29results about How to "High rooting rate" patented technology

The invention relates to the field of plant tissue culture, and discloses a method for culturing tissue culture seedlings of small angel viridae, which comprises the following steps: S1, explant selection and primary culture; s2, carrying out subculture multiplication culture; s3, rooting culture; s4, domesticating and transplanting; in the primary culture stage, MS is used as a basic culture medium, a proper amount of 6-BA, NAA, sucrose and agar are added, and induction of cluster buds is promoted; in the subculture multiplication culture stage, an optimal multiplication culture medium is screened through an orthogonal test, so that the multiplication coefficient is improved; in the rooting stage, key factors such as inorganic salt concentration, NAA and banana puree in MS are optimized, and the number and quality of root systems are improved; in the domestication transplanting stage, the optimal proportion of river sand, coco coir and peat soil is researched, and the transplanting survival rate and the tissue culture seedling quality are effectively improved. By means of the method, efficient propagation of the small angel vitis vinifera and large-scale production of high-quality seedlings are achieved, and important technical support is provided for industrial popularization of the plant.

The invention relates to motherwort cultivation. The leonurus japonicus rooting induction method is characterized by comprising rooting induction: when adventitious buds grow to 1-3 cm, the adventitious buds are cut off from explants and transferred into a rooting culture medium to be cultured, the rooting culture medium takes an MS culture medium as a basic culture medium, and IBA with the concentration of 0.1 mg.L <-1 > is added into the rooting culture medium. The components of the culture medium are selected, and the rooting rate of the selected rooting culture medium is high.

The invention discloses a tissue culture and rapid propagation method for a fig male tree germplasm material, and belongs to the technical field of plant tissue culture and rapid propagation. The specific method comprises the following steps: (1) obtaining and disinfecting an explant; (2) primary culture of buds; (3) subculture multiplication of buds; (3) rooting culture; and (4) hardening seedlings and transplanting. According to the method, the optimal culture medium for tissue culture and rapid propagation of the fig male tree is obtained by combining different hormones and adjusting different hormone concentration ratios, and the differentiation and proliferation of buds of the fig male tree and the growth and rooting rate of seedlings are remarkably improved. In the explant treatment, tebuconazole carbendazim, 75% alcohol and sodium hypochlorite are adopted for the first time, so that the use of a highly toxic reagent mercury bichloride is avoided; cefotaxime sodium is added into a primary culture medium, so that the pollution rate of explants is reduced; the method is simple to operate, time-saving, labor-saving, safe and environment-friendly, and improves the inoculation quality and efficiency. The method is not limited by seasons, enlarges the excellent male tree population of the figs in a short time, and lays a foundation for cultivating new germplasm of the figs.

The invention discloses an optimization method of a pea tissue culture regeneration system. The optimization method comprises the following steps: step 1, preparing pea aseptic seedlings; step 2, preparing an explant; step 3, inducing cluster buds; 4, carrying out bud elongation culture; step 5, rooting culture; and 6, domesticating and transplanting regenerated seedlings. By systematically optimizing the tissue culture conditions of the peas, an efficient regeneration system is established and is suitable for multiple pea varieties, and the problem of low regeneration efficiency in the prior art is solved. By means of the optimization method, researchers can obtain high-efficiency regenerated pea plants in a short time, the success rate and rooting efficiency of tissue culture regeneration are improved, meanwhile, the survival rate of regenerated seedlings is increased, and an important technical guarantee is provided for molecular breeding and gene modification of peas and other leguminous crops.

The invention relates to the technical field of plant cutting propagation, in particular to a method for cutting propagation of twigs of dalbergia humilis. The method comprises the following steps that 1, cutting environmental factors are set in a closed greenhouse, the light transmittance is 70%-75%, and spraying is conducted regularly so that the humidity can be kept at 85%-90%; 2, cutting slips are sheared, and cutting branches are selected as semi-lignified middle parts; 3, selecting a cutting medium, wherein the cutting medium is loess; and 4, hormone treatment: soaking the cutting slips in a 250 mg / L IBA solution for 30 minutes, then performing cuttage, and inserting half branches into the substrate. According to the technical scheme, by controlling the cutting time, the cutting environment, the cutting treatment, the cutting medium and other factors, the cutting rooting rate and the survival rate of the twigs of the dalbergia huang are remarkably increased, and the rooting rate is increased by 20%-50% or above.

The invention belongs to the field of plant tissue culture, and particularly relates to a culture medium composition and method for inducing plant regeneration through peony petioles, and the culture medium composition is characterized in that a callus induction tissue culture medium and an adventitious bud differentiation culture medium are MS culture mediums containing 0.5-1.5 mg / L TDZ and 0.05-0.25 mg / L NAA; the rooting induction culture medium is a 1 / 2 MS culture medium containing 2.0 to 4.0 mg / L of IBA, 0.3 to 0.7 mg / L of NAA and 0.4 to 0.6 g / L of TA. Petioles of peony sterile test-tube plantlets are used as explants; inoculating the explant into the callus induction culture medium for dedifferentiation culture to obtain a callus; inoculating the callus into the adventitious bud differentiation culture medium to induce bud differentiation to obtain adventitious buds; dividing the adventitious buds, and inoculating the adventitious buds into the rooting induction culture medium to induce rooting, so as to obtain regenerated seedlings. The culture medium is combined with a tissue culture method, petioles of peony seedlings are adopted for tissue culture, plant seedlings are obtained, the tissue culture seedling obtaining efficiency is high, the adventitious bud and adventitious root induction effect is good, and the transplanting survival rate is high.

The invention discloses a kudzu vine root seedling raising method. The disclosed method comprises the following steps: sequentially soaking kudzu vine root cuttings in a carbendazim solution and a growth solution; the growth solution is a mixed solution of growth hormone and an aqueous extract of fragrant eupatorium herb; the radix puerariae cuttage branches are lignified kudzu vines with bud nodes; performing cuttage on the soaked cuttings in a rooting medium for culture; the temperature of the culture environment is 26-30 DEG C, and the humidity is 76%-82%; when the cuttings are grown and buds grow to 5cm, applying a nutrient solution to the roots; and continuing to culture until the cutting wood roots and the root hair grows to 10cm to obtain the kudzu vine root seedlings. According to the method, the culture mode, the culture medium and the culture device are improved, so that the root primordium forming number of the cuttings is effectively increased, the rooting rate is increased, and the seedling raising period is shortened.

The invention discloses a method for inducing apocarya stems to generate transgenic hairy roots, and belongs to the field of forest tree genetic engineering. According to the method, the semi-lignified or lignified stems of the apocarya are infected by utilizing the agrobacterium rhizogenes to obtain stable transgenic hairy roots, rooting can be induced within 1-2 months, the rooting rate is as high as 94.43%, and the transgenic positive rate can reach 98.38%. The invention provides an economical and efficient non-tissue culture dependent transgenic method, it is clear that transgenic roots can be used for gene function verification, the method breaks through the technical obstacle that stable genetic transformation of the carya illinoensis is difficult, and technical support is provided for research of functional genes.

The invention relates to the technical field of agriculture, in particular to a pelleting formula of isatis tinctoria seeds capable of resisting root diseases and promoting rooting. The formula comprises the following components: isatis tinctoria seeds, an antifungal agent, a rooting accelerant, a humectant, a nutrition additive and an adhesive. The pelleting formula is prepared by the following steps: firstly, uniformly mixing isatis tinctoria seeds with an antifungal agent; adding a rooting accelerant, and continuously stirring; adding the humectant, and fully mixing; adding a nutrition additive, and uniformly mixing with the seeds; adding an adhesive, and continuously stirring; feeding the mixture into a granulator for granulation to form seed pellets with the diameter of 3-5mm; and drying the prepared seed pellets, and packaging after quality inspection. According to the isatis tinctoria seed pelleting formula capable of resisting root diseases and promoting rooting provided by the invention, the disease resistance and rooting rate of the seeds are remarkably improved by optimizing formula components and a preparation process.

The invention belongs to the technical field of agricultural planting, and discloses a picrorhiza scrophulariaceae seed stem cultivation and cutting seedling raising composition and method, and the composition is composed of naphthylacetic acid, indoleacetic acid, hymexazol, carbendazim wettable powder and a water medium; in the cultivation process of the picrorhiza scrophulariiflora seed stems, the thick and strong degree of the picrorhiza scrophulariiflora seed stems is reduced and the branch number and the branch length of overground stems are increased through fertilizer and water regulation, so that more propagation materials are obtained; then cutting the seed stem into small sections of 3-5cm so as to increase the propagation coefficient to a greater extent; meanwhile, a formula and method for seed soaking (graded seed soaking) and seedling stage water and fertilizer management are established, and the rooting rate and the seedling rate are increased; compared with traditional division propagation, the propagation coefficient of the picrorhiza seedling cultivated by the method is increased from 1: 5 to 1: 30-50 and is increased by more than 6 times.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The invention provides a culture method of begonia tissue culture seedlings, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) sterilizing begonia explants, inoculating the begonia explants into a detoxification culture medium, and performing detoxification culture to obtain cluster buds; (2) inoculating the cluster buds into an induction culture medium, and performing induction culture to obtain new buds; (3) inoculating the new buds into a proliferation culture medium, and carrying out proliferation culture to obtain clustered adventitious buds; and (4) inoculating the clustered adventitious buds into a rooting culture medium, and performing rooting culture and seedling hardening to obtain begonia tissue culture seedlings. According to the method, the begonia tissue culture seedlings are cultured by using the specific induction culture medium, the proliferation culture medium and the rooting culture medium, so that the proliferation coefficient, the rooting average number, the rooting rate and the adventitious bud number of the begonia tissue culture seedlings are obviously improved. Meanwhile, the tissue culture seedlings are cleaned after seedling hardening, and the relative transplanting survival rate of the tissue culture seedlings is increased. The method can be used for industrialized seedling production, and solves the problems of low reproduction rate and non-uniform growth uniformity in the existing cultivation method.

The invention provides a method for promoting aeroponic rooting of pinus khasys, and relates to the technical field of cutting propagation of pinus khasys. The method for aeroponically culturing and rooting the pinus khasys comprises the following steps: soaking scions of the pinus khasys in a citric acid solution in an ice-water bath, disinfecting, treating with a rooting agent, and aeroponically culturing by adopting a nutritional agent added with calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indolebutyric acid, sodium phytate and lauryl alcohol polyether-4. According to the method, the defects in the prior art are overcome, rapid rooting of the pinus kesiya branches is effectively promoted, growth of the root system is guaranteed, a rapid and convenient method is provided for stable seedling raising of the pinus kesiya, and artificial breeding of the pinus kesiya is guaranteed.

The application provides a sugarcane tissue culture method, and belongs to the technical field of sugarcane tissue culture, and the tissue culture method comprises the following steps: (1) taking sugarcane tender stems as explants, alcohol disinfection, and obtaining pretreated explants; (2) placing the pretreated explants in an induction culture medium, inducing culture, and obtaining callus; (3) placing the callus in a proliferation culture medium, proliferating culture, and obtaining adventitious buds; (4) placing the adventitious buds in a rooting culture medium, rooting culture, and obtaining tissue culture seedlings; (5) placing the tissue culture seedlings in a seedling raising substrate, seedling raising, and obtaining seedlings; the seedling raising substrate in step (5) comprises the following raw material components in mass parts: 10-20 parts of sugarcane residues, 40-60 parts of peat soil, 10-15 parts of sheep manure, and 8-10 parts of rice husk charcoal. The tissue culture method provided by the application can improve the rooting rate and transplanting survival rate of sugarcane tissue culture seedlings, and the growth traits of the tissue culture seedlings can also be significantly improved.

This invention belongs to the field of hop propagation technology, specifically relating to a method for propagating hops by rock wool cuttings in plug trays. The method for propagating hops by rock wool cuttings in plug trays includes the following steps: (1) Preparing cuttings: Selecting hop mother plants and cutting semi-lignified branches as cuttings; (2) Treating cuttings: After cutting the cuttings, soaking the base in a plant growth regulator; (3) Cutting operation: Dividing the rock wool into small pieces, placing them in plug trays, completely soaking the rock wool in a probiotic solution, and inserting the cuttings into the rock wool; (4) Post-cutting management: Placing the plug trays after cutting in a greenhouse or polytunnel, cultivating for 45-50 days after cutting, and then transplanting. The propagation method of this invention can improve the rooting rate of hops, while the root system of the cuttings is more developed, the plants grow better, and the survival rate after transplanting is higher.

The invention discloses a Pandanus tectorius cutting propagation method, and relates to the technical field of plant tissue propagation, the Pandanus tectorius cutting propagation method comprises the following steps: mixing river sand and red soil according to a volume ratio of (3-6): 1 to prepare a cutting medium, and disinfecting; a robust mother plant of pandanus tectorius is selected, semi-lignified and well-growing branches are cut to serve as cutting slips of the pandanus tectorius, and the base diameter of the cutting slips of the pandanus tectorius is 2-4 cm; the pandanus tectorius cutting slips are soaked with a naphthylacetic acid nano rooting agent; the pandanus tectorius cutting slips soaked with the rooting agent are subjected to cuttage and disinfection. The matrix formed by mixing the river sand and the red soil according to the proportion is good in drainage performance and air permeability, and the moderate humidity can be kept. The epidermis of the pandanus tectorius cutting slips is perforated, so that organic nutrition conveying from top to bottom can be slowed down, calluses are formed at the perforated positions, and the rooting rate is increased. According to the scheme, the rotting condition of the pandanus tectorius cutting slips can be effectively reduced, and the rooting rate of the pandanus tectorius cutting slips is increased.

The invention discloses a magnolia wufengensis tissue culture rapid propagation method which comprises the following steps: taking axillary buds wrapped in flower buds and epiphytic stem segments thereof as explants, and carrying out induction culture, multiplication culture and rooting culture on the sterile explants to obtain tissue culture plants. According to the method, the axillary buds with stems in the flower buds are selected as the explants, and the steps of disinfection, induction culture, multiplication culture and rooting culture are combined, so that tissue culture and rapid propagation of the magnolia wufengensis are successfully realized. According to the method, the propagation efficiency is remarkably improved, the pollution rate is reduced, the genetic stability of the tissue culture seedlings is ensured, and an effective technical means is provided for commercial production of the magnolia wufengensis and popularization of the ornamental value of the magnolia wufengensis.

The invention provides application of agrobacterium rhizogenes in promoting rooting of fructus forsythiae, and belongs to the technical field of rapid vegetative propagation of plants. The method for promoting rooting of fructus forsythiae comprises the following steps: (1) inoculating agrobacterium rhizogenes into a culture medium for activation culture; 2) performing amplification culture on the activated and cultured bacterial liquid, collecting the bacterial liquid, and re-suspending the bacterial liquid to obtain a re-suspended bacterial liquid; according to the method, complex equipment is not needed, operation is easy and convenient, the cost is low, the rooting rate of fructus forsythiae is remarkably increased, the rooting time is shortened, and the method is suitable for large-scale seedling production.

The present application relates to the technical field of plant tissue culture, and provides a tissue culture method for promoting germination of Zingiber zerumbet Smith, wherein Zingiber zerumbet Smith tubers are used as explants, and after being subjected to disinfection treatment, the explants are inoculated into an induction medium, a proliferation medium and a rooting medium in sequence, and then are subjected to acclimatization, seedling raising and transplanting, so that finished Zingiber zerumbet Smith seedlings are obtained. The germination rate, the proliferation coefficient and the rooting rate of the Zingiber zerumbet Smith tubers can be significantly improved by using the culture media which are optimized and adjusted in the technical scheme of the present application. In addition, the reasonable application of foliar fertilizer and fungicide after transplantation in the present application can further improve the growth vigor and the disease resistance of Zingiber zerumbet Smith seedlings, and ensure the survival rate of the seedlings, thereby providing reliable technical support for rapid propagation and large-scale planting of Zingiber zerumbet Smith.

The present application relates to the field of Ranunculus Beijingensis culture and propagation, and discloses a Beijing Ranunculus culture and propagation method based on environment simulation, comprising the following steps: collecting the environment parameters of the original habitat of Beijing Ranunculus, and building a precisely matched artificial environment simulation culture system; then obtaining sterile explants through stem segment screening and gradient sterile treatment, and completing adventitious bud induction, proliferation and streamlet-imitating liquid rooting and seedling strengthening through optimized medium formula; and finally realizing efficient propagation through simulated habitat acclimatization, transplanting and planting. The present application combines real-time simulation of original habitat parameters with tissue culture technology, solves the problems of explant pollution and browning, low proliferation efficiency and poor transplanting adaptability in traditional methods, and significantly improves the rooting rate and transplanting survival rate.

The invention discloses an ornamental shallot planting method, and belongs to the technical field of plant rapid propagation, and the method comprises the following steps: (1) explant disinfection; (2) callus induction; (3) differentiation culture; (4) multiplication culture; (5) rooting. According to the ornamental shallot planting method, the reproduction rate of ornamental shallots in each link from explant treatment to callus induction and adventitious bud induction to adventitious bud proliferation and finally to rooting is very high, the characters are stable, the excellent characters of female parents are kept to the maximum extent, and the ornamental shallots have good economic benefits. The invention establishes a good rapid propagation mode of the ornamental green onion, lays a foundation for research work such as new variety cultivation and genetic improvement on the variety by using a plant biotechnology in future, and also provides technical guidance and reference for establishment of a tissue culture rapid propagation technology of other plant varieties.

According to the hydroponic nutrient solution suitable for indoor soilless cutting propagation of the tea trees and the method for performing cutting rooting, germination and mechanical picking by using the hydroponic nutrient solution provided by the invention, by using the nutrient solution and the conditioning agent, rapid rooting and periodic germination of the hydroponic tea seedlings can be realized, and new shoots of the tea seedlings are suitable for mechanical picking; therefore, the problems of poor rooting rate of tea tree cutting cuttage, irregular germination and mechanical shoot picking are effectively solved.

The invention discloses a spring orchid CgARF4 gene and its application. The nucleotide sequence of the CgARF4 gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. The invention clones the gene sequence of CgARF4 from the spring orchid cultivar "Songmei", performs expression analysis in the spring orchid, then constructs it into an overexpression vector and introduces it into a target plant to verify its function. It is found that the Arabidopsis thaliana overexpressing the CgARF4 gene has an early flowering period, but the fruit is partially sterile, and the fruit setting rate is reduced compared with the wild-type plant. The rooting rate of the detached leaves on the MS medium containing IAA is significantly improved, the root length is increased, the content of endogenous hormones IAA, GA, and ABA in the leaves is reduced, and the content of MeJA and BR is increased. It can be seen that the gene will have a wide range of uses in the cultivation and genetic improvement of orchids and other horticultural plants.

The application discloses a culture method for improving rooting efficiency of Phoebe sheareri tissue culture seedlings, and the rooting efficiency of the Phoebe sheareri tissue culture seedlings is improved through multiple steps of seed disinfection, germination culture, external hormone treatment, active carbon adsorption of secondary metabolites and culture medium optimization, so that the tissue culture efficiency of the Phoebe sheareri is systematically optimized and improved, the survival rate of the Phoebe sheareri tissue culture is improved, and the rooting rate of the Phoebe sheareri is improved from 6.57% to 100%. In addition, the addition of active substances significantly promotes the bolting growth of the Phoebe sheareri, and the bolting rate is increased by about 20% compared with the culture medium without the addition, thereby providing strong support for the subsequent growth of the Phoebe sheareri, especially in the rapid growth of the Phoebe sheareri plant, and providing technical support for commercial application and forestry resource development.

The invention discloses a tissue culture method of root stocks of hemerocallis fulva, which comprises the following steps: step 1, selecting a robust root stock of a hemerocallis fulva variety as an explant, transversely cutting the root stock into small sections, and cleaning and disinfecting the small sections; 2, the disinfected explant is longitudinally cut under the sterile condition and then rapidly transferred to an MS solution containing 0.4-0.6 mg / L of 6-BA and 0.08-0.12 mg / L of NAA to be soaked for 30 min, the temperature of the solution is controlled to be about 20 DEG C, and then the explant is inoculated to an induction culture medium in an upright arrangement mode to induce adventitious buds; step 3, inoculating the adventitious buds on a proliferation culture medium in a single plant or cluster bud mode for proliferation; 4, dividing the adventitious buds into single plants, and inoculating the single plants into a rooting culture medium to induce rooting; 5, after the rooting seedlings are hardened, transplanting is conducted, and hemerocallis fulva plants are obtained. The method applies the double-petal hemerocallis fulva variety, and has the advantages of being capable of obtaining materials all year round, high in propagation coefficient and the like.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The invention provides a fig sapling cultivation method. The fig sapling cultivation method comprises the following steps: S1, selecting a seedling cultivation land; S2, picking cuttings; S3, pruning the cuttings; wherein in the step S5 of rooting treatment of the cuttings, vibration of cell membranes of the fig cuttings can be stimulated through ultrasonic treatment, so that cell metabolism is activated, cell division and proliferation are promoted, and more cell bases are provided for rooting. Meanwhile, ultrasonic treatment can influence synthesis and transportation of growth hormone in the fig cuttings and accelerate transportation of indoleacetic acid in the treatment liquid to rooting parts, so that rooting is facilitated, the survival rate of fig sapling cultivation is increased, and germination of the cuttings is accelerated. According to the method, through refined management and a series of technical optimization, the rooting rate, the survival rate and the germination speed of the fig saplings are remarkably increased.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.

The invention discloses application of PSK to improvement of asexual propagation efficiency of lily scales, belongs to the technical field of plant asexual propagation, and particularly aims to solve the problems that existing lily scale cutting propagation is low in efficiency, slow in budding and rooting and the like. The PSK with the effective concentration (preferably 10 nM <-1 > mu M) is prepared into a treatment solution; during cuttage, the water is applied as first-time irrigation liquid, and the physiological response of the scales can be systematically regulated and controlled. Experiments show that the application can significantly improve the budding rate of longya lilium scales, promote the expansion of new bulblets, and synchronously improve the rooting rate. The invention provides a novel efficient asexual propagation method for lily, which is simple and convenient to operate, remarkable in effect and safe, and is suitable for large-scale production of lily seedlings.