120 results about "Multiplication rate" patented technology

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial system are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplication rate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.

The invention provides to a method for tissue culturing and quick propagation of sugarcane with an intermittent immersion bioreactor, which is characterized in that: the method performs propagation culture and rooting culture by transplanting detoxicated sugarcane plants, serving as materials, cultured by generations from induced sprouts of sugarcane stem tip tissues into the intermittent immersion bioreactor. In the intermittent immersion bioreactor, a culture temperature is 29 DEG C; the illumination intensity is 1,500 Lx and the illumination time is 16 hours; an intermittent culture condition is to culture for 3min every 3h and to root for 3min every 6h; the first generation can be propagated to 40 times; after rooting is completed, a proper hardening plantlet treatment is performed; and plantlets are transplanted and the survival rate is about 85 percent. The set of system is high in automation degree, has a much higher multiplication rate than that of a traditional tissue culture method, saves labour cost and increases economic benefits.

The invention provides a method for efficiently preparing an NK cell, which can improve the multiplication rate and the purity of the NK cell through combination of stimulation effects of cell factors and feeder cells. The method is mainly characterized by comprising the steps as follows: NCR3LG1 and m IL-15 are transfected to a K562 cell simultaneously, m IL-15 can be used for adjusting activation and multiplication of the NK cell, NCR3LG1 serving as a ligand of NKp30 which is one of main activated receptors on the surface of the NK cell can effectively stimulate activation of the NK cell, and NCR3LG1 and m IL-15 have a synergistic effect; and PBMC(peripheral blood mononuclear cells) can be multiplied over 500 times in 21 cultivation days through stimulation of factors of freely added IL-2, IL-21 and the like, and a proportion of CD3-CD56+NK cell exceeds 70%; and up to now, a research report that NCR3LG1 and m IL-15 are transfected to the feeder cells simultaneously and jointly stimulate and activate the NK cell in combination of free cell factors is absent. The invention firstly provides a method for jointly cultivating and preparing the NK cell in combination of the feeder cells and the free factors.

The present invention relates to a culture method for the detoxication and the tissue culture of the bulb of Fritillaria thunbergii, which belongs to the technical field of breeding the plant seedling and is specially used for breeding the detoxic seedling of Fritillaria thunbergii and the detection of the virus of Fritillaria thunbergii. The method comprises the steps such as the formulation of the culture medium, the culture of the detoxication tissue culture bulb, the detection of the virus ELISA, etc. The present invention has good detoxication effect, high sensitivity of the virus detection, and can guarantee that the detocication daughter bulb is free from virus, thereby effectively controlling the occurrence and the broadcasting of the virus disease; the multiplication of the bulb is fast, and the monthly multiplication rate can reach 4.5 times, thereby being applicable to the factory seedling raising, greatly saving the breeding land of the traditional method and obviously reducing the production cost of Fritillaria thunbergii; the present invention can be applied to the purification and rejuvenation and the development of the species of Fritillaria thunbergii.

The invention relates to the technical field of agriculture and discloses a microbial type mineral fertilizer and a preparation method of the microbial type mineral fertilizer. The microbial type mineral fertilizer disclosed by the invention comprises the following components by weight: 35-70% of medical stone powder, 10-25% of microbial strains and 20-30% of humic acid or humate or mixture of the humic acid and the humate. According to the invention, a novel microbial type mineral fertilizer is prepared by taking microbial strains (such as bacillus subtilis, bacillus jelly and the like), a mineral fertilizer and humic acid (humate) as raw materials, and the fertilizer can accelerate the multiplication rate of the microbial strains, realize slow release and controlled release of secondary and trace elements and regulate soil nutrients, thereby reducing the incidence rate of diseases of crops and improving the yield of crops.

A secondary Booth-code-based large number multiplier belongs to the integrated circuit design technology field of the public key encryption algorithm. The present invention uses the linear transformation formula B=8a+b to do a secondary coding to the Booth 64 algorithm results generated by the partial product. The multiplier based on the secondary Booth 64code is divided into 3-level pipelining architectures. The first level architecture precomputes the multiplicands of three times with a carry look-ahead adder and at the same time does secondary Booth coding to aj with a weight of 81 and bj with a weight of 80. The second level architecture, consisting of two identical partial products selection and compression arrays, simplifies the partial products of aj and bi respectively. The third level architecture adds the partial products of the second level by an adder. The present invention improves the multiplication rate performance and can be applied to high performance RSA and ECC chips as well as large scale PKI system of servers.

The invention discloses an electrode of a capacitor and a preparation method thereof, and relates to a mixed super capacitor electrode based on polyaniline / directional carbon nanotube composite material and a preparation method thereof. The electrode is characterized by comprising a conductive matrix material and a polyaniline / directional carbon nanotube composite material; and the polyaniline / directional carbon nanotube composite material directly grows on one side or both sides of the conductive matrix material. In the preparation process, by selecting reaction gas and conductive matrix material and controlling chemical gas phase deposition technology, the directional carbon nanotube directly grows on a conductive matrix; then, electrochemical deposition of the directional carbon nanotube is carried out in vitriol solution of prepared hydrochloric acid aniline; and a polyaniline / directional carbon nanotube composite electrode with large power density, good multiplication rate performance, high energy density and long service life for a super capacitor is prepared. The invention has the characteristics of simple preparation process, low cost, easy large-scale production and the like.

The invention provides a serum-free culture medium for umbilical cord mesenchymal stem cells. The serum-free culture medium is characterized by comprising a DMEM low-sugar culture medium, transferrin, serum albumin, insulin, platelet-derived growth factors, epidermal growth factors, transforming growth factors, beta-mercaptoethanol, dihydromyricetin and catechin. The invention belongs to the technical field of stem cell culture. The serum-free culture medium for umbilical cord mesenchymal stem cells, provided by the invention, can obviously promote the adherence performance and multiplication rate of the umbilical cord mesenchymal stem cells, is beneficial to the multiplication of the umbilical cord mesenchymal stem cells and the maintenance of features of the stem cells, is excellent in adipogenesis and osteogenic induction differentiation potential, is relatively simple in component of the culture medium and is relatively low in cost.

The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention belongs to the technical field of microalgae and in particular relates to a high-density continuous culture method and device for the microalgae. The device comprises a photobioreactor circulating unit, a nutrient solution replenishing unit, a ventilating carbon-replenishing unit and a microalgae collecting unit. The method using the device comprises the following steps: (1) inoculating the microalgae; (2) multiplying the microalgae; (3) collecting the microalgae; (4) replenishing a culture medium; (5) repeating the steps (2) to (4). According to the method, the multiplication rate of microalgae cells is adjusted and controlled through timely collecting and replenishing processes, so that the logarithm growth period of the microalgae cells in the photobioreactor circulating unit is longer, the high-density continuous culture of the microalgae is achieved, a microalgae product with higher yield can be obtained, and the yield can be increased by more than 30% in comparison with a conventional batch culture method in the microalgae industry. According to the device, a control valve can adopt a solenoid valve and large-scale centralized control can be achieved.

The invention relates to a low-temperature treatment method of A / O / MBBR (Anaerobic / Oxic / Moving Bed Biofilm Reactor) municipal sewage. The low-temperature treatment method of A / O / MBBR municipal sewage is used for solving the problem of low nitrification efficiency of the existing sewage treatment method in north because autotrophic nitrifying bacteria are difficult to accumulate in a low-temperature system due to low multiplication rate. The low-temperature treatment method of A / O / MBBR municipal sewage is realized through the following steps of: 1, guiding the municipal sewage to a water inlet pool through primary treatment; 2, guiding the municipal sewage to an anaerobic pool; 3, guiding the municipal sewage to an aerobic I section; 4, guiding the municipal sewage to an MBBR system from the aerobic I section; 5, guiding the municipal sewage to a secondary sedimentation tank from the MBBR system; and 6, guiding the municipal sewage to a sludge hydrolyzing pool from the secondary sedimentation tank. The low-temperature treatment method of A / O / MBBR municipal sewage can be used for the field of winter sewage treatment engineering in northern cities.

The invention relates to a maintenance time prediction method based on a virtual maintenance simulation process and belongs to the technical field of design of auxiliary products of the virtual reality technology. To overcome the defects that a traditional maintenance time prediction method is tedious in calculation and low in visualization degree and manpower and materials are wasted, statistics is performed on maintenance action design simulation rules with the method to determine the compensation time of each maintenance action, then, the maintenance process is simulated through the virtual reality technology, after the simulation, statistics is performed on the number of the maintenance actions in the simulation process, and the maintenance time is predicted according to the compensation time. According to the compensation time, corresponding compensation is performed on the differential value or the multiplication rate between a single actual maintenance action and the virtual operation. The method avoids tediousness and uncertainty of a traditional method in the calculation process, the maintenance time of a new product is predicted objectively and systematically in the early stage of product design through a product model machine, and auxiliary support is provided for the design scheme of the maintenance time of the product.

The invention belongs to tissue culture, in particular to a common head cabbage tissue culture method. The method comprises the process steps of aseptic seedling culture, induction culture, multiplication culture, rooting culture, regeneration seedling transplanting and the like. The method provided by the invention solves the problems that in the prior art, the subculture, the long-time storage and the rooting transplanting and utilization of common head cabbage tissue culture seedlings cannot be favorably realized, and the like. When being adopted, the method has the advantages that callus tissue can grow on cotyledons on a common head cabbage induction culture medium, hypocotyls can be directly differentiated into adventitious buds, the tissue culture seedling multiplication times is 3.8 to 4.6, the growth condition of the tissue culture seedlings is strong, vitrification seedlings are few, the multiplication rate is high, and the rooting rate of the tissue culture seedlings can reach 100 percent, and the like.

The invention discloses high-temperature and high-humidity resistant saccharomyces boulardii and application thereof, and belongs to the field of microorganisms. The preservation number of the high-temperature and high-humidity resistant saccharomyces boulardii disclosed by the invention is CGMCC No.10381; the multiplication rate and maximal biomass (namely final density of thallus) of saccharomyces boulardii strains are significantly higher than those of similar saccharomyces cerevisiae strains; the saccharomyces boulardii has high heat-resistant and moisture-resistant capacities; the saccharomyces boulardii disclosed by the invention can be used as a feed additive; and relatively high activity is stored in the high-temperature and high-humidity treatment processes. According to a saccharomyces boulardii capsule, daily gain, immune organ index, disease resistance, oxidation resistance and immune globulin of broiler chicken can be improved; the saccharomyces boulardii is free of a toxic or side effect on beasts and birds, wide in source and low in price, and can be used as a feed additive instead of antibiotics.

The invention discloses a skin stretching and fixing frame which comprises a bracket and a skin stretching and fixing component, wherein the bracket comprises two supporting rods, and the skin stretching and fixing component comprises a fixing rod I and a fixing rod II; the fixing rod I and / or the fixing rod II are / is in sliding fit with the two supporting rods; and fixing buckles which are in one-to-one correspondence are arranged on the fixing rod I and the fixing rod II. When the skin stretching and fixing frame is used, the skin of an animal model is stretched through the skin fixing buckles, the limbs of the animal model are bound on the bracket through ropes, and the interval between the fixing rod I and the fixing rod II is adjusted by sliding the fixing rod I and / or the fixing rod II, thereby changing the stretching force born by the skin of the animal model; and when the interval reaches the required degree, the fixing rod I and / or the fixing rod II are locked and fixed to keep the skin stretching state stable. The whole device has the advantages of simple structure, easy manufacture and convenient operation, and is suitable for researching and observing the change of biological behaviors such as multiplication rates, growth states and the like of different cells under the condition of different stretching force and pathological and physiological processes and related mechanisms in the healing process of the wound surface of the animal model.

The invention discloses a video multi-speed playing method and system based on voice. When a video file is preprocessed, the method comprises the steps that A, voice information in the video file is read; B, analyzing the voice information, decomposing the video file into a plurality of sub-videos according to the speed of the voice information, and obtaining the speed multiplication rate of eachsub-video segment based on the corresponding voice information; C, forming a speed multiplication description file by utilizing the speed multiplication rate corresponding to each sub video segment; when the video file is played, the method comprises the following steps: step D, loading and analyzing a speed multiplication description file; and step E, when the video file is played to a certain sub-video segment, playing the video file according to the speed rate corresponding to the sub-video segment in the speed description file. According to the method, the speed multiplication rate can beautomatically calculated through the voice in the video, so that a user can watch the complete video at the most appropriate speed multiplication rate which is intelligently changed, the speed multiplication rate can be automatically and intelligently adjusted in the video speed multiplication playing process, manual adjustment of the user is not needed, and the user experience is good.

The invention discloses a tissue culture propagation method for clematis lanuginosa. The excellent effects of high multiplication rate, stable hereditary feature, high reproduction coefficient and vigorous test-tube plantlet growth are achieved through the steps of primary culture, multiplication culture, rooting culture, seedling hardening and transplanting mainly, the germinating rate of the callus of the clematis lanuginosa is up to 98%, the multiplication multiple is up to 5.7 times, the rooting rate is up to 100%, and the final survival rate is up to 96%.

The invention discloses a quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis, which comprises the following steps of the selection and disinfection of explants, induction culture, multiplication culture, root induction culture and transplantation rooting. In the tissue culture propagation method for the narcissus tazetta var. chinensis, the complete technical process from the selection of the explants to the transplantation of the explants is accomplished, and bulbs subjected to the multiplication culture are rooted and induced within short time and are transplanted to a perlite substrate for rooting directly, so that test tube seedlings of the narcissus tazetta var. chinensis are not needed to be rooted in vitro; and the steps of induction rooting and transplantation are simplified, the production period is shortened, the cost is reduced, and the survival rate of the transplantation reaches over 92 percent. The method is high in pertinence, simple and easy and high in multiplication rate, produced seed bulbs are high in consistency and few in plant diseases and insect pests, the cost is saved, and the requirements for the industrial production of the narcissus tazetta var. chinensis seedlings can be met.

The invention provides a windproof micro-pore pipeline spraying system for microbial sand stabilization and a using method thereof. The integral structure of the windproof spraying system is composedof longitudinal and horizontal micro-pore pipelines, windproof covering films and curved nails. According to the windproof micro-pore pipeline spraying system and the using method thereof, a microbialbacteria solution and a the nutrient solution additive are sprayed into a soil body through the longitudinal and horizontal micro-pore pipelines, the reinforced soil body is prevented from being blown away by large air through the wind-proof covering films, so that the overall stability of the reinforced soil body is improved; and the windproof micro-pore pipeline spraying system has the advantages that the multiplication rate of microorganism in the soil body needing to be reinforced can be guaranteed, a relatively closed environment is maintained so that the reinforcement reaction can be carried out continuously, and a certain strength and the certain reinforcing effect are obtained. The windproof micro-pore pipeline spraying system for microbial sand stabilization and the using methodthereof can be used in microbial sand stabilization and wind prevention projects and can also be used in desert control projects.

Provided is a power supply circuit generating a desired voltage by voltage multiplication, and satisfying both a demand to reduce current consumption and a demand to enable operation with a low power voltage at the same time. A power supply circuit of the present invention includes: a voltage generating circuit for generating internal voltages VI1 and VI2 from a power supply voltage VDD; a voltage step-up / down circuit for generating voltages VO1 to VO3 each having a different level by multiplying the internal voltages VI1 and VI12; and a voltage comparison circuit for comparing the voltage VO2 with the power supply voltage VDD. The voltage generating circuit is configured to select one of the internal voltages VI1 and VI2 according to an output of the voltage comparison circuit. Additionally, a voltage multiplication rate of the voltage multiplication circuit is switched according to the output of the voltage comparison circuit.

The invention provides ringlike RNA circ-CCNY and application thereof. The nucleotide sequence of the circ-CCNY gene is as shown in SEQ ID NO: 1; the expression level of the ringlike RNA circ-CCNY is obviously reduced by detecting the expression condition of the circ-CCNY gene in a liver cancer patient; and the multiplication rate of the ringlike RNA circ-CCNY is obviously reduced by comparing liver cancer cells for expressing the RNA circ-CCNY gene with control liver cancer cells. The ringlike RNA circ-CCNY gene and an expression product thereof serve as markers of diagnosing liver cancer, so that diagnosis of the liver cancer is more accurate and quicker, and the ringlike RNA circ-CCNY gene as a target gene for preparing drugs capable of curing the liver cancer provides a new therapeutic target and therapy pathway for curing the liver cancer.

The invention discloses a method for determining a coaxial configuration micro-discharging threshold value. The method comprises the following steps of: determining an electronic movement locus and an electronic movement speed in a coaxial structure; converting probability of an emergence speed satisfying Maxwellian distribution into a joint probability density function of transit time; respectively carrying out maximum value and monotonicity processing to four classes of probability density functions to obtain a processed joint probability density function; taking an electron collision kinetic energy as an incidence electron energy of a secondary electron emission characteristic of a material to obtain a secondary electron multiplication function generated during electron collision under the transit time; constructing a steady-state equation which satisfies the electron number during micro discharging; judging whether a voltage generates micro discharging by solving an effective secondary electron multiplication rate in the steady-state equation; and gradually calculating an effective multiplication rate of a next voltage with a bisection method, wherein a corresponding voltage is a micro-discharging threshold value when the effective multiplication rate is 1. According to the method disclosed by the invention, an accurate micro-discharging threshold value can be obtained, and meanwhile, the threshold value can be quickly obtained.

The invention discloses tissue engineering skin with a layered structure and a preparation method of the tissue engineering skin, and relates to the technical field of tissue engineering skin. The tissue engineering skin with the layered structure comprises a collagen layer, a collagen layer embedded in human skin fibroblast, a first multilayer collagen layer, a collagen layer embedded in keratinocyte and a second multilayer collagen layer. According to the tissue engineering skin disclosed by the invention, a manner that channels are innovatively generated in tissues by a temporary material such as gelatin is adopted, and human umbilical vein endothelial cells are adhered to the inner parts of the channels, so that the formed blood vessels are better fit with human body blood vessels; thetransporting and distributing rate of the formed blood vessels is increased; cells have higher viability and high multiplication rate; the printed skin with the layered structure is adopted, so thatthe interaction between cells of the formed skin structure and the interaction of the skin tissues and epimatrix are more stable, and regeneration of the skin tissue is facilitated.

The invention provides an adipose-derived stem cell serum-free culture medium which comprises a DMEM low-sugar culture medium and further comprises transferrin, serum albumin, a platelet-derived growth factor, an epidermal growth factor, a transforming growth factor, beta-mercaptoethanol, dihydroarbutin, and catechin. The adipose-derived stem cell serum-free culture medium belongs to the technical field of cell culture. The adipose-derived stem cell serum-free culture medium can remarkably improve the adherence performance and multiplication rate of adipose-derived stem cells and facilitates multiplication and property keeping of the adipose-derived stem cells, the ingredients of the culture medium are simpler, and the cost is lower.

The invention provides a rooting culture method for pear test-tube plantlets and a culture medium, and belongs to the technical field of plant cell engineering. The rooting culture method comprises the following steps: carrying out multiplication culture, strong seedling culture, induced rooting culture and large-amount rooting culture in sequence based on pear test-tube plantlets; the induced rooting culture step comprises the following sub-steps: sequentially inoculating tissue culture seedlings obtained after the strong seedling culture into a rooting culture medium A and a rooting culture medium B to be cultured to obtain rooting seedlings; and the large-amount rooting culture step comprises the following sub-steps: inoculating the rooting seedlings into a rooting culture medium C to be cultured, wherein the rooting culture medium A is a culture medium obtained by adding IAA, BA and NAA into a 1 / 4QL culture medium, the rooting culture medium B is a 1 / 4QL culture medium and the rooting culture medium C is a liquid culture medium obtained by adding IBA into a 1 / 3MS culture medium. The invention further provides the culture medium used in the method. By using the rooting culture method provided by the invention, the rooted test-tube plantlets with the relatively high multiplication rate, rooting rate, average rooting quantity and the relatively long average root length can be obtained.

The invention discloses a fireproof paint, a preparation method and application thereof, and belongs to the field of fireproof material. The raw material components of the fireproof paint comprise sterile water, ammonium polyphosphate, melamine, dipentaerythritol, acetic styrene-acrylic emulsion, titanium dioxide, kaolin, wollastonite powder, vermiculite and graphite. According to the fireproof paint provided by the invention, the expanded multiplication rate of the fireproof paint can reach 15-30 times; a formed carbon layer is adamantine and compact and meanwhile will not fall down from a steel girder; and the fireproof time is increased. The fireproof paint solves the problem that the expanded multiplication rate of expansive type fireproof paints is low, and perfectly integrates chemical reaction expansion with physical expansion.

The invention provides a catalpa tissue culture rapid propagation method, and relates to the technical field of plant tissue culture. A catalpa tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The primary culture medium and the proliferation culture medium are Qj culture mediums: N6+6-BA 1.25 mg / L+IBA 0.07 mg / L+ agar 6.5 g / L+ sucrose 30g / L, and the rooting culture medium selects a Qg culture medium: 1 / 2MS+6-BA0.01mg / L+IBA 0.5mg / L+ agar 6.5g / L+ sucrose 15g / L. The method catalpa tissue culture rapid propagation method using the catalpa tissue culture medium includes the following steps: 1) obtaining sterile seedlings, to be more specific, selecting new shoots, treating the lengths, cleaning, disinfecting, sterilizing, cutting, inoculating into the Qj mediums, and cultivating to obtain sterile seedlings, 2) performing proliferation culture, to be more specific, treating the lengths of the sterile seedlings, transferringthe sterile seedlings to the Qj mediums, and cultivating to obtain subculture seedlings; 3) performing rooting culture, to be more specific, treating the subculture seedlings, inoculating the subculture seedlings into the Qg culture medium, and cultivating to obtain rooted seedlings; and 4) hardening seedlings and transplanting, to be more specific, transferring the rooted seedlings to a greenhouse for hardening the seedlings, transplanting the seedlings to an aperture disk, and transplanting after cultivation. The catalpa tissue culture rapid propagation method has the beneficial effects that effective multiplication rate of rooting culture is high, seedling rate is high, and factory production can be facilitated.

An anaerobic ammonia oxidation reactor device structurally comprises a water storage pond, a water feed pump, a water sealing device, a constant temperature device, a graphite electrode, an anaerobic ammonia oxidation reactor, a stirring paddle, a flat sheet membrane assembly, a direct current power supply, a stirring and rotating motor, an alternative current power supply, a membrane module water producing pump, a sample connection, a flat sheet membrane assembly flow channel, a sealing ring, a water producing pipe, a flat sheet membrane, a flow channel supporting plate and a PLC. A direct current electric field is arranged in the device, the existing characteristic of anaerobic ammonia oxidation bacteria is enhanced, the multiplication rate of the anaerobic ammonia oxidation bacteria is improved, the treatment efficiency is improved, management is convenient, adjustability is strong, and the anaerobic ammonia oxidation reactor device is resistant to impact load.

The invention discloses a method of a tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached mainly through the steps of primary culture, secondary culture, multiplication culture, rooting culture and hardening-plantlet transplantation during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 96%, the multiplication multiple is up to 4.35 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.