cover is started to dry the broussonetia papyrifera, so that the broussonetia papyrifera can be manufactured in different natural environments, and the manufacturing efficiency of the broussonetia papyrifera is effectively improved.

The invention discloses a broussonetia papyrifera ecological fermentation additive feed and a preparation method thereof, and belongs to the technical field of feed preparation. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 150 to 250 g of leaves of broussonetia papyrifera, 10 to 15 g of probiotics, 10 to 15 g of hawthorn, 15 to 20 g of pericarpium citri reticulatae, 15 to 25 g of liquorice root, 3 to 6 g of cortex cinnamomi, 10 to 15 g of radix astragali seu hedysari, 10 to 15 g of rhizoma atractylodis macrocephalae, 15 to 20 g of pericarpium granati, 5 to 10 g of spica prunellae, 5 to 10 g of rhizoma belamcandae, 5 to 10 g of radix salviae miltiorrhizae, 10 to 15 g of endothelium corneum gigeriae galli, 20 to 25 g of honeysuckle flower, 15 to 20 g of radix According to the invention, the broussonetia papyrifera leaves are adopted as a main raw material and are combined with other auxiliary components, so that nutritional ingredients such as high protein, vitamins and minerals in the broussonetia papyrifera leaves can be fully utilized, and the feed with balanced nutrition is provided for livestock and poultry, so that the growth speed and the production efficiency of animals can be improved, and the health condition of the animals can be improved; and secondly, the probiotics are added in an ecological fermentation manner, so that the microbial environment of the feed can be effectively improved, and the growth of the beneficial bacteria is promoted.

The invention relates to a hybrid broussonetia papyrifera leaf induced plant regeneration and vitrification clumpy bud rejuvenation method. The method comprises explant sterilization, budding induction, rooting induction and vitrification clumpy bud rejuvenation. The leaves are subjected to gradient sterilization and inoculated to the same culture medium, callus induction, budding induction and proliferation can be conducted at the same time, high buds can be directly cut for rooting induction without strong seedling culture after budding, and vitrified clumpy buds appearing in the culture process can be recovered to be robust. The method is rich in material source and simple in culture process, avoids material pollution caused by frequent subculture and updating of a turnover culture medium, saves labor cost, ensures that the callus induction rate and the budding induction rate are up to 100%, the budding number of a single callus is up to 12.7-19.2, the plant regeneration time is short, vitrified bud seedlings can be fully utilized, material waste is avoided, and the method is suitable for industrial production. The breeding efficiency is improved, the seedling production cost is reduced, and industrialization is facilitated.

The present application relates to the technical field of biology, and particularly relates to a broussonetia papyrifera pollen allergen extract, a preparation method and application thereof.The present application provides a broussonetia papyrifera pollen allergen extract, which comprises a peptide segment composition; and the amino acid sequence of the peptide segment composition is shown as SEQ ID NO:1 to SEQ ID NO:104.The present application provides a commercialized broussonetia papyrifera allergy diagnosis reagent, which can meet the diagnosis and treatment requirements of broussonetia papyrifera pollen allergy patients in the clinic.

The application provides a traditional Chinese medicine preparation for treating diabetic nephropathy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine pharmacy. The traditional Chinese medicine preparation is prepared from traditional Chinese medicinal materials such as angelica, eucommia ulmoides, helicteres angustifolia, broussonetia papyrifera, radix paeoniae suffruticosae, curcuma longa, dendrobium, polygonatum, radix adnati, ligustrum lucidum, ipomoea purpurea, lagerstroemia indica, kochia scoparia, haes, pyrrosia lingua and herba barbatae, and has the effects of nourishing yin and kidney, promoting blood circulation to remove meridian obstruction and clearing heat and removing dampness. The traditional Chinese medicine preparation can up-regulate the expression levels of podocin and nephrin, inhibit inflammatory mediators, improve the blood sugar, urine protein, kidney function and kidney pathological injury of DN rats, provides a new idea for the treatment of diabetic nephropathy, and has a good development and application prospect.

The invention discloses a traditional Chinese medicine ointment for improving pancreas islet function and a preparation method and application thereof. The traditional Chinese medicine ointment is prepared from ginseng, astragalus membranaceus, radix ophiopogonis, salvia miltiorrhiza, corn stigma, radix polygonati officinalis, radices trichosanthis, dandelion root, Chinese yam, poria cocos, radix puerariae, purslane, potentilla discolor, cyclocarya paliurus, folium mori, liquorice, momordica grosvenori, radix rehmanniae recen, coptis chinensis, chrysanthemum, honeysuckle, buckwheat, rhizoma anemarrhenae, cinnamon, bitter gourd, gynostemma pentaphyllum, cortex lycii radicis, rhizoma phragmitis and the like according to a specific weight part ratio. The invention further provides a preparation method of the traditional Chinese medicine ointment. The preparation method comprises the steps of soaking in water, decocting and extracting, filtering and concentrating, ointment collecting and the like. The traditional Chinese medicine ointment can remarkably reduce fasting blood glucose and postprandial blood glucose of diabetic model mice and improve abnormal glucose tolerance, and has a protection effect on pancreas islet tissues. The traditional Chinese medicine ointment can be used for preparing health care products for assisting in reducing blood sugar or pharmaceutical compositions for preventing and assisting in treating type 2 diabetes mellitus, and has the advantages of reasonable formula, exact curative effect, stable preparation process and the like.

The present application relates to sewage treatment technical field, especially a kind of sewage and waste water reuse water treatment agent and its preparation method.It is by mass fraction, the sewage and waste water reuse water treatment agent includes following raw materials: polyaspartic acid 10-15 parts, modified bentonite 5-10 parts, sodium gluconate 3-5 parts, ethylenediaminetetraacetic acid tetrasodium 5-8 parts, broussonetia papyrifera leaf extract 1-3 parts, water 45-55 parts.The treatment agent has excellent scale inhibition, bacteriostasis, heavy metal removal and organic pollutant removal performance, and environment-friendly, no secondary pollution.

The application discloses a kind of broussonetia papyrifera characteristic map and the construction method of contrast map and the method for distinguishing broussonetia papyrifera genuine fake.The broussonetia papyrifera test sample solution, reference substance solution is injected into ultra-high performance liquid chromatography respectively, and characteristic map is obtained;Characteristic map is introduced into traditional Chinese medicine chromatographic fingerprint similarity evaluation system to establish contrast map.The characteristic peak is compared with the peak of reference substance solution atlas, and the test sample is distinguished as broussonetia papyrifera or fake.The characteristic map of the application is rich in chromatographic information, high in specificity, short in time, good in chromatographic peak separation degree, and stable baseline.The technology has good reproducibility and high stability;It provides technical support for the authenticity identification of broussonetia papyrifera raw materials and preparations;The method is simple, accurate and reliable, easy to operate, compared with traditional crude drug identification method, less sample amount is needed, detection time is saved, and efficiency is higher;Solvent consumption is less, and the toxicity of solvent used is smaller, and the environmental pollution is small.

The present application provides a broussonetia automatic seedling device and control method, the device contains mounting plate, pit unit, conveying unit, filling unit, control unit, irrigation unit, level, mobile wheel and image recognition unit. The pit, conveying, filling unit are all set in the mounting plate, the irrigation unit is equipped with a solenoid valve, and is connected with the control unit. The level is also connected with the control unit. The image recognition unit is integrated in the control unit, which identifies the position of the tree planting pit and controls the conveying unit to accurately send seedlings. The present application uses the level to detect the state of the device in real time. When the inclination angle of the device is greater than the preset threshold, the level sends a signal to the control unit. After receiving the signal, the control unit controls the telescopic rod to adjust the device, so that the device returns to the horizontal state. At the same time, the device makes the broussonetia always perpendicular to the horizontal plane when planting, preventing the broussonetia from growing obliquely, thereby improving the growth quality and survival rate of the broussonetia.

The invention belongs to the technical field of genetic engineering and plant genetic improvement, and particularly relates to a paper mulberry sulfate transporter gene BpSULTR3. 1 and application thereof. The invention provides a preparation method of broussonetia papyrifera BpSULTR3. The invention also provides application of the gene sequence in enhancing the selenium accumulation capacity of plants and promoting the synthesis of organic selenium of the plants. The gene is proved to be a key gene for regulating selenium absorption and metabolism of the broussonetia papyrifera, so that the blank of research on a selenium enrichment molecular mechanism of the broussonetia papyrifera is filled; the BpSULTR3 is subjected to overexpression; the total selenium content of plants can be increased by more than two times, and the proportion of organic selenium is remarkably increased; through selenium form and content determination and quantitative analysis of related enzyme genes in a selenium metabolic pathway, BpSULTR3 is disclosed; 1 participates in a molecular mechanism of selenate absorption, organic selenium synthesis, especially selenomethionine synthesis. The method is suitable for selenium-enriched feed, selenium-enriched agricultural product development and selenium-polluted soil treatment, and both economic value and ecological value are considered.

The application belongs to the technical field of biological medicine, and particularly relates to a screening and preparation method of Broussonetia papyrifera branch extract with anti-inflammatory and antioxidant activities and application thereof. The anti-inflammatory and antioxidant active sites in the Broussonetia papyrifera branch are screened and separated based on the activity-oriented / spectrum-effect separation technology for the first time. The technical scheme effectively simplifies the steps of separation operation, reduces the loss of compounds in separation, and reduces the consumption of separation mobile phase and filler. At the same time, the activity-oriented separation can improve the probability of discovering active compounds, reduce the cost of determining the activity of obtained compounds, and has good practical application value.

The invention provides a polypeptide medicine for treating osteoporosis and a preparation method of the polypeptide medicine, and belongs to the technical field of biological medicine, and the polypeptide medicine comprises the following components: Pleurotus niger peptide, soybean meal protein peptide, a Chinese sumac leaf-broussonetia papyrifera leaf extract, a ulmus pumila leaf-Sophora japonica leaf extract and the like. The method comprises the following steps: sequentially fermenting Pleurotus tuber-regium through candida utilis and lactobacillus plantarum, carrying out enzymolysis through ficin protease, and purifying through a G-50 sephadex column to obtain the Pleurotus tuber-regium peptide. The soybean meal protein peptide is prepared by sequentially carrying out enzymolysis on soybean meal through cellulase, trypsin and bromelain and purifying through an LH-60 sephadex column. The Chinese sumac leaves and the paper mulberry leaves are extracted through an ethanol aqueous solution and purified through a DEAE cellulose column, and the Chinese sumac leaf-paper mulberry leaf extract is prepared. The ulmus pumila leaves and the sophora japonica leaves are sequentially subjected to enzymolysis through cellulase, pepsin and ficin, and purification is conducted through a 732 cation exchange resin column to prepare the ulmus pumila leaf-sophora japonica leaf extract. The components are compounded, so that the bone resorption process is blocked, bone formation and mineralization are strengthened, and osteoporosis is improved.

This invention discloses a compound plant growth regulator, its preparation method, and its application, belonging to the technical field of plant growth regulators. The compound plant growth regulator of this invention is prepared from the following raw materials: *Broussonetia papyrifera* leaf extract, saikosaponin A, calcium cyclohexane, tebuconazole, aminoethyl ester, 24-epibrassinolide, fulvic acid, surfactant, and deionized water. This plant growth regulator significantly improves the lodging resistance of soybeans, optimizes yield composition, and increases yield through the synergistic effect of specific components. The innovatively added *Broussonetia papyrifera* leaf extract and saikosaponin A can both reduce plant height, increase stem diameter, and simultaneously improve seed setting rate and 100-seed weight. Furthermore, the formulation of this invention uses environmentally friendly surfactants and homogenization processes, resulting in good dispersibility and storage stability, meeting the needs of green and efficient agriculture and possessing significant agronomic promotion value.

The application discloses a non-grain raw material processing method and application of a product thereof in pig feed, and belongs to the technical field of feed processing. The processing method takes leaves of broussonetia papyrifera, citrus peel residues and perilla frutescens meal as raw materials, carries out mixing and crushing, adds water to prepare a slurry, adds calcium dihydrogen phosphate and citrate to control pH, carries out in-situ mineralization to make amorphous calcium phosphate and active components co-precipitate and assemble, and then carries out low-temperature drying to obtain composite particles; the product can be applied to full non-grain pig feed as a core functional component, and is suitable for different formulae of piglets, growing pigs and pregnant sows at different stages. The application discloses a trinity composite structure constructed by taking plant cell walls as a natural template, and solves the problems of easy inactivation and low bioavailability of active components of non-grain raw materials, realizes precise delivery of stomach protection-small intestine release-large intestine regulation, significantly improves pig growth performance, immunization level and reproduction efficiency, and provides an efficient technical scheme for industrialization of full non-grain feed.

The invention belongs to the technical field of water treatment, and particularly relates to a cyanobacterial bloom inhibitor and a preparation method thereof. The cyanobacterial bloom inhibitor is prepared from the following raw materials in parts by weight: 10 to 20 parts of sheareria nana extract, 3 to 5 parts of broussonetia papyrifera bark extract and 3 to 9 parts of fructus gardeniae extract. The novel cyanobacterial bloom inhibitor is developed, the photosynthesis capacity of cyanobacteria is reduced, the cell structure of cyanobacteria is destroyed, and the cyanobacterial bloom inhibitor has wide application prospects in the aspect of cyanobacterial bloom control. Moreover, the plant extract is adopted as the cyanobacterial bloom inhibitor, has the advantages of low cost, multiple types, high safety and the like, and can meet the requirements of different water areas and pollution degrees.

The application provides raw Xuan paper and a preparation method thereof. The raw Xuan paper is prepared by using broussonetia papyrifera pulp and reed canary grass pulp as main materials, and adding a small amount of artemisia argyi pulp, a small amount of aerogel silicon dioxide and actinidia kiwi juice, and further spraying a cationic quaternary ammonium cellulose material on the surface of the paper. The raw Xuan paper prepared by the application has excellent insect resistance and bacterial corrosion resistance, and good oxidation resistance, so that the paper can be stored for a long time and is not easy to discolor. Meanwhile, the paper has small deformation and good shape stability after being soaked in water, and is not easy to stretch and deform. The raw Xuan paper prepared by the application can be used for calligraphy, painting, stele rubbing and mounting, and meets the requirements of good ink wetting performance, uniform ink diffusion, clear ink color gradation and clear level, good toughness, folding resistance and tensile resistance, and the like. Moreover, the preparation method is simple, the conditions are mild, the raw materials are abundant and easy to obtain, and the application is more conducive to industrialized production and popularization and application.

The invention relates to the technical field of biology, in particular to a paper mulberry pollen allergen extract as well as a preparation method and application thereof. The invention provides a broussonetia papyrifera pollen allergen extract. The broussonetia papyrifera pollen allergen extract comprises a peptide fragment composition, the amino acid sequences of the peptide fragment composition are as shown in SEQ ID NO: 1 to SEQ ID NO: 104. The invention provides a commercial broussonetia papyrifera allergy diagnostic reagent which can meet clinical diagnosis and treatment requirements of broussonetia papyrifera pollen allergy patients.