1787 results about "Plant tissue culture" patented technology

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

This invention describes a simple method useful for the excision and isolation of maize immature embryos. The embryos are useful for plant tissue culture and transformation methods.

Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

The invention relates to a method for the plant tissue culture, in particular to the tissue culture method and application of the aoectochilus formosanus. The invention provides a culture method of aoectochilus formosanus tissue culture seedling comprising the steps that: the induction culture is performed by taking the wild aoectochilus formosanus as the raw material, then by means of hormone-free culture, the asepsis culture line of the aoectochilus formosanus tissue culture seedling is obtained; after that, the tissue culture seedling system which is suitable for a large-scale production is established, while the essential effective composition (amylase, amino acid) of the culture is close to the wild aoectochilus formosanus and stable. The main content comprises the configuration of the tissue culture seedling medium; the culture conditions of the tissue culture seedling; and the planting technology and usage of the tissue culture seedling. The invention solves the problem of the lack of natural resources of the wild aoectochilus formosanus.

A method for quickly reproducing Mandiya yew is based on the tissue culture principle plus the cuttage technique includes such steps as taking part of the organs of said yew, treating it by the invented "tonic G-P clone liquid", planting in sand bed, supplementing said "tonic G.P clone liquid" while management, and growing root in 20 days. Its advantages are high speed and survival rate and low cost.

The present invention relates to an open type plant tissue seedling cultivation method pertaining to the agroforestry field of plant tissue seedling cultivation technology. The method can be performed in a nonsterile natural environmental condition in a room temperature and the method comprises steps of adding plant extracts bacteriostatic agent in a culture medium, in each 1L culture medium, adding bacteriostatic agent of 4-15g, manufacturing a bacteriostasis culture medium, and performing an inoculated culture by utilizing the bacteriostasis culture medium. The bacteriostatic agent comprises two kinds one of which comprises garlic extracts, coptis root extracts and honeysuckle leaf extracts with a mass percent of 20-100%:0-40%:0-50%; the other one of which comprises liquorice root extracts, osmanthus extracts and lycoris radiata extracts with a mass percent of 10-100%:0-80%:0-50%. By utilizing the method of the present invention, the plant tissue seedling cultivation process can be divorced from a stringent axenic operation environment and can be performed in an open nonsterile environment, therefore, links in the plant tissue seedling cultivation are simplified radically, the working efficiency is improved and the breeding cost is greatly reduced.

The invention belongs to the fields of development and utilization of plant growth regulating substances, and discloses a hydrogen-rich liquid plant growth regulator. The saturation of hydrogen in a solvent is 0.1 to 100 percent, and the solvent is water, a 25 to 100 percent Hoagland nutrient solution, a Kimura B nutrient solution, a TAP nutrient solution or an MS culture solution. Calcium salt, salicylic acid, salicylate, salicylic acid derivative, humid acid or humic acid salt at the final concentration of 0-1,000mu mol / L is added into the solvent. Plants, plant tissues and seeds are irrigated, sprayed or soaked, the plant growth and morphogenesis can be effectively promoted, seed germination is quickened, heavy metal accumulation is reduced, and the oxidation resistance and stress resistance / tolerance are improved. The method has the characteristics of no pollution, environmental protection, low cost, and wide application range, and is applied to the fields such as farmland chemical regulation, provenance agriculture, environment and plant nutrition, plant tissue culture and cut flower preservation time prolonging.

The invention discloses a fast tissue culture reproducing method of actinidia eriantha, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) tissue culture medium preparation; (2) explant selection and sterilization; (3) inducing culture; (4) proliferation culture; (5) rooting culture; (6) tissue culture plantlet acclimatization and transplant, and the like. The fast tissue culture reproducing method has high culture medium pertinence and good applicability, the inductivity of an initial sprout of an explant reaches more than 80 percent, the proliferation rate of a successive sprout reaches 2-7 times, the plantlet height can reach 2-5 centimeters for 20-30 days, the quality of tissue culture plantlets is enhanced, the rooting rate reachesmore than 95 percent, and the transplant survival rate reaches more than 95 percent. The invention can be popularized and applied to fruit tree plantlet enterprises.

The invention discloses a Dendrobium in vitro crossbreeding method. The method can be used to greatly shorten the maturation period of fruits, to enable a hybrid to bloom in vitro in a short period so as to observe flower shapes and colors, and to cultivate a novel variety, thus accelerating Dendrobium breeding, the first such report internationally. Since in vitro Dendrobium blooms annually, it enables crossbreeding of varieties having different inflorescences in nature. In addition, the medium used at each stage of the invention utilizes Hyponex which has a unique composition and costs little, thus allowing for a high blossoming rate and rapid fruit development. Also, only simple plant tissue culture equipment is required for implementing the invention, thus the entire breeding method is simple and low cost, and provides conditions for cultivating of high-quality Dendrobium varieties.

The invention discloses a rapid tissue culture and propagation method of curcuma alsimatifolia, belonging to the technical field of plant tissue culture. The method comprises the following steps of: firstly, preparation of a culture medium; secondly, culture and sterilization of earthnut budlet; thirdly, culture of explant sterile seedling; fourthly, induction culture of adventitious bud; fifthly, propagation culture of adventitious bud; sixthly, root growing culture of plantlet; and seventhly, seedling adaptation, acclimation and transplanting of tissue culture seedling. The method disclosedby the invention has the advantages that propagation culture period is short (14-20 days), propagation coefficient is high (improved to 5-8 times from 2-4 times), the consistency of the adventitious bud is good, tissue culture breeding cycle is short (50-60 days), seedling quality is good, and transplanting survival rate can reach up to more than 95%, and is a method for efficiently and rapidly providing high-quality curcuma alsimatifolia seedlings. The method disclosed by the invention can be popularized and applied to landscaping and tissue culture seedling enterprises.

The invention discloses a full-wavelength light source controllable biological culture device. The device comprises a case body the periphery of which is provided with a heat insulating layer, optical wavelength controllable LED (light emitting diode) lamp bead panels arranged on the inner top part and four lateral sides of the case body, temperature and humidity sensors arranged in the case body, a heater and a humidifier arranged at the bottom of the case body, and a miniature quiet compressor. According to the full-wavelength light source controllable and quartz culture flask, biological photo-reaction researches can be performed in plant tissue culture, cell culture, microbiological culture and animal culture ways, the operation is easy, and the light source can be subjected to wavelength combination and illumination time program setting according to different requirements.

The invention discloses an open-type plant tissue culture method. A volatile plant essential oil bacteriostatic agent is added into a culture medium, a high-pressure disinfection process is saved, and inoculation is performed in an indoor open environment; and the culture material is cultured in an environment with the temperature and illumination conditions for plant tissue culture. According to the invention, the volatile bacteriostatic agent can disinfect the space in a bottle while disinfecting the culture medium, thus the culture grows in a relatively sterile environment, and the probability of the culture getting polluted is reduced; a small amount of bacteriostatic agent is added into the culture medium (the total amount is 2mul-2ml / L of culture medium), and the influence on the osmotic pressure of the culture medium is avoided; the components are non-toxic to human body; and the addition amount is small, and the irritant smell is avoided.

The invention relates to the technical field of microbes, and discloses a separation method for plant endophyte. The separation method comprises the following steps: (1) pretreating plant tissue; (2) culturing plant tissue; and (3) separating endophyte. The disclosed separation method for plant endophyte is capable of effectively removing surface bacteria, fungi, actinomyces and other microbes, and avoiding endophyte separation effect caused by mixing of mixed bacteria. Endophyte in the plant tissue recovers growth after tissue culture, and disinfection processing does not need performing in operation of separating endophyte, so that the damage effect of a disinfectant on plant tissue and endophyte is substantially reduced, the survival probability of endophyte is increase, and the number of endophyte obtained through separation is obviously improved.

The fast propagation method of hemp for industrial use is one hemp tissue culture process including the steps of sterilizing seed, obtaining bacteria-free seedling, culturing lateral bud and rooting culture. The hemp tissue culture process has no limitation of seasons, saving in seedling culture field, low production cost, capacity of maintaining all the excellent characteristics of the mother plant and stability of the genetic characteristics. The present invention can form great amount of tube seedling in short period for mass planting of hemp.

The invention discloses a plant tissue culture environmental information monitoring and simulating system. The plant tissue culture environmental information monitoring and simulating system comprises a tissue culture box, a monitoring unit and a simulating unit, wherein the monitoring unit is formed by a collection module, a wireless transmission module and a display processing module. The collection module collects tissue culture environmental information of plants in the tissue culture box, and sends the environmental information to the transmission module. The wireless transmission module uploads the received environmental information to the display processing module. The display processing module stores, processes, displays and indexes the environmental information. The simulating unit carries out manual simulation on the tissue culture environmental information of the plants in the tissue culture box. The plant tissue culture environmental information monitoring and simulating system is convenient to operate, complete in functions, strong in pertinence, capable of achieving intelligent control for the information such as illumination, temperature, carbon dioxide (CO2) and the like in a greenhouse environment, and is suitable for greenhouse plant tissue culture and for various places such as plant tissue culture boxes, illumination culture boxes, phytotrons, facility agriculture, and has expansibility.

The invention discloses a method for rapidly propagating taxus chinensis seedlings, and the method comprises the steps of preparing nutrient soil, nutrient solution, pretreatment solution and rooting solution by using a plant tissue culture principle; filling the nutrient soil into a nutrient bag; drenching the nutrient soil in the nutrient bag by using the nutrient solution; taking a taxus chinensis semi-lignified twig as a propagation material; shearing the taxus chinensis semi-lignified twig into bud-containing subsections which are 0.5-12cm long; inserting the bud-containing subsections into the nutrient bag after being treated with the pretreatment solution; one week later, spraying the rooting solution which is diluted by 1000-1200 times for promoting the rooting; and spraying once every week until the rooting is finished. Through culture and management for 3-4 weeks, the bud-containing subsection roots become a new plant, the survival rate is above 90% and the root system is well developed. The method provided by the invention has the following advantages of being simple for operation, easy for grasping, feasible in four seasons and beneficial for popularization, well solving the technical problem of rapidly propagation of taxus chinensis seedlings, realizing industrial seedling culture, and being conducive to protecting the precious wild taxus chinensis resources of China.

The invention provides a method for propagating stonegarlic quickly and efficiently. The method is used for large-scale production and propagation, and then market requirements are met. The stonegarlic tissue culture comprises steps of A, sterilization of explants, B, inducement of adventitious buds, C, proliferation of adventitious buds, D, rooting culture and E, seedling exercise and transplanting. The stonegarlic is propagated through the method of plant tissue culture, then the propagation cannot be affected by external conditions and can be performed at all seasons, lands occupied by seedlings are saved, the production cost is saved, all excellent characters of a matrix can be preserved, and hereditary characters are stable. By the aid of the method, a lot of excellent test-tube plantlets can be formed in a short time, the scale and industrial production is performed, and industries such as ornamental horticulture and pharmacy can obtain a lot of raw materials.

The invention discloses a collective medium of tissue culture of flower, and the collective medium is adopted in the tissue culture of flower for proliferation and subculture of test-tube plantlet. The collective medium avoids the process that the medium is separately made of various breeds in tissue culture of flower, is simple and easy to operate, and can effectively reduce the process cost, thus having extensive application value and prospect.

The invention discloses a culture method of high diplont rate sporule regeneration plant of broccoli, which belongs to the technical field of plant tissue culture and comprises the following steps: (1), preparing a culture medium; (2), culturing the high diplont rate sporule regeneration plant of broccoli: 1), selecting a donor plant and a bud, 2), sterilizing the bud, 3), pre-treating and culturing the bud, 4), separating, mixing and subpackaging of bud sporule, 5), culturing sporule embryoid, 6), differentiating and culturing a regeneration plant, 7), rooting and transplanting the regeneration plant and 8), detecting ploidy of the regeneration plant. The invention obviously increases the germ extraction rate and the rate of emergence of the sporule culture of the broccoli and the diplont rate of the regeneration plant respectively to 120 embryos / bud, 70 percent and more than 70 percent from common 80 embryos / bud, 50 percent and about 50 percent, thereby improving the breeding efficiency of the broccoli. The method can be popularized and applied to a vegetable breeding department or company.

The invention mainly relates to the field of plant tissue culture and particularly relates to a butterfly orchid anti-browning tissue culture method and an anti-browning culture medium. The tissue culture method comprises the steps of explant disinfection, primary culture, proliferation and differentiation culture, rooting culture, seedling hardening, transplanting and the like, wherein in the primary culture and the proliferation and differentiation culture, extracted solutions of calendulas, lemons, tomatoes, rosemary and the like are added into a corresponding culture medium so as to effectively prevent and treat a common browning problem in butterfly orchid tissue culture; and meanwhile, different types of cutting manners are used for helping to prevent and treat the browning problem at a differentiation phase, so as to form a compound anti-browning method. The method disclosed by the invention has the advantages of high stability, great success rate, greenness and environmental friendliness, and can annularly and repeatedly produce.

Provided is a plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide. The bacteriostatic (antiseptic) agent comprises isothiazolinone, nisin and aqueous extracts of the Chinese herbal medicines of terminalia, groundsel and aromatic madder. When the bacteriostatic (antiseptic) agent is applied in plant tissue culture, 0.35 to 0.5% of the bacteriostatic (antiseptic) agent is added into a medium, which enables fungal and bacterial contamination to a culture to be effectively reduced or prevented and poses little adverse influence on plant growth, thereby enhancing the success rate of plant tissue culture; the bacteriostatic (antiseptic) agent can also be applied in open type plant culture, e.g., in the rooting phase of sound seedlings during a late plant culture stage, the bacteriostatic (antiseptic) agent is directly added into a medium, and the medium can be inoculated in indoor environment with bacteria or a few bacteria without undergoing the step of autoclaving or operating on a superclean bench, thereby simplifying seedling culture in plant tissue culture, enhancing working efficiency, saving a great amount of energy and reducing production cost for plant tissue culture seedlings; therefore, the bacteriostatic (antiseptic) agent has a critical actual application value.

The invention discloses a composition for preventing browning of a plant tissue culture. The composition contains a direct antioxidant against phenolic substances and a plant antioxidase system reinforcing agent, and can play the systematic synergic action of two anti-browning mechanisms during the plant tissue culture process, on the one hand, the anti-oxidation action of the anti-oxidant can be realized against the phenolic substances infiltrating into a culture medium so as to reflect the direct reduction action, on the other hand, the plant antioxidase system reinforcing agent can reinforce the activity of antioxidase in strawberry tissues in plant tissues, and the production and the expansion of basic factors of the browning, namely quinone type substances can be reduced; and the combination of the two can effectively prevent the browning phenomenon in primary culture of strawberries and reduce the browning rate from the conventional level of above 30% to below 20%. The composition disclosed by the invention is widely suitable for various plants which are prone to browning in different tissue culture stages. The invention further discloses a using method of the composition.

The invention relates to an ultrasound-enhanced bioreactor, comprising a liquid state fermentation tank, an ultrasonic transducer, an ultrasonic generator and an ultrasonic fermentation control system, wherein the ultrasonic transducer is fixed on the side wall of the liquid state fermentation tank by bonding, the radiating surface of the ultrasonic transducer is fixed on the planar structure of the tank body of the liquid state fermentation tank by bonding. The ultrasonic fermentation control system comprises a biological fermentation process control unit and an ultrasonic control unit, wherein the biological fermentation process control unit is used for controlling the pH value, dissolved oxygen, temperature, rotating speed, air flow, tank pressure and supplementary material, and carrying out real-time monitoring on the whole fermentation process and conducting data recording and analysis; and the ultrasonic control unit is used for controlling the start of the ultrasonic transducer, the selection of ultrasonic frequencies, the selection of ultrasonic power and ultrasonic generation time in order to achieve optimum ultrasound-enhanced microbes and effects of growth metabolism of plant tissue cultures.

The invention discloses a tissue-culture quick breeding method for iris tectorum, belonging to the technical field of plant tissue culture. The method comprises the following technical steps: (1) preparing a culture medium; (2) culturing iris tectorum detoxified tissue-culture seedlings; and (3) hardening and transplanting the tissue-culture seedlings. With the method, through adjustment on the type and the content of culture medium hormones, addition of antibiotics and other technical measures, the pollution rate of a iris tectorum explant is obviously lowered, and an inoculation survival rate is above 90%; the induction differentiation rate of the explant can be obviously improved to 99% from 70-80% in the prior art; and the tissue-culture strong seedling rate of the iris tectorum is improved. The tissue-culture quick breeding method can be popularized and applied to landscaping and tissue culture enterprises.

The invention provides a method for cultivating plant tissue under non-aseptic condition, wherein the whole procedure of plant tissue cultivation is conducted under the natural ambient condition of non-asepsis, the method consists of adding disinfection and bactericidal agent into the culture media, preparing bacterium suppression culture media, inoculating under the natural ambient condition of non-asepsis, and placing the culture material in natural ambient condition meeting plant tissue culture.

The invention discloses a crossbreeding method in a dendrobium test tube. According to the invention, the crossbreeding method in the dendrobium test tube is adopted, the ripening period of fruits can be shortened greatly, and crossbreeding generations in the test tube can bloom within a short period of time, and flower patterns and colors can be observed, and novel varieties are bred, thereby accelerating the dendrobium breeding speed, and the crossbreeding method is reported for the first time internationally; because dendrobium in the test tube can bloom annually, and can crossbreed with varieties rare in flower seasons in the natural world. Additionally, according to the invention, culture medium in each stage is utilized, 'hyponex' is utilized as the main component, and the cultural media are unique in ingredient, not only are low in cost, but also are high in abloom rate, and the fruits are developed rapidly; moreover, only a simple plant tissue culture device is required to implement the crossbreeding method provided by the invention, and therefore the whole breeding method is simple and convenient, is low in cost, and creates conditions for culturing the high-quality dendrobium variety.

The invention relates to a bacteriostat composition applicable to plant tissue culture. The bacteriostat composition comprises the following components in percentage by volume: 2.5 percent of epsilon-polylysine, 22 to 29.5 percent of isothiazolinone, 10 to 15 percent of traditional Chinese medicine water extracts, 2.5 percent of potassium sorbate, sodium benzoate and the like, 3.5 percent of stabilizing agent and 47.0 to 59.5 percent of deionized water. When the composition is used for disinfecting explants of plants, the explants are subjected to routine disinfection after being soaked in the bacteriostat, so that the treatment time on corrosive sublimate or sodium hypochlorite can be reduced and the aims of sterilizing and keeping relatively high survival rate of the explants are fulfilled; the bacteriostat is added into a first generation culture medium or a subculture medium, so pollution of fungus and bacteria can be effectively reduced and prevented and the bacteriostat does not have adverse effect on the plant growth; and when applied to plant open culture, such as a strong seeding and rooting stage and the like, the bacteriostat composition can save the culture medium high-pressure sterilizing process, does not need a clean bench, can save energy and reduce the cost of tissue culture seedlings, and improves benefits.

The invention relates to a method for producing the lycoris by plant tissue culture, which comprises the steps of: (1) taking an MS (mass spectrometry) culture medium as a basic culture medium, and taking a 2, 4-D, IAA (indo acetic acid), NAA (naphthyl acetic acid), 6-BA (butyl acrylate) and IBA (iso butyl alcohol) as a regulated and controlled growth hormone preparation culture medium; (2) taking the bulb of the lycoris as an explant, and sterilizing; (3) vaccinating the explant of the bulb on a bulb inducing culture medium to culture in an inducing way so as to grow bulbils; (4) transferring the bulbils into a rooting culture medium to culture in a rooting way, so that the bulbils can independently absorb the nutrient elements from the soil and the matrix; (5) transferring the bulbils into the culture medium to culture; (6) opening an opening of a vaccination bottle to refine the seedling; withdrawing the bulbils, and transferring the bulbils into a nutrient cup which takes the vermiculite, the pearlite and the nutrient soil as the mixed matrix; and transferring the bulbils into a large tent, so that the survival rate reaches 94%-96%. The method is less in material taking, and economical in culturing; the culture condition can be manually controlled and is not influenced by the natural condition; the method is short in growing period, and high in propagation rate; and the quality of lycoris is guaranteed.

The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.

The invention belongs to the technical field of plant tissue culture and particularly relates to a high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture. Primary inducing culture medium used in the method is improved WPM+Zt 1.0-1.5mg / L, successive multiplication culture medium is improved WPM+Zt 0.5-0.8mg / L, and rooting culture medium is 1 / 2 improved WPM+IAA 0.1-0.2mg / L+AC 0.5g / L. The method comprises the steps of: dipping the stem bases of successively multiplied aseptic blueberry seedlings in IBA sterile solution with concentration being 100-500mg / L for 5-15 seconds, inoculating the stem bases in the blueberry rooting culture medium, moving a rooting tissue culture seedling culture bottle from a culture room to a plastic greenhouse for training seedlings for 4-7 days after rooting, uncovering the plastic greenhouse and training the seedlings for 3-5 days before transplanting, transplanting the seedlings in culture medium in a cell tray, laying a plastic film to preserve moisture for 5-7 days, ventilating twice a day for 5-15 minutes per time during moisture preservation and fully removing the plastic film after 7 days. The high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture has the advantages that not only is the efficiency high, but also the cost is lower and the mass production is facilitated.