The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.