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Onion callus induction method and special culture medium thereof

A technology of callus induction and callus, applied in the field of plant tissue culture, can solve problems such as no practical application, and achieve the effects of increased induction rate, low pollution rate, and simple and convenient disinfection and sterilization methods

Inactive Publication Date: 2011-02-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is still no practical application

Method used

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  • Onion callus induction method and special culture medium thereof
  • Onion callus induction method and special culture medium thereof
  • Onion callus induction method and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.1 Obtaining sterile materials

[0026] The onion material used in the experiment was taken from the experimental base of the College of Horticulture, Nanjing Agricultural University, and the variety was "9866". Use young inflorescences with a diameter of about 0.8-1.2cm, after tearing off the involucre, cut it into 6-8 small pieces together with the inflorescence axis as explants, numbering A; For the uncracked inflorescence, the young bud with a short stalk (0.1-0.3cm) is used as the explant, number B; for the inflorescence with a diameter of about 3.0cm that has been cracked by the involucre, whichever has a short stalk (0.1-0.3cm) young buds are explants, coded C.

[0027] Sterilize the inflorescence first, then tear off the involucre, and take explants A and B respectively. The young buds with short stalks were taken and sterilized to obtain explant C. For disinfection and sterilization, first rinse with tap water for 0.5h, rinse to remove impurities, disinfect...

Embodiment 2

[0047] Medium formula: B5 with a sucrose concentration of 30g / L, an agar concentration of 8g / L, and a pH value of 5.8 was used as the basic medium, and 1.0mg / L 2,4-D and 1.0mg / L 6-BA were added.

[0048] Take young onion inflorescences with a diameter of about 1 cm, disinfect and sterilize (first rinse with tap water for 0.5 h, rinse off impurities, disinfect with 70% alcohol for 1 min, then disinfect with 0.1% mercuric chloride for 10 min, and rinse with sterile water for 3 min. ~5 times.), after tearing off the involucral integument, cut it into 6-8 small pieces together with the inflorescence rachis as explant A, and inoculate the obtained sterile material explant A on the medium.

[0049] Place the inoculated petri dish at a temperature of 25±1°C and a light time of 16h.d -1 , with an illumination intensity of 50 μmol m -2 ·s -1 cultivated under conditions.

[0050] It can be observed that after inoculation, the base of the inflorescence rachis and the young buds grow u...

Embodiment 3

[0052] Medium formula: B5 with a sucrose concentration of 30g / L, an agar concentration of 8g / L, and a pH value of 5.8 was used as the basic medium, and 1.0mg / L 2,4-D and 1.0mg / L 6-BA were added.

[0053] Take the uncracked inflorescences of the involucre with a diameter of about 1.5-2.0 cm, and sterilize (wash with tap water for 0.5 h first, rinse off impurities, sterilize with 70% alcohol for 1 min, then sterilize with 0.1% mercuric chloride for 10 min, then Rinse 3 to 5 times with sterile water.), after tearing off the involucre, take the young bud with a short stalk (0.1-0.3cm) as explant B, and the obtained sterile material explant B inoculated on the medium.

[0054] Place the inoculated petri dish at a temperature of 25±1°C and a light time of 16h.d -1 , with an illumination intensity of 50 μmol m -2 ·s -1 cultivated under conditions.

[0055] It can be observed that the growth is slow after inoculation, some flower buds are opened, and a yellowish callus appears aft...

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Abstract

The invention belongs to the field of plant tissue culture, and discloses an onion callus induction method and a special culture medium thereof. The onion callus induction method comprises the following step of: carrying out callus induction culture on an onion explant by using a B5 culture medium added with 2,4-D and 6-BA, wherein the sucrose concentration of B5 culture medium is 30g / L and the agar concentration of B5 culture medium is 8g / L, the pH value of culture medium is 5.8, the added 2,4-D concentration is 0.5-2.0mg / L, and the 6-BA concentration is 1.0-4.0mg / L. The selected onion explant has the advantages of simple and convenient disinfection and sterilization method, and low pollution rate after inoculation and high callus induction rate.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to an onion callus induction method and a special culture medium thereof. Background technique [0002] Onion belongs to Liliaceae (Liliaceae) Allium genus, and is one of the important vegetables widely cultivated in the world. It forms product organs with fleshy scales and scale buds, and is rich in nutrients. It is an important nutritional and health-care vegetable. In recent years, my country's onion cultivation area has expanded rapidly, and onions and their processed products have been exported to Europe, America, Japan and many countries in Southeast Asia. However, most of the varieties used in my country's onion production are foreign varieties, and there are few domestic onion varieties. The reason for this situation is that onions are biennial herbaceous plants with small flower structures, which are prone to degeneration after self-breeding, and there are long breeding cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王建军刘照坤侯喜林
Owner NANJING AGRICULTURAL UNIVERSITY
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