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120 results about "Multiplication rate" patented technology

Fast reproducing method for high quality seedling of dendrobium officinale

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial system are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplication rate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.
Owner:KUNMING UNIV OF SCI & TECH

Clonal tissue culture breeding method of Liquidambar formosana Hance

ActiveCN102823497ASolve the problem of long production cycleSolve the problem of breeding applicationsPlant tissue cultureHorticulture methodsAxillary budLand resources
The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.
Owner:GUANGDONG ACAD OF FORESTRY

Maintenance time prediction method based on virtual maintenance simulation process

The invention relates to a maintenance time prediction method based on a virtual maintenance simulation process and belongs to the technical field of design of auxiliary products of the virtual reality technology. To overcome the defects that a traditional maintenance time prediction method is tedious in calculation and low in visualization degree and manpower and materials are wasted, statistics is performed on maintenance action design simulation rules with the method to determine the compensation time of each maintenance action, then, the maintenance process is simulated through the virtual reality technology, after the simulation, statistics is performed on the number of the maintenance actions in the simulation process, and the maintenance time is predicted according to the compensation time. According to the compensation time, corresponding compensation is performed on the differential value or the multiplication rate between a single actual maintenance action and the virtual operation. The method avoids tediousness and uncertainty of a traditional method in the calculation process, the maintenance time of a new product is predicted objectively and systematically in the early stage of product design through a product model machine, and auxiliary support is provided for the design scheme of the maintenance time of the product.
Owner:BEIHANG UNIV

Skin stretching and fixing frame

InactiveCN101810514AStable skin stretchStable stretchSurgeryDiagnostic recording/measuringSkin stretchingMultiplication rate
The invention discloses a skin stretching and fixing frame which comprises a bracket and a skin stretching and fixing component, wherein the bracket comprises two supporting rods, and the skin stretching and fixing component comprises a fixing rod I and a fixing rod II; the fixing rod I and/or the fixing rod II are/is in sliding fit with the two supporting rods; and fixing buckles which are in one-to-one correspondence are arranged on the fixing rod I and the fixing rod II. When the skin stretching and fixing frame is used, the skin of an animal model is stretched through the skin fixing buckles, the limbs of the animal model are bound on the bracket through ropes, and the interval between the fixing rod I and the fixing rod II is adjusted by sliding the fixing rod I and/or the fixing rod II, thereby changing the stretching force born by the skin of the animal model; and when the interval reaches the required degree, the fixing rod I and/or the fixing rod II are locked and fixed to keep the skin stretching state stable. The whole device has the advantages of simple structure, easy manufacture and convenient operation, and is suitable for researching and observing the change of biological behaviors such as multiplication rates, growth states and the like of different cells under the condition of different stretching force and pathological and physiological processes and related mechanisms in the healing process of the wound surface of the animal model.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Video multi-speed playing method and system based on voice

The invention discloses a video multi-speed playing method and system based on voice. When a video file is preprocessed, the method comprises the steps that A, voice information in the video file is read; B, analyzing the voice information, decomposing the video file into a plurality of sub-videos according to the speed of the voice information, and obtaining the speed multiplication rate of eachsub-video segment based on the corresponding voice information; C, forming a speed multiplication description file by utilizing the speed multiplication rate corresponding to each sub video segment; when the video file is played, the method comprises the following steps: step D, loading and analyzing a speed multiplication description file; and step E, when the video file is played to a certain sub-video segment, playing the video file according to the speed rate corresponding to the sub-video segment in the speed description file. According to the method, the speed multiplication rate can beautomatically calculated through the voice in the video, so that a user can watch the complete video at the most appropriate speed multiplication rate which is intelligently changed, the speed multiplication rate can be automatically and intelligently adjusted in the video speed multiplication playing process, manual adjustment of the user is not needed, and the user experience is good.
Owner:HUNAN HAPPLY SUNSHINE INTERACTIVE ENTERTAINMENT MEDIA CO LTD

Quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis

The invention discloses a quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis, which comprises the following steps of the selection and disinfection of explants, induction culture, multiplication culture, root induction culture and transplantation rooting. In the tissue culture propagation method for the narcissus tazetta var. chinensis, the complete technical process from the selection of the explants to the transplantation of the explants is accomplished, and bulbs subjected to the multiplication culture are rooted and induced within short time and are transplanted to a perlite substrate for rooting directly, so that test tube seedlings of the narcissus tazetta var. chinensis are not needed to be rooted in vitro; and the steps of induction rooting and transplantation are simplified, the production period is shortened, the cost is reduced, and the survival rate of the transplantation reaches over 92 percent. The method is high in pertinence, simple and easy and high in multiplication rate, produced seed bulbs are high in consistency and few in plant diseases and insect pests, the cost is saved, and the requirements for the industrial production of the narcissus tazetta var. chinensis seedlings can be met.
Owner:SHANGHAI BOTANICAL GARDEN

Windproof micro-pore pipeline spraying system for microbial sand stabilization and using method thereof

The invention provides a windproof micro-pore pipeline spraying system for microbial sand stabilization and a using method thereof. The integral structure of the windproof spraying system is composedof longitudinal and horizontal micro-pore pipelines, windproof covering films and curved nails. According to the windproof micro-pore pipeline spraying system and the using method thereof, a microbialbacteria solution and a the nutrient solution additive are sprayed into a soil body through the longitudinal and horizontal micro-pore pipelines, the reinforced soil body is prevented from being blown away by large air through the wind-proof covering films, so that the overall stability of the reinforced soil body is improved; and the windproof micro-pore pipeline spraying system has the advantages that the multiplication rate of microorganism in the soil body needing to be reinforced can be guaranteed, a relatively closed environment is maintained so that the reinforcement reaction can be carried out continuously, and a certain strength and the certain reinforcing effect are obtained. The windproof micro-pore pipeline spraying system for microbial sand stabilization and the using methodthereof can be used in microbial sand stabilization and wind prevention projects and can also be used in desert control projects.
Owner:HOHAI UNIV

Method for determining coaxial configuration micro-discharging threshold value

The invention discloses a method for determining a coaxial configuration micro-discharging threshold value. The method comprises the following steps of: determining an electronic movement locus and an electronic movement speed in a coaxial structure; converting probability of an emergence speed satisfying Maxwellian distribution into a joint probability density function of transit time; respectively carrying out maximum value and monotonicity processing to four classes of probability density functions to obtain a processed joint probability density function; taking an electron collision kinetic energy as an incidence electron energy of a secondary electron emission characteristic of a material to obtain a secondary electron multiplication function generated during electron collision under the transit time; constructing a steady-state equation which satisfies the electron number during micro discharging; judging whether a voltage generates micro discharging by solving an effective secondary electron multiplication rate in the steady-state equation; and gradually calculating an effective multiplication rate of a next voltage with a bisection method, wherein a corresponding voltage is a micro-discharging threshold value when the effective multiplication rate is 1. According to the method disclosed by the invention, an accurate micro-discharging threshold value can be obtained, and meanwhile, the threshold value can be quickly obtained.
Owner:XIAN INSTITUE OF SPACE RADIO TECH

Rooting culture method for pear test-tube plantlets and culture medium

The invention provides a rooting culture method for pear test-tube plantlets and a culture medium, and belongs to the technical field of plant cell engineering. The rooting culture method comprises the following steps: carrying out multiplication culture, strong seedling culture, induced rooting culture and large-amount rooting culture in sequence based on pear test-tube plantlets; the induced rooting culture step comprises the following sub-steps: sequentially inoculating tissue culture seedlings obtained after the strong seedling culture into a rooting culture medium A and a rooting culture medium B to be cultured to obtain rooting seedlings; and the large-amount rooting culture step comprises the following sub-steps: inoculating the rooting seedlings into a rooting culture medium C to be cultured, wherein the rooting culture medium A is a culture medium obtained by adding IAA, BA and NAA into a 1/4QL culture medium, the rooting culture medium B is a 1/4QL culture medium and the rooting culture medium C is a liquid culture medium obtained by adding IBA into a 1/3MS culture medium. The invention further provides the culture medium used in the method. By using the rooting culture method provided by the invention, the rooted test-tube plantlets with the relatively high multiplication rate, rooting rate, average rooting quantity and the relatively long average root length can be obtained.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Catalpa tissue culture rapid propagation method

ActiveCN107889744ANo vitrificationHigh effective proliferation ratePlant tissue cultureHorticulture methodsSucroseMultiplication rate
The invention provides a catalpa tissue culture rapid propagation method, and relates to the technical field of plant tissue culture. A catalpa tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The primary culture medium and the proliferation culture medium are Qj culture mediums: N6+6-BA 1.25 mg/L+IBA 0.07 mg/L+ agar 6.5 g/L+ sucrose 30g/L, and the rooting culture medium selects a Qg culture medium: 1/2MS+6-BA0.01mg/L+IBA 0.5mg/L+ agar 6.5g/L+ sucrose 15g/L. The method catalpa tissue culture rapid propagation method using the catalpa tissue culture medium includes the following steps: 1) obtaining sterile seedlings, to be more specific, selecting new shoots, treating the lengths, cleaning, disinfecting, sterilizing, cutting, inoculating into the Qj mediums, and cultivating to obtain sterile seedlings, 2) performing proliferation culture, to be more specific, treating the lengths of the sterile seedlings, transferringthe sterile seedlings to the Qj mediums, and cultivating to obtain subculture seedlings; 3) performing rooting culture, to be more specific, treating the subculture seedlings, inoculating the subculture seedlings into the Qg culture medium, and cultivating to obtain rooted seedlings; and 4) hardening seedlings and transplanting, to be more specific, transferring the rooted seedlings to a greenhouse for hardening the seedlings, transplanting the seedlings to an aperture disk, and transplanting after cultivation. The catalpa tissue culture rapid propagation method has the beneficial effects that effective multiplication rate of rooting culture is high, seedling rate is high, and factory production can be facilitated.
Owner:SHANDONG FOREST GERMPLASM RESOURCES CENT
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