50results about How to "High induction success rate" patented technology

The invention discloses an attack perception method, device and equipment based on honeypot induction and a medium. The method comprises the following steps: filtering a captured data package to obtain a first target data package, and outputting the first target data package to a user mode process; determining a target communication induction strategy corresponding to the first target data packetin a user mode process, and communicating with a source Internet protocol address corresponding to the first target data packet based on the target communication induction strategy to capture at leastone second target data packet sent by the source Internet protocol address; and determining the first target data packet and / or the second target data packet corresponding to the first target data packet as a to-be-analyzed data packet, analyzing the to-be-analyzed data packet based on a preset analysis rule set, and analyzing an attack behavior according to an analysis result. The system has thedual advantages of an intrusion detection system and a honeypot system, attack information can be captured more comprehensively, and the safety of the network is protected.

The invention provides a method for inducing protocorms by utilizing dendrobe tissues. The method comprises the following steps: (1) carrying out routine asepsis on the obtained dendrobe tissues, wherein the dendrobe tissues are from the stem tip, root tip or tender stem section of dendrobe; and (2) carrying out induction culture on the dendrobe tissues subjected to asepsis in an induction culture medium firstly at the temperature of 23-25 DEG C and under a dark condition for one week and then at the temperature of 23-25 DEG C and under an illumination condition for 20-30 days with the illumination period of 12h / day, thus obtaining the protocorms. The method provided by the invention has the following main beneficial effects: (1) the method is simple in operation, low in production cost and less in environmental pollution; (2) the induction success rate is high, wherein the induction rate based on stem section culture is generally about 50%, the induction rate based on root tip culture can reach 60%-70%, and the best induction success rate based on the stem tip culture can reach about 75%; (3) the culture cycle is short, a large number of protocorms can be obtained within less time, and the induced protocorms can be directly subjected to aseptic culture so as to obtain a large number of seedlings; and (4) tissue culture is directly utilized, thus the characters of parents are well maintained.

The invention discloses an establishing method and application of a mouse aortic dissecting aneurysm model. The establishing method of the mouse aortic dissecting aneurysm model is simple and convenient, the mouse aortic dissecting aneurysm model can be established within a short time, surgical operation is not needed, trauma to experimental mice can be reduced, and high operability is achieved. The occurrence rate of aortic dissecting aneurysm of a modeling group is 90%, the breaking rate of the aortic dissecting aneurysm of the modeling group is 20%, the occurrence rate of the aortic dissecting aneurysm in chests and aortic dissection above chests is 90%, the occurrence rate of the aortic dissecting aneurysm in abdomen is 20%, and the induction success rate is high. The dissection occurrence position of the aortic dissecting aneurysm is similar to that of human beings, so that the mouse aortic dissecting aneurysm model can be used for easily studying the pathomechanism of the aortic dissecting aneurysm of human beings and can be applied to suspicious virulence gene screening and treatment research.

The invention discloses a method for cultivating a pearl by inducing triploid hyriopsis cumingii. The method comprises the following steps of: cultivating the triploid hyriopsis cumingii by induction, namely selecting diploid hyriopsis cumingii with age of 3 to 6, shell length of over 15cm and shell thickness of over 5cm in normal growth as parent clams; isolating the male clams from the female clams of the selected parent clams for cultivation respectively; tracking and observing the development condition of the eggs of the female clams; transferring the female clams into a pond for cultivating the male clams to perform natural insemination on the female clams after the eggs are maturely developed; observing the development condition of the fertilized eggs; putting the female clams into 300 to 450 mu mol / L aqueous solution of 6-dimethyl aminopurine after over 80 percent of the fertilized eggs have first polar bodies; and performing induction processing for 10 to 30 minutes to obtain induced female clams; cultivating the female clams, and hatching and raising the seedlings to obtain the triploid juvenile clams; and performing cultivation to obtain the triploid hyriopsis cumingii. In the method, the normal diploid hyriopsis cumingii is used as the parents, and the natural insemination is adopted, so that the induction success rate is improved to over 70 percent.

The invention discloses a fullerene nano-material for preventing and / or treating aplastic anemia and a use thereof. The use of the fullerene nano-material comprises 1, improving a leukocyte generation amount, 2, improving an erythrocyte generation amount, 3, improving a hemoglobin generation amount, 4, improving a platelet generation amount, 5, improving a hematocrit value, 6, improving a mean corpuscular volume, 7, improving mean corpuscular hemoglobin content or 8, improving a mean corpuscular hemoglobin concentration. The fullerene and / or metallofullerene nanometer material can be prepared through a simple and fast method, has good biocompatibility, has no toxic or side effects on organisms and can be used for related hemopoietic tissue through blood circulation. The fullerene and / or metallofullerene nanometer material has a certain protection and / or restoration effect on anaemia and related diseases and has obvious anaemia-related index recovery effects. The fullerene and / or metallofullerene nanometer material has stable properties and can protect and / or repair cells, blood and marrow.

The invention discloses a method for callus of lycoris radiate by using flower stalks, comprising the following steps: taking the flower stalks of the lycoris radiate after being sterilized; inoculating in induction culture medium, cultivating for 28-32 d in sunshine, then moving to a dark environment, and cultivating for 88-92 d, wherein the induction culture medium comprises 2.0-2.5 mg / L of 2,4-D and 1.5-2.5 mg / LBA of MS culture medium, and the MS culture medium comprises sugarcane of which concentration is 30-60 g / L and agar of which concentration is 5-8 g / L and pH is 5.6-5.8. The invention adopts the flower stalks of the lycoris radiate as the exophyte, produces the callus of the lycoris radiate through cultivating in the induction culture medium, cannot be at the cost of consuming the bulbs, and effectively protects the genetic resources of the rare lycoris radiate which has good quality.

The invention discloses a mastoptosis-shaped burl forming method of ginkgo trees by artificial induction. 5-50 years old, strong-root, healthy and pest-free ginkgo trees are used as experimental materials, the mastoptosis-shaped burls of the ginkgo trees are obtained by a carving method so as to meet requirements of academic researching and viewing. According to the ringing and girdling technical theory, downward delivery of organic nutrition (photosynthate) is prevented, nutrition level of branch above wounds is increased, and downward growth of tree trunks is stimulated by nutriment accumulated at the wounds so as to form burls which further grow to be mastoptosis-shaped burls. The mastoptosis-shaped burl forming method has a remarkable effect on inducing to form the mastoptosis-shaped burls, and is easy to operate and feasible.

The invention relates to a polyploid induction method for Chinese gooseberry Hort 16A offspring fine strain tissue culture seedlings. The polyploid induction method comprises the following steps: soaking leaves of the Hort 16A offspring strain tissue culture seedlings in a colchicine water solution to induce polyploidy, washing the leaves, and inoculating in callus differentiation enrichment culture medium and subculture culture medium for light culture according to the steps. The polyploid induction method is simple in operation, good in induction directionality, high in induction success rate, low in cost, shorter in culture period and higher in induction rate, an efficient and feasible new method is provided for polyploidy breeding of Chinese gooseberry, the polyploid induction method has important application values, through the popularization of the polyploid breeding method, economic benefits can be brought for fruit farmers, and demands of more consumers are met.

The invention discloses a method for culturing an autotetraploid paris polyphylla plant, which solves the problem of variety degeneration in natural propagation and artificial planting of paris polyphylla. The method comprises the steps of (1) cultivating tissue culture seedlings; (2) performing colchicines treating, namely performing treatment by one of the following two methods: firstly, selecting a tissue subculture seedling under a germfree condition, forming a plurality of wounds at the stem part, closely covering the tissue subculture seedling by degreasing cotton, dropping a colchicines solution on the degreasing cotton for soaking, performing culture on a differentiation culture medium, cleaning the tissue subculture seedling by germfree water, cutting the tissue subculture seedling into single buds, and transferring the single buds into a rooting induction culture medium; and secondly, transferring the tissue culture seedling into the differentiation culture medium added with colchicines, after culture is performed, cutting the tissue culture seedling into single buds, and transferring the single buds into a cluster bud induction culture medium; (3) performing differentiation culture on the plant; (4) performing multiplication culture on the plant; (5) detecting chromosomes of the plant; and (6) selecting and culturing the autotetraploid plant. The method disclosed by the invention has a great significance on enlarging of the planting area of peculiar valuable medicinal plants.

The invention provides a culturing method for sunflower callus tissue. The culturing method comprises the following steps that after a sunflower seed is soaked, water on the seed surface is absorbed up, and then the sunflower seed is disinfected with chlorine gas; the disinfected seed is inoculated on an aseptic seedling culture medium, and the aseptic seedling culture medium is placed in a constant-temperature dark environment to enable the seed to germinate after inoculation and then transferred to a constant-temperature greenhouse to continue to grow after the seed germinates; cotyledon and true leaves of an inoculated aseptic seedling are selected as explants and placed on an induction medium after the edges of the cotyledon and the true leaves are cut off, the surfaces of the cotyledon and the true leaves are gently pressed with blunt tweezers to enable the cotyledon and the true leaves to be closely attached to the surface of the induction medium, and an opening is sealed with two layers of paraffin wax sealing membranes; after being inoculated, the explants are placed in a constant-temperature biochemical incubator to be cultured until the callus tissue grows out; the callus tissue is cut off and transferred and inoculated into a subculture medium to continue to be cultured until the callus tissue grows to a stable period, and culturing is completed. According to the culturing method, the culturing cycle is shortened by utilizing the cotyledon of the aseptic seedling as the explant and the appropriate hormone concentration.

The invention relates to a Pyrus pyrifolia polyploid induction method. The Pyrus pyrifolia polyploid induction method includes subjecting a single-bud stem segment of a Pyrus pyrifolia tissue culture seedling to polyploid induction with colchicine, performing differentiation culture and rooting culture after induction, and performing polyploid identification. The Pyrus pyrifolia polyploid induction method has the advantages of high induction directionality, low cost and simplicity in operation. The Pyrus pyrifolia polyploid induction method is a novel feasible efficient method for Pyrus pyrifolia polyploid breeding, and has significant application value in promoting artificial induction techniques for Pyrus pyrifolia polyploid in China.

The present invention discloses a pipless fructus momordicae and the cultivation method. The cultivation method is: 1) breeding the tissue cultivation seedling with the male and the female ex-plant of diploid fructus momordicae; 2) doubly introducing the chromosome with the tissue-cultivation successive-transfer seedling; 3) performing the differential cultivation with different parts of the maleand the female plants after introducing treatment; 4) cutting the stem tip and the stem section of the sprout differentially cultivated to perform the gemmulation cultivation; 5) examining the numberof the chromosome of a cultivated entire plant to select the tetraploid plant for performing the root cultivation and the seedling exercise; 6) planting the tetraploid plant and the diploid plant according to the conventional techniques and performing the artificial pollination to get the hybrid seed when in flower; 7) breeding the hybrid seed to be a complete plant and examining the number of the chromosome; selecting the triploid plant to perform the root cultivation and seedling exercise; 8) planting the triploid plant and the diploid plant and performing the artificial pollination on the male diploid plant with the female triploid plant when in flower; the pipless fructus momordicae can be obtained after the fruiting of the female plant.

The invention discloses a method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release. According to the method, diploid misgurnus anguillicaudatus, tetraploid misgurnus anguillicaudatus and paramisgurnus dabryanus are adopted as propagation parents and are used for hybrid fertilized eggs, such that the hybrid fertilized eggs are obtained; on the 5th minute after the hybrid fertilized eggs are obtained, the hybrid fertilized eggs are soaked in ice water with a temperature of 2-3 DEG C for cold shock treatment with a duration of 30-35min; and the eggs are hatched and cultured under room temperature, such that misgurnus anguillicaudatus allopolyploids are obtained. With the method, allotriploid misgurnus anguillicaudatus, allotetraploid misgurnus anguillicaudatus and allopentaploid misgurnus anguillicaudatus can be obtained. With the method, the induced misgurnus anguillicaudatus allopolyploid variety number is high, and the amount is high. The induction success rate is high, and the offspring growth is fast. With the method, the weight of 12-month-age misgurnus anguillicaudatus is increased by an average of -19.99% to 142.34% than that of a normal selfing control group. The weight gain effect is significant. The method has high popularization benefit.

The invention provides a preparation method of high-purity paclitaxel. The preparation method comprises the following steps: (1) establishing a stable taxus chinensis forming layer stem cell culture system; (2) adding taxus chinensis endophytic fungi extract liquid into a suspension culture medium, inoculating taxus chinensis cells, performing the ventilation culture for 6 to 8 days under an artificial lighting condition, and adding jasmonic acid methyl ester and a clinacanthus nutans extract into the liquid suspension culture medium to continue the culture; (3) collecting the taxus chinensiscells, and performing the coarse extraction of paclitaxel; (4) purifying a crude extract; and (5) enabling a concentrated solution II to pass through a C18 high-performance liquid phase chromatographic purification column, eluting by using methanol, collecting and combining paclitaxel elution parts, decompression evaporating the solvent methanol, crystallizing and releasing the paclitaxel, centrifugally collecting crystals, collecting and combining the paclitaxel elution part, decompression evaporating the solvent methanol to crystallize and precipitate the paclitaxel, centrifugally collectingthe crystals, and vacuum freeze drying the crystals, thus obtaining the high-purity paclitaxel concentrated solution II. The preparation method has the characteristics of high induction success rate,high paclitaxel extraction purity and the like.

The invention relates to the technical field of establishment of an animal model used for screening drug and researching disease mechanism, in particular to a screening method of a febrile convulsion model rat, which can be used for obtaining the model rat by screening in a way of combining a typical water bath heating induction model with artificial directional selection and mating breeding. Themethod is unique and can be used for successfully obtaining the good febrile convulsion sensitive model rat and a tolerance model rat.

The invention relates to the technical field of preparation of medical and biological experiment models and in particular relates to a method for constructing an epilepsy animal model and application.By a specific animal Micl9 knockout mode, an animal model of automatic epilepsy is constructed. The epilepsy animal model constructed by using the method provided by the invention has good stability,low individual differences and high induction success rates.

The invention provides a culture medium for separating and inducing human adipose stem cells into pancreatic beta cells and a use method of the culture medium. The optimal culture medium is designed; gene transfection is not required, so that the risk of gene modification and cancers is avoided, few induction steps are used, the use is simple, and the induction time is short and is shortened by 30% in comparison with the prior art; the culture medium is safe, nontoxic and high in success rate of induction. Moreover, after autogenous adipose mesenchymal stem cells are induced and differentiated into insulin-secreting cells, rejection and ethical problems are avoided after autotransplantation, and the safety is high, so that the clinical application prospect is wide. The culture medium is simple, feasible, low in cost and good in use effect.

The invention provides a method for culturing in vitro tissue of populuseuphratica. The method comprises steps as follows: a stem provided with a bud of the populuseuphratica is inoculated to an initiation culture medium for initiation culture so as to obtain an axillary bud of the populuseuphratica; the axillary bud of the populuseuphratica is transferred to a subculture medium for subculture so as to obtain a populuseuphratica seedling; and the populuseuphratica seedling is transferred to a rooting medium for rooting culture so as to obtain a tissue culture seedling of the populuseuphratica. With adoption of the method, the vitro tissue of the populuseuphratica can be cultured effectively, the induction success rate and the final rooting rate are quite high, so that a large quantity of tissue culture seedlings of the populuseuphratica can be obtained in a short time, and the method is simple and convenient to operate and is suitable for large-scale and fast populuseuphratica reproduction.

The invention relates to the technical field of plant in-vitro culture, in particular to a tissue culture method of a brunnera macrophylla "Hadspen Cream". The tissue culture method comprises the following steps of obtaining and disinfecting explants, inducing the explants to form calluses, inducing the calluses to form adventitious buds, proliferating the adventitious buds and inducing the adventitious buds to root; and in the step of obtaining and disinfecting the explant, a rachis of a mother plant which grows strongly and is free of plant diseases and insect pests is selected as the explant, disinfection treatment is conducted on the rachis, and the disinfected explant is obtained. According to the tissue culture method of the brunnera macrophylla "Hadspen Cream", the propagation efficiency of the brunnera macrophylla "Hadspen Cream" can be improved, meanwhile, the limitation of seasons on the production of the variety is reduced, so that the application of the brunnera macrophylla"Hadspen Cream" is promoted.

The invention provides an induction method for autotetraploids of pomegranates. The induction method comprises the following steps: (1) harvesting pomegranate fruits matured by selfing, taking seeds, cleaning with clean water, drying in the sun, treating the dried seeds in the condition with the temperature of 4 DEG C for 4 days, then putting into a calorstat with the temperature of 27 DEG C and germinating to be in a budding state; (2) soaking the budding pomegranate seeds obtained in the step (1) by using a colchicine solution with the mass-percentage concentration of 0.3%, simultaneously putting the pomegranate seeds on a sunshine-shading constant-temperature swinging bed with the temperature of 25 DEG C, carrying out oscillatory induction culture for 24 hours at speed of 100r / min, and using tap water to soak and clean for 3-5 times with 5 minutes for each time after treatment. The induction method provided by the invention has the advantages that the defect of tedious operation of the general ploidy-induction method is overcome, the operation is simple and easy, the success rate of induction is high, and the need for seed selection of polyploidy is met, so that the polyploidy breeding becomes an important method for genetic improvement and new-variety breeding of the pomegranates.

The invention relates to application of a gama-aminobutyric acid (GABA) translocator isoform I gene knockout mouse system in building a novel drug induced epilepsy animal model. According to the invention, a GAT1 gene knockout mouse is adopted to build an animal epilepsy model, the effect is compared with modeling by adopting wild type mice, and the method provided by the invention is proved to be with high success rate, high epilepsy level, stable state, difficulty in death and good repeatability, and can well meet the requirement of epileptic disease research. The result provided by the invention also proves that after GAT1 gene knockout, the mouse epilepsy susceptibility is increased, the GAT1 gene or protein can be used as a therapeutic target for epilepsy, and the level of the GAT1 gene or protein can be used as a biomarker for epilepsy diagnosis.

The invention discloses a hepatocyte culture solution which comprises dexamethasone with the final concentration of 1-20 [mu] M, curcumin with the final concentration of 1-20 [mu] M and a basic culture medium, the hepatocyte culture solution has the advantages of being simple in component and low in cost, hepatocytes with enhanced functions are obtained through quantitative induction, and application of the hepatocytes to treatment of liver diseases is accelerated. According to the culture method for enhancing the functionality of the hepatocytes and the used hepatocyte culture solution, sufficient nutrition can be provided for growth of the target hepatocytes, and expression of related metabolic enzymes and albumin genes of the target cells can be induced.

The invention relates to the field of stem cell organoid research, and discloses a method for separating crypts from colonic mucosa tissues and inducing to generate colonic organoid. Compared with the prior art, the method has the following advantages and effects that the elastin is degraded through Dispase, the connection between the colon epithelium and the intrinsic layer is loosened on the premise that cells are intact, the three micromolecules of Thiazovivin (TZV), SB431542 and CHIR99021 are added into the OGM culture medium to improve the organoid induction success rate, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. The human colon-like organs can be reliably obtained with relatively low cost and relatively high efficiency; meanwhile, the method has the advantages that the method does not depend on a high-skill epithelial layer stripping technology; more primary colonic progenitor cells are obtained; the induction success rate of the primary culture medium on the colon-like organs is improved; and popularization and application are facilitated.

A related fritillaria anhuiensis callus induced propagation method comprises the following steps: cutting a fritillaria anhuiensis bulb at the growth period into small diamonds, inoculating to an induction medium to form callus through induction, and then inoculating to a propagation medium for propagation and further for obtaining compact callus. The induction medium employs MS medium as a basal medium and contains 6-BA and NAA. The provided method is simple and practicable, shortens the induction period of callus, improves the induction success rate, and has the callus rate of 70-83.33% and the embryogenic callus rate of 80.95-84%. Also, the callus obtained according to the provided induced propagation method has relatively good quality and growth seedling-forming performance.

The invention relates to the technical field of preparation of medical and biological experimental models, in particular to a construction method and application of an epilepsy animal model. An animal model of spontaneous epilepsy was constructed by specifically knocking out Mic19 in animals. The epilepsy animal model obtained by adopting the construction method of the present invention has better stability, lower individual differences, and high induction success rate.

The invention relates to a preparation method for simply inducing bacteria to form SCVs in a laboratory, the induction success rate is high, and the problems that the SCVs induction workload is large, and the SCVs are easily covered by wild strains and are difficult to separate and purify are solved. The method can greatly simplify the induction and purification procedures of the bacteria SCVs and improve the induction screening efficiency. According to the preparation method disclosed by the invention, the continuous drug gradient concentration taking the filter paper as the center is formed on the solid culture medium, and various antibiotic filter paper can be placed on the same solid culture medium, so that the purpose of inducing and screening the same strain by using various antibiotics at the same time is achieved, and the working efficiency is greatly improved. The resistance of SCVs to antibacterial drugs is increased, so that the selection difficulty of clinical antibacterial drugs is improved. The escherichia coli SCVs are successfully separated and identified, a biological material is provided for research on SCVs related diseases, and a basis is also provided for diagnosis, treatment and prevention of escherichia coli SCVs related diseases.

The invention particularly relates to a method for inducing tumor cells to form tumor stem cells. The specific method comprises the following steps: performing synchronization treatment on tumor cells in a logarithmic phase, inoculating the tumor cells in a suspension state into a tumor stem cell induction culture medium, periodically applying mechanical force at fixed time intervals to suspend, dispersing the adherent tumor cells, and continuing to culture. According to the method disclosed by the invention, the adhesion capacity of the cells is reduced through the methods of synchronous serum-free treatment, suspension state inoculation and periodic suspension dispersion, so that the cells directly enter a suspension state, and the screening process of the dry tumor cells is completed only by using a common culture dish, a pipette, a centrifugal machine and the like; high-quality experiment results are achieved, meanwhile, limitations of valuable consumables, advanced equipment and complex technologies are broken through, and experiment cost, equipment limitations and technical thresholds are reduced.

The invention relates to the field of plant breeding, in particular to a centella asiatica polyploid and a method for inducing breeding thereof. The method comprises the following steps: transplanting wild centella asiatica to the culture medium; preparing an inducer solution; the inducer solution has a concentration of 30-50 mg / L amisulfame, and is added with 1-3 wt % dimethyl sulfoxide ; Wrap the absorbent cotton on the growing point of Centella asiatica cluster leaves, soak the absorbent cotton with inducer solution; treat it under natural light conditions for 2 to 4 days; use asexual reproduction to expand and preserve the samples that are tetraploid detected by chromosome microscope. The invention conducts in vivo induction of Centella asiatica, not only has a high induction transformation rate, but also greatly increases the content of medicinal active substances in the multi-volume Centella asiatica.

The invention discloses an induction culture medium of an autograph tree and a vegetative propagation method for the autograph tree. According to the induction culture medium of the autograph tree, anMS culture medium serves as a basal culture medium, and 6-benzylaminopurine, naphthylacetic acid, silver nitrate, activated carbon, manganese dioxide, hydrolyzed casein, agar powder and white granulated sugar are added. The vegetative propagation method comprises the steps of firstly, carrying out disinfecting treatment on an explant, then, carrying out slight scraping treatment and scratching treatment, and finally, carrying out inoculating. The induction culture medium of the autograph tree and the corresponding vegetative propagation method, disclosed by the invention, can be used for promoting growth and development of tender leaves of the autograph tree, shortening induction time, reducing a browning phenomenon and increasing the survival rate of induction of the tender leaves of theautograph tree.