102 results about "Hypocotyl" patented technology

The invention relates to the technical field of biology, and particularly discloses an agrobacterium rhizogenes mediated broad bean hairy root induction method without tissue culture, which comprises the following steps: germination of broad bean seeds, transformation of agrobacterium, infection of explants, co-culture of the explants and identification of transgenic broad bean hairy roots. According to the broad bean hairy root induction method provided by the invention, transgenic hairy roots can be obtained only in 2-4 weeks, the method is simple to operate, and tedious sterile operation is avoided; besides, the recombinant agrobacterium rhizogenes of a betaine biosynthesis system RUBY are utilized to infect the broad bean hypocotyl and then culture, so that the broad bean hairy root induction method is successfully established, and the transgenic hairy root can be clearly observed by naked eyes without special equipment or chemical treatment.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The application discloses a SNP molecular marker for identifying hypocotyl length of Brassica campestris L. var. and application thereof, and relates to the technical field of biotechnology.The molecular marker is SNP_LHY0701, which is located on a gene BraC07g017590 of the 7th chromosome of the Brassica campestris L.var., and a SNP site corresponds to a 119th base of a cDNA sequence of the gene BraC07g017590; when the base is C:C, the Brassica campestris L.var. shows long hypocotyls; and when the base is T:T, the Brassica campestris L.var. shows short hypocotyls.The KASP / PARMS technology is applied to gene typing of the SNP molecular marker, the hypocotyl length of the Brassica campestris L.var. can be quickly and accurately detected, the method has strong specificity, high-throughput and rapid detection can be achieved, the method can be simply and quickly applied to molecular-assisted breeding of the Brassica campestris L.var. suitable for mechanical harvesting, and the breeding efficiency is greatly improved.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention relates to the technical field of bioinformatics and crop breeding, and particularly discloses a method, a device and equipment for predicting the close planting yield of brassica napus by using hypocotyl length based on a whole genome selection technology. The method is characterized by comprising the following steps: acquiring whole genome sequencing data of brassica napus germplasm; inputting the whole genome sequencing data into a target model; outputting hypocotyl length data of the brassica napus under a close planting condition through the target model; and according to the hypocotyl length data of the brassica napus under the close planting condition, obtaining a brassica napus germplasm yield potential sorting result. According to the method, whole genome selection and key morphological characters (hypocotyl length) under specific stress (close planting conditions) are combined for the first time, high-yield varieties suitable for close planting cultivation can be efficiently and accurately screened out without on-site close planting tests in the early stage of rape breeding, and the breeding efficiency is remarkably improved.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The application discloses a method for cultivating and obtaining gene edited Punica granatum by a two-step somatic embryogenesis technique, which comprises the following steps: material obtaining, obtaining of embryogenic callus, preparation of Agrobacterium tumefaciens liquid, pre-culturing of callus, infection, co-culturing, washing, selection culture, subculture and ex vitro rooting, etc. The application firstly takes Punica granatum stem segments as explants to establish a Punica granatum gene editing plant system and obtain Punica granatum gene editing plants, avoids the separation of offspring traits when taking Punica granatum seed hypocotyls as explants, and lays a good foundation for subsequent researches on Punica granatum gene function and fine variety cultivation; in addition, the application further refines the somatic embryogenesis conditions under the selection pressure of Kan and TMT on the basis of the established Punica granatum somatic embryogenesis, directly transforms embryogenic cells by using the bipolar characteristics of somatic embryos, and thus complete and stable Punica granatum gene editing plants are obtained, and the transformation rate is high.

The application discloses an Arabidopsis beta-ketoacyl-ACP reductase gene promoter and application thereof. The promoter has the nucleotide sequence shown in SEQ ID No. 1, and controls the Arabidopsis beta-ketoacyl-ACP reductase gene to be specifically expressed in the hypocotyl and root of the Arabidopsis seedling, the leaf and pistil stigma of the seedling, and the fungal infection site of the seedling leaf after inoculation of powdery mildew (Erysiphe cichoracearum, UCSC1). G. cichoracerum The application provides a new tissue-specific and white-powder-fungus-infection-specifically-induced expression promoter, and provides a new regulating element for improving the genetic engineering breeding of the plant resistance to the white-powder fungus.

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

The invention relates to a construction method of an efficient regeneration system of idesia polycarpa, which comprises the following steps: soaking idesia polycarpa seeds in 3% gibberellic acid diluted by 1000 times for 3 days, sterilizing, inoculating to a germination culture medium of 1 / 2MS, 1.0 mg / L 6-benzylamino adenine (6-BA) and 0.5 mg / L 3-indolebutyric acid (IBA), and culturing for 25 days to obtain seedlings; a hypocotyledonary axis at the position 2 cm below the cotyledon of the seedling is obliquely cut and inoculated to a culture medium containing MS, 1.0 mg / L of thidiazuron (TDZ) and 0.5 mg / L of IBA, and calluses are induced after 30 days; inoculating the calluses to a culture medium containing MS, 2.0 mg / L TDZ and 0.05 mg / L IBA, and differentiating regenerated buds after 30 days; after seedling strengthening and rooting culture, the seedlings are transplanted to a sterilization matrix with the ratio of nutrient soil to vermiculite to perlite being 4: 1: 1. The differentiation rate of regenerated seedlings reaches 64.82% and is remarkably higher than that in the prior art, the seed germination rate and the transplanting survival rate are high, and an idesia polycarpa regeneration system can be efficiently constructed.

This invention discloses the application of the rice LOC_Os01g67410 gene in plant response to high ambient temperature. PLT5 It is a negative regulatory factor for deep-seeded rice seedlings. The length of internodes and mesocotyls during the seedling stage... plts The mutant showed no significant difference in germination speed and germination rate under shallow sowing conditions compared to the wild type, but under deep sowing conditions, it germinated faster and had a higher germination rate than the wild type. Furthermore, although a warm environment of 32℃ promoted earlier and faster germination time for both the wild type and the mutant, the germination rate of the wild type decreased significantly at 32℃. plts The mutant was still able to maintain a high germination rate. plts The mutant's tolerance to deep sowing and strong emergence performance suggest its important application value in dry direct sowing, especially in high-temperature summer direct sowing production. This can be achieved by finding mutants in natural populations or cultivars. PLTs The superior haplotype will be beneficial for breeding rice varieties suitable for deep sowing.

The invention discloses a method for establishing a gleditsia sinensis tissue culture regeneration system, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) inoculating explants from wild gleditsia sinensis seeds into an adventitious bud induction culture medium, and performing induction culture to generate adventitious buds; and (2) cutting off the adventitious bud from the base, inserting the cut end into a root induction culture medium, and inducing rooting. According to the method, hypocotyls and cotyledons of gleditsia sinensis aseptic seedlings are used as explants, and the influence of different plant growth regulator combinations, browning inhibitors and the like on the regeneration efficiency is discussed. According to the method, an efficient gleditsia sinensis regeneration system is successfully established, and a theoretical basis and a technical support are provided for high-quality seedling breeding and genetic engineering genetic improvement.

The invention discloses a breeding method of a potherb mustard variety suitable for mechanical harvesting, which comprises the following steps: taking a cytoplasmic male sterile line 08-5-6A of a potherb mustard local variety straw mustard as a female parent and taking a Shaoxing potherb mustard local variety brassica juncea selfing line 18-3-21-29-3-1-3 as a male parent for hybrid seed production to breed a new potherb mustard variety Yongxue No.15 with an upright and compact plant type. The Yongxue No.15 bred by the breeding method is upright and compact in plant type, bolting-resistant, 2-3cm in overground and hypocotyl length and suitable for mechanical harvesting, the plant height is more than 50cm, the weight of a single plant is more than 1kg, the yield per mu is more than 6000kg, and wide planting of the bred Yongxue No.15 has important significance for promoting industrial science and technology progress and high-quality development.

The invention relates to an oximido triazole compound as well as a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The oximido triazole compound disclosed by the invention has a vigorous growth control effect, can effectively control vigorous growth of crops such as peanuts, rice and soybeans, improves the yield and quality of the crops, and has the characteristics of strong vigorous growth control effect, small application amount, safety to the crops and the like. Experimental results show that the oximido triazole compound has the effects of inhibiting the plant height of rice and promoting the root growth of rice, has the effects of inhibiting the hypocotyl length of cucumber and tomato seedlings, and has the effects of inhibiting the vigorous growth of peanut plants and improving the chlorophyll content, the fresh fruit weight and the fruit number of peanuts.

The invention relates to a method for increasing plant growth by administering phytoinitiator mRNAs obtained from seedling chloroplasts. The mRNAs can either be translated directly into chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts as proteins. In both cases, the resulting proteins lead to the activation of hormonal signaling pathways, increased protein synthesis, and a significant increase in root biomass, shoot number, and hypocotyl growth. The invention further comprises formulations and application systems such as granules, sprays, or injections that enable efficient uptake and action of the mRNAs.

The invention relates to a plant grafting technology in agricultural horticulture, in particular to a bacterial liquid preparation for promoting healing of grafting unions of muskmelons and pumpkins and application of the bacterial liquid preparation, and provides a bacterial liquid preparation which is simple in operation method, outstanding in application effect and capable of promoting healing of grafting unions of the muskmelons and the pumpkins according to physiological and molecular mechanisms of CmGH9B3 infection liquid in grafting healing of the muskmelons and the pumpkins. The bacterial liquid preparation can significantly improve the grafting healing efficiency of muskmelon and pumpkin and the quality of grafted seedlings. In the attaching grafting process of the muskmelon and the pumpkin, the muskmelon scion hypocotyl is soaked in a bacterial liquid preparation to be infected for 30 min, the muskmelon scion hypocotyl and the pumpkin rootstock are fixed through a grafting clamp, callus formation at a grafting opening and connection between the scion and a vascular bundle bridge of the rootstock can be promoted, and then healing of the grafting opening of the muskmelon and the pumpkin is accelerated; the grafting healing rate and survival rate are improved, and the grafted seedling quality is guaranteed.

The invention relates to the technical field of plant genetic engineering, in particular to application of a plant HSF family transcription factor HSFA2B in regulation and control of temperature response growth of plant mesocotyl. The invention provides application of a plant HSF family transcription factor HSFA2B in regulating and controlling temperature response growth of mesocotyl in a plant seedling stage or regulating and controlling direct seeding emergence of plants at high environment temperature. The invention finds that HSFA2B has an important function in rice mesocotyl response temperature growth regulation and control. The mesocotyl of the mutant with the HSFA2B knocked out is obviously longer than that of the wild type at the proper environment temperature, but the mesocotyl of the mutant with the HSFA2B knocked out is not obviously different from that of the wild type at the high environment temperature, and the response sensitivity to the high environment temperature is reduced. The discovery of the new function of the HSFA2B is of great significance to the breeding and direct seeding production of rice direct seeding varieties.

The invention relates to a method for increasing plant growth by delivering phyto-initiator mRNAs derived from chloroplasts of seedlings. The mRNAs may either be translated directly in chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts in the form of proteins. In both cases, the resulting proteins activate hormonal signalling pathways, increased protein synthesis, and bring about a significant enhancement of root biomass, shoot number and hypocotyl growth. The invention also relates to formulations and application systems such as granules, sprays or injections which enable efficient uptake and activity of the mRNAs.

The invention belongs to the technical field of gene engineering, and particularly relates to a method for knocking out a brassica napus BnaFAH gene by utilizing a CRISPR-Cas9 system and application. Two copies of the brassica napus BnaFAH gene are knocked out at a fixed point by using a CRISPR-Cas9 system, two target fragments are selected from conserved regions of fourth and sixth exons of the two copies of the brassica napus BnaFAH gene, BnaFAH-Cas9 plant expression vectors are respectively constructed, the BnaFAH-Cas9 plant expression vectors are introduced into brassica napus hypocotyl callus through an agrobacterium infection method, and seedlings are regenerated. Two copies of the BnaFAH gene are subjected to double-strand shearing under the combined action of two sgRNAs and Cas9 nuclease, random deletion on a target gene segment is finally realized through a cell self-repairing function, gene knockout is completed, a mutant plant is obtained, the mutant plant obtained after the BnaFAH gene is knocked out is verified to enhance the resistance to plutella xylostella, and the mutant plant has a good application prospect. Therefore, the gene is a key gene for regulating and controlling the insect resistance of the brassica napus, and has important significance for breeding new insect-resistant varieties of the brassica napus.

The invention relates to the technical field of biology, in particular to a Chinese cabbage hypocotyl length related molecular marker and application thereof. The invention firstly provides an application of the molecular marker related to the length of the hypocotyl of the Chinese cabbage or a substance for detecting the molecular marker related to the length of the hypocotyl of the Chinese cabbage in identification or auxiliary identification of the length of the hypocotyl of the Chinese cabbage. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to a difference between sequence fragments of 49-420 sites of SEQ ID No.1 and sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to the difference between the sequence fragments of 49-420 sites of SEQ ID No.1 and the sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage can be used for identifying or assisting in identifying the length of the hypocotyl of the Chinese cabbage in a seedling stage, and the breeding process of a new variety of the long-hypocotyl Chinese cabbage is accelerated.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a method for establishing a tissue culture system of Rheum tanguticum. The culture system comprises taking leaves, petioles and hypocotyls of Rheum tanguticum as explants, using MS medium, and sequentially carrying out induction of callus, adventitious buds and adventitious roots, and through adding different concentrations and different combinations of hormones, it is determined that the leaves and petioles are induced into callus, and then the propagation of Rheum tanguticum is realized in the form of artificial seeds, the hypocotyls can be directly induced into adventitious buds, or can be induced into callus first, then into adventitious buds, and finally into adventitious roots, so as to realize the cultivation of Rheum tanguticum. Through the above method, the application of Rheum tanguticum tissue is widened, so that each part can be used for plant regeneration culture, and the regenerated plant of Rheum tanguticum is successfully obtained, and the tissue culture system of Rheum tanguticum is formed, which lays a foundation for the artificial rapid propagation and genetic improvement of Rheum tanguticum.

The invention discloses application of a keratin related protein gene GbKAP related to cotton fiber quality characters, and belongs to the field of biotechnology application. The growth vigor and plant height of root cells, main roots, hypocotyls and rosette leaves of an arabidopsis thaliana plant for heterologous expression of the cotton keratin related protein gene GbKAP are superior to those of a control group. The GbKAP plays an important role and has an application prospect in promoting cell elongation, improving the quality character of cotton fibers and cultivating new varieties of high-quality cotton fibers.

The application provides an application of azelaic acid in promoting seed germination and / or plant growth, and belongs to the technical field of plant growth regulation. The application not only provides the application of azelaic acid in promoting seed germination and / or plant growth, but also provides a method for promoting seed germination and / or plant growth. The seed is treated with an azelaic acid solution with a concentration of 0.1-1.0 mM before sowing. The results of examples show that the azelaic acid can effectively improve the germination rate of the seed under salt stress and normal conditions, shorten the seed germination time, promote the hypocotyl growth and root growth of seedlings, and relieve the adverse effects of salt stress environment on seed germination and plant growth.

The invention relates to the field of medicinal plant tissue culture, in particular to a nitraria davidii culture medium and a culture method and application thereof. Comprising the following steps: S1, sequentially carrying out concentrated sulfuric acid treatment and chlorine fumigation on sophora davidii seeds to obtain sterile seedlings; s2, cutting the hypocotyls of the aseptic seedlings obtained in the step S1 into segments, and inoculating the segments to a callus induction culture medium; the callus induction culture medium is prepared from the following main components: an MS culture medium, 17 to 23 g / L of cane sugar, 1.5 to 2.5 mg / L of 6-BA and 0.1 to 0.4 mg / L of NAA; s3, inoculating the callus obtained in the step S2 to a callus proliferation culture medium; the callus proliferation culture medium comprises the following main components: an MS culture medium, 17 to 23 g / L of sucrose, 0.3 to 3.5 mg / L of 6-BA and 0.5 to 3.5 mg / L of NAA. The production cost and the environmental risk can be obviously reduced while the quality and the survival rate of tissue culture seedlings are improved.

The invention provides a hybrid potato seedling hypocotyl single cell extraction method and application thereof. According to the characteristics of potato seedling hypocotyl single cells, the extraction efficiency of the protoplast is remarkably improved by adjusting the dosage of different cellulase in the enzymatic hydrolysate and combining a slight extrusion mode. The method is simple and rapid, does not need complex treatment and expensive reagents, and is convenient to popularize and apply. Besides, experiments prove that the protoplast extracted by the method can be effectively used for detection such as subsequent sequencing and the like, so that the cells extracted by the method are relatively high in activity and have a wide application value.

The invention discloses a method for cultivating strong towel gourd seedlings through low-temperature treatment. The method comprises the following steps of shell breaking, wherein the edges of seeds are broken; seeds are soaked with warm water, and then the seeds are fished out, cleaned and spin-dried; accelerating germination, namely accelerating germination of the soaked seeds at 28-30 DEG C; sprouting: when the seeds begin to sprout, picking out the seeds which just sprout in batches; low-temperature treatment: placing the seeds with white buds under the low-temperature condition of 3-4 DEG C, and storing in a shading manner for 2 days; sowing: sowing the seeds subjected to low-temperature treatment in a seedbed, and covering with soil after sowing; and seedling cultivation: cultivating the seedlings into towel gourd seedlings. According to the method, the stage after germination and before sowing is selected, the method of treating the germinated seeds at low temperature is adopted, the storage time of the germinated seeds is prolonged, the radicle length is controlled, the germination potential is promoted to be relatively consistent, during one-time sowing, the seedling plant height and the hypocotyl length are reduced, the strong seedling index is increased, the seedling group growth vigor and quality are neat and consistent in appearance, and strong seedling formation is promoted.

The invention provides a grape somatic embryo regeneration method, and belongs to the technical field of grape tissue culture. The grape somatic embryo regeneration method comprises the steps of seed germination, embryogenic callus induction, somatic embryo differentiation by the embryogenic callus, somatic embryo rooting induction and seedling hardening. According to the method, the hypocotyl of the grape plant is selected as the explant, and the specific plant growth regulator is added into the induction culture medium and the somatic embryo differentiation culture medium, so that the callus induction rate and the somatic embryo occurrence rate are increased. According to the method, a large number of grape somatic embryo regeneration plants with consistent hereditary basis can be obtained, rapid, stable and efficient seedling culture of grapes can be realized, the period of grape breeding is shortened, and a new method is provided for preparing the grape somatic embryo regeneration plants.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor MYB46 to regulation and control of temperature response growth of mesocotyl of a plant. It is found that the plant MYB family transcription factor MYB46 is a negative regulation factor responding to the rice mesocotyl at the high environment temperature, the mesocotyl length of the MYB46 knockout mutant is remarkably longer than that of a wild type at the high environment temperature, the MYB46 knockout mutant is more sensitive to the high environment temperature response, and the MYB46 has important application value in direct seeding production of rice at the high temperature in summer. The discovery of the new function of the MYB46 provides new gene resources and methods for cultivating rice varieties suitable for direct seeding.