7results about How to "High induction rate" patented technology

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.

The application belongs to the technical field of blueberry cultivation, and discloses a blueberry callus induction method, which comprises the following steps: (1) selecting blueberry flower buds 1-3 days before field flowering, removing petals and stamens, and placing the ovary with the stigma and pedicel retained in a 75% ethanol solution for sterilization for 10-15 min; (2) under aseptic conditions, the ovary with the stigma and pedicel retained is cut to remove the stigma, 1 / 4 of the ovary and the receptacle, and the ovule surrounded on the placenta is carefully taken out with sterilized tweezers and inoculated into a callus induction medium; after 30 days of dark culture, callus is obtained; the application provides a blueberry callus induction method, and has the technical effect of improving the callus induction rate of blueberries.

The invention relates to the field of plant genetic engineering, and discloses a recalcitrant rice variety genetic transformation method based on agrobacterium tumefaciens mediation, which comprises the following steps: 1) carrying out illumination culture on rice seeds in an induction culture medium to obtain calluses; 2) culturing the activated agrobacterium in a suspension culture medium, and adding the callus to obtain an infected callus; (3) carrying out dark culture on the infected callus in a screening culture medium; 4) selecting resistant infection calluses without browning death, and carrying out light-dark alternate culture in a differential medium; and 5) after the resistant infection callus forms a regeneration seedling, transferring the regeneration seedling into a rooting culture medium for alternate light and dark culture to obtain a plant. The efficient, stable and repeatable agrobacterium tumefaciens-mediated genetic transformation method suitable for recalcitrant rice varieties is established by systematically optimizing culture conditions, culture media and the like at the stages of induction, screening, differentiation, rooting and the like, and the transformation efficiency can be remarkably improved.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

The invention discloses a method for producing rice haploids and application of the method in breeding, by editing one or more of OsFEB1, OsFEB2 and OsFEB3 genes in rice, the functions of the OsFEB1, OsFEB2 and / or OsFEB3 genes are lost, a rice haploid induction line is obtained, generation of haploids is induced through sexual reproduction, the gene sequence of the OsFEB1 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, and the gene sequence of the OsFEB3 is as shown in SEQ ID NO.2. And the gene sequence of the OsFEB3 is as shown in SEQ ID NO. 3. The haploid induction line obtained by the method provided by the invention has the capability of inducing generation of haploid plants for breeding. An RHG1 haploid induction line is combined with an MIME system, and an apomixis material is artificially created to fix the heterosis of rice.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.

This invention discloses a method for improving the induction rate of prothallus and sporophyte in *Cibotium barometz*, comprising: S1, sterilizing the substrate with a fungicide, wherein the fungicide includes thiophanate-methyl and tebuconazole, and after sterilization, reducing the substrate moisture content to 40-50%, obtaining a sterilized substrate; S2, contacting the bottom of the substrate with a hormone aqueous solution, obtaining a substrate absorbent with the hormone aqueous solution; S3, sowing *Cibotium barometz* spores on the substrate surface for cultivation. This invention shortens the spore germination time of *Cibotium barometz*, improves the induction rate of prothallus and sporophyte, and is simple and easy to implement. Using this method to cultivate *Cibotium barometz* seedlings, prothallus are induced in an average of 22 days, and sporophyte are induced in 66 days. This method can be applied on a large scale to the sowing and seedling cultivation of *Cibotium barometz*, saving seedling time and labor costs.