Onion callus induction method and special culture medium thereof

Abstract

The invention belongs to the field of plant tissue culture, and discloses an onion callus induction method and a special culture medium thereof. The onion callus induction method comprises the following step of: carrying out callus induction culture on an onion explant by using a B5 culture medium added with 2,4-D and 6-BA, wherein the sucrose concentration of B5 culture medium is 30g / L and the agar concentration of B5 culture medium is 8g / L, the pH value of culture medium is 5.8, the added 2,4-D concentration is 0.5-2.0mg / L, and the 6-BA concentration is 1.0-4.0mg / L. The selected onion explant has the advantages of simple and convenient disinfection and sterilization method, and low pollution rate after inoculation and high callus induction rate.

Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and relates to an onion callus induction method and a special culture medium thereof. Background technique

[0002] Onion belongs to Liliaceae (Liliaceae) Allium genus, and is one of the important vegetables widely cultivated in the world. It forms product organs with fleshy scales and scale buds, and is rich in nutrients. It is an important nutritional and health-care vegetable. In recent years, my country's onion cultivation area has expanded rapidly, and onions and their processed products have been exported to Europe, America, Japan and many countries in Southeast Asia. However, most of the varieties used in my country's onion production are foreign varieties, and there are few domestic onion varieties. The reason for this situation is that onions are biennial herbaceous plants with small flower structures, which are prone to degeneration after self-breeding, and there are long breeding cy...

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