432 results about "Hypocotyl" patented technology

Composition enriched with natural phyto-estrogens or analogs thereof selected from Genistein, Daidzein, Formomometin, and Biochanin A. These may be used as food additives, tablets, or capsules for promoting health in cases of cancer, premenstrual syndrome, menopause, or hypercholestrolaemia.

The invention discloses a method for grafting melons on double rootstocks, comprising the following steps: (1) simultaneously sowing the rootstocks cucurbita moschata and cucurbita ficifolia in pairs; (2) sowing the seeds of the melons 5-8 days after sowing the rootstocks; (3) when the diameter of the first mainleaf of the rootstock cucurbita moschata is 4.5-6cm, removing the first mainleaf of the cucurbita moschata, removing the growing point of the cucurbita ficifolia and grafting the rootstocks by inarching, and meanwhile, bevelling the parts of the lower hypocotyl, 1.5-2.5cm from the cotyledon, of the seedlings of the melons and obliquely inserting the bevelled scions of the melons on the parts of the lower hypocotyl, 0.3-0.7cm from the cotyledon, of the cucurbita moschata by the grafting needles; and (4) managing the seedlings and planting the seedlings 2-4 days later. The method realizes simultaneous inarching of the two rootstocks and cuttage grafting of the scions of the melons and the rootstocks, greatly improves the operation efficiency, greatly lowers the consequent management cost and is very applicable to industrial production of the seedlings raised by grafting.

The invention relates to a method for high-frequency regenerating Chinese sapium bark through tissue culturing adventitious bud. The invention is characterized in that it comprises following steps: removing seed coat from Chinese sapium bark's seed, disinfecting surface with hydrogen dioxide solution and inoculating seed germinating culture medium, and getting intact Chinese sapium bark seed seedling; cutting the cotyledon of seed seedling, apical bud containing undeveloped true leaf and hypocotyl and root, and then inoculating them into adventitious bud inducing culture medium suitable for their growth, inducing adventitious bud directly or inducing with callus and getting light green adventitious bud cluster; replanting it into adventitious bud lengthening culture medium, getting lengthened adventitious bud seedling; replanting into seedling breeding culture medium for enlarged breeding, and replanting it into rooting culture medium, getting strong tube Chinese sapium bark with developed true leaf; finally replanting it out of room for natural growth and getting strong Chinese sapium bark seedling.

A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

The invention relates to an agrobacterium rhizogenes-mediated and vacuum infiltration-assisted soybean genetic transformation method, and belongs to the technical fields of molecular biology and genetic engineering. The agrobacterium rhizogenes-mediated and vacuum infiltration-assisted soybean genetic transformation method comprises the following steps of: selecting genotypes of soybean seeds, disinfecting the soybean seeds, and germinating; preparing bacterial liquid, which contains anti-nematode bacillus thuringiensis (Bt) genes, of agrobacterium rhizogene K599; transforming the agrobacterium rhizogenes-mediated soybeans; performing histochemical staining analysis on transgenic roots; and performing polymerase chain reaction (PCR) detection on the transgenic roots. According to the method, an agrobacterium rhizogenes-mediated and vacuum infiltration-assisted transformation method is applied to the soybeans for the first time, and hairy roots of soybean roots are transformed successfully; the disinfected soybean seeds are planted in sterile vermiculite and are germinated, the injury of epidermic meristematic cells is caused at the upper end of hypocotyl, the agrobacterium rhizogene is infected under the vacuum condition, and genetic transformation is performed to obtain the soybean transgenic hairy roots containing the anti-nematode BT genes. By implementing the agrobacterium rhizogenes-mediated and vacuum infiltration-assisted soybean genetic transformation method, the opportunity of the invasion of the agrobacterium rhizogenes can be improved fully, the infection efficiency of the agrobacterium rhizogenes can be improved obviously, and the transformation rate of the soybeans is improved effectively.

The invention relates to a new application of sodium hydrosulfide for promoting the morphogenesis of plant roots, namely, the application is that the sodium hydrosulfide is taken as root stimulate to promote the morphogenesis of side roots and adventitious roots of the seedling of the plant and the morphogenesis of the adventitious roots of cutting branches of the plant. The sodium hydrosulfide has obvious root-promoting effect to the seedlings of the plant such as rapes, mung beans, soybeans, cucumbers and the like, the hypocotyledonary axis of the cut segment thereof and the cutting branches of the plant such as willow branches, peach branches, grapes, batatas and the like, promotes the formation and growth of the side roots and the adventitious roots of the plant, enhances the water absorbing capacity of the plant, maintains the water balance in the body of the plant, reduces membrane lipid overoxidation damage of the hypocotyledonary axis of the cut segment and the cutting branches, maintains the relative stability of membrane structure of the explant and promotes the growth and propagation of the plant. The root-promoting agent can be used for growing roots of various plant seedlings, the hypocotyledonary axis of the cut segment and the cutting branches and is applicable to the root-promoting effect of promoting and culturing the seedlings of transplanting and cutting and the like, in the agriculture, forestry and gardening.

The invention discloses application of a method for smearing and transforming agrobacterium rhizogene-mediated hypocotyl to soybean transformation. The method for smearing and transforming agrobacterium rhizogen-mediated hypocotyl to is applied to soybeans for the first time, and hairy root transformation of soybean roots is successfully carried out. In this invention, the sprouting time of seeds and the concentration of screening agents are optimized, therefore not only increasing the quantity of transgenic hairy roots induced by each soybean explant, but also obviously enhancing the transformation frequency and growth conditions of hairy roots. A screening marker weed killer usually used for soybean transformation is successfully used for obtaining soybean compound plants in aseptic conditions, wherein each soybean compound plant has a transgenic root and a non-transgenic over-ground part and can be used as the ideal material for studying genes relative to the form of root systems, the symbiotic nitrogen fixation of roots and the formation of fungus roots.

A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

The invention discloses an aseptic grafting method solving the difficulty of soybean transformed adventitious buds in rooting. Hypocotyls of soybean seedlings aseptically germinated are used as rootstocks. Genetically transformed soybean buds are scions to be grafted to the rootstocks. The buds grafted with the scions are provided with radicles below the hypocotyls with no need of reforming a root system, grafting is performed aseptically with no need of tedious operations of binding and fixing the scions and rootstocks, the buds which are grafted with the scions can heal with the rootstocks to grow into complete plants able to survive in transplanting and can also grow, develop, flower and fruit normally, and survival rate of grafted seedlings is 100%. The technical problem that the soybean transformed adventitious buds root difficulty is solved effectively.

The invention discloses an agrobacterium tumefaciens mediated cabbage type oilseed rape genetic transformation method. The method comprises the following steps: taking the hypocotyl of a cabbage type oilseed rape seedling, conducting preculture through adopting the lower end of the hypocotyl as an explant, and inducing for production of the callus; infecting the callus explant by adopting agrobacterium tumefaciens carrying target genes, and conducting the co-culture; after the co-culture is conducted on the explant, inducing the explant to generate resistance adventitious buds on a differential medium containing a selective agent; conducting the rooting culture to obtain a resistance seedling. According to the agrobacterium tumefaciens mediated cabbage type oilseed rape genetic transformation method, the lower end of the cabbage type oilseed rape hypocotyl serves as the explant, and the growth conditionis adopted, which is most suitable for cabbage type oilseed rape genetic transformation, and particularly includes the pH value and the high concentration glyphosate differentiation screening method and the low concentration glyphosate differentiation screening method in the co-culture medium, so that the transgenetic seedling with glyphosate resistance is obtained successfully; compared with the prior art, the method has higher resistance seedling differentiation rate, a high efficiency cabbage type oilseed rape regeneration system with glyphosate resistance is built, and a foundation is laid for studying about the transgenic technology of the agrobacterium tumefaciens mediated cabbage type oilseed rape.

The invention discloses an ex-vitro rooting method for tissue culture seedlings of pinus massoniana. The ex-vitro rooting method comprises the following steps: taking terminal buds of aseptic seedlings with 0.5cm long hypocotyls as explants for inducing cluster buds in a culture medium I; carrying out elongation culture on the induced cluster buds in a culture medium II, individually cutting off the terminal buds, and inoculating the cut terminal buds into a culture medium III for propagation to obtain subculture buds I; repeatedly elongating the subculture buds I, individually cutting off the subculture buds I, dividing the cut subculture buds I into terminal buds and bud sections, continuously propagating in a subculture propagation culture medium III to obtain subculture buds II, and repeatedly elongating and propagating the subculture buds II to obtain new propagation subculture buds; and transplanting the individually cut 2.5-3.5cm high subculture buds into a seedling culture cup for ex-vitro rooting, and transplanting the ex-vitro subculture buds into a seedling nursery for culture to obtain the ex-vitro tissue culture seedlings. According to the method disclosed by the invention, the production program of test-tube seedlings is simplified, the production cost is reduced, the production efficiency is improved, the rooting treatment time is 25-30 days, the ex-vitro rooting rate reaches 92%-98%, the root system quality is higher, the transplanting survival rate reaches 90%-95%, and the method has better economic, social and ecologic benefits.

The invention discloses a method and application of knocking out a BnMAX1 gene in Brassica napus by a CRISPR-Cas9 system, two sgRNAs of specifically targeting Brassica napus BnMAX1 gene are designed and synthesized into oligo dimer, are connected with Cas9 vector, and introduced into hypocotyl callus of Brassica napus by Agrobacterium tumefaciens-mediated transformation to regerated into shoots. Cas9 nuclease was guided by sgRNA to cleave target sequence. Each sgRNA could pass through CRISPR-Cas9 system mediates the cleavage of BnMAX1 gene in A and C genomes, and achieves the goal of gene knockout. Phenotypic identification showed that homozygous mutant lines increased the number of branches and pods per plant, decreased plant height and increased yield.

The invention relates to a mangrove plant northward movement and domestication method. The method sequentially includes the following steps of firstly, collecting mangrove plant hypocotyls good in development, soaking the hypocotyls in local seawater, and transporting the hypocotyls to a planting land as soon as possible; secondly, inserting and planting the mangrove plant hypocotyls in the planting land; finally, constructing a greenhouse in the planting land before winter to ensure that tidewater can normally rise and fall in the greenhouse, and conducting high-temperature and low-temperature alternating domestication culture on the mangrove plant hypocotyls inserted and planted in the planting land. By means of the method, autumn eggplant seedlings can be domesticated under the naturalconditions, the environment temperature is continuously adjusted through the greenhouse in the seedling breeding process in winter, on one hand, the autumn eggplant seedlings are protected against theinfluences of extreme weathers, and on the other hand, the seedlings slowly adapt to the cold weather, and the adaptability to low temperature of the seedlings is improved; the seedlings are domesticated on the aspects of environment temperature, seawater salinity and soil texture, the survival rate of mangrove plants in high-latitude regions is increased, and mangrove forests can grow in high-latitude regions from 28 degrees northern latitude to 31 degrees northern latitude.

The invention relates to a cold-resistant acclimation method for kandelia candel seedling pot plants planted at a high latitude. The method successively comprises the following steps that firstly, kandelia candel hypocotyls developing well are collected, soaked by using seawater and transplanted to a planting field as soon as possible; secondly, flower pots are placed on the planting field and then filled with planting soil; then, the kandelia candel hypocotyls are inserted and planted in the flower pots; finally, before winter, a greenhouse is built on the planting field, it is guaranteed that tidewater can normally rise and fall to the interior of the greenhouse, and the kandelia candel hypocotyls which are inserted and planted in the flower pots are subjected to high-temperature and low-temperature alternative acclimation cultivation. According to the cold-resistant acclimation method, in the natural mudflat environment, a potting technology is used for seedling raising and acclimation at the same time, the survival rate is increased, and afforestation through kandelia candel is achieved in harsh mudflats; in the seedling raising process in winter, the environmental temperatureof the greenhouse is continuously adjusted, so that seedlings gradually adapt to the cold climate, the adaptive capacity to the lower temperature is improved, and the mangrove forest grows in a high-latitude area with the north latitude of 28-31 degrees.

The invention relates to a method for cultivating and producing polygalacic acid with polygala root hair-shaped roots, which uses polygala root hypocotyl and blades as an explantation and converts by activating hair-shaped root agrobacterium, and leads T-DNA of hair-shaped root agrobacterium Ri plasmid to be integrated to polygala root nucleus DNA for expressing and forming hair-shaped roots, and polygala root hair-shaped roots are gained through plural times of subinoculation. The polygala root hair-shaped roots which are gained contain the same components of polygalacic acid (namely, senega-saponin) as that of traditional Chinese medicine polygala root, and the content is not lower than the wild traditional Chinese medicine polygala root. The invention can be used as new medicine resources to provide polygala root products, which solves the practical problems that the demand amount of polygala root is increased year by year, and wild resources are lacked and the quality of cultivated breeds is reduced, which brings practical problems to clinic, Chinese patent drug processes and export.

The invention discloses a grafting method for model arabidopsis thaliana.The method comprises the following steps that an arabidopsis thaliana plant with the seedling age of 6-8 days, thick and strong hypocotyl explant, consistent growth vigour and a main leaf emerging newly is used for grafting; stock and scion materials used for grafting are placed in the middle of 1.2% sepharose gel, the hypocotyl explant is transversely cut, and it is ensured that the hypocotyl explant part of the scion material is short and the hypocotyl explant part of the stock material is long; material notches are in tight contact on the sepharose gel, a 1/2MS+3% saccharose solid culture medium with the area larger than the stock root area is cut to cover the stock root, and a grafting body is formed; the grafting body is placed in the environment under the temperature of 21 DEG C to be vertically cultured for 7 days; after grafting notches are healed, the grafting body is transferred into a water culture device with 1/4MS as a liquid culture medium to be cultured.The problems that operation is difficult, notches are not round or normal, scion side roots are frequently produced, and the grafting survival rate is low due to the fact that arabidopsis thaliana plants are thin and weak are solved.

The present invention provides an isolated nucleic acid including a nucleotide sequence encoding COL8, which controls hypocotyl length and flowering time in a plant. It is found that plants over-expressing COL8 under long day conditions show slight hypocotyl elongation and delayed flowering in comparison with the wild type. In addition, COL8 and FKF1, which is a circadian-clock related gene, are found to be localized at the same site in a plant cell, indicating the existence of an interaction between the two.

A method for fast regeneration of lavender includes such steps as taking the hypocotyl from the germinated seed of lavender, direct differentiating in the regenerating solid culture medium to generate adventitious bud, and rooting in the rooting solid culture medium to obtain complete plant. Its advantages are short period (60 days), and high germination percentage (46.7%) and rooting rate (95%).

A means for enhancing the yield of a harvesting part of a crop is provided. A yield enhancing agent for a harvesting part selected from the group consisting of seed, fruit, hypocotyl, root and stem in a crop, the yield enhancing agent comprising: 5-aminolevulic acid or a derivative thereof represented by the following formula (1), or a salt thereof; and a gibberellin biosynthesis inhibitor: R²R¹NCH₂COCH₂CH₂COR³ (1), wherein R¹ and R² each independently represent a hydrogen atom, an alkyl group, an acyl group, an alkoxycarbonyl group, an aryl group or an aralkyl group; and R³ represents a hydroxyl group, an alkoxy group, an acyloxy group, an alkoxycarbonyloxy group, an aryloxy group, an aralkyloxy group or an amino group, wherein the yield enhancing agent is an agent to be applied at the time when the harvesting part of the crop becomes enlarged.

The invention relates to a cultivation system and a method for mangrove seedling distributed in the north of the border of mangroves. The cultivation system for mangrove seedling comprises waste heat recycling system and several sets of incubators; the incubator comprises mangrove plants, floating island sheet, planting cups, a cultivation case body, a supporting plate for floating island sheet, an emptying pipe, a vertical drain,a siphon standpipe, a blow-off pipe, overflow pipe, a water inlet. The waste heat recycling system provides warm water for each sets of the incubators. The cultivation method for mangrove seedling comprises the following steps: warm water source is produced by waste heat recycling system; sea mud is placed into the planting cup; environmental adaptation, sterilization and pest control are performed on the hypocotyl of mangrove plants and the mangrove plants are planted into planting cups; warm water is injected into the cultivation box; overflow of the overflow pipe is regularly checked and drain contamination treatment is carried out. The cultivation system and method for mangrove seedling fully utilize the waste heat resource, ensure the suitable water temperature for planting mangrove seedlings, realize the artificial cultivation of mangrove seedlings in high latitudes, and purify the water by using the developed roots of mangrove plants.

The invention belongs to tissue culture, in particular to a common head cabbage tissue culture method. The method comprises the process steps of aseptic seedling culture, induction culture, multiplication culture, rooting culture, regeneration seedling transplanting and the like. The method provided by the invention solves the problems that in the prior art, the subculture, the long-time storage and the rooting transplanting and utilization of common head cabbage tissue culture seedlings cannot be favorably realized, and the like. When being adopted, the method has the advantages that callus tissue can grow on cotyledons on a common head cabbage induction culture medium, hypocotyls can be directly differentiated into adventitious buds, the tissue culture seedling multiplication times is 3.8 to 4.6, the growth condition of the tissue culture seedlings is strong, vitrification seedlings are few, the multiplication rate is high, and the rooting rate of the tissue culture seedlings can reach 100 percent, and the like.

The invention discloses an alfalfa genetic inverting method in plant transferring gene technical domain, which comprises the following steps: 1, comprising two methods; evoking callus with alfalfa hypocotyls through floating culture mode; assisting by super sound; sieving body embryo in medium; germinating the body embryo; generating root; getting the transforming plant; 2, using tender true leaf of alfalfa as transferring acceptor material; transforming with agricillin medium guild; sieving in traumatic evoked medium; seeding resistance callus in differential medium; evoking out of body embryo; generating root; getting the transforming plant. This invention increases genetic transferring efficiency of alfalfa and shorten genetic transferring period.

A method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien comprises the steps as follows: taking aseptic seedling of the catharanthus roseus, and obtaining hypocotyl of the aseptic seedling; preparing bacteria solution of the agrobacterium tumefacien and utilizing the agrobacterium tumefacien for inducing transformation of the hypocotyl of catharanthus roseus; taking and placing the hypocotyl on a callus induction culture medium for culture to obtain a callus; inoculating the callus onto a differential culture medium for culture to obtain a green callus; inoculating the green callus onto an indefinite bud induction culture medium for culture to obtain an indefinite bud; and taking and placing the indefinite bud into a radication culture medium for culture to obtain the catharanthus roseus transgenic plants. In the method, a stable genetic transformation system of the catharanthus roseus induced by the agrobacterium tumefacien is established, therefore the method lays a foundation for further study on metabolic pathway control net and for the improvement of TIAs content, and can be further applied to gene engineering seed breeding for producing TIAs metabolite.