154 results about "Hypocotyl" patented technology

The invention belongs to the technical field of crop genetic breeding, and relates to a linked molecular marker of a peanut hypocotyl elongation related gene locus, an identification method and application of the linked molecular marker. The primer pair is C2-15-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C2-15, the peanut hypocotyl length character can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects such as peanut hypocotyl elongation character related gene positioning, genotype typing and later cultivation of excellent peanut varieties.

The invention belongs to the field of crop science and agricultural molecular biology, and particularly relates to application of a BAK gene to enhancement of flood tolerance of rape, and the BAK gene is a BnaA04.BAK7 gene; the login number of the BnaA04. BAK7 gene in a gene pool is BnaA04g015000WE, and the login number of the BnaA04. BAK7 gene in the gene pool is By destroying the expression of the BAK gene, it is found that after the expression of the BAK gene is destroyed, the flooding tolerance of the brassica napus under flooding stress is weakened, and the invention discloses for the first time that hypocotyl growth of the brassica napus under flooding stress is slow and chlorophyll content is reduced due to function deletion of the BAK gene. New gene resources and theoretical guidance are provided for research and application of the flood tolerance of the rape.

The present invention relates to compositions and methods for modifying an elongated hypocotyl 5 (HY5) transcription factor in a plant to inhibit shading responses. The invention also relates to plants and plant parts produced using the methods and compositions of the invention.

The invention discloses an application of prazosin hydrochloride as a strigolactone inhibitor. The piperazosin hydrochloride provided by the scheme of the invention can be used as a plant strigolactone inhibitor for promoting plant branching, regulating the length of a plant hypocotyl and promoting gene expression of key inhibition factors SMXL6, SMXL7 and SMXL8 of strigolactone signal transduction pathways; the plant growth regulator can be applied to various plants, is small in environmental pollution, can be applied to agricultural production in a large scale, and achieves the effects of promoting plant branching, realizing more flowering and more fruiting, improving the stress resistance of the plants, avoiding diseases caused in the pruning process and saving labor consumption.

The invention discloses an agrobacterium rhizogenes-mediated wild jujube seedling hypocotyl in-vivo transgenosis method, and belongs to the technical field of woody plant genetic engineering. The method mainly comprises the following four steps: cultivating wild jujube seedlings, preparing agrobacterium rhizogenes infection liquid containing a GUS label and a ZjMAPKK4 gene, scratching hypocotyl, smearing the agrobacterium rhizogenes infection liquid on a wound to induce hairy roots, and identifying and screening transgenic positive hairy roots. According to the method, wild jujube seedlings serve as materials, agrobacterium rhizogenes serve as mediation, the hypocotyl position is selected to be scratched and infected, and a transgenic material specifically expressed at the root can be rapidly obtained. The method is easy to operate, short in period and low in cost, the process of wild jujube gene function verification can be greatly accelerated, a new method is provided for wild jujube gene function verification, and the method has important significance on wild jujube gene functions and genetic engineering breeding.

The invention relates to the technical field of biology, and particularly discloses an agrobacterium rhizogenes mediated broad bean hairy root induction method without tissue culture, which comprises the following steps: germination of broad bean seeds, transformation of agrobacterium, infection of explants, co-culture of the explants and identification of transgenic broad bean hairy roots. According to the broad bean hairy root induction method provided by the invention, transgenic hairy roots can be obtained only in 2-4 weeks, the method is simple to operate, and tedious sterile operation is avoided; besides, the recombinant agrobacterium rhizogenes of a betaine biosynthesis system RUBY are utilized to infect the broad bean hypocotyl and then culture, so that the broad bean hairy root induction method is successfully established, and the transgenic hairy root can be clearly observed by naked eyes without special equipment or chemical treatment.

The invention discloses a method for inducing multiple types of somatic cells to change fate and directly develop into somatic embryos and application, and belongs to the technical field of plant genetic engineering. According to the invention, functional genes SPCH (AT5G53210) and ARF5 (AT1G19850) capable of promoting conversion of various types of somatic cells into the totipotent stem cells are found. Overexpression of the totipotent regulatory factor LEC2 can induce formation of somatic embryos on arabidopsis sinapis leaves, under the background, the functional genes are subjected to ectopic expression, more arabidopsis sinapis leaf cells can be converted into totipotent stem cells through SPCH overexpression without calluses, and therefore the totipotent stem cells can be used for inducing formation of the somatic embryos on the arabidopsis sinapis leaves. According to the method, more arabidopsis sinapis leaf cells can be transformed into totipotent stem cells, stem tip cells, hypocotyl cells and root cells can be transformed into totipotent stem cells, more arabidopsis sinapis leaf cells can be transformed into totipotent stem cells through ARF5 overexpression, the stem tip cells and the root cells can be transformed into totipotent stem cells, and finally somatic embryos are formed through induction.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a tissue culture and rapid propagation method for hemarthria procumbens. The tissue culture and rapid propagation method comprises the following steps: (1) carrying out sterile treatment on hemarthria procumbens seeds; (2) germination: putting the seeds on an inoculated germination culture medium; (3) induction: cutting a hypocotyl by 1-2cm and putting the hypocotyl into an induction culture medium; (4) proliferation and differentiation: putting into a proliferation and differentiation culture medium; and (5) rooting: finally putting into a rooting culture medium. According to the tissue culture and rapid propagation technology, a large number of hemarthria prostrata plant individuals can be obtained within a short time, dependence on wild hemarthria prostrata resources can be reduced through the tissue culture and rapid propagation technology, protection of the wild plant resources is facilitated, and therefore the requirements of the market for Chinese herbal medicine raw materials are met, and the tissue culture and rapid propagation technology has the technical prospect of large-scale production of pasture raw materials. Meanwhile, the tissue culture and rapid propagation technology of the hemarthria procumbens has wide application prospects in the aspects of promoting sustainable development of the pasture industry, protecting plant resources and promoting scientific research.

The invention relates to the technical field of plant tissue culture, and particularly discloses an establishment method of a grape seedling hypocotyl induced somatic embryo regeneration system. Comprising the following steps: by taking a secondary embryo obtained from a hypocotyl of a seedless grape embryonic-handle malformed seedling as an explant, inducing in a KBN culture medium to obtain an embryonic callus, a non-embryonic callus and a secondary proembryo mass, inoculating the secondary proembryo mass into an X6 culture medium, and carrying out propagation culture to obtain the secondary proembryo mass with increased quality and a somatic embryo, performing secondary induction on the somatic embryos before the cotyledon stage to establish a somatic embryo maintenance and propagation circulating system, inoculating the somatic embryos into an X3 culture medium to differentiate hypocotyls and radicles, and transferring to a WPM culture medium to culture into seedlings. According to the method disclosed by the invention, the hypocotyl of the seedless grape embryonic malformation seedling is successfully induced to obtain a regeneration system, a secondary proembryonic mass maintenance and propagation circulation system is successfully established, and a new acceptor material can be provided for grape genetic transformation.

The invention discloses a method for LbbHLH36 gene transfer of Ningxia wolfberry mediated by Agrobacterium rhizogenes, and relates to the technical field of plant genetic engineering. The method for LbbHLH36 gene transfer of Ningxia wolfberry mediated by Agrobacterium rhizogenes disclosed by the invention is to scratch the hypocotyl of Ningxia wolfberry seedlings with a sterile blade, inoculate the incision with the activated Agrobacterium rhizogenes K599 bacterial solution carrying the target gene recombinant plasmid (35S::eGFP or pRI101-LbbHLH36-eGFP), and cultivate to obtain Ningxia wolfberry transgenic plants. The rolB, NeoR, eGFP, and LbbHLH36 genes in the induced transgenic fluorescent hairy roots of Ningxia wolfberry can all amplify bands, indicating that the above are all transgenic hairy roots induced by Agrobacterium rhizogenes, and it is fast and feasible to transfer the target gene into the root system of Ningxia wolfberry using this system.

The invention discloses a liriodendron hybrids LhDof6 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the Liriodendron hybrids LhDof6 gene disclosed by the invention is shown as SEQ ID NO.1, and the amino acid sequence of the expression protein of the Liriodendron hybrids LhDof6 gene is shown as SEQ ID NO.2. After an expression vector of the hybrid liriodendron LhDof6 gene is constructed, the expression vector is transformed into the hybrid liriodendron; and cultivating and screening to obtain the transgenic hybrid liriodendron plants with obviously improved abiotic stress resistance. The LhDof6 gene disclosed by the invention promotes the extension of hypocotyl of somatic embryo seedlings, so that plant leaves are darker green, and the synthesis of chlorophyll of the plant leaves is promoted; the cold resistance of the hybrid liriodendron plants is improved, and the survival rate of the hybrid liriodendron in an extreme environment is improved; the anti-freezing capability of the plants is improved, and the death rate of the hybrid liriodendron plants after freezing injury is reduced.

The invention belongs to the field of plant tissue culture, and particularly relates to a sainfoin regeneration method which comprises the following steps: inoculating a hypocotyl of a sainfoin sterile seedling as an explant to a regeneration and subculture medium for culture, firstly performing dark culture to obtain an adventitious bud, then performing subculture to obtain a rootless seedling, and performing seedling culture to obtain the sainfoin. And inoculating the rootless seedlings to a rooting culture medium for rooting culture to obtain a sainfoin regeneration plant. According to the method, the time used for constructing a sainfoin regeneration system is greatly shortened, the efficiency is improved, the survival rate of transplanting is high, and a foundation is laid for germplasm resource protection, propagation and subsequent genetic transformation research of sainfoin.

The invention belongs to the technical field of microorganisms, and discloses a Trichoderma brevicompactum 6311 with phytophthora capsici biological control ability, the name of the Trichoderma brevicompactum 6311 is 6311, the classification name of the Trichoderma brevicompactum is Trichoderma brevicompactum 6311, the preservation number of the Trichoderma brevicompactum 6311 is CGMCC No.41489, the preservation date of the Trichoderma brevicompactum 6311 is August 13, 2024, and the preservation unit of the Trichoderma brevicompactum 6311 is China General Microbiological Culture Collection Center, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The fermentation liquor of the trichoderma 6311 has the capability of inhibiting the growth of phytophthora hyphae on a flat plate, and the inhibition rate reaches 100%. The germination rate, radicle and hypocotyl length, growth index and fresh weight of the pepper seeds soaked in the 6311 fermentation liquor are improved. The research shows that the trichoderma brevicompactum 6311 has biological control potential and provides a theoretical basis for application of the trichoderma brevicompactum 6311 as a biological bactericide.

The invention provides an induction method of sesbania hairy roots, which comprises the following steps: beveling hypocotyl of a sesbania seedling, dipping recombinant agrobacterium rhizogenes in the oblique incision of the seedling on the ground, inserting the seedling into vermiculite, and culturing until a new root system is generated, namely the new root system is a sesbania transgenic root system. The method is simple to operate, low in cost and short in time consumption, avoids tedious sterile operation, provides important technical support for molecular biology research of sesbania, and has a relatively great application prospect.

The invention discloses a plant tissue regeneration culture method of angelica sinensis. The method comprises the following steps: obtaining disinfected angelica sinensis seeds, carrying out germination culture on the disinfected angelica sinensis seeds on an induction culture medium to obtain tissue culture seedlings, obtaining hypocotyl of the tissue culture seedlings as explants, then carrying out callus induction culture on the explants on a callus culture medium to obtain calluses, and carrying out tissue culture on the calluses. Performing adventitious bud induction culture on the callus on a subculture medium to obtain adventitious buds, and performing rooting induction culture on the adventitious buds on a rooting culture medium to obtain a complete plant; the angelica sinensis seedlings cultivated by the method are high in survival rate and are not limited by seasons and regions, namely, a large batch of angelica sinensis seedlings can be cultivated, and the method is easy to operate, low in production cost and free of environmental pollution.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The application discloses a SNP molecular marker for identifying hypocotyl length of Brassica campestris L. var. and application thereof, and relates to the technical field of biotechnology.The molecular marker is SNP_LHY0701, which is located on a gene BraC07g017590 of the 7th chromosome of the Brassica campestris L.var., and a SNP site corresponds to a 119th base of a cDNA sequence of the gene BraC07g017590; when the base is C:C, the Brassica campestris L.var. shows long hypocotyls; and when the base is T:T, the Brassica campestris L.var. shows short hypocotyls.The KASP / PARMS technology is applied to gene typing of the SNP molecular marker, the hypocotyl length of the Brassica campestris L.var. can be quickly and accurately detected, the method has strong specificity, high-throughput and rapid detection can be achieved, the method can be simply and quickly applied to molecular-assisted breeding of the Brassica campestris L.var. suitable for mechanical harvesting, and the breeding efficiency is greatly improved.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention relates to the technical field of bioinformatics and crop breeding, and particularly discloses a method, a device and equipment for predicting the close planting yield of brassica napus by using hypocotyl length based on a whole genome selection technology. The method is characterized by comprising the following steps: acquiring whole genome sequencing data of brassica napus germplasm; inputting the whole genome sequencing data into a target model; outputting hypocotyl length data of the brassica napus under a close planting condition through the target model; and according to the hypocotyl length data of the brassica napus under the close planting condition, obtaining a brassica napus germplasm yield potential sorting result. According to the method, whole genome selection and key morphological characters (hypocotyl length) under specific stress (close planting conditions) are combined for the first time, high-yield varieties suitable for close planting cultivation can be efficiently and accurately screened out without on-site close planting tests in the early stage of rape breeding, and the breeding efficiency is remarkably improved.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The invention relates to the technical field of biology, in particular to an agrobacterium tumefaciens-mediated open cotton hairy root transformation method which comprises the following steps: selecting eight-day-old cotton seedlings, transversely cutting a hypocotyledonary axis 0.5-1cm above a rhizome junction, and cutting off an underground part; then, agrobacterium rhizogenes liquid is injected into the notch of the hypocotyl, the notch of the hypocotyl is subjected to vacuum filtration treatment, then seedlings are cut into wet vermiculite and placed in a seedling raising tray, a proper amount of clear water is added into the seedling raising tray, the seedling raising tray is covered with a moisturizing cover, dark treatment is conducted for 24 h, and then light culture is conducted in the environment with the relative humidity being 65-75%; an efficient induction transformation system of the cotton hairy roots under the open condition can be established. The method for rapidly inducing the cotton transgenic hairy roots under the open condition is established, sterile operation is not needed, a culture medium does not need to be used, cotton genotype limitation is avoided, and a simple, convenient and efficient research method is provided for cotton gene function analysis and root system biology research.

The application discloses a method for cultivating and obtaining gene edited Punica granatum by a two-step somatic embryogenesis technique, which comprises the following steps: material obtaining, obtaining of embryogenic callus, preparation of Agrobacterium tumefaciens liquid, pre-culturing of callus, infection, co-culturing, washing, selection culture, subculture and ex vitro rooting, etc. The application firstly takes Punica granatum stem segments as explants to establish a Punica granatum gene editing plant system and obtain Punica granatum gene editing plants, avoids the separation of offspring traits when taking Punica granatum seed hypocotyls as explants, and lays a good foundation for subsequent researches on Punica granatum gene function and fine variety cultivation; in addition, the application further refines the somatic embryogenesis conditions under the selection pressure of Kan and TMT on the basis of the established Punica granatum somatic embryogenesis, directly transforms embryogenic cells by using the bipolar characteristics of somatic embryos, and thus complete and stable Punica granatum gene editing plants are obtained, and the transformation rate is high.

The application discloses an Arabidopsis beta-ketoacyl-ACP reductase gene promoter and application thereof. The promoter has the nucleotide sequence shown in SEQ ID No. 1, and controls the Arabidopsis beta-ketoacyl-ACP reductase gene to be specifically expressed in the hypocotyl and root of the Arabidopsis seedling, the leaf and pistil stigma of the seedling, and the fungal infection site of the seedling leaf after inoculation of powdery mildew (Erysiphe cichoracearum, UCSC1). G. cichoracerum The application provides a new tissue-specific and white-powder-fungus-infection-specifically-induced expression promoter, and provides a new regulating element for improving the genetic engineering breeding of the plant resistance to the white-powder fungus.

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

The invention relates to a construction method of an efficient regeneration system of idesia polycarpa, which comprises the following steps: soaking idesia polycarpa seeds in 3% gibberellic acid diluted by 1000 times for 3 days, sterilizing, inoculating to a germination culture medium of 1 / 2MS, 1.0 mg / L 6-benzylamino adenine (6-BA) and 0.5 mg / L 3-indolebutyric acid (IBA), and culturing for 25 days to obtain seedlings; a hypocotyledonary axis at the position 2 cm below the cotyledon of the seedling is obliquely cut and inoculated to a culture medium containing MS, 1.0 mg / L of thidiazuron (TDZ) and 0.5 mg / L of IBA, and calluses are induced after 30 days; inoculating the calluses to a culture medium containing MS, 2.0 mg / L TDZ and 0.05 mg / L IBA, and differentiating regenerated buds after 30 days; after seedling strengthening and rooting culture, the seedlings are transplanted to a sterilization matrix with the ratio of nutrient soil to vermiculite to perlite being 4: 1: 1. The differentiation rate of regenerated seedlings reaches 64.82% and is remarkably higher than that in the prior art, the seed germination rate and the transplanting survival rate are high, and an idesia polycarpa regeneration system can be efficiently constructed.

This invention discloses the application of the rice LOC_Os01g67410 gene in plant response to high ambient temperature. PLT5 It is a negative regulatory factor for deep-seeded rice seedlings. The length of internodes and mesocotyls during the seedling stage... plts The mutant showed no significant difference in germination speed and germination rate under shallow sowing conditions compared to the wild type, but under deep sowing conditions, it germinated faster and had a higher germination rate than the wild type. Furthermore, although a warm environment of 32℃ promoted earlier and faster germination time for both the wild type and the mutant, the germination rate of the wild type decreased significantly at 32℃. plts The mutant was still able to maintain a high germination rate. plts The mutant's tolerance to deep sowing and strong emergence performance suggest its important application value in dry direct sowing, especially in high-temperature summer direct sowing production. This can be achieved by finding mutants in natural populations or cultivars. PLTs The superior haplotype will be beneficial for breeding rice varieties suitable for deep sowing.

The invention discloses a method for establishing a gleditsia sinensis tissue culture regeneration system, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) inoculating explants from wild gleditsia sinensis seeds into an adventitious bud induction culture medium, and performing induction culture to generate adventitious buds; and (2) cutting off the adventitious bud from the base, inserting the cut end into a root induction culture medium, and inducing rooting. According to the method, hypocotyls and cotyledons of gleditsia sinensis aseptic seedlings are used as explants, and the influence of different plant growth regulator combinations, browning inhibitors and the like on the regeneration efficiency is discussed. According to the method, an efficient gleditsia sinensis regeneration system is successfully established, and a theoretical basis and a technical support are provided for high-quality seedling breeding and genetic engineering genetic improvement.

The invention discloses a breeding method of a potherb mustard variety suitable for mechanical harvesting, which comprises the following steps: taking a cytoplasmic male sterile line 08-5-6A of a potherb mustard local variety straw mustard as a female parent and taking a Shaoxing potherb mustard local variety brassica juncea selfing line 18-3-21-29-3-1-3 as a male parent for hybrid seed production to breed a new potherb mustard variety Yongxue No.15 with an upright and compact plant type. The Yongxue No.15 bred by the breeding method is upright and compact in plant type, bolting-resistant, 2-3cm in overground and hypocotyl length and suitable for mechanical harvesting, the plant height is more than 50cm, the weight of a single plant is more than 1kg, the yield per mu is more than 6000kg, and wide planting of the bred Yongxue No.15 has important significance for promoting industrial science and technology progress and high-quality development.