68 results about "Hypocotyl" patented technology

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The invention relates to the technical field of bioinformatics and crop breeding, and particularly discloses a method, a device and equipment for predicting the close planting yield of brassica napus by using hypocotyl length based on a whole genome selection technology. The method is characterized by comprising the following steps: acquiring whole genome sequencing data of brassica napus germplasm; inputting the whole genome sequencing data into a target model; outputting hypocotyl length data of the brassica napus under a close planting condition through the target model; and according to the hypocotyl length data of the brassica napus under the close planting condition, obtaining a brassica napus germplasm yield potential sorting result. According to the method, whole genome selection and key morphological characters (hypocotyl length) under specific stress (close planting conditions) are combined for the first time, high-yield varieties suitable for close planting cultivation can be efficiently and accurately screened out without on-site close planting tests in the early stage of rape breeding, and the breeding efficiency is remarkably improved.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The application discloses an Arabidopsis beta-ketoacyl-ACP reductase gene promoter and application thereof. The promoter has the nucleotide sequence shown in SEQ ID No. 1, and controls the Arabidopsis beta-ketoacyl-ACP reductase gene to be specifically expressed in the hypocotyl and root of the Arabidopsis seedling, the leaf and pistil stigma of the seedling, and the fungal infection site of the seedling leaf after inoculation of powdery mildew (Erysiphe cichoracearum, UCSC1). G. cichoracerum The application provides a new tissue-specific and white-powder-fungus-infection-specifically-induced expression promoter, and provides a new regulating element for improving the genetic engineering breeding of the plant resistance to the white-powder fungus.

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

The invention relates to a construction method of an efficient regeneration system of idesia polycarpa, which comprises the following steps: soaking idesia polycarpa seeds in 3% gibberellic acid diluted by 1000 times for 3 days, sterilizing, inoculating to a germination culture medium of 1 / 2MS, 1.0 mg / L 6-benzylamino adenine (6-BA) and 0.5 mg / L 3-indolebutyric acid (IBA), and culturing for 25 days to obtain seedlings; a hypocotyledonary axis at the position 2 cm below the cotyledon of the seedling is obliquely cut and inoculated to a culture medium containing MS, 1.0 mg / L of thidiazuron (TDZ) and 0.5 mg / L of IBA, and calluses are induced after 30 days; inoculating the calluses to a culture medium containing MS, 2.0 mg / L TDZ and 0.05 mg / L IBA, and differentiating regenerated buds after 30 days; after seedling strengthening and rooting culture, the seedlings are transplanted to a sterilization matrix with the ratio of nutrient soil to vermiculite to perlite being 4: 1: 1. The differentiation rate of regenerated seedlings reaches 64.82% and is remarkably higher than that in the prior art, the seed germination rate and the transplanting survival rate are high, and an idesia polycarpa regeneration system can be efficiently constructed.

The invention discloses a method for establishing a gleditsia sinensis tissue culture regeneration system, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) inoculating explants from wild gleditsia sinensis seeds into an adventitious bud induction culture medium, and performing induction culture to generate adventitious buds; and (2) cutting off the adventitious bud from the base, inserting the cut end into a root induction culture medium, and inducing rooting. According to the method, hypocotyls and cotyledons of gleditsia sinensis aseptic seedlings are used as explants, and the influence of different plant growth regulator combinations, browning inhibitors and the like on the regeneration efficiency is discussed. According to the method, an efficient gleditsia sinensis regeneration system is successfully established, and a theoretical basis and a technical support are provided for high-quality seedling breeding and genetic engineering genetic improvement.

The invention relates to a method for increasing plant growth by administering phytoinitiator mRNAs obtained from seedling chloroplasts. The mRNAs can either be translated directly into chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts as proteins. In both cases, the resulting proteins lead to the activation of hormonal signaling pathways, increased protein synthesis, and a significant increase in root biomass, shoot number, and hypocotyl growth. The invention further comprises formulations and application systems such as granules, sprays, or injections that enable efficient uptake and action of the mRNAs.

The invention relates to the technical field of plant genetic engineering, in particular to application of a plant HSF family transcription factor HSFA2B in regulation and control of temperature response growth of plant mesocotyl. The invention provides application of a plant HSF family transcription factor HSFA2B in regulating and controlling temperature response growth of mesocotyl in a plant seedling stage or regulating and controlling direct seeding emergence of plants at high environment temperature. The invention finds that HSFA2B has an important function in rice mesocotyl response temperature growth regulation and control. The mesocotyl of the mutant with the HSFA2B knocked out is obviously longer than that of the wild type at the proper environment temperature, but the mesocotyl of the mutant with the HSFA2B knocked out is not obviously different from that of the wild type at the high environment temperature, and the response sensitivity to the high environment temperature is reduced. The discovery of the new function of the HSFA2B is of great significance to the breeding and direct seeding production of rice direct seeding varieties.

The invention relates to a method for increasing plant growth by delivering phyto-initiator mRNAs derived from chloroplasts of seedlings. The mRNAs may either be translated directly in chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts in the form of proteins. In both cases, the resulting proteins activate hormonal signalling pathways, increased protein synthesis, and bring about a significant enhancement of root biomass, shoot number and hypocotyl growth. The invention also relates to formulations and application systems such as granules, sprays or injections which enable efficient uptake and activity of the mRNAs.

The invention relates to the technical field of biology, in particular to a Chinese cabbage hypocotyl length related molecular marker and application thereof. The invention firstly provides an application of the molecular marker related to the length of the hypocotyl of the Chinese cabbage or a substance for detecting the molecular marker related to the length of the hypocotyl of the Chinese cabbage in identification or auxiliary identification of the length of the hypocotyl of the Chinese cabbage. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to a difference between sequence fragments of 49-420 sites of SEQ ID No.1 and sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to the difference between the sequence fragments of 49-420 sites of SEQ ID No.1 and the sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage can be used for identifying or assisting in identifying the length of the hypocotyl of the Chinese cabbage in a seedling stage, and the breeding process of a new variety of the long-hypocotyl Chinese cabbage is accelerated.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a method for establishing a tissue culture system of Rheum tanguticum. The culture system comprises taking leaves, petioles and hypocotyls of Rheum tanguticum as explants, using MS medium, and sequentially carrying out induction of callus, adventitious buds and adventitious roots, and through adding different concentrations and different combinations of hormones, it is determined that the leaves and petioles are induced into callus, and then the propagation of Rheum tanguticum is realized in the form of artificial seeds, the hypocotyls can be directly induced into adventitious buds, or can be induced into callus first, then into adventitious buds, and finally into adventitious roots, so as to realize the cultivation of Rheum tanguticum. Through the above method, the application of Rheum tanguticum tissue is widened, so that each part can be used for plant regeneration culture, and the regenerated plant of Rheum tanguticum is successfully obtained, and the tissue culture system of Rheum tanguticum is formed, which lays a foundation for the artificial rapid propagation and genetic improvement of Rheum tanguticum.

The application provides an application of azelaic acid in promoting seed germination and / or plant growth, and belongs to the technical field of plant growth regulation. The application not only provides the application of azelaic acid in promoting seed germination and / or plant growth, but also provides a method for promoting seed germination and / or plant growth. The seed is treated with an azelaic acid solution with a concentration of 0.1-1.0 mM before sowing. The results of examples show that the azelaic acid can effectively improve the germination rate of the seed under salt stress and normal conditions, shorten the seed germination time, promote the hypocotyl growth and root growth of seedlings, and relieve the adverse effects of salt stress environment on seed germination and plant growth.

The invention relates to the field of medicinal plant tissue culture, in particular to a nitraria davidii culture medium and a culture method and application thereof. Comprising the following steps: S1, sequentially carrying out concentrated sulfuric acid treatment and chlorine fumigation on sophora davidii seeds to obtain sterile seedlings; s2, cutting the hypocotyls of the aseptic seedlings obtained in the step S1 into segments, and inoculating the segments to a callus induction culture medium; the callus induction culture medium is prepared from the following main components: an MS culture medium, 17 to 23 g / L of cane sugar, 1.5 to 2.5 mg / L of 6-BA and 0.1 to 0.4 mg / L of NAA; s3, inoculating the callus obtained in the step S2 to a callus proliferation culture medium; the callus proliferation culture medium comprises the following main components: an MS culture medium, 17 to 23 g / L of sucrose, 0.3 to 3.5 mg / L of 6-BA and 0.5 to 3.5 mg / L of NAA. The production cost and the environmental risk can be obviously reduced while the quality and the survival rate of tissue culture seedlings are improved.

The invention provides a hybrid potato seedling hypocotyl single cell extraction method and application thereof. According to the characteristics of potato seedling hypocotyl single cells, the extraction efficiency of the protoplast is remarkably improved by adjusting the dosage of different cellulase in the enzymatic hydrolysate and combining a slight extrusion mode. The method is simple and rapid, does not need complex treatment and expensive reagents, and is convenient to popularize and apply. Besides, experiments prove that the protoplast extracted by the method can be effectively used for detection such as subsequent sequencing and the like, so that the cells extracted by the method are relatively high in activity and have a wide application value.

The invention discloses a method for cultivating strong towel gourd seedlings through low-temperature treatment. The method comprises the following steps of shell breaking, wherein the edges of seeds are broken; seeds are soaked with warm water, and then the seeds are fished out, cleaned and spin-dried; accelerating germination, namely accelerating germination of the soaked seeds at 28-30 DEG C; sprouting: when the seeds begin to sprout, picking out the seeds which just sprout in batches; low-temperature treatment: placing the seeds with white buds under the low-temperature condition of 3-4 DEG C, and storing in a shading manner for 2 days; sowing: sowing the seeds subjected to low-temperature treatment in a seedbed, and covering with soil after sowing; and seedling cultivation: cultivating the seedlings into towel gourd seedlings. According to the method, the stage after germination and before sowing is selected, the method of treating the germinated seeds at low temperature is adopted, the storage time of the germinated seeds is prolonged, the radicle length is controlled, the germination potential is promoted to be relatively consistent, during one-time sowing, the seedling plant height and the hypocotyl length are reduced, the strong seedling index is increased, the seedling group growth vigor and quality are neat and consistent in appearance, and strong seedling formation is promoted.

The invention relates to the technical field of plant genetic engineering, in particular to application of a transcription factor MYB46 to regulation and control of temperature response growth of mesocotyl of a plant. It is found that the plant MYB family transcription factor MYB46 is a negative regulation factor responding to the rice mesocotyl at the high environment temperature, the mesocotyl length of the MYB46 knockout mutant is remarkably longer than that of a wild type at the high environment temperature, the MYB46 knockout mutant is more sensitive to the high environment temperature response, and the MYB46 has important application value in direct seeding production of rice at the high temperature in summer. The discovery of the new function of the MYB46 provides new gene resources and methods for cultivating rice varieties suitable for direct seeding.

The present application relates to the technical field of mangrove ecological restoration, and discloses a mangrove ecological restoration method based on a beach suitable for forest land, which comprises the following steps: (1) constructing a suitable forest land; S1, fixing mud with pine piles: two layers of pine piles are punched at a distance of 30-50 cm from the periphery of the area to be restored, and the beach mud is fixed; S2, filling mud to construct a planting base: the beach mud is filled in the area to be restored to construct a planting base, and a beach suitable for forest land is obtained; S3, constructing a wave protection dike: two layers of bamboo fences are inserted around the area to be restored, and oyster shells and silt are filled in the bamboo fences at intervals to form an oyster reef wave protection dike; (2) planting mangroves; S4, selecting and breeding: two or more seedlings are selected for breeding to obtain hypocotyl seedlings or seedlings; S5, combined planting: two or more seedlings are planted by means of interplanting, and mangroves are obtained. The present application effectively improves the speed of mangrove ecological construction and restoration by reasonably configuring pine piles and oyster reef wave protection dikes.

The invention provides a pumpkin hypocotyl color related KASP molecular marker which corresponds to a mutation site of a CmoCh19G004530 gene on a No.19 chromosome of a pumpkin. The mutation site is located at the first basic group of the second exon of the moCh19G004530 gene and corresponds to the 5502973 site of the No.19 chromosome of the pumpkin, and the basic group of the site is mutated from T to C. The invention also provides a primer group for detecting the molecular marker, and the sequence of the primer group is as shown in SEQ ID NO.3-5. In addition, the invention also provides application of the molecular marker or the primer group and a pumpkin hypocotyl color identification method. The early identification of pumpkin hypocotyl color, germplasm resource screening and variety breeding are carried out based on the molecular marker disclosed by the invention, so that the accuracy of identification of a black-stem pumpkin material and the efficiency of germplasm resource creation can be remarkably improved, meanwhile, the breeding period of a black-stem pumpkin variety is shortened, the breeding precision is improved, and the cost is reduced.

This invention relates to the field of plant growth regulation technology, specifically disclosing a cultivation method for promoting the germination of wild sea buckthorn seeds. The main inventive concept lies in the synergistic application of amino oligosaccharides (AS) and sodium nitroprusside (SNP) in promoting the germination and hypocotyl elongation of wild sea buckthorn seeds. Using AS alone (optimal concentration 0.12 mmol / L) or SNP alone (optimal concentration 0.09 mmol / L) can significantly improve the germination rate (GP) and germination index (GI) of sea buckthorn seeds, while simultaneously promoting hypocotyl elongation. Combining AS (0.12 mmol / L) and SNP (0.08 mmol / L) in a 1:1 ratio produces a synergistic effect, promoting the germination of wild sea buckthorn seeds, increasing seed α-amylase activity, and promoting hypocotyl elongation. This invention provides a low-cost, high-efficiency technical solution for efficient sea buckthorn seedling cultivation, applicable to ecological restoration and economic forest cultivation.

The application discloses a method for delaying leaf senescence of Brassica chinensis var. rosularis by editing genes SGR1 and SGR2, and belongs to the technical field of crop breeding. The method comprises the following steps: designing specific sgRNA according to the coding region sequence of the stay-green genes SGR1 and SGR2 of the Brassica chinensis var. rosularis, constructing a CRISPR / Cas9 gene editing vector, transforming the Brassica chinensis var. rosularis receptor material by means of Agrobacterium tumefaciens infection of the cotyledon and hypocotyl, obtaining the transformed plant containing the gene editing element through adventitious bud regeneration and screening, and screening the plant with the SGR1 and / or SGR2 gene site-directed mutation through molecular identification and phenotype observation. The application precisely modifies the stay-green genes by the gene editing technology, significantly delays the leaf senescence process of the Brassica chinensis var. rosularis, improves the leaf stay-green property and the preservation period, and keeps excellent agronomic traits, thereby providing a new way for the genetic improvement and preservation technology development of the Brassica chinensis var. rosularis, and having important application value in the fields of vegetable production, preservation and breeding.

The invention discloses application of CsHEC1 protein and a coding gene thereof in regulating and controlling the length of a hypocotyl of a cucumber, and further relates to a creation method of a short hypocotyl cucumber material and a corresponding new germplasm. A CsHEC1 gene is knocked out with a specific target by adopting a CRISPR / Cas9 technology, and a CsHEC1 homozygous mutation cucumber plant is obtained through vector construction, agrobacterium tumefaciens-mediated cucumber genetic transformation and screening identification. The hypocotyl of the plant seedling is obviously shorter than that of a wild type, the excessive growth problem in the seedling raising stage can be avoided, additional environment regulation or chemical reagents are not needed, the cost and the phytotoxicity risk are reduced, and gene resources and technical support are provided for cucumber breeding and efficient seedling raising.

The invention relates to the technical field of horticultural cultivation, in particular to a culture method for increasing the growth rate of crassulaceae ambrosia succulent plants. Comprising the following steps: selecting crassulaceae kalanchoe plants with developed root systems as stocks; after top cutting, naturally air-drying for 3-6 days at the temperature of 20-25 DEG C and the humidity of 70-80% to form a cut wooden bolt sealing layer; the method comprises the following steps: selecting a blister succulent plant with a hypocotyledonary axis as a scion; inserting the scion into a stock cut by adopting a cleft grafting method for fixing; and then placing in a short-day weak-light environment with the sunshine duration of 3-5 hours and the light intensity of 5000-7000lx for healing culture. According to the method, the technical bottleneck of extremely slow growth of blister succulent plants is thoroughly broken through through precise cooperation of strong nutrient pumping of the heterogeneity rootstocks, specific air-drying corrosion prevention and transpiration inhibition by weak light, black rot of grafted water is effectively avoided, the cultivation period is shortened to one year from several years, and the method has great large-scale production value.

The present application relates to BnAHL15 gene and its application in regulating early flowering of plants, especially the application of BnAHL15 gene of rapeseed in regulating early flowering of plants, and belongs to the technical field of biology.The present application firstly designs sgRNA which is specific to BnAHL15 gene, makes oligo dimer of the sgRNA, and constructs a gene editing vector with a Cas9 skeleton, and then introduces the gene editing vector into hypocotyl callus of Brassica napus by means of agrobacterium-mediated genetic transformation technology, and generates seedlings again, under the guidance of sgRNA, Cas9 nuclease edits BnAHL15 gene on the genome, produces a frameshift mutation, causes early termination of gene coding, and phenotype identification finds that the flowering period of homozygous mutant lines of rapeseed is advanced, and the size (yield) of seeds does not change obviously.

The invention discloses carbon dot hydrogen-rich water for promoting garlic sprout growth and a preparation method and application thereof. Hydrogen is dissolved in the carbon dot hydrogen-rich water. According to the method, carbon dots are used as a material for stabilizing hydrogen and preventing hydrogen escape, hydrogen escape can be remarkably slowed down after the carbon dots are combined with hydrogen-rich water, the hydrogen content is increased, the carbon dots jointly act on garlic germination and seedling growth and development, and meanwhile the inhibition effect under salt stress treatment is relieved. The carbon dots and the hydrogen-rich water are combined to serve as a soil conditioner and a plant growth regulator, and the synergistic effect of the carbon dots and the hydrogen-rich water has the effects of improving salt resistance and increasing the germination rate and the growth speed. Hydrogen in the carbon dot hydrogen-rich water can help plants to resist oxidative stress, regulate plant growth and development, improve soil environment, enhance plant stress resistance, improve plant quality and enhance plant tolerance to stress; the fluorescence property of the carbon dots can improve the absorbance and photosynthesis of plant mineral elements, accelerate seed germination and promote seedling root systems and hypocotyls to extend.

The invention discloses a method for improving alkali resistance of cotton. The method comprises the step of treating cotton seeds with a procyanidine solution, wherein the concentration of the procyanidine solution is 1-10 mM. The method disclosed by the invention is suitable for treating conventional upland cotton, such as Ji 228, Zhong H242, Xinautumn No.1, glyphosate resistance, Zhong 9807, Zhong 9835, Zhong S 9612, 2012 HB-5, HB09, Lu 8H7H5 and the like. As the growth stage of hypocotyl and radicle after seed germination is the key stage of stress-resistant growth of cotton plants, the germinated seeds are used for experiments, and a method for enhancing the alkali resistance of cotton in the salinization environment by adding procyanidine is established, so that the inhibition of hypocotyl and radicle during cotton germination can be relieved.

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.