The invention provides a catalpa tissue culture rapid propagation method, and relates to the technical field of plant tissue culture. A catalpa tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The primary culture medium and the proliferation culture medium are Qj culture mediums: N6+6-BA 1.25 mg/L+IBA 0.07 mg/L+ agar 6.5 g/L+ sucrose 30g/L, and the rooting culture medium selects a Qg culture medium: 1/2MS+6-BA0.01mg/L+IBA 0.5mg/L+ agar 6.5g/L+ sucrose 15g/L. The method catalpa tissue culture rapid propagation method using the catalpa tissue culture medium includes the following steps: 1) obtaining sterile seedlings, to be more specific, selecting new shoots, treating the lengths, cleaning, disinfecting, sterilizing, cutting, inoculating into the Qj mediums, and cultivating to obtain sterile seedlings, 2) performing proliferation culture, to be more specific, treating the lengths of the sterile seedlings, transferringthe sterile seedlings to the Qj mediums, and cultivating to obtain subculture seedlings; 3) performing rooting culture, to be more specific, treating the subculture seedlings, inoculating the subculture seedlings into the Qg culture medium, and cultivating to obtain rooted seedlings; and 4) hardening seedlings and transplanting, to be more specific, transferring the rooted seedlings to a greenhouse for hardening the seedlings, transplanting the seedlings to an aperture disk, and transplanting after cultivation. The catalpa tissue culture rapid propagation method has the beneficial effects that effective multiplication rate of rooting culture is high, seedling rate is high, and factory production can be facilitated.