1785 results about "Plant tissue culture" patented technology

This invention describes a simple method useful for the excision and isolation of maize immature embryos. The embryos are useful for plant tissue culture and transformation methods.

Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

The invention relates to a method for the plant tissue culture, in particular to the tissue culture method and application of the aoectochilus formosanus. The invention provides a culture method of aoectochilus formosanus tissue culture seedling comprising the steps that: the induction culture is performed by taking the wild aoectochilus formosanus as the raw material, then by means of hormone-free culture, the asepsis culture line of the aoectochilus formosanus tissue culture seedling is obtained; after that, the tissue culture seedling system which is suitable for a large-scale production is established, while the essential effective composition (amylase, amino acid) of the culture is close to the wild aoectochilus formosanus and stable. The main content comprises the configuration of the tissue culture seedling medium; the culture conditions of the tissue culture seedling; and the planting technology and usage of the tissue culture seedling. The invention solves the problem of the lack of natural resources of the wild aoectochilus formosanus.

A method for quickly reproducing Mandiya yew is based on the tissue culture principle plus the cuttage technique includes such steps as taking part of the organs of said yew, treating it by the invented "tonic G-P clone liquid", planting in sand bed, supplementing said "tonic G.P clone liquid" while management, and growing root in 20 days. Its advantages are high speed and survival rate and low cost.

The invention discloses a fast tissue culture reproducing method of actinidia eriantha, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) tissue culture medium preparation; (2) explant selection and sterilization; (3) inducing culture; (4) proliferation culture; (5) rooting culture; (6) tissue culture plantlet acclimatization and transplant, and the like. The fast tissue culture reproducing method has high culture medium pertinence and good applicability, the inductivity of an initial sprout of an explant reaches more than 80 percent, the proliferation rate of a successive sprout reaches 2-7 times, the plantlet height can reach 2-5 centimeters for 20-30 days, the quality of tissue culture plantlets is enhanced, the rooting rate reachesmore than 95 percent, and the transplant survival rate reaches more than 95 percent. The invention can be popularized and applied to fruit tree plantlet enterprises.

The invention discloses a Dendrobium in vitro crossbreeding method. The method can be used to greatly shorten the maturation period of fruits, to enable a hybrid to bloom in vitro in a short period so as to observe flower shapes and colors, and to cultivate a novel variety, thus accelerating Dendrobium breeding, the first such report internationally. Since in vitro Dendrobium blooms annually, it enables crossbreeding of varieties having different inflorescences in nature. In addition, the medium used at each stage of the invention utilizes Hyponex which has a unique composition and costs little, thus allowing for a high blossoming rate and rapid fruit development. Also, only simple plant tissue culture equipment is required for implementing the invention, thus the entire breeding method is simple and low cost, and provides conditions for cultivating of high-quality Dendrobium varieties.

The invention relates to the technical field of microbes, and discloses a separation method for plant endophyte. The separation method comprises the following steps: (1) pretreating plant tissue; (2) culturing plant tissue; and (3) separating endophyte. The disclosed separation method for plant endophyte is capable of effectively removing surface bacteria, fungi, actinomyces and other microbes, and avoiding endophyte separation effect caused by mixing of mixed bacteria. Endophyte in the plant tissue recovers growth after tissue culture, and disinfection processing does not need performing in operation of separating endophyte, so that the damage effect of a disinfectant on plant tissue and endophyte is substantially reduced, the survival probability of endophyte is increase, and the number of endophyte obtained through separation is obviously improved.

The fast propagation method of hemp for industrial use is one hemp tissue culture process including the steps of sterilizing seed, obtaining bacteria-free seedling, culturing lateral bud and rooting culture. The hemp tissue culture process has no limitation of seasons, saving in seedling culture field, low production cost, capacity of maintaining all the excellent characteristics of the mother plant and stability of the genetic characteristics. The present invention can form great amount of tube seedling in short period for mass planting of hemp.

The invention discloses a plant tissue culture environmental information monitoring and simulating system. The plant tissue culture environmental information monitoring and simulating system comprises a tissue culture box, a monitoring unit and a simulating unit, wherein the monitoring unit is formed by a collection module, a wireless transmission module and a display processing module. The collection module collects tissue culture environmental information of plants in the tissue culture box, and sends the environmental information to the transmission module. The wireless transmission module uploads the received environmental information to the display processing module. The display processing module stores, processes, displays and indexes the environmental information. The simulating unit carries out manual simulation on the tissue culture environmental information of the plants in the tissue culture box. The plant tissue culture environmental information monitoring and simulating system is convenient to operate, complete in functions, strong in pertinence, capable of achieving intelligent control for the information such as illumination, temperature, carbon dioxide (CO2) and the like in a greenhouse environment, and is suitable for greenhouse plant tissue culture and for various places such as plant tissue culture boxes, illumination culture boxes, phytotrons, facility agriculture, and has expansibility.

The invention provides a method for propagating stonegarlic quickly and efficiently. The method is used for large-scale production and propagation, and then market requirements are met. The stonegarlic tissue culture comprises steps of A, sterilization of explants, B, inducement of adventitious buds, C, proliferation of adventitious buds, D, rooting culture and E, seedling exercise and transplanting. The stonegarlic is propagated through the method of plant tissue culture, then the propagation cannot be affected by external conditions and can be performed at all seasons, lands occupied by seedlings are saved, the production cost is saved, all excellent characters of a matrix can be preserved, and hereditary characters are stable. By the aid of the method, a lot of excellent test-tube plantlets can be formed in a short time, the scale and industrial production is performed, and industries such as ornamental horticulture and pharmacy can obtain a lot of raw materials.

The invention discloses a culture method of high diplont rate sporule regeneration plant of broccoli, which belongs to the technical field of plant tissue culture and comprises the following steps: (1), preparing a culture medium; (2), culturing the high diplont rate sporule regeneration plant of broccoli: 1), selecting a donor plant and a bud, 2), sterilizing the bud, 3), pre-treating and culturing the bud, 4), separating, mixing and subpackaging of bud sporule, 5), culturing sporule embryoid, 6), differentiating and culturing a regeneration plant, 7), rooting and transplanting the regeneration plant and 8), detecting ploidy of the regeneration plant. The invention obviously increases the germ extraction rate and the rate of emergence of the sporule culture of the broccoli and the diplont rate of the regeneration plant respectively to 120 embryos/bud, 70 percent and more than 70 percent from common 80 embryos/bud, 50 percent and about 50 percent, thereby improving the breeding efficiency of the broccoli. The method can be popularized and applied to a vegetable breeding department or company.

The invention mainly relates to the field of plant tissue culture and particularly relates to a butterfly orchid anti-browning tissue culture method and an anti-browning culture medium. The tissue culture method comprises the steps of explant disinfection, primary culture, proliferation and differentiation culture, rooting culture, seedling hardening, transplanting and the like, wherein in the primary culture and the proliferation and differentiation culture, extracted solutions of calendulas, lemons, tomatoes, rosemary and the like are added into a corresponding culture medium so as to effectively prevent and treat a common browning problem in butterfly orchid tissue culture; and meanwhile, different types of cutting manners are used for helping to prevent and treat the browning problem at a differentiation phase, so as to form a compound anti-browning method. The method disclosed by the invention has the advantages of high stability, great success rate, greenness and environmental friendliness, and can annularly and repeatedly produce.

Provided is a plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide. The bacteriostatic (antiseptic) agent comprises isothiazolinone, nisin and aqueous extracts of the Chinese herbal medicines of terminalia, groundsel and aromatic madder. When the bacteriostatic (antiseptic) agent is applied in plant tissue culture, 0.35 to 0.5% of the bacteriostatic (antiseptic) agent is added into a medium, which enables fungal and bacterial contamination to a culture to be effectively reduced or prevented and poses little adverse influence on plant growth, thereby enhancing the success rate of plant tissue culture; the bacteriostatic (antiseptic) agent can also be applied in open type plant culture, e.g., in the rooting phase of sound seedlings during a late plant culture stage, the bacteriostatic (antiseptic) agent is directly added into a medium, and the medium can be inoculated in indoor environment with bacteria or a few bacteria without undergoing the step of autoclaving or operating on a superclean bench, thereby simplifying seedling culture in plant tissue culture, enhancing working efficiency, saving a great amount of energy and reducing production cost for plant tissue culture seedlings; therefore, the bacteriostatic (antiseptic) agent has a critical actual application value.

The invention discloses a composition for preventing browning of a plant tissue culture. The composition contains a direct antioxidant against phenolic substances and a plant antioxidase system reinforcing agent, and can play the systematic synergic action of two anti-browning mechanisms during the plant tissue culture process, on the one hand, the anti-oxidation action of the anti-oxidant can be realized against the phenolic substances infiltrating into a culture medium so as to reflect the direct reduction action, on the other hand, the plant antioxidase system reinforcing agent can reinforce the activity of antioxidase in strawberry tissues in plant tissues, and the production and the expansion of basic factors of the browning, namely quinone type substances can be reduced; and the combination of the two can effectively prevent the browning phenomenon in primary culture of strawberries and reduce the browning rate from the conventional level of above 30% to below 20%. The composition disclosed by the invention is widely suitable for various plants which are prone to browning in different tissue culture stages. The invention further discloses a using method of the composition.

The invention discloses a tissue-culture quick breeding method for iris tectorum, belonging to the technical field of plant tissue culture. The method comprises the following technical steps: (1) preparing a culture medium; (2) culturing iris tectorum detoxified tissue-culture seedlings; and (3) hardening and transplanting the tissue-culture seedlings. With the method, through adjustment on the type and the content of culture medium hormones, addition of antibiotics and other technical measures, the pollution rate of a iris tectorum explant is obviously lowered, and an inoculation survival rate is above 90%; the induction differentiation rate of the explant can be obviously improved to 99% from 70-80% in the prior art; and the tissue-culture strong seedling rate of the iris tectorum is improved. The tissue-culture quick breeding method can be popularized and applied to landscaping and tissue culture enterprises.

The invention relates to a method for producing the lycoris by plant tissue culture, which comprises the steps of: (1) taking an MS (mass spectrometry) culture medium as a basic culture medium, and taking a 2, 4-D, IAA (indo acetic acid), NAA (naphthyl acetic acid), 6-BA (butyl acrylate) and IBA (iso butyl alcohol) as a regulated and controlled growth hormone preparation culture medium; (2) taking the bulb of the lycoris as an explant, and sterilizing; (3) vaccinating the explant of the bulb on a bulb inducing culture medium to culture in an inducing way so as to grow bulbils; (4) transferring the bulbils into a rooting culture medium to culture in a rooting way, so that the bulbils can independently absorb the nutrient elements from the soil and the matrix; (5) transferring the bulbils into the culture medium to culture; (6) opening an opening of a vaccination bottle to refine the seedling; withdrawing the bulbils, and transferring the bulbils into a nutrient cup which takes the vermiculite, the pearlite and the nutrient soil as the mixed matrix; and transferring the bulbils into a large tent, so that the survival rate reaches 94%-96%. The method is less in material taking, and economical in culturing; the culture condition can be manually controlled and is not influenced by the natural condition; the method is short in growing period, and high in propagation rate; and the quality of lycoris is guaranteed.

The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.

The invention belongs to the technical field of plant tissue culture and particularly relates to a high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture. Primary inducing culture medium used in the method is improved WPM+Zt 1.0-1.5mg/L, successive multiplication culture medium is improved WPM+Zt 0.5-0.8mg/L, and rooting culture medium is 1/2 improved WPM+IAA 0.1-0.2mg/L+AC 0.5g/L. The method comprises the steps of: dipping the stem bases of successively multiplied aseptic blueberry seedlings in IBA sterile solution with concentration being 100-500mg/L for 5-15 seconds, inoculating the stem bases in the blueberry rooting culture medium, moving a rooting tissue culture seedling culture bottle from a culture room to a plastic greenhouse for training seedlings for 4-7 days after rooting, uncovering the plastic greenhouse and training the seedlings for 3-5 days before transplanting, transplanting the seedlings in culture medium in a cell tray, laying a plastic film to preserve moisture for 5-7 days, ventilating twice a day for 5-15 minutes per time during moisture preservation and fully removing the plastic film after 7 days. The high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture has the advantages that not only is the efficiency high, but also the cost is lower and the mass production is facilitated.