33results about How to "High induction rate" patented technology

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. The invention provides a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. Kinetin KT (Kinetin) in the tissue culture and rapid propagation culture medium provided by the invention can promote the generation of buds and the growth of adventitious buds. 6-BA is 6-benayl aminopurine and is used for promoting the growth of buds; tDZ is thiabendazole, and growth and development of buds are promoted. Under the combined action of KT, 6-BA and TDZ, the bud induction rate and the bud multiplication coefficient of the stem with the bud are improved. The invention further provides a tissue culture and rapid propagation method of the Shaniodendron subaequalis, stem segments subjected to induction culture are low in pollution rate, high in survival rate and low in browning rate; the lateral bud induction rate of the stem section is as high as 91%, and the multiplication coefficient, the rooting rate and the transplanting survival rate are high.

The invention relates to the field of plant tissue culture, and discloses a method for culturing tissue culture seedlings of small angel viridae, which comprises the following steps: S1, explant selection and primary culture; s2, carrying out subculture multiplication culture; s3, rooting culture; s4, domesticating and transplanting; in the primary culture stage, MS is used as a basic culture medium, a proper amount of 6-BA, NAA, sucrose and agar are added, and induction of cluster buds is promoted; in the subculture multiplication culture stage, an optimal multiplication culture medium is screened through an orthogonal test, so that the multiplication coefficient is improved; in the rooting stage, key factors such as inorganic salt concentration, NAA and banana puree in MS are optimized, and the number and quality of root systems are improved; in the domestication transplanting stage, the optimal proportion of river sand, coco coir and peat soil is researched, and the transplanting survival rate and the tissue culture seedling quality are effectively improved. By means of the method, efficient propagation of the small angel vitis vinifera and large-scale production of high-quality seedlings are achieved, and important technical support is provided for industrial popularization of the plant.

The invention relates to an anti-browning method in a coconut microspore somatic embryo culture process, and belongs to the technical field of coconut tissue culture. The anti-browning method in the coconut microspore somatic embryo culture process comprises the following steps: S1, anther collection and pretreatment; s2, anther observation, disinfection and separation; s3, anther culture and embryo culture. According to the anti-browning method in the coconut microspore somatic embryo culture process, PVP is added into the culture medium to resist the browning phenomenon in the coconut microspore embryo induction process; when the explant is cut, a citric acid solution is dipped to reduce browning caused by physical damage; ascorbic acid (vitamin C) is also added into the culture medium to adjust the pH value and resist the browning phenomenon; in addition, low-concentration TDZ (thidiazuron) is used as an induction hormone, so that the embryo induction efficiency is improved.

The invention relates to the technical field of polypropylene material production auxiliaries, in particular to a formula and a preparation method of a compound agent for beta-crystal polypropylene, the formula comprises a compound beta-crystal nucleating agent, a compound beta-crystal stabilizer and a compound antioxidant; the composite beta crystal nucleating agent comprises aryl dicarboxamide and calcium pimelate; the composite beta crystal stabilizer is prepared from beta-(3, 5-di-tert-butyl-4-hydroxyphenyl) propionate and ethylene bis-stearamide (EBS) WPP polypropylene wax. According to the invention, the first nucleating agent aryl dicarboxamide and the second nucleating agent calcium pimelate are matched for use, which is different from the existing scheme and method of independent use, so that the inductivity of the beta crystal is increased; and the beta-(3, 5-di-tert-butyl-4-hydroxyphenyl) propionate serving as a first stabilizer and the ethylene bis-stearamide (EBS) WPP polypropylene wax serving as a second stabilizer are matched for use, so that the beta crystal can be cured, and the produced polypropylene beta crystal is more stable.

The invention provides a culture medium for reducing the content of broussonetia papyrifera esterase and a tissue culture method, and belongs to the technical field of broussonetia papyrifera tissue culture. The culture medium for reducing the content of the broussonetia papyrifera esterase provided by the invention comprises an induction culture medium, a proliferation culture medium, a differentiation proliferation culture medium and a rooting culture medium, wherein the induction culture medium is composed of an MS culture medium, 6-BA and IAA; the proliferation culture medium is composed of a 1 / 2 MS culture medium, NAA, KT and IBA; the differentiation and proliferation culture medium consists of a B5 culture medium, folic acid, TDZ, 2, 4-D, sucrose and agar; the rooting culture medium consists of an N6 culture medium, gibberellin 3, ethylene, IAA, cane sugar and carrageenan. The culture medium and the tissue culture method provided by the invention can promote more than 98% of adventitious buds to root, ensure that the survival rate is more than 98% after acclimatization and transplantation, not only can effectively reduce the content of lignin in paper mulberry tissue culture seedlings, but also cannot influence the normal growth of the paper mulberry tissue culture seedlings.

The invention provides a culture method for obtaining high-quality hemsley rockvine root calluses, belongs to the technical field of tissue culture, and aims to solve the problems of slow growth, high adventitious root occurrence rate, premature aging of the calluses, serious browning, high death rate after subculture and the like in the hemsley rockvine root callus growth process. The culture method comprises the following steps: step 1, pretreatment of explants; step 2, callus induction culture: putting the treated explant into a callus induction culture medium, and putting the callus induction culture medium in an environment with a day-night temperature difference for callus induction culture; and step 3, subculture and enlarged culture of the callus: stripping the callus generated on the stem segment in the step 2, transferring the callus to a subculture multiplication medium, adjusting the light quality ratio of red light to blue light, and carrying out subculture in an environment with gradually changed day temperature to obtain the high-quality hemsley rockvine root callus. In the subculture process, the content of zeatin is increased, the light quality ratio is adjusted, and the high-quality hemsley rockvine root callus can be obtained in a circulation management mode that the daily temperature is gradually changed.

The present application relates to the field of forest tree seedling breeding biotechnology, and specifically to a method for improving the induction rate of embryonic callus of Pinus elliottii or Pinus elliottii, comprising picking cones that meet preset conditions from a donor plant, separating seeds from the cones, peeling off female gametophytes from the seeds, and inducing and culturing the female gametophytes using somatic embryogenesis technology; the preset conditions include: (1) the donor plant is in the induction harvest period; (2) the development status of the picked cones is qualified; the present application solves the problems of poor repeatability and failure of somatic embryogenesis caused by misunderstandings in the traditional method in the judgment of embryonic callus induction and the calculation of induction rate, and provides an important reference for the large-scale production of somatic embryo seedlings of Pinus elliottii or Pinus elliottii based on somatic embryogenesis technology.

The invention relates to the technical field of plant polyploidy breeding, and provides a method for inducing dandelion chromosome doubling by colchicine, which comprises the following steps: sowing and germinating to obtain seedlings, and coating plant growing points of the seedlings with a colchicine solution for induction treatment to obtain dandelion with doubled chromosomes. According to the technical scheme, the problems that traditional breeding of the alkaline land dandelions is difficult, novel breeding technologies are deficient, good varieties are few, biomass of some high-quality varieties is small, and industrial development of the alkaline land dandelions is affected in the prior art are solved.

The present document relates to methods and materials for genome engineering in eukaryotic cells, and in particular to methods of increasing the efficiency of genome engineering (i.e. Transformation or genome editing) by co-delivering one or more chemicals such as a protein deacetylase inhibitor, a plant hormone and / or a regeneration enhancing gene with a genome engineering component.

The present application relates to the technical field of polyploid breeding of shellfish, and specifically discloses a high-efficiency breeding method for triploid Ruditapes philippinarum based on asynchronous acquisition of sperm and egg and early induction. The method comprises the following steps: identifying and separating male and female parent shellfish before inducing parturition; allowing female parent shellfish to naturally release eggs; dissecting male parent shellfish to obtain sperm; artificially mixing and fertilizing; adding cytochalasin B to inhibit the release of the second polar body when the first polar body release rate of the fertilized egg reaches 30%-40%; and washing and hatching to obtain triploid larvae. The present application realizes controllable starting time of fertilization by asynchronous acquisition of sperm and egg, and has a triploid induction rate as high as 92.90% and a standard deviation of only ±1.60% in combination with an early induction window, and has the advantages of stable operation, good repeatability and suitability for large-scale production, thereby providing a reliable technical solution for the genetic improvement and sustainable development of the Ruditapes philippinarum industry.

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.

The invention belongs to the technical field of wheat breeding, and particularly relates to a culture medium for improving wheat anther culture capacity and application of the culture medium to wheat breeding. The invention provides a culture medium for improving wheat anther culture ability. The culture medium is prepared by adding saccharides and Arabic gum into a W14 culture medium, the culture medium suitable for various wheat varieties is obtained through cooperation of the Arabic gum and the carbon source, the culture medium is used for wheat anther culture, the callus induction rate is increased by 4.8 times, and the callus induction rates of the four wheat varieties all reach 50% or above.

The invention discloses a method for rapid propagation of Platycodon grandiflorum by tissue culture, which belongs to the technical field of rapid propagation of plants. The method selects stem segments as explants, provides a callus induction culture medium suitable for Platycodon grandiflorum, and combines a proliferation culture medium with a rooting culture medium. During seedling hardening and transplantation, the cover is not opened to ensure environmental humidity. The transplantation matrix adopts nutrient soil: vermiculite: perlite = 1:1:1. The method greatly improves the propagation coefficient of Platycodon grandiflorum, shortens the propagation time, optimizes the rapid propagation technology of Platycodon grandiflorum by tissue culture in the prior art, significantly saves the cultivation time cost, and is suitable for large-scale rapid tissue culture seedlings of Platycodon grandiflorum.

The invention discloses a tissue culture and rapid propagation method for xanthoceras sorbifolia bunge, and relates to the technical field of biology, and the method is technically characterized in that a traditional breeding mode of xanthoceras sorbifolia bunge is low in efficiency, insufficient in seed source, difficult to survive after transplanting and difficult to preserve excellent characters of a female parent, stem segments and tender stems are used as explants, and the xanthoceras sorbifolia bunge explants are induced to proliferate and root in a short time, so that the xanthoceras sorbifolia bunge is obtained. The induction rate of the callus is improved, browning is reduced, and a solid foundation is laid for construction of a subsequent rapid propagation system of xanthoceras sorbifolia bunge.

The invention discloses a kind of efficient propagation method of Codonopsis pilosula, by using culture medium A in bottle to complete callus induction, then using culture medium B to complete the induction and proliferation steps of adventitious buds, the proliferation coefficient reaches 45.0-50.0;Then cooperate with "micro-cutting" outside the bottle, so that the rooting rate of adventitious buds reaches more than 90%, and subsequent domestication and transplanting steps can be smoothly carried out, and then after continuing to grow and grow, the rooting seedlings with thick roots and strong seedlings are obtained, and the seedling hardening and transplanting are carried out, and the survival rate after transplanting is also more than 90%. The present invention not only simplifies the tissue culture production process of Codonopsis pilosula seedlings, and the proliferation coefficient is much higher than the existing reports of other species of the same family, and the efficient "micro-cutting" rooting system combines adventitious root induction and domestication, greatly shortens the seedling raising cycle of Codonopsis pilosula tissue culture seedlings, solves the low proliferation coefficient in the tissue culture process of Codonopsis pilosula plants in Campanulaceae, the phenomenon such as difficult rooting and domestication, and low cost, short cycle, high quality and survival rate.

The invention provides a preparation method and application of centella asiatica stem cells for high-yield madecassoside, and belongs to the technical field of stem cells. The preparation method comprises the steps of disinfection, induction, proliferation, culture of centella asiatica stem cells with high madecassoside yield and the like, and has the unexpected technical effects that the pollution rate is low, the induction rate is high, the proliferation multiple is high, and the madecassoside content in the obtained centella asiatica stem cells with high madecassoside yield is high. The product is beneficial to industrial production and can be used as a skin care component in a product with anti-inflammatory, soothing and other effects.

The application belongs to the technical field of blueberry cultivation, and discloses a blueberry callus induction method, which comprises the following steps: (1) selecting blueberry flower buds 1-3 days before field flowering, removing petals and stamens, and placing the ovary with the stigma and pedicel retained in a 75% ethanol solution for sterilization for 10-15 min; (2) under aseptic conditions, the ovary with the stigma and pedicel retained is cut to remove the stigma, 1 / 4 of the ovary and the receptacle, and the ovule surrounded on the placenta is carefully taken out with sterilized tweezers and inoculated into a callus induction medium; after 30 days of dark culture, callus is obtained; the application provides a blueberry callus induction method, and has the technical effect of improving the callus induction rate of blueberries.

The invention relates to the field of plant genetic engineering, and discloses a recalcitrant rice variety genetic transformation method based on agrobacterium tumefaciens mediation, which comprises the following steps: 1) carrying out illumination culture on rice seeds in an induction culture medium to obtain calluses; 2) culturing the activated agrobacterium in a suspension culture medium, and adding the callus to obtain an infected callus; (3) carrying out dark culture on the infected callus in a screening culture medium; 4) selecting resistant infection calluses without browning death, and carrying out light-dark alternate culture in a differential medium; and 5) after the resistant infection callus forms a regeneration seedling, transferring the regeneration seedling into a rooting culture medium for alternate light and dark culture to obtain a plant. The efficient, stable and repeatable agrobacterium tumefaciens-mediated genetic transformation method suitable for recalcitrant rice varieties is established by systematically optimizing culture conditions, culture media and the like at the stages of induction, screening, differentiation, rooting and the like, and the transformation efficiency can be remarkably improved.

The invention discloses a method for culturing and transplanting corn transgenic tissue culture seedlings. The method for culturing and transplanting the corn transgenic tissue culture seedlings comprises the steps of immature embryo inoculation, II-type embryonic callus induction, adventitious bud induction, multiplication culture, rooting culture, culture in soil, seedling hardening and transplanting. By adopting the culture and transplanting method provided by the invention, the Zun-dan No.1 corn is used as the explant, and the multiplication culture is carried out under the sterile condition, so that the multiplication is fast, the induction rate is high, the breeding time can be greatly shortened, a large number of tissue culture seedlings can be obtained in a short time, the breeding time is saved, the transgenic breeding process is accelerated, and the method is easy to implement, convenient to operate and suitable for industrial production. A genetic transformation system is easy to establish, technical reference can be provided for exogenous gene transformation and transgenic breeding application of Zun-single No.1 corn, new corn germplasm with beneficial genes can be formed, corn transgenic breeding requirements can be met, the maturing rate of tissue culture seedlings and the seed number of a single plant can be increased, and accordingly planting benefits are improved.

The invention relates to a culture medium combination and method for constructing a zantedeschia aethiopica regeneration system by tubers, the culture medium combination comprises a callus induction culture medium, a proliferation culture medium and a differentiation culture medium, the induction culture medium takes MS as a basic culture medium, and also contains 6.5-7.0 g / L of agar, 30 g / L of sucrose, 1.0-2.0 mg / L of 6-BA and 0.5-1.0 mg / L of NAA; or the culture medium also contains the following substances in concentration: 6.5-7.0 g / L of agar, 30 g / L of cane sugar, 0.5 mg / L of 6-BA and 0.8 mg / L of NAA. According to the method, the sterile bud-free tubers of the colored callalily growing to the specific period are used as the explants, the specific induction culture medium, the proliferation culture medium and the differentiation culture medium are matched to obtain the cluster buds, the callus induction rate is high, and technical support is provided for establishment of a colored callalily tissue culture rapid propagation and genetic transformation system.

The present invention discloses a method for producing tetraploid largemouth black bass by inducing a double hydrostatic shock method. The method first obtains high-quality fertilized eggs through natural fertilization: the steps of injecting hormones and squeezing the abdomen to obtain sperm and eggs in the traditional method are avoided, thereby significantly reducing the damage to the parent fish. Combined with the innovative double hydrostatic shock method, by optimizing the pressure intensity and processing time, the damage to the fertilized eggs is further reduced, the second cleavage is successfully inhibited, and the tetraploid induction rate can be as high as 24.44%. The method can be applied to the tetraploid production of largemouth black bass: the operating method of the present invention is simple and easy, has strong repeatability, and has a high induction rate. These characteristics make the method of the present invention have a high application value in largemouth black bass breeding, and provide strong support for the breeding of tetraploid largemouth black bass.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

The invention discloses an ornamental shallot planting method, and belongs to the technical field of plant rapid propagation, and the method comprises the following steps: (1) explant disinfection; (2) callus induction; (3) differentiation culture; (4) multiplication culture; (5) rooting. According to the ornamental shallot planting method, the reproduction rate of ornamental shallots in each link from explant treatment to callus induction and adventitious bud induction to adventitious bud proliferation and finally to rooting is very high, the characters are stable, the excellent characters of female parents are kept to the maximum extent, and the ornamental shallots have good economic benefits. The invention establishes a good rapid propagation mode of the ornamental green onion, lays a foundation for research work such as new variety cultivation and genetic improvement on the variety by using a plant biotechnology in future, and also provides technical guidance and reference for establishment of a tissue culture rapid propagation technology of other plant varieties.

The invention relates to the technical field of plant tissue culture, and specifically to a method and application of tissue culture rapid propagation of ruby ​​cluster silver leaf immortelle; the steps include: explant selection and cleaning, explant disinfection, primary generation cluster bud induction, secondary generation cluster bud proliferation and cluster bud rooting. The disinfection contamination rate of the method of the invention is 8%, the disinfection mortality rate is 24%, the primary generation cluster bud induction rate is as high as 100%, the comprehensive proliferation coefficient can reach more than 20, the rooting rate stably reaches 100%, and the vitrified seedlings in the secondary generation proliferation process can also be devitrified, and the seedlings are strong and green. The production cycle is greatly shortened, thereby greatly reducing the production cost and management cost.

The invention relates to the technical field of plant tissue culture, and specifically to a method for tissue culture and propagation of East China raspberry and its application; the steps include: selecting explants for cleaning and disinfection, inoculating the disinfected explants in a primary generation cluster bud induction medium for culture, cutting the obtained cluster buds into single buds and inoculating them in a secondary proliferation medium for culture, and cutting the obtained cluster buds into single buds and inoculating them in a rooting medium. The method of the invention reduces the contamination rate to below 5%, increases the cluster bud induction rate from about 10% of a conventional plant growth regulator combination to more than 70%, stabilizes the proliferation coefficient to more than 4, increases the rooting rate to more than 99%, and can also make the seedlings more robust, which is more conducive to the survival of domestication.

The invention discloses a method for producing rice haploids and application of the method in breeding, by editing one or more of OsFEB1, OsFEB2 and OsFEB3 genes in rice, the functions of the OsFEB1, OsFEB2 and / or OsFEB3 genes are lost, a rice haploid induction line is obtained, generation of haploids is induced through sexual reproduction, the gene sequence of the OsFEB1 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, and the gene sequence of the OsFEB3 is as shown in SEQ ID NO.2. And the gene sequence of the OsFEB3 is as shown in SEQ ID NO. 3. The haploid induction line obtained by the method provided by the invention has the capability of inducing generation of haploid plants for breeding. An RHG1 haploid induction line is combined with an MIME system, and an apomixis material is artificially created to fix the heterosis of rice.

The invention discloses a rapid propagation method for tissue culture of phalaenopsis amabilis, which comprises the following steps: selection and treatment of explants: selecting healthy and disease-free explants, and properly treating the explants to improve the success rate of subsequent culture; primary induction culture: putting the treated explant into an induction culture medium to promote primary growth and differentiation; cluster bud induction and subculture multiplication culture: inducing the explant to form a plurality of cluster buds by optimizing culture conditions, and carrying out subculture multiplication culture so as to improve the propagation coefficient; rooting culture: carrying out rooting culture on the formed buds to promote the development of root systems and improve the transplanting survival rate; transplanting and seedling hardening: transplanting the plants with good rooting into a proper matrix, and carrying out seedling hardening to enhance the adaptive capacity of the plants; through the steps, rapid propagation of phalaenopsis amabilis can be achieved, the output period is remarkably shortened, and the production cost is reduced.

The invention relates to a genome editing system and method for melilotus albiflora based on a hairy root genetic transformation system, and belongs to the technical field of plant genetic engineering. According to the invention, an editing carrier system of the MaPDS gene is constructed according to two target sites on the MaPDS gene of the sweet clover, and the editing system is CRISPR2.0-MtLAP1-KO-MaPDS; according to the editing system, sweet clover hypocotyl is used as an explant material, hairy roots are obtained through an agrobacterium rhizogenes-mediated hypocotyl explant infection method, and meanwhile, the hairy roots are obtained on the basis of the principle that anthocyanin is over-expressed and accumulated based on an MtLAP1 transcription factor for color development. Positive hairy roots are obtained through visual, rapid and efficient screening, and efficient editing of the MaPDS gene of the red positive hairy roots is achieved. The method provided by the invention can effectively reduce the occurrence of solid roots in the inducing process of the sweet clover hairy roots, prevent agrobacterium pollution, reduce the detection workload and realize efficient gene editing of target genes.

The invention discloses a seed production method for inducing tetraploid largemouth bass by a dual hydrostatic shock method. According to the method, firstly, high-quality fertilized eggs are obtained through natural fertilization, and the steps of injecting hormone and extruding the abdomen to obtain the fertilized eggs in a traditional method are avoided, so that damage to parent fish is remarkably reduced. In combination with an innovative dual hydrostatic shock method, by optimizing pressure intensity and treatment time, damage to fertilized eggs is further reduced, secondary cleavage is successfully inhibited, and the tetraploid induction rate can reach 24.44%. The method can be applied to tetraploid production of micropterus salmoides. The operation method is simple and easy to implement, high in repeatability and high in induction rate. Due to the characteristics, the method has high application value in breeding of the micropterus salmoides, and powerful support is provided for breeding of the tetraploid micropterus salmoides.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.