Rooting culture method for pear test-tube plantlets and culture medium

A rooting medium and rooting culture technology, applied in the field of plant cell engineering, can solve the problems of not being able to adapt to production development, difficulty in rooting test-tube seedlings, and low reproduction coefficient, and achieve the effects of increased average root length, easy material selection, and stable genetic traits

Active Publication Date: 2014-12-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been some explorations on the in vitro culture of pears and their rootstocks, but pears are difficult to root fruit trees, wit

Method used

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  • Rooting culture method for pear test-tube plantlets and culture medium
  • Rooting culture method for pear test-tube plantlets and culture medium
  • Rooting culture method for pear test-tube plantlets and culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Rooting culture was carried out with pear test-tube plantlets. The pears tested were rootstocks Duli, Douli, and cultivars 'Sucui 1' and 'Sucui 2'. Adopt the following method to obtain the test-tube plantlets of rootstock Du pear, bean pear and varieties 'Su Cui No. 1' and 'Su Cui No. 2': adding a final concentration of 20-30g / L sucrose and a final concentration of 5-7g / L agar, take the tissue culture seedlings of each variety, cut 2cm new shoots and inoculate them in the above-mentioned medium, cultivate them at 22°C-25°C, with 16 hours of light and 8 hours of darkness every day, and the light intensity is 1500-2000lx. Subculture once every 15 days, and subculture twice to obtain rooted spare test-tube plantlets of each variety.

[0039] Carry out rooting culture respectively with rootstock Du pear, bean pear and varieties 'Sucui No. 1' and 'Sucui No. 2' for rooting standby test tube seedlings, and the specific methods are as follows:

[0040] 1) Proliferation cultur...

Embodiment 2

[0050] The rooting standby test-tube plantlets (obtained by method in embodiment 1) of rootstock Du pear, bean pear and varieties 'Su Cui No. 1' and 'Su Cui No. 2' are respectively carried out rooting culture, and specific methods are as follows:

[0051] 1) Proliferation culture: Cut out the new shoots of the spare test-tube plantlets for rooting, and transfer them to the proliferation medium for proliferation culture. Proliferation medium is a solid medium formed by adding BA, NAA, sucrose and agar to MS medium, in which the final concentration of BA is 0.5mg / L, the final concentration of NAA is 0.02mg / L, and the final concentration of sucrose is 20-30g / L, the final concentration of agar is 5-7g / L, pH=5.5-5.8. Culture conditions: at 22-25°C, 16 hours of light and 8 hours of darkness every day, light intensity of 1500-2000 lx, and culture time of 30 days.

[0052] 2) Cultivation of strong seedlings: Cut off the base of pear test-tube plantlets after multiplication and cultu...

Embodiment 3

[0059] The rooting standby test-tube plantlets (obtained by method in embodiment 1) of rootstock Du pear, bean pear and varieties 'Su Cui No. 1' and 'Su Cui No. 2' are respectively carried out rooting culture, and specific methods are as follows:

[0060] 1) Proliferation culture: Cut out the new shoots of the spare test-tube plantlets for rooting, and transfer them to the proliferation medium for proliferation culture. Proliferation medium is a solid medium formed by adding BA, NAA, sucrose and agar to MS medium, in which the final concentration of BA is 0.5mg / L, the final concentration of NAA is 0.02mg / L, and the final concentration of sucrose is 20-30g / L, the final concentration of agar is 5-7g / L, pH=5.5-5.8. Culture conditions: at 22-25°C, 16 hours of light and 8 hours of darkness every day, light intensity of 1500-2000 lx, and culture time of 30 days.

[0061] 2) Cultivation of strong seedlings: Cut off the base of pear test-tube plantlets after multiplication and cultu...

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Abstract

The invention provides a rooting culture method for pear test-tube plantlets and a culture medium, and belongs to the technical field of plant cell engineering. The rooting culture method comprises the following steps: carrying out multiplication culture, strong seedling culture, induced rooting culture and large-amount rooting culture in sequence based on pear test-tube plantlets; the induced rooting culture step comprises the following sub-steps: sequentially inoculating tissue culture seedlings obtained after the strong seedling culture into a rooting culture medium A and a rooting culture medium B to be cultured to obtain rooting seedlings; and the large-amount rooting culture step comprises the following sub-steps: inoculating the rooting seedlings into a rooting culture medium C to be cultured, wherein the rooting culture medium A is a culture medium obtained by adding IAA, BA and NAA into a 1/4QL culture medium, the rooting culture medium B is a 1/4QL culture medium and the rooting culture medium C is a liquid culture medium obtained by adding IBA into a 1/3MS culture medium. The invention further provides the culture medium used in the method. By using the rooting culture method provided by the invention, the rooted test-tube plantlets with the relatively high multiplication rate, rooting rate, average rooting quantity and the relatively long average root length can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and relates to a rooting culture method and culture medium for pear test-tube seedlings. Background technique [0002] Pear (Pyrus spp.) is an important woody economic fruit tree, ranking third in my country's fruit production after apples and citrus. In recent years, according to the main problems existing in the current varieties in various places and the needs of future development, the pear breeding work has achieved preliminary results, and many excellent varieties have been bred. How to expand the cultivation area of ​​new varieties on a large scale and promote the application of new varieties in the market has become an urgent problem to be solved at present. In recent decades, plant tissue culture technology, especially detoxification and micropropagation technology has been widely used in agriculture, and has become one of the most basic technologies in modern agricultura...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王宏蔺经常有宏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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