123 results about "Hypocotyl" patented technology

The invention belongs to the technical field of crop genetic breeding, and relates to a linked molecular marker of a peanut hypocotyl elongation related gene locus, an identification method and application of the linked molecular marker. The primer pair is C2-15-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C2-15, the peanut hypocotyl length character can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects such as peanut hypocotyl elongation character related gene positioning, genotype typing and later cultivation of excellent peanut varieties.

The invention belongs to the field of crop science and agricultural molecular biology, and particularly relates to application of a BAK gene to enhancement of flood tolerance of rape, and the BAK gene is a BnaA04.BAK7 gene; the login number of the BnaA04. BAK7 gene in a gene pool is BnaA04g015000WE, and the login number of the BnaA04. BAK7 gene in the gene pool is By destroying the expression of the BAK gene, it is found that after the expression of the BAK gene is destroyed, the flooding tolerance of the brassica napus under flooding stress is weakened, and the invention discloses for the first time that hypocotyl growth of the brassica napus under flooding stress is slow and chlorophyll content is reduced due to function deletion of the BAK gene. New gene resources and theoretical guidance are provided for research and application of the flood tolerance of the rape.

The invention relates to the technical field of biology, and particularly discloses an agrobacterium rhizogenes mediated broad bean hairy root induction method without tissue culture, which comprises the following steps: germination of broad bean seeds, transformation of agrobacterium, infection of explants, co-culture of the explants and identification of transgenic broad bean hairy roots. According to the broad bean hairy root induction method provided by the invention, transgenic hairy roots can be obtained only in 2-4 weeks, the method is simple to operate, and tedious sterile operation is avoided; besides, the recombinant agrobacterium rhizogenes of a betaine biosynthesis system RUBY are utilized to infect the broad bean hypocotyl and then culture, so that the broad bean hairy root induction method is successfully established, and the transgenic hairy root can be clearly observed by naked eyes without special equipment or chemical treatment.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a tissue culture and rapid propagation method for hemarthria procumbens. The tissue culture and rapid propagation method comprises the following steps: (1) carrying out sterile treatment on hemarthria procumbens seeds; (2) germination: putting the seeds on an inoculated germination culture medium; (3) induction: cutting a hypocotyl by 1-2cm and putting the hypocotyl into an induction culture medium; (4) proliferation and differentiation: putting into a proliferation and differentiation culture medium; and (5) rooting: finally putting into a rooting culture medium. According to the tissue culture and rapid propagation technology, a large number of hemarthria prostrata plant individuals can be obtained within a short time, dependence on wild hemarthria prostrata resources can be reduced through the tissue culture and rapid propagation technology, protection of the wild plant resources is facilitated, and therefore the requirements of the market for Chinese herbal medicine raw materials are met, and the tissue culture and rapid propagation technology has the technical prospect of large-scale production of pasture raw materials. Meanwhile, the tissue culture and rapid propagation technology of the hemarthria procumbens has wide application prospects in the aspects of promoting sustainable development of the pasture industry, protecting plant resources and promoting scientific research.

The invention discloses a plant tissue regeneration culture method of angelica sinensis. The method comprises the following steps: obtaining disinfected angelica sinensis seeds, carrying out germination culture on the disinfected angelica sinensis seeds on an induction culture medium to obtain tissue culture seedlings, obtaining hypocotyl of the tissue culture seedlings as explants, then carrying out callus induction culture on the explants on a callus culture medium to obtain calluses, and carrying out tissue culture on the calluses. Performing adventitious bud induction culture on the callus on a subculture medium to obtain adventitious buds, and performing rooting induction culture on the adventitious buds on a rooting culture medium to obtain a complete plant; the angelica sinensis seedlings cultivated by the method are high in survival rate and are not limited by seasons and regions, namely, a large batch of angelica sinensis seedlings can be cultivated, and the method is easy to operate, low in production cost and free of environmental pollution.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The application discloses a SNP molecular marker for identifying hypocotyl length of Brassica campestris L. var. and application thereof, and relates to the technical field of biotechnology.The molecular marker is SNP_LHY0701, which is located on a gene BraC07g017590 of the 7th chromosome of the Brassica campestris L.var., and a SNP site corresponds to a 119th base of a cDNA sequence of the gene BraC07g017590; when the base is C:C, the Brassica campestris L.var. shows long hypocotyls; and when the base is T:T, the Brassica campestris L.var. shows short hypocotyls.The KASP / PARMS technology is applied to gene typing of the SNP molecular marker, the hypocotyl length of the Brassica campestris L.var. can be quickly and accurately detected, the method has strong specificity, high-throughput and rapid detection can be achieved, the method can be simply and quickly applied to molecular-assisted breeding of the Brassica campestris L.var. suitable for mechanical harvesting, and the breeding efficiency is greatly improved.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

The invention relates to the technical field of bioinformatics and crop breeding, and particularly discloses a method, a device and equipment for predicting the close planting yield of brassica napus by using hypocotyl length based on a whole genome selection technology. The method is characterized by comprising the following steps: acquiring whole genome sequencing data of brassica napus germplasm; inputting the whole genome sequencing data into a target model; outputting hypocotyl length data of the brassica napus under a close planting condition through the target model; and according to the hypocotyl length data of the brassica napus under the close planting condition, obtaining a brassica napus germplasm yield potential sorting result. According to the method, whole genome selection and key morphological characters (hypocotyl length) under specific stress (close planting conditions) are combined for the first time, high-yield varieties suitable for close planting cultivation can be efficiently and accurately screened out without on-site close planting tests in the early stage of rape breeding, and the breeding efficiency is remarkably improved.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The application discloses a method for cultivating and obtaining gene edited Punica granatum by a two-step somatic embryogenesis technique, which comprises the following steps: material obtaining, obtaining of embryogenic callus, preparation of Agrobacterium tumefaciens liquid, pre-culturing of callus, infection, co-culturing, washing, selection culture, subculture and ex vitro rooting, etc. The application firstly takes Punica granatum stem segments as explants to establish a Punica granatum gene editing plant system and obtain Punica granatum gene editing plants, avoids the separation of offspring traits when taking Punica granatum seed hypocotyls as explants, and lays a good foundation for subsequent researches on Punica granatum gene function and fine variety cultivation; in addition, the application further refines the somatic embryogenesis conditions under the selection pressure of Kan and TMT on the basis of the established Punica granatum somatic embryogenesis, directly transforms embryogenic cells by using the bipolar characteristics of somatic embryos, and thus complete and stable Punica granatum gene editing plants are obtained, and the transformation rate is high.

The application discloses an Arabidopsis beta-ketoacyl-ACP reductase gene promoter and application thereof. The promoter has the nucleotide sequence shown in SEQ ID No. 1, and controls the Arabidopsis beta-ketoacyl-ACP reductase gene to be specifically expressed in the hypocotyl and root of the Arabidopsis seedling, the leaf and pistil stigma of the seedling, and the fungal infection site of the seedling leaf after inoculation of powdery mildew (Erysiphe cichoracearum, UCSC1). G. cichoracerum The application provides a new tissue-specific and white-powder-fungus-infection-specifically-induced expression promoter, and provides a new regulating element for improving the genetic engineering breeding of the plant resistance to the white-powder fungus.

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

The invention relates to a construction method of an efficient regeneration system of idesia polycarpa, which comprises the following steps: soaking idesia polycarpa seeds in 3% gibberellic acid diluted by 1000 times for 3 days, sterilizing, inoculating to a germination culture medium of 1 / 2MS, 1.0 mg / L 6-benzylamino adenine (6-BA) and 0.5 mg / L 3-indolebutyric acid (IBA), and culturing for 25 days to obtain seedlings; a hypocotyledonary axis at the position 2 cm below the cotyledon of the seedling is obliquely cut and inoculated to a culture medium containing MS, 1.0 mg / L of thidiazuron (TDZ) and 0.5 mg / L of IBA, and calluses are induced after 30 days; inoculating the calluses to a culture medium containing MS, 2.0 mg / L TDZ and 0.05 mg / L IBA, and differentiating regenerated buds after 30 days; after seedling strengthening and rooting culture, the seedlings are transplanted to a sterilization matrix with the ratio of nutrient soil to vermiculite to perlite being 4: 1: 1. The differentiation rate of regenerated seedlings reaches 64.82% and is remarkably higher than that in the prior art, the seed germination rate and the transplanting survival rate are high, and an idesia polycarpa regeneration system can be efficiently constructed.

This invention discloses the application of the rice LOC_Os01g67410 gene in plant response to high ambient temperature. PLT5 It is a negative regulatory factor for deep-seeded rice seedlings. The length of internodes and mesocotyls during the seedling stage... plts The mutant showed no significant difference in germination speed and germination rate under shallow sowing conditions compared to the wild type, but under deep sowing conditions, it germinated faster and had a higher germination rate than the wild type. Furthermore, although a warm environment of 32℃ promoted earlier and faster germination time for both the wild type and the mutant, the germination rate of the wild type decreased significantly at 32℃. plts The mutant was still able to maintain a high germination rate. plts The mutant's tolerance to deep sowing and strong emergence performance suggest its important application value in dry direct sowing, especially in high-temperature summer direct sowing production. This can be achieved by finding mutants in natural populations or cultivars. PLTs The superior haplotype will be beneficial for breeding rice varieties suitable for deep sowing.

The invention discloses a method for establishing a gleditsia sinensis tissue culture regeneration system, and belongs to the technical field of plant tissue culture. Comprising the following steps: (1) inoculating explants from wild gleditsia sinensis seeds into an adventitious bud induction culture medium, and performing induction culture to generate adventitious buds; and (2) cutting off the adventitious bud from the base, inserting the cut end into a root induction culture medium, and inducing rooting. According to the method, hypocotyls and cotyledons of gleditsia sinensis aseptic seedlings are used as explants, and the influence of different plant growth regulator combinations, browning inhibitors and the like on the regeneration efficiency is discussed. According to the method, an efficient gleditsia sinensis regeneration system is successfully established, and a theoretical basis and a technical support are provided for high-quality seedling breeding and genetic engineering genetic improvement.

The invention discloses a breeding method of a potherb mustard variety suitable for mechanical harvesting, which comprises the following steps: taking a cytoplasmic male sterile line 08-5-6A of a potherb mustard local variety straw mustard as a female parent and taking a Shaoxing potherb mustard local variety brassica juncea selfing line 18-3-21-29-3-1-3 as a male parent for hybrid seed production to breed a new potherb mustard variety Yongxue No.15 with an upright and compact plant type. The Yongxue No.15 bred by the breeding method is upright and compact in plant type, bolting-resistant, 2-3cm in overground and hypocotyl length and suitable for mechanical harvesting, the plant height is more than 50cm, the weight of a single plant is more than 1kg, the yield per mu is more than 6000kg, and wide planting of the bred Yongxue No.15 has important significance for promoting industrial science and technology progress and high-quality development.

The invention relates to an oximido triazole compound as well as a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The oximido triazole compound disclosed by the invention has a vigorous growth control effect, can effectively control vigorous growth of crops such as peanuts, rice and soybeans, improves the yield and quality of the crops, and has the characteristics of strong vigorous growth control effect, small application amount, safety to the crops and the like. Experimental results show that the oximido triazole compound has the effects of inhibiting the plant height of rice and promoting the root growth of rice, has the effects of inhibiting the hypocotyl length of cucumber and tomato seedlings, and has the effects of inhibiting the vigorous growth of peanut plants and improving the chlorophyll content, the fresh fruit weight and the fruit number of peanuts.

The utility model relates to the technical field of automatic grafting, and discloses a seedling grafting clamp limiting, pinching and releasing device which comprises a pneumatic finger and grafting clamping jaws driven by the pneumatic finger, and a limiting plate is arranged between the grafting clamping jaws. The limiting plate is used for abutting against one side of the butterfly clamp to prevent the butterfly clamp from retreating when clamping the ear seedling hypocotyl and the stock hypocotyl. Staggered push plates are symmetrically arranged in the vertical direction of the grafting clamping jaw up and down, and the staggered push plates are used for stretching out to force the skew stock or ear seedling hypocotyl to restore to a preset grafting position. According to the utility model, through the arranged limiting plate, when the butterfly clip is pinched or released, the butterfly clip is prevented from retreating, so that the accurate position of the butterfly clip is ensured; meanwhile, a pair of staggered push plates are arranged in the vertical direction of the grafting clamping jaw, the seedlings are bound up to extend forwards to be close to the stock and the hypocotyls of the ear seedlings, and the inclined stock or the ear seedlings are pushed back to the correct position.

The invention relates to a method for increasing plant growth by administering phytoinitiator mRNAs obtained from seedling chloroplasts. The mRNAs can either be translated directly into chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts as proteins. In both cases, the resulting proteins lead to the activation of hormonal signaling pathways, increased protein synthesis, and a significant increase in root biomass, shoot number, and hypocotyl growth. The invention further comprises formulations and application systems such as granules, sprays, or injections that enable efficient uptake and action of the mRNAs.

The invention relates to a plant grafting technology in agricultural horticulture, in particular to a bacterial liquid preparation for promoting healing of grafting unions of muskmelons and pumpkins and application of the bacterial liquid preparation, and provides a bacterial liquid preparation which is simple in operation method, outstanding in application effect and capable of promoting healing of grafting unions of the muskmelons and the pumpkins according to physiological and molecular mechanisms of CmGH9B3 infection liquid in grafting healing of the muskmelons and the pumpkins. The bacterial liquid preparation can significantly improve the grafting healing efficiency of muskmelon and pumpkin and the quality of grafted seedlings. In the attaching grafting process of the muskmelon and the pumpkin, the muskmelon scion hypocotyl is soaked in a bacterial liquid preparation to be infected for 30 min, the muskmelon scion hypocotyl and the pumpkin rootstock are fixed through a grafting clamp, callus formation at a grafting opening and connection between the scion and a vascular bundle bridge of the rootstock can be promoted, and then healing of the grafting opening of the muskmelon and the pumpkin is accelerated; the grafting healing rate and survival rate are improved, and the grafted seedling quality is guaranteed.

The invention relates to the technical field of plant genetic engineering, in particular to application of a plant HSF family transcription factor HSFA2B in regulation and control of temperature response growth of plant mesocotyl. The invention provides application of a plant HSF family transcription factor HSFA2B in regulating and controlling temperature response growth of mesocotyl in a plant seedling stage or regulating and controlling direct seeding emergence of plants at high environment temperature. The invention finds that HSFA2B has an important function in rice mesocotyl response temperature growth regulation and control. The mesocotyl of the mutant with the HSFA2B knocked out is obviously longer than that of the wild type at the proper environment temperature, but the mesocotyl of the mutant with the HSFA2B knocked out is not obviously different from that of the wild type at the high environment temperature, and the response sensitivity to the high environment temperature is reduced. The discovery of the new function of the HSFA2B is of great significance to the breeding and direct seeding production of rice direct seeding varieties.

The invention relates to a method for increasing plant growth by delivering phyto-initiator mRNAs derived from chloroplasts of seedlings. The mRNAs may either be translated directly in chloroplasts or first translated in the cytosol and subsequently imported into the chloroplasts in the form of proteins. In both cases, the resulting proteins activate hormonal signalling pathways, increased protein synthesis, and bring about a significant enhancement of root biomass, shoot number and hypocotyl growth. The invention also relates to formulations and application systems such as granules, sprays or injections which enable efficient uptake and activity of the mRNAs.

The invention belongs to the technical field of gene engineering, and particularly relates to a method for knocking out a brassica napus BnaFAH gene by utilizing a CRISPR-Cas9 system and application. Two copies of the brassica napus BnaFAH gene are knocked out at a fixed point by using a CRISPR-Cas9 system, two target fragments are selected from conserved regions of fourth and sixth exons of the two copies of the brassica napus BnaFAH gene, BnaFAH-Cas9 plant expression vectors are respectively constructed, the BnaFAH-Cas9 plant expression vectors are introduced into brassica napus hypocotyl callus through an agrobacterium infection method, and seedlings are regenerated. Two copies of the BnaFAH gene are subjected to double-strand shearing under the combined action of two sgRNAs and Cas9 nuclease, random deletion on a target gene segment is finally realized through a cell self-repairing function, gene knockout is completed, a mutant plant is obtained, the mutant plant obtained after the BnaFAH gene is knocked out is verified to enhance the resistance to plutella xylostella, and the mutant plant has a good application prospect. Therefore, the gene is a key gene for regulating and controlling the insect resistance of the brassica napus, and has important significance for breeding new insect-resistant varieties of the brassica napus.

The invention discloses an application of a plant AP2 / EREBP family transcription factor PLT1 gene. PLT1 is a negative regulation factor for deep-sowing seedling growing of rice. The seedling emergence speed and the seedling emergence rate of the plts mutant with the internode length and the mesocotyl length in the seedling stage are not obviously different from those of the wild type under the shallow sowing condition, but the seedling emergence speed and the seedling emergence rate of the plts mutant are higher than those of the wild type under the deep sowing condition; meanwhile, although the warm environment at 32 DEG C promotes the emergence time of the wild type and the mutant to be advanced and the emergence speed to be accelerated, when the emergence rate of the wild type is greatly reduced at 32 DEG C, the plts mutant can still keep a relatively high emergence rate. The deep sowing resistance and strong seedling emergence performance of the plts mutant prompt that the plts mutant has important application value in dry direct seeding, especially in dry direct seeding production at high temperature in summer. The excellent haplotype of PLTs is found in a natural population or a cultivated variety, so that the cultivation of a rice variety suitable for deep sowing is facilitated.

The invention relates to the technical field of biology, in particular to a Chinese cabbage hypocotyl length related molecular marker and application thereof. The invention firstly provides an application of the molecular marker related to the length of the hypocotyl of the Chinese cabbage or a substance for detecting the molecular marker related to the length of the hypocotyl of the Chinese cabbage in identification or auxiliary identification of the length of the hypocotyl of the Chinese cabbage. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to a difference between sequence fragments of 49-420 sites of SEQ ID No.1 and sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to the difference between the sequence fragments of 49-420 sites of SEQ ID No.1 and the sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage can be used for identifying or assisting in identifying the length of the hypocotyl of the Chinese cabbage in a seedling stage, and the breeding process of a new variety of the long-hypocotyl Chinese cabbage is accelerated.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a method for establishing a tissue culture system of Rheum tanguticum. The culture system comprises taking leaves, petioles and hypocotyls of Rheum tanguticum as explants, using MS medium, and sequentially carrying out induction of callus, adventitious buds and adventitious roots, and through adding different concentrations and different combinations of hormones, it is determined that the leaves and petioles are induced into callus, and then the propagation of Rheum tanguticum is realized in the form of artificial seeds, the hypocotyls can be directly induced into adventitious buds, or can be induced into callus first, then into adventitious buds, and finally into adventitious roots, so as to realize the cultivation of Rheum tanguticum. Through the above method, the application of Rheum tanguticum tissue is widened, so that each part can be used for plant regeneration culture, and the regenerated plant of Rheum tanguticum is successfully obtained, and the tissue culture system of Rheum tanguticum is formed, which lays a foundation for the artificial rapid propagation and genetic improvement of Rheum tanguticum.

The invention discloses application of a keratin related protein gene GbKAP related to cotton fiber quality characters, and belongs to the field of biotechnology application. The growth vigor and plant height of root cells, main roots, hypocotyls and rosette leaves of an arabidopsis thaliana plant for heterologous expression of the cotton keratin related protein gene GbKAP are superior to those of a control group. The GbKAP plays an important role and has an application prospect in promoting cell elongation, improving the quality character of cotton fibers and cultivating new varieties of high-quality cotton fibers.