11results about How to "High rooting rate" patented technology

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The invention provides a method for promoting aeroponic rooting of pinus khasys, and relates to the technical field of cutting propagation of pinus khasys. The method for aeroponically culturing and rooting the pinus khasys comprises the following steps: soaking scions of the pinus khasys in a citric acid solution in an ice-water bath, disinfecting, treating with a rooting agent, and aeroponically culturing by adopting a nutritional agent added with calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indolebutyric acid, sodium phytate and lauryl alcohol polyether-4. According to the method, the defects in the prior art are overcome, rapid rooting of the pinus kesiya branches is effectively promoted, growth of the root system is guaranteed, a rapid and convenient method is provided for stable seedling raising of the pinus kesiya, and artificial breeding of the pinus kesiya is guaranteed.

The application provides a sugarcane tissue culture method, and belongs to the technical field of sugarcane tissue culture, and the tissue culture method comprises the following steps: (1) taking sugarcane tender stems as explants, alcohol disinfection, and obtaining pretreated explants; (2) placing the pretreated explants in an induction culture medium, inducing culture, and obtaining callus; (3) placing the callus in a proliferation culture medium, proliferating culture, and obtaining adventitious buds; (4) placing the adventitious buds in a rooting culture medium, rooting culture, and obtaining tissue culture seedlings; (5) placing the tissue culture seedlings in a seedling raising substrate, seedling raising, and obtaining seedlings; the seedling raising substrate in step (5) comprises the following raw material components in mass parts: 10-20 parts of sugarcane residues, 40-60 parts of peat soil, 10-15 parts of sheep manure, and 8-10 parts of rice husk charcoal. The tissue culture method provided by the application can improve the rooting rate and transplanting survival rate of sugarcane tissue culture seedlings, and the growth traits of the tissue culture seedlings can also be significantly improved.

This invention belongs to the field of hop propagation technology, specifically relating to a method for propagating hops by rock wool cuttings in plug trays. The method for propagating hops by rock wool cuttings in plug trays includes the following steps: (1) Preparing cuttings: Selecting hop mother plants and cutting semi-lignified branches as cuttings; (2) Treating cuttings: After cutting the cuttings, soaking the base in a plant growth regulator; (3) Cutting operation: Dividing the rock wool into small pieces, placing them in plug trays, completely soaking the rock wool in a probiotic solution, and inserting the cuttings into the rock wool; (4) Post-cutting management: Placing the plug trays after cutting in a greenhouse or polytunnel, cultivating for 45-50 days after cutting, and then transplanting. The propagation method of this invention can improve the rooting rate of hops, while the root system of the cuttings is more developed, the plants grow better, and the survival rate after transplanting is higher.

The invention provides application of agrobacterium rhizogenes in promoting rooting of fructus forsythiae, and belongs to the technical field of rapid vegetative propagation of plants. The method for promoting rooting of fructus forsythiae comprises the following steps: (1) inoculating agrobacterium rhizogenes into a culture medium for activation culture; 2) performing amplification culture on the activated and cultured bacterial liquid, collecting the bacterial liquid, and re-suspending the bacterial liquid to obtain a re-suspended bacterial liquid; according to the method, complex equipment is not needed, operation is easy and convenient, the cost is low, the rooting rate of fructus forsythiae is remarkably increased, the rooting time is shortened, and the method is suitable for large-scale seedling production.

The present application relates to the technical field of plant tissue culture, and provides a tissue culture method for promoting germination of Zingiber zerumbet Smith, wherein Zingiber zerumbet Smith tubers are used as explants, and after being subjected to disinfection treatment, the explants are inoculated into an induction medium, a proliferation medium and a rooting medium in sequence, and then are subjected to acclimatization, seedling raising and transplanting, so that finished Zingiber zerumbet Smith seedlings are obtained. The germination rate, the proliferation coefficient and the rooting rate of the Zingiber zerumbet Smith tubers can be significantly improved by using the culture media which are optimized and adjusted in the technical scheme of the present application. In addition, the reasonable application of foliar fertilizer and fungicide after transplantation in the present application can further improve the growth vigor and the disease resistance of Zingiber zerumbet Smith seedlings, and ensure the survival rate of the seedlings, thereby providing reliable technical support for rapid propagation and large-scale planting of Zingiber zerumbet Smith.

The present application relates to the field of Ranunculus Beijingensis culture and propagation, and discloses a Beijing Ranunculus culture and propagation method based on environment simulation, comprising the following steps: collecting the environment parameters of the original habitat of Beijing Ranunculus, and building a precisely matched artificial environment simulation culture system; then obtaining sterile explants through stem segment screening and gradient sterile treatment, and completing adventitious bud induction, proliferation and streamlet-imitating liquid rooting and seedling strengthening through optimized medium formula; and finally realizing efficient propagation through simulated habitat acclimatization, transplanting and planting. The present application combines real-time simulation of original habitat parameters with tissue culture technology, solves the problems of explant pollution and browning, low proliferation efficiency and poor transplanting adaptability in traditional methods, and significantly improves the rooting rate and transplanting survival rate.

The application discloses a culture method for improving rooting efficiency of Phoebe sheareri tissue culture seedlings, and the rooting efficiency of the Phoebe sheareri tissue culture seedlings is improved through multiple steps of seed disinfection, germination culture, external hormone treatment, active carbon adsorption of secondary metabolites and culture medium optimization, so that the tissue culture efficiency of the Phoebe sheareri is systematically optimized and improved, the survival rate of the Phoebe sheareri tissue culture is improved, and the rooting rate of the Phoebe sheareri is improved from 6.57% to 100%. In addition, the addition of active substances significantly promotes the bolting growth of the Phoebe sheareri, and the bolting rate is increased by about 20% compared with the culture medium without the addition, thereby providing strong support for the subsequent growth of the Phoebe sheareri, especially in the rapid growth of the Phoebe sheareri plant, and providing technical support for commercial application and forestry resource development.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.

The invention discloses application of PSK to improvement of asexual propagation efficiency of lily scales, belongs to the technical field of plant asexual propagation, and particularly aims to solve the problems that existing lily scale cutting propagation is low in efficiency, slow in budding and rooting and the like. The PSK with the effective concentration (preferably 10 nM <-1 > mu M) is prepared into a treatment solution; during cuttage, the water is applied as first-time irrigation liquid, and the physiological response of the scales can be systematically regulated and controlled. Experiments show that the application can significantly improve the budding rate of longya lilium scales, promote the expansion of new bulblets, and synchronously improve the rooting rate. The invention provides a novel efficient asexual propagation method for lily, which is simple and convenient to operate, remarkable in effect and safe, and is suitable for large-scale production of lily seedlings.