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Common head cabbage tissue culture method

A technology for cabbage and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of reduced reproduction coefficient, poor growth, weak plants, etc., and achieves high proliferation rate and fewer vitrified seedlings. , the effect of strong growth

Inactive Publication Date: 2012-12-05
XINGTAI CITY VEGETABLE SEED CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the current common techniques for tissue culture of head cabbage and cultivating tissue culture seedlings on the existing head cabbage tissue culture medium for a long time will easily cause the tissue culture seedlings to gradually decline and lose their morphogenetic ability, showing weak plants, poor growth, and poor proliferation. rate drop
Even some tissue culture seedlings appear vitrification, and the petioles and leaves of the tender stems appear translucent and water-soaked, which makes the growth of test tube seedlings slow, and because the tender stems are not easy to induce rooting, the reproduction coefficient is greatly reduced. There is no wax on the surface of the stems and leaves of the seedlings, the level of polar compounds in the body is high, the water holding capacity of the cells is poor, and the transpiration of the plants is strong, so normal transplanting cannot be performed
For a long time, it is not conducive to the subculture, long-term preservation, rooting and transplanting of cabbage tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for tissue culture of head cabbage, comprising the following process steps:

[0030] A. Cultivation of aseptic seedlings: Disinfect the high-quality head cabbage seeds selected after removing impurities, inoculate the sterilized seeds in MS medium for dark culture, and make aseptic seedlings, inoculate 8 seeds according to 48ml medium The inoculation amount of seeds is inoculated, the cultivation temperature is 22°C, the cultivation humidity is 70%, and the cultivation period is 9-12 days;

[0031] B. Induction culture: cut the cotyledons of the aseptic seedlings after dark culture into 4mm×4mm pieces, cut the hypocotyls into 3.6mm pieces, inoculate them on the induction differentiation medium for induction culture, and observe the explants during the induction culture process Changes and induction of adventitious buds; the composition of the induction differentiation medium is:

[0032] MS+6-BA 2.0mg / L, NAA 0.1mg / L, sucrose 25g / L, agar 6.5g / L, pH=5.8;

[003...

Embodiment 2

[0045] A method for tissue culture of head cabbage, comprising the following process steps:

[0046] A. Cultivation of aseptic seedlings: Disinfect the high-quality head cabbage seeds selected after removing impurities, inoculate the sterilized seeds in MS medium for dark culture, and make aseptic seedlings, inoculate 10 seeds according to 52ml medium The inoculation amount of seeds is inoculated, the cultivation temperature is 26°C, the cultivation humidity is 80%, and the cultivation period is 9-12 days;

[0047]B, induction culture: the cotyledons of aseptic seedlings are cut into 4mm * 4mm piece, hypocotyl is cut into 4.2mm, inoculate on induction differentiation medium and carry out induction culture, observe the change of explant in induction culture process and The induction situation of adventitious buds; the composition of induction differentiation medium is:

[0048] MS+6-BA 3.0mg / L, NAA 0.2mg / L, sucrose 30g / L, agar 7g / L, pH=5.8;

[0049] The culture conditions for...

Embodiment 3

[0062] A method for tissue culture of head cabbage, comprising the following process steps:

[0063] A, the cultivation of aseptic seedlings: the high-quality head cabbage seeds selected after removing impurities are sterilized, the seeds after sterilizing are inoculated in MS medium and carry out dark culture to make aseptic seedlings, according to 50ml medium inoculation 8- The inoculum amount of 10 seeds is inoculated, the cultivation temperature is 25°C, the cultivation humidity is 70%-80%, and the cultivation period is 9-12 days;

[0064] B, induction culture: cut the cotyledons of the aseptic seedlings after dark culture into 4mm×4mm pieces, cut the hypocotyls into 4mm, inoculate them on the induction differentiation medium for induction culture, and observe the explants during the induction culture process Changes and induction of adventitious buds; the composition of the induction differentiation medium is:

[0065] MS+6-BA 2.5mg / L, NAA 0.15mg / L, sucrose 28g / L, agar 7...

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Abstract

The invention belongs to tissue culture, in particular to a common head cabbage tissue culture method. The method comprises the process steps of aseptic seedling culture, induction culture, multiplication culture, rooting culture, regeneration seedling transplanting and the like. The method provided by the invention solves the problems that in the prior art, the subculture, the long-time storage and the rooting transplanting and utilization of common head cabbage tissue culture seedlings cannot be favorably realized, and the like. When being adopted, the method has the advantages that callus tissue can grow on cotyledons on a common head cabbage induction culture medium, hypocotyls can be directly differentiated into adventitious buds, the tissue culture seedling multiplication times is 3.8 to 4.6, the growth condition of the tissue culture seedlings is strong, vitrification seedlings are few, the multiplication rate is high, and the rooting rate of the tissue culture seedlings can reach 100 percent, and the like.

Description

technical field [0001] The invention belongs to tissue culture, in particular to a method for tissue culture of head cabbage. Background technique [0002] Heading cabbage seeds are expensive and have a long seedling age. In order to improve land utilization, the method of raising seedlings first and then transplanting is often used in practice. Since the seedlings of head cabbage are taproots and have few fibrous roots, it is not conducive to the absorption of nutrients and water by the roots. After transplanting and planting, the growth is slow, they are not resistant to waterlogging and root diseases, and the yield is not high. In recent years, with the rapid development of agricultural mechanization, precision seeding, plug seedling cultivation, and transplanting machine colonization have been introduced into cabbage cultivation. How to eliminate the above-mentioned cultivation defects and increase the yield of cabbage has become an urgent technology to be solved. probl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 申志彬申志恒王僧虎石晓云
Owner XINGTAI CITY VEGETABLE SEED CO
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