1189results about How to "High rooting rate" patented technology

The invention provides a method for preparing a phalaenopsis plant tissue medium and relates to a plant tissue culture technology. The method for preparing the monstera deliciosa plant tissue medium adopts a tissue culture technology, the formula of the medium consists of a seed germination medium, a differential medium, a strong seedling medium and a rooting medium, and the four media are respectively prepared and applied. The formula of the seed germination medium comprises minimal medium Murashige and Skoog (MS), 6-benzyl aminopurine, 1-naphthaleneacetic acid, activated carbon, cane sugar and agar powder; the formula of the differential medium comprises minimal medium 1 / 3MS, the 6-benzyl aminopurine, the 1-naphthaleneacetic acid, the activated carbon, banana juice, the cane sugar and the agar powder; the formula of the strong seedling medium comprises the minimal medium MS, the 6-benzyl aminopurine, benzaole-3-butyric acid, hyponex No.1, hyponex No.2, the activated carbon, the cane sugar and the agar powder; the formula of the rooting medium comprises the minimal medium MS, the 1-naphthaleneacetic acid, the banana juice, the activated carbon, the can sugar and the agar powder; and the required medium products are respectively prepared and obtained according to the formulas, the phalaenopsis plant tissue culturing and high power breeding are realized.

The invention discloses a cuttage seedling culture method for red lagerstroemia speciosa and belongs to the field of seedling propagation culture. The method comprises the following steps that (1) treated yellow soil is adopted as culture substrates, and a sunshade net is erected above a seedling bed in May to June; (2) the cuttage is carried out in late June to early July, strong branches are adopted as cutting slips, grading is carried out, and the tray cuttage is carried out after the treatment by a rooting agent; (3) films are mulched after the cuttage, the temperature is controlled to be 25 to 28 DEG C, the humidity reaches 90 to 100 percent, and the pest disease prevention, treatment and management are carried out; and (4) the ventilation and the seedling exercising are gradually carried out after 20 days from the cuttage according to the rooting conditions. The method aims at the characteristics of the red lagerstroemia speciosa, greenhouse facilities and tray containers are utilized for summer cuttage seedling culture, the mass propagation and culture of the red lagerstroemia speciosa can be realized, qualified seedlings are provided for markets, meanwhile, the rooting rate and the survival rate of the red lagerstroemia speciosa are greatly improved. When the method provided by the invention is used for cuttage, the branches in the current year can grow to about 60 to 80cm, and the survival rate reaches higher than 95 percent.

The invention belongs to the technical field of plant seedling culture, and discloses a seedling growing method for oil expression peony seeds. The method comprises measures of collection and processing of the oil expression peony seeds, seed storage, seedbed preparation, sowing and seedling raising, management after transplantation, and the like. Compared with the prior art, the method has the advantages that the seed processing time is short, the seed processing method is simple, the emergence of seedlings is tidy, the emergence rate is high, the seedling survival rate is high, the seedling raising cost is low, no season influence of transplantation after seedling survival exists and the like.

The invention relates to a method for cuttage propagation of rhododendron lapponicum, which includes: making a mixture of peat and pearlite into cuttage substrate according to a volute ratio of from 1:3 to 1:4, cutting a stem of a current year growing semi-lignified branch of a rhododendron lapponicum plant which is under no pest and disease damage and 3-5 years old as a cutting, and retaining two to three complete leaves at the top of the cutting; dipping the base of the cutting into rooting reagent A or B prior to inserting into the substrate; and performing intermittent spray for subsequent water management, maintaining mass water content of soil to be 60-65%, and maintaining air humidity to be more than 80% during a period from cuttage to rooting of a cutting seedling. The rooting reagent A includes IBA (indole-3-butyric acid) 0.8-1.0% and thiophanate-methyl 2-4%. The rooting reagent B includes IBA 0.015-0.02% and thiophanate-methyl 1-2%. By the method, rooting rate can reach morethan 85%, rooting of the cutting only requires about 60 days, and production cycle is short.

The invention relates to a kind of plant rapid breeding method by utilizing plant re-tube non-sugar artificial heterotrophy procedure for supporting light auto tropic and realizing mixotrophic tissue culture, which comprises the follow procedures: built seedling bed in greenhouse canopy or field, built small hump hut and cover ventilating membranella; take out the plant segment that have leave or one-bud-one-leave as exsomatize material, use indolebutyric acid solution that every liter contain18-21 gram oxygen, the consistence is 110-220 ppm, treat 30-90 minutes, breed in the seedling bed; control the temperature, the air humidity, matrix humidity, sunshine, carbon dioxide consistence, disinfect, the entire valence inorganic nutrition liquor pass through the magnetic field that the magnetic flux is 0.4-0.6T or the alternating electric field that the density of field energy is 6-10KV / cm, the frequency is 15-26KHz,the nutrition liquor flux is 0.2 liter / s, after proceed magneticsation or electrolytic treatment activating, sprinkle the nutrition liquor.

The invention provides a method for rapidly culturing shoot tips in vitro, which is characterized in that in the first step, selecting stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step, cutting 0.1-0.5 mm shoot tips of the disinfected and cleaned strawberries stolon under the anatomical lens, inoculating to a culture medium for germination culture, cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step, cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring test tube plantlets to a substrate formed by mixing perlite and peat, watering and covering a plastic film for insulation and moisture retention, revealing the plastic film for gradually hardening, transferring the seedling to a plastic potted tray filled with pure peat, watering and covering the plastic film for insulation and moisture retention, and directly planting the seedling to a field. The invention has the advantages of high reproductive rate, rapid reproduction speed and high survival rate.

The invention relates to the technical field of fertilizer, in particular to water soluble fertilizer with a function of preventing and treating the citrus dieback and a preparing method of the water soluble fertilizer. The water soluble fertilizer is prepared by evenly mixing, by weight, 5-87% of phosphite, 10.0-50.0% of ureophil, 1.0-5.0% of EDTA zinc, 3.0-10.0% of EDTA magnesium, 1.0-10.0% of boron fertilizer, 0-0.5% of indole-3-butyric acid potassium and 0-0.5% of compound sodium nitrophenolate in a blender; through the synergistic function of all components, the synergistic effects are achieved for crop yield increase and preventing and treating the citrus dieback, the citrus dieback can be prevented, and nutrients of nitrogen, phosphorus, potassium, magnesium and boron are supplemented; the water soluble fertilizer is free of pollution and residue, low in cost, easy to manufacture and high in production effectiveness.

The invention relates to the field of crop planting, and particularly to a method for planting potatoes, which comprises the following steps: preparing land, fertilizing, and ditching; putting the potato seedlings into ditches, ridging, covering soil, covering parts on ridges, where the potato seedlings are planted, with colorless and transparent film, and covering two sides of the colorless and transparent film with black film; conducting field management, and harvesting potatoes. The method adopts the colorless and transparent film and the black film, which are in a matched manner, to plant the potatoes, the colorless and transparent film has good light transmittance and increases temperature quickly at the early stage, so the potatoes can root early; the black film has low visible light transmittance and can restrain weeds from growing, and the weeding rate can reach about 90 percent; during the middle and later periods of growth, the root systems enter below the black film, high temperature below the film, caused by strong sunlight, is prevented, and the premature senility of the root systems is avoided; the method can create a good environment for potato tuber growth in the period of tuberization, and has a ground temperature adjusting function that other film does not have; not only is the labor intensity of weeding reduced, but also the yield is increased by a large margin.

The invention discloses a cutting propagation method for Rhododendron simsii. The method includes the following steps; using a mixture of peat, perlite and vermiculite as a substrate; acquiring top branches with buds from canopy of plants in autumn or early spring, and cutting the top branches into cutting shoots having the length of 7-9 cm; removing a part of leaves, performing single-face healing treatment on the base of the cutting shoots, and then using 100 mg.L-1 IBA aqueous solution to soak the cutting shoots for 16 hours; instantly performing cuttage after treatment of the cutting shoots, wherein the spacing between rows is 8X8 cm, and the cuttage depth is 2 / 3 of the length of the cutting shoots; and using an automatic intermittent spraying system to supply water and increase the humidity during a rooting period, wherein the temperature is controlled at 15.0-20.0 DEG C, the relative humidity is 85.0-95.0%, and the Rhododendron simsii is sprayed with a bactericide every 14 days. The method can effectively solve the problem that the conventional Subsect. Maddenia Rhododendron simsii cutting propagation method is difficult to root, is low in rooting rate, and is few in root systems; the rooting rate can reach over 90%, and the root systems are developed; and the method is simple to operate, is low in cost, and can be used for industrialization seedling of the Rhododendron simsii.

Provided are a plant cultivation container, a plant cultivation method, and a method of producing a rooted cutting, in which a plant can be cultivated under a desired humidity condition, whereby a rooting rate can be enhanced and extension of a root can be promoted. In the plant cultivation container in which a plant cultivating medium (2) is provided in an inside of a container body (1) with a substantially hermetically sealed structure, a partition wall (3) that partitions the inside of the container body (1) into two upper portion and lower portion and supports the medium (2) is provided in the container body (1). The upper portion of the container body (1) defined by partitioning of the partition wall (3) constitutes a high-humidity chamber (4), and the lower portion of the container body (1) constitutes a low-humidity chamber (5). A bent hole (7) that allows the medium (2) and the low-humidity chamber (4) to communicate with each other is provided in the partition wall (3). A high-humidity state is achieved in the high-humidity chamber (4), and a low-humidity state is achieved in the low-humidity chamber (5). Low-humidity air is supplied from the vent hole (7) to the medium (2).

The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.

The invention provides a culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile, belonging to the technical field of plant cultivation. The culture medium comprises following substances according to weight percentages: 45%-80% of water, 3%-17% of an inorganic matter, 2%-15% of an organic matter, 0.1-7% of a plant growth regulator and 5%-30% of a synergic component. According to the culture medium for rapidly inducing the adventitious buds in the tissue culture of the dendrobium nobile, the requirements on nutritional ingredients caused by the dendrobium nobile in the tissue culture process and growth and development characteristics of the dendrobium nobile are utilized and the prepared culture medium can be used for obviously promoting callus of the dendrobium nobile to differentiate the adventitious buds; meanwhile, the rooting rate and the rooting quantity of the adventitious buds can be increased; the culture medium has the advantages of low production cost, convenience in utilization, safety in storage and transportation, high application and economic values, stability and high efficiency, rapidness in root developing and the like.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

The invention belongs to the technical field of the vegetative propagation and seedling cultivation technique for plants and particularly relates to a method for increasing the cutting rooting rate of pecans. The method for increasing the cutting rooting rate of pecans comprises the steps that a cutting shoot well cut from a seed tree is soaked in rooting powder processing liquid, ultrasonic treatment is conducted on the cutting shoot through an ultrasonic cell crusher at the same time, and then the cutting shoot is inserted into a matrix, wherein the power of the ultrasonic cell crusher ranges from 200 w to 300 w, ultrasonic treatment is conducted for 5 s to 7 s each time, the interval between every two sequential times of ultrasonic treatment is 5 s to 7 s, the total time of ultrasonic treatment is 2 min to 4 min, the temperature of the matrix ranges from 20 DEG C to 28 DEG C, and the water content of the matrix ranges from 20% to 40%. The method for increasing the cutting rooting rate of pecans has the advantages that the time is short, the rooting rate is high, and the number of cottage batches is large.

A cuttage raising method for olive seedlings on a greenhouse hotbed includes: preparing a cuttage bed, acquiring scions, treating cuttings, performing cuttage, selecting cuttage time and performing post-cuttage management; setting the cuttage bed in a solar greenhouse; selecting an annual young tree as a female tree to acquire the scions, and selecting highly mature shoots without disease and insect damage as the cuttings; cutting each cutting into a cutting 10-12cm long with 4-6 segments, reserving 2-4 leaves at the top end of the cutting, smoothing the position, 0.5-1cm away from a bud, of the top end by cutting, and cutting the lower end of the cutting into an inclined horse-ear-shaped end; soaking the root of each cut cutting in 1000mg / kg-2000mg / kg indolebutyric acid solution at a 5cmdepth for one night; on the next day, dipping each cutting with mixture of indolebutyric acid and talcum powder before cuttage; reserving row spacing of the cuttings to be 5-6cm and plant spacing to be 3-4cm; keeping interior temperature of the solar greenhouse to be 18-20 DEG C, humidity to be 90%-95%, cuttage bed soil temperature to be 23-25 DEG C and water content to be 60%-70%; moving the seedlings, which root for 45-55 days, off the bed. By the method, the rooting rate of cuttage seedlings reaches more than 90%, calluses emerge 35-40 days after cuttage, and the seedlings can root in 45-55 days.

The invention provides a method for tissue culture of paeonia lactiflora, which comprises the following steps of: sterilizing a paeonia lactiflora stem tip; and performing primary culture, secondary culture and rooting culture on the sterilized paeonia lactiflora stem tip, wherein the sterilization of the paeonia lactiflora stem tip is implemented by two steps to ensure that the stem tip is sterilized thoroughly, an explant is not damaged, and the survival rate of the stem tip is high. The method for tissue culture of the paeonia lactiflora is simple to operate, has a low cost and a high rooting rate, can realize transplantation out of a bottle, and is suitable for rapid propagation of most ornamental paeonia lactiflora varieties; and simultaneously, according to the culture method provided by the invention, the excellent characteristics of an original species can be kept, and a base is established for high-quality seed breeding and industrial production.

The invention relates to a seedling nutritional medium, which is prepared by mixing peat soil, perlite, vermiculite, sand and deep glutinous clay. When the seedling nutritional medium is used for culturing seedlings, the seedling nutritional medium can effectively promote rooting and growth of the seedlings and improve the rooting rate and the seedling rate of the seedlings so as to raise economic benefit.

The present invention discloses an air layering seedling cultivating method. The air layering seedling cultivating method comprises the following steps of: (1) branch processing; (2) branch wrapping; (3) management after wrapping; and (4) rooting and transplanting. Compared with the prior art, according to the air layering seedling cultivating method, land is not occupied before rooting, propagation time is long, the whole growing season can be used for propagation, materials are easy to get, the method is simple, rooting rate and transplanting survival rate are high, and the method is especially suitable for trees which are not easy to fruit and root after cutting, have no stem tillers or root tillers for division seedling cultivation, cannot be cultivated by a grafting method, and have high crowns.

The invention discloses a tissue culture and rapid propagation method for kadsura coccinea. The method includes the step A of selection and processing of explants, the step B of induction of explants, the step C of browning prevention processing of calluses, the step D of differentiation of calluses, the step E of subculture multiplication culture, the step F of strong seedling culture, the step G of rooting culture and the step H of seedling hardening and transplanting, wherein in the step A, young and tender leaves close to tips of kadsura coccinea stems are taken and disinfected to obtain sterile explants. By means of the method, the browning rate of explants of kadsura coccinea is controlled at 7% or below, the rooting rate reaches 95%, and the seedling formation rate reaches 97% or above.

The invention discloses a method for inducing blueberries to root by using liquid culture media and permeable matrix, comprising the following steps: (1) preparing rooting culturing media; (2) transferring the healthy and strong blueberry seedlings which are subcultured for 40d and are 3-4 cm high into the rooting culturing media, culturing the blueberry seedlings for 7-10d in the dark and for 12h / d for about 20d under intermitted illumination condition until the blueberry seedlings have 3-7 root systems of 0.5-2 cm long; and (3) transplanting the test-tube blueberry seedlings which are cultured for 30d to root, are 3-6 cm high and have the root length of 0.5-2 cm into the media in which the proportion of turfy soil to moss is 1:1, and managing the blueberry seedlings. The rooting culturing media is prepared according to the following substeps: 1) preparing 0.8 cm*1.2 cm filtering paper balls or defatted cotton balls or 0.2 cm*0.6 cm vermiculites which function as the matrix of the rooting culturing media; 2) preparing rooting culturing solution which has the WPM of 1 / 2, the IBA of 1.0-5.0 mg.L-1, the edible white sugar of 2 percent and the pH value of 5.0-5.2; and 3) adding 6-10 filtering paper balls or defatted cotton balls or 20-30 vermiculites which are soaked by the rooting culturing solution and adding 3-5 ml rooting culturing solution to each culturing bottle and capping the culturing to sterilize according to the routine method. The invention has short rooting period and low production cost, can ensure that 100 percent of the blueberry seedlings can root and over 95 percent of the transplanted blueberry seedlings can survive and realize the rapid industrialized propagation of the blueberry seedlings on a large scale.

The invention discloses a method for cultivating a subcultured bud of an upright crown tissue culture seedling of fir wood. The method comprises the following steps: an excellent clonal annual burgeon of the fir wood is used as an explant, a breeding bud is obtained through disinfection, sterilization and tissue culture, the breeding bud is innoculated in a proliferation culture medium comprising 1 / 2MS, 1.0mg / L of 6-BA and 0.5mg / L of IBA for inducing the cluster buds to sprout, the subcultured bud which is germinated at the base of the breeding bud and is healthy in growth is selected and is cut in a common rooting culture medium, seedlings are exercised and transplanted, and when the height of the seedlings of the fir wood is 15-30 cm, the seedlings are taken out of a garden for forestation. When the method is used, the proliferation rate of the subcultured bud of the fir wood is as high as 4-6, the breeding speed is high, the seedling culturing number is large, the tissue culture subcultured bud with high genetic stability, 100% of the upright crown rate of the tissue culture seedling and more than 96% of the rooting rate can be in batch production in a short period, and the requirements of the construction and the development of a fir wood reserving base of China for good seedlings can be met, so that the method has better economic benefits, social benefits and ecological benefits.

The invention provides a fir tissue culture rooting method. Small fir multiplication subculture seedlings are cut and transplanted to a fir rooting culture medium for culturing and rooting tissue, and the fir rooting culture medium comprises an improved MS culture medium, 0.4-0.8mg / L of ABT1#, 0.1-0.5mg / L of IBA and 2-6mL / L of root sun diluent. After the fir rooting culture medium is adopted, the rooting time of firs is shortened to 16 to 22 days; after 22 days of culture, the highest rooting rate achieves 96 percent, and nursery stocks grow rapidly so as to decrease the culture cost of the seedlings and realize the industrialized production of the firs.

The invention discloses a tissue-culture rapid propagation method for pyrus betulaefolia bunge. The tissue-culture rapid propagation method for the pyrus betulaefolia bunge comprises disinfecting on explants, inducing buds, propagating test tube seedlings, inducing calluses, inducing rooting and transplanting exercising seedlings. During the process of inducing rooting, the calluses are firstly induced and then the rooting is induced; obtained rooting test tube seedlings are performed seedling exercising in a triangular flask, then transferred to perlite to domesticate the exercising seedling and finally transplanted. Compared with the traditional method, the tissue-culture rapid propagation method for the pyrus betulaefolia bunge has the advantages of being high in rooting rate and survival rate of transplanting, high in propagation rate, low in cost, convenient to popularize, good in genetic stability and suitable for the pyrus betulaefolia bunge.

The invention discloses a tissue culture medium of sealwort roots and stems and an in-vitro regeneration method of the tissue culture medium. The tissue culture medium comprises an inducing culture medium, a proliferation culture medium and a rooting culture medium. The method using the tissue culture medium of the sealwort roots and stems for carrying out in-vitro regeneration on the sealwort roots and stems comprises the three steps of induction of sprouts, proliferation culture of the sprouts and rooting culture. The tissue culture medium and the propagation method have the beneficial effects that a system of in-vitro regeneration of the sealwort roots and stems is established by utilizing a tissue culture technology, and the inducing culture medium, the proliferation culture medium and the rooting culture medium in the method are selected and optimized; and the tissue culture medium has the advantages of high sprout induction rate, high proliferation coefficient, high rooting rate, strong sprouts and seedlings, normal leaf shape and color and the like, and can meet the need of the market for the sealwort roots and stems.

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

The invention provides a camellia chrysantha tissue culture propagation method. The camellia chrysantha tissue culture propagation method comprises the steps of obtaining aseptic seedlings, propagating the aseptic seedlings, culturing strong multiple shoots, expanding propagation and carrying out rooting culture, wherein camellia chrysantha embryos are selected as explants and then inoculated to a primary culture medium to obtain the aseptic seedlings of the camellia chrysantha; next, the aseptic seedlings are inoculated to a subculture multiplication culture medium and a strong shoot culture medium; the seedling propagation and strengthening processes are repeated and circulated until a large quantity of strong aseptic seedlings are obtained; finally, the aseptic seedlings are transplanted to a rooting culture medium for rooting culture. The camellia chrysantha tissue culture propagation method has the advantages that the vitrification problem of the aseptic seedlings in the camellia chrysantha tissue culture process is overcome, multiple subculture of materials can be realized, large-scale propagation is realized, the seedling growing period of the camellia chrysantha is shortened, the seedling growing materials are saved, the rooting rate is 95%-98%, and the transplanting survival rate is more than 90%; as a result, the camellia chrysantha tissue culture propagation method has excellent economic benefit, social benefit and ecologic benefit.

The invention relates to a cuttage and rapid propagation method for camellias. The implementation of the method includes the following concrete steps that (1), a cuttage bed with perlite as a matrix is built; (2), a humidity control system and an automatic spray system are built, and according to the humidity changing condition on the surface of the matrix, spray equipment is controlled to provide and adjust the rooting and growing environment for cuttings; (3), robust annual or one-year-old branches above half lignifications and free from plant diseases and insect pests are selected as cuttings, and shearing and cuttage are carried out on the cuttings in time; (4), before cuttage is carried out, the cuttings are treated with a rooting agent to promote rooting; (5), after cuttage is carried out, the spray facility is started, humidity parameters are set, the spray time is adjusted after 40 days, seedlings can be lifted and put in flowerpots 60 days after cottage, and the seedlings are transplanted 15 days after seedling hardening. According to the cuttage and rapid propagation method for the camellias, the nursery stock propagation cycle is short, the investment cost is low, and the cuttage and rapid propagation method can be widely used for large-scale and industrial garden and park nursery stock development by individuals and enterprises.

The invention discloses a cuttage propagation method for Zelkova, which belongs to the field of plant cultivation and includes: using a Zelkova shoot as cutting wood; quickly dipping the cutting wood into high concentration rooting agent; and subjecting the dipped cutting wood to a substrate in a high humidity environment. By means of strict planting management and a scientific transplanting colonization scheme, rooting rate of Zelkova in cuttage propagation is increased greatly, and the method has high significance and promotion effect on quick propagation of Zelkova.

The invention discloses a tissue culture method of gynura divaricata. The method comprises the steps of: inductive culture: inoculating on MS start medium without any hormone to induce generation of axillary bud; multiplication culture: inoculating on the medium MS+6BA, 0.2 mg / L+sucrose 20 g / L+ of agar 6 g / L for multiplication; and rooting culture: rooting on MS+IBA 0.1 mg / L+sucrose 20 g / L+agar 6 g / L, or MS+IBA 0.3 mg / L+IAA 0.3 mg / L+sucrose 20 g / L+agar 6 g / L, wherein the culture temperature is 25+ / -1 DEG C, the illumination intensity is 20,001x, and the illumination time is 16 h / d. The method disclosed by the invention can quickly and efficiently produce healthy and uniform high-quality gynura divaricata seedlings with pure characteristics at large scale.