The invention provides a method for preparing a phalaenopsis plant tissue medium and relates to a plant tissue culture technology. The method for preparing the monstera deliciosa plant tissue medium adopts a tissue culture technology, the formula of the medium consists of a seed germination medium, a differential medium, a strong seedling medium and a rooting medium, and the four media are respectively prepared and applied. The formula of the seed germination medium comprises minimal medium Murashige and Skoog (MS), 6-benzyl aminopurine, 1-naphthaleneacetic acid, activated carbon, cane sugar and agar powder; the formula of the differential medium comprises minimal medium 1/3MS, the 6-benzyl aminopurine, the 1-naphthaleneacetic acid, the activated carbon, banana juice, the cane sugar and the agar powder; the formula of the strong seedling medium comprises the minimal medium MS, the 6-benzyl aminopurine, benzaole-3-butyric acid, hyponex No.1, hyponex No.2, the activated carbon, the cane sugar and the agar powder; the formula of the rooting medium comprises the minimal medium MS, the 1-naphthaleneacetic acid, the banana juice, the activated carbon, the can sugar and the agar powder; and the required medium products are respectively prepared and obtained according to the formulas, the phalaenopsis plant tissue culturing and high power breeding are realized.