Method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release

An allopolyploid and tetraploid loach technology, applied in fish farming, application, climate change adaptation, etc., can solve the problem of low induction success rate, achieve obvious weight gain effect, easy access to materials, and strong promotion effect of benefit

Inactive Publication Date: 2015-03-11
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1969616A discloses a method for inducing hybrid allotetraploids of bream and triangular bream to obtain an allotetraploid population of bream, but the induction success rate is not high
At present, there are no reports at home and abroad on the systematic research on the induction of loach allopolyploidy

Method used

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  • Method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release
  • Method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release
  • Method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release

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Embodiment 1

[0017] The establishment of embodiment 1 optimal induction condition

[0018] The invention utilizes a physical method (cold shock treatment) to induce the allopolyploid loach, and mainly obtains the allopolyploid loach by inhibiting the release of the second polar body of the fertilized egg. According to the principle of three-factor experimental design, the experimental conditions (shock start time, shock temperature and shock treatment time) were designed in gradient respectively. The shock start time was the 4th, 5th and 6th minutes after fertilization respectively; 1°C, 2°C, 3°C, 4°C; the duration of shock treatment was 25 minutes, 30 minutes, and 35 minutes, respectively. Two parallels were designed for each experimental group, and the appropriate induction conditions were determined for subsequent experiments by counting the survival rate of embryos and the success rate of polyploid induction.

[0019] The preferred start time of shock in the present invention is 5 min...

Embodiment 2

[0020] The establishment of embodiment 2 loach allopolyploid

[0021] First, wild loach was caught from the waters around Wuhan City, Hubei Province. Select well-developed, disease-free and non-injured as breeding parents. In the laboratory, flow cytometry (Beckman, Quanta SC) was used to identify the DNA content of blood cells to determine their ploidy. By injecting oxytocin drugs to the breeding parents, the oxytocin drugs used are ovulation-inducing hormone No. 2 (LRH-A2) and dioconone (DOM). Half, the injection method is a single intraperitoneal injection. After the injection of oxytocin drugs, the male and female fish were kept separately in different water tanks, and the water temperature was 24-26°C. 12-15 hours after the injection of oxytocin drugs, dissect the male fish to take out the semen, and mix the testis and sperm preservation solution (preservation solution formula: sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, glucose 3.0g, distille...

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Abstract

The invention discloses a method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release. According to the method, diploid misgurnus anguillicaudatus, tetraploid misgurnus anguillicaudatus and paramisgurnus dabryanus are adopted as propagation parents and are used for hybrid fertilized eggs, such that the hybrid fertilized eggs are obtained; on the 5th minute after the hybrid fertilized eggs are obtained, the hybrid fertilized eggs are soaked in ice water with a temperature of 2-3 DEG C for cold shock treatment with a duration of 30-35min; and the eggs are hatched and cultured under room temperature, such that misgurnus anguillicaudatus allopolyploids are obtained. With the method, allotriploid misgurnus anguillicaudatus, allotetraploid misgurnus anguillicaudatus and allopentaploid misgurnus anguillicaudatus can be obtained. With the method, the induced misgurnus anguillicaudatus allopolyploid variety number is high, and the amount is high. The induction success rate is high, and the offspring growth is fast. With the method, the weight of 12-month-age misgurnus anguillicaudatus is increased by an average of -19.99% to 142.34% than that of a normal selfing control group. The weight gain effect is significant. The method has high popularization benefit.

Description

technical field [0001] The invention relates to the fields of fish crossbreeding and polyploid breeding, more specifically, it relates to a method for obtaining allopolyploid loach by inhibiting the release of fertilized egg polar bodies. Background technique [0002] Loach (Misgurnus anguillicaudatus) and paramisgurnus dabryanus (Paramisgurnus dabryanus) are two important freshwater economic loach species in my country. In 2011, the output of these two species exceeded 230,000 tons. The two kinds of loaches are distributed in other parts of the country except the Tibet Plateau of my country. In recent years, due to the sharp reduction of natural resources, wild-caught loach is far from meeting the needs of the market, and it is imminent to carry out loach breeding and the task of breeding fine varieties. In fish breeding work, two commonly used techniques include: hybrid breeding and polyploid breeding. The offspring of hybrid breeding can obtain the advantages of inherit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 黄松钱曹小娟田先畅王卫民
Owner HUAZHONG AGRI UNIV
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